CN108318424A - A kind of underwater planktonic organism automatic imaging device and imaging method - Google Patents
A kind of underwater planktonic organism automatic imaging device and imaging method Download PDFInfo
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- CN108318424A CN108318424A CN201810311268.5A CN201810311268A CN108318424A CN 108318424 A CN108318424 A CN 108318424A CN 201810311268 A CN201810311268 A CN 201810311268A CN 108318424 A CN108318424 A CN 108318424A
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- 238000003384 imaging method Methods 0.000 title claims abstract description 36
- 230000000007 visual effect Effects 0.000 claims abstract description 21
- 239000012530 fluid Substances 0.000 claims abstract description 14
- 239000002245 particle Substances 0.000 claims description 9
- 239000003242 anti bacterial agent Substances 0.000 claims description 7
- 238000001914 filtration Methods 0.000 claims description 6
- 239000006260 foam Substances 0.000 claims description 5
- 238000000034 method Methods 0.000 claims description 5
- 239000010865 sewage Substances 0.000 claims description 5
- 230000005540 biological transmission Effects 0.000 claims description 3
- 239000000463 material Substances 0.000 claims description 3
- 238000005086 pumping Methods 0.000 claims description 3
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 claims description 2
- 239000004677 Nylon Substances 0.000 claims description 2
- 229910052802 copper Inorganic materials 0.000 claims description 2
- 239000010949 copper Substances 0.000 claims description 2
- 229920001778 nylon Polymers 0.000 claims description 2
- 239000013535 sea water Substances 0.000 abstract description 2
- 238000010586 diagram Methods 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- 238000001514 detection method Methods 0.000 description 2
- 239000003599 detergent Substances 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 230000007774 longterm Effects 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 241000902900 cellular organisms Species 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 230000005611 electricity Effects 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 238000007667 floating Methods 0.000 description 1
- 238000011065 in-situ storage Methods 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 239000002068 microbial inoculum Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 230000001629 suppression Effects 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/34—Purifying; Cleaning
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/84—Systems specially adapted for particular applications
- G01N21/85—Investigating moving fluids or granular solids
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses a kind of underwater planktonic organism automatic imaging device and imaging method, which includes fluid modules, image-forming module and control module etc.；Fluid modules include sample pump, the first container, second container etc.；Sample pump includes syringe, distributing valve and control system；The entrance of syringe is connected with the first entrance of distributing valve, second entrance is as thief hatch, third entrance is connected with the first entrance of the first container, the first container is connected with second container by flow cell, and the outlet of second container is sequentially communicated first filter, miniature sheath stream pump, the second filter and the first container；In the visual field of image-forming module, control system, miniature sheath stream pump and image-forming module are electrically connected with control module flow cell.The present invention provides a kind of can be automatically performed under seawater and is taken pictures to underwater planktonic organism and to the device that data are handled for a long time, to observe underwater planktonic organism for a long time and providing solution to harmful microbe early warning.
Description
Technical field
The present invention relates to planktonic organism sample detecting technical field more particularly to a kind of underwater planktonic organism automated imaging dresses
It sets and imaging method.
Background technology
Halomereid occupies extremely important position, quantity variation and spatial distribution in marine ecosystems
Marine ecosystems, even Global climate change can all be had an important influence on.
Currently, have existed some halomereid analytical equipments for being related to imaging, but more lay particular emphasis on fluorescence etc.
Analysis that characteristic information is combined, to whole planktonic organism, can not ensure not omit, or can only be right in imaging process
Some specific biotas are imaged, and cannot even ensure that some of planktonic organism individuals by complete imaging.In addition, tradition is floating
Trip biological detection imaging method needs first sample planktonic organism, are preserved, and then take back laboratory and are analyzed, herein mistake
Different degrees of damage can be inevitably caused in journey to sample, or causes some planktonic organisms that can not be detected.Therefore, above-mentioned to adopt
Sample detection process all inevitably has omission problem.
