CN109187367A - A kind of polarization optofluidic piece ballast water activity of microalgae detection device and method - Google Patents
A kind of polarization optofluidic piece ballast water activity of microalgae detection device and method Download PDFInfo
- Publication number
- CN109187367A CN109187367A CN201811333637.7A CN201811333637A CN109187367A CN 109187367 A CN109187367 A CN 109187367A CN 201811333637 A CN201811333637 A CN 201811333637A CN 109187367 A CN109187367 A CN 109187367A
- Authority
- CN
- China
- Prior art keywords
- module
- image
- microalgae
- micro
- analyzer
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/21—Polarisation-affecting properties
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/21—Polarisation-affecting properties
- G01N2021/216—Polarisation-affecting properties using circular polarised light
Landscapes
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating Or Analysing Materials By Optical Means (AREA)
Abstract
The invention discloses a kind of polarization optofluidic piece ballast water activity of microalgae detection device and method, device includes: light source module, detection module, image-forming module, image processing module, database module and the control module for the modules movement that is connected respectively with the light source module, image-forming module, the detection module of image, image processing module and database module, controls.The monochromatic source that the light source module provides is radiated on the camera of image processing module according to predetermined optical path by detection module and image-forming module, the relative rotary motion that control module passes through the polarizer and analyzer in control detection module simultaneously, so that camera is captured the microalgae cell image under different polarization states, and is analyzed and processed to obtain microalgae cell Activity Results for showing and storing by described image processing module.Technical solution of the present invention solves the problems, such as the problem of microalgae cell Activity determination is difficult, apparatus structure is complicated and inconvenient for use in the prior art.
Description
Technical field
The present invention relates to ballast water for ship activity of microalgae detection technique fields, specifically, more particularly to a kind of polarised light
Flow control piece ballast water activity of microalgae detection device and method.
Background technique
Ballast water for ship is the water and suspended material being attached on ship, effect be the heel for controlling ship, trim, drinking water,
Stability and stress.Ballast water can be seawater and be also possible to fresh water, and source can be harbour, river or open sea, and one
As delivered by the dedicated ballast tank either cargo hold unstowed.Ship must all fill ballast water in zero load, do so be for
Guarantee the stabilization that propeller and rudder are immersed in water the balance for guaranteeing the validity of operation, controlling ship, improve ship
Property, though be loaded with cargo or it is fully loaded when similarly need equipped with ballast water.When ship rests in harbour loading or unloading, all
It can the corresponding discharge and loading for carrying out ballast water.Ballast water for ship is one of the main path of external marine biological invasion, outside
Carry out the propagation of harmful marine biotic intrusion, serious influence is all caused on ecology, industry, agricultural and human health etc..In " ship
Ship ballast water and sediment monitoring and management international convention " in the mark that should meet of ballast water performance when defining ship discharge
Standard, wherein one of re-detection be different size viable biologicals in ballast water concentration.And planktonic organism value volume and range of product
(most generally existing a kind of biology in ballast water for ship), and the main indicator of ballast for cruising water process and detection at present.Greatly
Quantity research confirms that ballast water for ship is the main path and carrier of invasive plants, and the whole world exchanges ballast water about 120 every year
Hundred million tons, and survive in unprocessed ballast water biology up to 7000 kinds, ICES's statistics indicate that 94% potentially harmful sea
Foreign biology is propagated by ballast water for ship.
For the invasion for preventing adventive in ballast water, International Maritime Organization (IMO) formulated " international shipping in 2004
Ballast water and its sediment monitoring and management contract ", which will be formally effective on September 8th, 2017.As the pact will
It comes into force, China is as maximum ballast water exporting country and importer, it is necessary to carry out correlative study work.Contain in ballast water for ship
Bacterium, virus and a large amount of planktonic organism, wherein planktonic organism is based on microalgae, and these microalgaes are to lead to the main original of red tide
Cause.Microalgae is the small algal populations of a kind of form, widely distributed, is common in rivers,lakes and seas and pond irrigation canals and ditches, size is usually 2
Between μm -20 μm.The microalgae sea area new as ballast water for ship is discharged into, it is indomitable due to lacking natural enemy and vitality itself,
These adventives can bringing on a disaster property of the ecosystem to new sea area destroy, even result in the extinction of local endemic species, sternly
The safety of marine ecosystems is threatened again.It is external in ballast water for ship for analyzing that there is no a kind of preferable schemes and device at present
The activity of biology, therefore, the Activity determination problems demand of microalgae cell solves in ballast water for ship.
