CN107674899A - One kind is by method for extracting proteins in vitamin B12 zymotic fluid - Google Patents
One kind is by method for extracting proteins in vitamin B12 zymotic fluid Download PDFInfo
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- CN107674899A CN107674899A CN201610876802.8A CN201610876802A CN107674899A CN 107674899 A CN107674899 A CN 107674899A CN 201610876802 A CN201610876802 A CN 201610876802A CN 107674899 A CN107674899 A CN 107674899A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/34—Extraction; Separation; Purification by filtration, ultrafiltration or reverse osmosis
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Abstract
The present invention produces the protein of low molecule amount using vitamin B12 fermented waste fluid as raw material, has both reduced the waste to resource, while solve the problems, such as that zymotic fluid pollutes to environment, has a good application prospect well again.Meanwhile cost of material of the present invention is low, equipment is simple, easy to operate, it is easy to accomplish large-scale production, improves industry added value.
Description
Technical field
The present invention relates to one kind by method for extracting proteins in vitamin B12 zymotic fluid, more particularly to mycelial receipts
The broken production technology with Separation of Proteins extraction of collection.
Background technology
Vitamin B12 is called cobalamin, is the unique vitamin containing metallic element.Vitamin B12 in nature is all
Microbe synthesis, high animals and plants can not manufacture vitamin B12.Vitamin B12 is a kind of a kind of unique enteron aisle point of needs
Secretion (castle's intrinsic factor) helps could absorbed vitamin.Somebody lacks this castle's intrinsic factor because stomach is abnormal, even if
Source abundance can also suffer from pernicious anaemia in meals.Vitamin B12 is there is no in plant food.When it is stopped in enteron aisle
Between it is long, taking around three hours (most of water soluble vitamins only need several seconds) could be absorbed.Vitamin B12 it is main
Physiological function is to participate in manufacture erythrocyte, prevents pernicious anaemia；Prevent cerebral nerve from being destroyed.Vitamin B12 is B races
One kind the latest is found in vitamin so far.Vitamin B12 is a kind of polycyclic based compound containing trivalent cobalt, and 4 reduce
Pyrrole ring connect together and be turned into 1 big ring of corrin (similar to porphyrin), be the core of vitamin B12 molecule.So contain this
The compound of kind ring is all referred to as corrinoid.Vitamin B12 be light red acicular crystal, soluble in water and ethanol, in pH value
It is most stable under 4.5-5.0 mild acid conditions, strong acid (pH<2) or in alkaline solution decompose, heat can have to be destroyed to a certain degree, but short
The high-temperature sterilization loss of time is small, and it is easily destroyed to meet strong light or ultraviolet.Common gastronomical process loss amount about 30%.
The physiological function of vitamin B12 mainly has two：1. as the co-factor of transmethylase, methionine, chest are participated in
The synthesis of gland pyrimidine etc., methyl tetrahydrofolate is such as set to be changed into tetrahydrofolic acid and methyl is transferred into acceptors (such as homotype half
Cystine), acceptors is turned into methyl-derivatives (such as methionine i.e. methyl homocysteine).Therefore vitamin B12
It can promote the biosynthesis of protein, influence growing for infant during shortage.2. protect folic acid transfer in the cell and
Storage.During vitamin B12 deficiency, human erythrocyte's folate content is low, and the folic acid of liver storage reduces, and this may be with vitamin
B12 lacks, and causes methyl to be shifted from homocysteine to methionine difficult relevant, methyl is assembled in the cell, compromises
The storage of tetrahydrofolic acid in the cell, because the tendency that tetrahydrofolic acid is combined into methyl tetrahydrofolate with methyl is strong, the latter's synthesis
Polyglutamic acid.
Because vitamin B12 is complicated, chemical synthesis process is cumbersome and costly.It is initially by from Animal Liver
Dirty wait is extracted in tissue, but efficiency is low.Extracted later from streptomycin fermentation waste liquid, but production capacity stage efficiency still extremely has
Limit.Commercially produced until in the 1970s, starting special microorganism, the production capacity of global vitamin B12 just steps up.