Invention content
The present invention overcomes the disadvantages of the prior art, it is therefore intended that Biodiversity of Plankton in seawater ecology is recognized in solution
Deficiency in knowledge provides a kind of underwater planktonic organism automatic imaging device and imaging method, swims under water life for long-term observation
Object and solution is provided to harmful microbe early warning.
In order to solve the above-mentioned technical problem, what the invention is realized by the following technical scheme：A kind of underwater planktonic organism from
Dynamic imaging device, including sealed compartment and fluid modules, image-forming module and control module in sealed compartment；
The fluid modules include sample pump, the first container, flow cell, second container, miniature sheath stream pump, the first filtering
Device, the second filter；The sample pump includes syringe, distributing valve and control system, and syringe and distributing valve are with control
System is connected, and the entrance of syringe is connected with the first entrance of distributing valve, the second entrance of distributing valve as thief hatch,
The third entrance of distributing valve is connected with the first entrance of the first container, and the first container is fixedly connected with second container, described
Second container is transparent vessel, and the first container is connected with second container by flow cell, and flow cell is arranged in second container,
The outlet of second container is sequentially communicated first filter, miniature sheath stream pump and the second filter, the outlet of the second filter and the
The entrance of one container is connected；For flow cell in the visual field of image-forming module, control system, miniature sheath stream pump and image-forming module are equal
It is electrically connected with control module.
Further, the image-forming module includes flash lamp, condenser, visual field grating and CCD camera, visual field grating and
Thread road in light path and flow cell between CCD camera is perpendicular, and flash lamp passes sequentially through condenser, visual field grating to circulation
Thin runner section in pond is exposed, and camera is entered after exposure；Flash lamp and CCD camera are electrically connected with control module.
Further, with the filter screen being made of copper mesh and nylon wire outside the thief hatch.
Further, the 4th entrance of the distributing valve is connected with standard particle container.
Further, the 5th entrance of the distributing valve is connected with row's foam mouth of the first container, and the 6th of distributing valve the
Entrance is as sewage draining exit.
Further, the 7th entrance of the distributing valve is connected with antibacterial agent container, the 8th entrance of distributing valve
It is connected with antibacterial agent container.
Further, the sealed compartment is watertight structure, can carry out data with the external world respectively by data line and supply lines
Transmission and electrical energy transportation；The control module is connect with supply lines and data line, can be powered by supply lines and be passed through data line
Periodically to laboratory terminal returned data.
Further, the outlet of second filter is divided into two-way, and two-way is connected with the first container, and two-way is symmetrical
It is arranged in the both sides of the first entrance of the first container.
Further, the second container is made of light-sensitive material.
The present invention also provides a kind of underwater planktonic organism automatic imaging methods, and this method comprises the following steps：
Step (1) starts miniature sheath stream pump, keeps pipeline interior uniform without particle sheath stream flow velocity, and bubble-free in flow cell；
Step (2) opens the first entrance of distributing valve and the second entrance, will include planktonic organism by syringe
The solution of sample cell is drawn by the first entrance in syringe, and the second entrance is closed, and is opened third entrance, is being controlled
The piston of syringe gradually pushes under the control of system processed, and the solution in syringe is injected into the first appearance by third entrance
In device, surrounding sheath stream make phytoplankton cells sample focus on flow cell thread road middle section and arrange flow through one by one；
Step (3) adjustment image-forming module makes it focus on flow cell central part, to the cell sample of wherein fluid focus
High speed imaging is carried out, imaging frequency need to be identical as the frequency that sample flows through visual field to guarantee to capture all samples and not have
There is the part being missed because sample cell part is beyond visual field；
Step (4) can reopen the first entrance after the completion of the piston of syringe drops to all step numbers and go out with second
Entrance sucks sample again, carries out circulate operation.
Step (5) by the solution after flow cell under miniature sheath stream pumping action, pass sequentially through the first container, sheath stream pump and
It after the filtering of second container, then flows back in the first container, forms sheath stream.
Compared with prior art, the beneficial effects of the invention are as follows：
(1) through the above technical solution, field observation can be carried out under water and is corrected and safeguards automatically, realize length
The acquisition of phase original position data avoids the influence and damage brought to sample in sampling, transportational process.
