CN109266549A - A kind of immunocyte culture bracket - Google Patents
A kind of immunocyte culture bracket Download PDFInfo
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- CN109266549A CN109266549A CN201811247500.XA CN201811247500A CN109266549A CN 109266549 A CN109266549 A CN 109266549A CN 201811247500 A CN201811247500 A CN 201811247500A CN 109266549 A CN109266549 A CN 109266549A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
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Abstract
The present invention provides a kind of immunocyte culture bracket, including several fibrous framework layers being stacked, the fibrous framework layer includes that several radiuses spaced from inside to outside are sequentially increased and concentric circular fiber silk and several radial strip-shaped fibrous silks, several strip-shaped fibrous silks are from the center of circle of several circular fiber silks to external radiation, one end that several strip-shaped fibrous silks are located at the center of circle of several circular fiber silks is fixedly connected, and the fibrous framework layer is woven by several strip-shaped fibrous silks and several circular fiber silks.The present invention has the advantages of increasing cell culturing surfaces product, more conducively cell is grown.
Description
Technical field
The present invention relates to a kind of immunocyte culture brackets.
Background technique
Immunocyte, which refers to, participates in immune response or cell relevant to immune response.It is thin including lymphocyte, dendron shape
Born of the same parents, Monocytes/Macrophages, granulocyte, mast cell etc..Immunocyte can there are many, various immunocytes in human body
Serve as important role.Immunocyte is commonly called as leucocyte, including congenital lymphocyte, various phagocytes etc. and can identify
Antigen, the lymphocyte for generating specific immune response etc., conventional immunocyte culture apparatus, using such as culture plate, bottle, ware
Deng, it is that cell is cultivated in two-dimensional surface, the planar growth mode of cell and the stereoscopic three-dimensional growth pattern in practical living body
Difference may be directed at confirmation knowledge cell, the biochemistry of tissue, pathological characteristics and have an adverse effect, and cell is in plane culture
What is grown is slow, and the cell or cell secreta of single culture harvest are few, it is difficult to meet the needs of mass cell culture, it can
To research and develop the dimensional culture bracket for utilizing immunocyte culture.
Therefore, it is badly in need of a kind of increase cell culturing surfaces product, more conducively the immunocyte culture bracket of cell growth.
Summary of the invention
In order to solve the problems, such as that existing immunocyte cultivates slow growth in two-dimensional surface, the object of the present invention is to provide
The advantages of a kind of immunocyte culture bracket has and increases cell culturing surfaces product, and more conducively cell is grown.
The present invention provides the following technical solutions:
A kind of immunocyte culture bracket, including several fibrous framework layers being stacked, the fibrous framework layer includes
Several radiuses spaced from inside to outside are sequentially increased and concentric circular fiber silk and several radial strip-shaped fibrous silks,
For several strip-shaped fibrous silks from the center of circle of several circular fiber silks to external radiation, several strip-shaped fibrous silks are located at several circular fiber silks
The one end in the center of circle be fixedly connected, the fibrous framework layer is woven by several strip-shaped fibrous silks and several circular fiber silks.
Several circular fiber silks and several strip-shaped fibrous silks are woven into the fibrous framework layer, several fibres
Dimensional scaffold layer stackup is placed, and cell culturing surfaces product is increased under conditions of not increasing spatial volume, and more conducively cell is grown,
Cell Proliferation amount, can be with the increase of tens times of upper tubers of stemona, it can be achieved that the large-scale culture of cell compared with two-dimensional surface culture.
Preferably, several circular fiber silks and several strip-shaped fibrous silks are in the same plane.
Preferably, the circular fiber silk is equipped with several spaced first through hole, sets on the strip-shaped fibrous silk
There are several spaced second through-holes.It is raw that the setting of the first through hole and second through-hole can further increase cell
Long surface area, cell can also be grown in the setting of the first through hole and second through-hole.
Preferably, several first through hole are uniformly distributed on the circular fiber silk, and several second through-holes are in the bar shaped
It is uniformly distributed on fiber filament.
Preferably, the distance between adjacent circular fiber silk is 200-400 μm.
Preferably, the diameter of the circular fiber silk and the strip-shaped fibrous silk is 300-400 μm.
Preferably, the internal diameter of the first through hole and second through-hole is 150-200 μm.
Preferably, the quantity of the fibrous framework layer is no less than 2.
The beneficial effects of the present invention are: several circular fiber silks and several strip-shaped fibrous silks are woven into the fibre
Dimensional scaffold layer, several fibrous framework layer stackups are placed, and cell culture table is increased under conditions of not increasing spatial volume
The growth of area, more conducively cell, cell Proliferation amount, can be with the increases of tens times of upper tubers of stemona compared with two-dimensional surface culture, can be real
The large-scale culture of existing cell.
Detailed description of the invention
Attached drawing is used to provide further understanding of the present invention, and constitutes part of specification, with reality of the invention
It applies example to be used to explain the present invention together, not be construed as limiting the invention.In the accompanying drawings:
Fig. 1 is schematic structural view of the invention；
Fig. 2 is fibrous framework schematic diagram of a layer structure of the present invention；
In the figure, it is marked as 1, fibrous framework layer；2, circular fiber silk；21, first through hole；3, strip-shaped fibrous silk；31, second
Through-hole.
Specific embodiment
A specific embodiment of the invention is described with reference to the accompanying drawing.
As shown in Figs. 1-2, a kind of immunocyte culture bracket, the fibrous framework layer being stacked including 3, the fiber
Shelf layer includes that several radiuses spaced from inside to outside are sequentially increased and concentric circular fiber silk and several radial
Strip-shaped fibrous silk, the distance between adjacent circular fiber silk are 200-400 μm, and several strip-shaped fibrous silks are from several circular fibers
The center of circle of silk is to external radiation, and one end that several strip-shaped fibrous silks are located at the center of circle of several circular fiber silks is fixedly connected, Ruo Ganhuan
Shape fiber filament and several strip-shaped fibrous silks are in the same plane, and the diameter of the circular fiber silk and the strip-shaped fibrous silk is
300-400 μm, the fibrous framework layer is woven by several strip-shaped fibrous silks and several circular fiber silks.Several annulars
Fiber filament 2 and several strip-shaped fibrous silks 3 are woven into the fibrous framework layer 1, and several fibrous frameworks 1 stack layer by layer
It sets, cell culturing surfaces product is increased under conditions of not increasing spatial volume, more conducively cell is grown, cell Proliferation amount and two
Dimensional plane culture is compared, can be with the increase of tens times of upper tubers of stemona.
The circular fiber silk is equipped with several spaced first through hole, and several first through hole are in the circular fiber
It is uniformly distributed on silk, the strip-shaped fibrous silk is equipped with several spaced second through-holes, and several second through-holes are in the item
It is uniformly distributed on shape fiber filament, the internal diameter of the first through hole and second through-hole is 150-200 μm.Laser boring can be used
Circular fiber silk 2 and strip-shaped fibrous silk 3 are punched, cell can also be in the first through hole 21 and second through-hole 31
Setting growth, the setting of the first through hole 21 and second through-hole 31 can further increase cell grow surface
Product.
Working method of the present invention: the fibrous framework layer that immunocyte can weave in circular fiber silk 2 and strip-shaped fibrous silk 3
1 surface and the growth of gap inner surface, immunocyte can also be grown in first through hole 21 and the second through-hole 31.
The foregoing is only a preferred embodiment of the present invention, is not intended to restrict the invention, although referring to aforementioned reality
Applying example, invention is explained in detail, for those skilled in the art, still can be to aforementioned each implementation
Technical solution documented by example is modified or equivalent replacement of some of the technical features.It is all in essence of the invention
Within mind and principle, any modification, equivalent replacement, improvement and so on be should all be included in the protection scope of the present invention.
Claims (8)
1. a kind of immunocyte culture bracket, which is characterized in that including several fibrous framework layers being stacked, the fiber branch
Rack-layer includes that several radiuses spaced from inside to outside are sequentially increased and concentric circular fiber silk and radial several
Shape fiber filament, from the center of circle of several circular fiber silks to external radiation, several strip-shaped fibrous silks are located at several several strip-shaped fibrous silks
The one end in the center of circle of circular fiber silk is fixedly connected, and the fibrous framework layer is by several strip-shaped fibrous silks and several circular fiber silks
It weaves.
2. a kind of immunocyte culture bracket according to claim 1, which is characterized in that several circular fiber silks and several
Strip-shaped fibrous silk is in the same plane.
3. a kind of immunocyte culture bracket according to claim 1, which is characterized in that the circular fiber silk is equipped with
Several spaced first through hole, the strip-shaped fibrous silk are equipped with several spaced second through-holes.
4. a kind of immunocyte culture bracket according to claim 3, which is characterized in that several first through hole are in the ring
It is uniformly distributed on shape fiber filament, several second through-holes are uniformly distributed on the strip-shaped fibrous silk.
5. a kind of immunocyte culture bracket according to claim 1, which is characterized in that between adjacent circular fiber silk
Distance be 200-400 μm.
6. a kind of immunocyte culture bracket according to claim 1, which is characterized in that the circular fiber silk and described
The diameter of strip-shaped fibrous silk is 300-400 μm.
7. a kind of immunocyte culture bracket according to claim 3, which is characterized in that the first through hole and described
The internal diameter of two through-holes is 150-200 μm.
8. a kind of immunocyte culture bracket according to claim 1, which is characterized in that the quantity of the fibrous framework layer
No less than 2.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811247500.XA CN109266549A (en) | 2018-10-25 | 2018-10-25 | A kind of immunocyte culture bracket |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811247500.XA CN109266549A (en) | 2018-10-25 | 2018-10-25 | A kind of immunocyte culture bracket |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109266549A true CN109266549A (en) | 2019-01-25 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201811247500.XA Pending CN109266549A (en) | 2018-10-25 | 2018-10-25 | A kind of immunocyte culture bracket |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB2605009A (en) * | 2021-03-14 | 2022-09-21 | Copner Biotech Ltd | Bio-scaffold |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101050418A (en) * | 2007-03-20 | 2007-10-10 | 东南大学 | Bioreactor of artificial liver |
| CN101781815A (en) * | 2010-02-03 | 2010-07-21 | 东华大学 | Preparation method of porous fiber with controllable degradation rate for tissue engineering scaffold |
| CN201587944U (en) * | 2009-08-04 | 2010-09-22 | 上海圣纳堡生物科技开发有限公司 | Stereoscopic cell culture bracket |
| CN105087381A (en) * | 2015-08-28 | 2015-11-25 | 深圳爱生再生医学科技有限公司 | 3D immune cell culture scaffold and preparation method thereof |
| CN205803499U (en) * | 2016-07-08 | 2016-12-14 | 广州洁特生物过滤股份有限公司 | Cell culturing bracket |
| US20180016551A1 (en) * | 2016-07-12 | 2018-01-18 | Tallinn University Of Technology | Self-aligned fibrous scaffolds for automechanoinduction of cell cultures |
-
2018
- 2018-10-25 CN CN201811247500.XA patent/CN109266549A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101050418A (en) * | 2007-03-20 | 2007-10-10 | 东南大学 | Bioreactor of artificial liver |
| CN201587944U (en) * | 2009-08-04 | 2010-09-22 | 上海圣纳堡生物科技开发有限公司 | Stereoscopic cell culture bracket |
| CN101781815A (en) * | 2010-02-03 | 2010-07-21 | 东华大学 | Preparation method of porous fiber with controllable degradation rate for tissue engineering scaffold |
| CN105087381A (en) * | 2015-08-28 | 2015-11-25 | 深圳爱生再生医学科技有限公司 | 3D immune cell culture scaffold and preparation method thereof |
| CN205803499U (en) * | 2016-07-08 | 2016-12-14 | 广州洁特生物过滤股份有限公司 | Cell culturing bracket |
| US20180016551A1 (en) * | 2016-07-12 | 2018-01-18 | Tallinn University Of Technology | Self-aligned fibrous scaffolds for automechanoinduction of cell cultures |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB2605009A (en) * | 2021-03-14 | 2022-09-21 | Copner Biotech Ltd | Bio-scaffold |
| WO2022195242A1 (en) | 2021-03-14 | 2022-09-22 | Copner Biotech Ltd | Cell culture scaffold formed via 3d printing |
| GB2605009B (en) * | 2021-03-14 | 2023-04-19 | Copner Biotech Ltd | Bio-scaffold |
| GB2613976A (en) * | 2021-03-14 | 2023-06-21 | Copner Biotech Ltd | Bio-Scaffold |
| GB2613976B (en) * | 2021-03-14 | 2024-10-23 | Copner Biotech Ltd | Bio-Scaffold |
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Application publication date: 20190125 |