CN109280626A - A kind of microbial compound inoculant formula and its production - Google Patents
A kind of microbial compound inoculant formula and its production Download PDFInfo
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- CN109280626A CN109280626A CN201710598429.9A CN201710598429A CN109280626A CN 109280626 A CN109280626 A CN 109280626A CN 201710598429 A CN201710598429 A CN 201710598429A CN 109280626 A CN109280626 A CN 109280626A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05C—NITROGENOUS FERTILISERS
- C05C11/00—Other nitrogenous fertilisers
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/80—Soil conditioners
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
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Abstract
The present invention relates to agricultural technology fields, especially a kind of formula of microbial compound inoculant: 30-40 parts of bacillus, 60-90 parts of carrier, 20-35 parts of Soluble phosphorus bacterial strain, 15-30 parts of lactobacillus plantarum, 30-50 parts of nutritional agents, 70-100 parts of culture solution, the present invention also provides a kind of production methods of microbial compound inoculant formula, comprising the following steps: culture medium prepares, the culture of bacterium solution and viable bacteria are inoculated with.The present invention is by being added bacillus, Soluble phosphorus bacterial strain, lactobacillus plantarum and nutritional agents, the phosphorus that Soluble phosphorus bacterial strain is absorbed to being difficult to be converted to plant by the phosphorus that crops absorb in soil, absorption of the crops to phosphorus is improved in this way, and nutritional agents is for nutriment needed for providing crop growth, lactobacillus plantarum and bacillus purify for reducing the pH value in soil and to water quality, so that the environment of the growth of crops is more suitable for, reduce the cost of the purchase of chemical fertilizer in this way, and also improves environmental-protecting performance.
Description
Technical field
The present invention relates to agricultural technology field more particularly to a kind of microbial compound inoculant formula and its productions.
Background technique
Currently, agricultural fertilizer mainly includes inorganic fertilizer, organic fertilizer and bio-feritlizer.Inorganic fertilizer is agricultural production
It makes a great contribution, but chronic administration chemical fertilizer, also brings some negative effects to the mankind, be mainly manifested in environment
Pollution, a large amount of chemical fertilizer of applying are that lake, river, reservoir water body eutrophic extent is caused to get worse, and groundwater azotate concentration is anxious
Increase severely high, seriously affects the life of the people, also, as people's awareness of environmental protection continues to increase, green food and life
The development of state agricultural starts to consider suitably to reduce fertilizer amount, increases the dosage of organic fertilizer and bio-feritlizer, in addition, with
Continuous improvement of people's living standards, the requirement to the quality and quantity of agricultural product is also continuously improved, and cultivated area is not
Disconnected to reduce, the production of chemical fertilizer and application cost are also very high, and increasing with applying quantity of chemical fertilizer, and insoluble phosphorus also increases in soil
It is more, it is unfavorable for crops and absorbs nutrient, the fertilizer efficiency and utilization rate of chemical fertilizer can reduce, studies at home and abroad show that, plant rhizosphere is deposited
In a large amount of beneficial microbes, it is indispensable active constituent of increasing soil fertility that microorganism, which is organism living in soil,
In the prior art, both at home and abroad in relation to the Inoculant between crops rhizosphere based on single rhizobium, there is not yet collection
Close Rhizobium strains, Soluble phosphorus bacterial strain and the crops rhizosphere microbial compound inoculant for secreting plant hormone bacterial strain, crops rhizosphere
Space state is still in terms of microbial compound inoculant, be badly in need of crops rhizosphere microbial compound inoculant single variety and
The low practical problem of growgh promoting effects.
Summary of the invention
The purpose of the present invention is to solve fertilizer cost height existing in the prior art, fertilizer efficiency is low, safety is poor, non-
Regenerated resources consumption is big, the feature of environmental protection is poor and the disadvantage of microbial inoculant single variety and growth-promoting effect difference, and one proposed
Kind microbial compound inoculant formula and its production.
To achieve the goals above, present invention employs following technical solutions:
A kind of microbial compound inoculant formula is designed, the formula of the microbial compound inoculant by weight is as follows:
30-40 parts of bacillus
60-90 parts of carrier
20-35 parts of Soluble phosphorus bacterial strain
15-30 parts of lactobacillus plantarum
30-50 parts of nutritional agents
70-100 parts of culture solution.
Preferably, the nutritional agents is by arbuscular mycorrhizal fungi and glucose group at and arbuscular mycorrhizal fungi: glucose
Weight ratio is 5:2.
Preferably, the bacillus is bacillus megaterium, bacillus licheniformis, bacillusmusilaginosiengineering, side spore bud
Spore bacillus or azotobacter chroococcum.
Preferably, the carrier is peat.