Summary of the invention
According to the technical problem of microalgae cell Activity determination difficulty in ballast water set forth above, and provide a kind of polarised light
Flow control piece ballast water activity of microalgae detection device.The present invention is mainly examined using activity of the polarization characteristic of light to microalgae cell
It surveys, to play the purpose quickly detected.
The technological means that the present invention uses is as follows:
A kind of polarization optofluidic piece ballast water activity of microalgae detection device characterized by comprising
The light source module of monochromatic light source is provided；
By the polarizer and analyzer relative rotation to change the microalgae sample that irradiation is to flow on micro-fluidic chip
On light direction of vibration, to obtain the detection module of image of the microalgae cell under different polarization states；
After being set to the detection module, the imaging lens group being made of two plano-convex lens is utilized to realize microalgae cell
The image-forming module of image amplification；
It is obtained using camera and is handled through image-forming module amplification, the microalgae cell image under different polarization states
It is acquired with polarization information, and the image processing module that all testing results are shown in display end；
It will test result and stored database module to be retrieved and to be analyzed to data at any time；
And respectively with the light source module, image-forming module, detection module, image processing module and database module phase
Even, the control module of control modules movement；
The monochromatic source that the light source module provides is radiated at figure by detection module and image-forming module according to predetermined optical path
As processing module camera on, while control module is dynamic by the relative rotation of the polarizer and analyzer in control detection module
Make, so that camera is captured the microalgae cell image under different polarization states, and carry out at analysis by described image processing module
Reason obtains microalgae cell Activity Results for showing and storing.
Further, the detection module includes the polarizer, analyzer and micro-fluidic chip；
The micro-fluidic chip includes setting gradually micro-fluidic chip body and glass slide, the micro-fluidic core from top to bottom
Piece main body fovea superior is carved with the sample channel that sample cell and waste liquid tank is respectively set in a head and the tail, and the micro-fluidic chip can control
The flow velocity of microalgae cell sample, consequently facilitating being shot to microalgae cell image；
The polarizer is set between light source module and the micro-fluidic chip, the polarizer set gradually including front and back
Wave plate and polarizer polarizing film；
The analyzer is set between the micro-fluidic chip and the image-forming module, the inspection set gradually including front and back
Inclined device polarizing film and analyzer wave plate；
The monochromatic light that the light supply apparatus issues passes sequentially through polarizer wave plate, polarizer polarizing film, on micro-fluidic chip
Sample channel, image-forming module described in analyzer polarizing film and analyzer wave plate directive.
Further, the monochromatic light that the light supply apparatus issues passes through the sample on the micro-fluidic chip with vertical angle
Channel.
Further, the polarizer and analyzer carry out spinning movement under the action of stepper motor.
Further, the control module includes single-chip microcontroller, and the control pin of the single-chip microcontroller is separately connected the light source
The light source switch of module, the stepper motor of detection module, the camera switch of image processing module and database module.
Further, described image processing module carries out polarization characteristic analysis and texture to the microalgae image information taken
Signature analysis, to judge the activity of cell.
The ballast water activity of microalgae detection method based on above-mentioned detection device that the present invention also provides a kind of, feature exist
In step includes:
1) device is installed, and according to requiring to be combined required each module installation, guarantee optical path can be according to set
Route passes through sample and is irradiated to the camera imaging in image processing module, prepares microalgae sample；
2) micro-fluidic chip is placed to objective table by opening device, and microalgae sample is added dropwise in the sample cell into micro-fluidic chip
Product, while opening the switch of light source, camera open stepping when sample image of the cameras capture into micro-fluidic chip
The switch of motor rotates the polarizer and analyzer automatically, by the way that the state of the polarizer and analyzer is arranged, obtains different polarization
Microalgae cell image under state with microalgae cell image of the rear camera shooting with polarization information and is sent to image procossing mould
Block is analyzed and processed；
3) image procossing handles the picture taken, inclined first with the incident light and emergent light that pass through sample
Vibration state finds out the Mueller matrix image of microalgae cell image in sample, then carries out the Mueller matrix image into one
Step analysis obtains to judge that the active parameter of microalgae cell, the parameter include polarization difference DP, parameter A, parameter b, parameter
The Muller matrixes parameter such as tan2x finally shows microalgae cell Activity Results in display interface；
4) data store, and measured data are stored into database module, are united to image and image parameter
Meter, so that measured data are analyzed and retrieved.
