CN109221160A - A kind of bactericidal composition - Google Patents
A kind of bactericidal composition Download PDFInfo
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- CN109221160A CN109221160A CN201710556224.4A CN201710556224A CN109221160A CN 109221160 A CN109221160 A CN 109221160A CN 201710556224 A CN201710556224 A CN 201710556224A CN 109221160 A CN109221160 A CN 109221160A
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- plant
- bactericidal composition
- amisulbrom
- fenamidone
- bacterium
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/64—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with three nitrogen atoms as the only ring hetero atoms
- A01N43/647—Triazoles; Hydrogenated triazoles
- A01N43/653—1,2,4-Triazoles; Hydrogenated 1,2,4-triazoles
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- Agronomy & Crop Science (AREA)
- Pest Control & Pesticides (AREA)
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- Health & Medical Sciences (AREA)
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Abstract
The present invention provides a kind of bactericidal composition, and the bactericidal composition active constituent contains amisulbrom and epoxiconazole, and wherein the weight percent of amisulbrom and epoxiconazole is 25:1-1:25.The invention further relates to the bactericidal compositions for controlling downy mildew, late blight, early blight, leaf spot, banded sclerotial blight, gray mold, powdery mildew, damping-off, soft rot, leaf blight, anthracnose, green mould, shot hole, spot defoliation, bacterialo wilt disease, black spot, white beautiful disease, samping off, leaf mold, spot defoliation, brown spot, false smut, rice blast, head blight, the purposes of rust.The purposes of pathogenic or saprophytic fungi and bacterium in the place prevention and treatment soil or cultivation medium prevented and treated needed for being applied to the invention further relates to the bactericidal composition.It is used to handle seed the invention further relates to the bactericidal composition with the purposes for the phytopathogen invasion for protecting seed to carry.
Description
Technical field
The present invention relates to a kind of bactericidal compositions；The invention further relates to use the bactericidal composition to prevent and treat harmful fungoid
Method.
Background technique
At present for being agriculturally also easy to produce the disease control of resistance, the pesticide species for using the mechanism of action different carry out mixture
It is optimal selection, if proportion is rationally, can produce significant synergistic effect, field control effect is made to be substantially better than each list
The effect of agent.Fungicide containing single active ingredient is usually present certain defect, continuous several times in agricultural disease prevention and treatment
Using not only making pathogen be also easy to produce drug resistance, and easily cause the pollution to food and environment, by fungicidal activity at point it
Between rational mixture can overcome disadvantage mentioned above.The synergistic effect that reasonable compounding generates effective component, can be improved preventive effect, subtracts
Few effective ingredient dosage, save the cost delays the generation of the drug resistance of pathogen, and then can reduce or even avoid pesticide to food
The pollution of object and environment.
About pesticide activity, especially to crop protection, the key problem for the research carried out in the technical field first is that
Improve the performance in terms of performance, especially bioactivity and the performance in terms of keeping this activity within a certain period of time.
Amisulbrom (Amisulbrom), entitled 3- (the fluoro- 2 methyl indole -1- sulfonyl of the bromo- 6- of the 3-)-N of chemistry,
N- dimethyl -1,2,4- triazole -1- sulfonamide, chemical structural formula are as follows:
Amisulbrom compound is the sulfamoyl compounds of Japanese Nissan chemical industry Co., Ltd. exploitation, is existed
It is disclosed in CN1158278C.Amisulbrom has very high bactericidal activity to epidemic disease and downy mildew.
Amisulbrom, there is no adequately effect is played, it is weak or hold to show therapeutic effect to certain plants harmful fungoid
The effect phase is relatively short, so that in some applications, amisulbrom shows actually insufficient control action.
Due to now constantly improve to the environmental requirement of fungicide and economic requirement, such as to activity profile, toxicity, selection
Property, rate of application, residue composition and it is advantageous preparation feasibility requirement, additionally due to that may be deposited in terms of such as drug resistance
In problem, therefore, exploitation is in some aspects lasting task better than the new fungicide of existing fungicide.
Summary of the invention
There can be greater activity the present invention provides one kind and activity keeps more long bactericidal composition.Of the invention
Bactericidal composition plays synergy, is suitable for prevention and treatment harmful fungoid.
A kind of long-term reactive compound of single administration, which carrys out controlling disease, will lead to disease drug resistance generation, cause compound anti-
Effect decline, or even thoroughly lose preventive effect.In order to reduce the risk that phytopathogen develops drug resistance, prevented and treated using synergistic composition
Noxious plant disease fungus be at present frequently with one of method.
The present invention provides a kind of bactericidal composition, the composition is by carrying out group for amisulbrom and Fenamidone
It closes, so that obtained composition has gain effect in control efficiency, and has expanded fungicidal spectrum, play what a medicine was mostly used
Effect, effectively slows down or germ is avoided to develop drug resistance.Astoundingly, the bactericidal activity of bactericidal composition of the invention is than each
The active adduction of a reactive compound is considerably higher.In other words, there are unpredictable, necessary being synergistic effects, and
Not only active supplement.
We have found that simultaneously, i.e., jointly or separately applying amisulbrom and Fenamidone, or successively apply indazole sulphur
Bacterium amine and Fenamidone preferably to prevent and treat harmful fungoid than each compound is administered alone.
Amisulbrom and Fenamidone are combined, just reduce application in the case where reduced reactive compound applies total amount
For the activity profile of rate and improvement known compound amisulbrom and Fenamidone, the present invention is reducing reactive compound application
Under total amount, having to harmful fungoid improves active (synergy).
When reactive compound is present in bactericidal composition of the invention with specific weight ratio, synergistic effect is especially bright
It is aobvious.But the weight ratio of the reactive compound in bactericidal composition of the present invention can change in a certain range.
A kind of bactericidal composition of the present invention is to adopt the following technical solutions to achieve:
A kind of bactericidal composition, it is characterised in that: the bactericidal composition contains active constituent amisulbrom and Fenamidone,
Wherein the weight percent of amisulbrom and Fenamidone be 50:1-1:50,25:1-1:25, further preferably 20:1-1:
20, it is further preferably 15:1-1:15, more preferably 10:1-1:10, more preferably 5:1-1:5.
In the present invention, the weight percent of amisulbrom and Fenamidone can also be 25:1,24:1,23:1,22:1,
21:1,20:1,19:1,18:1,17:1,16:1.,15:1,14:1,13:1,12:1,11:1,10:1,9:1,8:1,7:1,6:1,
5:1,4:1,3:1,2:1,1:1,1:2,1:3,1:4,1:5,1:6,1:7,1:8,1:9,1:10,1:11,1:12,1:13,1:14,
1:15,1:16,1:17,1:18,1:19,1:20,1:21,1:22,1:23,1:24,1:25。
A kind of bactericidal composition also includes filler and/or surface containing active constituent amisulbrom and Fenamidone
Activating agent.
The bactericidal composition dosage form be dispersible oil-suspending agent, suspending agent, seed coat agent, suspoemulsion, wettable powder,
Water dispersible granules, coated granule, squeeze out granule, missible oil, microemulsion, aqueous emulsion, effervescent tablet, super-low capacity at microcapsule suspending agent
Measure liquor, dry suspension, electrostatic oil agent.
The bactericidal composition, wherein the content of the active constituent of amisulbrom and Fenamidone accounts for composition
5%-90%, preferably 10%-80%, more preferable 20%-70%.
In bactericidal composition of the invention, the quality of amisulbrom and Fenamidone accounts for the bactericidal composition with weight
Meter can also be, for example:
5%,6%,7%,8%,9%,10%,11%,12%,13%,14%,15%,16%,17%,18%,19%,20%,21%,22%,23%,
24%,25%,26%,27%,28%,29%,30%,31%,32%,33%,34%,35%,36%,37%,38%,39%,40%,41%,42%,
43%,44%,45%,46%,47%,48%,49%,50%,51%,52%,53%,54%,55%,56%,57%,58%,59%,60%,61%,
62%,63%,64%,65%,66%,67%,68%,69%,70%,71%,72%,73%,74%,75%,76%,77%,78%,79%,80%,
81%,82%,83%,84%,85%,86%,87%,88%,89%,90%。
A method of prevention and treatment plant pathogen, by bactericidal composition of the invention act on plant pathogen and/or its
Environment or plant, plant part, plant propagation material and the plant organ then grown, soil or cultivation medium, material or
In space.
A method of bactericidal composition of the present invention is acted on plant or plant portion by prevention and treatment plant pathogen
Point.
A method of prevention and treatment plant pathogen, by bactericidal composition of the present invention act on plant propagation material and
The plant organ then grown.
A method of bactericidal composition of the present invention is acted on soil or cultivation matchmaker by prevention and treatment plant pathogen
It is situated between.
A method of prevention and treatment plant pathogen, amisulbrom and Fenamidone are administered simultaneously or respectively application or
Sequential application.
A method of prevention and treatment plant pathogen, can plant by before infecting or after infecting by the Bactericidal composite
Object acts on plant pathogen and/or its environment or plant, plant part, plant propagation material and the plant then grown
In organ, soil or cultivation medium, material or space.
A method of prevention and treatment plant pathogen, including by bactericidal composition of the invention with agronomy effectively and substantially without plant
The amount of application of object toxicity applied with seed treatment, foliage applying, stem, is impregnated with, instiled, is poured, sprayed, is sprayed, dusting, distribution or
The methods of smoke is applied to plant pathogen and/or its environment or plant, plant part, plant propagation material and subsequent length
In plant organ out, soil or cultivation medium, material or space.
The bactericidal composition is used to prevent and treat Cereal, veterinary antibiotics, harmful fungoid on ornamental plant and grapevine
Purposes.
The bactericidal composition for controlling Cereal, veterinary antibiotics, Rhizoctonia on ornamental plant and grapevine,
Verticillium Nees, knee Pseudomonas, rotten mold genus, downy mildew Pseudomonas, phytophthora, Fusarium, grey mold Pseudomonas, Erysiphe, hair
The purposes of mycosphaerella, Exserohilum category, Colletotrichum harmful fungoid.
The bactericidal composition is for controlling downy mildew, late blight, early blight, leaf spot, banded sclerotial blight, gray mold, white
Powder disease, damping-off, soft rot, leaf blight, anthracnose, green mould, shot hole, spot defoliation, bacterialo wilt disease, blackspot
Sick, white beautiful disease, samping off, leaf mold, spot defoliation, brown spot, false smut, rice blast, head blight, the purposes of rust.
The bactericidal composition is used to protect the purposes of plant, plant propagation material and the plant organ then grown.
It causes a disease in the place prevention and treatment soil or cultivation medium that the bactericidal composition is prevented and treated needed for being applied to or saprophytic
The purposes of harmful fungoid.
The bactericidal composition is used to handle seed with the purposes for the phytopathogen invasion for protecting seed to carry.
The bactericidal composition is used to protect the purposes of stock.
The bactericidal composition is for protecting stock in storage period from fungi or the purposes of bacterial invasion.
Bactericidal composition of the invention has very strong activity to various phytopathogens, and can draw to by phytopathogen
The prevention and treatment of the plant disease risen play very strong preventive effect.
Bactericidal composition of the invention plants the wide scopes such as such as Basidiomycetes, Ascomycetes, Oomycete and deuteromycetes
Object pathogenic epiphyte has excellent activity.