Preferably, the culture solution is made of peptone, yeast extract, sodium chloride, aqueous solution and agar, and albumen
Peptone: yeast extract: sodium chloride: aqueous solution: the weight ratio of fine jade is 5:3:5:50:1.
The present invention also provides a kind of production methods of microbial compound inoculant formula, comprising the following steps:
S1, culture medium prepare, and after the completion of 70-100 parts of culture solution preparations, pour into culture medium, spare；
The culture of S2, bacterium solution, after the culture medium in S1 is ready to complete, respectively by 30-40 parts of bacillus, phosphorus-solubilizing bacteria
20-35 parts of strain, 15-30 parts of lactobacillus plantarum and 30-50 parts of nutritional agents are seeded in fermentation in culture medium and generate liquid viable bacteria system
Agent；
Liquid active bacteria formulation is seeded in carrier 60- after the completion of the preparation of the liquid active bacteria formulation of S2 by S3, viable bacteria inoculation
On 90 parts, absorption cultivates and wheat rhizosphere microbial compound inoculant is made.
Preferably, the temperature of the culture medium is 25-30 DEG C.
Preferably, the culture medium is LB culture medium.
A kind of microbial compound inoculant formula proposed by the present invention and its production, beneficial effect are: the present invention passes through
It is added bacillus, Soluble phosphorus bacterial strain, lactobacillus plantarum and nutritional agents, Soluble phosphorus bacterial strain in soil to being difficult to be absorbed by crops
Phosphorus is converted to plant convenient for the phosphorus absorbed, in this way absorption of the raising crops to phosphorus, and nutritional agents is for providing crop growth
Required nutriment, lactobacillus plantarum and bacillus purify for reducing the pH value in soil and to water quality, so as to agriculture
The environment of the growth of crop is more suitable for reducing the cost of the purchase of chemical fertilizer in this way, and also improve environmental-protecting performance, subtracting
The consumption of few resource, so that Inoculant variety diversity and raising promote the effect of row crop growth.
Specific embodiment
Technical scheme in the embodiment of the invention is clearly and completely described, it is clear that described embodiment is only
It is only a part of the embodiment of the present invention, instead of all the embodiments.
Embodiment one
A kind of microbial compound inoculant formula, the formula of microbial compound inoculant by weight are as follows:
30 parts of bacillus
60 parts of carrier
20 parts of Soluble phosphorus bacterial strain
15 parts of lactobacillus plantarum
30 parts of nutritional agents
70 parts of culture solution.
Nutritional agents is by arbuscular mycorrhizal fungi and glucose group at and arbuscular mycorrhizal fungi: the weight ratio of glucose is 5:2.
Bacillus is bacillus megaterium, bacillus licheniformis, bacillusmusilaginosiengineering, bacillus laterosporus or circle
Brown nitrogen-fixing bacteria.
Carrier is peat.
Culture solution is made of peptone, yeast extract, sodium chloride, aqueous solution and agar, and peptone: yeast extracts
Object: sodium chloride: aqueous solution: the weight ratio of fine jade is 5:3:5:50:1.
The present invention also provides a kind of production methods of microbial compound inoculant formula, comprising the following steps:
S1, culture medium prepare, and after the completion of 70 parts of culture solution preparations, pour into culture medium, spare, and culture medium is LB culture
Base；
The culture of S2, bacterium solution, after the culture medium in S1 is ready to complete, respectively by 30 parts of bacillus, Soluble phosphorus bacterial strain 20
Part, 15 parts of lactobacillus plantarum and 30 parts of nutritional agents are seeded in fermentation in culture medium and generate liquid active bacteria formulation；
Liquid active bacteria formulation is seeded in carrier 60 after the completion of the preparation of the liquid active bacteria formulation of S2 by S3, viable bacteria inoculation
On part, wheat rhizosphere microbial compound inoculant is cultivated and is made in absorption, and the temperature of culture medium is 25 DEG C.
Embodiment two
A kind of microbial compound inoculant formula, the formula of microbial compound inoculant by weight are as follows:
33 parts of bacillus
68 parts of carrier
22 parts of Soluble phosphorus bacterial strain
18 parts of lactobacillus plantarum
37 parts of nutritional agents
82 parts of culture solution.
Nutritional agents is by arbuscular mycorrhizal fungi and glucose group at and arbuscular mycorrhizal fungi: the weight ratio of glucose is 5:2.
Bacillus is bacillus megaterium, bacillus licheniformis, bacillusmusilaginosiengineering, bacillus laterosporus or circle
Brown nitrogen-fixing bacteria.
Carrier is peat.
Culture solution is made of peptone, yeast extract, sodium chloride, aqueous solution and agar, and peptone: yeast extracts
Object: sodium chloride: aqueous solution: the weight ratio of fine jade is 5:3:5:50:1.
