CN109275743B - Anoectochilus roxburghii health-care green tea and preparation method thereof - Google Patents

Anoectochilus roxburghii health-care green tea and preparation method thereof Download PDF

Info

Publication number
CN109275743B
CN109275743B CN201811242148.0A CN201811242148A CN109275743B CN 109275743 B CN109275743 B CN 109275743B CN 201811242148 A CN201811242148 A CN 201811242148A CN 109275743 B CN109275743 B CN 109275743B
Authority
CN
China
Prior art keywords
green tea
tea
health
anoectochilus roxburghii
parts
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN201811242148.0A
Other languages
Chinese (zh)
Other versions
CN109275743A (en
Inventor
吕鑑泉
张恒铭
邱志安
邹林江
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Jiaying University
Original Assignee
Jiaying University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Jiaying University filed Critical Jiaying University
Priority to CN201811242148.0A priority Critical patent/CN109275743B/en
Publication of CN109275743A publication Critical patent/CN109275743A/en
Application granted granted Critical
Publication of CN109275743B publication Critical patent/CN109275743B/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23F—COFFEE; TEA; THEIR SUBSTITUTES; MANUFACTURE, PREPARATION, OR INFUSION THEREOF
    • A23F3/00—Tea; Tea substitutes; Preparations thereof
    • A23F3/06—Treating tea before extraction; Preparations produced thereby
    • A23F3/14—Tea preparations, e.g. using additives
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23F—COFFEE; TEA; THEIR SUBSTITUTES; MANUFACTURE, PREPARATION, OR INFUSION THEREOF
    • A23F3/00—Tea; Tea substitutes; Preparations thereof
    • A23F3/06—Treating tea before extraction; Preparations produced thereby

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Medicines Containing Plant Substances (AREA)
  • Non-Alcoholic Beverages (AREA)

Abstract

The invention discloses anoectochilus roxburghii health-care green tea and a preparation method thereof, and aims to provide health-care green tea which is prepared by scientifically mixing green tea with rare Chinese medicinal materials, namely anoectochilus roxburghii and liquorice, so that the functions of soothing the liver and strengthening the spleen, tonifying qi and nourishing blood of the tea are enhanced, the tea is mild in nature, and the health-care green tea has the effects of promoting the production of body fluid to quench thirst, soothing the liver and expelling toxin, refreshing and restoring consciousness, strengthening the spleen and stomach, and prolonging life and beautifying. The anoectochilus roxburghii health-care green tea comprises the following components in parts by mass: 100-250 parts of green tea, 1-35 parts of anoectochilus roxburghii and 10-60 parts of liquorice.

