CN109287379A - A kind of glutinous rice culture medium and its application suitable for cordyceps sinensis cultivation - Google Patents
A kind of glutinous rice culture medium and its application suitable for cordyceps sinensis cultivation Download PDFInfo
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- CN109287379A CN109287379A CN201811443312.4A CN201811443312A CN109287379A CN 109287379 A CN109287379 A CN 109287379A CN 201811443312 A CN201811443312 A CN 201811443312A CN 109287379 A CN109287379 A CN 109287379A
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- culture medium
- glutinous rice
- cordyceps sinensis
- nutrient solution
- hydrogen phosphate
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/20—Culture media, e.g. compost
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Abstract
The present invention provides a kind of glutinous rice culture medium suitable for cordyceps sinensis cultivation and its application, belongs to breed of edible fungus field, the culture medium contains glutinous rice and nutrient solution, and feed liquid mass volume ratio is 1 g:1.6mL.The nutrient solution is the 1000 mL culture solutions that glucose 20g, peptone 6g, dipotassium hydrogen phosphate 2g, magnesium sulfate 1.5g, disodium hydrogen phosphate 1.5g and urea 1.5g are dissolved in purified water composition.Nutrient solution in culture medium can be grown to cordyceps sinensis provides additional nutrition, improves the content of the nutritional ingredient of cordyceps sinensis.The present invention provides a kind of cordyceps sinensis culture medium for cultivating, and the culture matrix manufacturing is simple, easy to use, and production and processing is at low cost, and growth cycle is short, and biological transformation ratio is higher, can adapt to the factorial production and use, increase economic efficiency.
Description
Technical field
The present invention provide it is a kind of suitable for cordyceps sinensis cultivation glutinous rice culture medium and its application, belong to breed of edible fungus field.
Background technique
Cordceps militaris [Cordyceps sinensis(L .Fr) Link] it is also known as Cordyceps militaris, northern Chinese caterpillar Fungus, it is a kind of food medicine
Dual-purpose fungi, as cordyceps sinensis substitute and cultivated extensively, wide market.Artificial cultivation Cordceps militaris needs one
Fixed technology supports in the environmental factor of its growth, there is strict requirements to temperature, illumination, relative air humidity etc..
The water content of culture medium is also that can Cordceps militaris normal growth and one of an important factor for high yield.But the Cordyceps militaris of artificial cultivation is deposited
The deficiencies of mycelia growth is slow, fructification uniformity is poor, color saturation is poor, biological transformation ratio is low, product quality is different, it is studied carefully
Reason is in addition to, there are other than some problems, mainly culture medium cannot meet the nutritional need of Cordyceps militaris growth well in management.
Meanwhile there is also be formulated the problems such as complicated, production is cumbersome, at high cost for prior art cordyceps militaris plantation culture medium.
Summary of the invention
The purpose of the present invention is to provide a kind of glutinous rice culture medium suitable for cordyceps sinensis cultivation and its applications, and production is simple,
It is at low cost, nutrition needed for each growth period of Cordyceps militaris is developed can be fully met, using culture medium culture Cordyceps militaris of the present invention,
Mycelia growth is fast, biological transformation ratio is high, product quality is excellent.
To achieve the above object, the present invention adopts the following technical scheme:
It is a kind of suitable for cordyceps sinensis cultivation glutinous rice culture medium contain glutinous rice and nutrient solution, feed liquid mass volume ratio be 1 g:1.6
mL。
The nutrient solution is glucose 20g, peptone 6g, dipotassium hydrogen phosphate 2g, magnesium sulfate 1.5g, disodium hydrogen phosphate 1.5g
1000 mL culture solutions of purified water composition are dissolved in urea 1.5g.
Application of the glutinous rice culture medium in cordyceps sinensis is cultivated.
The present invention has the advantages that
Cordyceps sinensis is rare medicinal material, contains cordycepin, cordycepic acid, protein abundant, multicore glycosides, polysaccharide, alkaloid, sterol, ring
The various actives substances such as shape contracting carboxylic peptide, have antitumor, antibacterial anti-inflammatory, anti-oxidant, aging, anti-fibrosis, anti-rotation move, resist it is tired
Labor improves insulin secretion liver protection, adjusts the pharmacological actions such as immune, has wide application development prospect.The cultivation side of cordyceps sinensis
The culture medium used when method and cultivation has important influence to the growth of cordyceps sinensis.Glutinous rice contain protein, fat, carbohydrate, calcium,
Phosphorus, iron, vitamin B1, vitamin B2, niacin and starch isoreactivity ingredient, it is full of nutrition, it can grow and be provided comprehensively for cordyceps sinensis
Nutritional support.Nutrient solution in culture medium can be grown to cordyceps sinensis provides additional nutrition, improves the content of the nutritional ingredient of cordyceps sinensis.
The present invention provides a kind of cordyceps sinensis culture medium for cultivating, and the culture matrix manufacturing is simple, easy to use, produce and process at low cost, growth
Period is short, and biological transformation ratio is higher, can adapt to the factorial production and uses, increases economic efficiency.
