CN109223904A - A kind of Bushen Huoxue Fang and preparation method thereof - Google Patents
A kind of Bushen Huoxue Fang and preparation method thereof Download PDFInfo
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Abstract
The present invention relates to the field of Chinese medicines, and in particular to a kind of Chinese materia medica preparation and preparation method thereof containing resveratrol.General Chinese materia medica preparation is water decoction, the effective component in medicinal material cannot effectively be extracted, and for the prescription of many treatment nephrosis and anti-aging significant in efficacy, the height of the Resveratrol content in compound has direct relation with curative effect, and the solubility of resveratrol in water is smaller.Therefore the present invention provides a kind of Bushen Huoxue Fangs, by the following Chinese medicinal composition calculated by weight: 5-15 parts of fructus lycii, 4-8 parts of polygonum cuspidate, 5-15 parts of mulberries, 5-15 parts of Radix Angelicae Sinensis, 2-6 parts of Radix Notoginseng；And preparation method thereof: medicinal material is pre-processed, Chinese medical concrete is made in alcohol extracting.Bushen Huoxue Fang has that tonifying speen and tonifying kidney, promoting blood circulationization be turbid, anti-aging and other effects；Resveratrol therein can be farthest extracted with the technique that ethyl alcohol extracts, improves the effective component of prescription, optimizes therapeutic effect.
Description
Technical field
The present invention relates to the field of Chinese medicines, are more particularly to a kind of Chinese materia medica preparation and preparation method thereof containing resveratrol.
Background technique
Resveratrol (Resveratrol, abbreviation Res) is the polyphenolic substance of non-flavonoids, and chemical name is (E) 1,
5,4 one trihydroxy talan, molecular formula C14H1203, relative molecular mass 228.25g/mol is white needle-like crystals,
It is present in many plants, (Song Hainan, money are kind for a kind of phytoalexin generated when being plant by biology or abiotic stress
Army, the extraction of Liu Wei Resveratrol in Rhizoma Polygoni Cuspidati and analysis and detection technology progress [J] Chemical Industry in Guangzhou, 2014,42 (21):
18-19.).The resveratrol of high dose has the function of anti-inflammatory, removing free radical, antibacterial and anti-cancer in vivo.It is early in China
The Chinese medicine polygonum cuspidate containing resveratrol has been used to have been used for treating and preventing hyperlipidemia, artery sclerosis.Meanwhile resveratrol can be with
Protective effect is played to angiocarpy from many aspects.
There is experiment and show that Res has good anti-aging, antioxidant ability of organism can be improved, improve testis
The function of endocrine cell, the recovery for promoting spermatogenesis, the decline for delaying sexual gland function.And it is small to improve aging male
Mouse sperm quality is (as increased sperm concentration, motility rate and vigor；And reduce defective sperm ratio etc.), and improve Main Reproductive Organs
The Seminiferous Epithelium Structure and function of (testis) are so as to improve senile organism reproductive function.Separately there is many experiments evidence to show white black false hellebore
Alcohol has anti-aging, antitumor, anti-inflammatory and immunoregulatory effect, and aged mouse and the immune function of people can be improved.
General Chinese materia medica preparation is all water decoction, i.e., the prepared slices of Chinese crude drugs is placed in boiling water and is decocted, and patient takes the extraction of its water
Product.But for the prescription of many treatment nephrosis and anti-aging significant in efficacy, the height of the Resveratrol content in compound
It is low to have direct relation with curative effect.However the solubility of resveratrol in water is lower, therefore decoction cannot fully extract Chinese medicine
Resveratrol in material.Therefore, traditional traditional Chinese medical theory is combined with modern Natural products research method, develops one
Kind improves the preparation method of the Resveratrol content in the prescription for the treatment of nephrosis and anti-aging, before having good production application
Scape, and have very important significance for developing with inheriting tradition TCM Culture.
Summary of the invention
General Chinese materia medica preparation is all water decoction, i.e., the prepared slices of Chinese crude drugs is placed in boiling water and is decocted, and patient takes its extract.
