CN109188007B - Reaction disc of full-automatic biochemical analyzer and use method thereof - Google Patents
Reaction disc of full-automatic biochemical analyzer and use method thereof Download PDFInfo
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- CN109188007B CN109188007B CN201811035109.3A CN201811035109A CN109188007B CN 109188007 B CN109188007 B CN 109188007B CN 201811035109 A CN201811035109 A CN 201811035109A CN 109188007 B CN109188007 B CN 109188007B
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
- G01N35/04—Details of the conveyor system
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
- G01N35/04—Details of the conveyor system
- G01N2035/0401—Sample carriers, cuvettes or reaction vessels
Abstract
The invention relates to a reaction disc of a full-automatic biochemical analyzer and a using method thereof.A reaction disc cover is provided with fixed and movable cup groove components which are distributed in central symmetry. The fixed cup groove component is fixed at the outer edge of the reaction disk cover. The movable bracket of the movable cup groove component is movably connected with the outer edge of the reaction disc cover through a rotating shaft and a rotating shaft seat. When the reaction tray cover assembly rotates at high speed, the movable bracket and the bottom of the cup groove of the movable bracket are turned upwards and outwards from the reaction tray, and the intersection angle of the movable bracket and the horizontal plane reaches 120 degrees. The application method comprises the steps of firstly adding a liquid sample to be centrifuged into a cup slot of the movable bracket, wherein the sample adding amount ensures that the cup slot of the movable bracket is centrosymmetric; the reaction disc cover assembly rotates at a high speed, and the sample liquid in the reaction cup is centrifuged; distributing the clear liquid into a reaction cup of the fixed cup groove; and testing according to a conventional method. The reaction tray cover assembly and the centrifugal machine are combined into a whole, sample liquid does not need to be processed additionally, the testing efficiency is improved, and real full-automatic biochemical analysis is realized.
Description
Technical Field
The invention relates to a full-automatic biochemical analyzer technology, in particular to a reaction disc of a full-automatic biochemical analyzer and a using method thereof.
Background
The current common biochemical detection methods are as follows: colorimetry, electrophoresis, immunoelectrophoresis, immunoturbidimetric emission immunoassay, enzyme-linked immunosorbent assay, etc., and the most common method is immunoturbidimetric assay. Immunoturbidimetry is further classified into immunotransmission turbidimetry and immunodispersion turbidimetry. Most biochemical analyzers in the market at present adopt an immunoturbidimetry method to perform qualitative and quantitative analysis according to absorption spectra of substances.
The reaction disc assembly is one of the core parts of the biochemical analyzer, is used for bearing the reaction cup, transmitting the reaction cup to the photoelectric data acquisition position, enabling the sample and the reagent to react in the thermostatic bath, and measuring the absorbance of the reaction solution.
The biochemical analyzers for measuring biochemical projects by an immunoturbidimetry method in the market at present have two types, one is an external centrifugation mode, liquid to be measured is placed in a centrifugal tube and then placed on a centrifugal machine for centrifugation, and after the centrifugation is finished, sample clear liquid after the centrifugation is placed on a conventional biochemical analyzer for detection. In another mode, an ultrafiltration membrane is adopted to filter a liquid sample, and then the filtered sample clear liquid is put on a conventional biochemical analyzer for detection.
The external centrifugation treatment of the original liquid sample needs to manually transfer the centrifuged sample clear liquid to a biochemical analyzer, the process needs to be very careful, and if the container collides or shakes during moving and placing, the centrifuged and precipitated sample clear liquid becomes muddy again, so that the test result is influenced. The ultrafiltration membrane is adopted to filter the liquid sample, so that the filtering step is added, and no matter manual filtration or instrument filtration is adopted, new consumables are required to be introduced, so that the detection cost is increased; particularly, the filtering process has pollution risk, and the visible components are easy to mix into the sample liquid to influence the test result.
In a word, the existing liquid sample processing methods increase operation steps, increase workload of inspectors, reduce automation degree of a full-automatic biochemical analyzer and reduce detection efficiency of the full-automatic biochemical analyzer.
Disclosure of Invention
The invention aims to overcome the defects of the prior art and provides a reaction disc of a full-automatic biochemical analyzer, wherein a reaction disc cover assembly is provided with n movable cup groove assemblies and m fixed cup groove assemblies. n is less than or equal to m, and n and m are even numbers. The reaction tray cover component is provided with N cup grooves which are divided into X cup grooves of the fixed bracket and Y cup grooves of the movable bracket. Each fixed cup slot component comprises a fixed bracket and X/m cup slots matched with the reaction cups. Each movable cup slot component comprises a movable bracket and Y/n cup slots matched with the reaction cups. The rotating shaft fixed at the edge of the inner side of the top of the movable bracket is inserted into a rotating shaft hole of a rotating shaft seat fixed on the reaction disc. When the reaction disc cover assembly rotates at a high speed, the movable bracket rotates along the rotating shaft under the action of centrifugal force, the bottom of the movable bracket turns over towards the outer upper part of the reaction disc, and sample liquid in the reaction cup in the cup groove of the movable bracket is centrifuged.