Oomycete, including Phytophthora (Phytophthora), such as phytophthora infestans
(Phytophthorainfestans), phytophthora sojae kaufmann&gerdemann (Phytophthoramegasperma), foot rot of citrus bacterium
(Phytophthoraparasitica), camphor tree phytophthora (Phytophthoracinnamomi) and phytophthora capsici
(Phytophthoracapsici) disease；Careless rotten mould withered category (Pythium) such as level ground grass Pythium aphanidermatu
(Pythiumaphanidermatum) disease；And Peronosporaceae (Peronosporaceae) disease such as Plasmopara viticola
(Plasmoparaviticola), peronospora (Peronospora) (including Peronosporatabacina
(Peronosporatabacina) and Peronospora parasitic bacterium (Peronosporaparasitica)), Pseudoperonospora
(Pseudoperonospora category) germ (including bacterium of downy mildew of cucumber (Pseudoperonosporacubensis) and disk stalk it is mould
Bacterium germ (Bremialactucae), pythium (Pythium) such as scraping and printing unit (Pythiumaphanidermatum);
Ascomycetes, including Alternaria (Alternaria) disease such as tomato early blight bacterium (Alternariasolani) and
Black spot of cabbage bacterium (Alternariabrassicae), ball seat Pseudomonas (Guignardia) disease such as black rot of grape bacterium
(Guignardiabidwelli), Venturia (Venturia) disease such as apple black star bacteria
(Venturiainaequalis), Septoria (Septoria) disease such as glume blight bacterium (Septorianodorum) and leaf
Blight bacterium (Septoriatritici), powdery mildew such as Erysiphe (Erysiphe) (including wheat powdery mildew
(Erysiphegraminis) and trailing plants Powdery Mildew (Erysiphepolygoni)), grape powdery mildew
(Uncinulanecatur), cucumber powdery mildew's pathogen (Sphaerothecafuligena) and apple mildew bacterium
(Podosphaeraleucotricha), wheat Phyllostachys pubescens (Pseudocercosporellaherpotrichoides) object
Kind, grey mold Pseudomonas (Botrytis) species disease such as Botrytis cinerea germ (Botrytiscinerea), Monilinia fructicola
(Moniliniafructicola) disease, sclerotium Pseudomonas (Sclerotinia) species disease such as Sclerotinia sclerotiorum
(Sclerotiniasclerotiorum), Pyricularia oryzae (Magnaporthegrisea), grape branch-rot bacterium
(Phomopsisviticola) disease, compacted shape Pseudomonas (Helminthosporium) species disease such as Exserohilum turcicum
(Helminthosporiumtriticirepentis), reticulate pattern germ (Pyrenophorateres) species, anthrax disease is for example
Black fruit bacterium (Glomerella) or colletotrichum (Colletotrichum category) disease (such as fine strain of millet anthrax bacteria
(Colletotrichumgraminicola) and watermelon anthrax bacteria (Colletotrichumorbiculare)) and wheat it is complete
It loses germ (Gaeumannomycesgraminis);
Basidiomycetes, including the rest fungus disease as caused by Rust (Puccinia category) (such as Puccinia recondita
(Pucciniarecondita), strip rust bacteria (Pucciniastriiformis), leaf rust (Pucciniahordei), puccinia graminis bacterium
(Pucciniagraminis) and handle rest fungus (Pucciniaarachidis)), coffee rest fungus (Hemileiavastatrix) and
Phakopsora pachyrhizi sydow (Phakopsorapachyrhizi);
Deuteromycetes, including Rhizoctonia (Rhizoctonia category) species (such as Rhizoctonia solani Kuhn
(Rhizoctoniasolani) and redness of the skin or complexion hyphal cluster germ (Rhizoctoniaoryzae));Fusarium (Fusarium) disease,
Such as F.graminearum schw (Fusariumgraminearum), beads Fusariumsp (Fusariummoniliforme), fusarium oxysporum
(Fusariumoxysporum, fusarium moniliforme (Fusariumproliferatum), fusariun solani
(Fusariumsolani);Verticillium dahliae (Verticilliumdahliae);Sclerotiumrolfsii (Sclerotiumrolfsii)；Cloud
Line bacterium (Rynchosporiumsecalis);Black puckery germ (Cercosporidiumpersonatum), alternaria
(Cercosporaarachidicola) and brown patch germ (Cercosporabeticola);Silver dollar pinta bacterium
(Rutstroemiafloccosum)。
Silk core on the particularly suitable control Cereal of bactericidal composition of the invention, veterinary antibiotics, ornamental plant and grapevine
Pseudomonas, Verticillium Nees, knee Pseudomonas, rotten mold genus, downy mildew Pseudomonas, phytophthora, Fusarium, grey mold Pseudomonas, powdery mildew
The purposes of category, hair mycosphaerella, Exserohilum category harmful fungoid.
Bactericidal composition of the invention is particularly suitable for controlling downy mildew, late blight, early blight, leaf spot, banded sclerotial blight, grey mold
Disease, powdery mildew, damping-off, soft rot, leaf blight, anthracnose, green mould, shot hole, spot defoliation, bacterialo wilt disease,
Black spot, white beautiful disease, samping off, leaf mold, spot defoliation, brown spot, false smut, rice blast, head blight, the use of rust
On the way.
The suitable crop plants of bactericidal composition of the invention specifically include that cereal crops, for example, wheat, barley, oat,
Naked barley, triticale, rice, corn, sorghum and millet；Vine crop, such as Table Grape and vinifera；Field crop, example
Such as rape (Canola), sunflower；Sugar beet, sugarcane, soybean, peanut (peanut), tobacco, clover, clover, Hu Zhi
Son, clover and vetch；Pip fruit, such as apple, pears, crabapple, loquat, hawthorn and quince；Stone fruit, example
Such as peach, cherry, plum, apricot, honey peach；Citrus fruit, such as lemon, bitter orange, orange, shaddock, mandarin orange (orange) and kumquat；
Radicant and field crop (and their leaf), such as arithoke, common beet and sugar beet, carrot, cassava, life
Ginger, ginseng, horseradish, parsnip, potato, little radish, turnip, sweet potato, turnip and Chinese yam；Bulb bearing plant, such as
Garlic, leek, onion and verdant；Leaf vegetables plant, for example, mustard seed Chinese Ixeris (rocket salad), celery, celery, Chinese celery, witloof (thatch dish),
Fennel, bile ingredients and leaf lettuce, parsley, red witloof (red witloof), rheum officinale, spinach and Swiss chard；Btassica (Koryo
Dish) leaf vegetables, for example (,) it is broccoli, cauliflower (broccoli), Brussels sprouts, cabbage, Chinese cabbage, cauliflower, wild cabbage, collard, big
Head dish, leaf mustard and green vegetables；Bean (succulence or without juice) such as lupin, Kidney bean) (including semen viciae fabae, kidney bean, dish
Beans, Hua Dou, scarlet runner bean, snap beans, wide leaf vegetables beans and wax bean), Kidney bean (including red bean, asparagus bean, cow gram, crow
Cowpea, long bean, cowpea, mung bean, cowpea, urd bean and speciality cowpea), semen viciae fabae, chick-pea, melon ear, sword bean, Lens culinaris and pea
(including kidney bean, edible podded pea, field pea, pea, green pea, snow bean, sweet tea beans, pigeonpea and soybean)；Fruit vegetables, such as eggplant
Son, ground-cheery, Solanum muicatum and capsicum (including bell pepper, capsicum, capsicum for cooking, pimiento, pimento；Small tomato and tomato)；Calabash
Reed class vegetables, such as chocho (fruit), wax gourd, citron watermelon, cucumber, young cucumber, marrow squash (including cucurbit, wooden dipper
Melon), sponge gourd, gumbo, balsam apple, dioecism momordica root, balsam pear and Chinese cucumber, muskmelon, cucurbita pepo and winter squash and watermelon；Berries, it is all
Such as blackberry, blueberry, the red fruit certain kind of berries, the dew certain kind of berries, the purplish blue certain kind of berries, blueberry, Cranberry, currant, the wild certain kind of berries, Loganberry, raspberry and strawberry；Raw nut is set,
Such as almond, beech nut, Brazil nut, butternut, cashew nut, Chinese chestnut, fibert (filbert), hickory nut, Queensland nut, Mei Zhoushan
Walnut and English walnut；Tropical fruit (tree) and other crops, for example, banana, plantain, mango, coconut, pawpaw, avocado, lichee, American aloe,
Coffee, cocoa, sugarcane, oil palm, sesame, rubber and fragrance；Fibre crops, such as cotton, flax and hemp；Sod grass (including
Season type and shitivi type sod grass).
The particularly suitable crop plants of bactericidal composition of the invention include rice, cucumber, muskmelon, cabbage, grape, red
Green pepper, green pepper, watermelon, pumpkin, tobacco, citrus, apple, tomato, banana, corn, asparagus, hare's-lettuce, common calla, cauliflower, the volume heart
Dish, konjaku, rape, celery, japanese radish, tobacco, onion, green stem vegetable, tomato, eggplant, carrot, green onion, Chinese cabbage, balling
Wild cabbage, potato, romaine lettuce, oilseed rape.
Bactericidal composition of the invention is particularly suitable for controlling rice plants wilt disease, and rice plants banded sclerotial blight, rice stands withered
Disease, cucumber anthracnose, powdery mildew of cucumber, cucumber, muskmelon, cabbage, Chinese cabbage, onion and grape downy mildew, it is potato, red
Green pepper, green pepper, watermelon, pumpkin, tobacco and tomato late blight, early blight of tomato, the black spot of cedra fruits, cedra fruits
Common green mould, pear scab, alternaria leaf spot of apple, various types of gray molds, stem rot and rust, the soft corruption of Chinese cabbage
Disease, the leaf blight of corn, sigatoka, cucumber samping off, wheat leaf rust, wheat powdery mildew.
Bactericidal composition of the invention is particularly suitable for controlling following disease:
| Host plant | Disease title | Genic organisms |
| Potato | The late blight of potato | Phytophthora infestans |
| Cucumber | Cucumber downy mildew | Cuba artificial downy mildew |
| Banana | Sigatoka | Sigatoka leaf spot disease |
| Wheat | Wheat powdery mildew | Grass family dlumeria graminis |
| Wheat | Wheat leaf rust | Puccinia recondita f. sp. tritici |
| Rice | Rice seedling damping-off | Fusarium graminearum |
| Cucumber | Powdery mildew of cucumber | Melon monofilament shell powdery mildew |
| Tomato | Graw mold of tomato | The pathogen of Botrytis cinerea |
| Chinese cabbage | Soft rot of cabbage | Carrot soft rot Erwinia |
| Corn | The leaf blight of corn | Big spot Exserohilum |
Bactericidal composition of the invention can be used as foliage fungicide in crop protection, can also be used as fungicide for dress seed with
As soil fungicides.
Bactericidal composition of the invention can be used for protecting plant, plant part, plant propagation material and the plant then grown
Sundries official.
Bactericidal composition of the invention is used to protect the use of plant, plant propagation material and the plant organ then grown
On the way.
Bactericidal composition of the invention is used to handle seed with the use for the plant pathogenic fungi invasion for protecting seed to carry
On the way.