Compared with the prior art, the invention has the following advantages that
1, the present invention can change the polarization characteristic for changing polarised light by the relative position of polarizing film and wave plate, thus right
Microalgae cell activity is detected, and is not required to carry out any processing to microalgae cell in entire detection process, be avoided with its elsewhere
Reason method causes to damage when handling microalgae cell to cell.
2, each functional module assembling of apparatus of the present invention is small in size, and all only needs control module to other in whole process
Functional module is operated, and operator, which only needs to control corresponding switch, can be completed operation, is had small in size, easy to operate
Advantage.
To sum up, apply the technical scheme of the present invention can effectively solve microalgae cell Activity determination in the prior art it is difficult,
Apparatus structure complexity and problem inconvenient for use.
The present invention can be widely popularized in fields such as ballast water cytoactive detections based on the above reasons.
Detailed description of the invention
In order to more clearly explain the embodiment of the invention or the technical proposal in the existing technology, to embodiment or will show below
There is attached drawing needed in technical description to do simply to introduce, it should be apparent that, the accompanying drawings in the following description is this hair
Bright some embodiments for those of ordinary skill in the art without any creative labor, can be with
It obtains other drawings based on these drawings.
Fig. 1 is schematic structural diagram of the device of the invention.
Fig. 2 is control module schematic diagram of internal structure of the present invention.
Fig. 3 is detection module structural schematic diagram of the present invention.
Fig. 4 is microfluidic chip structure schematic diagram.
1, image-forming module；2, detection module；3, image processing module；4, database module；5, control module；6, monolithic
Machine；7, light source switch；8, light source module；9, stepper motor；10-1, polarizer wave plate；10-2, analyzer wave plate；11-1, it is polarized
Device polarizing film；11-2 analyzer polarizing film；12, camera switchs；13, camera；14, the polarizer；15, micro-fluidic chip master
Body；16, analyzer；17, glass slide；18, sample cell；19, sample channel；20, waste liquid tank.
Specific embodiment
It should be noted that in the absence of conflict, the feature in embodiment and embodiment in the present invention can phase
Mutually combination.The present invention will be described in detail below with reference to the accompanying drawings and embodiments.
In order to make the object, technical scheme and advantages of the embodiment of the invention clearer, below in conjunction with the embodiment of the present invention
In attached drawing, technical scheme in the embodiment of the invention is clearly and completely described, it is clear that described embodiment is only
It is only a part of the embodiment of the present invention, instead of all the embodiments.It is real to the description of at least one exemplary embodiment below
It is merely illustrative on border, never as to the present invention and its application or any restrictions used.Based on the reality in the present invention
Example is applied, every other embodiment obtained by those of ordinary skill in the art without making creative efforts all belongs to
In the scope of protection of the invention.
It should be noted that term used herein above is merely to describe specific embodiment, and be not intended to restricted root
According to exemplary embodiments of the present invention.As used herein, unless the context clearly indicates otherwise, otherwise singular
Also it is intended to include plural form, additionally, it should be understood that, when in the present specification using term "comprising" and/or " packet
Include " when, indicate existing characteristics, step, operation, device, component and/or their combination.
Unless specifically stated otherwise, positioned opposite, the digital table of the component and step that otherwise illustrate in these embodiments
It is not limited the scope of the invention up to formula and numerical value.Simultaneously, it should be clear that for ease of description, each portion shown in attached drawing
The size divided not is to draw according to actual proportionate relationship.Technology known for person of ordinary skill in the relevant, side
Method and equipment may be not discussed in detail, but in the appropriate case, and the technology, method and apparatus should be considered as authorizing explanation
A part of book.In shown here and discussion all examples, appointing should be construed as merely illustratively to occurrence, and
Not by way of limitation.Therefore, the other examples of exemplary embodiment can have different values.It should also be noted that similar label
Similar terms are indicated in following attached drawing with letter, therefore, once it is defined in a certain Xiang Yi attached drawing, then subsequent attached
It does not need that it is further discussed in figure.
As shown in Figs 1-4, the present invention provides a kind of polarization optofluidic piece ballast water activity of microalgae detection device, features
It is, comprising: image-forming module 1, detection module 2, image processing module 3, database module 4, control module 5, and provide single
The light source module 8 of coloured light light source.
