CN109206475A - A method of it is digested using single step while extracting oil body and albumen - Google Patents

A method of it is digested using single step while extracting oil body and albumen Download PDF

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Publication number
CN109206475A
CN109206475A CN201811081288.4A CN201811081288A CN109206475A CN 109206475 A CN109206475 A CN 109206475A CN 201811081288 A CN201811081288 A CN 201811081288A CN 109206475 A CN109206475 A CN 109206475A
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oil body
extracting method
enzyme
soybean
hydrolase
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CN109206475B (en
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齐宝坤
李杨
孙禹凡
韩璐
钟明明
胡淼
张爽
谢凤英
曹亮
赵城彬
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Northeast Agricultural University
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Northeast Agricultural University
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
    • C11B1/00—Production of fats or fatty oils from raw materials
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
    • C11B1/00—Production of fats or fatty oils from raw materials
    • C11B1/02—Pretreatment
    • C11B1/04—Pretreatment of vegetable raw material

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Fats And Perfumes (AREA)
  • Edible Oils And Fats (AREA)

Abstract

A method of it is digested using single step while extracting oil body and albumen, belong to biotechnology applications technical field.The present invention solves existing method the technical issues of there are complex steps, and extraction time is long, the yield of oil body.Method: soybean is cleared up, decortication is impregnated, is sterilized after crushing, adjust water content, bacterial strain, fermentation to producing enzyme, centrifugation are accessed into solid state substrate, supernatant is collected as hydrolase, and be added in the mixed liquor of soy bean powder and water and obtain enzymolysis liquid, it is ultrasonically treated after enzyme deactivation, is centrifugally separating to obtain oil body and protein.The present invention for the first time combines solid state fermentation with ultrasonic technique, the synchronous effective component extracted in soybean, the present invention is compared with tradition extracts the method for oil body, the enzyme and ultrasonic treatment that aspergillus niger generates adequately destroy cell wall structure, release complete oil body and protein, required equipment is simple, recovery rate is high, and the product of acquisition can be widely applied to the industries such as food, change product and medicine.

Description

A method of it is digested using single step while extracting oil body and albumen
Technical field
The invention belongs to the application field of biotechnology in the food industry, and in particular to a kind of to be digested simultaneously using single step The method for extracting oil body and protein.
Background technique
Oil body is the organelle for being used to store grease in vegetable seeds, provides energy for growth after germination.Its diameter Usually 0.5~2.5um, the basic structure of oil body are that the liquid matrix inside grease body is triglycerides, and surface is by single layer phosphorus The biomembrane covering of rouge-endogenous protein composition, therefore oil body is a kind of natural microcapsules, and can form stable water Packet fat liquor.Soybean oil body is rich in the functional components such as polyunsaturated fatty acid, phosphatide, tocopherol and phytosterol, therefore complete Whole oil body can provide very high commercial value, have huge application potential in food.
The extracting method of oil body constantly improves at present.Nineteen ninety Jacks etc. impregnated seed, defibrination, filtering, It is centrifuged and washs 14 times, be finally recovered out oil body；Tzen etc. proposes a kind of classical way that Plant seed oil-bodies extract: seed It impregnates (peanut, soybean and sunflower seeds first impregnate 6h) or does not impregnate (rape and sesame), with the phosphate of the sucrose containing 0.6mol/L Buffer defibrination, centrifugation, and repeatedly washed with Tween-20, sodium chloride, urea, complete, high-purity oil body can be extracted； Chen etc. reports a kind of simple soybean oil body extracting method, and key step includes adjusting raw soya-bean milk pH 11, and centrifugation obtains thick Oil body, then crude oil body is washed twice at pH 11.Since the step of extracting oil body is cumbersome, extraction time is long, while cumbersome Extraction step can also be such that oil body is lost, and reduce grease body extraction efficiency, affect the yield of grease body；And use multi-solvents The conventional method of oil body is extracted from oily seed, solvent can remain in grease body surface face and ring oil body quality, therefore limit Comprehensive utilization in food, cosmetics and pharmaceuticals industry.
Summary of the invention
The present invention solves existing extract oil body method, and there are complex steps, and extraction time is long, and the technology of the yield of oil body is asked Topic provides a kind of method for digesting using single step while extracting oil body and albumen.The method of the present invention adequately destroys cell wall Structure, and release complete oil body and protein.The method of the present invention simplifies the extracting method of soybean oil body, with to greatest extent Complete oil body and protein are collected to realize higher value application in ground.
The technical problem to be solved by the present invention is to what is be achieved through the following technical solutions:
Method includes the following steps:
Step 1: high-temperature sterilization after soybean is successively cleared up, is peeled, is impregnated, is crushed, adjusts water content；
Step 2: then access aspergillus niger strain (logarithmic phase bacterium solution or spore suspension) is fermented to producing enzyme, centrifugation, Supernatant is collected as hydrolase；
Step 3: expanded soybean is crushed, water is added, adjusts pH value, hydrolase, heat preservation enzymatic hydrolysis are added under the conditions of 50 DEG C；
Step 4: then enzyme deactivation, is being ultrasonically treated, is being centrifugally separating to obtain oil body and protein.
It further limits, high-temperature sterilization temperature described in step 1 is 121 DEG C, time 15min.
It further limits, water content is adjusted in step 1 to the 20% of its dry weight；Inoculum concentration is object in step 2 Expect the 15% of dry weight；The condition of the fermentation are as follows: relative humidity 70%, temperature are 25 DEG C, time 48h, plain enzyme activity Power reaches 0.68FPU/mL or more, and Xylanase activity reaches 95U/mL or more, and pectinase activity reaches 5.5U/mL or more, egg White enzyme activity reaches 65.9U/mL or more, and alpha-galactoside enzyme activity reaches 8.4U/mL or more.
It further limits, expanded soybean is mixed with water according to the weight ratio of 1:3 after crushing in step 3.
It further limits, pH value is 4.8 (adjust and arrive enzyme optimum pH) in step 3.
It further limits, adds hydrolase by 1mL/g-10mL/g concentration in step 3；It is preferred that: add by 2mL/g concentration Add hydrolase.
It further limits, ultrasonic power described in step 4 is 30w-200w, ultrasonic time 5min-15min；It is preferred that: Ultrasonic power is 120w, ultrasonic time 10min.
The present invention for the first time combines solid state fermentation with ultrasonic technique, the synchronous effective component extracted in soybean, the present invention Compared with tradition extracts the method for oil body, the enzyme and ultrasonic treatment that aspergillus niger generates can adequately destroy cell wall structure, have Conducive to the release of intracellular effective component, complete oil body and protein are released, required equipment is simple, safe operation, extraction Rate is high, and the product of acquisition can be widely applied to the industries such as food, change product and medicine；Oil body recovery rate is up to 98%, protein Recovery rate is up to 90%.
The present invention can carry out continuous production multiple product, have significant economic benefit, environmental benefit and social benefit.
Specific embodiment
Specific embodiment 1: a kind of in present embodiment digested while the method for extracting oil body and albumen is using single step It carries out in the steps below:
Step 1: soybean is successively cleared up, peel, impregnates, be put in high-pressure sterilizing pot after crushing, in 121 DEG C of items High-temperature sterilization 15min under part adjusts moisture content of material to the 20% of its material dry weight；
Step 2: then aspergillus niger strain (logarithmic phase bacterium solution) is accessed, in relative humidity 70%, the condition that temperature is 25 DEG C Lower fermented and cultured 48h, centrifugation collect supernatant as hydrolase；
Step 3: expanded soybean is crushed, water is added, water is added by the concentration of 10mL/g under the conditions of 4.8,50 DEG C of pH value Solve enzyme, heat preservation enzymatic hydrolysis 6h；
Step 4: then enzyme deactivation, is ultrasonically treated 10min under the conditions of ultrasonic power is 120w, is centrifugally separating to obtain oil body And protein；
Wherein, the inoculum concentration of step 2 is the 15% of material dry weight；Under conditions of fermentation liquid is with 9000g, centrifugation 10 Minute, it is mixed with water according to the weight ratio of 1:3 after expanded soybean is crushed.
Plain enzyme activity reaches 0.68FPU/mL or more in supernatant, and Xylanase activity reaches 95U/mL or more, pectase Vigor reaches 5.5U/mL or more, and prolease activity reaches 65.9U/mL or more, alpha-galactoside enzyme activity reach 8.4U/mL with On.
The oil body recovery rate of present embodiment method is up to 95%, and extraction rate of protein is up to 63%.
Comparative example 1: with specific implementation method one the difference lies in that in step 4, ultrasonic power 0w, ultrasonic time is 0min.Other steps and parameter are identical as specific implementation method one.
The oil body recovery rate of 1 method of comparative example is up to 84%, and extraction rate of protein is up to 63%.
Comparative example 2: with specific implementation method one the difference lies in that in step 4, the concentration for adding hydrolase is 0mL/g. Other steps and parameter are identical as specific implementation method one.
The oil body recovery rate of 2 method of comparative example is up to 23%, and extraction rate of protein is up to 38%.

Claims (10)

1. a kind of method for being digested using single step while extracting oil body and albumen, it is characterised in that the extracting method is by following What step carried out:
Step 1: high-temperature sterilization after soybean is successively cleared up, is peeled, is impregnated, is crushed, adjusts water content；
Step 2: then access aspergillus niger strain ferments to producing enzyme, centrifugation, supernatant is collected as hydrolase；
Step 3: expanded soybean is crushed, water is added, adjusts pH value, hydrolase, heat preservation enzymatic hydrolysis are added under the conditions of 50 DEG C；
Step 4: then enzyme deactivation, is being ultrasonically treated, is being centrifugally separating to obtain oil body and protein.
2. extracting method according to claim 1, it is characterised in that high-temperature sterilization temperature described in step 1 is 121 DEG C, the time For 15min.
3. extracting method according to claim 1, it is characterised in that adjust water content in step 1 to its dry weight 20%.
4. extracting method according to claim 1, it is characterised in that inoculum concentration is the 15% of material dry weight in step 2.
5. extracting method according to claim 1, it is characterised in that the condition of fermentation described in step 2 are as follows: relative humidity is 70%, temperature is 25 DEG C, time 48h.
6. extracting method according to claim 1, it is characterised in that with water according to 1:3's after expanded soybean crushes in step 3 Weight ratio mixing；PH value is 4.8.
7. extracting method according to claim 1, it is characterised in that by the addition hydrolysis of 1mL/g-10mL/g concentration in step 3 Enzyme.
8. extracting method according to claim 1, it is characterised in that add hydrolase by 2mL/g concentration in step 3.
9. extracting method according to claim 1, it is characterised in that ultrasonic power described in step 4 is 30w-200w, when ultrasonic Between be 5min-15min.
10. extracting method according to claim 1, it is characterised in that ultrasonic power described in step 4 is 120w, ultrasonic time For 10min.
CN201811081288.4A 2018-09-17 2018-09-17 Method for simultaneously extracting oil body and protein by single-step enzymolysis Active CN109206475B (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115067501A (en) * 2022-06-21 2022-09-20 东北农业大学 Preparation method of stable Pickering emulsion containing soybean protein

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115067501A (en) * 2022-06-21 2022-09-20 东北农业大学 Preparation method of stable Pickering emulsion containing soybean protein

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