But for the prescription of many treatment nephrosis and anti-aging significant in efficacy, the height of the Resveratrol content in compound and treatment
Effect has direct relation.However the solubility of resveratrol in water is lower, therefore during general water decoction cannot extract fully
Resveratrol in medicinal material.For this purpose, the present invention provides a kind of Bushen Huoxue Fang, by the following Chinese medicine group calculated by weight
At: 5-15 parts of fructus lycii, 4-8 parts of polygonum cuspidate, 5-15 parts of mulberries, 5-15 parts of Radix Angelicae Sinensis, 2-6 parts of Radix Notoginseng.
A kind of Bushen Huoxue Fang, by the following Chinese medicinal composition calculated by weight: 10 parts of fructus lycii, 6 parts of polygonum cuspidate, mulberries 10
Part, 10 parts of Radix Angelicae Sinensis, 4 parts of Radix Notoginseng.
A kind of Bushen Huoxue Fang, preparation method are as follows:
(1) fructus lycii, polygonum cuspidate, mulberries, Radix Angelicae Sinensis and Radix Notoginseng are weighed by weight, and medicinal material is pre-processed；
(2) pretreated medicinal material adds the 30-60% ethyl alcohol of 4-10 times of medicinal material amount one step up, impregnates 1-21 days, medical filtration
After be centrifuged, take supernatant；
(3) the resulting supernatant of previous step is placed in evaporating dish, rotation is evaporated, pressure 30-50Pka, 30-70 DEG C of temperature, is turned
Fast 60-90 r/min takes out, and sets and cools down 10-60 min in dry vessel to get Chinese medical concrete.
The preprocess method includes that medicine materical crude slice or crushing is made.
The invention has the benefit that the clinical efficacy of Chinese materia medica preparation disclosed by the invention includes: tonifying speen and tonifying kidney, promoting blood circulationization
Premature ovarian failure and treatment pain in the loins etc. caused by turbid, anti-aging, the treatment for facilitating nephrosis, prevention and treatment Tripterygium Preparations.With the present invention
The Chinese materia medica preparation of the preparation method preparation of announcement, the content of resveratrol significantly improves, the solubility of resveratrol in water compared with
It is small, resveratrol therein can be farthest extracted with the technique that ethyl alcohol extracts, improves the effective component of prescription, is optimized
Therapeutic effect.Preparation method is simple, and the medicinal extract being eventually fabricated facilitates patient to take.
Detailed description of the invention
The HPLC map of Fig. 1 reference substance solution.
The HPLC map of Fig. 2 sample solution.
The HPLC map of Fig. 3 negative sample.
Specific embodiment
A kind of Bushen Huoxue Fang, by the following Chinese medicinal composition calculated by weight: 5-15 parts of fructus lycii, 4-8 parts of polygonum cuspidate, mulberry
5-15 parts of Shen, 5-15 parts of Radix Angelicae Sinensis, 2-6 parts of Radix Notoginseng.
Preference: 10 parts of fructus lycii, 6 parts of polygonum cuspidate, 10 parts of mulberries, 10 parts of Radix Angelicae Sinensis, 4 parts of Radix Notoginseng.
Preparation method is as follows:
(1) fructus lycii, polygonum cuspidate, mulberries, Radix Angelicae Sinensis and Radix Notoginseng are weighed by weight, and medicinal material is pre-processed.
(2) pretreated medicinal material adds the 30-60% ethyl alcohol of 4-10 times of medicinal material amount one step up, impregnates 1-21 days, medical fluid
It is centrifuged after filtering, takes supernatant.
(3) the resulting supernatant of previous step is placed in evaporating dish, rotation is evaporated, pressure 30-50Pka, temperature 30-70
DEG C, revolving speed 60-90 r/min；It takes out, sets and cool down 10-60 min in dry vessel to get Chinese medical concrete.
The preprocess method includes that medicine materical crude slice or crushing is made.
