CN205077067U - A composite scaffold for cell culture - Google Patents
A composite scaffold for cell culture Download PDFInfo
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- CN205077067U CN205077067U CN201520551670.2U CN201520551670U CN205077067U CN 205077067 U CN205077067 U CN 205077067U CN 201520551670 U CN201520551670 U CN 201520551670U CN 205077067 U CN205077067 U CN 205077067U
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Abstract
The utility model relates to a composite scaffold for cell culture, composite scaffold include supporting structure and bearing structure, wherein, the supporting structure includes the nanofiber membranes who makes through the electrostatic spinning method, bearing structure includes that the middle part is sealed, both ends have the cavity structure of port no. 1 and port no. 2 respectively, the supporting structure laminating is in on the terminal surface of bearing structure port no. 1. The utility model discloses a composite scaffold for cell culture can simulate natural thin extracellular matrix's structural feature, and three -dimensional support is difficult for operation, poor, the yi impaired defect of rigidity in overcoming prior art.
Description
Technical field
The utility model relates to the support for cell cultures, particularly a kind of electrostatic spinning and the 3D printing technique three-dimensional rack for simulating natural extracellular matrix structure designed in conjunction.
Background technology
Cell cultures work has now been widely used in the every field such as biology, medical science, toxicology, new drug development, biotechnology, and research and the morphological structure of observation of cell and the technique means of vital movement have become screening and the preliminary experiment means of a kind of necessity of life studies.
At present, conventional cell cultures pattern comprises two-dimentional cell cultures, be carry out in Tissue Culture Plate is as 2,4,6,12,24,48,96 porocyte culture plates or Tissue Culture Flask, bio-reactor etc., cell is in the medium with a kind of two-dimensional approach monolayer adherence growth.But multinomial research finds that the genetic expression of two-dimentional cultured cells in vitro, signal transduction and morphology all may be variant with the cell of three dimensional growth in vivo.
In recent years, along with technical development, establish the cell culture processes of multiple three dimensional constitution, can fully simulate organism environment, provide the environment of appropriate cell growth.Such as nano fiber scaffold and porous support dimensional culture (porous calcium phosphate support, polylactic acid bracket, poly(lactic acid) and glycolide copolymer support, collagen scaffold, sodium alginate support etc.), three dimensional hydrogel support cultivates (such as Chinese patent application CN201110259019.4), the method such as stirring-type, tubular fibre, rotary flask cultivation.
Wherein, the three-dimensional network-like structure that the nano fiber scaffold obtained with electrostatic spinning technique has high specific surface area, high porosity because of it and is interconnected, better can simulate the constructional feature of natural extracellular matrix, for the growth of seed cell and propagation provide good microenvironment, therefore all have broad application prospects (such as Chinese patent application 200480004293.5, CN200710134505.7, CN200910050507.7, CN201110397867.1) at biological and medical field.
But, three-dimensional stent material is prepared with electrostatic spinning technique, because it is the spinning film formed under electrostatic interaction, its thickness is subject to certain restrictions, the spinning film thinner (being generally nanometer or micron order) formed, when cell cultures, is difficult to put it in fixing culture vessel in indeformable situation, also be unfavorable for that the later stage is identified the cell on spinning film, simultaneously misoperation also can cause infringement to a certain extent to spinning film.
Summary of the invention
The purpose of this utility model is to provide a kind of support for cell cultures, can simulate the constructional feature of natural extracellular matrix, and overcomes that three-dimensional rack in prior art is not easy to operate, poor rigidity, easily damaged defect.
In order to realize the purpose of this utility model, the utility model provides a kind of compound rest for cell cultures on the one hand, comprise supporting structure and supporting structure, wherein, described supporting structure comprises by the obtained nano fibrous membrane of electrospinning process, described supporting structure comprises the cavity structure that middle part is closed, two ends have port one and port two respectively, and described supporting structure is fitted on the end face of described supporting structure port one.
Preferably, described supporting structure is the nano fibrous membrane with three-dimensional porous structure.
Preferably, the diameter of described nanofiber is 100 ~ 1000nm; Be more preferably 200 ~ 600nm.
In the utility model, the material of described supporting structure be selected from following group one or more: polycaprolactone, poly(lactic acid), Poly-L-lactic acid, polyglycolic acid, polyvinyl alcohol, polycarbonate, urethane, poly phosphate, polystyrene, polyethylene terephthalate, polymethylmethacrylate, PHBV, polyhydroxybutyrate capronate, polyesteramide, polyethylene oxide, polyvinylpyrrolidone, collagen protein, chitosan, modified chitosan, starch, Mierocrystalline cellulose, modified-cellulose, gelatin, scleroproein, silk fibroin, hyaluronic acid, Lalgine, chondroitin sulfate, heparin, agar, dextran, alginic acid, be preferably polycaprolactone, poly(lactic acid), Poly-L-lactic acid, polyglycolic acid, polyvinyl alcohol, one or more in collagen protein, be more preferably polycaprolactone, polycaprolactone-collagen protein mixture.
Preferably, described supporting structure is fitted by bonding mode.Preferred, fitted by medical adhesive.
In the utility model, described laminating is also fitted by mode that is ultrasonic or melting solder; Described medical adhesive can be the polymer materials of non-cell toxicity, as urethane, natural rubber, acrylate, collagen protein etc., specifically can be, the conventional medical adhesive in this areas such as α-cyanoacrylate, polymethylmethacrylate, bone cement, aromatic series many methacrylic acids urethane, the utility model is not construed as limiting this.
Preferably, described supporting structure is by 3D printing and making.
In the utility model, supporting structure also makes by modes such as injection mouldings.Those skilled in the art know, 3D printing technique (also known as 3D rapid shaping technique or increasing material manufacturing technology) refers under the control of the computer, according to the data such as computer aided design (CAD) (CAD) model or computed tomography (CT) of object, piled up by the accurate 3D of material, manufacture the novel digital forming technique of any complicated shape 3D object fast.Wherein, fused glass pellet (FDM) adopts hot melt shower nozzle, makes the material of molten state extrude by computer-controlled path, deposit, and coagulation forming, through layer by layer deposition, solidify, finally remove propping material, obtain required three-dimensional objects.This technology has the advantages such as moulding product precision is high, surface quality good, shaping machine structure is simple, non-environmental-pollution, the supporting structure of making desired shape that can be quick, accurate, easy and structure.
Preferably, described supporting structure makes the size of applicable cel culture plates hole, culturing room, culturing bottle and/or bio-reactor.Concrete, described supporting structure can make the size in applicable cel culture plates hole, such as 2 orifice plates, 4 orifice plates, 6 orifice plates, 12 orifice plates, 24 orifice plates, 48 orifice plates and 96 orifice plates.
Preferably, the cross section of described supporting structure is circular, and vertical section is square, rectangle or trapezoidal.
Those skilled in the art know, and the cross section of described supporting structure is not limited only to circle, also can be ellipse, trilateral, square, rectangle, regular polygon etc., if its can with cultivation structure matching.Such as, when cell culture chamber is rectangular parallelepiped, supporting structure cross section is rectangle.
Preferred, the cross section of described supporting structure is circular, and vertical section is trapezoidal, and the diameter of described port one is less than the diameter of port two.
The utility model, can with reference to cel culture plates hole dimension when designing the shape and size of supporting structure.By being the diameter being less than port two by the diameter design of port one, on the one hand, be convenient to cultivate the two dimension of routine in cell experiment carry out parallel contrast experiment with the utility model dimensional culture, supporting structure is more stable in cel culture plates hole, not easily landing; On the other hand, adopt this design can make there is a segment distance between nano fibrous membrane and culture vessel, make cell better form three-dimensional structure, also can realize the Dual culture of cell.Such as, when compound rest being placed in 6 orifice plates and testing, the diameter of supporting structure port two can according to 6 plate size, the diameter of port one designs according to 12 plate size, now also mate with 12 plate size for the size of the nano fibrous membrane of cell cultures in compound rest, when carrying out parallel simultaneous test, two-dimentional comparative group adopts 12 orifice plates.In like manner, when being placed in 12 orifice plate, the diameter of port two is according to 12 plate size, and the diameter of port one designs according to 24 plate size; When being placed in 24 orifice plate, the diameter of port two is according to 24 plate size, and the diameter of port one is according to 48 plate size; The like.The explanation of concrete size is not used in restriction protection domain of the present utility model above, only for illustration of the mentality of designing of technical solutions of the utility model to illustrate some preferred embodiments.
Preferably, described supporting structure also comprises a set collar, and described Fixing shrink ring is connected on outside described port one.Preferred, described set collar is annulus, and described circle ring inner wall is provided with columnar projections, and preferably, described columnar projections is uniformly distributed along circle ring inner wall circumference.
In the utility model, arrange set collar on the support structure, this set collar can be used for nano fibrous membrane to be better fixed on end face on the one hand, makes it not come off; On the other hand, can make there is a segment distance between nano fibrous membrane and culture vessel, make cell better form three-dimensional structure, also can realize the Dual culture of cell.
Preferably, described supporting structure port two comprises outward extending edge, described edge is provided with projection, this convex role is convenient to grasp and/or mobile described supporting structure, can for ease of the handle grasped a concrete embodiment projection, can be strip, column, tabular, can be smooth or rough, the utility model does not do concrete restriction to its shape and structure.
In the utility model, the edge of supporting structure port two can be placed on cel culture plates hole, adopt handle disposed thereon to move compound rest easily.
In the utility model, the material of described supporting structure comprises non-cell toxicity and red shortness's polymer of not easily degrading as poly(lactic acid), PLLA, ABS, polymeric amide, polyester, polycarbonate, polyethylene, polypropylene, polystyrene, polycaprolactone, polycarbonate, urethane, poly phosphate, polyethylene terephthalate, polymethylmethacrylate, PHBV, polyhydroxybutyrate capronate, polyesteramide, polyethylene oxide etc.
In the utility model, described compound rest can be used alone, also can put into cell culture vessel with the use of.
In the utility model, the type of service of described compound rest is not restricted, such as, when compound rest being placed in cel culture plates hole, can independently be placed in arbitrary culture hole, also can link into an integrated entity multiple compound rest formula structure, and port two edge of each supporting structure is interconnected, form integral structure, each port one is corresponding with cell cultures hole site respectively.
In addition, the utility model also provides the method for a kind of making for the compound rest of cell cultures on the one hand, comprises the steps:
(1) prepare electrospinning liquid, under certain voltage, receiving range, flow velocity, collection time, carry out electrostatic spinning, adopt receptor to receive electrostatic spinning, obtain nano fibrous membrane supporting structure;
(2) printed by 3D or injection moulding makes supporting structure, described supporting structure comprises that middle part is closed, two ends have the cavity structure of port one and port two respectively;
(3) supporting structure that step (1) obtains is fitted on the end face of port one of the supporting structure that step (2) obtains, forms compound rest.
In the utility model, described electrospinning liquid is prepared by the following method: be dissolved in certain solvent by timbering material, obtains electrospinning liquid, wherein, described timbering material be selected from following group one or more: polycaprolactone, poly(lactic acid), Poly-L-lactic acid, polyglycolic acid, polyvinyl alcohol, polycarbonate, urethane, poly phosphate, polystyrene, polyethylene terephthalate, polymethylmethacrylate, PHBV, polyhydroxybutyrate capronate, polyesteramide, polyethylene oxide, polyvinylpyrrolidone, collagen protein, chitosan, modified chitosan, starch, Mierocrystalline cellulose, modified-cellulose, gelatin, scleroproein, silk fibroin, hyaluronic acid, Lalgine, chondroitin sulfate, heparin, agar, dextran, alginic acid, be preferably polycaprolactone, poly(lactic acid), Poly-L-lactic acid, polyglycolic acid, polyvinyl alcohol, one or more in collagen protein, be more preferably polycaprolactone, polycaprolactone-collagen protein mixture.
Described solvent can be the mixture of formic acid, acetic acid, ethanol, acetone, dimethyl formamide, N,N-DIMETHYLACETAMIDE, dimethyl sulfoxide (DMSO), hexafluoroisopropanol, trifluoroethanol, methylene dichloride, trichloromethane, methyl alcohol, ethanol, chloroform, diox, Halothane, trifluoroacetic acid, water or their arbitrary proportions.Above-mentioned timbering material and the weight of solvent and ratio etc. can refer to prior art.
Preferably, electrospinning liquid described in step (1) is the hexafluoroisopropanol solution of polycaprolactone or polycaprolactone-collagen protein mixture.Described polycaprolactone and collagen protein are 1 ~ 3:1 by weight, and be preferably 1.5 ~ 2.5:1, the concentration of solution is 5 ~ 10%, are preferably 6 ~ 8%.
Preferably, described in step (1), the parameter of electrostatic spinning is: voltage 5 ~ 40KV, receiving range 5 ~ 25cm, flow velocity 0.2 ~ 2.0mL/h, collection time 0.2 ~ 2h.Preferred, voltage 10 ~ 20KV, receiving range 10 ~ 20cm, flow velocity 0.5 ~ 1mL/h, collection time 0.5 ~ 1h.
Preferably, in step (1), receptor is PBS damping fluid or cover glass.
Preferably, step (2) is by 3D printing and making supporting structure.Preferably, go out 3D supporting structure by Computer Design, adopt hot melt shower nozzle, make the material of molten state extrude by computer-controlled path, deposit, and coagulation forming, be supported structure.Concrete 3D Method of printing can refer to state of the art, and the utility model does not repeat this.
Preferably, the utility model support structure designs is the size of applicable cel culture plates hole, culturing room, culturing bottle and/or bio-reactor, specifically can be the foregoing supporting structure of the utility model.
The material of supporting structure described in the utility model comprises non-cell toxicity and red shortness's polymer of not easily degrading as poly(lactic acid), PLLA, ABS, polymeric amide, polyester, polycarbonate, polyethylene, polypropylene, polystyrene, polycaprolactone, polycarbonate, urethane, poly phosphate, polyethylene terephthalate, polymethylmethacrylate, PHBV, polyhydroxybutyrate capronate, polyesteramide, polyethylene oxide etc.Be preferably poly(lactic acid).
Preferably, fitted by bonding mode in step (3).Preferred, fitted by medical adhesive.
In the utility model, described laminating is also fitted by mode that is ultrasonic or melting solder; Described medical adhesive can be the polymer materials of non-cell toxicity, as urethane, natural rubber, acrylate, collagen protein etc., specifically can be, the conventional medical adhesive in this areas such as α-cyanoacrylate, polymethylmethacrylate, bone cement, aromatic series many methacrylic acids urethane, the utility model is not construed as limiting this.
The utility model beneficial effect:
1. the utility model compound rest comprises the three-dimensional nanofiber membrane supporting structure obtaining having vesicular structure by electrostatic spinning, can be good at the constructional feature simulating natural extracellular matrix.
2. design supporting structure in the utility model compound rest, by supporting structure laminating on the support structure, overcome that three-dimensional rack in prior art is not easy to operate, poor rigidity, easily damaged defect; Make supporting structure can bear normal power operation in transfer and cell cultures, ensure that the structure of the porous of support does not deform with impaired.
3. the utility model compound rest can independently use or with the cell culture apparatus of routine with the use of, and pass through structure and the size of appropriate design supporting structure, better can cultivate with two dimension and compare, in addition, the object of two dimension, three-dimensional Dual culture can also be realized.
Accompanying drawing explanation
The structural representation of Fig. 1 the utility model compound rest
Fig. 2 the utility model supporting structure schematic diagram
Fig. 3 the utility model supporting structure three-view diagram
Fig. 4 the utility model set collar structural representation
Embodiment
Below in conjunction with specific embodiment and accompanying drawing, the utility model is further elaborated; following examples are only not used in restriction scope of the present utility model for illustration of the utility model, those of ordinary skill in the art equivalent transformation or equivalent substitution done by embodiment are all included in protection domain of the present utility model.
See accompanying drawing 1-2, the compound rest 1 that the utility model is used for cell cultures comprises supporting structure 2 and supporting structure 3, wherein, supporting structure 2 comprises by the obtained nano fibrous membrane of electrospinning process, supporting structure 3 comprises the cavity structure 6 that middle part is closed, two ends have port 1 and port 25 respectively, and supporting structure 2 is fitted on the end face of supporting structure 3 port 1.
In the utility model, supporting structure makes the size of applicable cel culture plates hole, culturing room, culturing bottle and/or bio-reactor.In a concrete embodiment, supporting structure can make the size in applicable cel culture plates hole, such as 6 orifice plates, 12 orifice plates, 24 orifice plates, 48 orifice plates and 96 orifice plates.
In a concrete embodiment, support structure designs is tubular, its cross section for circular, vertical section be rectangle.
In a concrete embodiment, see accompanying drawing 3, the cross section of supporting structure is circular, and vertical section is trapezoidal, and the diameter of port 1 is less than the diameter of port 25.
Adopt this design, by being the diameter being less than port two by the diameter design of port one, on the one hand, be convenient to cultivate the two dimension of routine in cell experiment carry out parallel contrast experiment with the utility model dimensional culture, supporting structure is more stable in cel culture plates hole, not easily landing; On the other hand, adopt this design can make there is a segment distance between nano fibrous membrane and culture vessel, make cell better form three-dimensional structure, also can realize the Dual culture of cell.
The design of the size and dimension of the utility model supporting structure is described for cel culture plates hole, when compound rest being placed in 6 orifice plates and testing, the diameter of supporting structure port two can according to 6 plate size, the diameter of port one designs according to 12 plate size, now also mate with 12 plate size for the size of the nano fibrous membrane of cell cultures in compound rest, when carrying out parallel simultaneous test, two-dimentional comparative group adopts 12 orifice plates.In like manner, when being placed in 12 orifice plate, the diameter of port two is according to 12 plate size, and the diameter of port one designs according to 24 plate size; When being placed in 24 orifice plate, the diameter of port two is according to 24 plate size, and the diameter of port one is according to 48 plate size; The like.
In a concrete embodiment, see accompanying drawing 4, supporting structure also comprises a set collar 7, and set collar 7 is socketed in outside port 1.Concrete, set collar 7 can be annulus, and circle ring inner wall is provided with columnar projections 8, and columnar projections 8 is uniformly distributed along circle ring inner wall circumference.
The utility model arranges set collar on the support structure, and this set collar can be used for nano fibrous membrane to be better fixed on end face on the one hand, makes it not come off; On the other hand, can make there is a segment distance between nano fibrous membrane and culture vessel, make cell better form three-dimensional structure, also can realize the Dual culture of cell.
In a concrete embodiment, see accompanying drawing 1-3, supporting structure port 25 comprises outward extending edge 9, edge 9 is provided with the handle 10 being convenient to grasp.In use, the edge of supporting structure port two can be placed on cel culture plates hole, adopt handle disposed thereon to move compound rest easily.
The making of embodiment 1:PCL nano fibrous membrane supporting structure
Electrostatic spinning technique is utilized to carry out electrostatic spinning.Electrostatic spinning test conditions is as follows: with hexafluoroisopropanol (HFIP) for solvent, prepare 6% polycaprolactone (PCL) solution, receiving range is 20.5cm, flow velocity is 1.0mL/h, and voltage is 9.5kv-10.0kv, and collection time is 0.5h, nitrogen pressure is 0.1Mpa, obtain nano fibrous membrane with PBS damping fluid for receptor, obtain supporting structure after drying, to obtain nano fibrous membrane be diameter is 300nm+/-20nm.
The making of embodiment 2:PCL-collagen protein nano fibrous membrane supporting structure
Electrostatic spinning technique is utilized to carry out electrostatic spinning.Electrostatic spinning test conditions is as follows: take HFIP as solvent, preparation 8%PCL-collagen solution, and PCL and collagen protein ratio are 11:5, receiving range is 20.5cm, flow velocity is 0.5mL/h, and voltage is 9.5kv-10.0kv, and collection time is 0.5h, nitrogen pressure is 0.1Mpa, with PBS damping fluid for receptor, to obtain spinning film be diameter is 560nm+/-20nm, and diameter is thicker, simultaneously because collagen has certain wetting ability, be beneficial to the growth of cell.
Embodiment 3: the making of tubular construction PLA supporting structure
Print with poly(lactic acid) (PLA) for raw material by 3D, according to size and the structure design supporting structure of cel culture plates hole 6,24,96 orifice plate.Size is as shown in table 1 below:
Wherein column construction needs static spinning membrane medical science viscose to adhere to cylindrical bottom not with handles equally, and wherein it is as shown in the table for column size:
Table 1: the tubular supporting structure size of mating with 6,24,96 orifice plates
| Parameter (unit mm) | 6 orifice plates | 24 orifice plates | 96 orifice plates |
| Port one diameter | 34.0 | 14.0 | 5.6 |
| Thickness | 1.0 | 1.0 | 0.5 |
| Port two edge length | 2.0 | 2.0 | 1.0 |
| Port two edge thickness | 1.0 | 1.0 | 0.5 |
| Handle length | 4.0 | 3.0 | 3.0 |
| Highly | 13.05 | 13.05 | 13.05 |
Embodiment 4: the making of trapezoid cross section PLA supporting structure
Print with poly(lactic acid) (PLA) for raw material by 3D, according to size and the structure design supporting structure of cel culture plates hole 6,12,24 orifice plate.Size is as shown in table 2 below:
Table 2: the supporting structure size of mating with 6,12,24 orifice plates
| Parameter (unit mm) | 6 orifice plates | 12 orifice plates | 24 orifice plates |
| Port two diameter | 31.07 | 19.09 | 13.55 |
| Port one diameter | 23.79 | 16.61 | 11.21 |
| Thickness | 0.80 | 0.50 | 0.50 |
| Port two edge length | 3.34 | 2.00 | 2.00 |
| Port two edge thickness | 1.00 | 1.00 | 1.00 |
| Handle length | 2.66 | 2.04 | 3.00 |
| Highly | 13.05 | 13.05 | 13.05 |
Embodiment 5: containing the making of the PLA supporting structure of set collar
Print with poly(lactic acid) (PLA) for raw material by 3D, according to size and the structure design supporting structure of cel culture plates hole 6,24 orifice plate, in the present embodiment, set collar is annulus, is designated as outer shroud, and the main body of supporting structure is inner ring.Size is as shown in table 3 below:
Table 3: the supporting structure size of mating with 6,24 orifice plates
Embodiment 6: neuroma parent cell and glioma cells culture experiment
Adopt supporting structure described in the utility model embodiment 1 as the support of three-dimensional cell cultivation, test for the supporting structure of 6 orifice plate couplings of the utility model design, medical adhesive laminating is adopted by PCL nano fibrous membrane supporting structure to prepare compound rest on the support structure, cell culture test is carried out to neuroma parent cell core neurogliocyte, and CCK8 detection and life or death staining examine are carried out respectively to it.
As shown in table 4, be the CCK-8 detected result of neuroma parent cell SY5Y, control group is two dimension cultivation and another product of our company (adopted by static spinning membrane sizing agent to paste on rigid plastics, be designated as PCL-plastics); As shown in table 5, be the CCK-8 detected result of neurogliocyte, control group is that two dimension is cultivated and the centrifugal cultivation of three-dimensional rack.
Table 4: the CCK-8 detected result of neuroma parent cell SY5Y
Table 5: the CCK-8 detected result of neurogliocyte
From the above results, for neuroma parent cell, wherein adopt the cell growth state under two kinds of processing modes of PCL three-dimensional rack good, compared with cultivating with 2D, the 3rd day to the 6th day cell growth velocity on PCL is obviously higher; Carried out the life or death dyeing of two kinds of cells, coloration result also shows that culturing cell is not toxic to cell within this material simultaneously; In addition, adopt the utility model PCL compound rest product compared with directly being pasted on rigid plastics by PCL, adopt the utility model structure PCL outward appearance more evenly and structure is not easily destroyed.
For neurogliocyte, under comparing static and centrifugal vaccination ways, cell growth state.Spongiocyte belongs to primary cell strain, so competence for added value is not strong, PCL material is to cytotoxic, but competence for added value is limited.From the results of view under centrifugal method, cell had a reasonable proliferation rate at the 3rd day by the 5th day, but on the whole differed greatly between each hole under centrifugal vaccination ways, and collimation is bad.Therefore, dibbling can be adopted in test to leave standstill method inoculating cell.This material well can be selected as the one of primary cell dimensional culture.
Claims (9)
1. the compound rest for cell cultures, comprise supporting structure and supporting structure, wherein, described supporting structure comprises by the obtained nano fibrous membrane of electrospinning process, described supporting structure comprises the cavity structure that middle part is closed, two ends have port one and port two respectively, and described supporting structure is fitted on the end face of described supporting structure port one.
2. compound rest as claimed in claim 1, is characterized in that: described supporting structure is the nano fibrous membrane with three-dimensional porous structure.
3. compound rest as claimed in claim 2, is characterized in that: the diameter of described nanofiber is 100 ~ 1000nm.
4. the compound rest as described in any one of claim 1-3, it is characterized in that: the material of described supporting structure is selected from the one in following group: polycaprolactone, poly(lactic acid), Poly-L-lactic acid, polyglycolic acid, polyvinyl alcohol, polycarbonate, urethane, poly phosphate, polystyrene, polyethylene terephthalate, polymethylmethacrylate, PHBV, polyhydroxybutyrate capronate, polyesteramide, polyethylene oxide, polyvinylpyrrolidone, collagen protein, chitosan, modified chitosan, starch, Mierocrystalline cellulose, modified-cellulose, gelatin, scleroproein, silk fibroin, hyaluronic acid, Lalgine, chondroitin sulfate, heparin, agar, dextran, alginic acid.
5. compound rest as claimed in claim 1, is characterized in that: described supporting structure is by 3D printing and making.
6. compound rest as claimed in claim 5, is characterized in that: the cross section of described supporting structure is for circular, and vertical section is square, rectangle or trapezoidal.
7. compound rest as claimed in claim 6, is characterized in that: the cross section of described supporting structure is for circular, and vertical section is trapezoidal, and the diameter of described port one is less than the diameter of port two.
8. the compound rest as described in any one of claim 5-7, is characterized in that: described supporting structure port two comprises outward extending edge, and described edge is provided with projection.
9. the compound rest as described in any one of claim 5-7, is characterized in that: described supporting structure also comprises a set collar, and described Fixing shrink ring is connected on outside described port one, and described set collar inwall is provided with columnar projections.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105238735A (en) * | 2015-11-13 | 2016-01-13 | 广州洁特生物过滤股份有限公司 | Three-dimensional cell culture stent and preparation method thereof |
| CN105882036A (en) * | 2016-04-08 | 2016-08-24 | 天津大学 | Programmable three-dimensional superfine fiber network material and preparation method |
| CN106512065A (en) * | 2016-10-20 | 2017-03-22 | 天津卫凯生物工程有限公司 | Three-dimensional scaffold applied to cell culture and preparation method thereof |
| CN109395095A (en) * | 2017-09-18 | 2019-03-01 | 武汉原生原代生物医药科技有限公司 | In vivo with biomembrane and its preparation method and application |
-
2015
- 2015-07-27 CN CN201520551670.2U patent/CN205077067U/en not_active Expired - Fee Related
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105238735A (en) * | 2015-11-13 | 2016-01-13 | 广州洁特生物过滤股份有限公司 | Three-dimensional cell culture stent and preparation method thereof |
| US11168294B2 (en) | 2015-11-13 | 2021-11-09 | Guangzhou Jet Bio-Filtration Co., Ltd. | Three-dimensional cell culture scaffold and preparation method thereof |
| CN105882036A (en) * | 2016-04-08 | 2016-08-24 | 天津大学 | Programmable three-dimensional superfine fiber network material and preparation method |
| CN106512065A (en) * | 2016-10-20 | 2017-03-22 | 天津卫凯生物工程有限公司 | Three-dimensional scaffold applied to cell culture and preparation method thereof |
| CN109395095A (en) * | 2017-09-18 | 2019-03-01 | 武汉原生原代生物医药科技有限公司 | In vivo with biomembrane and its preparation method and application |
| CN109395095B (en) * | 2017-09-18 | 2021-09-24 | 武汉原生原代生物医药科技有限公司 | In vivo biological membrane and preparation method and application thereof |
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