CN109287499A - A kind of cat litter and preparation method thereof with pet Urine proteins warning function - Google Patents
A kind of cat litter and preparation method thereof with pet Urine proteins warning function Download PDFInfo
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- CN109287499A CN109287499A CN201811247499.0A CN201811247499A CN109287499A CN 109287499 A CN109287499 A CN 109287499A CN 201811247499 A CN201811247499 A CN 201811247499A CN 109287499 A CN109287499 A CN 109287499A
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- 210000002700 urine Anatomy 0.000 title claims abstract description 133
- 241000282326 Felis catus Species 0.000 title claims abstract description 124
- 108090000623 proteins and genes Proteins 0.000 title claims abstract description 114
- 102000004169 proteins and genes Human genes 0.000 title claims abstract description 114
- 238000002360 preparation method Methods 0.000 title claims abstract description 23
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims abstract description 152
- 239000000741 silica gel Substances 0.000 claims abstract description 35
- 229910002027 silica gel Inorganic materials 0.000 claims abstract description 35
- 239000003242 anti bacterial agent Substances 0.000 claims abstract description 30
- OHJMTUPIZMNBFR-UHFFFAOYSA-N biuret Chemical compound NC(=O)NC(N)=O OHJMTUPIZMNBFR-UHFFFAOYSA-N 0.000 claims abstract description 30
- 229910052799 carbon Inorganic materials 0.000 claims abstract description 23
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims abstract description 21
- 239000002994 raw material Substances 0.000 claims abstract description 9
- 239000002245 particle Substances 0.000 claims description 90
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 84
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 60
- 239000012153 distilled water Substances 0.000 claims description 60
- XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 claims description 48
- 238000000034 method Methods 0.000 claims description 43
- 229960001866 silicon dioxide Drugs 0.000 claims description 34
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 32
- UDSAIICHUKSCKT-UHFFFAOYSA-N bromophenol blue Chemical group C1=C(Br)C(O)=C(Br)C=C1C1(C=2C=C(Br)C(O)=C(Br)C=2)C2=CC=CC=C2S(=O)(=O)O1 UDSAIICHUKSCKT-UHFFFAOYSA-N 0.000 claims description 31
- 238000001291 vacuum drying Methods 0.000 claims description 28
- 239000000377 silicon dioxide Substances 0.000 claims description 25
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims description 24
- 230000000845 anti-microbial effect Effects 0.000 claims description 21
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims description 16
- 238000007711 solidification Methods 0.000 claims description 16
- 230000008023 solidification Effects 0.000 claims description 16
- 238000001035 drying Methods 0.000 claims description 14
- 239000003153 chemical reaction reagent Substances 0.000 claims description 12
- 239000003795 chemical substances by application Substances 0.000 claims description 12
- 238000003756 stirring Methods 0.000 claims description 11
- ARUVKPQLZAKDPS-UHFFFAOYSA-L copper(II) sulfate Chemical compound [Cu+2].[O-][S+2]([O-])([O-])[O-] ARUVKPQLZAKDPS-UHFFFAOYSA-L 0.000 claims description 10
- 238000010438 heat treatment Methods 0.000 claims description 10
- VZOPRCCTKLAGPN-ZFJVMAEJSA-L potassium;sodium;(2r,3r)-2,3-dihydroxybutanedioate;tetrahydrate Chemical compound O.O.O.O.[Na+].[K+].[O-]C(=O)[C@H](O)[C@@H](O)C([O-])=O VZOPRCCTKLAGPN-ZFJVMAEJSA-L 0.000 claims description 10
- 229940074446 sodium potassium tartrate tetrahydrate Drugs 0.000 claims description 10
- 230000008569 process Effects 0.000 claims description 9
- QTBSBXVTEAMEQO-UHFFFAOYSA-M Acetate Chemical compound CC([O-])=O QTBSBXVTEAMEQO-UHFFFAOYSA-M 0.000 claims description 8
- 238000002604 ultrasonography Methods 0.000 claims description 8
- 238000004090 dissolution Methods 0.000 claims description 6
- 241001062009 Indigofera Species 0.000 claims description 5
- JDZJVWAHZYIHFA-UHFFFAOYSA-N [Br].C1(=CC=CC=C1)O Chemical compound [Br].C1(=CC=CC=C1)O JDZJVWAHZYIHFA-UHFFFAOYSA-N 0.000 claims description 3
- KLZMUJRJFXYDCW-UHFFFAOYSA-N 2-[6-(diaminomethylideneamino)hexyl]guanidine;hydrochloride Chemical compound Cl.NC(N)=NCCCCCCN=C(N)N KLZMUJRJFXYDCW-UHFFFAOYSA-N 0.000 claims description 2
- WKBOTKDWSSQWDR-UHFFFAOYSA-N Bromine atom Chemical compound [Br] WKBOTKDWSSQWDR-UHFFFAOYSA-N 0.000 claims description 2
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 claims description 2
- GDTBXPJZTBHREO-UHFFFAOYSA-N bromine Substances BrBr GDTBXPJZTBHREO-UHFFFAOYSA-N 0.000 claims description 2
- 229910052794 bromium Inorganic materials 0.000 claims description 2
- 238000002386 leaching Methods 0.000 claims description 2
- 239000011734 sodium Substances 0.000 claims description 2
- XUIMIQQOPSSXEZ-UHFFFAOYSA-N Silicon Chemical compound [Si] XUIMIQQOPSSXEZ-UHFFFAOYSA-N 0.000 claims 1
- 239000003292 glue Substances 0.000 claims 1
- 239000011148 porous material Substances 0.000 claims 1
- 229910052710 silicon Inorganic materials 0.000 claims 1
- 239000010703 silicon Substances 0.000 claims 1
- 238000006243 chemical reaction Methods 0.000 abstract description 14
- 230000000694 effects Effects 0.000 abstract description 6
- 238000004043 dyeing Methods 0.000 abstract description 5
- 230000036541 health Effects 0.000 abstract description 5
- 239000000463 material Substances 0.000 abstract description 3
- 230000001408 fungistatic effect Effects 0.000 abstract description 2
- 238000011835 investigation Methods 0.000 abstract description 2
- 239000000758 substrate Substances 0.000 abstract description 2
- 230000010148 water-pollination Effects 0.000 abstract description 2
- 230000006870 function Effects 0.000 description 29
- 230000008859 change Effects 0.000 description 17
- 125000004836 hexamethylene group Chemical group [H]C([H])([*:2])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])[*:1] 0.000 description 13
- 238000005303 weighing Methods 0.000 description 9
- 230000008676 import Effects 0.000 description 8
- 238000007689 inspection Methods 0.000 description 8
- 229920002413 Polyhexanide Polymers 0.000 description 6
- BNIILDVGGAEEIG-UHFFFAOYSA-L disodium hydrogen phosphate Chemical compound [Na+].[Na+].OP([O-])([O-])=O BNIILDVGGAEEIG-UHFFFAOYSA-L 0.000 description 6
- 229910000397 disodium phosphate Inorganic materials 0.000 description 6
- 235000019800 disodium phosphate Nutrition 0.000 description 6
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 3
- 239000004202 carbamide Substances 0.000 description 3
- 230000006378 damage Effects 0.000 description 3
- 230000001877 deodorizing effect Effects 0.000 description 3
- 208000027418 Wounds and injury Diseases 0.000 description 2
- 230000009102 absorption Effects 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 2
- 150000001875 compounds Chemical class 0.000 description 2
- 238000004821 distillation Methods 0.000 description 2
- 208000014674 injury Diseases 0.000 description 2
- 210000003734 kidney Anatomy 0.000 description 2
- 230000000051 modifying effect Effects 0.000 description 2
- 239000000843 powder Substances 0.000 description 2
- 239000002023 wood Substances 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 206010059410 Faecaluria Diseases 0.000 description 1
- 206010063897 Renal ischaemia Diseases 0.000 description 1
- 230000000274 adsorptive effect Effects 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 239000000022 bacteriostatic agent Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000000440 bentonite Substances 0.000 description 1