Description

Anoectochilus roxburghii health-care green tea and preparation method thereof
Technical Field
The invention relates to health-care tea and a preparation method thereof, in particular to anoectochilus roxburghii health-care green tea and a preparation method thereof.
Background
Green tea is one of the main tea types in china, and is made by fermenting new leaves or buds of tea trees. The green tea retains the nutrients of tea polyphenol, catechin, chlorophyll, caffeine, amino acids, vitamins and the like in fresh leaves, has special effects of preventing aging, cancer, sterilization, inflammation and the like, and is beyond the reach of other teas. However, green tea lacks numerous functional active ingredients, limited by the variety of tea. In recent years, with the improvement of living standard and health care consciousness of people, health care green tea is emphasized, and the development of new formula and new function becomes a trend of health care green tea.
Anoectochilus roxburghii, also known as Kaliptae herba, Hypericum japonicum etc., is a rare medicinal plant of Kaliptae genus of Orchidaceae family. In folks, the anoectochilus roxburghii has sweet and mild nature and taste, is called as "Yaowang" and "Shencao" in essence, is rich in important active substances such as flavone, polysaccharide, triterpenoid organic acid, alkaloid and the like, and has pharmacological effects of protecting liver, resisting inflammation, reducing blood sugar, reducing blood fat, reducing blood pressure and the like. In recent years, the health care function of anoectochilus formosanus is widely hot and popular among people, and is a high-grade food material for hotels, restaurants and families.
At present, there are few domestic related documents about Anoectochilus roxburghii tea, and there are two types. The first type is that anoectochilus formosanus tea is made by anoectochilus formosanus pretreated by fermentation and the like, and the anoectochilus formosanus tea mostly adopts stem leaves after roots are removed as anoectochilus formosanus raw materials; however, the anoectochilus formosanus tea of the type does not fully utilize the roots of the anoectochilus formosanus rich in important active substances, so that raw materials are wasted, the important active substances are converted in the fermentation process, and the health-care function of the anoectochilus formosanus is lost. The second type is to directly prepare anoectochilus formosanus tea from non-fermented or otherwise pre-treated anoectochilus formosanus, which retains the original flavor of anoectochilus formosanus but generally has less desirable taste and flavor, and in particular, contains more pungent taste such as aconitine in anoectochilus formosanus.
Disclosure of Invention
One of the purposes of the invention is to provide anoectochilus roxburghii health-care green tea, which adopts the scientific compatibility of green tea and rare Chinese medicinal materials anoectochilus roxburghii and liquorice, so that the functions of soothing the liver and strengthening the spleen, tonifying qi and nourishing blood of the tea are enhanced, the tea is mild in property, and the tea has the effects of promoting the production of body fluid to quench thirst, soothing the liver and expelling toxin, refreshing the brain, strengthening the spleen and stomach, prolonging life and beautifying, and eliminates the pungent taste and toxic and side effects of aconitine and the like.
In order to achieve the above object, the technical solution provided by the present invention is as follows: the anoectochilus roxburghii health-care green tea comprises the following components in parts by mass: 100-250 parts of green tea, 1-35 parts of anoectochilus roxburghii and 10-60 parts of liquorice.
Preferably, the composition comprises the following components in parts by mass: 150-200 parts of green tea, 10-25 parts of anoectochilus roxburghii and 20-50 parts of liquorice.
Preferably, the green tea is one or more of Lushan Yunwu tea, Enshi Yulu tea, Longyan slanglian green tea, Yingshan Yunwu green tea, Xinyang Maojian green tea and Wufeng Maojian green tea.
The compatibility principle of the invention is as follows: green tea is cool in nature and is used for preventing heatstroke, and anoectochilus formosanus also belongs to a cool traditional Chinese medicinal material; the direct matching of the anoectochilus formosanus and the green tea inevitably causes the excessive coolness of the tea, and the drinking crowd is affected; the invention removes the toxicity of components such as aconitine in anoectochilus formosanus by using the liquorice, thereby realizing the health-care function of the anoectochilus formosanus green tea.
The second purpose of the invention is to provide a preparation method of the anoectochilus roxburghii health-care green tea.
In order to achieve the above object, the technical solution provided by the present invention is as follows: a preparation method of anoectochilus roxburghii health-care green tea comprises the steps of respectively baking green tea, anoectochilus roxburghii and liquorice till the water content is 6-10%, crushing, mixing according to a ratio, and sterilizing.