The detection module 2 changes irradiation by the polarizer 14 and 16 relative rotation of analyzer and is micro-fluidic chip
The direction of vibration of light on the microalgae sample of upper flowing, to obtain image of the microalgae cell under different polarization states comprising
The polarizer, analyzer and micro-fluidic chip.
The micro-fluidic chip includes setting gradually micro-fluidic chip body 15 and glass slide 17, the miniflow from top to bottom
Control chip body fovea superior is carved with the sample channel 19 that sample cell 18 and waste liquid tank 20 is respectively set in a head and the tail, the micro-fluidic core
Piece can control the flow velocity of microalgae cell sample, consequently facilitating shooting to microalgae cell image；
The polarizer 14 is set between light source module and the micro-fluidic chip, is polarized including what front and back was set gradually
Device wave plate 10-1 and polarizer polarizing film 11-1；
The analyzer 16 is set between the micro-fluidic chip and the image-forming module, is set gradually including front and back
Analyzer polarizing film 10-2 and analyzer wave plate 11-2；
The monochromatic light that the light supply apparatus issues passes sequentially through polarizer wave plate 10-1, polarizer polarizing film 11-1, miniflow
Image-forming module 1 described in sample channel 19, analyzer polarizing film 11-2 and analyzer wave plate 10-2 directive on control chip.Light source dress
It sets on the monochromatic light vertical irradiation to the sample channel of micro-fluidic chip 15 of sending, while inclined with the step motor control polarizer
The angle of vibration piece and polarizing film and wave plate in wave plate and analyzer.
Realize that microalgae is thin after being set to the detection module 2, using the imaging lens group being made of two plano-convex lens
The image-forming module 1 of born of the same parents' image amplification；
It is obtained using camera 13 and is carried out through the image-forming module 1 amplification, the microalgae cell image under different polarization states
Processing is acquired with polarization information, and the image processing module 3 that all testing results are shown in display end；
It will test result and stored database module 4 to be retrieved and to be analyzed to data at any time；
And respectively with the light source module 8, image-forming module 1, detection module 2, image processing module 3 and database mould
The control module 5 that block 4 is connected, control modules act；
The monochromatic source that the light source module 8 provides is radiated at by detection module 2 with image-forming module 1 according to predetermined optical path
On the camera of image processing module 3, while control module 5 passes through the polarizing film and wave plate of the polarizer in control detection module 2
With the angle of polarizing film and wave plate in analyzer, camera 13 is made to capture the microalgae cell image under different polarization states, and
Polarization characteristic analysis and analysis of texture is carried out by described image processing module 3 to obtain micro- to judge the activity of cell
Algae cell activity result is for showing and storing.
Control module includes single-chip microcontroller, and the light source that the control pin of the single-chip microcontroller is separately connected the light source module is opened
Pass, the camera switch of the stepper motor of detection module, image processing module and database module.
The ballast water activity of microalgae detection method based on above-mentioned detection device that the present invention also provides a kind of, feature exist
In step includes:
1) device is installed, and according to requiring to be combined required each module installation, guarantee optical path can be according to set
Route passes through sample and is irradiated to the camera imaging in image processing module, prepares microalgae sample；
2) micro-fluidic chip is placed to objective table by opening device, and microalgae sample is added dropwise in the sample cell into micro-fluidic chip
Product, while opening the switch of light source, camera open stepping when sample image of the cameras capture into micro-fluidic chip
The switch of motor rotates the polarizer and analyzer automatically, by the way that the state of the polarizer and analyzer is arranged, obtains different polarization
Microalgae cell image under state with microalgae cell image of the rear camera shooting with polarization information and is sent to image procossing mould
Block is analyzed and processed；
3) image procossing handles the picture taken, inclined first with the incident light and emergent light that pass through sample
Vibration state finds out the Mueller matrix image of microalgae cell image in sample, then carries out the Mueller matrix image into one
Step analysis obtains to judge that the active parameter of microalgae cell, the parameter include polarization difference DP, parameter A, parameter b, parameter
The Muller matrixes parameter such as tan2x finally shows microalgae cell Activity Results in display interface；
4) data store, and measured data are stored into database module, are united to image and image parameter
Meter, so that measured data are analyzed and retrieved.