The invention also aims to provide a use method of the reaction disk of the full-automatic biochemical analyzer, which is characterized in that the sample liquid to be centrifuged is added into the reaction cups of the movable bracket cup grooves, the sample adding amount of each reaction cup is equal, so that Y movable bracket cup grooves are still in central symmetry with the center of the reaction disk, and the dynamic balance of the reaction disk cover assembly during rotation is ensured. The invention solves the problem of centrifugal treatment of liquid samples; the sample liquid is not required to be processed by other centrifugal instruments, the labor required by the test is reduced, the test efficiency is improved, and the real full-automatic biochemical analysis is realized.
The invention provides a full-automatic biochemical analyzer reaction disc which comprises a reaction disc cover assembly, a main shaft and a motor, wherein the motor is fixedly arranged on a biochemical analyzer chassis below the reaction disc cover assembly, and the main shaft is rotatably and vertically arranged on a biochemical analyzer rack. The output shaft of the motor is connected with the main shaft through the coupler, the center of the reaction disk cover component is arranged at the top of the main shaft, and the surface of the reaction disk cover is horizontal. The reaction disk cover component comprises a reaction disk cover, N square cup grooves which are uniformly distributed on the outer edge of the circumference of the reaction disk cover and have the same shape and size, and N same reaction cups matched with the cup grooves, wherein N is more than or equal to 16 and less than or equal to 512.
The reaction disk cover is provided with m fixed cup groove components, m is an even number, and m is more than or equal to 2 and less than or equal to 20. The reaction disk cover is provided with n movable cup groove components, n is an even number, and n is less than or equal to m. N cup grooves of the reaction tray cover assembly are divided into X cup grooves of the fixed cup groove assembly and Y cup grooves of the movable cup groove assembly, X is a multiple of m, Y is a multiple of N, and Y is smaller than or equal to X. The n movable cup groove components are separated by the m fixed cup groove components and are distributed in central symmetry with the center of the reaction disk cover.
The fixed cup slot components are fixed relative to the reaction disk cover, and each fixed cup slot component comprises a fixed bracket and X/m cup slots matched with the reaction cups. The fixed bracket is an arc bracket matched with the reaction disk cover, and the inner side of the top of the fixed bracket is fixed at the outer edge of the reaction disk cover. The reaction cup is inserted into the cup groove of the fixed bracket, and the central line of the reaction cup is vertical to the disk surface of the reaction disk cover.
The movable cup groove component comprises a movable bracket and Y/n cup grooves matched with the reaction cups. The activity bracket is with reaction dish lid matched with arc bracket, and 1 ~ 3 pivots are fixed to the inboard edge in activity bracket top.
The connecting line of the midpoint of the connecting line of the 1 rotating shaft or the plurality of rotating shafts on the same movable bracket and the center of the reaction disk cover is vertical to the rotating shaft or the plurality of rotating shafts; the mounting position of the rotating shaft is symmetrical with the central vertical plane of the inner side edge of the top of the movable bracket.
The edge of the reaction tray cover is fixed with a rotating shaft seat matched with the rotating shaft, the rotating shaft seat is provided with a rotating shaft hole, the rotating shaft is rotatably inserted in the rotating shaft hole of the rotating shaft seat, and the movable bracket is movably connected with the outer edge of the reaction tray cover through the rotating shaft and the rotating shaft seat. When the reaction tray cover component is static or rotates at low speed, the central line of the reaction cup inserted in the movable bracket cup groove is vertical to the surface of the reaction tray, and the reaction cup in the movable bracket cup groove and the reaction cup in the fixed bracket cup groove are at the same horizontal height. When the reaction tray cover assembly rotates at a high speed, the movable bracket rotating at a high speed along the rotating shaft is acted by centrifugal force, the bottom of the movable bracket is turned over to the outside and the upper part of the reaction tray cover, and the intersection angle of the central line of the cup groove and the reaction cup and the horizontal plane is 120-140 degrees.
The speed of the low-speed rotation of the reaction disk cover component is equal to or less than 20r/min, and the speed of the high-speed rotation of the reaction disk cover component is greater than or equal to 1200 r/min.
The edge of the reaction disc cover is provided with 1-3 grooves matched with the rotating shaft seat, and the rotating shaft seat is fixedly arranged in the grooves.
The motor is a servo motor.