Preferably, the baking temperature is 80-100 ℃.
Preferably, the crushed materials are sieved by a 30-40 mesh sieve, and preferably a 40 mesh sieve.
Preferably, the sterilization condition is ultraviolet lamp sterilization for 0.5-1 hour.
Preferably, the sterilization also comprises bagging.
The invention has the beneficial effects that:
(1) the health-care green tea provided by the invention adopts the scientific compatibility of the green tea and rare Chinese medicinal materials anoectochilus roxburghii and liquorice, so that the functions of soothing the liver, strengthening the spleen, tonifying qi and nourishing blood of the tea are enhanced, the prepared health-care green tea is mild in nature, has the effects of promoting the production of body fluid to quench thirst, soothing the liver, expelling toxin, refreshing, restoring consciousness, strengthening the spleen and stomach, prolonging life and beautifying, and eliminates the pungent taste and toxic and side effects of aconitine and the like.
(2) The anoectochilus roxburghii health-care green tea provided by the invention contains various components with health-care functions of soothing liver, strengthening spleen, tonifying qi and nourishing blood, such as polysaccharide, amino acid and flavone, so that the anoectochilus roxburghii health-care green tea has an obvious health-care function.
(3) The anoectochilus roxburghii health-care green tea provided by the invention is a beverage with good flavor and good taste by adjusting the raw material formula.
Drawings
FIG. 1 is a standard graph of flavones;
FIG. 2 is a standard graph of polysaccharides;
FIG. 3 is a plot of amino acids.
Detailed Description
The following claims are hereby incorporated into the detailed description of the invention, with the understanding that the present disclosure is to be considered as a full and non-limiting example, and any limited number of modifications that fall within the spirit and scope of the claims are intended to be included therein.
Example 1
1) Purifying and drying: selecting anoectochilus formosanus and liquorice which are not damaged by worms and have obvious heavy dirt, cleaning with clear water, and removing soil, dirt, microorganisms and mixed bacteria attached to the surfaces; drying Lushan mist green tea, herba Anoectochili Roxburghii and Glycyrrhrizae radix at 80 deg.C respectively until the water content is 10%.
2) Crushing: pulverizing dried Lushan mist tea, herba Anoectochili Roxburghii and Glycyrrhrizae radix respectively, and sieving with 40 mesh sieve to obtain Lushan mist tea, herba Anoectochili Roxburghii and Glycyrrhrizae radix crushed material.
3) Bagging and sterilizing: respectively putting 150 parts of Lushan mist tea, 10 parts of anoectochilus formosanus and 15 parts of crushed liquorice into non-woven fabric bags, sealing the non-woven fabric bags, and sterilizing the non-woven fabric bags for 0.5 hour by using an ultraviolet lamp to obtain the anoectochilus formosanus health-care green tea.
The anoectochilus roxburghii health-care green tea is brewed with 150ml of boiled water for 5-10 min and can be drunk.
The anoectochilus formosanus health-care green tea water prepared by the embodiment keeps the faint scent and the taste of Lushan mist tea water, and meanwhile, the slightly sweet aftertaste of the tea water is increased. The active ingredients in the tea water are measured according to the following detection method, and the result is as follows: compared with Lushan mist green tea water with the same quality, each 150ml of Anoectochilus roxburghii health green tea water obtained in the embodiment respectively increases 20 mug of polysaccharide, 45 mug of amino acid and 50 mug of flavone, and aconitine components are not detected.
Example 2
1) Purifying and drying: selecting anoectochilus formosanus and liquorice which are not damaged by worms and have obvious heavy dirt, cleaning with clear water, and removing soil, dirt, microorganisms and mixed bacteria attached to the surfaces; baking at 100 deg.C until the water content of Enshi Yulu green tea, Anoectochilus formosanus and radix Glycyrrhizae is 6%.
2) Crushing: respectively crushing the dried Enshengyuo tea, anoectochilus formosanus and liquorice, and sieving the crushed materials with a 40-mesh sieve to obtain the Enshengyuo tea, the anoectochilus formosanus and the crushed liquorice.
3) Bagging and sterilizing: respectively putting 150 parts of Enshenyulu tea, 15 parts of anoectochilus formosanus and 20 parts of crushed liquorice into non-woven fabric bags, sealing the non-woven fabric bags with each bag being 3 g, and sterilizing the non-woven fabric bags for 1 hour by using an ultraviolet lamp.
