CN109287484A - Dwarf form bigleaf hydrangea method for tissue culture - Google Patents
Dwarf form bigleaf hydrangea method for tissue culture Download PDFInfo
- Publication number
- CN109287484A CN109287484A CN201811299038.8A CN201811299038A CN109287484A CN 109287484 A CN109287484 A CN 109287484A CN 201811299038 A CN201811299038 A CN 201811299038A CN 109287484 A CN109287484 A CN 109287484A
- Authority
- CN
- China
- Prior art keywords
- culture
- branch
- seedling
- hydrangea
- cultivating
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Developmental Biology & Embryology (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Cell Biology (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The present invention provides a kind of dwarf form bigleaf hydrangea method for tissue culture, comprising the following steps: 1) the dwarf form bigleaf hydrangea seedling for selecting robust growth disease-free, clip new life branch remove blade, stay part petiole, clean up spare；2) branch successively with alcoholic solution, mercuric chloride solution soaking disinfection and is rinsed well on superclean bench；3) branch is cut into segment, accesses in Primary culture base to be placed in culturing room and cultivates；4) after cultivating a period of time, axillary bud sprouting cuts in access proliferated culture medium and cultivates；5) after cultivating a period of time, base portion forms several adventitious buds, after bud grows into certain altitude, is placed in greenhouse domestication；6) after taming a period of time, seedling is cleaned up, cuts adventitious bud, carries out outside sprout-cultivating-bottle.High survival rate of the present invention, breeding cycle are short, and growth coefficient reaches 6.2, and rooting rate reaches 93%, and one step of hardening of taking root is completed, and shorten 1-2 months seedling raise periods, can be realized the generation of dwarf form bigleaf hydrangea anniversary, have a vast market foreground.
Description
Technical field
The invention belongs to plant cultivation reproduction technique field, specifically a kind of dwarf form bigleaf hydrangea method for tissue culture.
Background technique
Dwarf form bigleaf hydrangea is Saxifragaceae (Saxifragaceae) hydrangea (Hydrangea) bigleaf hydrangea
(macrophylla) a kind of cultivar general name, plant type is short and small compact, plant height 30-40cm；Internode is shorter, only 2-
5cm is the 1/5 of common bigleaf hydrangea；Inflorescence is big and beautiful, and pattern enriches, including red colour system, pink colour system, blue series, violet,
White color system and green system etc.；For basidixed corymbiform cyme, according to decoration flower number with raw mode be divided into flat flower again
Sequence and ball-type inflorescence；Shape, adnation position and the quantity of decoration flower sepal also have variation abundant.Dwarf form bigleaf hydrangea happiness half
Shade, not sun-proof, fertilizer favorite fertile, wet, well-drained soil；The natural florescence is the 5-7 month, winter fallen leaves, germination in March next year.
Dwarf form bigleaf hydrangea is excellent pot flowers material, has huge potentiality to be exploited.Since internode is short, plant type
Compact short and small, available cuttage stem section is extremely limited, therefore the breeding potential of normal cutting propagation nursery is extremely low, and reproduction technique becomes short life
The obstacle of type bigleaf hydrangea application.Currently, domestic hydrangea Plant Tissue Breeding research is mostly directed to cutting flower variety, dwarf form is big
Leaf silk ball has not been reported.
Summary of the invention
The present invention is aiming at the problems existing in the prior art, it is therefore intended that design provides a kind of dwarf form bigleaf hydrangea tissue
Cultural method is cultivated, this method growth coefficient is high, reproduction speed is fast, the hardening period is short, high survival rate.
A kind of dwarf form bigleaf hydrangea method for tissue culture of the invention, it is characterised in that the following steps are included:
1) the disease-free dwarf form bigleaf hydrangea seedling of selection robust growth, clip new life branch remove blade, stay part leaf
Handle cleans up spare；
2) branch successively with alcoholic solution, mercuric chloride solution soaking disinfection and is rinsed well on superclean bench；
3) branch is cut into segment, accesses in Primary culture base to be placed in culturing room and cultivates；
4) after cultivating a period of time, axillary bud sprouting cuts in access proliferated culture medium and cultivates；
5) after cultivating a period of time, base portion forms several adventitious buds, after bud grows into certain altitude, is placed in greenhouse and tames and dociles
Change；
6) after taming a period of time, seedling is cleaned up, cuts adventitious bud, carries out outside sprout-cultivating-bottle.
