CN109187517A - The prediction technique of chloroamides class degradation of pesticide rate in a kind of biogas residue returning to the field soil - Google Patents
The prediction technique of chloroamides class degradation of pesticide rate in a kind of biogas residue returning to the field soil Download PDFInfo
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- CN109187517A CN109187517A CN201810838473.7A CN201810838473A CN109187517A CN 109187517 A CN109187517 A CN 109187517A CN 201810838473 A CN201810838473 A CN 201810838473A CN 109187517 A CN109187517 A CN 109187517A
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
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Abstract
The invention belongs to Pesticide environment assessment technique field, a kind of prediction technique of chloroamides class degradation of pesticide rate in biogas residue returning to the field soil is provided.The present invention is aiming at the problem that biogas residue returning to the field influences chloroamides class degradation of pesticide rate, to the free solubilised state of Pesticide Residue in Soil after consideration biogas residue returning to the field, desaturase activity in soil, the influence of degradation time etc., carry out the absorption degradation experiment of chloroamides class pesticide under different biogas residue usage ratios, using the method for regression analysis, construct the prediction model that biogas residue returning to the field acts on chloroamides class degradation of pesticide rate in lower soil, and model is verified, confirm that model has preferable predictive ability, there is certain directive significance to the prediction of biogas residue returning to the field Pesticide Residue in Soil degradation rate.
Description
Technical field
The invention belongs to Pesticide environment assessment technique fields, and in particular to a kind of prediction chloroamides class pesticide biogas residue also
The model and method of field degraded in soil rate.
Background technique
Biogas residue is a kind of solid residue that the agriculture and forestry organic waste materials such as human and animal excreta, stalk generate after anaerobic fermentation, is contained
Nutrient needed for having a large amount of plant growth, wherein containing organic matter 36.0%~50.0%, full nitrogen 0.78%~1.61%, full phosphorus
0.4%~0.6%, full potassium 0.61%~1.30% and other microelements etc. can be used as a kind of good base manure, improve soil
The content of organic matter improves soil microbial activities, while discharging the micronutrient element of multiple beneficial.Patent CN 107673873
A discloses a kind of method that ecological biogas residue fertilizer is made in biogas residue, illustrates that natural pond fertilizer has the function of quick-acting and slow two kinds of effect, can be used as base
Fertilizer and top dressing application；107954781 A of patent CN discloses a kind of a kind of method that biogas residue prepares slow-release compound fertilizer, utilizes natural pond
Slag and inorganic fertilizer compounding are prepared for the citrus compound fertilizer with slow-release function, and the utilization rate of fertilizer can be improved.Illustrate that biogas residue can
To be promoted the use of a large area in fertilizer field as a kind of good manure resources.
It can be the important leverage for maintaining grain yield to increase income, acyl along with the application of pesticide, pesticide during biogas residue returning to the field
Amine herbicide is one of indispensable pesticide of current increasing crop yield, is widely used in world's agricultural production at present, this
A little herbicides can be degraded by microorganisms in the soil, while also absorption-analytic function, shadow can occur with the organic matter in soil
The biological effectiveness process of pesticide in the soil is rung, and then influences the biological degradation rate of pesticide.Biogas residue is provided as a kind of biomass
Source will affect the absorption mass transfer and biodegradation process of Pesticide Residue in Soil containing loose porous organic matter after returning to the field.There is research
Show the pesticide after biogas residue returning to the field in adsorbable soil, influences its mass transfer and fractions distribution in the soil；And biogas residue returning to the field
After can also improve soil microenvironment, improve soil microbial activities, strengthen the biodegrade of pesticide.Biogas residue returning to the field at present
Relevant prediction model is lacked to the biodegrade of Pesticide Residue in Soil, the present invention constructs biogas residue returning to the field soil according to experimental data
The prediction model of middle chloroamides class degradation of pesticide rate, instructs the use of biogas residue returning to the field and pesticide.
Summary of the invention
The present invention is directed to influence of the biogas residue returning to the field to chloroamides class degradation of pesticide rate, constructs a kind of prediction chloroamides
The model and method of class degradation of pesticide rate.This method has comprehensively considered the free solubilised state of pesticide, desaturase activity in soil, pesticide drop
How each factors such as time are solved, and prediction model is verified.Wherein the free solubilised state of pesticide has been reacted pesticide available state and has been contained
Amount, this has certain guidance meaning to assessment pesticide biological effectiveness.