The anoectochilus roxburghii health-care green tea is brewed with 150ml of boiled water for 5-10 min and can be drunk. The anoectochilus roxburghii health-care green tea water prepared by the embodiment keeps the faint scent and the taste of the emyulu tea water, and meanwhile, the slightly sweet aftertaste of the tea water is increased. The active ingredients in the tea water are measured according to the following detection method, and the result is as follows: compared with the same mass of the Enshi Yulu tea, 32 mug of polysaccharide, 57 mug of amino acid and 72 mug of flavone are respectively added to each 150ml of the anoectochilus roxburghii health-care green tea prepared by the embodiment, and aconitine components are not detected.
Example 3
1) Purifying and drying: selecting anoectochilus formosanus and liquorice which are not damaged by worms and have obvious heavy dirt, cleaning with clear water, and removing soil, dirt, microorganisms and mixed bacteria attached to the surfaces; baking at 90 deg.C until the water content of Longyan obliquely backed green tea, Anoectochilus roxburghii and Glycyrrhrizae radix is 8%.
2) Crushing: respectively crushing the dried Longyan backthern tea, the Anoectochilus roxburghii and the liquorice, and sieving by a 40-mesh sieve to obtain crushed Longyan backthern tea, Anoectochilus roxburghii and liquorice.
3) Bagging and sterilizing: respectively putting 200 parts of Longyan sloping-back tea, 20 parts of anoectochilus formosanus and 20 parts of crushed liquorice into non-woven fabric bags, sealing the non-woven fabric bags, and sterilizing the non-woven fabric bags for 0.8 hour by using an ultraviolet lamp.
The anoectochilus roxburghii health-care green tea is brewed with 150ml of boiled water for 5-10 min and can be drunk. The anoectochilus roxburghii health-care green tea water prepared by the embodiment keeps the faint scent and the taste of the Longyan back-inclined tea water, and meanwhile, the slightly sweet aftertaste of the tea water is increased. The active ingredients in the tea water are measured according to the following detection method, and the result is as follows: compared with Longyan backthern tea with the same quality, the anoectochilus roxburghii health-care green tea prepared by the embodiment in each 150ml of the anoectochilus roxburghii health-care green tea is added with 31 mug of polysaccharide, 61 mug of amino acid and 73 mug of flavone respectively, and aconitine components are not detected.
Example 4
1) Purifying and drying: selecting anoectochilus formosanus and liquorice which are not damaged by worms and have obvious heavy dirt, cleaning with clear water, and removing soil, dirt, microorganisms and mixed bacteria attached to the surfaces; baking at 95 deg.C until the water content of the green tea, herba Anoectochili Roxburghii and Glycyrrhrizae radix is 7%.
2) Crushing: respectively crushing the dried Yingshan Yunwu tea, Anoectochilus roxburghii and liquorice, and sieving by a 40-mesh sieve to obtain crushed Yingshan Yunwu tea, Anoectochilus roxburghii and liquorice.
3) Bagging and sterilizing: respectively putting 150 parts of Yingshan Yunwu tea, 10 parts of Anoectochilus roxburghii and 18 parts of crushed liquorice into non-woven fabric bags, sealing the non-woven fabric bags with each bag being 3 g, and sterilizing the non-woven fabric bags for 0.9 hour by using an ultraviolet lamp.
The anoectochilus roxburghii health-care green tea is brewed with 150ml of boiled water for 5-10 min and can be drunk. The anoectochilus roxburghii health-care green tea water prepared by the embodiment keeps the faint scent and the taste of the Yingshan Yunwu tea water, and meanwhile, the slightly sweet aftertaste of the tea water is increased. The active ingredients in the tea water are measured according to the following detection method, and the result is as follows: compared with the Yingshan Yunwu tea with the same quality, the Anoectochilus roxburghii health-care green tea prepared by the embodiment of every 150ml has the added 18 mug of polysaccharide, 22 mug of amino acid and 46 mug of flavone, and aconitine components are not detected.
Example 5
1) Purifying and drying: selecting anoectochilus formosanus and liquorice which are not damaged by worms and have obvious heavy dirt, cleaning with clear water, and removing soil, dirt, microorganisms and mixed bacteria attached to the surfaces; baking at 92 deg.C until the water content of green tea, herba Anoectochili Roxburghii and Glycyrrhrizae radix is 9%.
2) Crushing: respectively crushing the dried Xinyang tippy tea, anoectochilus roxburghii and liquorice, and sieving the crushed materials with a 40-mesh sieve to obtain the Xinyang tippy tea, the anoectochilus roxburghii and the crushed liquorice.
3) Bagging and sterilizing: respectively putting 150 parts of Xinyang Maojian tea, 12 parts of anoectochilus formosanus and 15 parts of crushed liquorice into non-woven fabric bags, sealing the non-woven fabric bags with each bag being 3 g, and sterilizing the non-woven fabric bags for 0.6 hour by using an ultraviolet lamp.