(2) it can be achieved to include that phytoplankton carries out with zooplankter to the planktonic organism of any kind or planktonic organism group
Imaging, to sample, there is no limit without the pretreatment for carrying out complexity.
(3) it can be achieved completely to be imaged the planktonic organism of arbitrary size or planktonic organism group, and eliminate
For the issuable omission phenomenon of certain rare planktonic organisms, plankton community structure can be appreciated more fully.
(4) long-term sample in situ can be carried out to water field to collect, can notice objective area biocoene in early days
Minor change, the early warning of early stage is realized to the disaster of planktonic organism.
Description of the drawings
Specific embodiments of the present invention will be described in further detail below in conjunction with the accompanying drawings
Fig. 1 is the system schematic of the present invention；
Fig. 2 is the fluid system configuration schematic diagram of the present invention；
Fig. 3 is inventive samples pump portion enlarged drawing；
Fig. 4 is the image-forming module structural schematic diagram of the present invention；
Fig. 5 is the operational flow diagram of the present invention；
In figure：Sealed compartment 1, fluid modules 2, image-forming module 3, control module 4, first filter 5, the second filter 6, sample
Product pump 7, flow cell 9, second container 10, miniature sheath stream pump 11, syringe 12, distributing valve 13, sewage draining exit 14, is adopted at the first container 8
Sample mouth 15, standard particle container 16, antibacterial agent container 17, detergent container 18, flash lamp 19, condenser 20, visual field grating 21,
CCD camera 22.
Specific implementation mode
The specific implementation mode of the present invention is described further below in conjunction with the accompanying drawings.
As shown in Figs 1-4, a kind of underwater planktonic organism automatic imaging device, which is characterized in that including sealed compartment 1 and peace
Fluid modules 2, image-forming module 3 in sealed compartment 1 and control module 4；
The sealed compartment 1 is watertight structure, can be carried out data transmission respectively with the external world by data line and supply lines and electricity
It can conveying.The control module 4 is connect with supply lines and data line, can be powered by supply lines and by data line periodically to reality
Test room terminal returned data.
The fluid modules 2 include first filter 5, the second filter 6, sample pump 7, the first container 8, flow cell 9, the
Two containers 10, miniature sheath stream pump 11；Sample pump 7 includes syringe 12, distributing valve 13 and control system, syringe 12 and distributing valve
13 are connected with control system, and Kloehn, the Versapump V6 of Inc. may be used in 12 sample of syringe pump 7 in the present embodiment
Syringe pump, but not limited to this；The entrance of syringe 12 is connected with the first entrance of distributing valve 13, and the second of distributing valve 13
Entrance is as thief hatch 15, for conveying sample；The third entrance of distributing valve 13 is connected with the first entrance of the first container 8
Logical, the 4th entrance of the distributing valve 13 is connected with standard particle container 16, the 5th entrance of the distributing valve 13 and
Row's foam mouth of the first container 8 is connected, for excluding foam；6th entrance of distributing valve 13 is as sewage draining exit 14, for arranging
Go out waste liquid；7th entrance of the distributing valve 13 is connected with antibacterial agent container 17, the 8th entrance and the suppression of distributing valve 13
Microbial inoculum container 17 is connected；The first container 8 is fixedly connected with second container 10, and the second container 10 is transparent vessel, described
Second container 10 is made of light-sensitive material.The first container 8 is connected with second container 10 by flow cell 9, and the setting of flow cell 9 exists
In second container 10, the outlet of second container 10 is sequentially communicated first filter 5, miniature sheath stream pump 11 and the second filter 6, institute
The outlet for stating the second filter 6 is divided into two-way, and two-way is connected with the first container 8, and two-way is arranged symmetrically in the first container 8
The both sides of first entrance.
