CN109207540A - The extracting method of protein in a kind of frozen meat thawing water - Google Patents
The extracting method of protein in a kind of frozen meat thawing water Download PDFInfo
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- CN109207540A CN109207540A CN201811007780.7A CN201811007780A CN109207540A CN 109207540 A CN109207540 A CN 109207540A CN 201811007780 A CN201811007780 A CN 201811007780A CN 109207540 A CN109207540 A CN 109207540A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/145—Extraction; Separation; Purification by extraction or solubilisation
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/30—Extraction; Separation; Purification by precipitation
- C07K1/303—Extraction; Separation; Purification by precipitation by salting out
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/34—Extraction; Separation; Purification by filtration, ultrafiltration or reverse osmosis
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- Proteomics, Peptides & Aminoacids (AREA)
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Abstract
The present invention discloses a kind of extracting method of protein in frozen meat thawing water, comprising the following steps: first separation, cleaning, secondary separation, pH value Primary regulation, enzymatic hydrolysis, pH value Secondary Control, flocculating setting, heating, neutralization and drying process；When first separation, by weight, take 8~10 parts of frozen meat thawing waters, hyperfiltration treatment is carried out by the ultrafiltration membrane of 1500 dalton of interception, when secondary separation, by the retention ingredient after above-mentioned cleaning, hyperfiltration treatment is carried out by the super worry film of 2000 dalton of interception, enzymatic hydrolysis, trapped substance by grind slurries quality than albumen complex enzyme and secondary separation for 0.35% is mixed with the ratio of 1:1, the extracting method of protein in frozen meat thawing water of the present invention, extract protein in frozen meat thawing water, solve pollution problem of the thawing water to environment, recycle the protein in frozen meat thawing water, reduce the loss of nutrients and the waste of resource.
Description
Technical field
The present invention relates to a kind of extracting method of protein, the extraction of protein in specifically a kind of frozen meat thawing water
Method.
Background technique
Frozen meat has become one of the vegetable on daily dining table, due to the difference of region, leads to partial region discomfort symphysis
Catch class batch cultivation, in order to meet demand of the consumer in this region to capturing, need to capture freezing after from supply ground
With being transported to demand, the meat product after freezing, during defrosting, as frozen meat thawing will lead to the stream of meat nutrition
It loses, containing amino acid and protein contained by meat in thawing water, the mode of common processing frozen meat thawing water is expelled directly out
Sewer, thawing water will lead to the pollution of environment, and lead to the loss of nutrients in thawing water, so that the waste of resource.
Summary of the invention
The purpose of the present invention is to provide a kind of extracting method of protein in frozen meat thawing water, by first separation,
At cleaning, secondary separation, pH value Primary regulation, enzymatic hydrolysis, pH value Secondary Control, flocculating setting, heating, neutralization and drying
Reason extracts protein in frozen meat thawing water, solves pollution problem of the thawing water to environment, reduce the loss of nutrients
With the waste of resource.
The purpose of the present invention can be achieved through the following technical solutions:
The extracting method of protein in a kind of frozen meat thawing water, comprising the following steps:
(1) first separation takes 8~10 parts of frozen meat thawing waters by weight, passes through 1500 dalton of interception
Ultrafiltration membrane carry out hyperfiltration treatment, the macromolecular substances such as protein therein are retained down from thawing water, collect retention at
Point.
(2) it cleans, retain ingredient and the distilled water after first separation is mixed with the ratio of 1:1, added in retention ingredient
It while entering distilled water, is added while stirring, and stirring is rectangular always consistent, after water to be distilled is added completely into, continues to stir
It mixes 4~5 minutes.
(3) secondary separation carries out the retention ingredient after above-mentioned cleaning by the super worry film of 2000 dalton of interception
First separation trapped substance after cleaning is retained down by hyperfiltration treatment from distilled water, collects retention ingredient.
(4) pH value Primary regulation will be added 30% sodium hydroxide solution, while use glass in the ingredient of above-mentioned retention
Stick stirring, carries out test paper comparison after stirring, adjust pH value to 6.5~7.5.
(5) enzymatic hydrolysis, the trapped substance by grind slurries quality than albumen complex enzyme and secondary separation for 0.35% is with 1:1
Ratio mixed, temperature is maintained at 50~55 degrees Celsius, and pH value is 7~8, hydrolyzes 3.5 hours.
(6) 1~2 part 30% of hydrochloric acid will be added by weight in pH value Secondary Control in the product after above-mentioned hydrolysis
Solution is stirred using glass bar, static 4~5 minutes after stirring, test paper comparison is carried out after stirring, continuously adds 1~2 part 30%
Hydrochloric acid solution, stirred, mixed within static 4~5 minutes after stirring, circulate operation using glass bar, until adjusting pH value to 4~4.5.