Technical solution of the present invention:
The prediction technique of chloroamides class degradation of pesticide rate in a kind of biogas residue returning to the field soil, steps are as follows:
(1) biogas residue returning to the field acts on the measurement of dehydrogenase activity in lower soil
Dehydrogenase activity uses first a ceremonial jade-ladle, used in libation colorimetric method for determining in soil；
(2) in biogas residue returning to the field soil chloroamides class degradation of pesticide rate and the free solubilised state of pesticide measurement
By the method for constant indoor temperature culture, biogas residue and soil mixing, biogas residue dosage account for the 0%~5% of soil quality, examine
Influence of the biogas residue usage ratio to degradation of pesticide rate is considered.Soil moisture content maintains the 60% of soil maximum moisture content；Pre- training
It is separately added into chloroamides class pesticide in soil after supporting, concentration is respectively 5mg/kg, dispenses after mixing, is put into artificial climate
It is cultivated in case, the temperature of culture maintains 25 DEG C, humidity 60%, no light；The chloroamides class pesticide be alachlor,
Acetochlor, isopropyl methoxalamine, butachlor and metalaxyl；The degradation rate of sampling analysis on time, pesticide is acquired by formula (1):
CDegradation rate=(CInitial concentration–CMeasured concentration)/CInitial concentration (1)
Soil uses balanced oscillations method to the absorption of chloroamides class pesticide；Set the initial concentration of chloroamides class pesticide
It is as follows: alachlor 20mg/L, Acetochlor 20mg/L, isopropyl methoxalamine 20mg/L, butachlor 10mg/L, metalaxyl 10mg/L；It takes
Soil in glass centrifuge tube, according to soil and water quality than mix: alachlor, Acetochlor and isopropyl methoxalamine be 1:5, butachlor and
Metalaxyl is 1:25, and chloroamides class pesticide includes the CaCl of 10mmol/L2Solution；It is vibrated under the conditions of 25 DEG C, 180r/min
For 24 hours, it after standing 2h, pipettes supernatant liquor and is filtered with 0.45 μm of water system film, measure chloroamides class pesticide with high performance liquid chromatography
Residual quantity in filtrate；Adsorption isotherm Freundlich equation is established according to adsorpting data, shown in the equation such as formula (2):
In formula, Q is the adsorbance mgkg of pesticide in the soil-1；CeWhen for adsorption equilibrium in water phase pesticide concentration
mg·L-1；KfIt is constant related with temperature with 1/n；
According to the experiment of chloroamides class degradation of pesticide and adsorption experiment, the free solubilised state of chloroamides class pesticide is by formula (3)
It acquires:
fFree solubilised state=(CeV/Ctm) (3)
In formula, CtFor Pesticide Residue in Soil instantaneous concentration；CeFor pesticide in the soil adsorption equilibrium when liquid phase concentration, V soil
Middle moisture content, m are the dry weight of soil, CeBy formula Ct=kfCe 1/n+V Ce/ m is acquired, kfIt is pesticide adsorption isotherm mould with 1/n
Constant related with temperature in type Freundlich equation；
(3) building of chloroamides class degradation of pesticide rate prediction model
Using chloroamides class degradation of pesticide rate as dependent variable, with the free solubilised state of pesticide, desaturase activity in soil, pesticide drop
The solution time is that independent variable progress regression analysis obtains optimal models, and the degradation rate of pesticide is calculated according to Regression Analysis Result, and
Analyze the linear fit result of measured value and predicted value；
Degradation prediction model:
logCDegradation rate=0.010logfFree solubilised state+0.158logADehydrogenase activity+0.436logtTime-0.489(R2=0.830)
Wherein, CDegradation rateFor the degradation rate of pesticide；fFree solubilised stateFor the free solubilised state of pesticide；ADehydrogenase activityIt is living for soil deterioration index
Property；tTimeFor the time of degradation of pesticide.
Beneficial effects of the present invention: the present invention is considering the free solubilised state of pesticide, desaturase activity in soil and degradation of pesticide
Under the conditions such as time, biogas residue usage ratio, using SPSS software regression analysis, chloroamides class under biogas residue returning to the field is constructed
The prediction model that degradation of pesticide rate changes over time, the model have the characteristics that predictive good, practical.
Detailed description of the invention
Fig. 1 is the experiment value and predicted value comparison diagram of model.