The cup groove of the movable bracket is the same as that of the fixed bracket, the radial length of the cup groove is 1-2 mm larger than that of the reaction cup, a pre-tightening elastic sheet is arranged on the outer side wall of the cup groove, a fixing piece is arranged on the upper portion of the pre-tightening elastic sheet, and a forked elastic sheet is connected below the fixing piece. The elastic sheet is continuously bent twice, and takes the Jiong shape as a fixing piece when viewed from side, the fixing piece is clamped and hung on the top of the outer side wall of the cup groove, and the lower fork-shaped elastic sheet extends into the inner cavity of the cup groove. After the reaction cup is inserted into the cup groove, the forked elastic sheet at the lower part of the pre-tightening elastic sheet is pressed, and the reaction cup is pushed to the groove wall close to the inner side of the cup groove by the elastic force to be positioned.
The outer side top of the movable bracket and the outer side top of the fixed bracket are lower than the upper edge of the cup groove to form a step, the arc-shaped pressing block is fixed on the step, and presses the Jiong-shaped top of the pre-tightening elastic sheet fixing piece positioned on the wall top of the cup groove to prevent the pre-tightening elastic sheet from being taken out and falling off when the reaction cup is taken out.
The circular arc-shaped balancing weight is fixedly arranged on the inner side of the bottom of the movable bracket to adjust the gravity center of the movable cup groove component, so that the turning angle of the movable bracket when the reaction tray cover component rotates at high speed is adjusted.
The edge of the reaction disk cover is provided with a convex edge extending out of the movable bracket, the inner edge of the top of the movable bracket is provided with an upper brim protruding out of the reaction disk cover, when the reaction disk cover assembly is static or rotates at low speed, the upper brim of the movable bracket is lapped on the convex edge of the reaction disk cover, and the lower surface of the upper brim is attached to the upper surface of the convex edge, so that the reaction cup in the movable bracket and the reaction cup in the fixed bracket are at the same horizontal height when the reaction disk assembly is static or rotates at low speed, and the biochemical test is convenient.
The reaction disc of the full-automatic biochemical analyzer further comprises a heating ring assembly, the heating ring assembly comprises a heating cover, a heating ring and a temperature sensor, the heating cover covers the reaction disc cover assembly, the heating cover is a cylinder with the inner diameter larger than that of the reaction disc cover, the bottom of the cylinder is fixedly connected with a reaction disc cover bottom plate through a support column, and the heating cover is coaxial with the main shaft. The top of the cylinder is higher than the highest height of the movable reaction cup assembly after being turned up when the reaction disc cover assembly rotates at a high speed by 3-5 mm. The heating ring arranged on the inner wall of the cylinder is internally provided with uniformly distributed heating wires, the electromagnetic switch of the heating wires is connected with the control center of the biochemical analyzer to be controlled to be switched on or off, the temperature sensor is arranged in the heating cover, and the signal wire of the temperature sensor is connected with the input end of the biochemical analyzer. The biochemical analyzer control center controls the switch of the electric heating wire to be switched on or off according to the temperature signal of the temperature sensor, and controls the temperature inside the heating cover so that each reaction cup of the reaction plate cover assembly is at a proper temperature during testing.
The use method of the reaction disc of the full-automatic biochemical analyzer comprises the following main steps:
i, adding a liquid sample to be centrifuged
The biochemical analyzer control center controls the sample adding arm to add sample liquid to be centrifuged into reaction cups of Y cup grooves of the movable bracket, Y is not more than Y, Y is a multiple of n, and the sample adding amount of the Y reaction cups is equal, so that the Y cup grooves of the movable bracket are centrosymmetric by using the center of the reaction disk cover, and the dynamic balance of the reaction disk cover assembly during rotation is ensured.
II, centrifuging
The control center controls the motor to drive the main shaft to rotate so as to drive the reaction disk cover assembly to rotate at a high speed, the movable bracket is subjected to centrifugal force, the bottom of the movable bracket is turned up towards the outside and the upper part of the reaction disk cover, and the intersection angle of the inner center line of the movable bracket and the horizontal plane is 120-140 degrees.
The centrifugal force F is dependent on the radius of rotation r, the speed of rotation omega and the quantity m of the liquid sample
F＝mω2r
In order to achieve a good centrifugal effect, the rotating speed of the reaction disc cover component is 1200 r/min-1800 r/min, and the duration time is 3-8 minutes.
III, sample separation
After the motor is powered off, standing for 0.5-3 minutes, controlling the motor by a biochemical instrument control center to enable the reaction disc cover assembly to rotate at a low speed, and controlling the sample adding arm to distribute the centrifuged supernatant sample liquid in the y reaction cups to the reaction cups of the fixed cup-slot assemblies for preparing to carry out each test item according to the test requirement;
IV, testing
And the control center of the biochemical analyzer controls the reaction disc cover assembly and the sample adding arm according to the conventional method, sequentially adds reagents, stirs and reacts according to test requirements, and sequentially sends the reaction products to the detection hole sites for detection after the required reaction is completed.