(7) 1~2 part 15% of hydrochloric acid will be added by weight in flocculating setting in the product after pH value Secondary Control
Solution while addition and is stirred continuously, and is stirred 2~3 minutes, and test paper comparison, circulate operation, to its pH value are carried out after stirring
It is finely adjusted, pH value is adjusted to 3.5~4.5, natural subsidence 7 hours, removes clear liquid.
(8) it heats, above-mentioned gained sediment is fitted into container, another is put it into and is placed in the container of aqueous solution,
The container for being placed with aqueous solution is heated, is heated to 40 degrees Celsius, keeps the temperature 30 minutes.
(9) it neutralizes, the above-mentioned container equipped with sediment is placed in 10~15 degrees Celsius of temperature of aqueous solution, by weight
Part meter is measured, 1~2 part 30% of sodium hydroxide solution is added into sediment, and is stirred continuously, it is 2~3 minutes exquisite, continue to add
Enter 1~2 part 30% of sodium hydroxide solution and stirring, until adjusting pH value to 6.5~7, obtains the slurries of protein.
(10) dry, sediment is dried using spray dryer, the temperature control of air inlet 180~
200 degrees Celsius, the slurries of protein are stirred while spraying, improve the speed of moisture evaporation, the temperature of air outlet is
80 degrees Celsius, to moisture evaporating completely, obtain dry protein raw material.
Further, in the first separation, the intake pressure of ultrafiltration membrane is controlled in 0.15~0.25 Pascal, temperature
It is 28~32 degrees Celsius.
Further, in the secondary separation, the intake pressure of ultrafiltration membrane is controlled in 0.12~0.20 Pascal, temperature
It is 28~32 degrees Celsius.
Further, the albumen complex enzyme includes alkali protease, lipase and trypsase.
Beneficial effects of the present invention:
1, in frozen meat thawing water of the present invention protein extracting method, pass through first separation, cleaning, secondary separation, PH
It is worth Primary regulation, enzymatic hydrolysis, pH value Secondary Control, flocculating setting, heating, neutralization and drying process, extracts frozen meat and melt
Protein in water solves pollution problem of the thawing water to environment；
2, in frozen meat thawing water of the present invention protein extracting method, obtain the protein in frozen meat thawing water
It recycles, reduces the loss of nutrients and the waste of resource.
Specific embodiment
The following is a clear and complete description of the technical scheme in the embodiments of the invention, it is clear that described implementation
Example is only a part of the embodiment of the present invention, instead of all the embodiments.Based on the embodiments of the present invention, this field is general
Logical technical staff all other embodiment obtained without creative efforts, belongs to protection of the present invention
Range.
In the description of the present invention, it is to be understood that, term " aperture ", "upper", "lower", " thickness ", "top", " in ",
Indicating positions or the positional relationship such as " length ", "inner", " surrounding ", are merely for convenience of description of the present invention and simplification of the description, without
It is that the component of indication or suggestion meaning or element must have a particular orientation, is constructed and operated in a specific orientation, therefore not
It can be interpreted as limitation of the present invention.
The extracting method of protein in a kind of frozen meat thawing water, comprising the following steps:
(1) first separation takes 8~10 parts of frozen meat thawing waters by weight, passes through 1500 dalton of interception
Ultrafiltration membrane carry out hyperfiltration treatment, the macromolecular substances such as protein therein are retained down from thawing water, collect retention at
Point, in 0.15~0.25 Pascal, temperature is 28~32 degrees Celsius for the intake pressure control of ultrafiltration membrane.
(2) it cleans, retain ingredient and the distilled water after first separation is mixed with the ratio of 1:1, added in retention ingredient
It while entering distilled water, is added while stirring, and stirring is rectangular always consistent, after water to be distilled is added completely into, continues to stir
It mixes 4~5 minutes.
(3) secondary separation carries out the retention ingredient after above-mentioned cleaning by the super worry film of 2000 dalton of interception
First separation trapped substance after cleaning is retained down by hyperfiltration treatment from distilled water, collect retention ingredient, ultrafiltration membrane into
In 0.12~0.20 Pascal, temperature is 28~32 degrees Celsius for water pressure control.
(4) pH value Primary regulation will be added 30% sodium hydroxide solution, while use glass in the ingredient of above-mentioned retention
Stick stirring, carries out test paper comparison after stirring, adjust pH value to 6.5~7.5.
(5) enzymatic hydrolysis, the trapped substance by grind slurries quality than albumen complex enzyme and secondary separation for 0.35% is with 1:1
Ratio mixed, albumen complex enzyme includes alkali protease, lipase and trypsase, and temperature is maintained at 50~55 and takes the photograph
Family name's degree, pH value are 7~8, are hydrolyzed 3.5 hours.
(6) 1~2 part 30% of hydrochloric acid will be added by weight in pH value Secondary Control in the product after above-mentioned hydrolysis
Solution is stirred using glass bar, and the purpose of stirring is to be uniformly mixed, and whole pH value synchronous change improves the speed of heat dissipation
Degree static 4~5 minutes after stirring, carries out test paper comparison, continuously adds 1~2 part 30% of hydrochloric acid solution, use glass after stirring
Glass stick stirs, and mixes within static 4~5 minutes after stirring, circulate operation, up to adjusting pH value to 4~4.5, the purpose of substep intermittent operation
It is after hydrochloric acid is added, is neutralization reaction, neutralization reaction can discharges a large amount of thermal energy, so that bulk temperature increases, can destroy egg
The structure of white matter molecule loses original activity.
(7) 1~2 part 15% of hydrochloric acid will be added by weight in flocculating setting in the product after pH value Secondary Control
Solution while addition and is stirred continuously, and is stirred 2~3 minutes, and test paper comparison, circulate operation, to its pH value are carried out after stirring
It is finely adjusted, pH value is adjusted to 3.5~4.5, natural subsidence 7 hours, removes clear liquid.
(8) it heats, above-mentioned gained sediment is fitted into container, another is put it into and is placed in the container of aqueous solution,
The container for being placed with aqueous solution is heated, is heated to 40 degrees Celsius, 30 minutes is kept the temperature, can ensure that sediment can be same in this way
Step heating, is not in cause protein to lose original activity since local temperature is excessively high in heating process.
(9) it neutralizes, the above-mentioned container equipped with sediment is placed in 10~15 degrees Celsius of temperature of aqueous solution, by weight
Part meter is measured, 1~2 part 30% of sodium hydroxide solution is added into sediment, and is stirred continuously, it is 2~3 minutes exquisite, continue to add
Enter 1~2 part 30% of sodium hydroxide solution and stirring, until adjusting pH value to 6.5~7, obtains the slurries of protein, place
In 10~15 degrees Celsius of aqueous solution and the purpose that is stirred continuously is to improve the speed of heat dissipation.
(10) dry, sediment is dried using spray dryer, the temperature control of air inlet 180~
200 degrees Celsius, the slurries of protein are stirred while spraying, improve the speed of moisture evaporation, the temperature of air outlet is
80 degrees Celsius, to moisture evaporating completely, obtain dry protein raw material.
In the description of this specification, the description meaning of reference term " one embodiment ", " example ", " specific example " etc.
Refer to that particular features, structures, materials, or characteristics described in conjunction with this embodiment or example are contained at least one reality of the invention
It applies in example or example.In the present specification, schematic expression of the above terms are not necessarily referring to identical embodiment or show
Example.Moreover, particular features, structures, materials, or characteristics described can be in any one or more of the embodiments or examples
It can be combined in any suitable manner.
The basic principles, main features and advantages of the present invention have been shown and described above.The technology of the industry
Personnel are it should be appreciated that the present invention is not limited to the above embodiments, and the above embodiments and description only describe this
The principle of invention, without departing from the spirit and scope of the present invention, various changes and improvements may be made to the invention, these
Changes and improvements all fall within the protetion scope of the claimed invention.