Analysis condition and method:
1.HPLC condition: pre-column (phenomenex-KJ0-4282)；Chromatographic column: phenomenex-C18(250 × 4.6 mm, 5
μm)；Mobile phase: -0.1% phosphate aqueous solution (B) (28: 72) of acetonitrile (A), elution requirement: 0.01 ~ 8.50 min, 28%A；8.51~
14.00 min, 28 % ~ 40 %A；14.01 ~ 20.00 min, 90 %A；20.10 ~ 28.00 min, 28 %A；Flow velocity: 0.01 ~
5.00 min, 1.0 ml/mim；5.01 ~ 5.50 min, 1.0 ml/min ~ 0.8 ml/min；5.50 ~ 9.00 min, 0.8 ml/
min；9.01 ~ 9.50 min, 0.8 ~ 1.0 ml/min；9.51 ~ 28.00 min, 1.0 ml/min；Column temperature: 30 DEG C；Detect wave
It is long: 310 nm；Sample volume: 5 μ l.
2. the standard curve preparation of resveratrol in medicinal material pre-treatment step: precision draws resveratrol reference substance solution
(61.628 μ g/ml) 1 μ l, 2 μ l, 3 μ l, 5 μ l, 10 μ l are dissolved in methanol solution, are settled to 10mL: by the detection of above-mentioned HPLC
Method is detected, and the integrated value of chromatographic peak area is recorded, with resveratrol sample volume (μ g) be abscissa (X), to measure peak
Area integral value be ordinate (Y), draw standard curve.Regression equation isY = (7.81425×106)X, (r=0.9999, inspection
Survey range: 0.061 628-0.616,28 μ g/ml).
3. the preparation of the standard curve of resveratrol in medicinal extract: it is appropriate that precision weighs resveratrol reference substance, adds methanol molten
Solution, is made the reference substance stock solution that mass concentration is 0.234 mg/ml, and control series product solution (white black false hellebore is made in proportional diluted
Determining alcohol is followed successively by 0.234,0.117,0.058 5,0.029 25,0.014 63,0.0073 125,0.0036 563 mg/
Ml).Precision measures above-mentioned each 5 μ l of control series product solution, detects according to above-mentioned HPLC method, obtains each concentration samples
Peak area draws standard curve.Regression equation isY=3.92×107 X- 55939.2(r=0.9999, detection range are as follows: 36.56 ~
234.00 μ g/ml).
4. prepared by reference substance (A) solution: resveratrol reference substance, concentration are 0.234 mg/ml.
5. the preparation of sample solution (B):
Fructus lycii 10g, mulberries 10g, polygonum cuspidate 6g, Radix Angelicae Sinensis 10g, Radix Notoginseng 4g are taken, 320mL55% ethanol solution, leaching is added in grinds
Bubble 14 days takes ethanol extract supernatant, and 45000 r/min are centrifuged 10 min, take supernatant, is settled to 250 ml, and precision is inhaled
10 ml are taken, rotation is evaporated (pressure: 40Pka；Temperature: 50 DEG C；Revolving speed: 75 r/min), residue add -0.1% phosphoric acid of acetonitrile (28:
72) solution is settled to 10 ml, through 0.45 μm of filtering with microporous membrane, take subsequent filtrate to get.
6. prepared by negative sample (C) solution:
Fructus lycii 10g, mulberries 10g, Radix Angelicae Sinensis 10g, Radix Notoginseng 4g are taken, grinds are impregnated 14 days with 55% ethyl alcohol.Ethanol extract is taken,
45000 r/min are centrifuged 10 min, take supernatant, are settled to 250 mL, and precision draws 10 mL, rotation be evaporated (pressure:
40Pka；Temperature: 50 DEG C；Revolving speed: 75 r/min), residue adds acetonitrile -0.1 % phosphoric acid (28: 72) solution to be settled to 10 ml, warp
0.45 μm of filtering with microporous membrane, take subsequent filtrate to get.
7. the calculation method of dry cream rate:
Precision draws 25.0 mL of test solution, sets in the evaporating dish of constant weight, water bath method, in 105 DEG C of dry 3 h, takes out,
Set in drier cooling 30 min, accurately weighed quality, according to formula (paste-forming rate=dry cream quality × 10/ quality of medicinal material ×
It 100%) is dry cream rate.
Experimental section:
1. influence of the medicinal material preprocess method to Resveratrol content.