Compared with the prior art, the reaction disc of the full-automatic biochemical analyzer and the using method thereof have the advantages that: 1. the movable bracket on the reaction tray cover assembly can be turned upwards when the motor rotates at a high speed, so that the reaction tray cover assembly and the centrifugal machine are combined into a whole, and the centrifugal treatment problem of the liquid sample is solved; 2. the sample liquid is not required to be processed by other centrifugal instruments, so that the labor required by the test is reduced, the test efficiency is improved, and the real full-automatic biochemical analysis is realized; 3. the spindle motor of the reaction disc directly drives the reaction disc to rotate at a high speed, the highest rotating speed can reach 6000r/min, the centrifugal force required by sample liquid is completely met, the structure is simple, and the installation is convenient; and the movement is stable without obvious noise.
Drawings
FIG. 1 is a front sectional view of the whole structure of the reaction disk of the full-automatic biochemical analyzer;
FIG. 2 is a front sectional view of the whole structure of the reaction disk of the full-automatic biochemical analyzer in which the spindle rotates at a high speed;
FIG. 3 is a schematic view of the reaction tray cover assembly of FIG. 1;
FIG. 4 is a schematic view of the movable cup and tank assembly of FIG. 1;
FIG. 5 is a top view of the reaction tray cover assembly of FIG. 1;
FIG. 6 is an enlarged view of portion A of FIG. 5;
FIG. 7 is a schematic diagram showing the relationship between the movable cup and cover of the reaction plate of the full-automatic biochemical analyzer;
FIG. 8 is an enlarged view of portion B of FIG. 7;
FIG. 9 is a schematic view of a pre-tightening spring of a reaction cup of a reaction plate of a full-automatic biochemical analyzer.
The reference numbers in the figures are:
1. reaction plate cover assembly, 11, reaction plate cover, 111, rotating shaft seat, 12, fixed cup groove assembly, 13, movable cup groove assembly, 131, movable bracket, 132, rotating shaft, 133, pressing block, 134, balancing weight, 2, main shaft, 3, heating ring assembly, 4, heating ring supporting column, 5, reaction plate bottom plate, 6, motor, 7, cup groove, 71, pre-tightening elastic sheet, 8, reaction cup
Detailed Description
To further illustrate the detailed concept and structure of the present invention, the following detailed description is made with reference to the accompanying drawings.
Embodiments of reaction plate of full-automatic biochemical analyzer
The whole structure of the reaction disk of the full-automatic biochemical analyzer is shown in fig. 1 and fig. 2, and comprises a reaction disk cover assembly 1, a spindle 2, a heating ring assembly 3 and a motor 6, wherein the motor 6 is a servo motor. The motor 6 is fixedly arranged on the chassis of the biochemical analyzer below the reaction disc cover component 1, and the main shaft 2 is vertically arranged on the frame of the biochemical analyzer in a rotatable way. The output shaft of the motor 6 is connected with the main shaft 2 through a coupler, the center of a reaction disk cover 11 of the reaction disk cover component 1 is arranged at the top of the main shaft 2, and the surface of the reaction disk cover 11 is horizontal. The reaction disk cover assembly 1 of this embodiment comprises a reaction disk cover 11, 120 square cup grooves 7 with the same shape and size and 120 same reaction cups 8 matched with the cup grooves, which are uniformly distributed on the circumference outer edge of the reaction disk cover 11.
As shown in fig. 3, the reaction disk cover 11 of this embodiment is provided with 4 fixed cup assemblies 12 which are fixed with respect to the reaction disk cover 11, and the reaction disk cover 11 of this embodiment is provided with 2 movable cup assemblies 13. The 2 movable cup groove components 13 are separated by the 2 fixed cup groove components 12, the distribution sequence on the circumference of the reaction disk cover 11 is 'movable cup groove components 13', 2 'fixed cup groove components 12', and each cup groove component is distributed in central symmetry with the center of the reaction disk cover 11.
Each fixed cup and groove assembly 112 comprises a fixed bracket and 20 cup grooves 7 matched with reaction cups 8. The fixed bracket is an arc bracket matched with the reaction disk cover 11, and the inner side of the top of the fixed bracket is fixed on the outer edge of the reaction disk cover 11 through a positioning pin. The reaction cup 8 is inserted into the cup groove of the fixed bracket, and the central line of the reaction cup 8 is vertical to the disk surface of the reaction disk cover 11.
Each movable cup slot assembly 13 comprises a movable bracket 131 and 20 cup slots 7 matched with reaction cups 8. As shown in fig. 4 to 6, the movable bracket 131 of each movable cup and groove assembly 13 is an arc bracket that is matched with the reaction plate cover 11, and 2 rotating shafts 132 are fixed on the inner side edge of the top of the movable bracket 131.