The anoectochilus roxburghii health-care green tea is brewed with 150ml of boiled water and can be drunk. The anoectochilus roxburghii health-care green tea water prepared by the embodiment keeps the faint scent and the taste of Xinyang Maojian tea water, and meanwhile, the slightly sweet aftertaste of the tea water is increased. The active ingredients in the tea water are measured according to the following detection method, and the result is as follows: compared with Xinyang Maojian tea water with the same quality, 22 mug of polysaccharide, 35 mug of amino acid and 42 mug of flavone are respectively added in each 150ml of the anoectochilus roxburghii health-care green tea water prepared by the embodiment, and aconitine components are not detected.
Example 6
1) Purifying and drying: selecting anoectochilus formosanus and liquorice which are not damaged by worms and have obvious heavy dirt, cleaning with clear water, and removing soil, dirt, microorganisms and mixed bacteria attached to the surfaces; baking at 98 deg.C until water content of WUFENGMAOJIAN green tea, herba Anoectochili Roxburghii and Glycyrrhrizae radix is 8%.
2) Crushing: respectively crushing the dried Maojian tea, anoectochilus formosanus and liquorice, and sieving the crushed materials with a 40-mesh sieve to obtain the Maojian tea, the anoectochilus formosanus and the crushed liquorice.
3) Bagging and sterilizing: respectively putting 200 parts of Maojian tea, 20 parts of anoectochilus formosanus and 25 parts of crushed liquorice into non-woven fabric bags, sealing the non-woven fabric bags with 3 g of each bag, and sterilizing the non-woven fabric bags for 0.7 hour by using an ultraviolet lamp.
The anoectochilus roxburghii health-care green tea is brewed with 200ml of boiled water for 5-10 min and can be drunk. The anoectochilus roxburghii health-care green tea water prepared by the embodiment keeps the faint scent and the taste of Maojian tea water and increases the slightly sweet aftertaste of the tea water. The active ingredients in the tea water are measured according to the following detection method, and the result is as follows: compared with the same-quality Wufeng Maojian tea water, the Anoectochilus roxburghii health-care green tea water prepared by the embodiment of the invention has the advantages that 18 mu g of polysaccharide, 26 mu g of amino acid and 35 mu g of flavone are respectively added in every 200ml of Anoectochilus roxburghii health-care green tea water, and aconitine components are not detected.
The above-mentioned detection methods for polysaccharides, amino acids, flavones and aconitine in the anoectochilus formosanus green tea prepared in examples 1 to 6 were as follows:
preparing tea water: soaking 5 bags (3 g each) of the same anoectochilus roxburghii health-care green tea in boiled water with a fixed volume (Vml) for 5-10 min, taking a proper volume of cooled tea water, and respectively measuring signals (recorded as a) of polysaccharide, amino acid, flavone and aconitine according to the method; soaking another 5 bags (each 3 g) of the same green tea in fixed volume (Vml) of boiled water for 5-10 min, and taking appropriate volume of cooled tea water (V)1ml), signals (recorded as b) for determination of polysaccharides, amino acids, flavones and aconitine were taken according to the methods described above, respectively.
The active substances added in the anoectochilus roxburghii health-care green tea water are calculated according to the following formula: component (μ g) ═ XV/5V1And X is the content of the corresponding signal (a-b).
Method for detecting flavone
1. Standard curve of flavone
Accurately weighing 10mg of quercetin, dissolving in 100mL of water solution, and preparing into 0.1mg/mL of quercetin standard solution as stock solution.
Measuring standard solutions of 0.00mL, 0.40mL, 0.60mL, 0.80mL, 1.00mL, 1.20mL, 1.60mL and 2.00mL, respectively placing the standard solutions in a 10mL colorimetric tube, sequentially and respectively adding 2mL of 30% ethanol and 0.2mL of 5% sodium nitrite solution, shaking up, standing for 5min, adding 0.2mL of 10% aluminum nitrate solution, shaking up, standing for 6min, adding 2mL of 4% sodium hydroxide, fixing the volume to a scale with 30% ethanol, shaking up, standing for 10-15min, measuring the Absorbance (Absorbance/Abs) at 331nm, measuring in parallel for three times, taking the Absorbance as a horizontal coordinate and the quercetin mass concentration as a vertical coordinate, drawing a standard curve, and referring to fig. 1 in detail.
2. Measurement of flavone content
Measuring 1.00-3.00mL of tea water into a 10mL colorimetric tube, sequentially and respectively adding 2mL of 30% ethanol and 0.2mL of 5% sodium nitrite solution, shaking up, standing for 5min, adding 0.2mL of 10% aluminum nitrate solution, shaking up, standing for 6min, adding 2mL of 4% sodium hydroxide, fixing the volume to a scale with 30% ethanol, shaking up, standing for 10-15min, measuring the absorbance at 331nm, carrying out parallel measurement for three times, and calculating the flavone content from a standard curve by using the average value.
Second, detection method of polysaccharide
1. Drawing of standard curve