The image-forming module 3 includes flash lamp 19, condenser 20, visual field grating 21, CCD camera 22, and the setting of flow cell 9 exists
In light path between visual field grating 21 and CCD camera 22, in the light path and flow cell 9 between visual field grating 21 and CCD camera 22
Thread road it is perpendicular, so that flash lamp 19 is passed sequentially through condenser 20, visual field grating 21 to the thin flow path portion in flow cell 9
Divide and be exposed, into camera 22；Flash lamp 19 and CCD camera 22 are electrically connected with control module 4.
The product of the PCM-3365 models of Yan Hua companies may be used in control module 4, but not limited to this.
As shown in figure 5, the course of work of the present invention is as follows：
(1) start miniature sheath stream pump 11, keep pipeline interior uniform without particle sheath stream flow velocity, and bubble-free in flow cell 9, if the
Sewage draining exit can be passed through again by arranging the 5th entrance suction of foam mouth and distributing valve 13 by having a large amount of bubbles cannot exclude in one container 8
14 exclude；
(2) the first entrance of distributing valve 13 and the second entrance are opened, will will include planktonic organism by syringe 12
The solution of sample cell is drawn by the first entrance in syringe 12, and the second entrance is closed, and opens third entrance,
The piston of syringe gradually pushes under the control of control system, and the solution in syringe 12 is injected by third entrance
In one container 8, surrounding sheath stream make phytoplankton cells sample focus on flow cell 9 thread road middle section and arrange flow one by one
It crosses, avoids the overlapping for deviateing the focus point and cell sample of camera 22；
(3) light source that adjustment image-forming module 3 makes flash lamp 19 provide passes through condenser 20 and 21 perpendicular projection of visual field grating
In the thin runner section of flow cell 9, into camera 22.22 focus point of camera is set to be located at 9 central part of flow cell, to realize to it
The cell sample of middle fluid focus carries out high speed imaging, and imaging frequency need to be identical as the frequency that sample flows through visual field to guarantee
The part for capturing all samples and not being missed because sample cell part is beyond visual field；The figure that image-forming module 3 obtains
As unified by data line transfer to terminal after the extraction of cell storage.
(4) the first entrance and second can be reopened after the completion of the piston of syringe 12 drops to all step numbers to come in and go out
Mouth sucks sample again.
(5) by the solution after flow cell under miniature sheath stream pumping action, the first container, sheath stream pump and second are passed sequentially through
It after the filtering of container, then flows back in the first container, forms sheath stream.
In addition, the fluid modules 2 can also carry out regular calibration and safeguard, can be controlled at regular intervals by control module 4
Sample preparation product pump 7, which by standard particle container 16 inputs normal size particle, to carry out imaging and is used as benchmark size and is corrected, and leads to
It crosses the antibacterial agent container 17 for being connected to distributing valve 13 and detergent container 18 clears up whole system runner.
Those skilled in the art will be clear that, with the development of technology, can realize in a number of different ways
The basic conception of the present invention.Therefore, the present invention and embodiment are not limited to example discussed above, but can be in claim
Variation in range.
Claims (10)
1. a kind of underwater planktonic organism automatic imaging device, which is characterized in that including sealed compartment and in sealed compartment
Fluid modules, image-forming module and control module etc.；The fluid modules include sample pump, the first container, flow cell, the second appearance
Device, miniature sheath stream pump, first filter, the second filter；The sample pump includes syringe, distributing valve and control system；Note
Emitter and distributing valve are connected with control system, and the entrance of syringe is connected with the first entrance of distributing valve, distributing valve
The second entrance as thief hatch, the third entrance of distributing valve is connected with the first entrance of the first container, the first container
It being fixedly connected with second container, the second container is transparent vessel, and the first container is connected with second container by flow cell,
Flow cell is arranged in second container, and the outlet of second container is sequentially communicated first filter, miniature sheath stream pump and the second filtering
Device, the outlet of the second filter are connected with the entrance of the first container；Flow cell in the visual field of image-forming module, control system,
Miniature sheath stream pump and image-forming module are electrically connected with control module.