Specific embodiment
Below in conjunction with attached drawing and technical solution, a specific embodiment of the invention is further illustrated.
1 biogas residue of embodiment acts on the building of lower Acetochlor degradation rate prediction model
Using the free solubilised state of pesticide, desaturase activity in soil, degradation time as independent variable, degradation of pesticide rate is because becoming
Amount does not take log value respectively to each factor and uses input method to carry out regression model building after taking log value, and carries out model verifying.
Input method:
Take log value
logCDegradation rate=-0.068logfFree solubilised state+0.244logADehydrogenase activity+0.490logtTime-0.468(R2=0.852)
Log value is not taken
CDegradation rate=-2.854fFree solubilised state-0.673ADehydrogenase activity+0.017tTime+0.882(R2=0.776) finally selection takes log
The Acetochlor degradation prediction model of value building:
logCDegradation rate=-0.068logfFree solubilised state+0.244logADehydrogenase activity+0.490logtTime-0.468(R2=0.852) real
Apply the building that 2 biogas residue of example acts on lower isopropyl methoxalamine degradation rate prediction model
Using the free solubilised state of pesticide, desaturase activity in soil, degradation time as independent variable, degradation of pesticide rate is because becoming
Amount does not take log value respectively to each factor and uses input method to carry out regression model building after taking log value, and carries out model verifying.
Input method:
Take log value
logCDegradation rate=-0.090logfFree solubilised state+0.041logADehydrogenase activity+0.429logtTime-0.801(R2=0.965)
Log value is not taken
CDegradation rate=-3.369fFreely dissolve effect state-0.644ADehydrogenase activity+0.012tTime+0.707(R2=0.890)
Finally selection takes the isopropyl methoxalamine degradation rate prediction model of log value building:
logCDegradation rate=-0.090logfFree solubilised state+0.041logADehydrogenase activity+0.429logtTime-0.801(R2=0.965)
3 biogas residue of embodiment acts on the building of lower Butachlor degradation rate prediction model
Using the free solubilised state of pesticide, desaturase activity in soil, degradation time as independent variable, degradation of pesticide rate is because becoming
Amount does not take log value respectively to each factor and uses input method to carry out regression model building after taking log value, and carries out model verifying.
Input method:
Take log value
logCDegradation rate=0.016logfFree solubilised state+0.132logADehydrogenase activity+0.445logtTime-0.458(R2=0.943)
Log value is not taken
CDegradation rate=-1.379fFree solubilised state-0.125ADehydrogenase activity+0.025tTime+0.484(R2=0.890)
Finally selection takes the Butachlor degradation rate prediction model of log value building:
logCDegradation rate=0.016logfFree solubilised state+0.132logADehydrogenase activity+0.445logtTime-0.458(R2=0.965).
Claims (1)
1. the prediction technique of chloroamides class degradation of pesticide rate in a kind of biogas residue returning to the field soil, which is characterized in that steps are as follows:
(1) biogas residue returning to the field acts on the measurement of dehydrogenase activity in lower soil
Dehydrogenase activity uses first a ceremonial jade-ladle, used in libation colorimetric method for determining in soil；
(2) in biogas residue returning to the field soil chloroamides class degradation of pesticide rate and the free solubilised state of pesticide measurement
By the method for constant indoor temperature culture, biogas residue and soil mixing, biogas residue account for the 0%~5% of soil quality, soil moisture content
Maintain the 60% of soil maximum moisture content；Amides pesticide is separately added into soil after preculture, each concentration is 5mg/kg,
It dispenses after mixing, is put into growth cabinet and cultivates, the temperature of culture maintains 25 DEG C, humidity 60%, no light；Institute
The chloroamides class pesticide stated is alachlor, Acetochlor, isopropyl methoxalamine, butachlor and metalaxyl；Sampling analysis on time, pesticide
Degradation rate acquired by formula (1):
CDegradation rate=(CInitial concentration–CMeasured concentration)/CInitial concentration (1)
Soil uses balanced oscillations method to the absorption of chloroamides class pesticide；The initial concentration for setting pesticide is as follows: alachlor