It is relatively low that the yield of vitamin B12 is produced by anaerobic fermentation, domestic each manufacturing enterprise is substantially carried out aerobic fermentation.Currently, it is domestic
The fermentation level of oxygen consumption Production by Microorganism Fermentation vitamin B12 has approached in 200-260mg/L, good Batch fermentation level
300mg/L。
The bacterial strain of aerobic fermentation mainly takes off single pseudomonad (P.denitrificans), and culture medium is with beet molasses, wheat
Bud sugar, sucrose are carbon source, using corn steep liquor and yeast extract as nitrogen source, add inorganic salts and Co, and add 5,6- dimethylbiphenyl miaows
Azoles and glycine betaine are as precursor.The aerobic propionibacterium freudenreichii of strain (P.freudenreichii) of anaerobic ferment process use,
Xie Shi Propionibacteriums (P.shermanii).Propionibacterium degraded glucose produces the Wood-Wo Keman reactions way of propionic acid, acetic acid
Be related to methylmalonyl-coenzyme a mutase in footpath, the enzyme needs vitamin B12 as coenzyme, so in Propionibacterium there is
The a full set of inhereditary material and complete route of synthesis of biosynthesis vitamin B12.
Vitamin B12 can produce substantial amounts of mycelium, albumen after using Xie Shi Propionibacteriums (P.shermanii) fermentation
The accessory substances such as matter, ammoniacal nitrogen, viscosity is bigger if ultrafiltration retains composition, waste of resource and pollution ring if directly giving up
Border.In order to effectively utilize resource, environmental protection, it is necessary to the fermented waste fluid of vitamin B12 is handled, extraction wherein has
With material, and reduce its destruction to environment.In this sense, protein is extracted from vitamin B12 zymotic fluid to be used as
Forage protein is significant.
The technology that Wang Jinyu etc. have studied the recovery propionic acid from vitamin B12 fermented waste fluid (uses complexing abstraction from dimension
Propionic acid, chemical industry environmental protection, 05 phase in 2004 are reclaimed in raw plain B12 fermented waste fluids).But currently without in vitamin B12 zymotic fluid
Protein Recovery technology expansion research.
The content of the invention
For the deficiencies in the prior art, albumen is extracted from vitamin B12 zymotic fluid the invention provides one kind
The method of matter, this method quickly and easily can extract protein from waste liquid, can further make the protein of extraction
To produce the raw material of feed.The technology of the present invention had both reduced the waste to resource, while solved zymotic fluid pair well again
The problem of environment pollutes, have a good application prospect.
The invention provides one kind, method for extracting proteins, step are as follows from vitamin B12 zymotic fluid：
(1) vitamin B12 zymotic fluid is subjected to hyperfiltration treatment with milipore filter, mycelium therein and protein etc. is divided greatly
Sub- material is retained from waste liquid and come out, and collects retention composition；The molecular cut off of the milipore filter is 3000 dalton, influent pressure
Power 0.7-0.8Mpa, 20 DEG C of ultrafiltrate temperature, zymotic fluid pH value 5.0-6.0.
(2) the retention composition obtained by step (1) ultrafiltration is added into water to stir, then will using super low temperature quick frozen technology
It is quick-frozen to retain ingredient solution, obtains suspension, gained suspension is then subjected to break process with high pressure homogenizer again；The addition of water
Volume and the volume ratio of retention composition are 1:2.5-1:5；Super low temperature quick frozen temperature is -40--50 DEG C, and the quick-frozen time is 8min；It is outstanding
The pressure that turbid passes through high pressure homogenizer is 180Mpa, flow velocity 120m/s.
(3) it is 6 the pH of the suspension of step (2) to be adjusted into pH, adds albumen complex enzyme A, enzymolysis and extraction albumen therein
Matter composition；The albumen complex enzyme A is made up of papain, bromelain, acid protease, consisting of Papain
Enzyme：Bromelain：The mass ratio of acid protease is 1：1.1-1.3：0.8-1.2, hydrolysis temperature are 30 DEG C, the dosage of enzyme
For the 1.5%-2% of suspension quality, enzymolysis time 4h.
(4) liquid after being hydrolyzed in step (3) is subjected to hyperfiltration treatment with milipore filter again, collects retention composition；It is described
The molecular cut off of milipore filter is 1000 dalton, intake pressure 1.0-1.1Mpa, 20 DEG C of ultrafiltrate temperature.
(5) stirred after the retention composition of step (4) being added into water, the addition volume and the volume ratio of retention composition of water
For 1:3-4, regulation pH are 6.8-7.8, add albumen complex enzyme B, further digest protein component therein, the albumen
Complex enzyme B is made up of pancreatin, neutral proteinase, elastoser, consisting of pancreatin：Neutral proteinase：Elastoser
Mass ratio is 1：1.4-1.6：1.2-1.5, hydrolysis temperature are 35 DEG C, and the dosage of enzyme is the 1.0%-1.2% of suspension quality,
Enzymolysis time is 2h.
(6) enzymolysis liquid for digesting gained is centrifuged, collects supernatant, centrifuge and carried out at 35 DEG C, rotating speed is
2000rpm, time 2h.
(7) pH to 3.0-4.0 of regulating step (6) described supernatant, makes protein flocculating setting, sedimentation time 10h,
Filtering, precipitation is collected, it is drying precipitated, albumen powder is obtained, dries the method using spray drying, spray drying EAT is
180 DEG C, leaving air temp is 100 DEG C.
The present invention has the advantage that and good effect：
The present invention produces the protein of low molecule amount using vitamin B12 fermented waste fluid as raw material, has both reduced to resource
Waste, while solve the problems, such as that zymotic fluid pollutes to environment, has a good application prospect well again.Meanwhile this
Invention the compositions such as vitamin B12 fermentation mycelium have been carried out using homogenizer it is first it is quick-frozen after crush, and employ two times of ultrafiltration
With the mode of enzymolysis, the protein in waste liquid is extracted using the compound protease with specific proportioning and composition, egg
The recovery rate of white matter is high, and molecular weight is small, will be easier to absorb for animal with the feed of this protein production.In addition, raw material of the present invention
Cost is low, and equipment is simple, easy to operate, it is easy to accomplish large-scale production, reduces pollution of the waste liquid to environment, improves industry
Added value.
Embodiment
According to following embodiments, the present invention may be better understood.It is however, as it will be easily appreciated by one skilled in the art that real
Apply concrete technology condition, material proportion and its result described by example and be merely to illustrate the present invention, without should also without limitation on
The present invention described in claims.
In following embodiments, pH is adjusted using 30wt% hydrochloric acid solution, 30wt% sodium hydroxide solution.
Embodiment 1
(1) vitamin B12 zymotic fluid 500g is taken, hyperfiltration treatment is carried out with milipore filter, by mycelium therein and protein
Retain and come out from waste liquid Deng macromolecular substances, collect retention composition；The molecular cut off of the milipore filter is 3000 dalton,
Intake pressure 0.7Mpa, 20 DEG C of ultrafiltrate temperature, zymotic fluid pH value 6.0.
(2) the retention composition obtained by step (1) ultrafiltration is added into water to stir, then will using super low temperature quick frozen technology
It is quick-frozen to retain ingredient solution, obtains suspension, gained suspension is then subjected to break process with high pressure homogenizer again；The addition of water
Volume and the volume ratio of retention composition are 1:2.5；Super low temperature quick frozen temperature is -40 DEG C, and the quick-frozen time is 8min；Suspension passes through
The pressure of high pressure homogenizer is 180Mpa, flow velocity 120m/s.
(3) it is 6 the pH of the suspension of step (2) to be adjusted into pH, adds albumen complex enzyme A, enzymolysis and extraction albumen therein
Matter composition；The albumen complex enzyme A is made up of papain, bromelain, acid protease, consisting of Papain
Enzyme：Bromelain：The mass ratio of acid protease is 1：1.3：0.8, hydrolysis temperature is 30 DEG C, and the dosage of enzyme is suspension
The 1.5% of quality, enzymolysis time 4h.
(4) liquid after being hydrolyzed in step (3) is subjected to hyperfiltration treatment with milipore filter again, collects retention composition；It is described
The molecular cut off of milipore filter is 1000 dalton, intake pressure 1.0Mpa, 20 DEG C of ultrafiltrate temperature.
(5) stirred after the retention composition of step (4) being added into water, the addition volume and the volume ratio of retention composition of water
For 1:3, regulation pH is 6., adds albumen complex enzyme B, further digests protein component therein, the albumen complex enzyme B
It is made up of pancreatin, neutral proteinase, elastoser, consisting of pancreatin：Neutral proteinase：The mass ratio of elastoser
For 1：1.4：1.5, hydrolysis temperature is 35 DEG C, and the dosage of enzyme is the 1.0% of suspension quality, enzymolysis time 2h.
(6) enzymolysis liquid for digesting gained is centrifuged, collects supernatant, centrifuge and carried out at 35 DEG C, rotating speed is
2000rpm, time 2h.
(7) pH to 4.0 of regulating step (6) described supernatant, makes protein flocculating setting, sedimentation time 10h, mistake
Filter, precipitation is collected, it is drying precipitated, albumen powder 206.5g is obtained, dries the method using spray drying, spray drying EAT
For 180 DEG C, leaving air temp is 100 DEG C.
Embodiment 2
(1) vitamin B12 zymotic fluid 500g is taken, hyperfiltration treatment is carried out with milipore filter, by mycelium therein and protein
Retain and come out from waste liquid Deng macromolecular substances, collect retention composition；The molecular cut off of the milipore filter is 3000 dalton,
Intake pressure 0.8Mpa, 20 DEG C of ultrafiltrate temperature, zymotic fluid pH value 5.0.
(2) the retention composition obtained by step (1) ultrafiltration is added into water to stir, then will using super low temperature quick frozen technology
It is quick-frozen to retain ingredient solution, obtains suspension, gained suspension is then subjected to break process with high pressure homogenizer again；The addition of water
Volume and the volume ratio of retention composition are 1:5；Super low temperature quick frozen temperature is -50 DEG C, and the quick-frozen time is 8min；Suspension passes through height
The pressure for pressing homogenizer is 180Mpa, flow velocity 120m/s.
(3) it is 6 the pH of the suspension of step (2) to be adjusted into pH, adds albumen complex enzyme A, enzymolysis and extraction albumen therein
Matter composition；The albumen complex enzyme A is made up of papain, bromelain, acid protease, consisting of Papain
Enzyme：Bromelain：The mass ratio of acid protease is 1：1.1：1.2, hydrolysis temperature is 30 DEG C, and the dosage of enzyme is suspension
The 2% of quality, enzymolysis time 4h.
(4) liquid after being hydrolyzed in step (3) is subjected to hyperfiltration treatment with milipore filter again, collects retention composition；It is described
The molecular cut off of milipore filter is 1000 dalton, intake pressure 1.1Mpa, 20 DEG C of ultrafiltrate temperature.
(5) stirred after the retention composition of step (4) being added into water, the addition volume and the volume ratio of retention composition of water
For 1:4, pH7.8 is adjusted, albumen complex enzyme B is added, further digests protein component therein, the albumen complex enzyme B
It is made up of pancreatin, neutral proteinase, elastoser, consisting of pancreatin：Neutral proteinase：The mass ratio of elastoser
For 1：1.6：1.2, hydrolysis temperature is 35 DEG C, and the dosage of enzyme is suspension quality 1.2%, enzymolysis time 2h.
(6) enzymolysis liquid for digesting gained is centrifuged, collects supernatant, centrifuge and carried out at 35 DEG C, rotating speed is
2000rpm, time 2h.
(7) pH to 3. of regulating step (6) described supernatant, make protein flocculating setting, sedimentation time 10h, filter,
Precipitation is collected, it is drying precipitated, albumen powder 197.8g is obtained, dries the method using spray drying, spray drying EAT is
180 DEG C, leaving air temp is 100 DEG C.
Embodiment 3
The quality testing of the protein reclaimed in embodiment 1 or 2, detect the Mass Distribution of protein in regenerant, knot
Fruit is shown in Table 1：
Table 1 reclaims the molecular weight distribution of protein
| Molecular weight (dalton) | Embodiment 1 (%) | Embodiment 2 (%) |
| More than 5000 | 1.2 | 1.9 |
| 4000-5000 | 5.4 | 3.7 |
| 3000-4000 | 6.3 | 5.2 |
| 2000-3000 | 8.9 | 7.4 |
| 1000-2000 | 78.2 | 81.8 |
The protein obtained by extracting method of the present invention is used it can be seen from the result of above-mentioned quality testing
In, molecular weight be in the protein of 1000-2000 dalton content be more than 75%, by described protein be used as feed it
Afterwards, easily absorbed by animal, there is good effect.
Claims (3)
1. one kind method for extracting proteins from vitamin B12 zymotic fluid, it is characterised in that：This method is sent out with vitamin B12
Ferment waste liquid is raw material, the compositions such as vitamin B12 fermentation mycelium have been carried out it is first it is quick-frozen after it is broken, and employ two times of ultrafiltration and
The mode of enzymolysis, the protein in waste liquid is extracted using the compound protease with specific proportioning and composition.