The first preprocess method: taking 2 parts of recipe quantity medicine materical crude slice, respectively plus 5 times of yellow rice wine of recipe quantity, impregnates 1 month.Medical fluid mistake
It is centrifuged after filter, supernatant is taken to be settled to 200ml with yellow rice wine, shaken up to get medicine materical crude slice -1, medicine materical crude slice -2 is remembered.
Second of preprocess method takes 2 parts of recipe quantity medicinal powder, wraps up medicinal powder with gauze respectively, adds 5 times of yellow rice wine of recipe quantity,
It impregnates 1 month.It is centrifuged after medical filtration, supernatant is taken to be settled to 200ml with yellow rice wine, shaken up to get powder -1, powder -2 is remembered.
The total content of the resveratrol of -1 different pretreatments method of table.
The above results indicate that the Resveratrol content that pulverizing medicinal materials are obtained is more preferable, therefore below in an example, it is right
Medicinal material carries out comminution pretreatment.
2. influence of the Different Preparation to Resveratrol content.
Above-mentioned reference substance solution (A), sample solution (B) and negative sample (C) are examined according to above-mentioned HPLC detection method
It surveys, the HPLC map obtained is as shown in Fig. 1 to figure:
Resveratrol is primarily present in Polygonum cuspidatum Sieb. et Zucc, therefore, (is lacked in conjunction with resveratrol reference substance and negative controls solution
Polygonum cuspidate this herb) HPLC map, can determine that the peak 1 (t=12.5min) in Fig. 2 is resveratrol peak.
Using the factor of influence resveratrol extracted amount as variable, wherein concentration of alcohol/%(A), impregnate number of days/day
(B), granularity (C), solid-liquid ratio (D), is as follows:
The variable factor of the influence Resveratrol content of table 1.
By L9( 34 ) orthogonal test, the extract obtained under the conditions of the orthogonal test combined with various variables is used
The HPLC analysis method detection stated, obtains the content of wherein resveratrol, using resveratrol, dry cream rate as evaluation index.Knot
Fruit is as shown in the table:
2 L of table9(34) Orthogonal Experiment and Design and result.
Learnt from table 2, be A1(concentration of alcohol 55% in condition), B3(impregnate number of days 21 days), powder in C3() and D3(feed liquid
Under 8), Resveratrol content highest in extract, dry cream rate is also more satisfactory.
Orthogonal experiments are analyzed using SPSS 19.0, the results of analysis of variance is shown in Table 4.By intuitively analyzing it is found that
Each factor acts on primary and secondary to Chinese materia medica preparation alcohol extraction process disclosed by the invention as A concentration of alcohol > D solid-liquid ratio > C granularity > B leaching
Steep number of days；Variance analysis shows that concentration of alcohol (A), solid-liquid ratio (D) have test result and significantly affects (P < 0.05).B leaching
It steeps number of days and the data fitting effect of C granularity is not significant.
The variance analysis of 3 quadrature factor of table.
As known from Table 3, impregnating number of days and granularity does not have conspicuousness for the content of resveratrol；In addition, B impregnates number of days pair
As a result influence very little impregnates 14 days in conjunction with actual production and the time cost difference of immersion 21 days is larger, therefore, choosing is impregnated
It is used as optimal conditions within 14 days.
Fructus lycii: energy help essence various symptims and signs of deficiency, easy color bleach, and improving eyesight is calmed the nerves.
Polygonum cuspidate: there is removing dampness through diuresis and removing jaundice, clearing heat and detoxicating, removing blood stasis and acesodyne, relieving cough and reducing sputum effect.
Mulberries: it is sweet in flavor and cold in property, there is liver-kidney tonifying, fluid dryness, UFA eyesight and other effects.
Radix Angelicae Sinensis: there is the effect of benefiting blood and regulating blood circulation, menstruction regulating and pain relieving, moisturizing dryness and lubricating intestine, anticancer, anti-ageing anti-old, raising are immunized.
Radix Notoginseng: dissipating stasis and stanching bleeding, detumescence ding-tong.
Clinical implementation case:
Case one:
Chinese materia medica preparation disclosed by the invention can be used for premature ovarian failure caused by preventing and treating Tripterygium Preparations.
