CN109232687A - A kind of extracting method of Chinese herbaceous peony plant different parts paeoniflorin content - Google Patents
A kind of extracting method of Chinese herbaceous peony plant different parts paeoniflorin content Download PDFInfo
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- CN109232687A CN109232687A CN201811174201.8A CN201811174201A CN109232687A CN 109232687 A CN109232687 A CN 109232687A CN 201811174201 A CN201811174201 A CN 201811174201A CN 109232687 A CN109232687 A CN 109232687A
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- herbaceous peony
- elution
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H17/00—Compounds containing heterocyclic radicals directly attached to hetero atoms of saccharide radicals
- C07H17/04—Heterocyclic radicals containing only oxygen as ring hetero atoms
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H1/00—Processes for the preparation of sugar derivatives
- C07H1/06—Separation; Purification
- C07H1/08—Separation; Purification from natural products
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Abstract
The invention discloses a kind of extracting method of Chinese herbaceous peony plant different parts paeoniflorin content, including materials, elution, separation, drying, crushing, sieving, enzymatic hydrolysis, centrifugal filtration, it is sliced, boils, filters and elutes refining.Elution is all made of distilled water with enzymolysis process and is operated, without the use of other solvents other than system components, to guarantee that new impurity will not be introduced, and then guarantee the precision extracted, dilute hydrochloric acid solution is added in enzymolysis process, so that raw material cell wall ruptures, effective component in raw material is more extracted sufficiently and more easily, unsifted bulky grain carries out separating twice, make Chinese herbaceous peony plant obtain floral leaf part adequately to be crushed, the filter residue filtered out carries out secondary boil, in order to which the Paeoniflorin contained in rhizome can be extracted completely, elution process is first with distillation water elution impurity, the ethanol elution of 70-80% is used again, finally collect ethanol eluate, ensure the purity of extracted Paeoniflorin.
Description
Technical field
The present invention relates to a kind of extracting method, specially a kind of extraction side of Chinese herbaceous peony plant different parts paeoniflorin content
Method belongs to organic compound extractive technique field.
Background technique
Chinese herbaceous peony is that Paeoniaceae Paeonia herbaceos perennial has very in the cultivation history that China has more than 4000 years
High ornamental value and medical value, and Paeoniflorin is the main effective of a variety of parts of generic medicinal plants such as Radix Paeoniae Alba, radix paeoniae rubra and cortex moutan
Ingredient has significant ground antalgic and sedative anticonvulsant action, and energy coronary artery dilator increases coronary flow, and confrontation Acute myocardial lacks
Blood reduces blood pressure, protection cerebral ischemia, antithrombus formation, platelet aggregation-against, and lipidemia reverses the effect of low blood pressure, Anhui
Bozhou area is Radix Paeoniae Alba Genuine producing area, high-quality and well-known throughout the country because its yield is big, the harvesting of existing Radix Paeoniae Alba with 3-5 life be it is more,
Some Enterprises are that the Paeoniflorin monomer extracted in Radix Paeoniae Alba is used as raw material.
The existing method for extracting Paeoniflorin has alcohol solution reflux extraction, CO2 supercritical fluid extraction, UF membrane
Method etc., although these prior arts have attempted a variety of different extractions and purification process, some method and processes are complicated, some need
Special or complicated equipment, but since these prior arts can not completely destroy the constraint of cell wall, it mentions to the maximum extent
Effective component is taken out, and sufficiently rationally separation is carried out to active principle, thus products obtained therefrom purity is not high, is also not easy scale metaplasia
It produces.
Summary of the invention
Contain the object of the invention is that providing a kind of Chinese herbaceous peony plant different parts Paeoniflorin to solve the above-mentioned problems
The extracting method of amount.
The present invention is through the following technical solutions to achieve the above objectives: a kind of Chinese herbaceous peony plant different parts paeoniflorin content
Extracting method, extracting method include the following steps
Step A: materials choose the Chinese herbaceous peony plant of different Growing years；
Step B: elution will be attached to the removal of the impurity such as the soil on Chinese herbaceous peony plant surface；
Step C: separation separates each section flower of Chinese herbaceous peony plant, leaf, stem and root；
Step D: it is dry, floral leaf isolated etc. is partially disposed in baking oven in 105 DEG C of water-removing 10min, then at 70 DEG C
It is lower that drying to constant weight；
Step E: it crushes, is crushed the floral leaf after drying using 400Y type pulverizer；
Step F: sieving is screened smashed raw material using 40 meshes；
Step G: enzymatic hydrolysis pours into the floral leaf powder of sieving in the complex enzyme formulation buffer of activation, is 40-60 in temperature
DEG C water-bath on digest 3-15h；
Step H: centrifugal filtration is centrifuged using 5810R type centrifuge, isolates Paeoniflorin liquid；
Step I: rhizome portion is cut flakiness using Leica EM UC6 type ultramicrotome by slice；
Step J: boiling, and the rhizome thinly sliced pours into the water that weight ratio is 1:15, decocts 50min；
Step K: filtering, the liquid boiled are separated filtrate with filter residue by strainer；
Step L: filtered filtrate is concentrated for elution refining, and upper large pore resin absorption column is eluted, eluent heating
Afterwards plus active carbon decolorization filtering, filtrate, which is further concentrated to no alcohol, can obtain Paeoniflorin crude product, and crude product adds ethyl acetate crystallisation by cooling, mistake again
Filter, crystal is dry to get product.
Preferably, in order to guarantee the precision extracted, the elution of the step B is all made of steaming with the enzymolysis process of step G
Distilled water is operated.
Preferably, in order to keep the effective component in raw material more abundant and more easily be extracted, the step G enzymatic hydrolysis
Dilute hydrochloric acid solution is added in process, adjusts Ph value to 3-6, is then digested.
Preferably, it is adequately crushed to make Chinese herbaceous peony plant obtain floral leaf part, unsifted big in the step F
Grain return step E carries out separating twice.
Preferably, in order to extract the Paeoniflorin contained in rhizome completely, the filter that is filtered out in the step K
Slag enters step J and carries out secondary boil.
Preferably, in order to ensure the purity of extracted Paeoniflorin, the elution process of the step L is first with distillation water elution
Impurity, then with the ethanol elution of 70-80%, finally collect ethanol eluate.
The beneficial effects of the present invention are: the extracting method design of the Chinese herbaceous peony plant different parts paeoniflorin content is rationally, step
The rapid elution of B and the enzymolysis process of step G are all made of distilled water and are operated, without the use of other molten other than system components
Agent to guarantee that new impurity will not be introduced, and then guarantees the precision extracted, and dilute hydrochloric acid solution is added in step G enzymolysis process,
Ph value is adjusted to 3-6, is then digested, so that raw material cell wall ruptures, the effective component in raw material more sufficiently and is easier
Ground is extracted, and unsifted bulky grain return step E carries out separating twice in step F, and Chinese herbaceous peony plant is made to obtain floral leaf part
Adequately crushed, avoid wastage of material, the filter residue filtered out in step K enter step J carry out it is secondary boil, by multiple
It boils, in order to be able to extract the Paeoniflorin contained in rhizome completely, the elution process of step L is first with distillation water elution
Impurity, then with the ethanol elution of 70-80%, ethanol eluate is finally collected, make the more thorough of elution, it is ensured that extracted Chinese herbaceous peony
The purity of medicine glycosides.
Detailed description of the invention
Fig. 1 is schematic structural view of the invention.
Specific embodiment
Following will be combined with the drawings in the embodiments of the present invention, and technical solution in the embodiment of the present invention carries out clear, complete
Site preparation description, it is clear that described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.It is based on
Embodiment in the present invention, it is obtained by those of ordinary skill in the art without making creative efforts every other
Embodiment shall fall within the protection scope of the present invention.
