CN109223236A - Experimental animal carcass sample nervous centralis fast accurate cuts device and cuts method - Google Patents
Experimental animal carcass sample nervous centralis fast accurate cuts device and cuts method Download PDFInfo
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- CN109223236A CN109223236A CN201710562444.8A CN201710562444A CN109223236A CN 109223236 A CN109223236 A CN 109223236A CN 201710562444 A CN201710562444 A CN 201710562444A CN 109223236 A CN109223236 A CN 109223236A
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- 238000010171 animal model Methods 0.000 title claims abstract description 92
- 239000010868 animal carcass Substances 0.000 title claims abstract description 83
- 238000000034 method Methods 0.000 title claims abstract description 24
- 210000000988 bone and bone Anatomy 0.000 claims abstract description 22
- 210000004556 brain Anatomy 0.000 claims description 35
- 210000000278 spinal cord Anatomy 0.000 claims description 35
- 210000003625 skull Anatomy 0.000 claims description 15
- 239000000463 material Substances 0.000 claims description 11
- 210000000613 ear canal Anatomy 0.000 claims description 8
- 238000003698 laser cutting Methods 0.000 claims description 4
- 210000004761 scalp Anatomy 0.000 claims description 3
- 241000700159 Rattus Species 0.000 description 19
- 238000010586 diagram Methods 0.000 description 11
- 210000005036 nerve Anatomy 0.000 description 9
- 241000283973 Oryctolagus cuniculus Species 0.000 description 5
- 241000282326 Felis catus Species 0.000 description 4
- 238000006243 chemical reaction Methods 0.000 description 4
- 239000000835 fiber Substances 0.000 description 3
- 230000006870 function Effects 0.000 description 3
- 238000010297 mechanical methods and process Methods 0.000 description 3
- 210000000653 nervous system Anatomy 0.000 description 3
- 230000008520 organization Effects 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 239000008280 blood Substances 0.000 description 2
- 210000004369 blood Anatomy 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 210000004126 nerve fiber Anatomy 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 241001465754 Metazoa Species 0.000 description 1
- 230000037182 bone density Effects 0.000 description 1
- 230000019771 cognition Effects 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 210000000883 ear external Anatomy 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000001537 neural effect Effects 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 230000008807 pathological lesion Effects 0.000 description 1
- 230000035790 physiological processes and functions Effects 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61D—VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
- A61D1/00—Surgical instruments for veterinary use
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61D—VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
- A61D3/00—Appliances for supporting or fettering animals for operative purposes
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- Animal Behavior & Ethology (AREA)
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- Surgery (AREA)
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- Sampling And Sample Adjustment (AREA)
Abstract
The present invention relates to neurotomy and Bioexperiment field, discloses a kind of experimental animal carcass sample entirety nervous centralis fast accurate and cut device and cut method, it includes: chassis, front tooth fixed point, tail bone fixed point and two ear bars that this, which cuts device,；A pillar perpendicular to chassis is further fixed on chassis；The cross bar for being parallel to chassis is fixed on pillar；It is fixed with the positioning that one is parallel to cross bar on cross bar and pillar and corresponds to scale；A vertical rod perpendicular to chassis is also removably affixed on cross bar；Vertical rod can relative to above and below chassis, front and back and move left and right；The bottom end of vertical rod is fixed with cutting part and pilot pin；Pilot pin passes through mobile vertical rod, positions to the bregma or backbone of experimental animal carcass sample；Cutting part is used for after the completion of positioning, carries out the transverse cuts perpendicular to experimental animal carcass sample backbone direction to experimental animal carcass sample.It is quick, simple and laborsaving to cut operation for nervous centralis accurate positioning.
Description
Technical field
The present invention relates to neurotomy and Bioexperiment field, in particular to a kind of experimental animal (rat, mouse, cavy,
Rabbit, cat etc.) cadaver sample entirety nervous centralis fast accurate cuts device and cuts method.
Background technique
Rat and mouse are most common experimental animals, more particularly, to the research of nervous system, to the natural section of nerve
Cognition is learned nearly all from experimental animals such as rat and mouse.
Research to nervous system, many experiments are based on the morphology and electrophysiologic study to brain and spinal cord.Brain and ridge
Marrow is located at skull and intraspinal tube, since nervous centralis is that nature develops product the most advanced, the operation of nervous system
Extremely stable internal environment is needed, therefore, the canalis spinalis that the cranial cavity and vertebra that skull is constituted are constituted is very securely and close.
For primary scholar and postgraduate, studies brain and the primary difficulty of spinal cord is how quickly and accurately will
Brain or spinal cord take out from cranial cavity or intraspinal tube.The bone density of adult even aged rats, especially rat, skull and vertebra is very
Height opens skull, canalis spinalis not only needs highly difficult technology, it is also desirable to which strength on hand, especially schoolgirl small for hand-power come
It says, challenges very big.Often during opening cranium or opening canalis spinalis, it is damaged to nerve fiber.
Deputy difficulty is the positioning of brain and spinal cord.It is understood that nervous centralis is to develop to obtain nature the most accurate
Organ, space structure have strict order.That is, where being that nerve nucleus or what nerve are fine spatially
Beam is tieed up, is all determining.What core group or fibre bundle be at what position conversely speaking, be also all it is determining, this is referred to as nerve
Positioning.In general, the complete displaying of full maincenter is only done, the interest of researcher is all relatively fixed and concentrates on nervous centralis
Some position (core rolls into a ball nerve fibre bundle or structure).
And due to nerve nucleus or the nerve fibre having compares limitation or structure is smaller, how accurately to take purpose
Object, strictly a test.Sometimes materials can not get desired structure position；Sometimes it is not lost to ensure, it often again can mistake
It draws materials on a large scale, causes secondary location difficulty；Sometimes materials need to complete as early as possible, some destination proteins blood stopping after,
It can be denaturalized or be metabolized quickly, need quick fixation；It is ground sometimes for taking a certain position of nervous centralis to carry out physiological function
Study carefully, after needing quickly to draw materials, nerve fiber is immersed in artificial physiological environment (culture solution).All these operations require fast
Speed is completed, and is reduced to the pathologic lesion of nerve caused by stopping because of blood, keeps and safeguard the physiological status of nerve as far as possible.
Therefore, in order to solve the problems, such as quick and precisely taking for nervous centralis (that is: brain and spinal cord) sample, one is needed at present
Kind experimental animal (rat, mouse, cavy, rabbit, cat etc.) cadaver sample entirety nervous centralis fast accurate cuts device and cuts
Method.
Summary of the invention
The purpose of the present invention is to provide a kind of experimental animal carcass sample entirety nervous centralis fast accurate cut device and
Method is cut, it is quick, simple and laborsaving to cut operation for nervous centralis accurate positioning.
In order to solve the above technical problems, embodiments of the present invention disclose a kind of experimental animal carcass sample entirety maincenter
Neural fast accurate cuts device, comprising:
Chassis, front tooth fixed point, tail bone fixed point and two ear bars；
The front tooth fixed point, tail bone fixed point and two ear bars are separately fixed on the chassis；
The front tooth fixed point, for fixing the front tooth of the experimental animal carcass sample；
The tail bone fixed point, for fixing the first coccygeal vertebra of the experimental animal carcass sample；Tail bone fixed point is located at
It is not a changeless point on the vertical extension line at two ear bar fixed point line midpoints, it is long according to the body of experimental animal
And depending on the position of first coccygeal vertebra；
Described two ear bars are respectively used to fix the left side external auditory canal and right side external ear of the experimental animal carcass sample
Road；
A pillar perpendicular to the chassis is further fixed on the chassis；
The cross bar for being parallel to the chassis is fixed on the pillar；
The positioning that one is parallel to the cross bar, which is fixed with, on the cross bar and the pillar corresponds to scale；
It is also removably affixed a vertical rod perpendicular to the chassis on the cross bar；
The vertical rod can relative to above and below the chassis, front and back and move left and right；
The bottom end of the vertical rod is fixed with cutting part and pilot pin；
The pilot pin determines the bregma or backbone of the experimental animal carcass sample by the mobile vertical rod
Position and fixation；
The cutting part is used for after the completion of positioning, carries out the experimental animal carcass sample perpendicular to the experiment
The transverse cuts in animal carcass sample backbone direction.
Embodiments of the present invention also disclose a kind of experimental animal carcass sample entirety nervous centralis fast accurate and cut
Method cuts device suitable for experimental animal carcass sample entirety nervous centralis fast accurate described above, includes the next steps:
By front tooth fixed point, tail bone fixed point and two ear bars, the experimental animal carcass sample is fixed on described
It cuts on device chassis；
The scalp of the experimental animal carcass sample is cut, bregma, mobile vertical rod, by pilot pin perpendicular positioning are found
In the bregma of the experimental animal carcass sample, to the brain and spinal cord of the experimental animal carcass sample on positioning corresponding scale
It is positioned；
Pilot pin is vertically located in the experimental animal carcass sample according to corresponding scale is positioned by mobile vertical rod
On brain or spinal cord；
It is long according to the specific body of experimental animal carcass sample, preset " different body length brain or spinal cord position table " is inquired, really
Position the location of reading of corresponding scale；
Cutting part is started, the experimental animal carcass sample is carried out perpendicular to the experimental animal carcass sample backbone
The brain of the experimental animal carcass sample or spinal cord are cut by the transverse cuts in direction together together with skull or canalis spinalis；
The brain of the experimental animal carcass sample or spinal cord are stripped out from the skull or canalis spinalis, materials are completed.
Compared with prior art, the main distinction and its effect are embodiment of the present invention:
Four point positioning, phase are carried out to experimental animal carcass sample by front tooth fixed point, tail bone fixed point and two ear bars
Compared with traditional three-point fox method, the function to spinal cord positioning is increased, positions more accurate, conveniently, fixation is also more firm.
In addition, can save ear bar under simple and fast operation mode and fix, front tooth and first coccygeal vertebra are only fixed.
Further, during nervous centralis positioning, cutting, first having to the reason of finding bregma is, bregma
(Bregma) be nervous centralis positioning origin, the core of all brains and spinal cord rolls into a ball positioning, is all based on the origin of bregma.
Further, the scale that corresponding scale is equipped with corresponding brain or spinal cord position is positioned, it is fixed to read using positioning pointer
Scale on the corresponding scale in position, the scale of required corresponding brain or spinal cord is found according to conversion relation, carries out destination organization
Positioning positions more accurate.
Further, or mechanical method is not had to, but electronic data after pilot pin is located in bregma, is shown
It is shown as " 0 ", then running fix needle reaches " reading " position needed, is cut.
Further, the function of pilot pin can be replaced by cutting part itself, to save the setting of pilot pin.It cuts
It cuts after component directly positions and is cut, operated more simple.
Further, the transverse cuts perpendicular to backbone direction, Ke Yigeng are carried out by electric circular saw or laser cutting head
Accelerate speed, effortlessly complete materials operation.
Further, departing from whole block-like brain or spinal cord with bone, bone can be particularly simple separated from.
Further, height adjusting support foot is arranged below in chassis, can preferably adjust the operation height for cutting device, make
It uses more convenient.
Detailed description of the invention
Fig. 1 is that a kind of experimental animal carcass sample entirety nervous centralis fast accurate is cut in first embodiment of the invention
The structural schematic diagram of device；
Fig. 2 is the knot for the other side that experimental animal carcass sample entirety nervous centralis fast accurate shown in FIG. 1 cuts device
Structure schematic diagram；
Fig. 3 is that a kind of experimental animal carcass sample entirety nervous centralis fast accurate is cut in first embodiment of the invention
The use state diagram of device；
Fig. 4 is the schematic diagram of skull three-point fix in first embodiment of the invention；
Fig. 5 is the schematic diagram of four point positioning in first embodiment of the invention；
Fig. 6 is that a kind of experimental animal carcass sample entirety nervous centralis fast accurate is cut in second embodiment of the invention
The flow diagram of method.
Specific embodiment
In the following description, in order to make the reader understand this application better, many technical details are proposed.But this
The those of ordinary skill in field is appreciated that even if without these technical details and many variations based on the following respective embodiments
And modification, each claim of the application technical solution claimed can also be realized.
To make the object, technical solutions and advantages of the present invention clearer, below in conjunction with attached drawing to implementation of the invention
Mode is described in further detail.
First embodiment of the invention is related to a kind of experimental animal (rat, mouse, cavy, rabbit, cat etc.) cadaver sample
Whole nervous centralis fast accurate cuts device.
The present invention can not only can be used for rat, mouse with multi-use, but also can be used for other medium and small experimental animals, such as
Cavy, rabbit, cat etc.；Certainly, the present invention can also be made into respectively mouse is dedicated, rat is dedicated, cavy is dedicated, rabbit is dedicated,
Cat is dedicated equal a variety of.
Below by experimental animal rat, described for mouse.
Fig. 1 is the structural schematic diagram that the experimental animal carcass sample entirety nervous centralis fast accurate cuts device, and Fig. 2 is figure
Experimental animal carcass sample entirety nervous centralis fast accurate shown in 1 cuts the structural schematic diagram that device is looked over from the other side,
Fig. 3 is that the experimental animal carcass sample entirety nervous centralis fast accurate cuts the use state signal of device in actual use
Figure.
Specifically, as shown in Figure 1, Figure 2 and Figure 3, which cuts out
The device is taken to include:
Chassis 01, front tooth fixed point 02, tail bone fixed point 03 and two ear bars 12；
Front tooth fixed point 02, tail bone fixed point 03 and two ear bars 12 are separately fixed on chassis 01；
Front tooth fixed point 02, for fixing the front tooth of experimental animal carcass sample 13；
Tail bone fixed point 03, for fixing the first coccygeal vertebra of experimental animal carcass sample 13；Tail bone fixed point 03 is located at two
It is not a changeless point, according to experimental animal carcass sample on the vertical extension line at a ear bar fixed point line midpoint
Depending on body is long and the position of first coccygeal vertebra；
Two ear bars 12 are respectively used to the left side external auditory canal and right side external auditory canal of fixed experimental animal carcass sample 13；
A pillar 04 perpendicular to chassis 01 is further fixed on chassis 01；
The cross bar 05 for being parallel to chassis is fixed on pillar 04；
The positioning that one is parallel to cross bar 05, which is fixed with, on cross bar 05 and pillar 04 corresponds to scale 06；
A vertical rod 07 perpendicular to chassis 01 is also removably affixed on cross bar 05；
Vertical rod 07 relative to about 01 chassis, front and back and can move left and right；
The bottom end of vertical rod 07 is fixed with cutting part 08 and pilot pin 09；
Pilot pin 08 passes through mobile vertical rod 07, and the bregma or backbone of experimental animal carcass sample 13 are positioned and consolidated
It is fixed；
Cutting part 08 is used for after the completion of positioning, carries out experimental animal carcass sample 13 perpendicular to experimental animal carcass
The transverse cuts in sample backbone direction.
In the present embodiment, it is preferable that cutting part 08 is electric circular saw or laser cutting head.
The transverse cuts perpendicular to backbone direction are carried out by electric circular saw or laser cutting head, it can more quick, province
Complete to power materials operation.
In the present embodiment, it is preferable that
It positions corresponding scale 06 and is equipped with the brain of corresponding experimental animal carcass sample 13 or the scale of spinal cord position.
The positioning pointer 10 be directed toward and position the scale on corresponding scale 06 is additionally provided on vertical rod 07.
The scale that corresponding scale is equipped with corresponding brain or spinal cord position is positioned, is read using positioning pointer and positions corresponding scale
On scale, find the scale of required corresponding brain or spinal cord according to conversion relation, carry out destination organization positioning, positioning is more
Add accurate.
Further, it is preferable to ground, or do not have to mechanical method, but electronic data, before pilot pin is located in
After fontanel, " 0 " is shown as, and then running fix needle reaches " reading " position needed, is cut.
In the present embodiment, it is preferable that the experimental animal carcass sample entirety nervous centralis fast accurate cuts device also
Including kinematic axis and lock 11, vertical rod 07 is moved by kinematic axis, is fixed by lock.
Further, it is preferable that height adjusting support foot (not shown go out) is arranged below in chassis 01, can preferably adjust
Section cuts the operation height of device, more convenient to use.
As shown in figure 3, in actual use, rat corpses sample 13 being fixed on by four-point positioning method and cuts device
On chassis 01.
Fig. 4 shows the three-point fix schematic diagram of rat sample skull, and Fig. 5 shows the four point positioning schematic diagram of rat.
Specifically, as shown in Figure 4 and Figure 5, using the front tooth 15 of rat sample and left and right external auditory canal as strong point (front tooth one
Point, two sides external auditory canal two o'clock, 3 points), the skull of rat sample is fixed；Then the body of rat sample is straight, to protect
Card backbone is vertical with ear bar, then the first coccygeal vertebra (the 4th point) of fixed rat sample, as four-point positioning method.Pass through four point positioning
The fixation of method, the entire skull of rat sample and the anatomical location that a standard is just fixed on canalis spinalis.Subsequent materials
Exactly carry out based on this.
Four point positioning, phase are carried out to experimental animal carcass sample by front tooth fixed point, tail bone fixed point and two ear bars
Compared with traditional three-point fox method (that is: the ear bar of the three-point fix of skull, front tooth and two sides external auditory canal positions), increase to ridge
The function of marrow positioning, positions more accurate, conveniently, and fixation is also more firm.Under simple and fast operation mode, it can also save
But ear bar is fixed, only fixed front tooth and first coccygeal vertebra.
Then again by mobile vertical rod, brain and spinal cord to the experimental animal carcass sample be fixed, position and
Cutting.
Second embodiment of the invention is related to a kind of experimental animal carcass sample entirety nervous centralis fast accurate side of cutting
Method.Fig. 6 is the flow diagram that the experimental animal carcass sample entirety nervous centralis fast accurate cuts method.
Specifically, as shown in fig. 6, the experimental animal carcass sample entirety nervous centralis fast accurate cuts method includes
Following steps:
In step 601, by front tooth fixed point, tail bone fixed point and two ear bars, experimental animal carcass sample is consolidated
It is scheduled on and cuts on device chassis.
Then into step 602, the scalp of experimental animal carcass sample is cut, finds bregma, mobile vertical rod will be determined
Position needle is vertically located in the bregma of experimental animal carcass sample, on positioning corresponding scale to the brain of experimental animal carcass sample and
Spinal cord is positioned.
Bregma (Bregma, as shown in 14 in Fig. 4) is the origin of nervous centralis positioning.The core group of all brain and spinal cord
Positioning, is all based on the origin of bregma.Bregma is also apparent in aged rats, will not be disappeared.
Then into step 603, mobile vertical rod, according to corresponding scale is positioned, vertically by pilot pin (or cutting part)
It is located on the brain or spinal cord of the experimental animal carcass sample.
According to the specific body of experimental animal carcass sample it is long (rather than simple age in days because different genetic background and
Under nutritional condition, age in days does not determine the body size of animal), preset " different body length brain or spinal cord position table " is inquired, is determined
Position the location of reading of corresponding scale；
The long positioning with brain and spinal cord of the specific body of experimental animal carcass sample, there is a set of conversion mode；
The scale that corresponding scale is equipped with corresponding brain or spinal cord position is positioned, is read using positioning pointer and positions corresponding scale
On scale, find the scale of required corresponding brain or spinal cord according to conversion relation, carry out destination organization positioning, positioning is more
Add accurate.
Further, or mechanical method is not had to, but electronic data after pilot pin is located in bregma, is shown
It is shown as " 0 ", then running fix needle reaches " reading " position needed, is cut.
Then into step 604, cutting part is started, experimental animal carcass sample is carried out perpendicular to experimental animal carcass
The transverse cuts in sample backbone direction cut down the brain of experimental animal carcass sample or spinal cord together with skull or canalis spinalis together
Come.
Then into step 605, the brain of experimental animal carcass sample or spinal cord are separated from the skull or canalis spinalis
Come, materials are completed.
Departing from whole block-like brain or spinal cord with bone, it can be simply separated from bone, materials are completed, entire mistake
Journey is whole cuts.
First embodiment is device embodiments corresponding with present embodiment, and present embodiment can be implemented with first
Mode is worked in coordination implementation.The relevant technical details mentioned in first embodiment are still effective in the present embodiment, in order to
It reduces and repeats, which is not described herein again.Correspondingly, the relevant technical details mentioned in present embodiment are also applicable in the first implementation
In mode.
It should be noted that in the claim and specification of this patent, such as first and second or the like relationship
Term is only used to distinguish one entity or operation from another entity or operation, without necessarily requiring or implying
There are any actual relationship or orders between these entities or operation.Moreover, the terms "include", "comprise" or its
Any other variant is intended to non-exclusive inclusion so that include the process, methods of a series of elements, article or
Equipment not only includes those elements, but also including other elements that are not explicitly listed, or further include for this process,
Method, article or the intrinsic element of equipment.In the absence of more restrictions, being wanted by what sentence " including one " limited
Element, it is not excluded that there is also other identical elements in the process, method, article or apparatus that includes the element.
Although being shown and described to the present invention by referring to some of the preferred embodiment of the invention,
It will be understood by those skilled in the art that can to it, various changes can be made in the form and details, without departing from this hair
Bright spirit and scope.
Claims (7)
1. a kind of experimental animal carcass sample entirety nervous centralis fast accurate cuts device characterized by comprising
Chassis, front tooth fixed point, tail bone fixed point and two ear bars；
The front tooth fixed point, tail bone fixed point and two ear bars are separately fixed on the chassis；
The front tooth fixed point, for fixing the front tooth of the experimental animal carcass sample；
The tail bone fixed point, for fixing the first coccygeal vertebra of the experimental animal carcass sample；The tail bone fixed point is located at
It is not a changeless point on the vertical extension line at two ear bar fixed point line midpoints, it is long according to the body of experimental animal
And depending on the position of first coccygeal vertebra；
Described two ear bars are respectively used to fix the left side external auditory canal and right side external auditory canal of the experimental animal carcass sample；
A pillar perpendicular to the chassis is further fixed on the chassis；
The cross bar for being parallel to the chassis is fixed on the pillar；
The positioning that one is parallel to the cross bar, which is fixed with, on the cross bar and the pillar corresponds to scale；
It is also removably affixed a vertical rod perpendicular to the chassis on the cross bar；
The vertical rod can relative to above and below the chassis, front and back and move left and right；
The bottom end of the vertical rod is fixed with cutting part and pilot pin；
The pilot pin positions the bregma or backbone of the experimental animal carcass sample by the mobile vertical rod；
The cutting part is used for after the completion of positioning, carries out the experimental animal carcass sample perpendicular to the experimental animal
The transverse cuts in cadaver sample backbone direction.
2. experimental animal carcass sample entirety nervous centralis fast accurate according to claim 1 cuts device, feature exists
In the cutting part is electric circular saw or laser cutting head.
3. experimental animal carcass sample entirety nervous centralis fast accurate according to claim 1 cuts device, feature exists
In the scale for positioning corresponding scale and being equipped with the brain or spinal cord position of the corresponding experimental animal carcass sample.
4. experimental animal carcass sample entirety nervous centralis fast accurate according to claim 3 cuts device, feature exists
In being additionally provided on the vertical rod and be directed toward the positioning pointer for positioning the scale on corresponding scale.
5. experimental animal carcass sample entirety nervous centralis fast accurate according to claim 1 cuts device, feature exists
In, further include kinematic axis and lock, the vertical rod is moved by the kinematic axis, by it is described lock be fixed.
6. experimental animal carcass sample entirety nervous centralis fast accurate according to claim 1 cuts device, feature exists
In height adjusting support foot is arranged below in the chassis.
7. a kind of experimental animal carcass sample entirety nervous centralis fast accurate cuts method, appoint suitable for claim 1 to 6
Experimental animal carcass sample entirety nervous centralis fast accurate described in one cuts device, which comprises the following steps:
By front tooth fixed point, tail bone fixed point and two ear bars, the experimental animal carcass sample is fixed on described cut
On device chassis；
The scalp of the experimental animal carcass sample is cut, bregma is found, pilot pin is vertically located in institute by mobile vertical rod
The bregma for stating experimental animal carcass sample carries out the brain and spinal cord of the experimental animal carcass sample on positioning corresponding scale
Positioning；
Mobile vertical rod, according to positioning corresponding scale, by pilot pin be vertically located in the experimental animal carcass sample brain or
On spinal cord；
It is long according to the specific body of experimental animal carcass sample, preset " different body length brain or spinal cord position table " is inquired, it is fixed to determine
The location of reading of the corresponding scale in position；
Cutting part is started, the experimental animal carcass sample is carried out perpendicular to the experimental animal carcass sample backbone direction
Transverse cuts, the brain of the experimental animal carcass sample or spinal cord are cut together together with skull or canalis spinalis；
The brain of the experimental animal carcass sample or spinal cord are stripped out from the skull or canalis spinalis, materials are completed.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU198263U1 (en) * | 2020-04-20 | 2020-06-29 | Федеральное государственное бюджетное учреждение «Национальный медицинский исследовательский центр радиологии» Министерства здравоохранения Российской Федерации (ФГБУ «НМИЦ радиологии» Минздрава России) | DEVICE FOR FIXING LABORATORY ANIMALS WITH LOCAL RADIATION OF THE BRAIN |
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