Bactericidal composition of the invention can handle all plants and plant part." plant " refers to all plants and plant
Population, such as ideal and undesirable wild plant, cultivated plant and plant variety are (regardless of whether by plant variety or plant
Cultivate the protection of human rights benefit).Cultivated plant and plant variety can be the plant obtained by Sterile culture and breeding method, this
A little methods can be aided with or be supplemented with one or more biological technique methods, such as use dihaploid, protoplast fusion, random
With orthomutation, molecule or genetic marker, or use bioengineering and genetic engineering method.Plant part refers to all of plant
Part and organ above and below the ground, such as bud, leaf, Hua Hegen, such as leaf, needle, stem, branch, flower, fructification, fruit and kind
Son and root, bulb and rhizome.It crop and nourishes and generates and case of propagation material, such as transplants, bulb, rhizome, running roots
Plant part is also belonged to seed.
A method of the bactericidal composition is acted on plant propagation material and then length by prevention and treatment plant pathogen
Plant organ out.
Term " plant propagation material " is interpreted as referring to all plant parts for having fertility, such as seed, can use
In breeding the latter and vegetable matter such as cuttage item or stem tuber (such as potato).Therefore, plant used herein
Part includes plant propagation material.It is mentioned that such as seed, root, fruit, stem tuber, bulb, rhizome and plant part.To
The germination plant and effective plant inhibited after germinateing in soil or after emergence.Young plant can be before transplantation by being impregnated into
Row whole or Local treatment are protected.
Currently preferred plant propagation material is seed.Bactericidal composition of the invention is also particularly suitable for processing seed.
Crop damage caused by most harmful fungoid is due to during storage or after sowing and in plant germination process
Or caused by the infringement of the seed after germination.Since the root and branch of growth period plant are especially sensitive and even if small damage
It can result in the death of plant.Another aspect of the present invention provides a kind of method for protecting seed and germinating plants, and this method makes
Crop protection agents are additionally being applied without additional application crop protection agents or at least significant ground after planting or after plant germination.It is another
Aspect, using bactericidal composition of the invention optimize used in reactive compound amount, with farthest provide seed and
The protection of germinating plants is against the invasion of plant pathogenic fungi, and plant itself not will receive used reactive compound
Damage.
Therefore, the present invention is also in particular to by handling seed with bactericidal composition of the invention to protect seed and hair
The method that bud plant invades from plant pathogenic fungi.The invention further relates to bactericidal compositions according to the present invention in processing kind
Son is to protect seed and germinating plants from the purposes of plant pathogenic fungi.
Harm budding after plant plant pathogenic fungi control mainly by using crop protection agents processing soil and
The aerial part of plant carries out.Healthy issuable influence in view of crop protection agents on environment and humans and animals,
It is therefore desirable to reduce the amount of application of reactive compound to the greatest extent.
Bactericidal composition according to the present invention is suitable for protection in agricultural, in greenhouse, in forestry or gardening-or vinegrowing
The seed of any plant variety of kind application.Particularly, the seed form used is cereal (such as wheat, barley, rye, black
Wheat, millet, oat), soybean, sorghum, pea, lens, corn, cotton, soybean, rice, potato, sunflower, Kidney bean, coffee
Coffee, beet, peanut, rape, olive, cocoa, sugarcane, tobacco, vegetables (such as tomato, cucumber, onion and lettuce), turfgrass and
Decoration uses plant.The processing of the seed of cereal and greengrocery is vital.
Active constituent amisulbrom and Fenamidone in bactericidal composition of the invention is individually or with suitable preparation
Form is applied to seed.It is preferably handled in the state of substantially stabilized so that processing will not cause any damage to seed
Evil.In general, can any point-in-time between picking and sowing carry out processing seed.Commonly used seed is separated from plant
And it is isolated from cob, shell, stem, epidermis, hair or pulp.Therefore, it is possible to use for example, extremely by picking, cleaning and drying
Water content is lower than 15% seed.Alternatively, it is also possible to for example with water process after using drying, the kind then dried once more
Son.
The medicament of the method for seed treatment, such as can there are, diluent liquid or solid-like does not have to dilution directly general
Seed is immersed in liquid condition solution the method for making medicament be impregnated with seed, is blended in solid chemicals or liquid preparation and seed
Together, being coated processing makes the method for the surface of the seed attachment medicament, the methods of sprinkling near seed while plantation.
Plant part and the plant organ then grown are any of the plant generated by plant propagation material such as seed
Part.Plant part, plant organ and plant also can benefit from by the way that bactericidal composition is applied to plant propagation material institute
The pathogenic damage of acquisition is protected.The plant organ grown behind certain plants part and certain places can also regard plant propagation as
Material, its own can apply (or processing) with bactericidal composition；To by processed plant part and processed plant
Plant, other plant parts and the other plant organs that organ generates also can benefit from by applying bactericidal composition
With.
It causes a disease in the place prevention and treatment soil or cultivation medium that bactericidal composition of the invention is prevented and treated needed for being applied to or saprophytic
Fungi and bacterium purposes.
A method of the bactericidal composition is acted on soil or cultivation medium by prevention and treatment plant pathogen.
Bactericidal composition of the invention can also be used to prevent or control a variety of pathogenic or saprophytic in soil or cultivation medium
Fungi and bacterium.The example of the aquacultural fungal pathogen of soil-borne includes Alternaria (Alternaria spp.), Ascochyta
(Ascochyta spp.), Botrytis cinerea (Botrytis cinerea, Cercospora (Cercospora spp., ergot
(Clavicepspurpurea), standing grain cochliobolus (Cochliobolussativus), Colletotrichum (colletotrichum
Spp., Epicoccum (Epicoccum spp., F.graminearum schw (Fusariumgraminearum), beads Fusariumsp
(Fusariummoniliforme), fusarium oxysporum (Fusariumoxysporum, fusarium moniliforme
(Fusariumproliferatum), fusariun solani (Fusariumsolani) ties up glue chain spore
(Fusariumsubglitinans), Helminthosporium (Helminthosporiumspp) avenges rotten microtorr bacterium
(Microdochiumnivale), Penicillium (Pencilliumspp), Phoma (Phoma spp.), wheat nuclear cavity bacteria
(Pyrenophoragraminea), rice blast Pyricularia Sacc. (Pyriculariaoryzae), Rhizoctonia solani Kuhn
(Rhizoctoniasolani), Rhizoctonia cerealis (Rhizoctoniacerealis), Sclerotinia (Sclerotinia
Spp.), Septoria (Septoria spp.), silk axis smut (Sphacelothecareilliana), Tilletia
(Tilletia spp.), meat spore core coral bacterium (Typhula incarnate), hidden smut (Urocystisocculta) are black
Powder Pseudomonas (Ustilago spp.), Verticillium (Verticillium spp.), Phytophthora (Phytophthora), grass are rotten mould
It is withered to belong to (Pythium), Peronospora (Peronospora), Pseudoperonospora (Pseudoperonospora).
Bactericidal composition of the invention is suitable for controlling the phytopathogen such as fusarium, rhizoctonia for leading to soil disease
Category, Verticillium Nees and knee Pseudomonas.
Under normal circumstances, soil germ can generate a large amount of thallus, as long as condition develops favorably and host pathogen growth
Be again it is susceptible, germ mass propagation and can infect host, and in the presence of susceptible host, these germs can enter
The lasting pathogenic phase, with the continuous cropping of crop mass propagation spread, but later nutrient be depleted or edaphic condition such as temperature,
When humidity etc. is unfavorable to germ, germ can enter dormant period again.In the absence of susceptible host, soil-borne disease bacterium is in the soil
It can survive, in addition to soil germ has extensive host range, moreover it is possible to be deposited on the root surface of non-host or the broken branches and fallen leaves
It is living, it is undivided with it with saprophytic competitiveness.But different germs be it is discrepant, as sickle-like bacteria almost may be used in the soil
It is survived with indefinite duration.
Cultivation medium of the present invention is the supporter for referring to make crops take root, grow, such as: soil, water etc.,
Specific raw material can be used for example sand, float stone, vermiculite, diatomite, agar-agar, gelling material, polymer substance, asbestos,
Sawdust, bark etc..
The method that medicament is applied into soil, such as liquid preparation is diluted in water or is not diluted and is directly applied to plant
The methods of in the root of body or the rice seedling bed of seedling, granule is disseminated to the side in the root of plant or the rice seedling bed of seedling
Method has the method that pulvis, water dispersible granules etc. are sprayed in soil prior to seeding and are integrally mixed with soil, before sowing or plants
Implantation hole will be sprayed on before kind of plant after pulvis, water-dispersible grain dilution agent, in seed furrow, carrying out method of sowing etc..
Bactericidal composition of the invention apply also for prevent or control harvest after and storage disease.According to the present invention, it harvests
Afterwards can be for example by caused by following fungi with the disease of storage period: Colletotrichum kind, such as banana pierce disk spore
(Colletotrichum musae), Colletotrichum gloeosporiodes (Colletotrichum gloeosporioides), capsicum thorn
Disk spore (Colletotrichum coccodes)；Fusarium kind, such as fusarium semitectum (Fusarium semitectum),
Fusarium moniliforme (Fusarium moniliforme), Fusarium solani (Fusarium solani), Fusarium oxysporum
(Fusarium oxysporum)；Verticillium dahliae belongs to kind, such as cocoa verticillium sp (Verticillium theobromae)；It is black
The mould category kind of spore；Botrytis kind, such as the pathogen of Botrytis cinerea (Botrytis cinerea)；Geotrichum, such as geotrichum candidum
(Geotrichum candidum)；Phomopsis kind, Natal Phomopsis (Phomopsis natalensis)；Color two
Spore belongs to kind, such as two spore of citrus color (Diplodia citri)；Alternaria kind, such as Altemaria citri (Alternaria
Citri), mutually every Alternariaspp (Alternaria alternata)；Phytophthora kind, such as phytophthora brown rot of citrus
(Phytophthora citrophthora), strawberry phytophthora (Phytophthora fragariae), Phytophthora cactorum
(Phytophthora cactorum), Phytophthora nicotianae (Phytophthora parasitica);Septoria (Septoria
), such as Septoria depressa spp.；Mucor (Mucor spp.), such as pyriform Mucor (Mucor
piriformis)；Chain sclerotinia sclerotiorum belong (Monilinia spp.), such as the raw chain sclerotinia sclerotiorum (Monilinia of fruit
Fructigena), drupe chain sclerotinia sclerotiorum (Monilinia laxa)；Venturia (Venturia spp.), such as the black star of apple
Bacterium (Venturia inaequalis), Pear scab (Venturia pyrina)；Rhizopus (Rhizopus spp.), such as
Rhizopus stolonifer (Rhizopus stolonifer), Rhizopus oryzae (Rhizopus oryzae)；It is small from shell category (Glomerella
Spp.), such as enclose small from shell (Glomerella cingulata)；Sclerotinia (Sclerotinia spp.), such as fruit are raw
Sclerotinite (Sclerotinia fruiticola)；Long beak shell category (Ceratocystis spp.), such as unusual long beak shell
(Ceratocystis paradoxa)；Penicillium (Penicillium spp.), such as penicillium funiculosum (Penicillium
Funiculosum), penicillium expansum (Penicillium expansum), Penicillium digitatum (Penicillium digitatum),
Italian mould (Penicillium italicum)；The long spore category of disk (Gloeosporium spp.), such as the long spore of white disk
(Gloeosporium album), Gloeosporium perennans, the raw long spore (Gloeosporium of disk of fruit
fructigenum),Gloeosporium singulata；Shell snake spore category (Phlyctaena spp.), such as Phlyctaena
vagabunda；Cylindrocarpon (Cylindrocarpon spp.), such as apple column spore (Cylindrocarpon mali)；It crawls handle
Mould category (Stemphyllium spp.), such as day lily stemphylium botryosum (Stemphyllium vesicarium)；Star check shell spore
Belong to (Phacydiopycnis spp.), such as Phacydiopycnis malirum；Thiclaviopsis (Thielaviopsis
Spp.), such as singular root string strain is mould (Thielaviopsis paradoxy)；Aspergillus (Aspergillus spp.) is such as black
Aspergillus (Aspergillus niger), Aspergillus carbonerius (Aspergillus carbonarius);Nectria (Nectria
), such as the red shell bacterium of dry cancer clump (Nectria galligena) spp.；Stockless Peziza (Pezicula spp.).