Below by specific embodiment, technical scheme is described further:
A kind of ballast hydroplankton on-line measuring device, which is characterized in that device includes: image-forming module 1, detection mould
Block 2, image processing module 3, database module 4, control module 5 and monochromatic source 8.
The control module 5 is connected with other modules, mainly includes single-chip microcontroller 6 and stepper motor 9, and single-chip microcontroller 6 can be controlled
The light source 8 being formed as in module, the opening and closing of the camera 13 in image processing module 3, stepper motor 9 are for controlling
The rotation angle of inclined device 14 and analyzer 16.
Described image processing module 3 is connected with detection module 2, is got using camera 13 with different polarization information
Microalgae image carries out processing and acquires with polarization information, and all testing results are shown in display end.
The database module 4 can will test result and be stored, convenient for being retrieved and being analyzed to data at any time.
The present embodiment is by taking activity of microalgae detection in ballast water as an example.Device is by image-forming module 1, detection module 2, image procossing
Module 3, database module 4, control module 5 form.Image-forming module 1 includes that light source 8 and lens group form, detection module include by
The polarizer 14 and analyzer 16 that polarizing film 11 and wave plate 10 form, and be connected with image-forming module 1, in image processing module 3
Camera 13 is connected with detection module, and database module 5 and image processing algorithm are embodied in the form of program in single-chip microcontroller 6
Out, mainly comprising single-chip microcontroller 6 and light source switch 7 and camera switch 12, light source 8 issues monochromatic light and successively passes through control module 4
Lens group, the polarizer 14, micro-fluidic chip, analyzer 16 are crossed, and is finally imaged in camera 13.
Detection module 2 has used polarizing film and wave plate as the tool of detection activity of microalgae, and the microalgae of different activities passes through
The effect of different polarization state caused by the polarizer 14 and analyzer 16 is also different.
15 material of micro-fluidic chip is polymethyl methacrylate (PMMA) or glass, and micro-fluidic chip 15, which is equipped with, to drip
The sample cell 18 and waste liquid tank 20 of sample-adding product, can control the flow velocity of microalgae cell, consequently facilitating shooting to microalgae cell.
Control module 5 is using 6 control module of single-chip microcontroller switch and stepping single machine 9, and module switch is for controlling light source 8 and taking the photograph
As first 13 open and close, stepper motor 9 is used to control the angle of polarizing film 11 and wave plate 10, guarantees the polarizer and analyzer
It can complete 0,45,90,135, the rotation of left-handed circle, dextrorotation fillet degree.
Image processing module 3 takes the microalgae image with polarization information, and carries out polarization characteristic analysis according to image
And texture analysis, and compared by each microalgae cell with known activity feature, to judge the activity of cell, and aobvious
Show end display.
Microalgae cell device for detecting activity provided by the invention can determine the work of microalgae cell according to handled result
Property, device is by light source sending monochromatic light, and the polarizer in module changes the polarization properties of light after testing, is subsequently incident to micro-
In fluidic chip, after light is incident on the sample in micro-fluidic chip internal channel, the polarization properties of light can change, therefore after
It is continuous to be emitted in analyzer, and be finally imaged in camera, since the angle change of the polarizer and analyzer can determine finally
Polarization image, it is therefore desirable to angle control is carried out to the polarizer and analyzer using stepper motor, the polarization obtained to shooting
Image carries out image algorithm processing, the processing of processing and polarization texture features including polarization parameter, finally according to polarization parameter
Microalgae cell activity is detected with texture features parameter, measured result will be stored into database module.
The another aspect of the present embodiment additionally provides the inspection of the polarization optofluidic piece ballast water activity of microalgae based on above-mentioned apparatus
Survey method, comprising:
1) device is installed, and according to requiring to be combined required various modules installation, guarantee optical path can be according to set
Route by sample and being irradiated to the camera imaging in processing module.Microalgae sample is prepared, and makes micro-fluidic chip.
2) micro-fluidic chip is placed to objective table by opening device, and microalgae sample is added dropwise in the sample cell into micro-fluidic chip
Product, while opening the switch of light source, camera when the sample to be seen into chip, open the switch of stepper motor, make its from
It is dynamic that the polarizer and analyzer are rotated, by the way that the state of the polarizer and analyzer is arranged, obtain under different polarization state
Microalgae cell image is handled with microalgae cell image of the rear camera shooting with polarization information and in processing module.