Detailed description of the invention
Biological transformation ratio of Fig. 1 Cordyceps militaris in 4 kinds of different glutinous rice culture medium for cultivating.
The cultivation of Fig. 2 Cordyceps militaris glutinous rice.Note: A. mycelia growth period；B. sporophore growth initial stage；C. after sporophore growth
Phase.
Specific embodiment
Embodiment 1
1 materials and methods
1.1 materials and reagent
Collect strain (strain number: MF28, ITS sequence NCBI accession number: MF379054) in laboratory
The cultivation of 1.2 Cordyceps militaris glutinous rice is probed into
4 kinds of glutinous rice culture medium for cultivating formulas are devised first and carry out experiment sieving, and 4 kinds of formulas are as follows: (A), 15g glutinous rice+21mL
A nutrient solution (takes glucose 20g, peptone 6g, dipotassium hydrogen phosphate 2g, magnesium sulfate 1.5g and disodium hydrogen phosphate 1.5g to be dissolved in purifying
1000 mL culture solutions of water composition)；(B) ,+21 mL B nutrient solution of 15g glutinous rice (takes glucose 20g, peptone 6g, phosphoric acid hydrogen
Dipotassium 2g, magnesium sulfate 1.5g, disodium hydrogen phosphate 1.5g and urea 1.5g are dissolved in 1000 mL culture solutions of purified water composition)；(C),
15g glutinous rice+21mL C nutrient solution (takes glucose 20g, beef extract 6g, dipotassium hydrogen phosphate 2g, magnesium sulfate 1.5g and disodium hydrogen phosphate
1.5g is dissolved in 1000 mL culture solutions of purified water composition)；(D), 15g glutinous rice+21mL D nutrient solution (takes glucose 20g, beef
Cream 6g, dipotassium hydrogen phosphate 2g, magnesium sulfate 1.5g, disodium hydrogen phosphate 1.5g and urea 1.5g are dissolved in 1000 mL of purified water composition
Culture solution).Using Cordyceps militaris biological transformation ratio as index, the higher formula of biological transformation ratio is filtered out, and further just to cultivation
Hand over optimization.
The preparation of Cordyceps militaris strain: after cordyceps militaris link bacterial strain is activated, access is equipped with 100mL PDB fluid nutrient medium
In 250mL conical flask, 25 DEG C are placed in, 160 rpm/min shaking flask cultures 8 days.
Before experiment, first raw glutinous rice is weighed by weight, be then charged into diameter 6cm, in the glass culture bottle of high 10cm, then
A certain amount of above-mentioned nutrient solution is added, seals bottleneck using ventilative tissue culture envelope bottle film.In experiment, each different disposal group repeats 10
It is secondary, high-temperature sterilization 40min under the conditions of 121 DEG C.A certain amount of Cordyceps militaris strain is accessed after cooling in superclean bench, is sealed
Bottleneck moves into artificial cultivation incubator.Illumination is avoided between growth period of hypha, after mycelia covers with 90% glutinous rice culture medium, is adjusted
Illumination, humidity and temperature, daily illumination 12h, intensity of illumination are adjusted in 700~800 lx, air humidity control 80~90%,
Temperature is adjusted at 18~24 DEG C.During entire test, mycelia and sporophore growth situation are recorded daily.
1.3 statistical procedures
Statistical data processing and analysis are carried out using SPSS 13.0 and Desig-expert 8.0.6 software.It is examined and is divided using t
Analyse the difference between two groups.P < 0.05 is with statistical significance.
2 results and analysis
The cultivation of 2.1 Cordyceps militaris glutinous rice
Cordyceps militaris can grow mycelia and fructification in the glutinous rice culture medium for cultivating of 4 kinds of different formulations, wherein the mycelia of B formula
Long speed compared with biological transformation ratio it is other be formulated higher, biological transformation ratio can reach 71.87%.
In glutinous rice planting type, glutinous rice can provide carbon source and other microelements, and the peptone in nutrient solution is
The main source of nitrogen source, furthermore solid-liquid ratio and inoculum concentration be also be affected to bacterial strain mycelium length and fruiting body yield because
Element, so experiment is carrying out 3 factor, 3 level just to the solid-liquid ratio of nutrient solution peptone content, glutinous rice and nutrient solution, inoculum concentration
Hand over test (table 1) optimization.
From table 1 it follows that the k3 in solid-liquid ratio is maximum, the k3 in peptone content is maximum, and the k3 in inoculum concentration is most
Greatly, thus can obtain glutinous rice cultivation best mode be nutrient solution peptone content be 1%, the solid-liquid ratio of glutinous rice and nutrient solution is 1:
1.6,4.5 mL of inoculum concentration is sucrose, and under this kind of training method, mycelia covers with culture medium for cultivating and needs 3d or so, and fructification is sprouted
Sending out the time, 7d or so, the biological transformation ratio of Cordyceps militaris reach 87.45% after inoculation.It is very poor it can be found that solid-liquid ratio by analyzing
Very poor maximum, be 44.57, i.e. solid-liquid ratio is the main factor for influencing Cordyceps militaris growth, followed by peptone content, finally
It is inoculum concentration.