A kind of dwarf form bigleaf hydrangea method for tissue culture, it is characterised in that in step 1): mother plant for cutting is to hold
Seedling is placed in greenhouse in March by device seedling, and apply nitrogen: phosphorus: potassium ratio is the compound fertilizer 3-5g of 28:5:5, is respectively poured sooner or later daily primary
Water sprays 500-700 times of carbendazim solution every the whole strain of 7d, newborn branch is acquired after 30-40d；The newborn branch of acquisition, removal
Blade is put into abluent, in 10% sodium hypochlorite mixed solution, on magnetic stirring apparatus with 75% cotton ball soaked in alcohol wiped clean
20-30min is stirred, then spare with tap water flushing 0.5-1h.
A kind of dwarf form bigleaf hydrangea method for tissue culture, it is characterised in that in step 2): by branch with 75%
Alcohol impregnates 10-15s, after outwelling alcohol, impregnates 6-15min with 0.1% mercuric chloride solution, constantly shakes therebetween, outwell mercuric chloride
After solution, with aseptic water washing 5 times.
A kind of dwarf form bigleaf hydrangea method for tissue culture, it is characterised in that in step 3): it after branch disinfection, uses
Sterile knife is cut into segment, one section of every stipes, and upper cut is close to axillary bud, and lower cut accesses Primary culture base away from axillary bud 0.5-1cm
In；Primary culture based formulas is B5Minimal medium adds 0.5-1.0mgL-16-benzyl aminopurine, 0.1mgL-1Naphthalene second
Acid, 0.7% carragheen and 2.0% sucrose, pH are adjusted to 5.4.
A kind of dwarf form bigleaf hydrangea method for tissue culture, it is characterised in that in step 4): being cultivated in culturing room
Temperature is 25 ± 2 DEG C, light application time 12h/d, light intensity 2500Lx；Stem section culture 15-18d, axillary bud sprouting, and pumping children's spray
The edible tender branch for being wherein greater than 0.5cm is cut, accesses proliferated culture medium by item；Proliferation culture medium formula is B5Minimal medium,
Add 1.0-2.0mgL-16-benzyl aminopurine, 0.1mgL-1Methyl α-naphthyl acetate, 0.7% carragheen and 1.0%-2.0% sucrose,
PH is adjusted to 5.4.
A kind of dwarf form bigleaf hydrangea method for tissue culture, it is characterised in that in step 5): edible tender branch is increasing
Culture medium culture 35-40d is grown, base portion forms adventitious bud, and grows spray, when 75% spray length reaches 2cm or more, by tissue culture
Bottle seedling is placed on greenhouse seedbed and tames, 75% shade.
A kind of dwarf form bigleaf hydrangea method for tissue culture, it is characterised in that in step 6): outside sprout-cultivating-bottle matrix
Formula is 50% peat, 50% perlite (volume ratio), is sufficiently stirred, is distributed into 72 hole hole trays, is sprinkled profoundly water, made using preceding 7d
It is sterilized with 0.2%-0.3% potassium permanganate；After tissue-culture container seedling tames 7d, proliferation seedling is cleaned with tap water, cleans residual culture
Base cuts the spray that length is greater than 1.5cm with knife, retains top two panels leaf, remove other blades, then spray base portion exists
500-700mg·L-1Quickly dipped 5s in methyl α-naphthyl acetate is inserted into matrix, and depth is the 1/3 of spray length, is placed in full exposure and is sprayed greenhouse
In take root；Full exposure is sprayed greenhouse at 30 ± 2 DEG C of season in spring and autumn mean temperature, 35 ± 2 DEG C of summer, 15 ± 2 DEG C of winter, adjusts
Whole spray intervals time and duration make relative humidity winter be maintained at 60%-70%, other season 80%-90%, every
10d sprays 500 times of carbendazim solutions；After cultivating 35-60d, healthy and strong rooted seedling is formed.
Percentage composition involved in the present invention unless otherwise stated, refers both to weight percentage.
Dwarf form bigleaf hydrangea method for tissue culture of the invention, high survival rate, breeding cycle are short, and growth coefficient reaches
6.2, rooting rate reaches 93%, and one step of hardening of taking root is completed, and shortens 1-2 months seedling raise periods, can be realized dwarf form great Ye
The silk ball anniversary generates, and has a vast market foreground.