Bactericidal composition of the invention can also be used to prevent and treat fruit vegetables storing period disease, and obtain unexpected collaboration
The effect of synergy.Such as the fruit rot as caused by following pathogen:
Phytophthora (Phytophthora), such as phytophthora infestans (Phytophthorainfestans), phytophthora sojae kaufmann&gerdemann
(Phytophthoramegasperma), foot rot of citrus bacterium (Phytophthoraparasitica)；
Peronosporaceae (Peronosporaceae) disease such as Plasmopara viticola (Plasmoparaviticola), Peronospora disease
Bacterium (Peronospora)；
Pythium (Pythium) such as scraping and printing unit (Pythiumaphanidermatum).
After bactericidal composition according to the present invention can also being applied when plant or plant part grow to protect harvest
Stock.
Processing method according to the present invention can also be used for the invasion for protecting stock from fungi and microorganism.According to this hair
It is bright, it is understood as referring to term " stock " to have originated from natural life cycle and it is desirable that the plant of long-term preservation or animality is come
The natural materials in source and its processed form.Stock, such as plant of plant origin or part thereof, as stem, leaf, stem tuber,
Seed, fruit or seed, can be with the state of fresh harvesting or with form processing such as (pre-) dry, wetting, crushing, grinding, pressure
System or baking are protected.It is also possible to timber, thick timber form such as construction timber, electric pole and fence；Or final product form, such as by
Furniture or article made of timber.The stock of animal origin is animal skin, leather, hair, hair etc..Composition according to the present invention
It can prevent the invasion such as burn into colour fading or mouldy of the fungi or bacterium of storage period.It is preferred that " stock " is understood as referring to plant
The natural materials in object source and its form processing, more preferable fruit and its form processing, as the operatic circle, drupe, soft fruit and
Citrus fruit and its form processing.
The present invention provides a kind of plant for controlling plant, plant part, plant propagation material and the plant organ then grown
The method of object disease fungus, this method include by bactericidal composition of the invention so that agronomy is effective and basic plant-less toxicity is applied
Dosage applied with seed treatment, foliage applying, stem, is impregnated with, instiled, is poured, sprayed, is sprayed, dusting, the methods of distribution or smoke
Plant, plant part, plant propagation material or plant is administered to be growing or need in the soil or cultivation matchmaker wherein grown
In Jie.
Bactericidal composition of the invention can be applied by different processing methods, these methods for example:
Liquid comprising the bactericidal composition is sprayed onto the aerial part of the plant；
Dusting mixes particle or powder in the soil, sprays in the surrounding plants, and the tree injection or smearing the case where
Under；
Cladding or film coated are carried out to the seed of plant.
When for postharvest fruit and vegetable anti-corrosive fresh-keeping, 200-2000 times of liquid is usually diluted with water, is leached after soaking fruit.
The present invention provides a kind of method for preventing and treating plant pathogen, can be treatment, prevention or method of eradication.
Bactericidal composition of the invention can be made into common dosage form, such as missible oil, wettable powder, suspending agent, liquid
The dosage forms such as agent, granule, seed coat agent use, amount of application, according to the mixing ratio, meteorological condition, medicament of effective component
Form, Dressing date, method of administration, application place, prevent and kill off object noxious livings, objective crop etc. it is different and variant.
A method of prevention and treatment plant pathogen, amisulbrom and Fenamidone are administered simultaneously or respectively application or
Sequential application.
A method of prevention and treatment plant pathogen, including by bactericidal composition of the invention with agronomy effectively and substantially without plant
The amount of application of object toxicity applied with seed treatment, foliage applying, stem, is impregnated with, instiled, is poured, sprayed, is sprayed, dusting, distribution or
The methods of smoke is administered to plant pathogen and/or its environment or plant, plant part, plant propagation material and subsequent length
In plant organ out, soil or cultivation medium, material or space.
A method of the bactericidal composition is acted on plant or plant part by prevention and treatment plant pathogen.
A method of prevention and treatment plant pathogen, can plant by before infecting or after infecting by the Bactericidal composite
Object acts on plant pathogen and/or its environment or plant, plant part, plant propagation material and the plant then grown
In organ, soil or cultivation medium, material or space.
There may be super plus (" collaboration ") effects for processing according to the present invention.For example, the sterilization group used according to the present invention
It closes the rate of application of object and/or widens its field of activity and/or increase its activity, it is possible to obtain following effect: better plant
Growth increases the tolerance of high temperature or low temperature, increases the tolerance of arid or water or soil salt content, blooming performance mentions
It is high, it is easier to harvest, the maturation of quickening, higher yield rate, bigger fruit, higher plant height, the color of leaf is more
It is green, it blooms earlier, the quality or nutritive value of the product of harvest are higher, and sugared concentration is higher in fruit, the storage of the product of harvest
More preferably, these benefits have been more than the effect actually estimated for stability and/or processability.
Processing method of the invention can also be used in handle propagation material such as stem tuber or rhizome, and can be used for handling seed,
Seedling or transplanting (pricking out) seedling and plant or transplanting plant.The processing method can also be used for processing root.The present invention
Processing method can also be used for the aerial part of processing plant such as dry, stem or stalk, leaf, flower and fruit in relation to plant.
Generally for leaf portion processing: 0.1-10000g/ha, preferably 10-1000g/ha, more preferable 10-750g/ha；For
It irrigates or is added dropwise for application, the dosage can also even reduce, especially when application inert base such as asbestos or perlite
Shi Shi；
For seed treatment: 2-250g/100kg seed, preferably 3-200g/100kg seed, more preferable 2.5-50g/100kg kind
Son, even more preferably 5-25g/100kg seed.
For soil or processing used for ponds: 0.1-10000g/ha, preferably 1-5000g/ha.
It is fresh-keeping for postharvest fruit and vegetable, 200-2000 times of liquid can be diluted, is leached after soaking fruit.
Above-mentioned dosage is only general exemplary dose, and those skilled in the art can be according to practical feelings when reality is applied
Condition and needs adjust rate of application especially in accordance with the seriousness of the property and germ of plant to be processed or crop.
Amisulbrom of the invention and Fenamidone combination/combined administration.Including separating, sequentially or simultaneously applying indazole
Flusulfamide and Fenamidone.Preferably, the amisulbrom and Fenamidone group are combined into comprising amisulbrom and imidazoles bacterium
The form of the composition of ketone.
Composition of the invention can be based on dosage form, i.e., each substance has mixed in composition, composition at
Dividing can also be provided with unit dose form, mixed in bucket or tank using preceding, be then diluted to required concentration.Wherein preferably with this hair
Based on the dosage form of bright offer.
As a further improvement of the present invention, bactericidal composition of the invention can be configured to any agent agriculturally allowed
Type.
As a further improvement of the present invention, the dosage form of bactericidal composition of the invention be aerosol, it is capsule suspension, cold
Be atomized concentrating agents, heat atomization concentrating agents, CG/Encapsulated granule, granula subtilis, for seed treatment flowable concentrating agents, instant it is molten
Liquid, sprayable pulvis, emulsifiable concentrating agents, oil-in-water emulsion, water-in-oil emulsion, bulky grain agent, fine granule, oil can divide
Divided powder, oily miscible liquids, foaming agent, paste, suspension concentrating agents, solvable concentrating agents, is hanged the miscible flowable concentrating agents of oil
Floating agent, wettable powder, water dispersible granules, soluble powder, microcapsule suspending agent, coated granule, squeezes out particle at seed coat agent
Agent, missible oil, microemulsion, aqueous emulsion, effervescent tablet, ultra low volume liquids, suspoemulsion, the cold atomization preparation of ultra-low volume, ultra-low volume
Hot atomization preparation.
In bactericidal composition of the present invention, containing active constituent amisulbrom and Fenamidone, filler and/or
Surfactant.
Bactericidal composition of the present invention, wherein the content of amisulbrom and Fenamidone accounts for bactericidal composition
5%-90%。
The bactericidal composition, wherein the content of amisulbrom and Fenamidone accounts for the 10%-80% of bactericidal composition.
The bactericidal composition, wherein the content of amisulbrom and Fenamidone accounts for the 10%-60% of bactericidal composition.
According to the present invention, term " filler ", which refers to, can be combined or be combined with reactive compound to make it easier for being administered to
The natural or synthetic organic or inorganic compound of object (such as plant, crop or careless class).Therefore, the filler is preferably
It is inert, it is acceptable at least to should be agricultural.The filler can be solid or liquid.
The nonactive medium that can be used in the present invention can be used as solid matchmaker either solid is also possible to liquid
What dielectric material used has for example: phyteral powdery type (such as soy meal, starch, grain dust, wood powder, bark powder, sawdust, walnut
Shell powder, wheat bran, cellulose powder, coconut husk, corncob and tobacco stem particle, the residue etc. after extracting plant essence), paper
It opens, sawdust, crushes synthesized polymer body, the clay class (such as kaolin, bentonite, acid china clay etc.), talcum powder of synthetic resin etc.
Class.Silica (such as diatomite, silica sand, mica, aqueous silicic acid, calcium silicates), active carbon, natural mineral matter class (float stone, Lv Po
Thread stone and zeolite etc.), fire diatomite, sand, (such as polyethylene, polypropylene, the polyvinylidene chloride etc.), chlorination such as plastics medium
Chemical fertilizer, the soil of the inorganic mineral powder of potassium, calcium carbonate, calcium phosphate etc., ammonium sulfate, ammonium phosphate, urea, ammonium chloride etc.
Fertilizer, these substances can be used alone or two or more is mixed.
It can be used as can selecting in llowing group of materials for liquid media materials'use, such as water, alcohol type (such as first
Alcohol, ethyl alcohol, isopropanol, butanol, ethylene glycol etc.), ketone (such as acetone, methyl ethyl ketone, isobutyrone, cyclohexanone
Deng), ethers (such as ether, dioxanes, methylcellulose, tetrahydrofuran etc.), aliphatic hydrocarbon class (such as kerosene,
Mineral oil etc.), aromatic hydrocarbons class (such as benzene,toluene,xylene, solvent naphtha, alkylnaphthalene, chlorinated aromatic hydrocarbons, chloro rouge
Fat hydrocarbon, chlorobenzene, etc.), halogenated hydrocarbon class, amides, sulfone class, dimethyl sulfoxide, mineral and vegetable oil, animal oil etc..