The present invention also provides a kind of production methods of microbial compound inoculant formula, comprising the following steps:
S1, culture medium prepare, and after the completion of 82 parts of culture solution preparations, pour into culture medium, spare, and culture medium is LB culture
Base；
The culture of S2, bacterium solution, after the culture medium in S1 is ready to complete, respectively by 33 parts of bacillus, Soluble phosphorus bacterial strain 22
Part, 18 parts of lactobacillus plantarum and 37 parts of nutritional agents are seeded in fermentation in culture medium and generate liquid active bacteria formulation；
Liquid active bacteria formulation is seeded in carrier 68 after the completion of the preparation of the liquid active bacteria formulation of S2 by S3, viable bacteria inoculation
On part, wheat rhizosphere microbial compound inoculant is cultivated and is made in absorption, and the temperature of culture medium is 26.5 DEG C.
Embodiment three
A kind of microbial compound inoculant formula, the formula of microbial compound inoculant by weight are as follows:
37 parts of bacillus
76 parts of carrier
28 parts of Soluble phosphorus bacterial strain
24 parts of lactobacillus plantarum
42 parts of nutritional agents
90 parts of culture solution.
Nutritional agents is by arbuscular mycorrhizal fungi and glucose group at and arbuscular mycorrhizal fungi: the weight ratio of glucose is 5:2.
Bacillus is bacillus megaterium, bacillus licheniformis, bacillusmusilaginosiengineering, bacillus laterosporus or circle
Brown nitrogen-fixing bacteria.
Carrier is peat.
Culture solution is made of peptone, yeast extract, sodium chloride, aqueous solution and agar, and peptone: yeast extracts
Object: sodium chloride: aqueous solution: the weight ratio of fine jade is 5:3:5:50:1.
The present invention also provides a kind of production methods of microbial compound inoculant formula, comprising the following steps:
S1, culture medium prepare, and after the completion of 90 parts of culture solution preparations, pour into culture medium, spare, and culture medium is LB culture
Base；
The culture of S2, bacterium solution, after the culture medium in S1 is ready to complete, respectively by 37 parts of bacillus, Soluble phosphorus bacterial strain 28
Part, 24 parts of lactobacillus plantarum and 42 parts of nutritional agents are seeded in fermentation in culture medium and generate liquid active bacteria formulation；
Liquid active bacteria formulation is seeded in carrier 76 after the completion of the preparation of the liquid active bacteria formulation of S2 by S3, viable bacteria inoculation
On part, wheat rhizosphere microbial compound inoculant is cultivated and is made in absorption, and the temperature of culture medium is 28 DEG C.
Example IV
A kind of microbial compound inoculant formula, the formula of microbial compound inoculant by weight are as follows:
40 parts of bacillus
90 parts of carrier
35 parts of Soluble phosphorus bacterial strain
30 parts of lactobacillus plantarum
50 parts of nutritional agents
100 parts of culture solution.
Nutritional agents is by arbuscular mycorrhizal fungi and glucose group at and arbuscular mycorrhizal fungi: the weight ratio of glucose is 5:2.
Bacillus is bacillus megaterium, bacillus licheniformis, bacillusmusilaginosiengineering, bacillus laterosporus or circle
Brown nitrogen-fixing bacteria.
Carrier is peat.
Culture solution is made of peptone, yeast extract, sodium chloride, aqueous solution and agar, and peptone: yeast extracts
Object: sodium chloride: aqueous solution: the weight ratio of fine jade is 5:3:5:50:1.
The present invention also provides a kind of production methods of microbial compound inoculant formula, comprising the following steps:
S1, culture medium prepare, and after the completion of 100 parts of culture solution preparations, pour into culture medium, spare, and culture medium is LB culture
Base；
The culture of S2, bacterium solution, after the culture medium in S1 is ready to complete, respectively by 40 parts of bacillus, Soluble phosphorus bacterial strain 35
Part, 30 parts of lactobacillus plantarum and 50 parts of nutritional agents are seeded in fermentation in culture medium and generate liquid active bacteria formulation；
Liquid active bacteria formulation is seeded in carrier 90 after the completion of the preparation of the liquid active bacteria formulation of S2 by S3, viable bacteria inoculation
On part, wheat rhizosphere microbial compound inoculant is cultivated and is made in absorption, and the temperature of culture medium is 30 DEG C.
The foregoing is only a preferred embodiment of the present invention, but protection scope of the present invention be not limited to
This, anyone skilled in the art in the technical scope disclosed by the present invention, according to the technique and scheme of the present invention
And its inventive concept is subject to equivalent substitution or change, should be covered by the protection scope of the present invention.
Claims (8)
1. a kind of microbial compound inoculant formula, which is characterized in that the formula of the microbial compound inoculant by weight is such as
Under:
30-40 parts of bacillus
60-90 parts of carrier
20-35 parts of Soluble phosphorus bacterial strain
15-30 parts of lactobacillus plantarum
30-50 parts of nutritional agents
70-100 parts of culture solution.