Referring to Fig. 1, a kind of extracting method of Chinese herbaceous peony plant different parts paeoniflorin content, extracting method includes following
Step
Step A: materials choose the Chinese herbaceous peony plant of different Growing years；
Step B: elution will be attached to the removal of the impurity such as the soil on Chinese herbaceous peony plant surface；
Step C: separation separates each section flower of Chinese herbaceous peony plant, leaf, stem and root；
Step D: it is dry, floral leaf isolated etc. is partially disposed in baking oven in 105 DEG C of water-removing 10min, then at 70 DEG C
It is lower that drying to constant weight；
Step E: it crushes, is crushed the floral leaf after drying using 400Y type pulverizer；
Step F: sieving is screened smashed raw material using 40 meshes；
Step G: enzymatic hydrolysis pours into the floral leaf powder of sieving in the complex enzyme formulation buffer of activation, is 40-60 in temperature
DEG C water-bath on digest 3-15h；
Step H: centrifugal filtration is centrifuged using 5810R type centrifuge, isolates Paeoniflorin liquid；
Step I: rhizome portion is cut flakiness using Leica EM UC6 type ultramicrotome by slice；
Step J: boiling, and the rhizome thinly sliced pours into the water that weight ratio is 1:15, decocts 50min；
Step K: filtering, the liquid boiled are separated filtrate with filter residue by strainer；
Step L: filtered filtrate is concentrated for elution refining, and upper large pore resin absorption column is eluted, eluent heating
Afterwards plus active carbon decolorization filtering, filtrate, which is further concentrated to no alcohol, can obtain Paeoniflorin crude product, and crude product adds ethyl acetate crystallisation by cooling, mistake again
Filter, crystal is dry to get product.
The elution of the step B and the enzymolysis process of step G are all made of distilled water and are operated, and are without the use of system components
Other solvents in addition to guarantee that new impurity will not be introduced, and then guarantee that the precision extracted, the step G digested
Dilute hydrochloric acid solution is added in journey, adjusts Ph value to 3-6, is then digested so that raw material cell wall ruptures, in raw material it is effective at
Dividing more abundant and is more easily extracted, unsifted bulky grain return step E carries out separating twice in the step F,
Make Chinese herbaceous peony plant obtain floral leaf part adequately to be crushed, avoids wastage of material, the filter residue filtered out in the step K enters step
Rapid J progress is secondary to boil, by repeatedly boiling, in order to be able to the Paeoniflorin contained in rhizome is extracted completely, it is described
The elution process of step L is first with distillation water elution impurity, then with the ethanol elution of 70-80%, finally collects ethanol eluate, make
What is eluted is more thorough, it is ensured that the purity of extracted Paeoniflorin.
It is obvious to a person skilled in the art that invention is not limited to the details of the above exemplary embodiments, Er Qie
In the case where without departing substantially from spirit or essential attributes of the invention, the present invention can be realized in other specific forms.Therefore, no matter
From the point of view of which point, the present embodiments are to be considered as illustrative and not restrictive, and the scope of the present invention is by appended power
Benefit requires rather than above description limits, it is intended that all by what is fallen within the meaning and scope of the equivalent elements of the claims
Variation is included within the present invention.Any reference signs in the claims should not be construed as limiting the involved claims.
In addition, it should be understood that although this specification is described in terms of embodiments, but not each embodiment is only wrapped
Containing an independent technical solution, this description of the specification is merely for the sake of clarity, and those skilled in the art should
It considers the specification as a whole, the technical solutions in the various embodiments may also be suitably combined, forms those skilled in the art
The other embodiments being understood that.
Claims (6)
1. a kind of extracting method of Chinese herbaceous peony plant different parts paeoniflorin content, it is characterised in that: its extracting method includes following
Step
Step A: materials choose the Chinese herbaceous peony plant of different Growing years；
Step B: elution will be attached to the removal of the impurity such as the soil on Chinese herbaceous peony plant surface；
Step C: separation separates each section flower of Chinese herbaceous peony plant, leaf, stem and root；
Step D: it is dry, floral leaf isolated etc. is partially disposed in baking oven in 105 DEG C of water-removing 10min, is then dried at 70 DEG C
It does to constant weight；
Step E: it crushes, is crushed the floral leaf after drying using 400Y type pulverizer；
Step F: sieving is screened smashed raw material using 40 meshes；
Step G: enzymatic hydrolysis pours into the floral leaf powder of sieving in the complex enzyme formulation buffer of activation, is 40-60 DEG C in temperature
3-15h is digested in water-bath；
Step H: centrifugal filtration is centrifuged using 5810R type centrifuge, isolates Paeoniflorin liquid；
Step I: rhizome portion is cut flakiness using Leica EM UC6 type ultramicrotome by slice；
Step J: boiling, and the rhizome thinly sliced pours into the water that weight ratio is 1:15, decocts 50min；
Step K: filtering, the liquid boiled are separated filtrate with filter residue by strainer；
Step L: filtered filtrate is concentrated for elution refining, and upper large pore resin absorption column is eluted, and adds after eluent heating
Active carbon decolorization filtering, filtrate, which is further concentrated to no alcohol, can obtain Paeoniflorin crude product, and crude product adds ethyl acetate crystallisation by cooling again, filtering,
Crystal is dry to get product.