2. a kind of underwater planktonic organism automatic imaging device according to claim 1, which is characterized in that the image-forming module
It is thin in the light path and flow cell between visual field grating and CCD camera including flash lamp, condenser, visual field grating and CCD camera
Runner is perpendicular, and flash lamp passes sequentially through condenser, visual field grating is exposed the thin runner section in flow cell, after exposure
Into camera；Flash lamp and CCD camera are electrically connected with control module.
3. a kind of underwater planktonic organism automatic imaging device according to claim 1, which is characterized in that outside the thief hatch
Portion carries the filter screen being made of copper mesh and nylon wire.
4. a kind of underwater planktonic organism automatic imaging device according to claim 1, which is characterized in that the distributing valve
4th entrance is connected with standard particle container.
5. a kind of underwater planktonic organism automatic imaging device according to claim 4, which is characterized in that the distributing valve
5th entrance is connected with row's foam mouth of the first container, and the 6th entrance of distributing valve is as sewage draining exit.
6. a kind of underwater planktonic organism automatic imaging device according to claim 5, which is characterized in that the distributing valve
7th entrance is connected with antibacterial agent container, and the 8th entrance of distributing valve is connected with antibacterial agent container.
7. a kind of underwater planktonic organism automatic imaging device according to claim 1, which is characterized in that the sealed compartment is
Watertight structure, can be carried out data transmission with the external world and electrical energy transportation respectively by data line and supply lines；The control module with
Supply lines is connected with data line, can be powered by supply lines and by data line periodically to laboratory terminal returned data.
8. a kind of underwater planktonic organism automatic imaging device according to claim 1, which is characterized in that second filtering
The outlet of device is divided into two-way, and two-way is connected with the first container, and two-way is arranged symmetrically in the two of the first entrance of the first container
Side.
9. a kind of underwater planktonic organism automatic imaging device according to claim 1, which is characterized in that the second container
It is made of light-sensitive material.
10. a kind of imaging method of underwater planktonic organism automatic imaging device according to claim 1, which is characterized in that
This method comprises the following steps：
Step (1) starts miniature sheath stream pump, keeps pipeline interior uniform without particle sheath stream flow velocity, and bubble-free in flow cell；
Step (2) opens the first entrance of distributing valve and the second entrance, will include planktonic organism sample by syringe
The solution of cell is drawn by the first entrance in syringe, and the second entrance is closed, and opens third entrance, is in control
The piston of syringe gradually pushes under the control of system, and the solution in syringe is injected into the first container by third entrance
In, surrounding sheath stream make phytoplankton cells sample focus on flow cell thread road middle section and arrange flow through one by one；
Step (3) adjustment image-forming module makes it focus on flow cell central part, is carried out to the cell sample of wherein fluid focus
High speed imaging, imaging frequency need to it is identical as the frequency that sample flows through visual field with guarantee to capture all samples and it is no because
The part that sample cell part is missed beyond visual field；
Step (4) can reopen the first entrance and the second entrance after the completion of the piston of syringe drops to all step numbers
Sample is sucked again, carries out circulate operation.
Step (5) under miniature sheath stream pumping action, passes sequentially through the first container, sheath stream pump and second by the solution after flow cell
It after the filtering of container, then flows back in the first container, forms sheath stream.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201810311268.5A CN108318424A (en) | 2018-04-09 | 2018-04-09 | A kind of underwater planktonic organism automatic imaging device and imaging method |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810311268.5A CN108318424A (en) | 2018-04-09 | 2018-04-09 | A kind of underwater planktonic organism automatic imaging device and imaging method |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108760046A (en) * | 2018-08-17 | 2018-11-06 | 上海大学 | A kind of luminous planktonic organism optically-captured device in deep-sea |
| CN110333236A (en) * | 2019-07-19 | 2019-10-15 | 中国海洋大学 | Planktonic organism multi-angle of view in situ detection device |
| WO2021218209A1 (en) * | 2020-04-29 | 2021-11-04 | 中国科学院深圳先进技术研究院 | Sampling apparatus and imaging device |
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| CN101900666A (en) * | 2009-06-05 | 2010-12-01 | 中国科学院海洋研究所 | Sample detecting circulating water device with planktonic animal optical counting function |
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Application publication date: 20180724 |