Specifically, opening the switch of light source and camera when to detect to microalgae cell, camera can be observed
To light, sample, which is then added, flows through sample in entire channel, steady in channel interior to microalgae cell
Periodically, stepper motor can be made to work, stepper motor controls the polarizer and analyzer, and the rotation of polarizing film is arranged
Angle is 0 °, 45 °, 90 °, and 135 ° of four kinds of angles, and left-right rotary circularly polarized light is set, in this way, the polarizer and analyzer all have 6
Kind polarization state, obtains 36 kinds of different polarization state images eventually by camera imaging, when image taking finishes, storage is arrived
It is handled in single-chip microcontroller, the polarizer and analyzer is finally recalled into home position, then the switch of light source and camera is closed.
3) image procossing handles the picture taken, inclined first with the incident light and emergent light that pass through sample
Vibration state finds out the Mueller matrix image of sample, then by the image carry out further algorithm analyze to obtain can judge it is micro-
The parameter of algae cell activity, the parameter include the Muller matrixes parameters such as polarization difference DP, parameter A, parameter b, parameter tan2x, most
Microalgae cell Activity Results are shown in display interface afterwards.
Specifically, when get above-mentioned shooting to polarization image after, need to analyze these images, algorithm uses
The algorithm of Muller matrix calculates each of Muller matrix matrix element image according to different images, includes big in image
The polarization information of amount, therefore the polarization parameter figure of the characterization microalgae cell such as degree of polarization and polarization difference that can find out Muller matrix
Picture, while matrix decomposition parameter, the analysis and calculating of frequency domain characteristic can also be carried out to it.According to resulting polarization parameter image
Texture analysis is carried out, the statistical parameter of image internal feature is found by gray level co-occurrence matrixes, is distinguished according to the difference of its parameter
The microalgae cell of other different activities.Finally obtained polarization image, statistical parameter and microalgae cell activity identification result are existed
It is shown in the display end of single-chip microcontroller.
4) data store, and measured data are stored in the single-chip microcontroller into control module, join to image and image
Number is counted, convenient for measured data are analyzed and retrieved.
The detection device that the present invention is handled by a kind of cellular polarization characteristic can carry out the cell in ballast water for ship
Cytoactive detection judges the activity of different cells, carries out lossless decomposition to microalgae cell in whole process, while being examined
It is small to survey device volume, and user of service need to only be switched on cell activity inspection can be realized with closed procedure when detecting
It surveys, whole process is to automatically process, and operates very simple.
Finally, it should be noted that the above embodiments are only used to illustrate the technical solution of the present invention., rather than its limitations；To the greatest extent
Pipe present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that: its according to
So be possible to modify the technical solutions described in the foregoing embodiments, or to some or all of the technical features into
Row equivalent replacement；And these are modified or replaceed, various embodiments of the present invention technology that it does not separate the essence of the corresponding technical solution
The range of scheme.
Claims (7)
1. a kind of polarization optofluidic piece ballast water activity of microalgae detection device characterized by comprising
The light source module of monochromatic light source is provided；
By constitute the polarizer polarizing film and quarter wave plate and constitute analyzer polarizing film and quarter wave plate relative rotation thus
The direction of vibration for changing the light that irradiation is on the microalgae sample flowed on micro-fluidic chip, to obtain microalgae cell in difference
The detection module of image under polarization state；
Realize that microalgae cell image is put after being set to the detection module, using the imaging lens group being made of two convex lenses
Big image-forming module；
Amplified using camera acquisition through the image-forming module, the microalgae cell image under different polarization states carries out processing and inclined
Shake information collection, and the image processing module that all testing results are shown in display end；
It will test result and stored database module to be retrieved and to be analyzed to data at any time；
And be connected respectively with the light source module, image-forming module, detection module, image processing module and database module,
Control the control module of modules movement；
The monochromatic source that the light source module provides according to predetermined optical path be light source, the polarizer, micro-fluidic chip, analyzer, at
Camera shooting as being radiated at image processing module on module vertical irradiation to micro-fluidic chip and by detection module and image-forming module
On head, while control module captures camera by the relative rotary motion of the polarizer and analyzer in control detection module
Microalgae cell image under to different polarization states, and be analyzed and processed to obtain microalgae cell work by described image processing module
Property result for showing and store.