2. a kind of extracting method of Chinese herbaceous peony plant different parts paeoniflorin content according to claim 1, it is characterised in that:
The elution of the step B and the enzymolysis process of step G are all made of distilled water and are operated.
3. a kind of extracting method of Chinese herbaceous peony plant different parts paeoniflorin content according to claim 1, it is characterised in that:
Dilute hydrochloric acid solution is added in the step G enzymolysis process, adjusts Ph value to 3-6, is then digested.
4. a kind of extracting method of Chinese herbaceous peony plant different parts paeoniflorin content according to claim 1, it is characterised in that:
Unsifted bulky grain return step E carries out separating twice in the step F.
5. a kind of extracting method of Chinese herbaceous peony plant different parts paeoniflorin content according to claim 1, it is characterised in that:
The filter residue filtered out in the step K enters step J and carries out secondary boil.
6. a kind of extracting method of Chinese herbaceous peony plant different parts paeoniflorin content according to claim 1, it is characterised in that:
The elution process of the step L is first with distillation water elution impurity, then with the ethanol elution of 70-80%, finally collects ethanol elution
Liquid.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114891564A (en) * | 2022-04-18 | 2022-08-12 | 张朝风 | Method for extracting and preparing plant natural perfume |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101062128A (en) * | 2007-06-18 | 2007-10-31 | 石任兵 | Extract of total glucosides of paeony and the preparing method thereof |
| CN101628926A (en) * | 2009-08-11 | 2010-01-20 | 张守力 | Extraction process of paeoniflorin |
| CN101875676A (en) * | 2010-07-02 | 2010-11-03 | 西北农林科技大学 | Method for extracting paeoniflorin from white paeony root by enzyme process |
| CN102372750A (en) * | 2010-08-10 | 2012-03-14 | 张作光 | Method for simultaneously preparing albiflorin and paeoniflorin |
| CN103130850A (en) * | 2013-02-27 | 2013-06-05 | 河南科技大学 | Method for preparing paeoniflorin from oil peony seed meal |
-
2018
- 2018-10-09 CN CN201811174201.8A patent/CN109232687A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101062128A (en) * | 2007-06-18 | 2007-10-31 | 石任兵 | Extract of total glucosides of paeony and the preparing method thereof |
| CN101628926A (en) * | 2009-08-11 | 2010-01-20 | 张守力 | Extraction process of paeoniflorin |
| CN101875676A (en) * | 2010-07-02 | 2010-11-03 | 西北农林科技大学 | Method for extracting paeoniflorin from white paeony root by enzyme process |
| CN102372750A (en) * | 2010-08-10 | 2012-03-14 | 张作光 | Method for simultaneously preparing albiflorin and paeoniflorin |
| CN103130850A (en) * | 2013-02-27 | 2013-06-05 | 河南科技大学 | Method for preparing paeoniflorin from oil peony seed meal |
Non-Patent Citations (5)
| Title |
|---|
| 吴雄壮 等: "不同部位和不同生长年限白芍中的芍药苷含量测定", 《中国现代应用药学杂志》 * |
| 孟祥松 等: "芍药植株不同部位芍药苷含量分析", 《安徽医药》 * |
| 张新图: "赤、白芍不同部位中芍药苷和苯甲酸的含量差异", 《实用中医内科杂志》 * |
| 梁剑平: "《兽医中药学及实验技术》", 31 December 2012, 军事医学科学出版社 * |
| 简在友 等: "芍药不同部位和不同采收期6个化学活性成分含量的比较", 《药学学报》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114891564A (en) * | 2022-04-18 | 2022-08-12 | 张朝风 | Method for extracting and preparing plant natural perfume |
| CN114891564B (en) * | 2022-04-18 | 2024-01-30 | 吴基 | Method for extracting and preparing plant natural spice |
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