2. method for extracting proteins in the zymotic fluid according to claim 1 from vitamin B12, its specific step is such as
Under：
(1) vitamin B12 zymotic fluid is subjected to hyperfiltration treatment with milipore filter, by macromolecular complex such as mycelium therein and protein
Matter is retained from waste liquid and come out, and collects retention composition；The molecular cut off of the milipore filter is 3000 dalton, intake pressure
0.7-0.8Mpa, 20 DEG C of ultrafiltrate temperature, zymotic fluid pH value 5.0-6.0；
(2) the retention composition obtained by step (1) ultrafiltration is added into water to stir, then will be retained using super low temperature quick frozen technology
Ingredient solution is quick-frozen, obtains suspension, and gained suspension then is carried out into break process with high pressure homogenizer again；The addition volume of water
Volume ratio with retaining composition is 1:2.5-1:5；Super low temperature quick frozen temperature is -40--50 DEG C, and the quick-frozen time is 8min；Suspension
Pressure by high pressure homogenizer is 180Mpa, flow velocity 120m/s；
(3) pH of the suspension of step (2) is adjusted to pH as 6, adds albumen complex enzyme A, enzymolysis and extraction protein therein into
Point；The albumen complex enzyme A is made up of papain, bromelain, acid protease, consisting of papain：
Bromelain：The mass ratio of acid protease is 1：1.1-1.3：0.8-1.2, hydrolysis temperature are 30 DEG C, and the dosage of enzyme is
The 1.5%-2% of suspension quality, enzymolysis time 4h；
(4) liquid after being hydrolyzed in step (3) is subjected to hyperfiltration treatment with milipore filter again, collects retention composition；The ultrafiltration
The molecular cut off of film is 1000 dalton, intake pressure 1.0-1.1Mpa, 20 DEG C of ultrafiltrate temperature；
(5) stirred after the retention composition of step (4) being added into water, the addition volume of water and the volume ratio of retention composition are 1:
3-4, regulation pH are 6.8-7.8, add albumen complex enzyme B, further digest protein component therein, and the albumen is compound
Enzyme B is made up of pancreatin, neutral proteinase, elastoser, consisting of pancreatin：Neutral proteinase：The quality of elastoser
Ratio is 1：1.4-1.6：1.2-1.5, hydrolysis temperature are 35 DEG C, and the dosage of enzyme is the 1.0%-1.2% of suspension quality, enzymolysis
Time is 2h；
(6) enzymolysis liquid for digesting gained is centrifuged, collects supernatant, centrifuge and carried out at 35 DEG C, rotating speed is
2000rpm, time 2h；
(7) pH to 3.0-4.0 of regulating step (6) described supernatant, makes protein flocculating setting, sedimentation time 10h, mistake
Filter, precipitation is collected, it is drying precipitated, albumen powder is obtained, dries the method using spray drying, spray drying EAT 180
DEG C, leaving air temp is 100 DEG C.
3. method for extracting proteins in the zymotic fluid according to claim 1 or 2 from vitamin B12, wherein using
30wt% hydrochloric acid solution, 30wt% sodium hydroxide solution adjust pH.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109207540A (en) * | 2018-08-31 | 2019-01-15 | 安徽老炊食品有限公司 | The extracting method of protein in a kind of frozen meat thawing water |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102318724A (en) * | 2011-09-22 | 2012-01-18 | 上海源耀生物科技有限公司 | Production method of acid soybean peptide protein |
| CN102766671A (en) * | 2012-08-09 | 2012-11-07 | 山东天力药业有限公司 | Method for extracting proteins from vitamin C fermented mash |
-
2016
- 2016-09-30 CN CN201610876802.8A patent/CN107674899A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102318724A (en) * | 2011-09-22 | 2012-01-18 | 上海源耀生物科技有限公司 | Production method of acid soybean peptide protein |
| CN102766671A (en) * | 2012-08-09 | 2012-11-07 | 山东天力药业有限公司 | Method for extracting proteins from vitamin C fermented mash |
Non-Patent Citations (2)
| Title |
|---|
| 《化工百科全书》编辑委员会编: "《化工百科全书 第11卷》", 31 August 1996 * |
| 张建民等: "超滤膜分离技术在维生素B12生产中的应用", 《河北化工》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109207540A (en) * | 2018-08-31 | 2019-01-15 | 安徽老炊食品有限公司 | The extracting method of protein in a kind of frozen meat thawing water |
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Application publication date: 20180209 |