- 229910000278 bentonite Inorganic materials 0.000 description 1
- SVPXDRXYRYOSEX-UHFFFAOYSA-N bentoquatam Chemical class O.O=[Si]=O.O=[Al]O[Al]=O SVPXDRXYRYOSEX-UHFFFAOYSA-N 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 239000004927 clay Chemical class 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 238000009535 clinical urine test Methods 0.000 description 1
- 230000018044 dehydration Effects 0.000 description 1
- 238000006297 dehydration reaction Methods 0.000 description 1
- 238000004332 deodorization Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 239000000428 dust Substances 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 230000000855 fungicidal effect Effects 0.000 description 1
- 239000000417 fungicide Substances 0.000 description 1
- 229910052736 halogen Inorganic materials 0.000 description 1
- 150000002367 halogens Chemical class 0.000 description 1
- 230000003862 health status Effects 0.000 description 1
- 229910001385 heavy metal Inorganic materials 0.000 description 1
- 230000001771 impaired effect Effects 0.000 description 1
- 230000003907 kidney function Effects 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 210000002381 plasma Anatomy 0.000 description 1
- 230000003389 potentiating effect Effects 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 230000009103 reabsorption Effects 0.000 description 1
- 230000008085 renal dysfunction Effects 0.000 description 1
- 238000011012 sanitization Methods 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 208000024891 symptom Diseases 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K1/00—Housing animals; Equipment therefor
- A01K1/015—Floor coverings, e.g. bedding-down sheets ; Stable floors
- A01K1/0152—Litter
- A01K1/0155—Litter comprising organic material
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K1/00—Housing animals; Equipment therefor
- A01K1/015—Floor coverings, e.g. bedding-down sheets ; Stable floors
- A01K1/0152—Litter
- A01K1/0154—Litter comprising inorganic material
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Chemical & Material Sciences (AREA)
- Inorganic Chemistry (AREA)
- Housing For Livestock And Birds (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The present invention discloses a kind of cat litter and preparation method thereof with pet Urine proteins warning function.Cat litter of the present invention is prepared by following raw material: modified Kiselgel A, diatomite, active carbon, Urine proteins indicator and antibacterial agent.Biuret reaction is applied in the preparation process of cat litter by the present invention, the dosage of scientific matching silica gel substrate material and Urine proteins indicator, it is set to can produce chromogenic reaction for the urine of Urine proteins exception, and to the urine of normal value without chromogenic reaction, it is possible thereby to tentatively reflect pet health situation, it can go to a doctor in time investigation for the pet of urine exception, play forewarning function.The present invention is also modified silica gel, effectively increases its hydrophily, enhances its dip dyeing effect with Urine proteins indicator, distinguishes chromogenic reaction obviously easily.Antibacterial agent is added simultaneously, keeps cat litter fungistatic effect of the present invention significant, and duration is good, does not need often to replace.Preparation process of the present invention is simple, low in cost, is suitble to promote the use of.
Description
Technical field
The invention belongs to cat litter preparation technical fields, and in particular to a kind of silica gel cat litter with cat Urine proteins warning function
And preparation method thereof.
Background technique
Cat litter is a kind of pet supplies that consumption is very big, with social development and the raising of living standards of the people, family
The quantity for raising the pets such as cat and dog is increasing, and the sanitized of fecaluria becomes the important topic of environmental protection, cat litter then at
For the common necessary article of most raising pet families.
Common cat litter has bentonite, clay class, paper scrap class, wood powder class and silica gel cat litter etc. currently on the market, but by
In POLARGEL NF class cat litter deodorizing capability is not ideal enough, to be also easy to produce mildew, dust more after dampness;Paper scrap class cat litter coagulability
Weak, the disadvantages of mutability is at a paste after the moisture absorption, user's cleaning is got up little convenience.Wood powder cat litter is more environmentally friendly, but production at present
Producer is less.
Silica gel cat litter uses silica gel as pet padding.Silica gel cat litter is that a kind of latest model, optimal refuse of pets are clear
Clean dose, main component is silica, and content is up to 98% or more, and white in appearance graininess, family is high using degree of safety,
It is to compare the cat litter product welcome by the majority of consumers on current international market.Existing silica gel cat litter, major function are to inhale
Attached deodorization, has a single function limited, and cost is higher.Necessity is wanted to develop very much a kind of low in cost, preparation process is simple, in height
While imitating absorbing and deodorizing, it can reflect the cat litter product of pet health exception.
Summary of the invention
To solve the problems, such as that existing cat litter product cost is high, performance is bad and has a single function, the present invention provides one
Kind has the cat litter and preparation method thereof of pet Urine proteins warning function, with Kiselgel A base material the most, and carries out to it
Hydrophilic modifying enhances the dip dyeing effect of protein indicator；Diatomite active carbon is added, the absorbing and deodorizing energy of silica gel cat litter is enhanced
Power；Novel double lonely class antibacterial agents are added, realize high-efficiency antimicrobial bacteria resistance function.Purpose to realize the present invention, used technical side
Case is:
A kind of cat litter with pet Urine proteins warning function, is made of raw material from the following weight: modified Kiselgel A 60-
100 parts, 5-10 parts of diatomite, 5-10 parts of active carbon, 100-150 parts of Urine proteins indicator and 0.1-0.5 parts of antibacterial agent；The urine
Protein indicator is bromophenol blue reagent or biuret reagent.
The bromophenol blue reagent is prepared by following methods: weighing the 0.1mol/ that bromophenol blue 0.67g is dissolved in 10ml
In L NaOH solution, add distilled water to 1000ml.
The biuret reagent is prepared by following methods: being weighed 1.5g copper sulphate and 6.0g sodium potassium tartrate tetrahydrate, is used
The dissolution of 500mL distilled water, 300mL10% sodium hydroxide solution is added under stiring, is diluted with water to 1L；
The modified Kiselgel A is prepared by following methods:
(1) silica gel particle is dipped in the ethanol solution that mass fraction is 60%, is cleaned by ultrasonic 30min, wash with distilled water postposition
35-40 DEG C of dry 30min in a vacuum drying oven, it is spare；
(2) step (1) silica obtained particle is dipped in 10-15min in ethyl acetate, it is 15% that mass fraction is dipped in after rear taking-up
Polycarbodiimide solution in 2min, after in 37 DEG C of solidification 30min obtain modified silica-gel particle.
The antibacterial agent is hexamethylene.
A kind of preparation method of the cat litter with pet Urine proteins warning function, comprising the following steps:
(1) it pre-processes: in 0.1mol/L citric acid solution 1000m1,0.2mol/L Na is added2HPO4Solution 20ml adjusts pH
It is 2.1, buffer is prepared；Kiselgel A is soaked in hydrochloric acid solution after 30min and is taken out, after distilled water cleans 3-5 times
It is soaked in gained buffer and takes out after 30min, dry after 3-5 times wash with distilled water；
(2) it prepares modified silica-gel: step (1) silica obtained particle being dipped in the ethanol solution that mass fraction is 60%, ultrasound is clear
30min is washed, wash with distilled water postposition 35-40 DEG C of dry 30min in a vacuum drying oven, then silica obtained particle is dipped in second
10-15min in acetate solution, it is rear take out after be dipped in 2min in the polycarbodiimide solution that mass fraction is 15%, after in 37
DEG C solidification 30min obtain modified silica-gel particle；
(3) prepare Urine proteins indicator: biuret reagent is prepared, and is weighed 1.5g copper sulphate and 6.0g sodium potassium tartrate tetrahydrate, is used 500mL
Distilled water dissolution, 300mL10% sodium hydroxide solution is added under stiring, is diluted with water to 1L；Bromophenol blue trial system, weighs bromine
Phenol indigo plant 0.67g is dissolved in the 0.1mol/L NaOH solution of 10ml, adds distilled water to 1000ml；
(4) modified silica-gel and biuret Urine proteins indicator are weighed according to the ratio, and modified silica-gel is dipped in the instruction of biuret Urine proteins
It is stirred in agent solution, and at 50 DEG C after heating stirring 2-5h, filters to isolate silica gel particle, juxtaposition is true in a vacuum drying oven
Sky is dry, obtains first product A1；Modified silica-gel and bromophenol blue Urine proteins indicator are weighed according to the ratio, and modified silica-gel is dipped in bromine
It is stirred in phenol indigo plant Urine proteins indicator solution, and filters to isolate silica gel particle after heating stirring 2-5h at 50 DEG C, be placed in
It is dried in vacuo in vacuum oven, obtains first product A2；
(5) it takes first product A1 obtained by the diatomite, active carbon and step (4) of the weight to be mixed, is then placed in blender
Middle homogenizing obtains hybrid particles B1；
(6) antibacterial agent of parts by weight is dissolved in the distilled water of 50-100 times of weight, obtains antimicrobial；By step (5) institute
It obtains hybrid particles B1 to be placed in rotating disk, and to antimicrobial is uniformly sprayed thereon, particle is placed in vacuum after the completion of sprinkling
Drying box is dry, until water content 4-8%, is then packed to get finished product cat litter C1；
(7) it takes first product A2 obtained by the diatomite, active carbon and step (4) of the weight to be mixed, is then placed in blender
Middle homogenizing obtains hybrid particles B2；
(8) antibacterial agent of parts by weight is dissolved in the distilled water of 50-100 times of weight, obtains antimicrobial；It will be mixed obtained by step
It closes particle B2 to be placed in rotating disk, and to antimicrobial is uniformly sprayed thereon, particle is placed in vacuum drying after the completion of sprinkling
Case is dry, until water content 4-8%, is then packed to get finished product cat litter C2.
The mass fraction of step (1) ethyl acetate solution is 60-80%.
The temperature of vacuum oven is 100 DEG C in step (3), and drying time is 12 hours.
Using the cat litter with pet Urine proteins warning function in application, colorimetric card can be made for convenience of use,
With rapid comparison color change.Colorimetric card can be prepared by the following method:
(1) it is taken pictures to finished product cat litter C1 and imports computer；The exceeded cat urine of Urine proteins, normal cats urine is taken to infiltrate finished product respectively
Color change result take pictures importing computer by cat litter C1, carries out color difference inspection in computer internal reference piece, passes through analysis
Obtain corresponding rgb value；Colorimetric card is made according to corresponding rgb value；
(2) it is taken pictures to finished product cat litter C2 and imports computer；The exceeded cat urine of Urine proteins, normal cats urine is taken to infiltrate finished product respectively
Color change result take pictures importing computer by cat litter C2, carries out color difference inspection in computer internal reference piece, passes through analysis
Obtain corresponding rgb value；Colorimetric card is made according to corresponding rgb value.
The method that wherein color difference is examined is:
(1) according to each color rgb value in Raul's colorimetric card, Raul's colorimetric card of electronic edition is generated in a computer；
(2) cat litter taken pictures and Raul's colorimetric card in kind will be needed to be placed in together, imported after being taken pictures under same environment
Computer；Whether the rgb value for then verifying the rgb value of Raul's colorimetric card in kind and standard Raul's colorimetric card of electronic edition is consistent,
When consistent, illustrate the conclusion of the rgb value no color differnece of the photo of clapped cat litter.
When practical application, pet is drained into the silica gel color change after use and colorimetric card compares, with quick discrimination pet
Whether urine protein content is normal in urine, and using biuret reagent as urea protein indicator, when Urine proteins are exceeded, silica gel can be by
Blue becomes purple.Using bromophenol blue reagent as urea protein indicator, when Urine proteins are exceeded, silica gel can be become blue from yellow
Color.
The histopathology performance that Urine proteins are formed is mainly glomerulus expansion, the damage of capillary inner cell and then blood plasma egg
It is white to be leaked out from glomerulus, while renal ischemia leads to reabsorption miopragia, when Urine proteins leaching is more than weight
Just Urine proteins is caused to be formed when uptake.Thus, Urine proteins can generally reflect Impaired kidney function evil degree, be provided simultaneously with preliminary
Prediction injury of kidney and the effect for making a definite diagnosis injury of kidney symptom.Therefore, pet can be reacted by detecting pet urine urine protein content
Health condition.Urine proteins can generate metachromatism in conjunction with a variety of chemical reagent, therefore can pass through this metachromatism
Reaction tentatively judges whether pet suffers from disease.
The present invention selects biuret and bromophenol blue as Urine proteins indicator.Biuret reaction is that peptide and protein institute are peculiar
, with two or more peptide bonds compound under alkaline condition with Cu2+Reaction, generates the complex compound of reddish violet, molten
Liquid color is by basket purpling.Protein indicator used in the present invention exactly utilizes this chromogenic reaction, is applied to cat litter preparation
In technique, the health status of pet can be predicted by the way that color change whether occurs in cat litter use process, play warning and prevent
Model effect.Simultaneously.For the dip dyeing effect for promoting protein indicator, commercially available resulting Kiselgel A is modified, mentions by the present invention
Its high hydrophilicity, disseminates protein indicator efficiently on silica gel particle.
The present invention selects hexamethylene bis guanidine hydrochloride as cat litter bacteriostatic agent, can effective bactericidal, and compare
Other solvent type fungicide compare, and cooperate with silica gel particle, it is good, right will not to generate strong pungent smell, compatibility
The toxicity of pet is low, can be used for a long time, and is free of heavy metal and organic halogen, not will cause adverse effect to environment.
To sum up, the beneficial effects of the present invention are: biuret reaction is applied in the preparation process of cat litter by the present invention, science
Silica gel substrate material and biuret reagent are matched, it is made to can produce chromogenic reaction for the urine of Urine proteins exception, and to normal
The urine of value, it is possible thereby to tentatively reflect pet health situation, can go to a doctor for the pet of urine exception in time without chromogenic reaction
Investigation, plays forewarning function.The diatomite and active carbon of addition are strong adsorptive porous substance, can potent absorption water and peculiar smell,
Purify air.The present invention is also modified silica gel, effectively increases its hydrophily, enhances its dip dyeing with biuret reagent, makes
Chromogenic reaction is obviously easily distinguished.Antibacterial agent is added simultaneously, keeps cat litter fungistatic effect of the present invention significant, and duration is good, no
It needs to be replaced frequently.Preparation process of the present invention is simple, low in cost, is suitble to promote the use of.
Detailed description of the invention
Fig. 1 be the present invention using biuret reagent as protein indicator when prepared standard color comparison card.
Specific embodiment
Technical scheme is described further combined with specific embodiments below, but not limited to this.
Embodiment 1
A kind of cat litter with pet Urine proteins warning function, is made of raw material from the following weight: modified 60 parts of Kiselgel A,
5 parts of diatomite, 5 parts of active carbon, 100 parts of Urine proteins indicator and 0.1 part of antibacterial agent；The Urine proteins indicator is biuret examination
Agent.
The biuret reagent is prepared by following methods: being weighed 1.5g copper sulphate and 6.0g sodium potassium tartrate tetrahydrate, is used
The dissolution of 500mL distilled water, 300mL10% sodium hydroxide solution is added under stiring, is diluted with water to 1L；
The modified Kiselgel A is prepared by following methods:
(1) silica gel particle is dipped in the ethanol solution that mass fraction is 60%, is cleaned by ultrasonic 30min, wash with distilled water postposition
35-40 DEG C of dry 30min in a vacuum drying oven, it is spare；
(2) step (1) silica obtained particle is dipped in 10-15min in ethyl acetate, it is 15% that mass fraction is dipped in after rear taking-up
Polycarbodiimide solution in 2min, after in 37 DEG C of solidification 30min obtain modified silica-gel particle.
The antibacterial agent is hexamethylene.Hexamethylene is also known as PHMB, purchased from upper
Hai Gaoju Biotechnology Co., Ltd, active principle content are 20%.
A kind of preparation method of the cat litter with pet Urine proteins warning function, comprising the following steps:
(1) it pre-processes: in 0.1mol/L citric acid solution 1000m1,0.2mol/L Na2HPO4 solution 20ml is added, adjusts pH
It is 2.1, buffer is prepared；Kiselgel A is soaked in hydrochloric acid solution after 30min and is taken out, after distilled water cleans 3-5 times
It is soaked in gained buffer and takes out after 30min, dry after 3-5 times wash with distilled water；
(2) it prepares modified silica-gel: step (1) silica obtained particle being dipped in the ethanol solution that mass fraction is 60%, ultrasound is clear
30min is washed, wash with distilled water postposition 35-40 DEG C of dry 30min in a vacuum drying oven, then silica obtained particle is dipped in second
10-15min in acetate solution, it is rear take out after be dipped in 2min in the polycarbodiimide solution that mass fraction is 15%, after in 37
DEG C solidification 30min obtain modified silica-gel particle；
(3) it prepares Urine proteins indicator: weighing 1.5g copper sulphate and 6.0g sodium potassium tartrate tetrahydrate, dissolved, stirred with 500mL distilled water
Lower addition 300mL10% sodium hydroxide solution is mixed, 1L is diluted with water to；
(4) modified silica-gel and biuret Urine proteins indicator are weighed according to the ratio, and modified silica-gel is dipped in the instruction of biuret Urine proteins
It is stirred in agent solution, and at 50 DEG C after heating stirring 2-5h, filters to isolate silica gel particle, juxtaposition is true in a vacuum drying oven
Sky is dry, obtains first product A1；
(5) it takes first product A1 obtained by the diatomite, active carbon and step (4) of the weight to be mixed, is then placed in blender
Middle homogenizing obtains hybrid particles B1；
(6) antibacterial agent of parts by weight is dissolved in the distilled water of 50-100 times of weight, obtains antimicrobial；By step (5) institute
It obtains hybrid particles B1 to be placed in rotating disk, and to antimicrobial is uniformly sprayed thereon, particle is placed in vacuum after the completion of sprinkling
Drying box is dry, until water content 4-8%, is then packed to get finished product cat litter C1；
The mass fraction of step (1) ethyl acetate solution is 60-80%.
The temperature of vacuum oven is 100 DEG C in step (3), and drying time is 12 hours.
Using the cat litter with pet Urine proteins warning function in application, colorimetric card can be made for convenience of use,
With rapid comparison color change.Colorimetric card can be prepared by the following method:
(1) it is taken pictures to finished product cat litter C1 and imports computer；The exceeded cat urine of Urine proteins, normal cats urine is taken to infiltrate finished product respectively
Color change result take pictures importing computer by cat litter C1, carries out color difference inspection in computer internal reference piece, passes through analysis
Obtain corresponding rgb value；Colorimetric card is made according to corresponding rgb value；
The method that wherein color difference is examined is:
(1) according to each color rgb value in Raul's colorimetric card, Raul's colorimetric card of electronic edition is generated in a computer；
(2) cat litter taken pictures and Raul's colorimetric card in kind will be needed to be placed in together, imported after being taken pictures under same environment
Computer；Whether the rgb value for then verifying the rgb value of Raul's colorimetric card in kind and standard Raul's colorimetric card of electronic edition is consistent
When, when consistent, illustrate the conclusion of the rgb value no color differnece of the photo of clapped cat litter.
Embodiment 2
A kind of cat litter with pet Urine proteins warning function, is made of raw material from the following weight: modified 80 parts of Kiselgel A,
8 parts of diatomite, 8 parts of active carbon, 130 parts of Urine proteins indicator and 0.3 part of antibacterial agent；The Urine proteins indicator is biuret examination
Agent.
The biuret reagent is prepared by following methods: being weighed 1.5g copper sulphate and 6.0g sodium potassium tartrate tetrahydrate, is used
The dissolution of 500mL distilled water, 300mL10% sodium hydroxide solution is added under stiring, is diluted with water to 1L；
The modified Kiselgel A is prepared by following methods:
(1) silica gel particle is dipped in the ethanol solution that mass fraction is 60%, is cleaned by ultrasonic 30min, wash with distilled water postposition
35-40 DEG C of dry 30min in a vacuum drying oven, it is spare；
(2) step (1) silica obtained particle is dipped in 10-15min in ethyl acetate, it is 15% that mass fraction is dipped in after rear taking-up
Polycarbodiimide solution in 2min, after in 37 DEG C of solidification 30min obtain modified silica-gel particle.
The antibacterial agent is hexamethylene.Hexamethylene is also known as PHMB, purchased from upper
Hai Gaoju Biotechnology Co., Ltd, active principle content are 20%.
A kind of preparation method of the cat litter with pet Urine proteins warning function, comprising the following steps:
(1) it pre-processes: in 0.1mol/L citric acid solution 1000m1,0.2mol/L Na2HPO4 solution 20ml is added, adjusts pH
It is 2.1, buffer is prepared；Kiselgel A is soaked in hydrochloric acid solution after 30min and is taken out, after distilled water cleans 3-5 times
It is soaked in gained buffer and takes out after 30min, dry after 3-5 times wash with distilled water；
(2) it prepares modified silica-gel: step (1) silica obtained particle being dipped in the ethanol solution that mass fraction is 60%, ultrasound is clear
30min is washed, wash with distilled water postposition 35-40 DEG C of dry 30min in a vacuum drying oven, then silica obtained particle is dipped in second
10-15min in acetate solution, it is rear take out after be dipped in 2min in the polycarbodiimide solution that mass fraction is 15%, after in 37
DEG C solidification 30min obtain modified silica-gel particle；
(3) it prepares Urine proteins indicator: weighing 1.5g copper sulphate and 6.0g sodium potassium tartrate tetrahydrate, dissolved, stirred with 500mL distilled water
Lower addition 300mL10% sodium hydroxide solution is mixed, 1L is diluted with water to；
(4) modified silica-gel and biuret Urine proteins indicator are weighed according to the ratio, and modified silica-gel is dipped in the instruction of biuret Urine proteins
It is stirred in agent solution, and at 50 DEG C after heating stirring 2-5h, filters to isolate silica gel particle, juxtaposition is true in a vacuum drying oven
Sky is dry, obtains first product A1；
(5) it takes first product A1 obtained by the diatomite, active carbon and step (4) of the weight to be mixed, is then placed in blender
Middle homogenizing obtains hybrid particles B1；
(6) antibacterial agent of parts by weight is dissolved in the distilled water of 50-100 times of weight, obtains antimicrobial；By step (5) institute
It obtains hybrid particles B1 to be placed in rotating disk, and to antimicrobial is uniformly sprayed thereon, particle is placed in vacuum after the completion of sprinkling
Drying box is dry, until water content 4-8%, is then packed to get finished product cat litter C1；
The mass fraction of step (1) ethyl acetate solution is 60-80%.
The temperature of vacuum oven is 100 DEG C in step (3), and drying time is 12 hours.
Using the cat litter with pet Urine proteins warning function in application, colorimetric card can be made for convenience of use,
With rapid comparison color change.Colorimetric card can be prepared by the following method:
(1) it is taken pictures to finished product cat litter C1 and imports computer；The exceeded cat urine of Urine proteins, normal cats urine is taken to infiltrate finished product respectively
Color change result take pictures importing computer by cat litter C1, carries out color difference inspection in computer internal reference piece, passes through analysis
Obtain corresponding rgb value；Colorimetric card is made according to corresponding rgb value；
The method that wherein color difference is examined is:
(1) according to each color rgb value in Raul's colorimetric card, Raul's colorimetric card of electronic edition is generated in a computer；
(2) cat litter taken pictures and Raul's colorimetric card in kind will be needed to be placed in together, imported after being taken pictures under same environment
Computer；Whether the rgb value for then verifying the rgb value of Raul's colorimetric card in kind and standard Raul's colorimetric card of electronic edition is consistent
When, when consistent, illustrate the conclusion of the rgb value no color differnece of the photo of clapped cat litter.
Embodiment 3
A kind of cat litter with pet Urine proteins warning function, is made of raw material from the following weight: modified Kiselgel A 100
Part, 10 parts of diatomite, 10 parts of active carbon, 150 parts of Urine proteins indicator and 0.5 part of antibacterial agent；The Urine proteins indicator is double
Contracting urea reagent.
The biuret reagent is prepared by following methods: being weighed 1.5g copper sulphate and 6.0g sodium potassium tartrate tetrahydrate, is used
The dissolution of 500mL distilled water, 300mL10% sodium hydroxide solution is added under stiring, is diluted with water to 1L；
The modified Kiselgel A is prepared by following methods:
(1) silica gel particle is dipped in the ethanol solution that mass fraction is 60%, is cleaned by ultrasonic 30min, wash with distilled water postposition
35-40 DEG C of dry 30min in a vacuum drying oven, it is spare；
(2) step (1) silica obtained particle is dipped in 10-15min in ethyl acetate, it is 15% that mass fraction is dipped in after rear taking-up
Polycarbodiimide solution in 2min, after in 37 DEG C of solidification 30min obtain modified silica-gel particle.
The antibacterial agent is hexamethylene.Hexamethylene is also known as PHMB, purchased from upper
Hai Gaoju Biotechnology Co., Ltd, active principle content are 20%.
A kind of preparation method of the cat litter with pet Urine proteins warning function, comprising the following steps:
(1) it pre-processes: in 0.1mol/L citric acid solution 1000m1,0.2mol/L Na2HPO4 solution 20ml is added, adjusts pH
It is 2.1, buffer is prepared；Kiselgel A is soaked in hydrochloric acid solution after 30min and is taken out, after distilled water cleans 3-5 times
It is soaked in gained buffer and takes out after 30min, dry after 3-5 times wash with distilled water；
(2) it prepares modified silica-gel: step (1) silica obtained particle being dipped in the ethanol solution that mass fraction is 60%, ultrasound is clear
30min is washed, wash with distilled water postposition 35-40 DEG C of dry 30min in a vacuum drying oven, then silica obtained particle is dipped in second
10-15min in acetate solution, it is rear take out after be dipped in 2min in the polycarbodiimide solution that mass fraction is 15%, after in 37
DEG C solidification 30min obtain modified silica-gel particle；
(3) it prepares Urine proteins indicator: weighing 1.5g copper sulphate and 6.0g sodium potassium tartrate tetrahydrate, dissolved, stirred with 500mL distilled water
Lower addition 300mL10% sodium hydroxide solution is mixed, 1L is diluted with water to；
(4) modified silica-gel and biuret Urine proteins indicator are weighed according to the ratio, and modified silica-gel is dipped in the instruction of biuret Urine proteins
It is stirred in agent solution, and at 50 DEG C after heating stirring 2-5h, filters to isolate silica gel particle, juxtaposition is true in a vacuum drying oven
Sky is dry, obtains first product A1；
(5) it takes first product A1 obtained by the diatomite, active carbon and step (4) of the weight to be mixed, is then placed in blender
Middle homogenizing obtains hybrid particles B1；
(6) antibacterial agent of parts by weight is dissolved in the distilled water of 50-100 times of weight, obtains antimicrobial；By step (5) institute
It obtains hybrid particles B1 to be placed in rotating disk, and to antimicrobial is uniformly sprayed thereon, particle is placed in vacuum after the completion of sprinkling
Drying box is dry, until water content 4-8%, is then packed to get finished product cat litter C1；
The mass fraction of step (1) ethyl acetate solution is 60-80%.
The temperature of vacuum oven is 100 DEG C in step (3), and drying time is 12 hours.
Using the cat litter with pet Urine proteins warning function in application, colorimetric card can be made for convenience of use,
With rapid comparison color change.Colorimetric card can be prepared by the following method:
(1) it is taken pictures to finished product cat litter C1 and imports computer；The exceeded cat urine of Urine proteins, normal cats urine is taken to infiltrate finished product respectively
Color change result take pictures importing computer by cat litter C1, carries out color difference inspection in computer internal reference piece, passes through analysis
Obtain corresponding rgb value；Colorimetric card is made according to corresponding rgb value；
The method that wherein color difference is examined is:
(1) according to each color rgb value in Raul's colorimetric card, Raul's colorimetric card of electronic edition is generated in a computer；
(2) cat litter taken pictures and Raul's colorimetric card in kind will be needed to be placed in together, imported after being taken pictures under same environment
Computer；Whether the rgb value for then verifying the rgb value of Raul's colorimetric card in kind and standard Raul's colorimetric card of electronic edition is consistent,
When consistent, illustrate the conclusion of the rgb value no color differnece of the photo of clapped cat litter.
Embodiment 4
A kind of cat litter with pet Urine proteins warning function, is made of raw material from the following weight: modified 60 parts of Kiselgel A,
5 parts of diatomite, 5 parts of active carbon, 100 parts of Urine proteins indicator and 0.1 part of antibacterial agent；The Urine proteins indicator is bromophenol blue examination
Agent.
The bromophenol blue reagent is prepared by following methods: weighing the 0.1mol/ that bromophenol blue 0.67g is dissolved in 10ml
In L NaOH solution, add distilled water to 1000ml.
The modified Kiselgel A is prepared by following methods:
(1) silica gel particle is dipped in the ethanol solution that mass fraction is 60%, is cleaned by ultrasonic 30min, wash with distilled water postposition
35-40 DEG C of dry 30min in a vacuum drying oven, it is spare；
(2) step (1) silica obtained particle is dipped in 10-15min in ethyl acetate, it is 15% that mass fraction is dipped in after rear taking-up
Polycarbodiimide solution in 2min, after in 37 DEG C of solidification 30min obtain modified silica-gel particle.
The antibacterial agent is hexamethylene.Hexamethylene is also known as PHMB, purchased from upper
Hai Gaoju Biotechnology Co., Ltd, active principle content are 20%.
A kind of preparation method of the cat litter with pet Urine proteins warning function, comprising the following steps:
(1) it pre-processes: in 0.1mol/L citric acid solution 1000m1,0.2mol/L Na2HPO4 solution 20ml is added, adjusts pH
It is 2.1, buffer is prepared；Kiselgel A is soaked in hydrochloric acid solution after 30min and is taken out, after distilled water cleans 3-5 times
It is soaked in gained buffer and takes out after 30min, dry after 3-5 times wash with distilled water；
(2) it prepares modified silica-gel: step (1) silica obtained particle being dipped in the ethanol solution that mass fraction is 60%, ultrasound is clear
30min is washed, wash with distilled water postposition 35-40 DEG C of dry 30min in a vacuum drying oven, then silica obtained particle is dipped in second
10-15min in acetate solution, it is rear take out after be dipped in 2min in the polycarbodiimide solution that mass fraction is 15%, after in 37
DEG C solidification 30min obtain modified silica-gel particle；
(3) it prepares Urine proteins indicator: weighing bromophenol blue 0.67g and be dissolved in the 0.1mol/L NaOH solution of 10ml, add distillation
Water is to 1000ml；
(4) modified silica-gel and bromophenol blue Urine proteins indicator are weighed according to the ratio, and modified silica-gel is dipped in the instruction of bromophenol blue Urine proteins
It is stirred in agent solution, and at 50 DEG C after heating stirring 2-5h, filters to isolate silica gel particle, juxtaposition is true in a vacuum drying oven
Sky is dry, obtains first product A2；
(5) it takes first product A2 obtained by the diatomite, active carbon and step (4) of the weight to be mixed, is then placed in blender
Middle homogenizing obtains hybrid particles B2；
(6) antibacterial agent of parts by weight is dissolved in the distilled water of 50-100 times of weight, obtains antimicrobial；It will be mixed obtained by step
It closes particle B2 to be placed in rotating disk, and to antimicrobial is uniformly sprayed thereon, particle is placed in vacuum drying after the completion of sprinkling
Case is dry, until water content 4-8%, is then packed to get finished product cat litter C2.
The mass fraction of step (1) ethyl acetate solution is 60-80%.
The temperature of vacuum oven is 100 DEG C in step (3), and drying time is 12 hours.
Using the cat litter with pet Urine proteins warning function in application, colorimetric card can be made for convenience of use,
With rapid comparison color change.Colorimetric card can be prepared by the following method:
(1) it is taken pictures to finished product cat litter C2 and imports computer；The exceeded cat urine of Urine proteins, normal cats urine is taken to infiltrate finished product respectively
Color change result take pictures importing computer by cat litter C2, carries out color difference inspection in computer internal reference piece, passes through analysis
Obtain corresponding rgb value；Colorimetric card is made according to corresponding rgb value.
The method that wherein color difference is examined is:
(1) according to each color rgb value in Raul's colorimetric card, Raul's colorimetric card of electronic edition is generated in a computer；
(2) cat litter taken pictures and Raul's colorimetric card in kind will be needed to be placed in together, imported after being taken pictures under same environment
Computer；Whether the rgb value for then verifying the rgb value of Raul's colorimetric card in kind and standard Raul's colorimetric card of electronic edition is consistent,
When consistent, illustrate the conclusion of the rgb value no color differnece of the photo of clapped cat litter.
Embodiment 5
A kind of cat litter with pet Urine proteins warning function, is made of raw material from the following weight: modified 80 parts of Kiselgel A,
8 parts of diatomite, 8 parts of active carbon, 120 parts of Urine proteins indicator and 0.3 part of antibacterial agent；The Urine proteins indicator is bromophenol blue examination
Agent.
The bromophenol blue reagent is prepared by following methods: weighing the 0.1mol/ that bromophenol blue 0.67g is dissolved in 10ml
In L NaOH solution, add distilled water to 1000ml.
The modified Kiselgel A is prepared by following methods:
(1) silica gel particle is dipped in the ethanol solution that mass fraction is 60%, is cleaned by ultrasonic 30min, wash with distilled water postposition
35-40 DEG C of dry 30min in a vacuum drying oven, it is spare；
(2) step (1) silica obtained particle is dipped in 10-15min in ethyl acetate, it is 15% that mass fraction is dipped in after rear taking-up
Polycarbodiimide solution in 2min, after in 37 DEG C of solidification 30min obtain modified silica-gel particle.
The antibacterial agent is hexamethylene.Hexamethylene is also known as PHMB, purchased from upper
Hai Gaoju Biotechnology Co., Ltd, active principle content are 20%.
A kind of preparation method of the cat litter with pet Urine proteins warning function, comprising the following steps:
(1) it pre-processes: in 0.1mol/L citric acid solution 1000ml, 0.2mol/L Na2HPO4 solution 20ml is added, adjusts pH
It is 2.1, buffer is prepared；Kiselgel A is soaked in hydrochloric acid solution after 30min and is taken out, after distilled water cleans 3-5 times
It is soaked in gained buffer and takes out after 30min, dry after 3-5 times wash with distilled water；
(2) it prepares modified silica-gel: step (1) silica obtained particle being dipped in the ethanol solution that mass fraction is 60%, ultrasound is clear
30min is washed, wash with distilled water postposition 35-40 DEG C of dry 30min in a vacuum drying oven, then silica obtained particle is dipped in second
10-15min in acetate solution, it is rear take out after be dipped in 2min in the polycarbodiimide solution that mass fraction is 15%, after in 37
DEG C solidification 30min obtain modified silica-gel particle；
(3) it prepares Urine proteins indicator: weighing bromophenol blue 0.67g and be dissolved in the 0.1mol/L NaOH solution of 10ml, add distillation
Water is to 1000ml；
(4) modified silica-gel and bromophenol blue Urine proteins indicator are weighed according to the ratio, and modified silica-gel is dipped in the instruction of bromophenol blue Urine proteins
It is stirred in agent solution, and at 50 DEG C after heating stirring 2-5h, filters to isolate silica gel particle, juxtaposition is true in a vacuum drying oven
Sky is dry, obtains first product A2；
(5) it takes first product A2 obtained by the diatomite, active carbon and step (4) of the weight to be mixed, is then placed in stirring
It is homogenized in device, obtains hybrid particles B2；
(6) antibacterial agent of parts by weight is dissolved in the distilled water of 50-100 times of weight, obtains antimicrobial；It will be mixed obtained by step
It closes particle B2 to be placed in rotating disk, and to antimicrobial is uniformly sprayed thereon, particle is placed in vacuum drying after the completion of sprinkling
Case is dry, until water content 4-8%, is then packed to get finished product cat litter C2.
The mass fraction of step (1) ethyl acetate solution is 60-80%.
The temperature of vacuum oven is 100 DEG C in step (3), and drying time is 12 hours.
Using the cat litter with pet Urine proteins warning function in application, colorimetric card can be made for convenience of use,
With rapid comparison color change.Colorimetric card can be prepared by the following method:
(2) it is taken pictures to finished product cat litter C2 and imports computer；The exceeded cat urine of Urine proteins, normal cats urine is taken to infiltrate finished product respectively
Color change result take pictures importing computer by cat litter C2, carries out color difference inspection in computer internal reference piece, passes through analysis
Obtain corresponding rgb value；Colorimetric card is made according to corresponding rgb value.
The method that wherein color difference is examined is:
(1) according to each color rgb value in Raul's colorimetric card, Raul's colorimetric card of electronic edition is generated in a computer；
(2) cat litter taken pictures and Raul's colorimetric card in kind will be needed to be placed in together, imported after being taken pictures under same environment
Computer；Whether the rgb value for then verifying the rgb value of Raul's colorimetric card in kind and standard Raul's colorimetric card of electronic edition is consistent
When, when consistent, illustrate the conclusion of the rgb value no color differnece of the photo of clapped cat litter.
Embodiment 6
A kind of cat litter with pet Urine proteins warning function, is made of raw material from the following weight: modified Kiselgel A 100
Part, 10 parts of diatomite, 10 parts of active carbon, 150 parts of Urine proteins indicator and 0.5 part of antibacterial agent；The Urine proteins indicator is bromine
Phenol indigo plant reagent.
The bromophenol blue reagent is prepared by following methods: weighing the 0.1mol/ that bromophenol blue 0.67g is dissolved in 10ml
In L NaOH solution, add distilled water to 1000ml.
The modified Kiselgel A is prepared by following methods:
(1) silica gel particle is dipped in the ethanol solution that mass fraction is 60%, is cleaned by ultrasonic 30min, wash with distilled water postposition
35-40 DEG C of dry 30min in a vacuum drying oven, it is spare；
(2) step (1) silica obtained particle is dipped in 10-15min in ethyl acetate, it is 15% that mass fraction is dipped in after rear taking-up
Polycarbodiimide solution in 2min, after in 37 DEG C of solidification 30min obtain modified silica-gel particle.
The antibacterial agent is hexamethylene.Hexamethylene is also known as PHMB, purchased from upper
Hai Gaoju Biotechnology Co., Ltd, active principle content are 20%.
A kind of preparation method of the cat litter with pet Urine proteins warning function, comprising the following steps:
(1) it pre-processes: in 0.1mol/L citric acid solution 1000m1,0.2mol/L Na2HPO4 solution 20ml is added, adjusts pH
It is 2.1, buffer is prepared；Kiselgel A is soaked in hydrochloric acid solution after 30min and is taken out, after distilled water cleans 3-5 times
It is soaked in gained buffer and takes out after 30min, dry after 3-5 times wash with distilled water；
(2) it prepares modified silica-gel: step (1) silica obtained particle being dipped in the ethanol solution that mass fraction is 60%, ultrasound is clear
30min is washed, wash with distilled water postposition 35-40 DEG C of dry 30min in a vacuum drying oven, then silica obtained particle is dipped in second
10-15min in acetate solution, it is rear take out after be dipped in 2min in the polycarbodiimide solution that mass fraction is 15%, after in 37
DEG C solidification 30min obtain modified silica-gel particle；
(3) prepare Urine proteins indicator: bromophenol blue trial system weighs the 0.1mol/L NaOH that bromophenol blue 0.67g is dissolved in 10ml
In solution, add distilled water to 1000ml；
(4) modified silica-gel and bromophenol blue Urine proteins indicator are weighed according to the ratio, and modified silica-gel is dipped in the instruction of bromophenol blue Urine proteins
It is stirred in agent solution, and at 50 DEG C after heating stirring 2-5h, filters to isolate silica gel particle, juxtaposition is true in a vacuum drying oven
Sky is dry, obtains first product A2；
(5) it takes first product A2 obtained by the diatomite, active carbon and step (4) of the weight to be mixed, is then placed in blender
Middle homogenizing obtains hybrid particles B2；
(6) antibacterial agent of parts by weight is dissolved in the distilled water of 50-100 times of weight, obtains antimicrobial；It will be mixed obtained by step
It closes particle B2 to be placed in rotating disk, and to antimicrobial is uniformly sprayed thereon, particle is placed in vacuum drying after the completion of sprinkling
Case is dry, until water content 4-8%, is then packed to get finished product cat litter C2.
The mass fraction of step (1) ethyl acetate solution is 60-80%.
The temperature of vacuum oven is 100 DEG C in step (3), and drying time is 12 hours.
Using the cat litter with pet Urine proteins warning function in application, colorimetric card can be made for convenience of use,
With rapid comparison color change.Colorimetric card can be prepared by the following method:
(1) it is taken pictures to finished product cat litter C2 and imports computer；The exceeded cat urine of Urine proteins, normal cats urine is taken to infiltrate finished product respectively
Color change result take pictures importing computer by cat litter C2, carries out color difference inspection in computer internal reference piece, passes through analysis
Obtain corresponding rgb value；Colorimetric card is made according to corresponding rgb value.
The method that wherein color difference is examined is:
(1) according to each color rgb value in Raul's colorimetric card, Raul's colorimetric card of electronic edition is generated in a computer；
(2) cat litter taken pictures and Raul's colorimetric card in kind will be needed to be placed in together, imported after being taken pictures under same environment
Computer；Whether the rgb value for then verifying the rgb value of Raul's colorimetric card in kind and standard Raul's colorimetric card of electronic edition is consistent,
When consistent, illustrate the conclusion of the rgb value no color differnece of the photo of clapped cat litter.
Application test
Standard color comparison card preparation: for normal cats Urine proteins value in 15mol/dL, choosing and preparing urine protein content is 10 mol/dL, 15
Mol/dL, 20 mol/dL, 25 mol/dL urine make colorimetric card according to mode described in embodiment 1-6, wherein use clear water
Blank control the most, obtained colorimetric card color gradient trend.Wherein using biuret reagent as prepared by protein indicator
Colorimetric card it is as shown in Figure 1.When color purpling, when color is similar to 15-20 grades, illustrate Urine proteins exception.It should enhance your vigilance.
It can similarly prepare using bromophenol blue reagent as the standard color comparison card of protein indicator.
Cat litter obtained by 1-6 of the embodiment of the present invention and commercially available common silica gel cat litter are subjected to urine test experiments.Choose 35
Renal dysfunction, Urine proteins are higher than the sick cat of normal value, are randomly divided into 7 groups, S1-S6 use respectively 1-6 of the embodiment of the present invention and
S7 uses commercially available common cat litter；It chooses 35 normal cats of physical signs to be tested, is randomly divided into 7 groups, T1-T6 is used respectively
1-6 and T7 of the embodiment of the present invention use commercially available common cat litter.