Preparing a glucose standard solution: precisely weighing 250mg of 105 ℃ dry constant weight glucose, placing the glucose into a 250mL measuring flask, dissolving and fixing the volume to 250mL, shaking up to obtain 1.0g/L standard stock solution, and refrigerating for later use.
1mL, 2mL, 3mL, 4mL, and 5mL of the above standard solution were removed, and 10mL of distilled water was used as a blank. Adding 1.0mL of 5% phenol solution into each test tube, mixing uniformly, rapidly adding 5.0mL of concentrated sulfuric acid, shaking for 5min, standing for 30min, measuring absorbance at the maximum absorption wavelength of 490nm, performing parallel measurement for three times, drawing a standard curve by taking the absorbance A as an ordinate and the glucose mass concentration C as an ordinate, and obtaining the detailed graph shown in FIG. 2.
2. Determination of polysaccharide content
Weighing 1.00-3.00mL of tea water in a 25mL colorimetric tube, diluting the tea water to 10mL by using distilled water, adding 1mL of 5% phenol solution, uniformly mixing, quickly adding 5.0mL of concentrated sulfuric acid, shaking for 5min, standing for 30min, measuring absorbance at the maximum absorption wavelength of 490nm, carrying out parallel measurement for three times, and calculating the polysaccharide content from a standard curve by using the average value.
Method for detecting amino acid
1. Drawing of standard curve
The preparation of standard solution comprises drying L-tyrosine standard at 100 deg.C to constant weight, precisely weighing 10.2mg, placing in a beaker, adding 10mL of 0.02mol/L sodium hydroxide, dissolving completely, draining to volumetric flask with glass rod, adding distilled water to constant volume, transferring into a reagent bottle to obtain 0.1mg/mL-1The standard solution of L-tyrosine (1).
Accurately sucking 0.0 mL, 0.2mL, 0.4mL, 0.6 mL, 0.8 mL and 1.0mL of L-tyrosine standard mother liquor, respectively placing in a 10mL colorimetric tube, and adding water to supplement the volume to 1.6 mL. And then adding 0.4mL of phosphate buffer solution, fully mixing, adding 0.4mL of ninhydrin reagent, fully mixing, carrying out color reaction in a boiling water bath at 90-100 ℃, cooling, and measuring the absorbance at 573 nm. The absorbance is used as the ordinate, the tyrosine mass concentration is used as the ordinate, and a standard curve is drawn, which is detailed in figure 3.
2. Determination of amino acid content
Weighing 1.00-3.00mL of tea water into a 10mL colorimetric tube, adding 0.2mL of 2% ninhydrin solution and 0.4mL of phosphate buffer solution with the pH value of 6.8, shaking up, placing in a water bath kettle, heating for 15min, taking out, rapidly cooling to room temperature with cold water, adding distilled water to dilute to a scale, shaking up, carrying out color development reaction in a boiling water bath at 90-100 ℃, measuring the absorbance of a sample extracting solution by taking a color development agent solution as a blank control without adding a sample solution or an L-tyrosine standard solution, and carrying out parallel measurement for 3 times, wherein the average value calculates the amino acid content from a standard curve.
Detection method of aconitine
The method is carried out according to the method for measuring the content of aconitine in monkshood mother root of the version in the Chinese pharmacopoeia 2015:
octadecylsilane chemically bonded silica is used as a filler in chromatographic conditions and system applicability tests; gradient elution is carried out according to the specification by taking acetonitrile-tetrahydrofuran (25:15) as a mobile phase A and taking 0.lmol/L ammonium acetate solution (0.5 ml of glacial acetic acid is added in every 1000 ml) as a mobile phase B; the detection wavelength was 235 nm. The number of theoretical plates is not less than 2000 calculated according to the peak of mesaconitine.
Preparation of control solutions: taking appropriate amount of aconitine reference substance, hypaconitine reference substance, and mesaconine reference substance, precisely weighing, and adding isopropanol-tri-formol (1:1) mixed solution to obtain each lml of mixed solution containing 50mg of aconitine, 0.15mg of hypaconitine and mesaconine respectively.
Preparation of a test solution: sieving the powder with a third sieve, weighing 2g, placing in a conical flask with a plug, adding 3ml of ammonia test solution, precisely adding 50ml of isopropanol-ethyl acetate (1:1) mixed solution, weighing, performing ultrasonic treatment (power 300W, frequency 40 kHZ; water temperature below 25 ℃) for 30 minutes, cooling, weighing again, supplementing the reduced weight with the isopropanol-ethyl acetate (1:1) mixed solution, shaking regularly, and filtering. Precisely taking 25ml of subsequent filtrate, recovering the solvent to dryness under reduced pressure below 40ftC, precisely adding 3ml of isopropanol-tri-peaches (1:1) mixed solution into the residue, dissolving, filtering, and taking the subsequent filtrate to obtain the final product.