2. a kind of microbial compound inoculant formula according to claim 1, which is characterized in that the nutritional agents is by clump branch
Mycorrhizal fungi and glucose group are at and arbuscular mycorrhizal fungi: the weight ratio of glucose is 5:2.
3. a kind of microbial compound inoculant formula according to claim 1, which is characterized in that the bacillus is huge
Bacterium anthracoides, bacillus licheniformis, bacillusmusilaginosiengineering, bacillus laterosporus or azotobacter chroococcum.
4. a kind of microbial compound inoculant formula according to claim 1, which is characterized in that the carrier is peat.
5. a kind of microbial compound inoculant formula formula according to claim 1, which is characterized in that the culture solution is by egg
White peptone, yeast extract, sodium chloride, aqueous solution and agar composition, and peptone: yeast extract: sodium chloride: aqueous solution: fine jade
Weight ratio be 5:3:5:50:1.
6. a kind of production method of microbial compound inoculant formula according to claim 1, which is characterized in that including with
Lower step:
S1, culture medium prepare, and after the completion of 70-100 parts of culture solution preparations, pour into culture medium, spare；
The culture of S2, bacterium solution, after the culture medium in S1 is ready to complete, respectively by 30-40 parts of bacillus, Soluble phosphorus bacterial strain 20-
35 parts, 15-30 parts of lactobacillus plantarum and 30-50 parts of nutritional agents are seeded in fermentation in culture medium and generate liquid active bacteria formulation；
Liquid active bacteria formulation is seeded in 60-90 parts of carrier after the completion of the preparation of the liquid active bacteria formulation of S2 by S3, viable bacteria inoculation
On, absorption cultivates and wheat rhizosphere microbial compound inoculant is made.
7. a kind of production method of microbial compound inoculant formula according to claim 6, which is characterized in that the training
The temperature for supporting base is 25-30 DEG C.
8. a kind of production method of microbial compound inoculant formula according to claim 6, which is characterized in that the training
Supporting base is LB culture medium.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201710598429.9A CN109280626A (en) | 2017-07-21 | 2017-07-21 | A kind of microbial compound inoculant formula and its production |
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| CN201710598429.9A CN109280626A (en) | 2017-07-21 | 2017-07-21 | A kind of microbial compound inoculant formula and its production |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113373958A (en) * | 2021-07-21 | 2021-09-10 | 苏州金螳螂园林绿化景观有限公司 | Microbial curing liquid for sandy side slope and sandy side slope treatment method |
| CN113695382A (en) * | 2020-05-23 | 2021-11-26 | 中国环境科学研究院 | Microorganism soil layer dispersion mixing inoculation method |
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| US20080248953A1 (en) * | 2005-11-17 | 2008-10-09 | Mcgill University | Use of Bacteriocins For Promoting Plant Growth and Disease Resistance |
| CN101294141A (en) * | 2007-04-28 | 2008-10-29 | 上海四季生物科技有限公司 | A set of living body microorganism preparations for preparing composite microorganism fertilizer and preparation method thereof |
| CN103740616A (en) * | 2013-12-30 | 2014-04-23 | 邵素英 | Biological compound fertilizer |
| CN106883033A (en) * | 2017-03-18 | 2017-06-23 | 广州聚禅现代农业研究院有限公司 | A kind of composite microbiological fertilizer and preparation method thereof |
-
2017
- 2017-07-21 CN CN201710598429.9A patent/CN109280626A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20080248953A1 (en) * | 2005-11-17 | 2008-10-09 | Mcgill University | Use of Bacteriocins For Promoting Plant Growth and Disease Resistance |
| CN101294141A (en) * | 2007-04-28 | 2008-10-29 | 上海四季生物科技有限公司 | A set of living body microorganism preparations for preparing composite microorganism fertilizer and preparation method thereof |
| CN103740616A (en) * | 2013-12-30 | 2014-04-23 | 邵素英 | Biological compound fertilizer |
| CN106883033A (en) * | 2017-03-18 | 2017-06-23 | 广州聚禅现代农业研究院有限公司 | A kind of composite microbiological fertilizer and preparation method thereof |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113695382A (en) * | 2020-05-23 | 2021-11-26 | 中国环境科学研究院 | Microorganism soil layer dispersion mixing inoculation method |
| CN113695382B (en) * | 2020-05-23 | 2023-08-15 | 中国环境科学研究院 | Microbial soil layer dispersion mixing inoculation method |
| CN113373958A (en) * | 2021-07-21 | 2021-09-10 | 苏州金螳螂园林绿化景观有限公司 | Microbial curing liquid for sandy side slope and sandy side slope treatment method |
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