Specific embodiment
Embodiment 1
Object: dwarf form bigleaf hydrangea " ten thousand Hua Jing "
Container seedling is placed in greenhouse in March by step 1), and apply nitrogen: phosphorus: potassium ratio is the compound fertilizer 5g of 28:5:5, early daily
Evening respectively pours a water, sprays 600 times of carbendazim solutions every the whole strain of 7d, newborn branch is acquired after 40d；The newborn branch of acquisition,
Blade is removed, with 75% cotton ball soaked in alcohol wiped clean, abluent is put into, in 10% sodium hypochlorite mixed solution, in magnetic agitation
30min is stirred on device, then spare with tap water flushing 1h；
For step 2) on superclean bench, 75% alcohol impregnates 10s, and after outwelling alcohol, 0.1% mercuric chloride solution impregnates
During which 10min constantly shakes, spare with aseptic water washing 5 times after outwelling mercuric chloride solution；
Step 3) is cut into segment with sterile knife, and every stipes is one section, and upper cut is close to axillary bud, lower cut away from axillary bud 0.5cm,
It accesses in Primary culture base；Primary culture based formulas is B5Minimal medium adds 0.5mgL-16-benzyl aminopurine,
0.1mg·L-1Methyl α-naphthyl acetate, 0.7% carragheen and 2.0% sucrose, pH are adjusted to 5.4；
It is 25 DEG C, light application time 12h/d, light intensity 2500Lx that step 4), which cultivates room temperature,；Stem section culture 15d, axillary bud sprouting,
And pumping edible tender branch, the edible tender branch for being wherein greater than 0.5cm is cut, proliferated culture medium is accessed；Proliferation culture medium formula is
B5Minimal medium adds 1.0mgL-16-benzyl aminopurine, 0.1mgL-1Methyl α-naphthyl acetate, 0.7% carragheen and 1.0% sugarcane
Sugar, pH are adjusted to 5.4；
For step 5) edible tender branch in proliferated culture medium culture 30d, base portion forms adventitious bud, and grows spray, when 75% tender
Branch length reaches 2cm or more, and tissue-culture container seedling is placed on greenhouse seedbed and is tamed, 75% shade；
Step 6) carries out outside sprout-cultivating-bottle in May；Outside sprout-cultivating-bottle matrix formulations are 50% peat, 50% perlite (volume
Than), it is sufficiently stirred, is distributed into 72 hole hole trays, is sprinkled profoundly water using preceding 7d, sterilized using 0.2% potassium permanganate；Tissue-culture container seedling is tamed and dociled
After changing 7d, proliferation seedling is cleaned with tap water, remaining medium is cleaned, cuts the spray that length is greater than 1.5cm with knife, retain top
Two panels leaf is held, other blades are removed, then by spray base portion in 500mgL-1Quickly dipped 5s in methyl α-naphthyl acetate is inserted into matrix, depth
It is the 1/3 of spray length, is placed in full exposure and is sprayed in greenhouse and take root；Full exposure is sprayed 30 DEG C of greenhouse mean temperature, adjustment
Spray intervals time and duration, relative humidity is made to be maintained at 90%, sprays 500 times of carbendazim solutions every 10d；Culture
After 40d, healthy and strong rooted seedling is formed.
Embodiment 2
Object: dwarf form bigleaf hydrangea " flower Temari "
The newborn branch acquired after 30d in step 1) is put into abluent, in 10% sodium hypochlorite mixed solution, in magnetic force
20min is stirred on blender, then spare with tap water flushing 0.5h；Primary culture based formulas is B in step 3)5Basic culture
Base adds 1.0mgL-16-benzyl aminopurine, 0.1mgL-1Methyl α-naphthyl acetate, 0.7% carragheen and 2.0% sucrose, pH are adjusted to
5.4；Proliferation culture medium formula is B in step 4)5Minimal medium adds 1.5mgL-16-benzyl aminopurine, 0.1mgL-1
Methyl α-naphthyl acetate, 0.7% carragheen and 1.0% sucrose, pH are adjusted to 5.4；Outside sprout-cultivating-bottle is carried out in July in step 6)；By spray base
Portion is in 600mgL-1Quickly dipped 5s in methyl α-naphthyl acetate；Full exposure is sprayed 35 DEG C of greenhouse mean temperature, adjusts the spray intervals time and continues
Time makes relative humidity be maintained at 90%, sprays 500 times of carbendazim solutions every 10d；After cultivating 50d, healthy and strong take root is formed
Seedling；Other steps are the same as embodiment 1.