40 chronic nephritis female patients are chosen in this research, divide treatment group and control group each 20 at random.Treatment group is with thunder
The public more glycosides piece 20mg of rattan are three times a day with Chinese materia medica preparation disclosed by the invention, and control group is with Tripterygium wilfordii Polyglycosidium Tablets 20mg daily three
Secondary treatment assesses two groups of curative effects according to patient clinical symptom grade form, biochemical indicator and menstruation after 3 months, if after 3 months
There is menstrual cycle shortening in patient, menstrual flow is reduced, even amenorrhoea, gives and withdraws Tripterygium wilfordii Polyglycosidium Tablets, and regular follow-up tracks patient
Menstruation recovery situation.Invention formulation can effectively mitigate patients with chronic glomerulonephritis Urine proteins as the result is shown, improve renal function, and alleviation is faced
Bed symptom, delays nephrosis to be in progress.Especially contribute to female ovary hypofunction caused by antagonism Tripterygium Preparations, ovulation failure feelings
Condition.After deactivating Tripterygium Preparations, compare treatment group and control group, it is extensive that Chinese materia medica preparation patient menstrual disclosed by the invention is taken in discovery
The multiple time is fast compared with other patients, and simultaneously in application process, and the ratio for paramenia occur reduces, hormone serum level anomaly ratio
Example is low compared with control group.
Tripterygium wilfordii Polyglycosidium Tablets are widely used in chronic kidney disease and the smelting of panimmunity disease is treated, and than common hormone and exempt from
Epidemic disease inhibitor few side effects, it is particularly possible to avoid making using the puffiness of the eyes caused by hormone, central obesity, blood glucose rise etc. are secondary
With for diabetic nephropathy, asymptomatic proteinuria, a variety of kidney troubles such as IgA nephrosis have good result.Blemish in an otherwise perfect thing
It is that about 30% patient may occur in which that reproductive function is low after taking Tripterygium wilfordii Polyglycosidium Tablets, shows as Hypomenorrhea, amenorrhoea, sexual desire subtract
It moves back, the decline of dysfunctional uterine bleeding, sex hormone level, B ultrasound shows as ovary or metratrophia, womb are less than normally
The performance of equal premature ovarian failures.Although subjects reproductive's hypofunction can slowly improve after some patientss deactivate Tripterygium Preparations,
But still there are some patients to will appear irreversible ovary damage.Regarding to the issue above in clinical application, find in Bushenhuoxue
Medicine has clear improvement the effect of above-mentioned condition.It is observed that taking Chinese materia medica preparation patient disclosed by the invention, Thunder God is being taken
Hypomenorrhea, amenorrhoea, sex hormone level are obviously shortened the time required to reaching normal level compared with control group after the more glycosides pieces of rattan, and ovary
The performance of early ageing is substantially reduced.Chinese materia medica preparation disclosed by the invention can have Ployglycosidium, and turbid function is let out in invigorating the spleen.
Case two:
Treat the observation of renal deficiency type patient clinical:
60 tcm diagnosis of clinical observation are the patient of kidney deficiency, and treatment group 30 gives Chinese medicine preparation treatment disclosed by the invention, right
Liuwei Dihuang Wan treatment is given according to group 30.The course for the treatment of 6 weeks, using patient symptom integral as evaluation criterion.
Note: kidney deficiency CM syndrome differentiation criterion is with reference to the research of nineteen eighty-two whole nation combination of Chinese tradiational and Western medicine asthenic symptoms and prevention and treatment disease of old people meeting: 1. waist
Back is ached, 2. soreness of waist and knee or talagia, 3. Hiccough and deaf, is 4. sent out de- tooth and is shaken, 5. dripping urination or incontinence, 6. impotence, premature ejaculation
Or irregular menstruation.Kidney deficiency can be diagnosed as by having three.
The study find that Chinese materia medica preparation disclosed by the invention improves significantly Patients with Kidney Deficiency Type symptom compared with Liuwei Dihuang Wan, resist
Aging effects are good, and we invigorate the kidney and stop nocturnal emission, and adjust essence in kidney, balance kidney yin kidney yang, make human body vital qi memory, the five internal organs are healthy and vigorous.It can
Can have with it and remove free radical, controlling gene signal, enhancing immune function, repair neuroendocrine disorders, have and delay machine
Senile symptom is alleviated in body aging, and extension gerontal patient's service life acts on.