2. detection device according to claim 1, which is characterized in that the detection module include the polarizer, analyzer with
And micro-fluidic chip；
The micro-fluidic chip includes setting gradually micro-fluidic chip body and glass slide, the micro-fluidic chip master from top to bottom
Body fovea superior is carved with the sample channel that sample cell and waste liquid tank is respectively set in a head and the tail, and the micro-fluidic chip is due to gravity factor
The flow velocity of microalgae cell sample can be controlled, consequently facilitating shooting to microalgae cell image；
The polarizer is set between light source module and the micro-fluidic chip, the polarizer wave plate set gradually including front and back
And polarizer polarizing film；
The analyzer is set between the micro-fluidic chip and the image-forming module, the analyzer set gradually including front and back
Polarizing film and analyzer wave plate；
The monochromatic light that the light supply apparatus issues passes sequentially through polarizer wave plate, polarizer polarizing film, the sample on micro-fluidic chip
Image-forming module described in product channel, analyzer polarizing film and analyzer wave plate directive.
3. detection device according to claim 2, which is characterized in that the monochromatic light vertical irradiation that light source issues is to micro-fluidic
On the sample channel of chip.
4. detection device according to claim 2, which is characterized in that the work of the polarizer and analyzer in stepper motor
Spinning movement is carried out under.
5. detection device according to claim 1, which is characterized in that the control module includes single-chip microcontroller, the monolithic
The control pin of machine is separately connected the light source switch of the light source module, the stepper motor of detection module, image processing module
Camera switch and database module.
6. detection device according to claim 1, which is characterized in that described image processing module is to the microalgae figure taken
As information progress polarization characteristic analysis and analysis of texture, to judge the activity of cell.
7. a kind of ballast water activity of microalgae detection method based on detection device described in any one of claim 1-6, special
Sign is that step includes:
1) device is installed, and according to requiring to be combined required each module installation, guarantee optical path can be according to set route
Pass through sample and be irradiated to the camera imaging in image processing module, prepares microalgae sample；
2) micro-fluidic chip is placed to objective table by opening device, and microalgae sample is added dropwise in the sample cell into micro-fluidic chip, together
The switch of Shi Kaiqi light source, camera opens stepper motor when sample image of the cameras capture into micro-fluidic chip
Switch, rotates the polarizer and analyzer automatically, by the way that the state of the polarizer and analyzer is arranged, obtains under different polarization state
Microalgae cell image, with rear camera shooting with polarization information microalgae cell image and send to image processing module carry out
Analysis processing；
3) image procossing handles the picture taken, first with the incident light and outgoing light polarization shape by sample
State finds out the Mueller matrix image of microalgae cell image in sample, then is further divided the Mueller matrix image
Analysis obtains to judge that the active parameter of microalgae cell, the parameter include polarization difference DP, parameter A, parameter b, parameter tan2x etc.
Muller matrix parameter finally shows microalgae cell Activity Results in display interface；
4) data store, and measured data are stored into database module, are counted to image and image parameter, with
Just measured data are analyzed and is retrieved.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811333637.7A CN109187367B (en) | 2018-11-09 | 2018-11-09 | Device and method for detecting activity of microalgae in ballast water by using polarized light flow control sheet |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811333637.7A CN109187367B (en) | 2018-11-09 | 2018-11-09 | Device and method for detecting activity of microalgae in ballast water by using polarized light flow control sheet |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN109187367A true CN109187367A (en) | 2019-01-11 |
| CN109187367B CN109187367B (en) | 2021-05-14 |
Family
ID=64939004
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811333637.7A Active CN109187367B (en) | 2018-11-09 | 2018-11-09 | Device and method for detecting activity of microalgae in ballast water by using polarized light flow control sheet |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109187367B (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114674760A (en) * | 2022-05-30 | 2022-06-28 | 中国科学院海洋研究所 | Symmetric marine planktonic algae polarization scattering multi-angle measuring instrument and measuring method thereof |
