CN109239245A - A kind of chromatography column holder - Google Patents
A kind of chromatography column holder Download PDFInfo
- Publication number
- CN109239245A CN109239245A CN201710556923.9A CN201710556923A CN109239245A CN 109239245 A CN109239245 A CN 109239245A CN 201710556923 A CN201710556923 A CN 201710556923A CN 109239245 A CN109239245 A CN 109239245A
- Authority
- CN
- China
- Prior art keywords
- chromatographic column
- ontology
- way connection
- chromatography column
- accommodation groove
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000004587 chromatography analysis Methods 0.000 title claims abstract description 31
- 230000004308 accommodation Effects 0.000 claims abstract description 23
- 239000000523 sample Substances 0.000 claims description 13
- 238000010438 heat treatment Methods 0.000 claims description 11
- 239000012071 phase Substances 0.000 description 13
- 239000007788 liquid Substances 0.000 description 9
- 238000010586 diagram Methods 0.000 description 7
- 230000008676 import Effects 0.000 description 7
- 238000003780 insertion Methods 0.000 description 5
- 230000037431 insertion Effects 0.000 description 5
- 230000000694 effects Effects 0.000 description 4
- 238000000926 separation method Methods 0.000 description 3
- 238000005452 bending Methods 0.000 description 2
- 150000001875 compounds Chemical class 0.000 description 2
- 239000000284 extract Substances 0.000 description 2
- 238000004128 high performance liquid chromatography Methods 0.000 description 2
- 238000001819 mass spectrum Methods 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 239000010453 quartz Substances 0.000 description 2
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N silicon dioxide Inorganic materials O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 1
- 238000004895 liquid chromatography mass spectrometry Methods 0.000 description 1
- 239000007791 liquid phase Substances 0.000 description 1
- 238000001228 spectrum Methods 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/60—Construction of the column
- G01N30/6047—Construction of the column with supporting means; Holders
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/26—Conditioning of the fluid carrier; Flow patterns
- G01N30/28—Control of physical parameters of the fluid carrier
- G01N30/30—Control of physical parameters of the fluid carrier of temperature
Abstract
The invention discloses a kind of chromatography column holders, including ontology, the ontology is equipped with the cross recess for accommodating the accommodation groove of the chromatographic column and for placing three-way connection or four-way connection, the accommodation groove is connected with cross recess, and the accommodation groove has the opening that the tail portion for the chromatographic column is stretched out.Using chromatography column holder of the invention, can orderly be stored for the chromatographic column of different length.
Description
Technical field
The present invention relates to LC-MS fields, in particular to a kind of chromatography column holder.
Background technique
Currently, the Main Analysis instrument of proteomics research is that nanoliter level high performance liquid chromatography and high resolution mass spectrum are combined
System, the system include liquid chromatograph, ion source and mass spectrometer detector, and liquid chromatograph can be by sample to be tested each component
Separation, ion source can ionize the sample to be tested each component after separating by liquid chromatograph, and mass spectrometer detector can
Sample to be tested each component after ionization is analyzed one by one, obtains the information such as molecular weight, structure and the concentration of sample to be tested.It should
In system, the mobile phase containing sample to be tested comes out from liquid chromatograph and is delivered to ion source, and electric under the action of the DC voltage
Mass spectrometer detector is delivered to through chromatographic column from after to be analyzed.Wherein, chromatographic column uses quartz capillary voluntarily hand-made more,
For its length from 10cm-300cm etc., chromatographic column can be placed in the support that in ion source and need to obtain bracket.
Fig. 1 is the structural schematic diagram of the chromatography column holder of the prior art；Fig. 2 is the front-view schematic diagram of Fig. 1.Referring to Fig. 1, figure
2, the chromatography column holder of the prior art includes the round bar 1 of a root long 15cm, which is fixed in ion source, and one end is equipped with
Three-way connection 2, the other end are fixed on the fixed part 3 of ion source, two imports 201,202 of three-way connection 2 be separately connected from
The DC voltage connector (not shown) for the mobile phase pipeline (not shown) and ion source that liquid chromatograph extracts,
Outlet 203 then connects chromatographic column 4.The head 401 of chromatographic column 4 is connected with the outlet 203 of the three-way connection 2, the tail portion of chromatographic column 4
402 extend to the entrance of mass spectrometer detector, and as shown in Figure 1 and Figure 2, chromatographic column 4 can ride on the round bar 1 to be supported.Cause
This, existing chromatography column holder is simply possible to use in the chromatography that bearing length is no more than 15cm due to the limitation of 1 length of round bar
Column.If being more than the chromatographic column of 15cm by length, such as homemade long chromatographic column rides on the round bar 1, chromatographic column pendulum will cause
Put it is unordered, not only increase operator dismount chromatographic column workload, be also easy to lead to the maloperation of operator and damage
Chromatographic column.