The connecting line of the middle point of the connecting line of the 2 rotating shafts on the same movable bracket 131 and the center of the reaction disk cover is vertical to the rotating shaft; the installation positions of the 2 rotating shafts 132 are symmetrical by taking the central vertical plane of the inner side edge of the top of the movable bracket 131 as a plane;
the edge of the reaction disk cover 11 of this embodiment is fixed with a rotating shaft seat 111 matching with a rotating shaft 132 on a movable bracket 131, the rotating shaft seat 111 is provided with a rotating shaft hole, the rotating shaft 132 is rotatably inserted in the rotating shaft hole of the rotating shaft seat 111, and the movable bracket 131 is movably connected with the outer edge of the reaction disk cover 11 through the rotating shaft 132 and the rotating shaft seat 111. When the reaction tray cover assembly 1 is static or rotates at a low speed, the cup groove of the movable bracket and the central line of the reaction cup in the cup groove are vertical to the tray surface of the reaction tray cover 11, and the reaction cup in the cup groove of the movable bracket 131 and the reaction cup in the cup groove of the fixed bracket are at the same horizontal height. When the reaction tray cover assembly 1 rotates at a high speed, the movable bracket 131 also rotates at a high speed, the movable bracket rotates upwards and outwards along the rotating shaft 132 under the action of centrifugal force, the bottom of the movable bracket 131 is turned upwards and outwards of the reaction tray cover 11, and the intersection angle between the inner cup groove and the center line of the reaction cup and the horizontal plane is 120-140 degrees.
The speed of the low-speed rotation of the reaction disk cover assembly 1 is equal to or less than 20r/min, and the speed of the high-speed rotation of the reaction disk cover assembly 1 is greater than or equal to 1200 r/min.
The reaction disk cover 11 of this embodiment has 2 grooves on its edge for engaging with the rotating shaft seat 111, and the rotating shaft seat 111 is fixedly mounted in the grooves.
In this embodiment, the cup grooves of the movable bracket and the fixed bracket are the same, as shown in fig. 9, the radial length of the cup groove 7 is 1-2 mm larger than that of the reaction cup 8, the outer side wall of the cup groove 7 is provided with a pre-tightening elastic sheet 71, the upper part of the pre-tightening elastic sheet 71 is provided with a fixing member 711, and a forked elastic sheet 712 is arranged below the fixing member 711. The elastic sheet is bent twice continuously, and is Jiong-shaped as a fixing member 711 in side view, the fixing member 711 is clamped and hung at the top of the outer side wall of the cup slot 7, and the lower fork-shaped elastic sheet 712 extends into the inner cavity of the cup slot 7. After the reaction cup 8 is inserted into the cup slot 7, the forked elastic sheet at the lower part of the pre-tightening elastic sheet 71 is pressed, and the reaction cup 8 is pushed to the inner side of the cup slot 7 by the elastic force to be positioned.
The outer side wall of the cup groove 7 is lower than the upper edge of the cup groove 71 to form a step, the arc-shaped pressing block 133 is fixed on the step, the pressing block 133 presses the top of the pre-tightening elastic sheet 71 fixing piece 711 Jiong on the wall top of the cup groove 7, and the pre-tightening elastic sheet 71 cannot be taken out and fall off when the reaction cup 8 is taken out.
The circular arc-shaped balancing weight 134 is fixedly arranged at the inner side of the bottom of the movable bracket 131 to adjust the gravity center of the movable cup slot assembly 13, so as to adjust the turning angle of the movable bracket 131 when the reaction tray cover assembly 1 rotates at a high speed.
The edge of the reaction disk cover 11 is provided with a convex edge extending out of the movable bracket 131, the inner edge of the top of the movable bracket 131 is provided with an upper brim protruding out of the reaction disk cover 11, when the reaction disk cover assembly 1 is static or rotates at a low speed, the upper brim of the movable bracket 131 is lapped on the convex edge of the reaction disk cover 1, the lower surface of the upper brim is attached to the upper surface of the convex edge, so that the reaction cup 8 in the movable bracket is at the same horizontal height as the reaction cup 8 in the fixed bracket when the reaction disk cover assembly 1 is static or rotates at a low speed, and biochemical tests are facilitated.
The heating ring component of the reaction disk of the full-automatic biochemical analyzer comprises a heating cover, a heating ring and a temperature sensor, wherein the heating cover covers the reaction disk cover component 1, the heating cover is a cylinder with the inner diameter larger than that of the reaction disk cover, the bottom of the cylinder is connected and fixed with a reaction disk cover bottom plate 5 through a heating ring support column 4, and the heating cover is coaxial with the main shaft 2. The top of the cylinder is higher than the highest height of the turnover movable cup groove component 13 after the reaction disc cover component 1 rotates at a high speed by 3-5 mm. The heating ring arranged on the inner wall of the heating cover cylinder is internally provided with uniformly distributed heating wires, an electromagnetic switch of each heating wire is connected with a control center of the biochemical analyzer to be controlled to be switched on or off, the temperature sensor is arranged in the heating cover, and a signal wire of the temperature sensor is connected with the input end of the biochemical analyzer. The biochemical analyzer control center controls the switch of the electric heating wire to be switched on or off according to the temperature signal of the temperature sensor, and controls the temperature inside the heating cover, so that each reaction cup 8 of the reaction disk cover assembly 1 is at a proper temperature during testing.
The embodiment of the using method of the reaction disk of the full-automatic biochemical analyzer adopts the embodiment 1 or 2 of the reaction disk of the full-automatic biochemical analyzer, and comprises the following main steps:
i, adding a liquid sample to be centrifuged
The biochemical analyzer control center controls the sample adding arm to add sample liquid to be centrifuged into the reaction cups 8 of the Y cup grooves of the movable bracket 131, Y is not more than Y, Y is a multiple of n, and the sample adding amount of the Y reaction cups 8 is equal, so that the movable bracket 131 is centrosymmetric with the center of the reaction disk cover 11, and the dynamic balance of the reaction disk cover assembly 1 during rotation is ensured.
II, centrifuging
The control center controls the motor 6 to drive the main shaft 2 to rotate, drives the reaction disk cover component 1 to rotate at high speed, the movable bracket 131 is subjected to centrifugal force, the bottom of the movable bracket is turned up towards the outside and the upper part of the reaction disk cover 11, and the intersection angle of the central line of the inner reaction cup 8 and the horizontal plane is 120-140 degrees.
The centrifugal force F is dependent on the radius of rotation r, the speed of rotation omega and the quantity m of the liquid sample
F＝mω2r
In order to achieve a good centrifugal effect, the rotating speed of the reaction disc cover component 1 is 1200 r/min-1800 r/min, and the duration time is 3-8 minutes.
III, sample separation
After the motor 6 is powered off, standing for 0.5-3 minutes, controlling the motor 6 by the biochemical instrument control center to enable the reaction disc cover assembly 1 to rotate at a low speed, and controlling the sample adding arm to distribute the centrifuged supernatant sample clear liquid in the y reaction cups 8 to the reaction cups 8 of the fixed cup slot assemblies 12 for carrying out each test item according to the test requirement;
IV, testing
And the control center of the biochemical analyzer controls the reaction disc cover component 1 and the sample adding arm according to the conventional method, sequentially adds reagents, stirs and reacts according to the test requirements, and sequentially sends the reaction products to the detection hole sites for detection after the required reaction is completed.
The above-described embodiments are only specific examples for further explaining the object, technical solution and advantageous effects of the present invention in detail, and the present invention is not limited thereto. Any modification, equivalent replacement, improvement and the like made within the scope of the disclosure of the present invention are included in the protection scope of the present invention.
Claims (9)
1. A reaction disc of a full-automatic biochemical analyzer comprises a reaction disc cover assembly (1), a main shaft (2) and a motor (6), wherein the motor (6) is fixedly arranged on a biochemical analyzer chassis below the reaction disc cover assembly (1), and the main shaft (2) is rotatably and vertically arranged on a biochemical analyzer rack; an output shaft of the motor (6) is connected with the main shaft (2) through a coupler, the center of a reaction disk cover (11) of the reaction disk cover component (1) is arranged at the top of the main shaft (2), and the surface of the reaction disk cover (11) is horizontal; the reaction disc cover assembly (1) comprises a reaction disc cover (11), N square cup grooves (7) which are uniformly distributed on the outer edge of the circumference of the reaction disc cover (11) and have the same shape and size, and N same reaction cups (8) which are matched with the cup grooves (7), wherein N is more than or equal to 16 and less than or equal to 512; the method is characterized in that:
the reaction disk cover (11) is provided with m fixed cup groove components (12), m is an even number, and m is more than or equal to 2 and less than or equal to 20; the reaction disk cover (11) is provided with n movable cup groove components (13), n is an even number and is less than or equal to m; the N cup grooves (7) of the reaction tray cover assembly (1) are divided into X cup grooves of the fixed cup groove assembly (12) and Y cup grooves of the movable cup groove assembly (13), wherein X is a multiple of m, Y is a multiple of N, and Y is smaller than or equal to X; the n movable cup groove components (13) are separated by the m fixed cup groove components (12) and are distributed in a centrosymmetric way by taking the center of the reaction disk cover (11);
the fixed cup slot assemblies (12) are fixed relative to the reaction disk cover (11), and each fixed cup slot assembly (12) comprises a fixed bracket and X/m cup slots (7) which are matched with the reaction cups (8) on the fixed bracket; the fixed bracket is an arc bracket matched with the reaction disk cover (11), and the inner side of the top of the fixed bracket is fixed at the outer edge of the reaction disk cover (11); the reaction cup (8) is inserted into the cup groove of the fixed bracket, and the central line of the reaction cup (8) is vertical to the disc surface of the reaction disc cover (11);