| CN115290572A (en) * | 2022-10-08 | 2022-11-04 | 长春理工大学 | Red tide polarization monitoring device based on active illumination and monitoring method thereof |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN202421056U (en) * | 2012-01-11 | 2012-09-05 | 水利部中国科学院水工程生态研究所 | Rapid plankton monitoring device |
| WO2016071356A1 (en) * | 2014-11-05 | 2016-05-12 | Medico-Chemical Lab. Aps | A ballast water analysis system |
| CN107101943A (en) * | 2017-05-18 | 2017-08-29 | 大连海事大学 | A kind of optofluidic is without lens holographic imaging activity of microalgae detection means and method |
| CN107688635A (en) * | 2017-08-22 | 2018-02-13 | 清华大学深圳研究生院 | Alga Identify Method based on backward polarization scattering and the device applied to this method |
| CN107957397A (en) * | 2017-11-22 | 2018-04-24 | 大连海事大学 | A kind of microalgae classification and Detection device and detection method based on hologram image feature |
-
2018
- 2018-11-09 CN CN201811333637.7A patent/CN109187367B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN202421056U (en) * | 2012-01-11 | 2012-09-05 | 水利部中国科学院水工程生态研究所 | Rapid plankton monitoring device |
| WO2016071356A1 (en) * | 2014-11-05 | 2016-05-12 | Medico-Chemical Lab. Aps | A ballast water analysis system |
| CN107101943A (en) * | 2017-05-18 | 2017-08-29 | 大连海事大学 | A kind of optofluidic is without lens holographic imaging activity of microalgae detection means and method |
| CN107688635A (en) * | 2017-08-22 | 2018-02-13 | 清华大学深圳研究生院 | Alga Identify Method based on backward polarization scattering and the device applied to this method |
| CN107957397A (en) * | 2017-11-22 | 2018-04-24 | 大连海事大学 | A kind of microalgae classification and Detection device and detection method based on hologram image feature |
Non-Patent Citations (1)
| Title |
|---|
| 孟雄飞等: "《船舶压载水中微藻快速检测系统设计》", 《仪表技术与传感器》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114674760A (en) * | 2022-05-30 | 2022-06-28 | 中国科学院海洋研究所 | Symmetric marine planktonic algae polarization scattering multi-angle measuring instrument and measuring method thereof |
| CN115290572A (en) * | 2022-10-08 | 2022-11-04 | 长春理工大学 | Red tide polarization monitoring device based on active illumination and monitoring method thereof |
| CN115290572B (en) * | 2022-10-08 | 2023-01-10 | 长春理工大学 | Red tide polarization monitoring device based on active illumination and monitoring method thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN109187367B (en) | 2021-05-14 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US11125675B2 (en) | Fluid suspended particle classifier | |
| CN107101943B (en) | Device and method for detecting microalgae activity by optical flow control lens-free holographic imaging | |
| CN109145830A (en) | A kind of intelligence water gauge recognition methods | |
| CN107688635B (en) | Based on the rear Alga Identify Method to polarization scattering and the device applied to this method | |
| JPH0352573B2 (en) | ||
| Martsenyuk et al. | Multispectral control of water bodies for biological diversity with the index of phytoplankton | |
| WO2016117460A1 (en) | Water quality inspection system | |
| CN109187367A (en) | A kind of polarization optofluidic piece ballast water activity of microalgae detection device and method | |
| CN110334691B (en) | Microorganism water quality monitoring method, system and storage medium | |
| CN110487223A (en) | A kind of micro- plastics detection device and method based on spatial correlation Yu phase difference value product | |
| Luo et al. | Automatic Identification of Diatoms with Circular Shape using Texture Analysis. | |
| Owen et al. | Reporting of methods for automated devices: A systematic review and recommendation for studies using FlowCam for phytoplankton | |
| CN107533217A (en) | Method and apparatus for microtechnic | |
| Le et al. | Benchmarking and automating the image recognition capability of an in situ plankton imaging system | |
| CN114813492A (en) | Microscopic Raman in-situ measurement system for underwater micro-plastic | |
| CN108318424A (en) | A kind of underwater planktonic organism automatic imaging device and imaging method | |
| CN108827891A (en) | Ballast water for ship microalgae cell biology amount detection systems and method | |
| Vaughan et al. | A review of microscopic cell imaging and neural network recognition for synergistic cyanobacteria identification and enumeration | |
| CN106442488B (en) | method for detecting living organisms with size of more than 50 mu m in ballast water | |
| Varghese et al. | Collection and estimation of zooplankton | |
| US20040109586A1 (en) | Shadowed image particle profiling and evaluation recorder | |
| KR20100034717A (en) | System and method for processing microorganism of ballast water on the ship | |
| RU2303812C2 (en) | Method for identifying and calculating cells in human and animal biological media and device for its implementation | |
| EP3891560A1 (en) | Portable uv holographic microscope for high-contrast protein crystal imaging | |
| KR100558613B1 (en) | Method for measuring cells of plankton using digital image processing method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |