CN109258984A - Application of the cinnamic acid with immune growth promoting function in Cynoglossus semilaevis feed - Google Patents
Application of the cinnamic acid with immune growth promoting function in Cynoglossus semilaevis feed Download PDFInfo
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- CN109258984A CN109258984A CN201811167672.6A CN201811167672A CN109258984A CN 109258984 A CN109258984 A CN 109258984A CN 201811167672 A CN201811167672 A CN 201811167672A CN 109258984 A CN109258984 A CN 109258984A
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- cynoglossus semilaevis
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/11—Aldehydes
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P37/00—Drugs for immunological or allergic disorders
- A61P37/02—Immunomodulators
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
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Abstract
The invention discloses have application of the cinnamic acid of immune growth promoting function in Cynoglossus semilaevis feed.In per kilogram feed the additive amount of cinnamic acid be 100~1000mg, continuous feeding Cynoglossus semilaevis 10 days or more.Experiments have shown that the continuous feeding Cynoglossus semilaevis of cinnamic acid 10 days or more, Cynoglossus semilaevis immunity function can be significantly improved, pathogen invasion is effective against.Disease incidence is reduced to 2% hereinafter, cultivation survival rate is increased to 98.2% or more；Food intaking of Banhuashe Soleidae fish rate and efficiency of feed utilization are significantly improved, the time of ingesting shortens 50% or more, and grazing rate improves 36.67% or more, and feed coefficient reduces by 14.39% or more, and specific growth rate improves 21.74% or more；Antibiotic and chemosterilant dosage can be effectively reduced, reduce environmental pollution, there are good ecological benefits.
Description
Technical field
The invention belongs to aquatic feeds technical fields more particularly to a kind of cinnamic acid with immune growth promoting function half
Application in sliding-tongue sole feed.
Background technique
Cynoglossus semilaevis belongs to Pleuronectiformes, Cynoglossidae, and tongue sole category is commonly called as field sowthistle herb, is distributed in China Coast sea area, and half
Sliding-tongue sole individual is big, growth is fast, meat flavour is delicious, and economic value is very high, for the rare seawater fish of China, has anti-to water environment
Inverse ability is strong, nutritional requirement is low, the speed of growth is fast, and fish body meat content is big to wait many merits, therefore its intensive culture in recent years
Scale is increasing.With being gradually increased for cultured output, disease problem is got worse.The chemistry such as various antibiotic, disinfectant
Drug abuse causes the generation and outburst of drug resistance cause of disease in microbiologic population, reduces the curative effect of antibiotic, cause in aquatic products
Left drug endangers human health, and leads to water environment pollution, increases publilc health risk.Therefore, for sustainable
For culture fishery, it is extremely urgent to seek disease control new method.
Cinnamic acid also known as cinnaldehydrum are the main actives (content > 75%) of traditional Chinese medicine Cortex Cinnamomi volatile oil.Cortex cinnamomi is
The dry bark of Lauraceae aiphyllium plant cortex cinnamomi, wherein the cinnamon oil output in Guangdong and Guangxi Provinces accounts for about 80% of world wide production or so.
Cinnamic acid molecular weight is 132.15, molecular formula C9H8O is colourless or yellowish or chartreuse oily liquids, has strongly
Cortex cinnamomi fragrance, it is sweet and peppery and have burning sensation, be insoluble in water and glycerol, be dissolved in propylene glycol, be miscible in ethyl alcohol, ether, chloroform,
Expressed oi and volatile oil can volatilize with vapor.Cinnamic acid has restraining and sterilizing bacteria, and anti-inflammatory is antitumor, enhances immunity of organisms
Etc. multiple biological functions, be usually used in improving efficiency of feed utilization in production, promote livestock birds health and growth, promote intestinal beneficial
Flora growth prevents feed quality from reducing.Cinnamic acid has is metabolized fast, noresidue in animal body, pollution-free to breeding environment,
A kind of the advantages that pathogen is not likely to produce drug resistance to it, it is considered to be novel green, environmentally friendly feed addictive.
Summary of the invention
The purpose of the present invention is to provide a kind of cinnamic acids with immune growth promoting function in Cynoglossus semilaevis feed
Using lacking a kind of skill of Immune enhancement means for Cynoglossus semilaevis during the cynoglossus semilaevis cultivation for solving the prior art
Art problem；If solving conventional Cynoglossus semilaevis anti-pathogenic drug long-time service, there may be drug resistances；It is sliding to solve conventional half
Tongue sole anti-pathogenic drug may be enriched in Cynoglossus semilaevis fish body, and then influence the edible health of the mankind.
In order to achieve the above objectives, the present invention adopts the following technical scheme that: having the cinnamic acid of immune growth promoting function
Application in Cynoglossus semilaevis feed.The additive amount of cinnamic acid is 100~1000mg, continuous half sliding-tongue of feeding in per kilogram feed
Sole 10 days or more.
The beneficial effects of the present invention are: having determined using cinnamic acid as Cynoglossus semilaevis Immune enhancement drug and growth promotion drug
Application, can effectively promote its disease-resistant performance on the basis of improving Cynoglossus semilaevis non-specific immunity, promote Cynoglossus semilaevis
Growth.Beneficial technical effect is obtained the present invention is based on rigorous laboratory facilities, while cost is relatively low, is easily achieved, therefore
With good promotion prospect.
Specific embodiment
It elaborates combined with specific embodiments below to the present invention.
The present invention adopts the following technical scheme that: having the cinnamic acid of immune growth promoting function in Cynoglossus semilaevis feed
Using, in per kilogram feed the additive amount of cinnamic acid be 100~1000mg, continuous feeding Cynoglossus semilaevis 10 days or more.
In the examples below, used cinnamic acid comes from commercially available, and rank is food-grade.
1, the acquisition of cinnamic acid preparation
2g sucrose ester is taken, distilled water 4mL stirring swelling is added, the 5% of 70~80 DEG C is then added under the conditions of magnetic agitation
Ethyl alcohol about 240mL, stirring are cooled to 40 DEG C or so, are slowly added to 36g beta-cyclodextrin, and preservative film sealed after beta-cyclodextrin adds
Continue to stir 10min, cinnamic acid and V are then added during agitationEMixture (12mL cinnamic acid+1mL VE), then
It is put into ultrasonic wave and handles 15min, gone on magnetic stirring apparatus later and continue to stir 1h.Gained cinnamic acid embeds in product, meat
Cinnamic aldehyde content is 40mg/mL.
2, cinnamic acid preparation improves Cynoglossus semilaevis immunity function
The preparation of cinnamic acid preparation, according to percent by volume, cinnamic acid 4%, sucrose ester 0.67%, VE0.33%, β-ring
Dextrin 12%, 5% concentration ethanol 83%.Added respectively in the every kilogram of feed of cinnamic acid preparation 0.263ml, 2.63ml,
26.3ml, 78.947ml (additive amount for being equivalent to cinnamic acid in per kilogram feed is 10,100,1000,3000mg).It is continuous to feed
Eating initial weight is to find after Cynoglossus semilaevis 30 days of 100g ± 5g, compared with the control group for not adding the feed addictive,
Additive amount is that 10mg/kg Cynoglossus semilaevis NBT positive cell quantity reduces by 47.62%, and blood plasma lysozyme activity reduces by 28.77%,
Total number born activity reduces by 99.28%, and catalase activity reduces by 76.72%, MDA content and improves 12.27%,
Statistical experiment group Cynoglossus semilaevis survival rate is 88% or more, 10% or less disease incidence.Additive amount is 100mg/kg Cynoglossus semilaevis NBT
Positive cell quantity improves 23.81%, and blood plasma lysozyme activity improves 97.14%, and total number born activity improves
72.78%, catalase activity, which improves 44.74%, MDA content, reduces by 45.26%, statistical experiment group Cynoglossus semilaevis survival rate
It improves to 97.6% or more, disease incidence is reduced to 2% or less.Additive amount is 1000mg/kg Cynoglossus semilaevis NBT positive cell quantity
33% is improved, blood plasma lysozyme activity improves 113.3%, and total number born activity improves 83.8%, catalase
Activity, which improves 65.6%, MDA content, reduces by 50.2%, and statistical experiment group Cynoglossus semilaevis survival rate is improved to 98.2% or more, sends out
Sick rate is reduced to 1.8% or less.Additive amount is that 3000mg/kg Cynoglossus semilaevis NBT positive cell quantity reduces by 61.90%, blood plasma
Lysozyme activity reduces by 44.40%, and total number born activity reduces by 40.30%, and catalase activity reduces
77.33%, MDA content improve 51.30%, and statistical experiment group Cynoglossus semilaevis survival rate is 86% or more, 10% or less disease incidence.
The result and immune indexes that wherein 100-1000mg/kg group Cynoglossus semilaevis death rate of the onset reduces, survival rate improves significantly improve
Experimental result it is consistent, illustrate the feeding cinnamic acid preparation in this concentration range, Cynoglossus semilaevis immunity of organism can be effectively improved
Function improves resistance.Below or above the non-specific immunity that this range of concentrations significantly inhibits Cynoglossus semilaevis.