the movable cup slot component (13) comprises a movable bracket (131) and Y/n cup slots (7) which are arranged on the movable bracket and matched with the reaction cups (8);
the movable bracket (131) of each movable cup groove component (13) is an arc bracket matched with the reaction disc cover (11), 1-3 rotating shafts (132) are fixed at the edge of the inner side of the top of the movable bracket (131), the center lines of the rotating shafts (132) are positioned on the same straight line parallel to the surface of the reaction disc cover (11), a rotating shaft seat (111) matched with the rotating shafts (132) is fixed at the edge of the reaction disc cover (11), a rotating shaft hole is formed in the rotating shaft seat (111), the rotating shafts (132) are rotatably inserted into the rotating shaft hole of the rotating shaft seat (111), and the movable bracket (131) is movably connected with the outer edge of the reaction disc cover (11) through the rotating shafts (132) and the rotating shaft seat (111); when the reaction disk cover component (1) is static or rotates at low speed, the central line of the reaction cup (8) inserted in the cup groove (7) of the movable bracket (131) is vertical to the disk surface of the reaction disk cover (11), and the reaction cup (8) in the cup groove (7) of the movable bracket (131) and the reaction cup (8) in the cup groove of the fixed bracket are at the same horizontal height; when the reaction tray cover assembly (1) rotates at a high speed, the movable bracket (131) rotates along the rotating shaft (132) under the action of centrifugal force, the bottom of the movable bracket (131) is turned up to the outside and the upper part of the reaction tray cover (11), and the intersection angle of the central line of the reaction cup (8) in the movable bracket and the horizontal plane is 120-140 degrees;
the edge of the reaction disc cover (11) is provided with 1-3 grooves matched with the rotating shaft seat (111), and the rotating shaft seat (111) is fixedly arranged in the grooves;
the speed of the low-speed rotation of the reaction disk cover assembly (1) is equal to or less than 20r/min, and the speed of the high-speed rotation of the reaction disk cover assembly (1) is greater than or equal to 1200 r/min.
2. The reaction disk of the full-automatic biochemical analyzer according to claim 1, wherein:
the connecting line of the midpoint of the connecting lines of the 1 rotating shaft (132) or the plurality of rotating shafts (132) on the same movable bracket (131) and the center of the reaction disk cover (11) is vertical to the rotating shaft (132) or the plurality of rotating shafts (132); the installation position of the rotating shaft (132) is symmetrical to the central vertical plane of the inner side edge of the top of the movable bracket (131).
3. The reaction disk of the full-automatic biochemical analyzer according to claim 1, wherein:
the motor (6) is a servo motor.
4. The full-automatic biochemical analyzer reaction plate according to any one of claims 1 to 3, wherein:
the radial length of the cup groove (7) of the movable bracket (131) and the radial length of the cup groove (7) of the fixed bracket are 1-2 mm longer than that of the reaction cup (8), a pre-tightening elastic sheet (71) is installed on the outer side wall of the cup groove (7), a fixing piece is arranged at the upper part of the pre-tightening elastic sheet (71), and a fork-shaped elastic sheet is connected below the fixing piece; the elastic sheet is continuously bent twice and takes the Jiong shape as a fixing piece in side view, the fixing piece is clamped and hung on the top of the outer side wall of the cup groove (7), and the lower fork-shaped elastic sheet extends into the inner cavity of the cup groove (7); after the reaction cup (8) is inserted into the cup groove (7), the forked elastic sheet at the lower part of the pre-tightening elastic sheet (71) is pressed, and the reaction cup (8) is pushed to the inner side of the cup groove (7) by the elastic force to be positioned.
5. The reaction disk of the full-automatic biochemical analyzer according to claim 4, wherein:
the outer side tops of the movable bracket (131) and the fixed bracket, namely the outer side wall of the cup groove (7) is lower than the upper edge of the cup groove (7) to form a step, the arc-shaped pressing block (133) is fixed on the step, and the pressing block (133) presses the top of the fixing piece Jiong of the pre-tightening elastic sheet (71) positioned on the wall top of the cup groove (7).
6. The full-automatic biochemical analyzer reaction plate according to any one of claims 1 to 3, wherein:
the circular arc-shaped balancing weight (134) is fixedly arranged on the inner side of the bottom of the movable bracket (131).
7. The full-automatic biochemical analyzer reaction plate according to any one of claims 1 to 3, wherein:
the edge of the reaction disc cover (11) is provided with a convex edge extending out of the movable bracket (131), the inner edge of the top of the movable bracket (131) is provided with an upper brim protruding out of the reaction disc cover (11), when the reaction disc cover (11) is static or rotates at a low speed, the upper brim of the movable bracket (131) is lapped on the convex edge of the reaction disc cover (11), the lower surface of the upper brim is attached to the upper surface of the convex edge, so that the reaction cup (8) in the movable bracket (131) is at the same horizontal height as the reaction cup (8) in the fixed bracket when the reaction disc assembly (1) is static or rotates at a low speed.
8. The full-automatic biochemical analyzer reaction plate according to any one of claims 1 to 3, wherein:
the reaction disc of the full-automatic biochemical analyzer further comprises a heating ring assembly (3), the heating ring assembly (3) comprises a heating cover, a heating ring and a temperature sensor, the heating cover covers the reaction disc assembly (1), the heating cover is a cylinder with the inner diameter larger than that of the reaction disc cover (11), the bottom of the cylinder is fixedly connected with a bottom plate of the reaction disc cover (11) through a heating ring support column (4), and the heating cover is coaxial with the main shaft (2); the top of the cylinder is higher than the highest height of the reaction cup (8) of the movable bracket (131) after being turned up when the reaction disc cover (11) rotates at a high speed by 3-5 mm; the heating ring arranged on the inner wall of the cylinder is internally provided with uniformly distributed heating wires, the electromagnetic switch of the heating wires is connected with the control center of the biochemical analyzer to be controlled to be switched on or off, the temperature sensor is arranged in the heating cover, and the signal wire of the temperature sensor is connected with the input end of the biochemical analyzer.
9. The method for using the reaction disk of the full-automatic biochemical analyzer according to any one of claims 1 to 3, characterized by comprising the following main steps:
i, adding a liquid sample to be centrifuged
The biochemical analyzer control center controls the sample adding arm to add sample liquid to be centrifuged into reaction cups (8) of Y cup grooves of the movable bracket (131), Y is less than or equal to Y, Y is a multiple of n, and the sample adding amount of the Y reaction cups (8) is equal, so that the Y cup grooves of the movable bracket (131) are in central symmetry with the center of the reaction disk cover (11), and the dynamic balance of the reaction disk cover assembly (1) during rotation is ensured;
II, centrifuging
The control center controls a motor (6) to drive a main shaft (2) to rotate so as to drive a reaction disk cover assembly (1) to rotate at a high speed, a movable bracket (131) is subjected to centrifugal force, the bottom of the movable bracket is turned up towards the outer upper part of the reaction disk cover, and the intersection angle of the central line of an inner reaction cup (8) and the horizontal plane is 120-140 degrees;
the rotating speed of the reaction disc cover (11) is 1200 r/min-1800 r/min, and the duration time is 3-8 minutes;
III, sample separation
After the motor (6) is powered off, standing for 0.5-3 minutes, controlling the motor (6) by a biochemical analyzer control center to enable the reaction disc cover (11) to rotate at a low speed, and controlling the sample adding arm to distribute the centrifuged supernatant sample clear liquid in the y reaction cups (8) to the reaction cups (8) of the fixed cup and groove assemblies (13) for carrying out each test item according to the test requirement;
IV, testing
And the control center of the biochemical analyzer controls the reaction disc cover component (1) and the sample adding arm according to the conventional method, sequentially adds reagents, stirs and reacts according to the test requirements, and sequentially sends the reaction products to the detection hole sites for detection after the required reaction is completed.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811035109.3A CN109188007B (en) | 2018-09-06 | 2018-09-06 | Reaction disc of full-automatic biochemical analyzer and use method thereof |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201811035109.3A CN109188007B (en) | 2018-09-06 | 2018-09-06 | Reaction disc of full-automatic biochemical analyzer and use method thereof |
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| CN109188007A CN109188007A (en) | 2019-01-11 |
| CN109188007B true CN109188007B (en) | 2022-04-12 |
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| US3605829A (en) * | 1970-04-29 | 1971-09-20 | Becton Dickinson Co | Blood handling machine |
| FR2858251B1 (en) * | 2003-07-29 | 2005-10-28 | Diagyr | METHOD FOR BALANCING A ROTARY TRAY OF A CENTRIFUGE AND CENTRIFUGE EMPLOYING THE PROCESS |
| WO2005057224A1 (en) * | 2003-12-08 | 2005-06-23 | Wako Pure Chemical Industries,Ltd. | Automatic analyzer-use reaction disc and separating cell |
| CN107621444B (en) * | 2017-08-30 | 2020-04-14 | 桂林优利特医疗电子有限公司 | Independent reaction cup fixing structure of biochemical analyzer |
| CN108061809A (en) * | 2017-11-27 | 2018-05-22 | 桂林优利特医疗电子有限公司 | Biochemical instruments react cup holding rack and reaction tray movement accuracy-control system |
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