Embodiment 3
Object: dwarf form bigleaf hydrangea " yarn knits Miss "
The newborn branch acquired after 35d in step 1) is put into abluent, in 10% sodium hypochlorite mixed solution, in magnetic force
25min is stirred on blender, then spare with tap water flushing 0.6h；Primary culture based formulas is B in step 3)5Basic culture
Base adds 1.0mgL-16-benzyl aminopurine, 0.1mgL-1Methyl α-naphthyl acetate, 0.7% carragheen and 2.0% sucrose, pH are adjusted to
5.4；Proliferation culture medium formula is B in step 4)5Minimal medium adds 2.0mgL-16-benzyl aminopurine, 0.1mgL-1
Methyl α-naphthyl acetate, 0.7% carragheen and 1.0% sucrose, pH are adjusted to 5.4；Outside sprout-cultivating-bottle is carried out in December in step 6)；By spray base
Portion is in 700mgL-1Quickly dipped 5s in methyl α-naphthyl acetate；Full exposure is sprayed 15 DEG C of greenhouse mean temperature, adjusts the spray intervals time and continues
Time makes relative humidity be maintained at 70%, sprays 500 times of carbendazim solutions every 10d；After cultivating 55d, healthy and strong take root is formed
Seedling；Other steps are the same as embodiment 1.
Embodiment 4
Object: dwarf form bigleaf hydrangea " ten thousand Hua Jing "
Outside sprout-cultivating-bottle is carried out in December in step 6)；By spray base portion in 700mgL-1Quickly dipped 5s in methyl α-naphthyl acetate；Quan Guang
According to 15 DEG C of spraying greenhouse mean temperature, spray intervals time and duration are adjusted, relative humidity is made to be maintained at 70%, every
10d sprays 500 times of carbendazim solutions；After cultivating 55d, healthy and strong rooted seedling is formed；Other steps are the same as embodiment 1.
The dwarf form bigleaf hydrangea rooted seedling obtained using above-described embodiment 1-4 method for tissue culture, high survival rate, breeding
Speed is fast, and growth coefficient grows coefficient and reaches 6.2, and rooting rate reaches 93%, one step of hardening of taking root is completed, and shortens 1-2 months and educates
The seedling time can be realized the generation of dwarf form bigleaf hydrangea anniversary.
The foregoing is merely presently preferred embodiments of the present invention, is not intended to limit the invention, it is all in spirit of the invention and
Made any modifications, equivalent replacements, and improvements etc., is all included in the scope of protection of the present invention within principle.
Claims (7)
1. a kind of dwarf form bigleaf hydrangea method for tissue culture, it is characterised in that the following steps are included:
1) the disease-free dwarf form bigleaf hydrangea seedling of selection robust growth, clip new life branch remove blade, stay part petiole,
It cleans up spare；
2) branch successively with alcoholic solution, mercuric chloride solution soaking disinfection and is rinsed well on superclean bench；
3) branch is cut into segment, accesses in Primary culture base to be placed in culturing room and cultivates；
4) after cultivating a period of time, axillary bud sprouting cuts in access proliferated culture medium and cultivates；
5) after cultivating a period of time, base portion forms several adventitious buds, after bud grows into certain altitude, is placed in greenhouse domestication；
6) after taming a period of time, seedling is cleaned up, cuts adventitious bud, carries out outside sprout-cultivating-bottle.
2. a kind of dwarf form bigleaf hydrangea method for tissue culture as described in claim 1, it is characterised in that in step 1): adopting fringe
Maternal plant is container seedling, and seedling is placed in greenhouse in March, and apply nitrogen: phosphorus: potassium ratio is the compound fertilizer 3-5g of 28:5:5, sooner or later daily
A water is respectively poured, 500-700 times of carbendazim solution is sprayed every the whole strain of 7d, newborn branch is acquired after 30-40d；The new life of acquisition
Branch removes blade, with 75% cotton ball soaked in alcohol wiped clean, abluent is put into, in 10% sodium hypochlorite mixed solution, in magnetic force
20-30min is stirred on blender, then spare with tap water flushing 0.5-1h.
3. a kind of dwarf form bigleaf hydrangea method for tissue culture as described in claim 1, it is characterised in that in step 2): by branch
Item impregnates 10-15s with 75% alcohol, after outwelling alcohol, impregnates 6-15min with 0.1% mercuric chloride solution, constantly shakes therebetween,
After outwelling mercuric chloride solution, with aseptic water washing 5 times.