Statistical performance data, as shown in table 1 below:
The cat litter of the present invention of table 1 is compared with the performance of commodity cat litter
Compare and commercially available common cat litter, it is fast that the embodiment of the present invention inhales urine speed, the time it is most short can be by 13 seconds, and commercially available cat litter is wanted
50 seconds or more again；The time of excrement dehydration hardening is set also there was only 200 seconds or so simultaneously, the 400s of far smaller than commercially available cat litter.And
The ability for absorbing peculiar smell is also relatively preferable.It may be speculated that silica gel hydrophilic modifying effect of the present invention is preferable, keep protein indicator abundant
Dip dyeing in silica gel, color reaction occurs for the urine of Urine proteins exception, and normal urine does not have color reaction, can help to dote on
Object keeper tentatively understands pet health situation, plays a warning role.
It should be noted that above-described embodiment is only the section Example for realizing preferred embodiment of the invention, Er Feiquan
Portion's embodiment.Obviously, it is based on the above embodiment of the present invention, those of ordinary skill in the art are not making creative work
Under the premise of other all embodiments obtained, should fall within the scope of the present invention.
Claims (8)
1. a kind of cat litter with pet Urine proteins warning function, which is characterized in that be made of raw material from the following weight: modified
60-100 parts of Kiselgel A, 5-10 parts of diatomite, 5-10 parts of active carbon, 100-150 parts of Urine proteins indicator and antibacterial agent 0.1-
0.5 part；The Urine proteins indicator is bromophenol blue reagent or biuret reagent.
2. according to claim 1 with the cat litter of pet Urine proteins warning function, which is characterized in that the bromophenol blue reagent
It is to be prepared by following methods: weighs bromophenol blue 0.67g and be dissolved in the 0.1mol/L NaOH solution of 10ml, add distilled water
To 1000ml.
3. according to claim 1 with the cat litter of pet Urine proteins warning function, which is characterized in that the biuret reagent
It is to be prepared by following methods: weighs 1.5g copper sulphate and 6.0g sodium potassium tartrate tetrahydrate, is dissolved, stirred with 500mL distilled water
Lower addition 300mL10% sodium hydroxide solution, is diluted with water to 1L.
4. according to claim 1 with the cat litter of pet Urine proteins warning function, which is characterized in that the modified pore silicon
Glue is prepared by following methods:
(1) silica gel particle is dipped in the ethanol solution that mass fraction is 60%, is cleaned by ultrasonic 30min, wash with distilled water postposition
35-40 DEG C of dry 30min in a vacuum drying oven, it is spare；
(2) step (1) silica obtained particle is dipped in 10-15min in ethyl acetate, it is 15% that mass fraction is dipped in after rear taking-up
Polycarbodiimide solution in 2min, after in 37 DEG C of solidification 30min obtain modified silica-gel particle.
5. according to claim 1 with the cat litter of pet Urine proteins warning function, which is characterized in that the antibacterial agent is poly-
Hexamethylene bis guanidine hydrochloride.
6. a kind of preparation method of the cat litter described in claim 1-4 any one with pet Urine proteins warning function, special
Sign is, comprising the following steps:
(1) it pre-processes: in 0.1mol/L citric acid solution 1000m1,0.2mol/L Na is added2HPO4Solution 20ml, adjustment pH are
2.1, buffer is prepared；Kiselgel A is soaked in hydrochloric acid solution after 30min and is taken out, leaching after distilled water cleans 3-5 times
It steeps and is taken out after 30min in gained buffer, dried after 3-5 times wash with distilled water；
(2) it prepares modified silica-gel: step (1) silica obtained particle being dipped in the ethanol solution that mass fraction is 60%, ultrasound is clear
30min is washed, wash with distilled water postposition 35-40 DEG C of dry 30min in a vacuum drying oven, then silica obtained particle is dipped in second
10-15min in acetate solution, it is rear take out after be dipped in 2min in the polycarbodiimide solution that mass fraction is 15%, after in 37
DEG C solidification 30min obtain modified silica-gel particle；
(3) prepare Urine proteins indicator: biuret reagent is prepared, and is weighed 1.5g copper sulphate and 6.0g sodium potassium tartrate tetrahydrate, is used 500mL
Distilled water dissolution, 300mL10% sodium hydroxide solution is added under stiring, is diluted with water to 1L；Bromophenol blue trial system, weighs bromine
Phenol indigo plant 0.67g is dissolved in the 0.1mol/L NaOH solution of 10ml, adds distilled water to 1000ml；
(4) modified silica-gel and biuret Urine proteins indicator are weighed according to the ratio, and modified silica-gel is dipped in the instruction of biuret Urine proteins
It is stirred in agent solution, and at 50 DEG C after heating stirring 2-5h, filters to isolate silica gel particle, juxtaposition is true in a vacuum drying oven
Sky is dry, obtains first product A1；Modified silica-gel and bromophenol blue Urine proteins indicator are weighed according to the ratio, and modified silica-gel is dipped in bromine
It is stirred in phenol indigo plant Urine proteins indicator solution, and filters to isolate silica gel particle after heating stirring 2-5h at 50 DEG C, be placed in
It is dried in vacuo in vacuum oven, obtains first product A2；
(5) it takes first product A1 obtained by the diatomite, active carbon and step (4) of the weight to be mixed, is then placed in blender
Middle homogenizing obtains hybrid particles B1；
(6) antibacterial agent of parts by weight is dissolved in the distilled water of 50-100 times of weight, obtains antimicrobial；By step (5) institute
It obtains hybrid particles B1 to be placed in rotating disk, and to antimicrobial is uniformly sprayed thereon, particle is placed in vacuum after the completion of sprinkling
Drying box is dry, until water content 4-8%, is then packed to get finished product cat litter C1；
(7) it takes first product A2 obtained by the diatomite, active carbon and step (4) of the weight to be mixed, is then placed in blender
Middle homogenizing obtains hybrid particles B2；
(8) antibacterial agent of parts by weight is dissolved in the distilled water of 50-100 times of weight, obtains antimicrobial；By step (7) institute
It obtains hybrid particles B2 to be placed in rotating disk, and to antimicrobial is uniformly sprayed thereon, particle is placed in vacuum after the completion of sprinkling
Drying box is dry, until water content 4-8%, is then packed to get finished product cat litter C2.
7. the preparation method of the cat litter with pet Urine proteins warning function according to claim 3, which is characterized in that step
(1) mass fraction of ethyl acetate solution is 60-80%.
8. the preparation method of the cat litter with pet Urine proteins warning function according to claim 3, which is characterized in that step
(4) temperature of vacuum oven is 100 DEG C in, and drying time is 12 hours.
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| CN112715385A (en) * | 2020-12-30 | 2021-04-30 | 青岛美今生物科技有限公司 | Preparation method of functional color-developing health indication plant deodorizing cat litter |
| CN112790113A (en) * | 2021-01-15 | 2021-05-14 | 上海毛球日记宠物用品有限公司 | Water-absorbing color-changing cat litter convenient to clean and preparation method thereof |
| CN116019021A (en) * | 2023-03-02 | 2023-04-28 | 杭州立生生物科技有限公司 | Cat litter mate with deodorizing and antibacterial functions and preparation method thereof |
| CN116953258A (en) * | 2023-08-02 | 2023-10-27 | 山东瑞达硅胶有限公司 | Pet urine protein detection particle and preparation method thereof |
| CN118177086A (en) * | 2024-03-18 | 2024-06-14 | 威海龙峰硅胶有限公司 | Preparation method of allochroic silica gel cat litter capable of judging kidney lesions |
| CN118216438A (en) * | 2024-03-29 | 2024-06-21 | 威海龙峰硅胶有限公司 | Preparation method of allochroic silica gel cat litter for renal function detection |
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