To make the emulsification of effective component compound, dispersion and/or wetting, surfactant can be used for example can be with
Enumerate fatty alcohol polyoxyethylene ether, polyoxyethylene alkylaryl ether, polyoxyethylene higher fatty acid esters, polyoxyethylene alcohol or phenol
Phosphate, the aliphatic ester of polyalcohol, alkane aromatic sulfonic acid, naphthalene sulfonic acid polymer, lignosulfonates, the branch shape of macromolecule comb shape are total
Polymers, butyl naphthalene sulfonate, alkylaryl sulfonates, sodium alkylsulfosuccinates, grease, fatty alcohol and ethylene oxide condensation
The polyacrylates such as object, alkyltaurate, protein hydrolysate.Suitable oligosaccharide object or polymer, such as based on independent
Vinyl monomer, acrylic acid, polyoxyethylene and/or polyoxypropylene or itself and such as (polynary) alcohol or (polynary) amine combination.
To make effective component compound dispersion stability, attachment and/or combination, such as xanthan gum, silicic acid can be used
Magnalium, gelatin, starch, methyl cellulose, polyvinyl alcohol, polyvinyl acetate and natural phospholipid (such as cephalin and lecithin) with
And the adjuvants such as synthetic phospholipid, bentonite, sodium lignin sulfonate.
The wherein optional spent glycol of antifreezing agent, propylene glycol, glycerine, sorbierite.Deflocculant as suspension product
The adjuvant such as naphthalene sulfonic acid polymer, polymeric phosphate can be used.
Organic silicon defoamer can be used as defoaming agent.
The colorant that can be used, such as inorganic pigment, such as iron oxide, titanium oxide and Prussian blue；And organic pigment/
Dyestuff: alizarin dyes, azo dyes and metallized phthalocyanine dye；And microelement, such as molysite, manganese salt, boron salt, mantoquita, cobalt
Salt, molybdenum salt and zinc salt.
Optionally, also may include other annexing ingredients, for example, protecting colloid, adhesive, thickener, thixotropic agent, bleeding agent,
Stabilizer, screening agent.
The reactive compound can be mixed preparation with conventional additives by known way by the preparation of the invention.
The conventional additives such as conventional extender and solvent or diluent, emulsifier, dispersing agent, and/or adhesive or fixative,
Wetting agent, waterproofing agent, if it is desired, can also include drier and colorant, stabilizer, pigment, defoaming agent, preservative, increasing
Thick dose, water and other processing aids.
These compositions not only include can or dusting equipment such as spraying by suitable equipment be suitable for immediately it is to be processed
Object, but also including needing the concentration commercial composition being diluted before being applied to object.
Of the invention can also apply containing amisulbrom and Fenamidone with other active ingredient combinations, such as expanding
Big activity profile prevents from forming resistance.Other active constituents for example fungicide, bactericide, attractant, insecticide,
Acaricide, nematicide, growth regulator, herbicide, safener, fertilizer or semiochemical etc..
Active constituent in bactericidal composition of the present invention can also with itself or with its dosage form with it is known antifungal
Agent, bactericide, acaricide, nematicide or insecticide are used in mixed way, for example to widen its action spectrum or prevent resistance
It generates.
Fungicide:
1. nucleic acid synthetic inhibitor
M 9834 (benalaxyl), smart M 9834 (benalaxyl-M), the phonetic bacterium of sulphur clever (bupirimate), chiralaxyl,
Clozylacon, Milcurb (dimethirimol), Milstem (ethirimol), Furalaxyl (furalaxyl), hydroxyisoxazole
(hymexazole), Metalaxyl-M (metalaxyl-M), fenfuram (ofurace), Wakil (oxadixyl), oxolinic acide
(oxolinic acid)；
2. mitosis and cell division inhibitor
Benomyl (benomyl), carbendazim (carbendazim), diethofencarb (diethofencarb), furidazol
(fuberidazole), Pencycuron (pencycuron), Apl-Luster (thiabendazole), thiophanate methyl
(thiophanate-methyl), zoxamide (zoxamide)；
3. respiratory chain inhibitor
3.1 composite I
Fluorine mepanipyrim (diflumetorim)；
3.2 Complex II
Boscalid (boscalid), carboxin (carboxin), first furan drink aniline (fenfuram), flutolanil
(flutolanil), the spirit of furan pyrazoles (furametpyr), the third oxygen mebenil (mepronil), oxycarboxin
(oxycarboxin), pyrrole metsulfovax (penthiopyrad), thifluzamide (thifluzamide)；
3.3 Complex II I
Fluoxastrobin (azoxystrobin), cyazofamid (cyazofamid), dimoxystrobin (dimoxystrobin), enestrobin,
Famoxate (famoxadone), Fenamidone (fenamidone), fluoxastrobin (fluoxastrobin), imines bacterium
(kresoxim-methyl), phenalgin amide (metominostrobin), orysastrobin (orysastrobin), pyrazoles ether bacterium are pitched
Ester (pyraclostrobin), ZEN 90160 (picoxystrobin)；
3.4 uncoupler
Dinocap (dinocap), fluazinam (fluazinam)；
3.5ATP generates inhibitor
Fentinacetate (fentin acetate), Fentin chloride (fentin chloride), fentin hydroxide (fentin
Hydroxide), Silthiopham (silthiofam)；
4. amino acid and protein biosynthesis inhibitor
Amine puts out (andoprim), blasticidin-S (blasticidin-S), cyprodinil (cyprodinil), kasugarnycin
(kasugamycin), one hydrochloride hydrate of kasugarnycin (kasugamycin hydrochloride hydrate), mepanipyrim
(mepanipyrim), pyrimethanil (pyrimethanil)；
5. signal transduction inhibitor
Fenpiclonil (fenpiclonil), fluorine dislike bacterium (fludioxonil), quinoxyfen (quinoxyfen)；
6. lipid and film synthetic inhibitor
Chlozolinate (chlozolinate), isopropyl fixed (iprodione), sterilization benefit (procymidone), vinclozolin
(vinclozolin)；
Ppyrazophos (pyrazophos), Hinosan (edifenphos), different rice blast net (iprobenfos) (IBP), Isoprothiolane
(isoprothiolane)；
7. ergosterol biosynthesis inhibitor
Fenhexamid (fenhexamid), penta ring azoles (azaconazole), Bitertanol (bitertanol), bromuconazole
(bromuconazole), cyproconazole (cyproconazole), diclobutrazol (diclobutrazole), olefin conversion
(duniconazole), olefin conversion-M (diniconazole-M), oxole bacterium (epoxiconazole), etaconazole
(etaconazole), benzoxazole (fenbuconazole), Fluquinconazole (fluquinconazole), Flusilazole
(flusilazole), Flutriafol (flutriafol), furconazole (furconazole), furconazole_cis (furconazole-cis),
Hexaconazole (hexaconazole), amide azoles (imibenconazole), cycltebuconazole (ipconazole), penta azoles bacterium of ring
(metconazole), nitrile bacterium azoles (myclobutanil), paclobutrazol (paclobutrazol), penconazole (penconazole),
Propiconazole (propiconazole), prothioconazoles (prothioconazole), simeconazoles (simeconazole), Tebuconazole
(tebuconazole), fluorine ether azoles (tetraconazole), triazolone (triadimefon), triadimenol (triadimenol),
Triticonazole (triticonazole), uniconazole P (uniconazole), voriconazole (voriconazole), imazalil
(imazalil), enzyme (imazalil sulphate), evil imidazoles (oxpoconazole), fenarimol
(fenarimol), flurprimidol (flurprimidol), nuarimol (nuarimol), pyrifenox (pyrifenox), piperazine ammonia
Spirit (triforine), pefurazoate (pefurazoate), Prochloraz (prochloraz), fluorine bacterium azoles (triflumizole), alkene
White benzene azoles (viniconazole), dodemorph (dodemorph), dodemorph acetate (dodemorph
Acetate), butadiene morpholine (fenpropimorph), tridemorph (tridemorph), fenpropidin (fenpropidin), spiral shell are disliked
Luxuriant amine (spiroxamine), Naftifine (naftifine), pyributicarb (pyributicarb), Terbinafine
(terbinafine)；
8. Cell wall synthesis inhibitor
Benzene metsulfovax (benthiavalicarb), bialaphos (bialaphos), dimethomorph (dimethomorph), fluorine
Quinoline (flumorph), polyoxin (polyoxins), Polyoxin (polyoxorim), has iprovalicarb (iprovalicarb)
Validamycin A (validamycin A)；
9. melanin biosynthesis inhibitor
Ring propionyl bacterium amine (capropamid), double chlorine zarilamid (diclocymet), fenoxanil (fenoxanil), tetrachlorobenzene
Phthalein (phthalide), pyroquilon (pyroquilon)；
10. resistance inductors
Diazosulfide (acibenzolar-S-methyl), probenazole (probenazole), tiadinil (tiadinil)；
11. having the active compound of multidigit point
Difoltan (captafol), Bravo (chlorothalonil), mantoquita be for example: gram bacterium is strong, copper naphthenate, COPPER OXYCHLORIDE 37,5
(copper oxychloride), copper sulphate, copper monoxide, copper 8-hydroxyquinolinate (oxine copper), Bordeaux mixture, Euparen
(dichlofluanid), dithianon (dithianon), dodine (dodine), dodine free alkali (dodine free
Base), fervam (ferbam), fluorine folpet (fluorofolpet), folpet (folpet), fosetyl (fosetyl-
Al), the pungent salt of biguanides (guazatine), guazatine acetate (guazatine acetate), iminoctadine
(iminoctadine), double eight guanidine salts (iminoctadine albesilate), iminoctadine triacetate (iminoctadine
Triacetate), mancopper (mancopper), Mancozeb (mancozeb), maneb (maneb), Carbatene
(metiram), Carbatene zinc (metiram-zinc), Propineb (propineb), sulphur and sulphur preparation include calcium polysulfide, Fu Mei
Bis- (thiram), tolyfluanid (tolylfluanid), zineb (zineb), ziram (ziram)
12. unknown mechanism compound
Benthiozole (benthiazole), capsimycin (capsimycin), carvol (carvone), chinomethionat
(chinomethionat), chloropicrin (chloropicrin), cufraneb (cufraneb), Fenamidone
(cyflufenamid), cymoxanil (cymoxanil), dazomet (dazomet), debacarb (debacarb), diclomezin
(diclomezine), antiphen (dichlorophen), botran (dicloran), difenzoquat (difenzoquat), wild swallow
Withered methylsulfuric acid ester (difenzoquat methyl sulphate), diphenylamines (diphenylamine), Han Lening
(ethaboxam), ferimzone (ferimzone), fluorine acyl bacterium amine (flumetover), flusulfamide (flusulfamide), fluorine pyridine
Acyl bacterium amine (fluopicolide), fluoromide (fluoroimide), hexachloro-benzene (hexachlorobenzene), 8- hydroxyl
Quinoline, human world mycin (irumamycin), methasulfocarb (methasulfocarb), metrafenone (metrafenone), isothiocyanic acid
Methyl esters (methyl isothiocyanate), midolthromycin (mildiomycin), natamycin (natamycin), Sankel
(nickeldimethyldithiocarbamate), nitrothalisopropyl (nitrothal-isopropyl), different thiophene bacterium ketone
(octhilinone), oxamocarb, oxyfenthiin, pentachlorophenol (pentachlorophenol) and its salt, 2- phenyl
Phenol and its salt, pipron (piperalin), propanosine-sodium, the third oxygen quinoline (proquinazid), pyrroles bacterium
Element (pyrrolnitrin), pentachloronitrobenzene (quintozene), tecloftalam (tecloftalam), tecnazene
(tecnazene), azoles bacterium piperazine (triazoxide), trichlamide (trichlamide), zarilamid (zarilamid)；
Bactericide:
Bronopol (bronopol), antiphen, trichloromethyl pyridine (nitrapyrin), good fortune magnesium nickel (nickel
Dimethyldithiocarbamate), kasugarnycin, different thiophene bacterium ketone (octhilinone), carboxylic acid furans
(furancarboxylic acid), terramycin (oxytetracyclin), probenazole, streptomysin (streptomycin), leaf
Withered phthalein, copper sulphate and other copper agents.