In variance analysis (table 2), the F value of solid-liquid ratio is up to 47.135, peptone content secondly, be 6.666, followed by
Inoculum concentration is 5.471.So the significant difference size order of 3 kinds of factors is solid-liquid ratio > peptone content > inoculum concentration, and it is straight
It is consistent to see analysis result.
1 glutinous rice of table cultivates Orthogonal Experiment and Design and visual result analysis
The variance analysis of 2 glutinous rice of table cultivation orthogonal experiments
Glutinous rice contains the multiple nutritional components such as calcium, phosphorus, iron, vitamin B1, vitamin B2, niacin and starch, is that one kind can mention
For the cultivation matrix of sufficient nutritional ingredient.Therefore, this experiment has further been probed into using novel cultivation matrix glutinous rice to Cordyceps militaris
The feasibility cultivated.Result of study discovery, though Cordyceps militaris is in 4 kinds of different formulations glutinous rice culture medium for cultivating of experimental design
Can grow mycelia and fructification, but the mycelium length of B formula compare with biological transformation ratio it is other be formulated it is higher.Experiment is further
Orthogonal optimization has been carried out to the planting type of B formula, has obtained optimal planting type are as follows: nutrient solution peptone content is 1%, glutinous rice
Solid-liquid ratio with nutrient solution is 1:1.6,4.5 mL of inoculum concentration, i.e.+24 mL B nutrient solution of 15g glutinous rice (takes glucose 20g, albumen
Peptone 10g, dipotassium hydrogen phosphate 2g, magnesium sulfate 1.5g, disodium hydrogen phosphate 1.5g and urea 1.5g are dissolved in 1000 mL of purified water composition
Culture solution) 4.5mL Cordyceps militaris strain is accessed in the culture medium for cultivating that is configured to.Under this kind of training method, mycelia is long
Full culture medium needs 3d or so, and 7d or so, biological transformation ratio reach 87.45% to fructification sprout time after inoculation.
The foregoing is merely presently preferred embodiments of the present invention, all equivalent changes done according to scope of the present invention patent with
Modification, is all covered by the present invention.
Claims (3)
1. a kind of glutinous rice culture medium suitable for cordyceps sinensis cultivation, it is characterised in that: the culture medium contains glutinous rice and nutrient solution, material
Liquid mass volume ratio is 1 g:1.6 mL.
2. a kind of glutinous rice culture medium suitable for cordyceps sinensis cultivation according to claim 1, it is characterised in that: the nutrient solution
Purifying is dissolved in for glucose 20g, peptone 6g, dipotassium hydrogen phosphate 2g, magnesium sulfate 1.5g, disodium hydrogen phosphate 1.5g and urea 1.5g
1000 mL culture solutions of water composition.
3. application of the glutinous rice culture medium as described in claim 1 in cordyceps sinensis is cultivated.
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Citations (5)
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|---|---|---|---|---|
| CN101940131A (en) * | 2010-10-09 | 2011-01-12 | 镇江市食用菌研究所 | Method for culturing cordyceps militaris by using drink bottle |
| CN104860756A (en) * | 2015-05-08 | 2015-08-26 | 南通大学 | Cordyceps militaris fermentation liquid culture medium, preparation method thereof and application thereof |
| CN104885787A (en) * | 2015-06-17 | 2015-09-09 | 桂林丰润莱生物科技有限公司 | Planting method for cordyceps militaris sporocarp high in active matter content |
| CN106748240A (en) * | 2016-12-31 | 2017-05-31 | 新昌县迪斯曼科技有限公司 | It is the method that culture medium cultivates Chinese caterpillar fungus to solidify nutrient solution with water-loss reducer |
| CN107484547A (en) * | 2017-08-02 | 2017-12-19 | 南阳理工学院 | A kind of method that medium culture Cordyceps militaris is made using blueberry leaf as major ingredient |
-
2018
- 2018-11-29 CN CN201811443312.4A patent/CN109287379A/en active Pending
Patent Citations (5)
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| CN101940131A (en) * | 2010-10-09 | 2011-01-12 | 镇江市食用菌研究所 | Method for culturing cordyceps militaris by using drink bottle |
| CN104860756A (en) * | 2015-05-08 | 2015-08-26 | 南通大学 | Cordyceps militaris fermentation liquid culture medium, preparation method thereof and application thereof |
| CN104885787A (en) * | 2015-06-17 | 2015-09-09 | 桂林丰润莱生物科技有限公司 | Planting method for cordyceps militaris sporocarp high in active matter content |
| CN106748240A (en) * | 2016-12-31 | 2017-05-31 | 新昌县迪斯曼科技有限公司 | It is the method that culture medium cultivates Chinese caterpillar fungus to solidify nutrient solution with water-loss reducer |
| CN107484547A (en) * | 2017-08-02 | 2017-12-19 | 南阳理工学院 | A kind of method that medium culture Cordyceps militaris is made using blueberry leaf as major ingredient |
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| 张晓封等: "《中国虫草研究 历史·资源·科学》", 31 January 2008, 陕西科学技术出版社 * |
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