Claims (8)

1. The anoectochilus roxburghii health-care green tea is characterized by comprising the following components in parts by mass: 100-250 parts of green tea, 1-35 parts of anoectochilus roxburghii and 10-60 parts of liquorice.
2. The anoectochilus roxburghii health-care green tea as claimed in claim 1 is characterized by comprising the following components in parts by mass: 150-200 parts of green tea, 10-25 parts of anoectochilus roxburghii and 20-50 parts of liquorice.
3. The anoectochilus formosanus health-care green tea as claimed in claim 1 or 2, wherein the green tea is one or more of Lushan Yunwu tea, Enshi Yulu tea, Longyan slangli back green tea, Yingshan Yunwu green tea, Xinyang Maojian green tea and Wufeng Maojian green tea.
4. A preparation method of the anoectochilus roxburghii health-care green tea as claimed in claim 1, wherein the green tea, the anoectochilus roxburghii and the liquorice are baked until the water content is 6-10%, crushed, mixed according to a ratio, and sterilized.
5. The method for preparing anoectochilus roxburghii health-care green tea according to claim 4, wherein the baking temperature is 80-100 ℃.
6. The method for preparing anoectochilus roxburghii health-care green tea according to claim 4 or 5, wherein the anoectochilus roxburghii health-care green tea is ground and sieved by a 30-40-mesh sieve.
7. The method for preparing anoectochilus roxburghii health-care green tea according to claim 4 or 5, wherein the sterilization condition is ultraviolet lamp sterilization for 0.5-1 hour.
8. The method for preparing anoectochilus roxburghii health-care green tea as claimed in claim 4 or 5, further comprising bagging before sterilization.
CN201811242148.0A 2018-10-24 2018-10-24 Anoectochilus roxburghii health-care green tea and preparation method thereof Active CN109275743B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201811242148.0A CN109275743B (en) 2018-10-24 2018-10-24 Anoectochilus roxburghii health-care green tea and preparation method thereof

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201811242148.0A CN109275743B (en) 2018-10-24 2018-10-24 Anoectochilus roxburghii health-care green tea and preparation method thereof

Publications (2)

Publication Number Publication Date
CN109275743A CN109275743A (en) 2019-01-29
CN109275743B true CN109275743B (en) 2021-08-10

Family

ID=65177238

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201811242148.0A Active CN109275743B (en) 2018-10-24 2018-10-24 Anoectochilus roxburghii health-care green tea and preparation method thereof

Country Status (1)

Country Link
CN (1) CN109275743B (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109864327A (en) * 2019-03-20 2019-06-11 福建绿色黄金生物科技有限公司 One grows tea and roxburgh anoectochilus terminal bud composition, preparation method and applications

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103815076A (en) * 2014-03-23 2014-05-28 董玉平 Anoectochilus roxburghii health maintenance white tea and preparation method thereof
CN103976053A (en) * 2014-01-12 2014-08-13 永安桃源春生态茶业有限公司 Anoectochilus roxburghii black tea
CN104146117A (en) * 2013-12-30 2014-11-19 儒兰（福建）生物科技有限公司 Anoectochilus Formosanus Hayata tea powder and preparation method thereof

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104146117A (en) * 2013-12-30 2014-11-19 儒兰（福建）生物科技有限公司 Anoectochilus Formosanus Hayata tea powder and preparation method thereof
CN103976053A (en) * 2014-01-12 2014-08-13 永安桃源春生态茶业有限公司 Anoectochilus roxburghii black tea
CN103815076A (en) * 2014-03-23 2014-05-28 董玉平 Anoectochilus roxburghii health maintenance white tea and preparation method thereof

Also Published As

Publication number Publication date
CN109275743A (en) 2019-01-29

Similar Documents

Publication Publication Date Title
KR100989869B1 (en) Composition for dissolving hangover containing fermented bean sprouts juice
KR20110080470A (en) Health food composition for immune enhancement and antioxidant containing extract of red ginseng and fermented extract of herbs
CN108186690A (en) A kind of Chinese medicine processing method
KR101462570B1 (en) Puer tea and preparation method thereof
CN104161154B (en) Utilize a kind of method that Herba Potentillae Discoloris rhizome prepares hypoglycemic teabag
CN101433324A (en) Ganoderma lucidum composite instant granule tea and preparation method thereof
CN107129892A (en) A kind of beauty treatment grape wine
CN109275743B (en) Anoectochilus roxburghii health-care green tea and preparation method thereof
CN103409282B (en) Preparation method of health-care green tea sparkling wine
CN106166123B (en) Skin care compositions, lotion with antiallergic itching-relieving efficacies and preparation method thereof
CN102696803B (en) Jujube tea and method for preparing beverage of same
CN115644286A (en) Anoectochilus roxburghii health-preserving tea tablet and preparation method thereof
CN109136058A (en) A kind of lithocarpus litseifolius health-care vinegar and preparation method thereof
CN109620874A (en) The application of Rosa roxburghii Tratt general flavone
KR101627312B1 (en) Process for saengmaeksan drink
CN106148136A (en) A kind of brewing method of Agaricus blazei Murrill health promoting wine
CN104719534A (en) Anti-aging tea bag and preparation method thereof
CN105941792A (en) Tea-flavored sweets having effects of eliminating foul breath and refreshing mind and preparation method of tea-flavored sweets
CN105030731A (en) Preparation method of microcapsule with protection effect on alcoholic liver injury
CN105379888A (en) Application of osmanthus tea with optimal antioxidation synergistic effect in preparation of health care products or food for regulating blood sugar level
CN105746786B (en) A kind of preparation method of low-caffeine, high dissolubility sugar wing Eurya plant tea
CN111494450B (en) Preparation method of isatis root indigo-momordica grosvenori seed tea
CN103931814A (en) Black tea capable of supporting healthy energy and conserving strength and preparation method thereof
CN107446765A (en) A kind of moistening lung tea wine and preparation method thereof
CN109294852A (en) A kind of tealeaves dinner party with singsong girls in attendance and preparation method thereof

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
GR01 Patent grant
GR01 Patent grant