3, cinnamic acid preparation promotes Cynoglossus semilaevis growth
The continuous initial weight of feeding finds after being Cynoglossus semilaevis 60 days of 100g ± 5g, and does not add the feed addictive
Control group compare, additive amount be 10mg/kg food intaking of Banhuashe Soleidae fish rate reduce 56.67%, specific growth rate reduces
33.33%.Feed coefficient improves 16.42%, and the time of ingesting is constant, and additive amount is the raising of 100mg/kg food intaking of Banhuashe Soleidae fish rate
36.67%, specific growth rate improves 21.74%.Feed coefficient reduces 16.42%, and the time of ingesting shortens 50%, adds
Dosage is that 1000mg/kg food intaking of Banhuashe Soleidae fish rate improves 50%, and specific growth rate improves 28.26%.Feed coefficient reduces
14.0%, the time of ingesting shortens 50%, and additive amount is that 3000mg/kg food intaking of Banhuashe Soleidae fish rate reduces 65%, particular growth
Rate reduces 57.41%.Feed coefficient improves 25.37%, and the time of ingesting improves 33.33%.Illustrate that feeding concentration is
The cinnamic acid preparation of 100-1000mg/kg can remarkably promote Cynoglossus semilaevis growth.Significantly not below or above this range of concentrations
Conducive to the growth of Cynoglossus semilaevis.
Illustrated by above embodiments, additive capacity of the cinnamic acid in aquatic feeds is in (100-1000mg/kg) range
The interior growth that can significantly improve Cynoglossus semilaevis and immunity function.Too low (10mg/kg) or excessively high (3000mg/kg) can be significant
Growth and the immunity function of Cynoglossus semilaevis are reduced, its is more acurrate compared with 0-10g/kg range, more there is reference value.
Experiments have shown that with (the continuous feeding Cynoglossus semilaevis of feed 10 days or more of addition cinnamic acid, can significantly improve half sliding-tongue
Sole immunity function is effective against pathogen invasion, and wherein NBT positive cell quantity improves 23.81% or more, and antalzyme activity improves
97.14% or more, total number born vigor improve 72.78% or more, activity of catalase improve 44.74% with
On, mda content reduces by 45.26% or more.Disease incidence is reduced to 2% hereinafter, cultivation survival rate is increased to 98.2% or more；
Significantly improve food intaking of Banhuashe Soleidae fish rate and efficiency of feed utilization, ingest the time shorten 50% or more, grazing rate improve 36.67% with
On, feed coefficient reduces by 14.39% or more, and specific growth rate improves 21.74% or more；(antibiotic can be effectively reduced in cinnamic acid
It with chemosterilant dosage, reduces environmental pollution, there are good ecological benefits
Above-described embodiment is merely to illustrate technical idea and feature of the invention, and its object is to make this field
Interior technical staff only cannot limit patent of the invention with the present embodiment it will be appreciated that the contents of the present invention and implement accordingly
Range, i.e., same changes or modifications made by all disclosed spirit, still falls in the scope of the patents of the invention.
Claims (2)
1. having application of the cinnamic acid of immune growth promoting function in Cynoglossus semilaevis feed.
2. there is application of the cinnamic acid of immune growth promoting function in Cynoglossus semilaevis feed according to claim 1, it is special
Sign is, the additive amount of cinnamic acid is 100~1000mg in per kilogram feed, continuous feeding Cynoglossus semilaevis 10 days or more.
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| CN201811167672.6A CN109258984A (en) | 2018-10-08 | 2018-10-08 | Application of the cinnamic acid with immune growth promoting function in Cynoglossus semilaevis feed |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113080111A (en) * | 2021-04-15 | 2021-07-09 | 山东龙昌动物保健食品有限公司 | Application of bile acid in improving intestinal health and cultivation survival rate of cynoglossus semilaevis |
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| CN102469808A (en) * | 2009-07-17 | 2012-05-23 | 帝斯曼知识产权资产管理有限公司 | Use of natural substances as feed additives for aquatic animals |
| TW201517795A (en) * | 2013-11-04 | 2015-05-16 | Nanosome Enterprise Ltd Com | Nanoparticle encapsulation of cinnamaldehyde, manufacturing method and application thereof |
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| CN113080111A (en) * | 2021-04-15 | 2021-07-09 | 山东龙昌动物保健食品有限公司 | Application of bile acid in improving intestinal health and cultivation survival rate of cynoglossus semilaevis |
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