Claims (4)
1. the extracting method of protein in a kind of frozen meat thawing water, which comprises the following steps:
(1) first separation takes 8~10 parts of frozen meat thawing waters, passes through the ultrafiltration membrane of 1500 dalton of interception by weight
Hyperfiltration treatment is carried out, the macromolecular substances such as protein therein are retained down from thawing water, collects retention ingredient；
(2) it cleans, retain ingredient and the distilled water after first separation is mixed with the ratio of 1:1, is added and steams in retention ingredient
While distilled water, be added while stirring, and stir it is rectangular always consistent, after water to be distilled is added completely into, continue stirring 4~
5 minutes；
(3) secondary separation carries out the retention ingredient after above-mentioned cleaning at ultrafiltration by the super worry film of 2000 dalton of interception
Reason, the first separation trapped substance after cleaning is retained down from distilled water, collects retention ingredient；
(4) pH value Primary regulation will be added 30% sodium hydroxide solution, while be stirred with glass bar in the ingredient of above-mentioned retention
It mixes, test paper comparison is carried out after stirring, adjust pH value to 6.5~7.5；
(5) enzymatic hydrolysis, the trapped substance by grind slurries quality than albumen complex enzyme and secondary separation for 0.35% is with the ratio of 1:1
It is mixed, temperature is maintained at 50~55 degrees Celsius, and pH value is 7~8, hydrolyzes 3.5 hours；
(6) by weight 1~2 part 30% of hydrochloric acid solution will be added in pH value Secondary Control in the product after above-mentioned hydrolysis,
It is stirred using glass bar, static 4~5 minutes after stirring, test paper comparison is carried out after stirring, continuously adds 1~2 part 30% of hydrochloric acid
Solution is stirred using glass bar, is mixed within static 4~5 minutes after stirring, circulate operation, until adjusting pH value to 4~4.5；
(7) by weight 1~2 part 15% of hydrochloric acid solution will be added in flocculating setting in the product after pH value Secondary Control,
It while addition and is stirred continuously, stirs 2~3 minutes, test paper comparison is carried out after stirring, circulate operation carries out its pH value micro-
It adjusts, pH value is adjusted to 3.5~4.5, natural subsidence 7 hours, removes clear liquid；
(8) it heats, above-mentioned gained sediment is fitted into container, another is put it into and is placed in the container of aqueous solution, to putting
There is the container of aqueous solution to be heated, be heated to 40 degrees Celsius, keeps the temperature 30 minutes；
(9) it neutralizes, the above-mentioned container equipped with sediment is placed in 10~15 degrees Celsius of temperature of aqueous solution, by weight
1~2 part 30% of sodium hydroxide solution is added into sediment, and is stirred continuously for meter, 2~3 minutes exquisite, continuously add 1~
2 part 30% of sodium hydroxide solution and stirring obtain the slurries of protein until adjusting pH value to 6.5~7；
(10) dry, sediment is dried using spray dryer, the temperature control of air inlet is taken the photograph 180~200
Family name's degree is stirred the slurries of protein while spraying, improves the speed of moisture evaporation, and the temperature of air outlet is 80 Celsius
Degree, to moisture evaporating completely, obtains dry protein raw material.
2. the extracting method of protein in a kind of frozen meat thawing water according to claim 1, which is characterized in that described one
In secondary separation, in 0.15~0.25 Pascal, temperature is 28~32 degrees Celsius for the intake pressure control of ultrafiltration membrane.
3. the extracting method of protein in a kind of frozen meat thawing water according to claim 1, which is characterized in that described two
In secondary separation, in 0.12~0.20 Pascal, temperature is 28~32 degrees Celsius for the intake pressure control of ultrafiltration membrane.
4. the extracting method of protein in a kind of frozen meat thawing water according to claim 1, which is characterized in that the egg
White complex enzyme includes alkali protease, lipase and trypsase.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811007780.7A CN109207540A (en) | 2018-08-31 | 2018-08-31 | The extracting method of protein in a kind of frozen meat thawing water |
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| CN201811007780.7A CN109207540A (en) | 2018-08-31 | 2018-08-31 | The extracting method of protein in a kind of frozen meat thawing water |
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| CN109207540A true CN109207540A (en) | 2019-01-15 |
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| CN201811007780.7A Pending CN109207540A (en) | 2018-08-31 | 2018-08-31 | The extracting method of protein in a kind of frozen meat thawing water |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107674897A (en) * | 2016-09-30 | 2018-02-09 | 青岛大学 | One kind is by method for extracting proteins in bata-carotene zymotic fluid |
| CN107674898A (en) * | 2016-09-30 | 2018-02-09 | 青岛大学 | One kind is by method for extracting proteins in farnoquinone zymotic fluid |
| CN107674899A (en) * | 2016-09-30 | 2018-02-09 | 青岛大学 | One kind is by method for extracting proteins in vitamin B12 zymotic fluid |
-
2018
- 2018-08-31 CN CN201811007780.7A patent/CN109207540A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107674897A (en) * | 2016-09-30 | 2018-02-09 | 青岛大学 | One kind is by method for extracting proteins in bata-carotene zymotic fluid |
| CN107674898A (en) * | 2016-09-30 | 2018-02-09 | 青岛大学 | One kind is by method for extracting proteins in farnoquinone zymotic fluid |
| CN107674899A (en) * | 2016-09-30 | 2018-02-09 | 青岛大学 | One kind is by method for extracting proteins in vitamin B12 zymotic fluid |
Non-Patent Citations (1)
| Title |
|---|
| 王晓凡等: "菜籽蛋白提取及应用研究进展", 《粮食与油脂》 * |
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Application publication date: 20190115 |