In addition, nanoliter level high performance liquid chromatography and high resolution mass spectrum combined system are that chromatographic column is directly exposed to external sky
In gas, it on the one hand will cause chromatographic column barrel temperatures and fluctuated with the change of environment temperature, seriously affect the steady of chromatography post separation
Qualitative, reproducibility；On the other hand chromatographic column barrel temperatures can be made to be unevenly distributed, the temperature of different parts has dramatically different, color
The spectrum longer difference of column is more obvious, and the difference of this temperature can seriously reduce the splitter effect of chromatographic column.And existing chromatographic column branch
Holder function is single, only has the function of supporting chromatographic column, cannot achieve to chromatographic column heating function, and user is to realize heating function
Entire ion source can must be bought and replace, at high cost and applicability is not strong.
For the above problem in the presence of the prior art, the new chromatography column holder of one kind is provided and is of great significance.
Summary of the invention
To solve the above problems, the present invention provides a kind of chromatography column holder, can for different length chromatographic column it is orderly
Storage.
To achieve the above object, chromatography column holder of the invention, including ontology, the ontology are equipped with described for accommodating
The accommodation groove of chromatographic column and cross recess for placing three-way connection or four-way connection, the accommodation groove are connected with cross recess
Logical, the accommodation groove has the opening that the tail portion for the chromatographic column is stretched out.
Preferably, the accommodation groove is oval.
Preferably, the lower part of the ontology offers the first blind hole for heating rod insertion.
Further, the lower part of the ontology offers the second blind hole for temp probe insertion.
Preferably, the ontology is fixed in ion source.
Chromatography column holder of the invention which is provided with the accommodation groove for accommodating chromatographic column, the chromatographic column disk of different length
It can be placed in the accommodation groove after, avoid chromatographic column and put unordered and be easy to be risk of damage to.In addition, the chromatographic column branch
The lower part of frame may be implemented equipped with the first blind hole being inserted into for heating rod and for the second blind hole of temp probe insertion to chromatographic column
Heating and temperature controlling function, column effect, stability and the reproducibility of chromatography post separation can not only be improved, additionally it is possible to avoid replacement from
Component, save the cost.
Detailed description of the invention
Fig. 1 is the structural schematic diagram of the chromatography column holder of the prior art；
Fig. 2 is the front-view schematic diagram of Fig. 1；
Fig. 3 is the structural schematic diagram of chromatography column holder of the invention, and it illustrates chromatographic columns and three-way connection to be put in ontology
On state；
Fig. 4 is the front-view schematic diagram of chromatography column holder of the invention；
Fig. 5 is the schematic side view of chromatography column holder of the invention；
Fig. 6 is a kind of mounting structure schematic diagram of embodiment of chromatography column holder of the invention.
Specific embodiment
In the following, being further described in conjunction with attached drawing to structure and working principle etc. of the invention.
Referring to Fig. 3~Fig. 5, chromatography column holder of the invention includes ontology 5, which is equipped with for accommodating chromatographic column 4
Accommodation groove 501, accommodation groove 501 have for chromatographic column 4 tail portion 402 stretch out opening 5011.As shown in figure 3, can be by chromatography
Column 4 is placed in accommodation groove 501 after coiling, the head 401 of chromatographic column 4 and the three-way connection 2 or four-way connection that are mentioned below
Outlet be connected, the entrance of mass spectrometer detector is then stretched out from opening 5011 and extended in the tail portion 402 of chromatographic column 4.Using this
Chromatography column holder with accommodation groove 501, no matter chromatographic column how long can all deposit in an orderly manner accommodation groove 501 after coiling
It is interior.
Since the external structure of chromatographic column 4 is the quartz capillary with toughness and hardness, toughness can make chromatography
The coiling bending of column 4, but hardness again limits the degree of the coiling bending of chromatographic column 4, therefore, as a preferred method, such as Fig. 3
Shown, accommodation groove 501 can be oval, so that chromatographic column 4 is easier to store and does not allow frangibility.
For the compactedness of structure, the cross recess for placing three-way connection 2 or four-way connection is additionally provided on ontology 5
502, as shown in figure 3, cross recess 502 is connected with accommodation groove 501, the head 401 of chromatographic column 4 be connected to three-way connection 2 or
The outlet of four-way connection.
If placing three-way connection 2 in the cross recess 502, two imports 201,202 of three-way connection 2 are separately connected from liquid phase
The DC voltage connector of the mobile phase pipeline that chromatograph extracts and ion source, outlet 203 then connect the head of chromatographic column 4
401.The working principle of the three-way connection 2 an are as follows: import of the mobile phase through three-way connection 2 to come is conveyed from liquid chromatograph
201 enter inside three-way connection 2, and DC voltage is entered inside three-way connection 2 by another import 202 of three-way connection 2, will
Compound in mobile phase and mobile phase is ionized, and the mobile phase after ionization enters chromatographic column through the outlet 203 of three-way connection 2
4。
If placing four-way connection in the cross recess 502, two imports of four-way connection, which are separately connected from liquid chromatograph, draws
The DC voltage connector of mobile phase pipeline and ion source out, the head of the one outlet connection chromatographic column 4 of the four-way connection
Portion 401, another outlet are connected by pipeline with waist valve.The working principle of the four-way connection are as follows:, should when waist valve closure
Four-way connection is identical as the working principle of above-mentioned three-way connection 2, and details are not described herein again；When waist valve is opened, from liquid chromatogram
An import of the mobile phase through four-way connection that instrument conveying comes enters inside four-way connection, and DC voltage is by the another of four-way connection
One import enters inside four-way connection, and the compound in mobile phase and mobile phase is ionized, the mobile phase after ionization
One outlet through four-way connection flows out and enters waste liquid bottle through waist valve, without entering chromatographic column 4.
Since chromatographic column 4 is chronically exposed in outside air, the barrel temperatures of chromatographic column 4 can with the change of environment temperature and
It fluctuates and Temperature Distribution is also uneven, seriously reduce the splitter effect of chromatographic column 4.As shown in Fig. 3~Fig. 5, in order to make chromatography
The barrel temperatures of column 4 are stable and are evenly distributed, and can offer the first blind hole 503 for heating rod insertion in the lower part of ontology 5, add
Hot pin can be inserted into first blind hole 503 and heat to the ontology 5, and then protect to the chromatographic column 4 being placed in accommodation groove 501
Temperature, the barrel temperatures to ensure chromatographic column 4 are stable and are evenly distributed.
It further,, can also be in ontology 5 in order to preferably control the barrel temperatures of chromatographic column 4 with continued reference to Fig. 3~Fig. 5
Lower part offer for temp probe insertion the second blind hole 504.Above-mentioned temp probe and heating rod are connected with temperature controller,
Wherein, temp probe can measure the internal temperature of ontology 5, reflect that the temperature of the chromatographic column 4 in accommodation groove 501, temperature are visited indirectly
By the temperature feedback measured to temperature controller, which opens head further according to the temperature measured control heating rod
It closes.Preferably, thermocouple can be selected in temp probe.
Ontology 5 is securable in ion source, and as an implementation, ontology 5 is securable to be previously mentioned in the prior art
Round bar 1 on, referring to Fig. 6, round bar 1 is fixed in ion source, then ontology 5 is securable on the round bar 1.
Since one end of round bar 1 in the prior art is fixed on the fixed part 3 of ion source, ontology 5 can be sheathed on circle
It on bar 1 and is fixed on fixed part 3, to guarantee the stability of the placement of ontology 5, is further ensured that chromatographic column 4 can steadily be set
In in accommodation groove 501.Specifically, referring to Fig. 6 and in conjunction with Fig. 3~Fig. 5, the lower part of ontology 5 offers the through-hole worn for round bar 1
505, while it being also provided with the trough with straight angle 506 matched with the fixed part 3 of rectangular in cross-section, the trough with straight angle 506 and 505 phase of through-hole
Connection.
The ontology 5 of chromatography column holder of the invention is equipped with the accommodation groove 501 for accommodating chromatographic column 4, different length
Chromatographic column 4 can be placed in the accommodation groove 501 after coiling, and avoided chromatographic column 4 and put unordered and be easy to be risk of damage to.Separately
Outside, the second blind hole 504 that the lower part of ontology 5 is equipped with the first blind hole 503 be inserted into for heating rod and is inserted into for temp probe, can be with
It realizes the heating to chromatographic column 4 and temperature controlling function, chromatographic column 4 isolated column effect, stability and reproducibility can not only be improved, also
It can be avoided replacement ion source, save the cost.
More than, schematic description only of the invention, it will be recognized by those skilled in the art that without departing from work of the invention
On the basis of making principle, a variety of improvement can be made to the present invention, this is all belonged to the scope of protection of the present invention.
Claims (5)
1. a kind of chromatography column holder, which is characterized in that including ontology, the ontology is equipped with the appearance for accommodating the chromatographic column
Slot and the cross recess for placing three-way connection or four-way connection are set, the accommodation groove is connected with cross recess, the accommodating
Slot has the opening that the tail portion for the chromatographic column is stretched out.
2. chromatography column holder as described in claim 1, which is characterized in that the accommodation groove is oval.
3. chromatography column holder as described in claim 1, which is characterized in that the lower part of the ontology is offered to be inserted into for heating rod
The first blind hole.
4. chromatography column holder as claimed in claim 3, which is characterized in that the lower part of the ontology is offered inserts for temp probe
The second blind hole entered.
5. chromatography column holder as described in claim 1, which is characterized in that the ontology is fixed in ion source.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710556923.9A CN109239245A (en) | 2017-07-10 | 2017-07-10 | A kind of chromatography column holder |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710556923.9A CN109239245A (en) | 2017-07-10 | 2017-07-10 | A kind of chromatography column holder |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109239245A true CN109239245A (en) | 2019-01-18 |
Family
ID=65083364
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710556923.9A Pending CN109239245A (en) | 2017-07-10 | 2017-07-10 | A kind of chromatography column holder |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109239245A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111239309A (en) * | 2020-01-20 | 2020-06-05 | 安徽省先锋制药有限公司 | High performance liquid chromatography column |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0985928A1 (en) * | 1998-09-08 | 2000-03-15 | SGT Exploitatie B.V. | Assembly for placing a capillary gas chromatography column in a gas chromatograph |
| CN201130173Y (en) * | 2007-12-24 | 2008-10-08 | 上海神开石油化工装备股份有限公司 | Colour spectrum apparatus |
| CN101762661A (en) * | 2010-01-28 | 2010-06-30 | 靳斌 | Chromatographic column box and gas chromatographer thereof |
| CN102095824A (en) * | 2011-01-30 | 2011-06-15 | 上海精密科学仪器有限公司 | Heating device on capillary chromatographic column |
| CN104874202A (en) * | 2015-06-09 | 2015-09-02 | 常州三泰科技有限公司 | Convenient-to-control chromatographic column frame |
| CN106345141A (en) * | 2016-08-25 | 2017-01-25 | 中科生物制药股份有限公司 | Chromatographic column rack |
| CN207051253U (en) * | 2017-07-10 | 2018-02-27 | 中国科学院上海药物研究所 | A kind of chromatogram column holder |
-
2017
- 2017-07-10 CN CN201710556923.9A patent/CN109239245A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0985928A1 (en) * | 1998-09-08 | 2000-03-15 | SGT Exploitatie B.V. | Assembly for placing a capillary gas chromatography column in a gas chromatograph |
| CN201130173Y (en) * | 2007-12-24 | 2008-10-08 | 上海神开石油化工装备股份有限公司 | Colour spectrum apparatus |
| CN101762661A (en) * | 2010-01-28 | 2010-06-30 | 靳斌 | Chromatographic column box and gas chromatographer thereof |
| CN102095824A (en) * | 2011-01-30 | 2011-06-15 | 上海精密科学仪器有限公司 | Heating device on capillary chromatographic column |
| CN104874202A (en) * | 2015-06-09 | 2015-09-02 | 常州三泰科技有限公司 | Convenient-to-control chromatographic column frame |
| CN106345141A (en) * | 2016-08-25 | 2017-01-25 | 中科生物制药股份有限公司 | Chromatographic column rack |
| CN207051253U (en) * | 2017-07-10 | 2018-02-27 | 中国科学院上海药物研究所 | A kind of chromatogram column holder |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111239309A (en) * | 2020-01-20 | 2020-06-05 | 安徽省先锋制药有限公司 | High performance liquid chromatography column |
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