Reactive compound amisulbrom and Fenamidone can be administered simultaneously, or apply respectively or sequential application, separately apply
The sequence of used time is on the result of prevention and treatment usually without influence.
Bactericidal composition of the invention can be based on dosage form, i.e., each substance has mixed in composition, composition
Ingredient can also be provided with unit dose form, mixed in bucket or tank using preceding, be then diluted to required concentration.Wherein preferably with
Based on dosage form provided by the invention.
For bactericidal composition of the invention in the case where reducing reactive compound application total amount, having to harmful fungoid improves activity
(synergy).And bactericidal composition of the invention shows that the bacterium of tolerance also has excellent kill to existing fungicide
Bacterium effect.
A kind of bactericidal composition of the present invention, the composition is by combining amisulbrom and Fenamidone, so that obtaining
Composition there is gain effect in control efficiency, and expanded fungicidal spectrum, played the multi-purpose effect of a medicine, effectively subtract
Delay or germ is avoided to develop drug resistance.Astoundingly, the bactericidal activity of bactericidal composition of the invention is than each active ingredient
The active adduction of object is considerably higher, and there are unpredictable, necessary being synergistic effects, rather than just active increasing
It mends.When reactive compound is present in bactericidal composition of the invention with specific weight ratio, synergistic effect is particularly evident.But
It is that the weight ratio of the reactive compound in bactericidal composition of the present invention can change in a certain range.
Bactericidal composition of the present invention is also surprising with plant physiology effect other than antifungal synergistic effect
Effect.The plant physiology includes:
Abiotic stress resistance, including after temperature-resistant, drought tolerance and drought stress recovery, water application efficiency is (with water consumption
Reduce related), flood resistance, ozone stress and UV resistance, to chemical substance such as heavy metal classes, salt, insecticide (safety
Agent) etc. resistance.
Biotic resistance, including increased fungus resistant and the increased resistance for being directed to nematode, virus and bacterium.
Increased plant vigor, including plant health/plant quality and seed vitality, the appearance of reduction toppled over, improved,
The increased afforestation effect for restoring, improving and improved photosynthetic efficiency.
The present invention provides a kind of bactericidal composition, the composition is by carrying out group for amisulbrom and Fenamidone
It closes, so that obtained composition has gain effect in control efficiency, and has expanded fungicidal spectrum, play what a medicine was mostly used
Effect, effectively slows down or germ is avoided to develop drug resistance.Astoundingly, the bactericidal activity of bactericidal composition of the invention is than each
The active adduction of a reactive compound is considerably higher.In other words, there are unpredictable, necessary being synergistic effects, and
Not only active supplement.
Specific embodiment
Below with reference to embodiment, the invention will be further described:
Example of formulations
+ 5% Fenamidone wettable powder of 1 10% amisulbrom of embodiment
Amisulbrom 10%
Fenamidone 5%
Lauryl sodium sulfate 6%
Sodium lignin sulfonate 5%
Attapulgite complements to 100%
Active constituent, various auxiliary agents and filler etc. are mixed by the proportional components of formula, arrived after ultra-fine pulverizer disintegrating
+ 5% Fenamidone wettable powder of 10% amisulbrom.
+ 2.5% Fenamidone missible oil of 2 2.5% amisulbrom of embodiment
Amisulbrom 2.5%
Fenamidone 2.5%
Ethoxylated castor oil 5%
Calcium dodecyl benzene sulfonate 3%
N-Methyl pyrrolidone complements to 100%
Mentioned component is proportionally prepared, uniform phase is uniformly mixing to obtain.
+ 6% Fenamidone water dispersible granules of 3 18% amisulbrom of embodiment
Amisulbrom 18%
Fenamidone 6%
MODIFIED LIGNOSULPHONATE 1%
Lauryl sodium sulfate 1%
Urea 1%
It is attapulgite modified to complement to 100%
Amisulbrom, Fenamidone active constituent, dispersing agent, wetting agent, disintegrating agent and filler are mixed according to the formula
Uniformly, by air-flow crushing at wettable powder；It adds a certain amount of water mixing extruding and makes material.It is obtained after dry screening
+ 6% Fenamidone water dispersible granules of 18% amisulbrom.
+ 10% Fenamidone coated granule of 4 5% amisulbrom of embodiment
Amisulbrom 5%
Fenamidone 10%
Polyethylene glycol 3%
The silicic acid 1% of high degree of dispersion
Calcium carbonate complements to 100%
It in a mixer, will be on levigate active constituent even spread to the carrier soaked by polyethylene glycol.It can obtain by this method
Obtain dust-free coated granules agent.
+ 5% Fenamidone of 5 5% amisulbrom of embodiment squeezes out granule
Amisulbrom 5%
Fenamidone 5%
Sodium lignin sulfonate 4%
Carboxymethyl cellulose 2%
White carbon black complements to 100%
Active component is mixed and ground with auxiliary agent, composition is soaked with water.The composition is squeezed out, is then done in the air stream
It is dry.
+ 6.7% Fenamidone suspension seed-coating agent of 6 33.3% amisulbrom of embodiment
Amisulbrom 33.3%
Fenamidone 6.7%
Fatty alcohol polyoxyethylene ether sulfosuccinic acid monoesters disodium 5%
MODIFIED LIGNOSULPHONATE 5%
Xanthan gum 1%
Hydroxyethyl cellulose 1%
Glycerine 5%
PVP-K30 1%
Water complements to 100%
Seed coat agent is obtained after above-mentioned each component is mixed ground and/or high speed shear in proportion.
Oil-suspending agent can be dispersed in+20% Fenamidone of 7 10% amisulbrom of embodiment
Amisulbrom 10%
Fenamidone 20%
Fatty alcohol polyoxyethylene ether sulfosuccinic acid monoesters disodium 5%
MODIFIED LIGNOSULPHONATE 5%
Xanthan gum 1%
Bentonite 1%
Glycerine 5%
PVP-K30 1%
Water 3%
Soybean oil complements to 100%
Dispersible oil-suspending agent is obtained after above-mentioned each component is mixed ground and/or high speed shear in proportion.
+ 18% Fenamidone suspending agent of 8 6% amisulbrom of embodiment
| Amisulbrom | 6% |
| Fenamidone | 18% |
| Methyl naphthalene sulfonate formaldehyde condensate | 5% |
| Dioctyl succinate disulfonate acid | 5% |
| Xanthan gum | 0.3% |
| Glycerine | 5% |
| Defoaming agent | 0.6% |
| Water | Complement to 100% |
The each components such as active component, dispersing agent, wetting agent and water are uniformly mixed according to the ratio of formula, it is ground and/or high
Speed shearing controls partial size at 3 μm hereinafter, obtaining+18% Fenamidone suspending agent of 6% amisulbrom.
+ 2.5% Fenamidone electrostatic oil agent of 9 1.5% amisulbrom of embodiment
Amisulbrom 1.5%
Fenamidone 2.5%
Ethoxylated castor oil 2%
Calcium dodecyl benzene sulfonate 3%
SOLVESSOTM100 complement to 100%
Above-mentioned each component is mixed, stirring is to obtaining transparent homogeneous phase.
+ 28% Fenamidone water dispersible granules of 10 7% amisulbrom of embodiment
Amisulbrom 7%
Fenamidone 28%
MODIFIED LIGNOSULPHONATE 1%
Lauryl sodium sulfate 1%
Ammonium sulfate 1%
It is attapulgite modified to complement to 100%
Amisulbrom, Fenamidone active constituent, dispersing agent, wetting agent, disintegrating agent and filler are mixed according to the formula
Uniformly, by air-flow crushing at wettable powder；It adds a certain amount of water mixing extruding and makes material.7% is obtained after dry screening
+ 28% Fenamidone water dispersible granules of amisulbrom.
+ 6% Fenamidone aqueous emulsion of 11 4% amisulbrom of embodiment
Oily phase:
| Amisulbrom | 4% |
| Fenamidone | 6% |
| Methyl oleate | 10% |
| Polystyrene | 3.5% |
Water phase:
| Xanthan gum | 0.1% |
| Naphthalene sulfonic acids-formaldehyde condensation products sodium salt of sulfonation | 1% |
| Fungicide | 0.2% |
| Water | Complement to 100% |
Amisulbrom and Fenamidone are dissolved in methyl oleate, polystyrene is added and obtains oily phase；According in formula
Component is uniformly mixed and obtains water phase；The oil phase is added to the aqueous phase under stiring obtains aqueous emulsion.
+ 3.7% Fenamidone microemulsion of 12 1.3% amisulbrom of embodiment
Amisulbrom 1.3%
Fenamidone 3.7%
Ethoxylated castor oil 5%
Calcium dodecyl benzene sulfonate 5%
SOLVESSOTM100 10%
Water complements to 100%
Above-mentioned each component is mixed, stirring is to obtaining transparent homogeneous phase.
+ 60% Fenamidone of 13 40% amisulbrom of embodiment
Amisulbrom 40%
Fenamidone 60%
Amisulbrom, Fenamidone are proportionally uniformly mixed.
+ 70% Fenamidone of 14 30% amisulbrom of embodiment
Amisulbrom 30%
Fenamidone 70%
Amisulbrom, Fenamidone are proportionally uniformly mixed.
+ 80% Fenamidone of 15 20% amisulbrom of embodiment
Amisulbrom 20%
Fenamidone 80%
Amisulbrom, Fenamidone are proportionally uniformly mixed.
Proportion is weight per distribution ratio in above embodiments.
Biological test example
One, virulence is tested:
The toxicity index of each medicament and the co-toxicity coefficient (CTC value) of mixture are calculated according to Sun Yunpei's Method, when CTC≤80, are then combined
Object shows antagonism, and when 80<CTC<120, then composition shows summation action, and when CTC>=120, then composition shows
Synergistic effect out.
Survey toxicity index (ATI)=(standard agent EC50/ reagent agent EC50) * 100
B in the percentage composition+B medicament toxicity index * mixture of A in theoretical toxicity index (TTI)=A medicament toxicity index * mixture
Percentage composition
Co-toxicity coefficient (CTC)=[mixture surveys toxicity index (ATI)/mixtures theoretical toxicity index (TTI) * 100
Phytopathogen and host plant used in the test of greenhouse virulence
| Host plant | Disease title | Genic organisms |
| Potato | The late blight of potato | Phytophthora infestans |
| Cucumber | Cucumber downy mildew | Cuba artificial downy mildew |
| Banana | Sigatoka | Sigatoka leaf spot disease |
| Wheat | Wheat powdery mildew | Grass family dlumeria graminis |
| Wheat | Wheat leaf rust | Puccinia recondita f. sp. tritici |
| Rice | Rice seedling damping-off | Fusarium graminearum |
| Cucumber | Powdery mildew of cucumber | Melon monofilament shell powdery mildew |
| Tomato | Graw mold of tomato | The pathogen of Botrytis cinerea |
| Chinese cabbage | Soft rot of cabbage | Carrot soft rot Erwinia |
| Corn | The leaf blight of corn | Big spot Exserohilum |
Test one: the toxicity test of phytophthora infestans
Using inhibition mycelial growth rate method:
Amisulbrom and Fenamidone are used into acetone solution respectively, then are configured to 0.1% Tween-80 aqueous solution dilution
The medical fluid of series of concentrations draws 6mL to the conical flask of sterilizing respectively in superclean bench, 50 DEG C or so of potato is added
Glucose agar medium (PDA) 54mL, pours into the plate of 4 diameter 9cm after shaking up, the toxic training of 4 respective concentrations is made
Support base；Toxic culture is made in the amisulbrom of different ratio and Fenamidone series of concentrations compound solution with same method
Base.2 days phytophthora infestans will be cultivated, breaks into fungus block in colony edge with the punch of diameter 5mm, moved fungus block with transfer needle
To the toxic PDA culture medium center being configured in advance, it is subsequently placed in culture, every processing in 25 DEG C of incubators and is repeated 4 times.After 3 days,
Each processing colony diameter cm is measured using crossing method slide calliper rule, correction is found out and inhibits percentage.Each bacterium colony right-angled intersection
Two diameters are surveyed, bacterium colony size is represented with its average.Then bacterium colony growth inhibition ratio is found out as the following formula:
Then concentration EC in inhibiting is calculated with least square method50, then according to Sun Yunpei's Method calculating co-toxicity coefficient (CTC).
Table 1: to the virulence test result of phytophthora infestans
As known from Table 1, when the combination of amisulbrom and Fenamidone is in the range for matching 25:1-1:8, to phytophthora infestans
Co-toxicity coefficient is all larger than 120, shows as synergistic effect.
Test two: the toxicity test of Cuba artificial downy mildew
Using inhibition mycelial growth rate method:
Amisulbrom and Fenamidone are used into acetone solution respectively, then are configured to 0.1% Tween-80 aqueous solution dilution
The medical fluid of series of concentrations draws 6mL to the conical flask of sterilizing respectively in superclean bench, 50 DEG C or so of potato is added
Glucose agar medium (PDA) 54mL, pours into the plate of 4 diameter 9cm after shaking up, the toxic training of 4 respective concentrations is made
Support base；Toxic culture is made in the amisulbrom of different ratio and Fenamidone series of concentrations compound solution with same method
Base.2 days Cuba artificial downy mildews will be cultivated, break into fungus block in colony edge with the punch of diameter 5mm, with transfer needle by fungus block
The toxic PDA culture medium center being configured in advance is moved to, culture, every processing in 25 DEG C of incubators is subsequently placed in and is repeated 4 times.3 days
Afterwards, each processing colony diameter cm is measured using crossing method slide calliper rule, finds out correction and inhibits percentage.Each bacterium colony cross is handed over
Fork surveys two diameters, represents bacterium colony size with its average.Then bacterium colony growth inhibition ratio is found out as the following formula:
Then concentration EC in inhibiting is calculated with least square method50, then according to Sun Yunpei's Method calculating co-toxicity coefficient (CTC).
Table 2: to the virulence test result of Cuba artificial downy mildew
As known from Table 2, when the combination of amisulbrom and Fenamidone is in the range for matching 25:1-1:10, to the false frost of Cuba
Mould co-toxicity coefficient is all larger than 120, shows as synergistic effect.
Experiment three: the indoor toxicity test of amisulbrom and Fenamidone to sigatoka leaf spot disease bacterium
Using inhibition mycelial growth rate method:
Amisulbrom and Fenamidone are used into acetone solution respectively, then are configured to 0.1% Tween-80 aqueous solution dilution
The medical fluid of series of concentrations draws 6mL to the conical flask of sterilizing respectively in superclean bench, 50 DEG C or so of potato is added
Glucose agar medium (PDA) 54mL, pours into the plate of 4 diameter 9cm after shaking up, the toxic training of 4 respective concentrations is made
Support base；Toxic culture is made in the amisulbrom of different ratio and Fenamidone series of concentrations compound solution with same method
Base.2 days sigatoka leaf spot disease bacterium will be cultivated, break into fungus block in colony edge with the punch of diameter 5mm, with transfer needle by fungus block
The toxic PDA culture medium center being configured in advance is moved to, culture, every processing in 25 DEG C of incubators is subsequently placed in and is repeated 4 times.3 days
Afterwards, each processing colony diameter cm is measured using crossing method slide calliper rule, finds out correction and inhibits percentage.Each bacterium colony cross is handed over
Fork surveys two diameters, represents bacterium colony size with its average.Then bacterium colony growth inhibition ratio is found out as the following formula:
Then concentration EC in inhibiting is calculated with least square method50, then according to Sun Yunpei's Method calculating co-toxicity coefficient (CTC).
Table 3: to the virulence test result of sigatoka leaf spot disease bacterium
As known from Table 3, when the combination of amisulbrom and Fenamidone is in the range for matching 25:1-1:25, to brown edge greyness
Germ co-toxicity coefficient is all larger than 120, shows as synergistic effect.
Experiment four: the indoor toxicity test of amisulbrom and Fenamidone to Fusarium graminearum
Using inhibition mycelial growth rate method:
Amisulbrom and Fenamidone are used into acetone solution respectively, then are configured to 0.1% Tween-80 aqueous solution dilution
The medical fluid of series of concentrations draws 6mL to the conical flask of sterilizing respectively in superclean bench, 50 DEG C or so of potato is added
Glucose agar medium (PDA) 54mL, pours into the plate of 4 diameter 9cm after shaking up, the toxic training of 4 respective concentrations is made
Support base；Toxic culture is made in the amisulbrom of different ratio and Fenamidone series of concentrations compound solution with same method
Base.2 days Fusarium graminearums will be cultivated, breaks into fungus block in colony edge with the punch of diameter 5mm, moved fungus block with transfer needle
To the toxic PDA culture medium center being configured in advance, it is subsequently placed in culture, every processing in 25 DEG C of incubators and is repeated 4 times.After 3 days,
Each processing colony diameter cm is measured using crossing method slide calliper rule, correction is found out and inhibits percentage.Each bacterium colony right-angled intersection
Two diameters are surveyed, bacterium colony size is represented with its average.Then bacterium colony growth inhibition ratio is found out as the following formula:
Then concentration EC in inhibiting is calculated with least square method50, then according to Sun Yunpei's Method calculating co-toxicity coefficient (CTC).
Table 4: to the virulence test result of Fusarium graminearum
As known from Table 4, when the combination of amisulbrom and Fenamidone is in the range for matching 25:1-1:25, to cereal reaping hook
Bacterium co-toxicity coefficient is all larger than 120, shows as synergistic effect.
Experiment five: the indoor toxicity test of amisulbrom and Fenamidone to melon monofilament shell powdery mildew
Using inhibition mycelial growth rate method:
Amisulbrom and Fenamidone are used into acetone solution respectively, then are configured to 0.1% Tween-80 aqueous solution dilution
The medical fluid of series of concentrations draws 6mL to the conical flask of sterilizing respectively in superclean bench, 50 DEG C or so of potato is added
Glucose agar medium (PDA) 54mL, pours into the plate of 4 diameter 9cm after shaking up, the toxic training of 4 respective concentrations is made
Support base；Toxic culture is made in the amisulbrom of different ratio and Fenamidone series of concentrations compound solution with same method
Base.2 days melon monofilament shell powdery mildews will be cultivated, breaks into fungus block in colony edge with the punch of diameter 5mm, it will with transfer needle
Fungus block moves to the toxic PDA culture medium center being configured in advance, is subsequently placed in culture, every processing in 25 DEG C of incubators and is repeated 4 times.
After 3 days, each processing colony diameter cm is measured using crossing method slide calliper rule, correction is found out and inhibits percentage.Each bacterium colony ten
Word, which intersects, surveys two diameters, represents bacterium colony size with its average.Then bacterium colony growth inhibition ratio is found out as the following formula:
Then concentration EC in inhibiting is calculated with least square method50, then according to Sun Yunpei's Method calculating co-toxicity coefficient (CTC).
Table 5: to the virulence test result of melon monofilament shell powdery mildew
As known from Table 5, when the combination of amisulbrom and Fenamidone is in the range for matching 25:1-1:25, to melon monofilament
Shell powdery mildew co-toxicity coefficient is all larger than 120, shows as synergistic effect.
Experiment six: the indoor toxicity test of amisulbrom and Fenamidone to the pathogen of Botrytis cinerea
Using inhibition mycelial growth rate method:
Amisulbrom and Fenamidone are used into acetone solution respectively, then are configured to 0.1% Tween-80 aqueous solution dilution
The medical fluid of series of concentrations draws 6mL to the conical flask of sterilizing respectively in superclean bench, 50 DEG C or so of potato is added
Glucose agar medium (PDA) 54mL, pours into the plate of 4 diameter 9cm after shaking up, the toxic training of 4 respective concentrations is made
Support base；Toxic culture is made in the amisulbrom of different ratio and Fenamidone series of concentrations compound solution with same method
Base.2 days the pathogen of Botrytis cinerea will be cultivated, breaks into fungus block in colony edge with the punch of diameter 5mm, moved fungus block with transfer needle
To the toxic PDA culture medium center being configured in advance, it is subsequently placed in culture, every processing in 25 DEG C of incubators and is repeated 4 times.After 3 days,
Each processing colony diameter cm is measured using crossing method slide calliper rule, correction is found out and inhibits percentage.Each bacterium colony right-angled intersection
Two diameters are surveyed, bacterium colony size is represented with its average.Then bacterium colony growth inhibition ratio is found out as the following formula:
Then concentration EC in inhibiting is calculated with least square method50, then according to Sun Yunpei's Method calculating co-toxicity coefficient (CTC).
Table 6: to the virulence test result of the pathogen of Botrytis cinerea
As known from Table 6, when the combination of amisulbrom and Fenamidone is in the range for matching 25:1-1:25, to Botrytis cinerea
Bacterium co-toxicity coefficient is all larger than 120, shows as synergistic effect.
Experiment seven: the indoor toxicity test of amisulbrom and Fenamidone to big spot Exserohilum bacterium
Using inhibition mycelial growth rate method:
Amisulbrom and Fenamidone are used into acetone solution respectively, then are configured to 0.1% Tween-80 aqueous solution dilution
The medical fluid of series of concentrations draws 6mL to the conical flask of sterilizing respectively in superclean bench, 50 DEG C or so of potato is added
Glucose agar medium (PDA) 54mL, pours into the plate of 4 diameter 9cm after shaking up, the toxic training of 4 respective concentrations is made
Support base；Toxic culture is made in the amisulbrom of different ratio and Fenamidone series of concentrations compound solution with same method
Base.2 days big spot Exserohilum bacterium will be cultivated, break into fungus block in colony edge with the punch of diameter 5mm, with transfer needle by bacterium
Block moves to the toxic PDA culture medium center being configured in advance, is subsequently placed in culture, every processing in 25 DEG C of incubators and is repeated 4 times.3
After it, each processing colony diameter cm is measured using crossing method slide calliper rule, correction is found out and inhibits percentage.Each bacterium colony cross
Intersect and survey two diameters, bacterium colony size is represented with its average.Then bacterium colony growth inhibition ratio is found out as the following formula:
Then concentration EC in inhibiting is calculated with least square method50, then according to Sun Yunpei's Method calculating co-toxicity coefficient (CTC).
Table 7: to the virulence test result of big spot Exserohilum bacterium
As known from Table 7, when the combination of amisulbrom and Fenamidone is in the range for matching 25:1-1:25, to the convex navel of big spot
Compacted spore bacterium co-toxicity coefficient is all larger than 120, shows as synergistic effect.
Experiment eight: the indoor toxicity test of amisulbrom and Fenamidone to carrot soft rot Erwinia
Using inhibition mycelial growth rate method:
Amisulbrom and Fenamidone are used into acetone solution respectively, then are configured to 0.1% Tween-80 aqueous solution dilution
The medical fluid of series of concentrations draws 6mL to the conical flask of sterilizing respectively in superclean bench, 50 DEG C or so of potato is added
Glucose agar medium (PDA) 54mL, pours into the plate of 4 diameter 9cm after shaking up, the toxic training of 4 respective concentrations is made
Support base；Toxic culture is made in the amisulbrom of different ratio and Fenamidone series of concentrations compound solution with same method
Base.2 days carrot soft rot Erwinias will be cultivated, breaks into fungus block in colony edge with the punch of diameter 5mm, use transfer needle
Fungus block is moved to the toxic PDA culture medium center being configured in advance, is subsequently placed in culture in 25 DEG C of incubators, every processing repeats 4
It is secondary.After 3 days, each processing colony diameter cm is measured using crossing method slide calliper rule, correction is found out and inhibits percentage.Each bacterium colony
Two diameters are surveyed in right-angled intersection, represent bacterium colony size with its average.Then bacterium colony growth inhibition ratio is found out as the following formula:
Then concentration EC in inhibiting is calculated with least square method50, then according to Sun Yunpei's Method calculating co-toxicity coefficient (CTC).
Table 8: to the virulence test result of carrot soft rot Erwinia
As known from Table 8, when the combination of amisulbrom and Fenamidone is in the range for matching 25:1-1:25, to carrot soft rot
Erwinia co-toxicity coefficient is all larger than 120, shows as synergistic effect.
Experiment nine: the indoor toxicity test of amisulbrom and Fenamidone to wheat powdery mildew
Using inhibition mycelial growth rate method:
Amisulbrom and Fenamidone are used into acetone solution respectively, then are configured to 0.1% Tween-80 aqueous solution dilution
The medical fluid of series of concentrations draws 6mL to the conical flask of sterilizing respectively in superclean bench, 50 DEG C or so of potato is added
Glucose agar medium (PDA) 54mL, pours into the plate of 4 diameter 9cm after shaking up, the toxic training of 4 respective concentrations is made
Support base；Toxic culture is made in the amisulbrom of different ratio and Fenamidone series of concentrations compound solution with same method
Base.2 days grass family dlumeria graminis will be cultivated, breaks into fungus block in colony edge with the punch of diameter 5mm, it will with transfer needle
Fungus block moves to the toxic PDA culture medium center being configured in advance, is subsequently placed in culture, every processing in 25 DEG C of incubators and is repeated 4 times.
After 3 days, each processing colony diameter cm is measured using crossing method slide calliper rule, correction is found out and inhibits percentage.Each bacterium colony ten
Word, which intersects, surveys two diameters, represents bacterium colony size with its average.Then bacterium colony growth inhibition ratio is found out as the following formula:
Then concentration EC in inhibiting is calculated with least square method50, then according to Sun Yunpei's Method calculating co-toxicity coefficient (CTC).
Table 9: to the virulence test result of grass family dlumeria graminis
As known from Table 9, when the combination of amisulbrom and Fenamidone is in the range for matching 25:1-1:25, to grass family cloth
The co-toxicity coefficient of family name's powdery mildew is all larger than 120, shows as synergistic effect.
Experiment ten: the indoor toxicity test of amisulbrom and Fenamidone to wheat leaf rust
Using inhibition mycelial growth rate method:
Amisulbrom and Fenamidone are used into acetone solution respectively, then are configured to 0.1% Tween-80 aqueous solution dilution
The medical fluid of series of concentrations draws 6mL to the conical flask of sterilizing respectively in superclean bench, 50 DEG C or so of potato is added
Glucose agar medium (PDA) 54mL, pours into the plate of 4 diameter 9cm after shaking up, the toxic training of 4 respective concentrations is made
Support base；Toxic culture is made in the amisulbrom of different ratio and Fenamidone series of concentrations compound solution with same method
Base.2 days Puccinia recondita f. sp. triticis will be cultivated, break into fungus block in colony edge with the punch of diameter 5mm, with transfer needle by bacterium
Block moves to the toxic PDA culture medium center being configured in advance, is subsequently placed in culture, every processing in 25 DEG C of incubators and is repeated 4 times.3
After it, each processing colony diameter cm is measured using crossing method slide calliper rule, correction is found out and inhibits percentage.Each bacterium colony cross
Intersect and survey two diameters, bacterium colony size is represented with its average.Then bacterium colony growth inhibition ratio is found out as the following formula:
Then concentration EC in inhibiting is calculated with least square method50, then according to Sun Yunpei's Method calculating co-toxicity coefficient (CTC).
Table 10: to the virulence test result of Puccinia recondita f. sp. tritici
As known from Table 10, when the combination of amisulbrom and Fenamidone is in the range for matching 25:1-1:25, wheat is hidden
The co-toxicity coefficient of handle rest fungus is all larger than 120, shows as synergistic effect.
Claims (16)
1. a kind of bactericidal composition, it is characterised in that: the bactericidal composition contains active constituent amisulbrom and epoxiconazole,
Wherein the weight percent of amisulbrom and epoxiconazole be 25:1-1:25, further preferably 20:1-1:20, further preferably for
15:1-1:15, more preferably 10:1-1:10, more preferably 5:1-1:5.
2. bactericidal composition according to claim 1, it is characterised in that: the amisulbrom and epoxiconazole quality sum
Account for the 5%-90% of the Bactericidal composite amount of substance, preferably 10%-80%, more preferable 20%-70%.
3. bactericidal composition according to claim 1, it is characterised in that: the bactericidal composition, dosage form be suspending agent,
Seed coat agent, wettable powder, water dispersible granules, microcapsule suspending agent, coated granule, squeezes out granule, is missible oil, micro- suspoemulsion
Emulsion, aqueous emulsion, effervescent tablet, ultra low volume liquids, dry suspension, electrostatic oil agent.
4. bactericidal composition according to claim 1, it is characterised in that: also include filler and/or surfactant.
5. a kind of method for preventing and treating plant pathogen, it is characterised in that: bactericidal composition described in claim 1 is acted on plant
Object pathogenic bacteria and/or its environment or plant, plant part, plant propagation material and the plant organ then grown, soil or
It cultivates in medium, material or space.
6. a kind of method for preventing and treating plant pathogen, it is characterised in that: by amisulbrom described in claim 1 and epoxiconazole
Be administered simultaneously or respectively application or sequential application.
7. a kind of method for preventing and treating plant pathogen, it is characterised in that: wanted before infecting or by right after infecting in plant
Bactericidal composition described in asking 1 acts on plant pathogen and/or its environment or plant, plant part, plant propagation material
In plant organ, soil or cultivation medium, material or the space then grown.
8. it is a kind of prevent and treat plant pathogen method, it is characterised in that: including by bactericidal composition described in claim 1 with agriculture
Learn the amount of application of effective and basic plant-less toxicity applied, be impregnated with seed treatment, foliage applying, stem, instiled, is poured, sprayed,
The methods of spraying, dusting, distribution or smoke are applied to plant pathogen and/or its environment or plant, plant part, plant
In propagation material and the plant organ then grown, soil or cultivation medium, material or space.
9. bactericidal composition described in claim 1 is true on Cereal, veterinary antibiotics, ornamental plant and grapevine for preventing and treating
The purposes of bacterium and bacterium.
10. bactericidal composition described in claim 1 is for controlling Cereal, veterinary antibiotics, silk on ornamental plant and grapevine
Pyrenomycetes category, Verticillium Nees, knee Pseudomonas, rotten mold genus, downy mildew Pseudomonas, phytophthora, Fusarium, grey mold Pseudomonas, white powder
Pseudomonas, hair mycosphaerella, Exserohilum category, Colletotrichum harmful fungoid purposes.
11. bactericidal composition described in claim 1 is for controlling downy mildew, late blight, early blight, leaf spot, banded sclerotial blight, ash
Mildew, powdery mildew, damping-off, soft rot, leaf blight, anthracnose, green mould, shot hole, spot defoliation, bacterial wilt
Disease, black spot, white beautiful disease, samping off, leaf mold, spot defoliation, brown spot, false smut, rice blast, head blight, rust
Purposes.
12. bactericidal composition described in claim 1 is for protecting plant, plant propagation material and the plant organ then grown
Purposes.
13. causing a disease in place prevention and treatment soil or cultivation medium that bactericidal composition described in claim 1 is prevented and treated needed for being applied to
Or the purposes of saprophytic fungi and bacterium.
14. bactericidal composition described in claim 1 is used to handle seed to protect the phytopathogen of seed carrying to invade
Purposes.
15. the purposes that bactericidal composition described in claim 1 is used to protect stock.
16. bactericidal composition described in claim 1 is for protecting stock in storage period from fungi or the use of bacterial invasion
On the way.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710556224.4A CN109221160A (en) | 2017-07-10 | 2017-07-10 | A kind of bactericidal composition |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710556224.4A CN109221160A (en) | 2017-07-10 | 2017-07-10 | A kind of bactericidal composition |
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| CN109221160A true CN109221160A (en) | 2019-01-18 |
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| Country | Link |
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| CN (1) | CN109221160A (en) |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1949970A (en) * | 2004-04-30 | 2007-04-18 | 巴斯福股份公司 | Fungicidal mixtures |
| JP2008303204A (en) * | 2007-01-05 | 2008-12-18 | Nissan Chem Ind Ltd | Fungicidal composition |
| JP2010150230A (en) * | 2008-05-09 | 2010-07-08 | Nissan Chem Ind Ltd | Germicidal composition |
-
2017
- 2017-07-10 CN CN201710556224.4A patent/CN109221160A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1949970A (en) * | 2004-04-30 | 2007-04-18 | 巴斯福股份公司 | Fungicidal mixtures |
| JP2008303204A (en) * | 2007-01-05 | 2008-12-18 | Nissan Chem Ind Ltd | Fungicidal composition |
| JP2010150230A (en) * | 2008-05-09 | 2010-07-08 | Nissan Chem Ind Ltd | Germicidal composition |
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