20mg/L, Acetochlor 20mg/L, isopropyl methoxalamine 20mg/L, butachlor 10mg/L, metalaxyl 10mg/L；Take soil in glass from
In heart pipe, according to soil and water quality than mixing: alachlor, Acetochlor and isopropyl methoxalamine are 1:5, butachlor and metalaxyl are 1:
25, chloroamides class pesticide includes the CaCl of 10mmol/L2Solution；It is vibrated under the conditions of 25 DEG C, 180r/min for 24 hours, stands 2h
Afterwards, it pipettes supernatant liquor to be filtered with 0.45 μm of water system film, with residual quantity of the high performance liquid chromatography measurement pesticide in filtrate；According to
Adsorpting data establishes adsorption isotherm Freundlich equation, shown in the equation such as formula (2):
In formula, Q is the adsorbance mgkg of pesticide in the soil-1；CeWhen for adsorption equilibrium in water phase pesticide concentration mgL-1；
KfIt is constant related with temperature with 1/n；
It is acquired according to the experiment of chloroamides class degradation of pesticide and adsorption experiment, the free solubilised state of chloroamides class pesticide by formula (3):
fFree solubilised state=(CeV/Ct m) (3)
In formula, CtFor Pesticide Residue in Soil instantaneous concentration；CeFor pesticide in the soil adsorption equilibrium when liquid phase concentration, V soil water-containing
Rate, m are the dry weight of soil, CeBy formula Ct=kf Ce 1/n+V Ce/ m is acquired, kfIt is pesticide adsorption isotherm line model with 1/n
Constant related with temperature in Freundlich equation；
(3) building of amides pesticide degradation rate prediction model
It is from change with the free solubilised state of pesticide, desaturase activity in soil, degradation time using amides pesticide degradation rate as dependent variable
Amount carries out regression analysis and obtains optimal models, calculates the degradation rate of pesticide according to Regression Analysis Result, and analyze measured value with
The linear fit result of predicted value；
Degradation prediction model:
logCDegradation rate=0.010logfFree solubilised state+0.158logADehydrogenase activity+0.436logtTime-0.489 R2=0.830
Wherein, CDegradation rateFor the degradation rate of pesticide；fFree solubilised stateFor the free solubilised state of pesticide；ADehydrogenase activityFor desaturase activity in soil；tTime
For the time of degradation of pesticide.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112037867A (en) * | 2020-09-24 | 2020-12-04 | 大连理工大学 | Prediction method for pesticide leaching index in corn straw returning soil |
| CN113393907A (en) * | 2021-07-20 | 2021-09-14 | 西安交通大学 | Construction method and device of PPCPs organic pollutant degradation rate prediction model |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103373872A (en) * | 2012-04-16 | 2013-10-30 | 李晓莉 | Organic nutrient soil prepared from straw biogas residue and preparation method thereof |
| WO2015027209A2 (en) * | 2013-08-22 | 2015-02-26 | Kiverdi, Inc. | Microorganisms for biosynthesis of limonene on gaseous substrates |
| CN106416495A (en) * | 2016-09-04 | 2017-02-22 | 云南省能源研究院有限公司 | Method for effectively removing soil pesticide residues in microenvironment under plateau photovoltaic panel |
| CN106583443A (en) * | 2016-12-28 | 2017-04-26 | 青岛理工大学 | Method capable of synchronously treating biogas residues and pesticide polluted soil |
-
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103373872A (en) * | 2012-04-16 | 2013-10-30 | 李晓莉 | Organic nutrient soil prepared from straw biogas residue and preparation method thereof |
| WO2015027209A2 (en) * | 2013-08-22 | 2015-02-26 | Kiverdi, Inc. | Microorganisms for biosynthesis of limonene on gaseous substrates |
| CN106416495A (en) * | 2016-09-04 | 2017-02-22 | 云南省能源研究院有限公司 | Method for effectively removing soil pesticide residues in microenvironment under plateau photovoltaic panel |
| CN106583443A (en) * | 2016-12-28 | 2017-04-26 | 青岛理工大学 | Method capable of synchronously treating biogas residues and pesticide polluted soil |
Non-Patent Citations (1)
| Title |
|---|
| 刘萍等: "酰胺类农药在白洋淀典型农田土壤上的吸附行为", 《湖南科技大学学报（自然科学版）》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112037867A (en) * | 2020-09-24 | 2020-12-04 | 大连理工大学 | Prediction method for pesticide leaching index in corn straw returning soil |
| CN113393907A (en) * | 2021-07-20 | 2021-09-14 | 西安交通大学 | Construction method and device of PPCPs organic pollutant degradation rate prediction model |
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