4. a kind of dwarf form bigleaf hydrangea method for tissue culture as described in claim 1, it is characterised in that in step 3): branch
After disinfection, it is cut into segment, one section of every stipes with sterile knife, upper cut is close to axillary bud, and lower cut is opened away from axillary bud 0.5-1cm, access
In dynamic culture medium；Primary culture based formulas is B5Minimal medium adds 0.5-1.0mgL-16-benzyl aminopurine, 0.1mg
L-1Methyl α-naphthyl acetate, 0.7% carragheen and 2.0% sucrose, pH are adjusted to 5.4.
5. a kind of dwarf form bigleaf hydrangea method for tissue culture as described in claim 1, it is characterised in that in step 4): culture
Cultivation temperature is 25 ± 2 DEG C, light application time 12h/d, light intensity 2500Lx in room；Stem section culture 15-18d, axillary bud sprouting, and pumping
The edible tender branch for being wherein greater than 0.5cm is cut, accesses proliferated culture medium by edible tender branch；Proliferation culture medium formula is B5Substantially
Culture medium adds 1.0-2.0mgL-16-benzyl aminopurine, 0.1mgL-1Methyl α-naphthyl acetate, 0.7% carragheen and 1.0%-
2.0% sucrose, pH are adjusted to 5.4.
6. a kind of dwarf form bigleaf hydrangea method for tissue culture as described in claim 1, it is characterised in that in step 5): children is tender
For branch in proliferated culture medium culture 35-40d, base portion forms adventitious bud, and grows spray, when 75% spray length up to 2cm with
On, tissue-culture container seedling is placed on greenhouse seedbed and is tamed, 75% shade.
7. a kind of dwarf form bigleaf hydrangea method for tissue culture as described in claim 1, it is characterised in that in step 6): outside bottle
Matrix of taking root formula is 50% peat, 50% perlite (volume ratio), is sufficiently stirred, is distributed into 72 hole hole trays, uses preceding 7d
It sprinkles profoundly water, is sterilized using 0.2%-0.3% potassium permanganate；After tissue-culture container seedling tames 7d, proliferation seedling is cleaned with tap water, is cleaned
Remaining medium cuts the spray that length is greater than 1.5cm with knife, retains top two panels leaf, other blades are removed, then by spray
Base portion is in 500-700mgL-1Quickly dipped 5s in methyl α-naphthyl acetate is inserted into matrix, and depth is the 1/3 of spray length, is placed in full exposure spray
Mist is taken root in greenhouse；Full exposure is sprayed greenhouse at 30 ± 2 DEG C of season in spring and autumn mean temperature, 35 ± 2 DEG C of summer, and winter 15 ±
2 DEG C, spray intervals time and duration are adjusted, relative humidity winter is made to be maintained at 60%-70%, other season 80%-
90%, 500 times of carbendazim solutions are sprayed every 10d；After cultivating 35-60d, healthy and strong rooted seedling is formed.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811299038.8A CN109287484A (en) | 2018-11-02 | 2018-11-02 | Dwarf form bigleaf hydrangea method for tissue culture |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811299038.8A CN109287484A (en) | 2018-11-02 | 2018-11-02 | Dwarf form bigleaf hydrangea method for tissue culture |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109287484A true CN109287484A (en) | 2019-02-01 |
Family
ID=65145431
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811299038.8A Pending CN109287484A (en) | 2018-11-02 | 2018-11-02 | Dwarf form bigleaf hydrangea method for tissue culture |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109287484A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110972947A (en) * | 2019-12-19 | 2020-04-10 | 美尚生态景观股份有限公司 | Culture medium and culture method for hydrangea-polar bear tissue culture |
| CN112273229A (en) * | 2020-10-28 | 2021-01-29 | 上海辰山植物园 | One-step seedling method for hydrangea |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ES2031588T3 (en) * | 1988-01-05 | 1992-12-16 | Leo Anne Eveleens | A METHOD FOR FLOWERING HORTENSIA PLANTS ALL YEAR ROUND. |
| CN102138526A (en) * | 2011-03-24 | 2011-08-03 | 浙江森禾种业股份有限公司 | Tissue culture method of red-leaf sacred bamboo |
| CN104304019A (en) * | 2014-10-17 | 2015-01-28 | 南京帝道农业科技有限公司 | Intermediate propagation method of hydrangea xanthoneura tissue culture |
| CN104381133A (en) * | 2014-11-14 | 2015-03-04 | 黑龙江省科学院自然与生态研究所 | Tissue culture breeding method of phlox subulata |