Case three:
For treating pain in the loins:
It is kidney deficiency for clinical manifestation, the patient of cold-dampness and qi stagnation and blood stasis type pain in the loins is accompanied, above dialectical use (i.e. the side of steeping in wine)
Its pain in the loins symptom can be relieved.Liquid medicine is one of a variety of dosage forms of Chinese medicine, and Huangdi's Internal Classics have " nutritious beverage,thick liquor and sweet liquor discusses a piece ", specially
Door discusses the road of medication." wine with dregs sweet wine " i.e. liquid medicine." length that wine is hundred medicines ", wine are easier to enter in Chinese medicine histocyte, will
Various difference organic substances are dissolved out, to play the original therapeutic effect of crude drug.Liquid medicine is for treating this type pain in the loins, oral administration
It is applied with, easily and effectively.
Resveratrol has estrogenic effect in Chinese materia medica preparation disclosed by the invention, can regulate and control gonad cell apoptosis, repairs
Hypoovarianism improves ovary maturity rate, the renal cells for mitigating diabetes renal fibrosis, alleviating high glucose induction
Apoptosis improves renal function.Endothelial cells injury induced by oxidative can be repaired simultaneously, inhibit renal tissue response to oxidative stress.Also having can
Memory is improved, the mitochondrial pathways are regulated and controled, protects cranial nerve cell.The curative effect in treatment chronic renal disease and antidotal therapy
Reliably.
Claims (4)
1. a kind of Bushen Huoxue Fang, which is characterized in that by the following Chinese medicinal composition calculated by weight: 5-15 parts of fructus lycii, tiger
4-8 parts of cane, 5-15 parts of mulberries, 5-15 parts of Radix Angelicae Sinensis, 2-6 parts of Radix Notoginseng.
2. a kind of Bushen Huoxue Fang according to claim 1, which is characterized in that by the following Chinese medicine group calculated by weight
At: 10 parts of fructus lycii, 6 parts of polygonum cuspidate, 10 parts of mulberries, 10 parts of Radix Angelicae Sinensis, 4 parts of Radix Notoginseng.
3. a kind of Bushen Huoxue Fang according to claim 1, preparation method are as follows:
(1) fructus lycii, polygonum cuspidate, mulberries, Radix Angelicae Sinensis and Radix Notoginseng are weighed by weight, and medicinal material is pre-processed；
(2) pretreated medicinal material adds the 30-60% ethyl alcohol of 4-10 times of medicinal material amount one step up, impregnates 1-21 days, medical filtration
After be centrifuged, take supernatant；
(3) the resulting supernatant of previous step is placed in evaporating dish, rotation is evaporated, pressure 30-50Pka, 30-70 DEG C of temperature, is turned
Fast 60-90 r/min；It takes out, sets and cool down 10-60 min in dry vessel to get Chinese medical concrete.
4. a kind of preparation method of Bushen Huoxue Fang according to claim 3, it is characterised in that: the preprocess method
Including medicine materical crude slice or crushing is made.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111685324A (en) * | 2020-06-05 | 2020-09-22 | 郑思念 | Composition for relaxing bowels, helping sleep and improving immunity and preparation method and application thereof |
| CN112098563A (en) * | 2020-10-14 | 2020-12-18 | 中国中医科学院望京医院(中国中医科学院骨伤科研究所) | Detection method for chemical components of kidney-tonifying and blood-activating formula |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111685324A (en) * | 2020-06-05 | 2020-09-22 | 郑思念 | Composition for relaxing bowels, helping sleep and improving immunity and preparation method and application thereof |
| CN112098563A (en) * | 2020-10-14 | 2020-12-18 | 中国中医科学院望京医院(中国中医科学院骨伤科研究所) | Detection method for chemical components of kidney-tonifying and blood-activating formula |
| CN112098563B (en) * | 2020-10-14 | 2022-07-15 | 中国中医科学院望京医院(中国中医科学院骨伤科研究所) | Detection method for chemical components of kidney-tonifying and blood-activating formula |
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