| CN104604735A (en) * | 2015-03-11 | 2015-05-13 | 华南农业大学 | Tissue culture and rapid propagation method for American lagerstroemia indica pink velour |
| CN104719134A (en) * | 2014-10-08 | 2015-06-24 | 江苏碧云天农林科技有限公司 | Dendrobenthemia japonica tissue culture method |
-
2018
- 2018-11-02 CN CN201811299038.8A patent/CN109287484A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ES2031588T3 (en) * | 1988-01-05 | 1992-12-16 | Leo Anne Eveleens | A METHOD FOR FLOWERING HORTENSIA PLANTS ALL YEAR ROUND. |
| CN102138526A (en) * | 2011-03-24 | 2011-08-03 | 浙江森禾种业股份有限公司 | Tissue culture method of red-leaf sacred bamboo |
| CN104719134A (en) * | 2014-10-08 | 2015-06-24 | 江苏碧云天农林科技有限公司 | Dendrobenthemia japonica tissue culture method |
| CN104304019A (en) * | 2014-10-17 | 2015-01-28 | 南京帝道农业科技有限公司 | Intermediate propagation method of hydrangea xanthoneura tissue culture |
| CN104381133A (en) * | 2014-11-14 | 2015-03-04 | 黑龙江省科学院自然与生态研究所 | Tissue culture breeding method of phlox subulata |
| CN104604735A (en) * | 2015-03-11 | 2015-05-13 | 华南农业大学 | Tissue culture and rapid propagation method for American lagerstroemia indica pink velour |
Non-Patent Citations (1)
| Title |
|---|
| 黄开勇: "《木本植物营养繁殖实用技术》", 30 September 2012, 广西科学技术出版社 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110972947A (en) * | 2019-12-19 | 2020-04-10 | 美尚生态景观股份有限公司 | Culture medium and culture method for hydrangea-polar bear tissue culture |
| CN112273229A (en) * | 2020-10-28 | 2021-01-29 | 上海辰山植物园 | One-step seedling method for hydrangea |
| CN112273229B (en) * | 2020-10-28 | 2022-02-22 | 上海辰山植物园 | One-step seedling method for hydrangea |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105494103A (en) | Method for tissue culture and rapid propagation of high-quality paphiopedilum maudiae seedlings | |
| CN102613076A (en) | Vegetative propagation method for butterfly orchid | |
| CN109287483A (en) | A kind of method of bigleaf hydrangea container seedling quickly breeding | |
| CN105706900A (en) | Sterile sowing and seedling raising method for hybrid orchid and Cymbidium tracyanum hybrid seeds | |
| CN106386489A (en) | Tissue culture and rapid propagation method of lonicera ruprechtiana | |
| CN106613988B (en) | The quasi- small method for potted of encrinite category commodity of plastotype quickly breeding difficulty cutting propagation in bottle | |
| CN106386478A (en) | Phoebe zhennan tissue culture rapid breeding method | |
| CN107667860A (en) | Tissue culture propagation method of alpinia japonica | |
| CN103430842A (en) | Quick propagation method of hybrid orchid tissue culture | |
| CN102613087B (en) | Method for culturing and breeding Correa carmen by using biological tissue | |
| CN105145363B (en) | It is a kind of to significantly improve the method that China fir tissue culture produces emergence rate | |
| CN109287484A (en) | Dwarf form bigleaf hydrangea method for tissue culture | |
| CN107079817A (en) | Pocket Lanzhou and Xinjiang kind " excellent pocket is blue " tissue culture and rapid propagation method | |
| CN104488722B (en) | A kind of quick breeding method for tissue culture of South America crutch flower | |
| CN101855995B (en) | Tissue culture propagation method of Primula mallophylla Balf.f. | |
| CN106359101A (en) | Tissue culture and rapid propagation method of ficus deltoidea | |
| CN106165648B (en) | A kind of cercis tissue culture culture medium and cultural method | |
| CN107593446A (en) | A kind of fast breeding method of the induction and regeneration of tree peony | |
| CN101743908A (en) | Tissue culture, rapid propagation and cultivation method of grevillea banksii | |
| CN108077066A (en) | A kind of caladium bicolor somatic embryogenesis pathway tissue culture and rapid propagation method | |
| CN104488721B (en) | A kind of quick breeding method for tissue culture of snowflake grass | |
| CN105454046A (en) | In-vitro rapid propagation method for lonicera praeflorens | |
| CN102884980B (en) | Quick tissue propagation method of paphiopedilum bellatulum | |
| CN109042322A (en) | A kind of rapid propagation method of gold heart A Erta Chinese ilex | |
| CN108849512A (en) | A kind of method of spun gold emperor chrysanthemum tissue culture regeneration |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |