CN109182342A - 一种水稻抗稻瘟病基因Pisj及其应用 - Google Patents
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Abstract
本发明属于分子育种技术领域，公开了一个水稻抗稻瘟病基因Pisj及其应用。通过图位克隆技术从抗稻瘟病水稻品种“绥粳8号”获得抗稻瘟病基因Pisj，并筛选到了其共分离分子标记S6‑43‑32，该标记为显性标记；利用该共分离分子标记，对杂交后代的基因组DNA进行PCR扩增，扩增产物在1.5％的琼脂糖凝胶上电泳检测，获得的分子量为368bp的水稻植株，即为含水稻抗稻瘟病基因Pisj的材料，经稻瘟菌接种鉴定，这些材料表现出抗性。本发明所提供的水稻抗稻瘟病基因Pisj为水稻抗病育种提供了新的基因资源，其共分离分子标记操作简便，检测结果准确，能够快速筛选出含有Pisj基因的品种或品系用于育种，可大大加快水稻稻瘟病抗性品种的选育过程。
Description
技术领域
本发明属于分子育种技术领域，具体涉及一种水稻抗稻瘟病基因Pisj及其应用。
背景技术
稻瘟病是由丝状真菌稻瘟菌引起的水稻病害，几乎每年都会造成水稻大幅减产，有时甚至绝收。培育高抗水稻品种是防治稻瘟病最经济、最安全的方式之一。然而在农业生产过程中，稻瘟菌菌群处于不断变化之中。因此，一个新的水稻品种在推广3-5年后，其抗性会随着稻瘟菌群的变化而逐渐丧失。因此，挖掘广谱抗稻瘟病基因是水稻抗病育种的重要基础。
发明内容
针对以上技术问题，本发明提供了一种水稻抗稻瘟病基因Pisj及其应用，从水稻基因组内利用图位克隆的方法分离克隆到一个控制水稻稻瘟病抗性的基因，命名为Pisj，该抗性基因能够提高底盘品种的抗稻瘟病能力。利用该基因的共分离分子标记S6-43-32，能够在水稻选育中进行有目的的选择，为通过分子育种的方法选育抗稻瘟病水稻新品种提供新基因和指导依据。
发明人通过接菌鉴定发现“绥粳8号”对大多数稻瘟菌表现出抗病表型，利用图位克隆的方法解析了控制“绥粳8号”抗性的基因Pisj位于6号染色体短臂上的Piz基因座内。在回交群体中通过PCR检测和接种鉴定，确定含有Pisj基因的水稻植株，即杂合基因型或纯合Pisj基因型，均对稻瘟菌表现出抗病表型，而不含有Pisj基因的水稻植株，则均表现出感病表型。
本发明的技术方案为：一种水稻抗稻瘟病基因Pisj，所述水稻抗稻瘟病基因Pisj为显性基因，其核苷酸序列如序列表SEQ ID NO：1所示。
本发明还公开了一种水稻抗稻瘟病基因Pisj的共分离分子标记，所述共分离分子标记为SNP标记S6-43-32，所述SNP标记S6-43-32为显性标记，其PCR扩增序列如序列表SEQID NO：2所示。
本发明还公开了一种前面所述水稻抗稻瘟病基因Pisj的共分离分子标记S6-43-32的PCR扩增引物对，所述引物对的上游引物核苷酸序列如SEQ ID NO：3所示，下游引物核苷酸序列如SEQ ID：4所示，利用该引物对对待测水稻材料进行扩增，若能扩增出分子量为368bp的条带，则待测水稻含有水稻抗稻瘟病基因Pisj，若没有扩增出分子量为368bp的条带，则待测水稻不含水稻抗稻瘟病基因Pisj。
进一步的，为了提高S6-43-32共分离分子标记的准确性，在PCR扩增体系中加入OsACTIN1基因(LOC_Os03g50885)的引物对，扩增OsACTIN1基因作为PCR结果的阳性对照，OsACTIN1基因的PCR扩增引物对序列如SEQ ID NO：5和SEQ ID NO：6所示。
进一步的，前面所述的两组引物对对待测水稻基因组DNA进行PCR扩增，电泳检测扩增产物，若电泳显示只有一条条带，则待测水稻不含有水稻抗稻瘟病基因Pisj；若电泳显示为两条带，则待测水稻含有水稻抗稻瘟病基因Pisj。
本发明还公开了一种检测水稻抗稻瘟病基因Pisj的方法，对待测水稻进行前面所述的水稻抗稻瘟病基因Pisj共分离分子标记的检测，若有，则含有水稻抗稻瘟病基因Pisj，若没有，则不含有水稻抗稻瘟病基因Pisj。
进一步的，利用前面所述的两组引物对对待测水稻基因组DNA进行PCR扩增，电泳检测扩增产物，若电泳显示只有一条条带，序列长度为156bp，则为OsACTIN1基因的PCR扩增产物，待测水稻不含有水稻抗稻瘟病基因Pisj；若电泳显示为两条带，序列长度分别为368bp、156bp，则含有水稻抗稻瘟病基因Pisj和OsACTIN1基因。
进一步的，所述PCR扩增体系为15μL，包括：2×rTaq PCR MasterMix 7.5μL，上、下游引物各0.5μL，DNA模板1μL，ddH2O 4.5μL；所述PCR扩增的程序为：95℃预变性5min；95℃变性30s，61℃退火30s，72℃延伸30s，29个循环；72℃深度延伸7min；16℃保存；扩增产物在1.5％的琼脂糖凝胶上200V电泳分离。
本发明还公开了一种水稻辅助育种方法，通过前面任一种方法确定待测水稻稻瘟病抗性性状。
本发明还公开了前面所述水稻抗稻瘟病基因Pisj、共分离分子标记、前面所述引物对在水稻抗稻瘟病育种中的用途。
与现有技术相比，本发明的有益效果为：
1、育种过程可以快捷地对杂交后代是否含有Pisj进行鉴定，从而可以筛选出含有Pisj抗性基因的水稻品种或品系，加快水稻育种的进程。
2、将本发明提供的抗稻瘟病基因Pisj用于水稻抗病育种，可以提高底盘品种对稻瘟病的广谱抗性。
3、利用Pisj的共分离分子标记S6-43-32进行水稻分子辅助育种，只需要检测标记的扩增条带，就可以判断出抗性基因Pisj是否导入到底盘品种，不仅筛选快速、精确，不受环境影响，选择目标明确，而且节约了生产成本，提高了水稻品种或品系的选择效率和质量，直接实现了目标基因在水稻育种后代中的鉴定，为水稻稻瘟病抗性育种提供了新的抗病基因资源。
附图说明
图1“绥粳8号”接种不同稻瘟菌菌株后的叶瘟发病表型；
图2不同水稻品种中Pisj位点基因组结构示意图；
图3分子标记S6-43-32的检测图；
图4“空育131”和“绥粳8号”的接菌鉴定图；
图5“空育131”/“绥粳8号”的BC1F1的接菌鉴定表型，其中KY131代表“空育131”，S8代表“绥粳8号”，R代表抗病植株，S代表感病植株；
图6“空育131”/“绥粳8号”的BC1F1的基因型鉴定，其中R代表抗病植株，S代表感病植株；
图7“空育131”/“绥粳8号”的BC1F2的接菌鉴定表型，其中KY131代表空育131，S8代表绥粳8号，R代表抗病植株，S代表感病植株；
图8“空育131”/“绥粳8号”的BC1F2的基因型鉴定，其中R代表抗病植株，S代表感病植株；
图9“空育131”/“绥粳8号”的BC2F1的接菌鉴定表型，其中KY131代表“空育131”，S8代表“空育131”，R代表抗病植株，S代表感病植株；
图10“空育131”/“绥粳8号”的BC2F1的基因型鉴定，其中R代表抗病植株，S代表感病植株。
具体实施方式
以下将结合附图和实施例对本发明技术方案作进一步地详述。
“绥粳8号”是东北地区的一个水稻栽培品种。利用从东北地区搜集的31份稻瘟菌株对该品种进行接菌鉴定，发现“绥粳8号”对大多数稻瘟菌表现出较强的抗性，抗谱高达84％，见图1所示，这表明“绥粳8号”是一个具有广谱抗性的水稻品种。
为了解析控制“绥粳8号”抗性的遗传基础，以“绥粳8号”为父本，以感病品种“空育131”为母本，构建F2遗传群体。然后，通过喷雾接菌的方法对空育131/绥粳8号的F2分离群体进行抗性鉴定。同时在每条染色体上每隔10～15cM选取1个在空育131和绥粳8号之间存在多态性的分子标记，对F2群体的单株进行分子标记检测。然后通过ICIMapping软件，采用复合区间作图法对控制绥粳8号抗性的QTL进行扫描。在6号染色体上检测到1个抗性QTL，该QTL位于第6染色体的S6-40-3和S6-46-4两个分子标记之间。接下来，通过对该目标区域进行分子标记加密，最终将该抗性QTL定位在6号染色体S6-43-12和S6-43-29之间、大约90Kb的区段内，并与SNP标记S6-43-32共分离。将该QTL来源于“绥粳8号”的等位基因命名为Pisj。通过分析发现，该主效QTL为显性位点，即无论该位点为纯合的Pisj基因型或者杂合基因型，均能提高水稻对稻瘟菌的抗性。
为了对目标区域内的基因进行分析，将S6-40-3和S6-46-4这2个连锁的分子标记通过BLAST软件锚定到日本晴的BAC上(http://www.ncbi.nlm.nih.gov/genome/？term＝rice)，构建相应的物理图谱。结果显示，Pisj被锚定到6号染色体短臂上，且与Piz位点有重叠。
Piz是一个保守的基因座位，在该位点有许多NBS-LRR类基因成簇存在。在长期的进化过程中，不同的水稻品种的Piz位点含有数量不等的NBS-LRR基因串联重复。NBS-LRR的串联重复的数目和序列差异决定了它们的抗性功能。
鉴于Piz基因座的复杂性和多样性，通过一代测序的方法将“绥粳8号”的抗性基因Pisj所在的Piz位点进行测序分析，发现“绥粳8号”的Piz基因座有10个NBS-LRR基因串联重复，见图2所示。这与已经报道的该位点的其它抗性基因不同。而且在基因序列上，也与其它抗性基因存在差异。
本发明所利用的抗性基因Pisj被定位于“绥粳8号”6号染色体S6-43-29和S6-43-12之间约90Kb的片段内，并与S6-43-32分子标记共分离，S6-43-32分子标记为等位基因特异性共分离显性分子标记。利用Pisj基因供体品种“绥粳8号”与底盘品种杂交。利用S6-43-32分子标记对杂交后代进行选择。抗性基因Pisj及其共分离标记S6-43-32对水稻稻瘟病抗性分子育种提供了新的基因资源及新的利用手段，具有巨大的利用价值。
本发明利用Pisj基因供体品种“绥粳8号”与底盘品种杂交，使底盘品种获得该抗性基因。通过分子标记对水稻品种或品系进行PCR扩增，可以快捷地对杂交后代是否含有Pisj进行鉴定，从而可以筛选出含有Pisj抗性基因的水稻品种或品系，加快水稻育种的进程。
实施例1：抗稻瘟病基因Pisj的获得
1、本实施例中以“绥粳8号”为父本，“空育131”为母本构建的F2分离群体为研究对象。
2、采用喷雾接菌的方法对F2群体的679份单株进行接种，利用Tharreu D等的6级制统一标准进行表型鉴定。
3、采集群体679份单株的叶片，利用TPS法提取基因组DNA。
4、在每条染色体上每隔10～15cM选取1个在“空育131”和“绥粳8号”之间存在多态性的分子标记，对F2群体的单株进行分子标记检测。
5、通过ICIMapping软件，采用复合区间作图法对控制“绥粳8号”抗性的QTL进行扫描。
6、在6号染色体S6-40-3和S6-46-4两个分子标记之间检测到1个抗性QTL。通过对该目标区域进行分子标记加密，最终将该抗性QTL定位在6号染色体S6-43-12和S6-43-29之间、大约90Kb的区段内，并与SNP标记S6-43-32共分离，将该QTL基因命名为Pisj。
实施例2：Pisj共分离分子标记S6-43-32的PCR扩增引物的开发及水稻材料的基因型、表型鉴定方法
1、水稻稻瘟病抗性基因Pisj共分离分子标记S6-43-32的获取方法，包括以下步骤：
(1)、利用CTAB法提取“绥粳8号”的基因组DNA，利用二代测序技术对“绥粳8号”进行基因组重测序，获得“绥粳8号”SNP位点，
(2)、以参考基因组日本晴的序列作为参考序列，在与“绥粳8号”间存在差异的SNP位点处设计引物，正向引物的3’端是差异的SNP，并在引物序列的倒数第3位碱基处引入一个错配碱基增强其特异性，错配碱基的引入以A、C、G最好。然后在SNP位点的下游设计反向引物。利用上述设计原理，设计分子标记S6-43-32的扩增引物对。
(3)、由于S6-43-32分子标记为等位特异性SNP显性分子标记，为了提高S6-43-32分子标记在PCR扩增体系中的准确性，我们在OsACTIN1基因上设计一对引物，应用步骤(2)中所述分子标记的正反向引物在扩增体系中再加入OsACTIN1基因上的一对引物对“绥粳8号”的基因组DNA进行扩增，PCR扩增后进行电泳分离，在“绥粳8号”中能扩增出两条条带，分子量大小分别为368bp和156bp的目标带，即获得的分子标记S6-43-32即为水稻“绥粳8号”抗稻瘟病基因Pisj的共分离分子标记。
2、基因型及表型鉴定方法
PCR程序参见萨姆布鲁克等，2002，分子克隆实验指南，第三版，黄培堂等(译)，科学出版社介绍的方法，并按照中国科学院遗传与发育生物学研究所农业资源研究中心的条件和要求，做了修改。
水稻抗稻瘟病基因Pisj的核苷酸序列如序列表SEQ ID NO：1所示。
利用S6-43-32标记的PCR引物扩增到的PCR产物的DNA序列如SEQ ID NO：2所示。
S6-43-32-F：(SEQ ID NO:3)：AGCAAAAAGTTGGTTTCAGTG；
S6-43-32-R：(SEQ ID NO:4)：AGGTAGATAGCAGGTGGTG。
OsACTIN1-F：(SEQ ID NO:5)：TGGCATCTCTCAGCACATTCC；
OsACTIN1-R：(SEQ ID NO:6)：TGCACAATGGATGGGTCAGA。
利用OsACTIN1-F和OsACTIN1-R引物扩增到的PCR产物的DNA序列如SEQ ID NO：7所示。
基因型鉴定的具体步骤：
(1)、提取水稻植株的基因组DNA；
(2)PCR扩增，以步骤(1)的水稻植株基因组DNA为模板，将上述DNA加入到PCR反应体系中，对所述的水稻植株基因组DNA进行扩增得到扩增产物；
所述PCR体系为15ul的体系：水稻植株的基因组DNA20-40ng，2×rTaq PCRMasterMix 7.5μl，上、下游引物浓度10μM/μl各0.5μl，ddH2O 4.5μl。所述2×rTaq PCRMasterMix由TAKARA生物工程有限公司生产；
所述PCR的反应程序为：首先在95℃预变性4min，之后进入PCR循环扩增，循环扩增包括95℃变性30s，61℃退火30s，72℃延伸30s，共三步，循环运行29次，最后再72℃延伸7min后，16℃保温，得到扩增产物；
(3)1.5％的琼脂糖凝胶电泳检测，称取1.2g琼脂糖粉，加入80ml的1◇TAE缓冲液，微波炉加热溶解后，不烫手时加入100μl浓度为0.2mg/ml的EB溶液，30min后点样，在200V直流电压电泳15～30min；读取各样品的PCR带型。
根据PCR带型从而判断出是否含有“绥粳8号”的Piz抗性位点。当PCR产物扩增出两条条带，分子量大小分别为368bp和156bp，则含有“绥粳8号”的Pisj基因。且稻瘟菌接种鉴定表现为抗病，当只有一条大小为156bpOsACTIN1基因的条带时，则不含有“绥粳8号”的Pisj基因。且稻瘟菌接种鉴定表现为感病，见图3。
表型鉴定的具体步骤：
(1)将水稻催芽露白后播种到育苗盘上，进行水培培养；
(2)每隔2天换一次营养液；
(3)待水稻长至3叶1心期时，利用喷雾法对水稻进行接菌鉴定；
(4)水稻接种5天后对表型进行调查(表型结果见图4)。
实施例3：稻瘟病抗病品种的选育
发明人挑选粳稻品种“空育131”作为改良品种，该品种对稻瘟菌的抗病性较差，含有Piz感病等位基因。同时选取含有Pisj抗性基因“绥粳8号”作为供体品种。利用分子育种的方式提高“空育131”对稻瘟菌的抗病能力。
利用本发明的Pisj抗性基因的分子标记S6-43-32，分别对BC1F1、BC1F2、BC2F1代植株进行鉴定，分别得到两种基因型的材料，记为R(含有Pisj)和S(不含有Pisj)，见图6、图8、图10。然后对这两种材料的抗病表型进行稻瘟菌接种鉴定，见图5、图7、图9。结果表明基因型为R型的材料均表现为抗病表型，基因型S型材料均表现为感病表型。
上述结果说明，本发明的水稻抗稻瘟病基因Pisj及其共分离分子标记S6-43-32，可以用于通过分子育种手段培育稻瘟病抗病新品种。
以上内容是结合具体的优选实施方式对本发明所作的进一步详细说明，不能认定本发明的具体实施只局限于这些说明。对于本发明所属技术领域的普通技术人员来说，在不脱离本发明构思的前提下，还可以做出若干简单推演或替换，都应当视为属于本发明的保护范围。
序列表
<110> 中国科学院遗传与发育生物学研究所农业资源研究中心
<120> 一种水稻抗稻瘟病基因Pisj及其应用
<130> 2018
<160> 7
<170> SIPOSequenceListing 1.0
<210> 1
<211> 88769
<212> DNA
<213> Oryza sativa
<400> 1
ttgacattgt atcacagggc accacccatt ccctctcctt aatttttgtt tgactatagt 60
aaccatatca atcaaatagg tgtgaaagct aacctagatt atttctgatc acactgatca 120
cattctcact agctattcta cactttctac cttcacatag gacgacaagt gtaccaacct 180
actgataaat tttcaagcct catttttctt ataagttatg gccaaaatta aaattttaaa 240
aattagtttt aaattatttt gatgttgttt catcaccttt ttctagcttt agctaaaatg 300
atataatgat agagaaaaat aaaagtcata acctaaaaat aatttttagc tcgtttttta 360
tagcttatta gccgcagact attcaactcg accctccgtg ttttcccata taagcccata 420
ccatggctac gtttgaaact acaactctac aagttctcat attttatttt cttatttttc 480
acaatttttc acaagtacgt ttttgaaact gtaaaacgac gtgtgtttct aaaattttct 540
atagaaaaat tactttaaaa catcatataa atttatttta atttttttta actaatacta 600
cctctgtcct agaaagactg cagttttgca ctattcatat ccaacgtttg accgtccgtt 660
ttatttgaaa gttttttata attagtattt ttattgatat taaatgataa attatgaata 720
atactttatg tgtgactatt ttttattttt ttaataaatt tttaaacaag acggacgtgg 780
ttagtatttt tattgttatt agatgataaa ttatgaataa tactttatgt gtgactattt 840
ttttaatttt ttttataaat tttcaaataa gacagacaga caaacgttgg gcaccaaaat 900
tcataactac agtcaaaatg ggacggaggt agtaattaat taaccttttt tagaagaaat 960
gagaagttcg aacacaaccc gcacacccaa accaaggggg tatttagatt gaggggtgta 1020
aagttttggc gtgtcacatc ggatattata tatgatgttg catagggtgt tcggacacta 1080
ataaaaaaaa tcaacttatt aagcctaatt aatccgtcat tagcacatgt ttactgtagc 1140
accacattat caaatcatgt agcaattagg tttaaaagat tcatctcgca aattagtaat 1200
aatctataca attaactatt ttttagccta tatttaatat attatgcaga tgtccatacg 1260
ttcgacacga cgcgatctaa aattttcggg tgggctcatc gaaagcaaac aaaacgaacc 1320
cccgcacacg aacggtcaca cgctgatctt ttctccaaac ccaaaaaaga aaagaaaatc 1380
agaaaaagtc ccgccactac ctctactact gccggcccta cccccacaga ttccaggcgc 1440
cagctaagtt ccccggaaat tcgtgcttcg ccggattggg gcttcgccgc caattccatg 1500
ttcggtgagg ggtggcgggt gaatgtgttt ctttttttca actgatttta gagttggtga 1560
ttggttgcgc cgttctagat cgagctgacc cgcctgcgtg ttggattgta ttgattagag 1620
gaagggaggc tgattagcac gaggtgtggg gaaaaattag ttgtaaaaaa aattggaggg 1680
gttaatagat gggcgtttgt taagtgacgt aaggcgaaag tgatgttatg ctgttctggg 1740
ttagtaaggt tcttggcaat cagttttggt caaatcttac tggttcatag gtgttttggt 1800
cgaatttcag cttcagatga agtttgtccc tcgtttccag gattgttggc ggtcagttct 1860
tcatcgttag gcatttaatt ggttgaacag gaaattgggg tcatggtaga atgcgaagtt 1920
tctgaaaaca taagtagaga acaaaagtag gagaattttg tcaattaggt acgatggaag 1980
gtcaccgtca gcttgttcaa ctactgttag gttcaaactt tgatcgtgct tgagatttct 2040
ttgctaaaat aaggggatat gtggcaaagg gagaataaac catgatatga tttttgttac 2100
tgactatagt ggctgtaaat gagaacttgt ggaatcacag aaagttccaa aaaacaatgc 2160
agaaattgta tccttttttg tgctttgcct agtccatttt ctgacttctg agatgaggtc 2220
cccgcatcac atagaactgc aattgcaatt caatgcattc atgcaacaaa cagacagact 2280
actgtcctat aacatgagtt atgacttacg agtagccttg catattgtac atgcataagc 2340
caaagctgct tgtttattgt aaaccagata ctctgtacca taaaaaccat aatccattaa 2400
gttttcttgt ttactttgtt gaacattata ctagatacct atggtgttga ctgttacata 2460
acattctgtt caccatttgg caattttccc cccttagtga cattcattct tttgagattt 2520
cagtcatgcc ttccacatga caaaatgttg cattcaacta tgatgtttgg aattgggaaa 2580
cggtactcat acttaatgtt tcttttacta attctgattg aaagcatgca acacaccttt 2640
taatccacat gcacatacat caatcaatgc tttggagaga gtgcatcatg gatcaatcat 2700
attgatggtt tattagtaaa cattttacag caattatttg tgcacatggc cctcatacaa 2760
tttttgttgg gcattcaacc cttgttttga ggtgttaaat aaattatttt ttattatgtt 2820
gtcaaattgc ctgacctttg tattggtggt tgggcatcta gctgtgcact gtcaataact 2880
cctggcggtg ctcctctttt tcctggctgt tgaacttcca atctgaccat tcattatgtt 2940
catatcttgt acacatgtgg tgagatgtca attgctgtcc attgtatgtt attatatttt 3000
acttagcatt gttcaactgt aatagacagt gatatatcat acatatcaaa tgaaaccaac 3060
acagacaaag catatgctgt gttgtcatct tcttatttct tgtactgttc tgttctgtct 3120
tgaaggttag ctgtgttagt catggcccac tatggattct caaaaggcat cctttatgtt 3180
cattagtttc atctttttct attttccaat tttattagat ggctatacac gactttgttc 3240
acacataatc tctaattatc aatcgtctgg tcatgcttgg caggcctcaa tcaaatgcca 3300
gcccacccat gggaaatggg ccaggattga ggcctgccga aaccaggtgt agaggtagat 3360
acacccttta atgtggatca tggttccttt ctaggaggaa gcaggtcgac ccttgcccaa 3420
gtttggtgaa tgggatgtca acgacccagc ttctgctgat ggattcacag tgatattcaa 3480
caaagccaga gatgagaaaa agggtgggaa tgggcaagat actgattcac cctgcaaaga 3540
gactaggact gagagggtgg aatcatatgc ccccaaaaca aactcggtat gtctattaca 3600
ttactcacgc tgattataat atcagaattt caatttctcc ctgattaata taaagttatt 3660
ccttttctct tgaactagat aactagctag atgtcattaa tctcattgtt ttcatgtgca 3720
gaagaaatgg ttttgctgtg tgacatccag tcctacacaa tcttgatgaa aacgagttcc 3780
atgggttgca aaattactat cctttaattt tgctatatac atactatcca taagaccttg 3840
tagagatgcc cagactctgc tgtggtgctt gattgggcat ctcttaaaac tctgaggtgt 3900
gtgtatgtat gtgtgagggt tatcagatgc acattcggat aaatgaactt ctgattgtaa 3960
ttctagcctt ccgtcctgtg acattctgtg atgtagtcac attctgtgat gtagtcgtta 4020
ttcagtgata taatgaatct cacctattga tataactgca cactgtttct cttgctttcc 4080
gaggaaacaa acactgtttt accaaagttg gctgtgctcc aaatgaggat taaattccgt 4140
atgattctca aattgcaatt gcaattttga ttttctcacc cattaatatg tgaatttgga 4200
attttctttc acgtgtcaaa ttcgcatatt gcaatttttc taaaggatac aagcacagta 4260
tagctcaatc tcttgcaggc tgagttggta gcaatgcaat tggcgatggg agttttctta 4320
atattgtagg catcacattt ctaacacaca atgcaacggt gtatcgacta ggtgataagg 4380
tagcaaagga tgtgagatcg caacagctca atccttcagt ttatgattgt caaaatatct 4440
tgagtcttgc tcaccctaga aaccttcgtt atgcttgaga tttgaaaaag agcaacgaat 4500
tgtattacaa gtcatgtgct ctgtttctga aatggaaata atactgacga gttctaaaga 4560
aaaaaagaac catgcattca tgcctgtaca ccgccgcagc cgacgaggcc accctcctgc 4620
tcggcgtcca gaaggagatc tggtaagtgc gtgcacgctt ctgtaactcc ataccacatc 4680
ggtgactctc catggcccct ccgcggcggc gtgtcggcga cctcggcttc catggccgcc 4740
gccgccgccg cctcacagcc ctgcttcgcc gcaatttctt ttttggtctt ttggtgattc 4800
caacggattc aggatcaaaa atgaaacaat ccggagagcc tattcacgct tcgcatttca 4860
ctaccccaaa cttgtggatt gtgaccatgc aaaccaaaat tccttcagtt tcatactttc 4920
atatatgctc aaatttccgt tgttttcgta gaaacaaggt tatttatccc acttacatct 4980
tgcaaaaacc aaagtacagt tactcttatc cgattttcat ctatacacat gctagacgaa 5040
tgcattgtgg tccaattcaa atttctgata tattagctac taaccaagat gctcccatgc 5100
catgagcgca agttccattg acaaggaaaa acactttttt atgatttgag caagttccac 5160
gctggcgtcc tcgttggcac accacgtaag ctaaagtggc aaaaaaaaga aaagaatttt 5220
tctctctaaa gtgagtcagt gacaaaacct taagtgcaaa attaaaatgg gtatttggat 5280
aatcgccaaa tctaaaagtg tcaaatagtt aaattcccct tctatatttc tctcttcagt 5340
ggcaaaaact taagtgcaaa aataaaatgg gtatttgcat aatcgccaaa tctaatagtg 5400
gcaaatagtt aaattcccct tttatattag tccttgagga gctacggcaa tgtttgctag 5460
caccggacaa gatcatccaa taaaaactta gagattatac ataacagtgt cactgttact 5520
agcaaaattt tccttcctat tgcatggtta gtacattttt ttttgtgaga aatatatggt 5580
caatgccatt tgcaatatac ctccaggctc tagtcaatat aagattacat acatatgcga 5640
tctacatttt gtactaggtc tctaccaaaa gaaggggttt gtacccttct gttctcttaa 5700
attaatgaga cgatttgtag gatctttctt tttcaaggga ttgtgggagt tctgactctc 5760
atggctaacg aggcgctctg aatactagta gtttatttcc atccatctat ttatttattt 5820
taataaattt tattgctttt gaatttatat ttgtaggtac atcaaggacg agctgaaaac 5880
tattcaggca ttcttaagag ctgctgaagt aacaaagaag aaagatgact tgctaaaggt 5940
atgggcagag caagtacgag atctgtcata taacattgaa gattgcctag acgaattcaa 6000
ggttcatgtt gagagccaaa gcttggcaaa gcaactaatg aagcttggtg aacgccatcg 6060
aattgctgta cagattctca acttaaaatc aagaattgaa gaagtgagca acaggaatac 6120
acgctacagc ttaatcaagc tcatttcctc tataaccaca gaggatgaga gggattccta 6180
cctagaagat gctcgcaatc gatcaggtag caacactgac gagtcagaac ttgtgggctt 6240
tgccaagact aaagatgagt tgcttaaact gatagatgtc aatactaatg acggtccagc 6300
taaagtgata tgtgtggttg gaatgggtgg attaggcaag actacccttg caaggaaggc 6360
atatgaaaac aaggaacaca tgaagaactt ctcgtgttgt gcttggatca ctgtgtctca 6420
gtcatttgac aggaaagaaa ttctgaaaca aatgatcagg caacttctgg gtgctgattc 6480
attagacaaa ctcttgaaag aatttagtga gaagttgctc gtgcaagtcc agcatctcgc 6540
tgatcacttg gttgaagggc taaaggagaa aaggtacttt gttgtccttg atgacctatg 6600
gaccatagat gcatggaatt ggattcatga tactgctttt ccgaagatta acaacagagg 6660
tagtcgcata ataataacaa cgcgagatgc tggcttagct ggaaggtgta cctctgaatc 6720
acttatttac caccttgaac cgttacatat agatgatgct atacacttgc tactagcaaa 6780
gacaaacata agacttgaag acatggaaaa tgatgaggac ttgggcagca tagttacaaa 6840
attggtaaaa aggtgtggtt atttaccgct ggctatactc acaataggag gcattcttgc 6900
tactaagaag ataatggaat ggggaaaatt ttacagagaa cttccttcag agcttgagag 6960
caatccaagc ctagaagcca tgaggaggat ggtgacccta agctacaatc acttaccatc 7020
tcatcttaaa ccatgctttc tttacctaag tattttccct gaagattttg aaattcaaag 7080
agggcgcctg gtagatagat ggatagcaga gggttttgtc agagccacag atggggtgaa 7140
cattgaggat gttggaaata gtcactttaa tgagcttatc aacagaagtc tgattcagcc 7200
ctcaaaagtt agtacagatg gagttcatgc gtgatatcat agtttcaatt tctagagagg 7260
aaaattttgt gctgttgact agggagaaga tcactgttgt agcggaggag agcatccgcc 7320
atctagcatt tcatgggagc aaatgctcaa agatatgctt ggagtggaac catctgcgct 7380
cagtaacttt gtttggtgac agacctgcgg ggcgaacacc tgcactttgt tcaccacaat 7440
ttaggatgct gagagtgttg gatctggaag atgcaaaatt caaattcaca caaaatgata 7500
tcaggaatat agggttgttg cgccacatga aatatttgaa ttttgcaaga gcctcaacta 7560
tttatacact tccaaggtcc ataggaaaat tgcagtgctt gcaaattttg aacatgaggg 7620
aggcaaatat ctcagcacta acaactgagg tgactaaact ccagaatctc cgtagcctcc 7680
gatgcagcag gaggtcaggt tctggttact ttagcataat agataatccc aaggaatgct 7740
tgatgatcac catgtgctta ccgatggttt tctcaacttc aataaatttc agtgaccgtg 7800
tgaagttaat tcctgagata tgcatgtcat gttctacccg ttggtctgat acaaagggtg 7860
tgagggtgcc aagaggaatt gacaacctaa aagagttaca gattctagaa gtcgtggaca 7920
tcaacagaac tagtaggaag gcgattgaag agctggggga gctaattcag ttaagaaaat 7980
taagcgtgac aacaaaaggc gccacaaata agaagtatca gatattttgt gcagcgattg 8040
agaagctctc ttctctgcaa tctctccgtg tggatgctga gggattctca gatactggaa 8100
cacttgagtg gctcaattcg attgcttgtc ctcctccatt cttgaagaca ctcaagttga 8160
atggatctct tgcagataca ccaaactggt ttgggaacct taagcagctg gtgaagatgt 8220
gcttatccag atgtgtgcta caagatggta aaactatgga gatacttggg gcactgccca 8280
accttatggt tcttcgtctt tatcgcaacg catatgctga cgagaaaatg gcattcagaa 8340
ggggaacatt cccaaatctc aggtgtcttg atatttactt gctgaagcaa cttagagaga 8400
taagatttga ggagggcacc tcgccaacaa tggaaagtat agaaatttat ggttgcaggt 8460
tggaatcagg gattattggt atcaagcacc ttccaagact taagattatt tcgcttgaat 8520
atgatggtaa agtcgcgaag cttgatgtgc tgcaagagga agtgaataca caccccaatc 8580
atactgaact gcaaatggca gaggatcgaa gtcatcatga cctaggaggt atattatata 8640
aggatcaaat atagcatatg aacaatgaaa atcgcatatt gagcttgcca gtagttggct 8700
atactattga acttgctatt atagtgggta gtggagccat gcaaggacct ggtatctaaa 8760
gcgcacctaa accgtaatgt ggacagccat tatagttaaa gttaggggga atatgattct 8820
cttcatgtgc acctaaaccg taatgtgcag tgaaacgaac gctatgatat gatgataagc 8880
ttaattcctc tctctgctca gactgttcag ggcaaaaact accaacgaga gcttgtctcc 8940
ttgtgcggtc gtgagcttgc ttgtgctaag cttgaaggga gagtcgaacg aatccatggc 9000
ggagacggtg ctgagcatgg cgaggtcgct ggtgggcagt gccatcagca aggccgcctc 9060
tgccgctgcc aatgagacga gcctcctgct cggcgtcgag aaggacatct ggtacgtact 9120
gcactgctct cgtttatcct agcaagttct taggctctta atctcgaaat tgaggaacac 9180
catgaaacac taaaagagag ctcgaagact aggaaagaaa actagaagac taagctttga 9240
aagtcttcta aatccaagca tctcgacatt gatcatcctt gtgcaacatc atcccttcct 9300
attgcttcac cagaatcggt gtcccttgtg gagatctctg tcgtagcgtc aaggggagaa 9360
tccgagaagc agaactagtc cgcgctgcct tcgctacgcc atctccgcca tagaggatct 9420
catccacgaa acatccacca tccaaacggg aaactgtttt aaacactcgg ttggatgttc 9480
acccgtttct tgcctgtcat ctaaatggtt atgaaaaatt ttcaaaaaaa aacatgatag 9540
gttaatatat aatatatcat ctcacaaata tgcaagttca aattcaactt ttataagttg 9600
taaaggcaag agctagctca cccgtcccac acacacaccc aacgacgtca caagcgcctc 9660
cgaacaacgc caactgataa cttggcagct cctacgtgcc gacgtcgcgg tacttgccgg 9720
cgctcctagc gcacgcaccg tcgaaccaca ccgtcaccga ccaactaccc accgccgccg 9780
acttctgcct catctgccat cgtcgcccta gcccaagtta tcatcgtggc aattgccgag 9840
gctcctaagt gtgccacggc cgaggcaaag ttctaattaa ctgaatcaga gtatcagaca 9900
gccaccaccg acgctacttc tgcttcatct gccatcgccg tactagttca agttgtcgct 9960
gtggcaatca tgggccctcc tagcgtgcca cccaaccgga cagccacgac atcccccatc 10020
actgttgtta ttgccgcgcc ctgaccccta tcgtcgtcgc tcttagcgcg tcgtcgagcc 10080
gaccagccac tgtcgtgcag atgaaaaaaa aaaacacata ttggcctgag agatctgctt 10140
agttccagtg caggtccaac atgctgtgag atgcgggcgt gccagtcagt ttgatcttgc 10200
aactgacaag atatataaat agcagataaa acagcctatc gactaacaag ccgatggagt 10260
aattccagcc gatagccgat attagccgat gccgattcta gccgatgtcg atagggtttt 10320
gaactatcgg ctatatgtcc aatgtaggca atgatataaa gacaattggc tgatgataat 10380
aaaatataaa aatataatcc aatagaaacc aatcggctaa taataagtat tgatccgata 10440
gttaaagcat acatcggcta aaagtccgat gtcataaaat ccaatcgatt tagataaaca 10500
gtgaaacctt tgttgcaatc ggctaaatcc aacttgtatg taatcttcgt aagccgatga 10560
acgtccagat aacttatcgg ctagcacctc gataaaacac tagcatgaac ctatcggctt 10620
aacaagattt atattatcaa caacaatcta gtaggtcgga cctaaccgat gcaacacgta 10680
ttagatatga taatctaata ctcgatgagc caatagatct gtctaatgtg atggatataa 10740
caaatctatt tataaaagca ttgcgattgt agagatatat cggctaagac agaatatcag 10800
acctaactaa accgatgcgt ctctaaacac aatgcaatta attagagata taattgagat 10860
atcagctagg caaatatatc aaccaaactg gagcgatcca agagatcgga gcaatgcagc 10920
cttgaacaac accaatgtag ccgatggatt caccagggcc gacggaacgt aggacttacc 10980
ccttccctga agatcgggct gaaccaatgc agtcccacgt caggtgccaa attccgccgg 11040
ttgacaagta aaacctcaga aaagaggatg acgatgcgcc gagagtagta ttgatcgaga 11100
gataaattgc aatgaccctg gatgtacata tttgtaccca tgggtagata ttagttcttg 11160
taggacaaga aagaaacttt cctaaagata aaatgaaaac ataaagtctt tattggatac 11220
taaacacact ttcctaaaga taaaaggaaa ctaaaccctg cctaattaat agataaactg 11280
ccatgtcgta tcctccttga actcggactc ttttagataa gcttccttta actaatcttt 11340
acccgaatcc atcaagaata caaatgttgg cattgatagt tttcatcggt caattctagg 11400
actttgaagc cgatactgac tctaagccga tgactacttt gggcttacca aattttgttg 11460
ttaacatgtc gcgaccacca tcaccggcca gccaccctga tcattgttgt tgactcagca 11520
ttcgccaggc tgagcagtcc acatacatgc cgccatctcc atggcagtgt cgttgccgcc 11580
cctttctcct agagccgccg cagcgctctt cgacacacct actgcatcgt cgagcagtcg 11640
tgctaccacc tcctccatcg accatagccg cctcttctgc tgcaccggat ccacccacac 11700
caaccaccag atacagtcaa gccctcattc ccggatccca tatccatcca tgccactact 11760
gtgctgccca gtccaaggaa tggagcgaag gaggaagccc cgccgctgcc ctcccggcgg 11820
ccacatgcac tccagtgcct tgctccgacg gcagcgaggt tggaaaatgg gtggcagcgg 11880
ctagggttta tctggggaga aggaaaagga gagggagggg gggggggggg gggttccact 11940
tccagcttaa ttagcctaga tcttattgac aaatcagttg ctgggtgcac aaacatgtta 12000
ttttttttgc atgaccaatc ttgaacactt aggtatgtta gttgagtgga cactggtcta 12060
tctgaaacat ctcttcacat ggaggctgcg aatgagtttt ctttttgaga gaccaaagtt 12120
tcgttgtatg ttaagtgata aagccttggt aagaaatgct accacaaacg aactaataac 12180
tccaaacgta aagtggagga acccgtatgg gtgactcgag tggcgacaaa ctctagcacc 12240
tccacctcca tggacgggct gcggcggtgc tttcggcatc ccagtcttct tggaggcatc 12300
atctagaatt aaggtcttgt tattgcttag catgccttag ggcacgtcca gtgtttagtt 12360
cgactaaaac ttccatgaaa gccaaacaaa agttctgttt gaccaccaca gtgtaaaaat 12420
cgattgtggg acccatgcaa aaaaatcaca atctcagctg cctatgctct cctcctggac 12480
ctgatagccg tgcacaacaa atattttttt aaactggatg tgttcggctt ctctttaaag 12540
atcgtttttt cctctgacac ttaccaaccg gctttcacag tgtggtcagt tctttttttt 12600
tttacgcaaa gtttgatttt agtcagacac gggaggatct gttaagcagg cttggaaatt 12660
tcggacccct ccaatacaat attattttag ccaaaatttt ctaatttttt aatttttcat 12720
gaattttggt aatatttgtt ctaatttaac taaattttgt tcaaaatttc ggtctatcag 12780
tgacctccga tcaaatcagt taaaccgaga aaataaacca tgctcttaag agagtttggt 12840
atggttcaat atcaaaactt atagtcttgc aattttttct accctttatc tttttccctg 12900
actatttagt atggatcgtt taaaaaaaag aaagcccatt ggtgaccaag ggcttgtttg 12960
attcaagacc atccctagcc ttaccaacct tttggcaatg gcaaaaattg gttgttgcca 13020
aaaatattgg cacaaattgg ctaagcctat gattggtttc taccaaagtt gaattttggc 13080
attcaatcaa gccaaataat ttggcaataa cattttctta tctatggata taacatatgg 13140
caaatatttt ggcattacca ttttcttttt gccaaacatg ttattccttt tgaatgacca 13200
atcttgacac cttatgtatg ttagtagtgg aatcgacact attctatcta aaacatctct 13260
ttacatagag gccgctaata atttttcttt gagataacca aattttcctt acaagttaag 13320
caacaaagcc cattggtaag atatgctacg acaaatgaac taataactcc aaacgtaaag 13380
cggaggatcc cgcatttccc acgtgggtga ctcgagcggt gacaaaccct agtacctcca 13440
cccccttggg tgggttgtgg tggcactttc ggcaccgtat tttccttgga cggatcattt 13500
agaaagtcct attattgcct agtatgcctt gacagtttag gcaacactct tggatggtgg 13560
tgtcctttgc cctggtgatt tagtagccca tggatgttta gttatttgga catggtgttg 13620
gatggtgcgc tcgtgggcct gttgtaggtc tggtgccaac cagtcatgct tagaaatagc 13680
cggataggtg cacagtgcta gttctttact tggtggtttg tgcagcgcta tcgacatgtg 13740
gtggtgtgct ttttctttgt ccggataata atctcatagg gctatactct tgttattttg 13800
ctgctatatt attatgataa cttggtatgg ttcgtttttt ctttttttgg aaaaacacct 13860
agttgatcaa gggcttgttt ggttcaagtg cattcctaat cttacctttt cttttttttt 13920
tcaatggcaa gaattgttca ttgcaaaaaa aaaaagagat aaaaattggc taggcttacg 13980
ttttggttct taccaaagtt gtactttgag accaaatata tggcaaaatt ttggcataac 14040
cttttttttt tgcttggttg agcttggtac aaaccaatca gtcacaaaat agactgtcat 14100
gaatcacgcc tactaaattc ctttgaaccg aactagaata tatttgctct taaaagattt 14160
cttgatttca attggtacca tttactagta gaaacttaaa tttaaatttt aaaaacaaaa 14220
tcataatatt gttgttatgg aaattttagt cattttagta cttttgtaat atatgagttg 14280
ggttatactt gagatatcct aaattgcttt aagatgaaca attgctaggt atatcaaaga 14340
tgagctaaaa acaatgcagg cattccttag agctgctgaa gttatgaaaa agaaagatga 14400
actattaaag gtttgggcag agcaaatacg tgacctgtcg tatgacattg aagattccct 14460
tgatgaattt aaagtccata ttgaaagcca aaccctattt cgtcagttgg tgaaacttag 14520
agagcgccac cggatcgcta tccgtatcca caacctcaaa tcaagagttg aagaagtgag 14580
tagcaggaac acacgctaca atttagtcga gcctatttcc tccggcacag aggatgacat 14640
ggattcctat gcagaagaca ttcgcaatca ctcagctcga aatgtggatg aagctgagct 14700
tgttgggttt tctgactcca agaaaaggct gcttgaaatg atcgatacca atgctaatga 14760
tggtccggcc aaggtaatct gtgttgttgg gatgggtggt ttaggcaaga cagctctttc 14820
gaggaagatc tttgaaagcg aagaagacat taggaagaac ttcccttgca ttgcttggat 14880
tacagtgtca caatcatttc acaggattga gctacttaaa gatatgatac gccaacttct 14940
tggccccagt tctctggatc aactcttgca agaattgcaa gggaaggtgg tggtgcaagt 15000
acatcatctt tctgagtacc tgatagaaga gctcaaggag aagaggtact ttgttattct 15060
agatgatcta tggattttac atgattggaa ttggataaat gaaattgcat ttcctaagaa 15120
caataagaag ggcagtcgaa tagtaataac cactcggaat gttgatctag cggagaagtg 15180
tgccacagcc tcactggtgt accaccttga tttcttgcag atgaacgatg ccataacatt 15240
gctactgaga aaaacaaata aaaatcatga agacatggaa tcaaataaaa atatgcaaaa 15300
gatggttgaa cgaattgtaa ataaatgtgg tcgtctacca ttagcaatac ttacaatagg 15360
agctgtgctt gcaactaaac atgtgtcaga atgggagaaa ttctatgaac aacttccttc 15420
agaactagaa ataaacccaa gcctggaagc tttgaggaga atggtgaccc taggttacaa 15480
ccacctacca tcccatctga aaccatgctt tttgtatcta agtatctttc ctgaggattt 15540
tgaaatcaaa aggaatcgtc tagtaggtag atggatagca gaagggtttg ttagaccaaa 15600
ggttgggatg acgactaagg atgtcggaga aagttacttt aatgagctaa tcaaccgaag 15660
tatgattcaa cgatcaagag tgggcatagc aggaaaaatt aagacttgtc gaatccatga 15720
tatcatccgt gatatcacag tttcaatctc gagacaggaa aattttgtat tattaccaat 15780
gggagatggc tctgatttag ttcaggaaaa cactcgccac atagcattcc atgggagtat 15840
gtcctgcaaa acaggattgg attggagcat tattcgatca ttagctattt ttggtgacag 15900
acccaagagt ctagcacatg cagtttgtcc agatcaattg aggatgttac gggtcttgga 15960
tcttgaagat gtgacattct taatcactca aaaagatttc gaccgtattg cattgttgtg 16020
ccacttgaaa tacttgagta ttggatattc gtcatccata tattcacttc ccagatccat 16080
tggtaaacta cagggcctac aaactttgaa catgctgaga acatacattg cagcactacc 16140
aagtgagatc agtaaactcc aatgtctgca tactcttcgt tgtataggac agtttcctta 16200
tgacaacttt agtctaaacc acccaatgaa gtgcataact aacacaatat gcctgcctaa 16260
agtattcaca cctttagtta gtcgcgatga tcgtgcaaaa caaattgctg aattgcacat 16320
ggccaccaaa agttgctggt ctgaatcatt cggtgtgaaa gtacccaaag gaataggtaa 16380
gttgcgagac ttgcaggttc tagagtatgt agatatcagg cggaccagta gtagagcaat 16440
caaagagctg gggcagttaa gcaagttgag gaaattaggt gtgataacaa aaggctcgac 16500
aaaggaaaaa tgtaagatac tttatgcagc cattgagaag ctctcttccc tccaatctct 16560
ctatgtgaat gctgcgttat tatcagatat tgaaacactt gagtgcctag attctatttc 16620
atctcctcct cccctactga ggacactcgg gttgaatgga agtcttgaag agatgcctaa 16680
ctggattgag cagctcactc acctgaagaa gttctactta ttaggcagca aactaaagga 16740
aggtaaaacc atgctgatac ttggggcatt gcccaacctc atggtccttt atctttatgg 16800
gaatgcttac cttggggaga agctagtatt caaaacggga gcattcccaa atcttagaac 16860
acttcgtatt tacgaattgg ctcagctaag agagatgaga tttgaggatg gcagctcacc 16920
cctgttggaa aagatagaaa tctcttgctg caggttggaa tcagggatta ttggtatcat 16980
tcaccttcca aggctcaagg agatttcact tgaatacaaa agtaaagtgg ctaggcttgg 17040
tcagctggag ggagaagtga acacacaccc aaatcgcccc gtgctgcgaa tggacagtga 17100
ccgaagggat cacgacctgg gggctgaagc cgaaggatct tctatagaag tgcaaacagc 17160
agatcctgtt cctgatgccg aaggatcagt cactgtagca gtggaagcaa cggatcccct 17220
tcccgagcag gagggagaga gctcgcagtc gcaggtgatc acgttgacga cgaacgatag 17280
gtcagtcact ccctacatgg cagcttaatt aacttgtttc taattctctt cttgttcagt 17340
attagccatc aggtgagggc gatgatttca actcactttt catctctctc gttttcttaa 17400
cctgacagcg aagagatagg cacagctcaa gctggctgac gatctcctcc cccatcagcg 17460
tcgtcatcag cgaacagata gggcagggct tccctgcttc tgcgtgcacc tcaccgctct 17520
gactcggagg gacatgatga tcaatgaggc ttccagtttc caaatgtgtg gctaacacac 17580
caggttgtcc ctatccgagg tatgaattga tgatccaatt tttttccttc cggtgaggtt 17640
caaacatttg atgcttagtt tcatgagggt attctgtgtt tcgggttgtg atatgcacaa 17700
ttactcccag tttatgcttt gatgctgagt ttttatttct cttcttacac gtgcactctt 17760
catttccatt tcattcaaaa cagaaaccaa gttgattgca ttgtggaggg gaatatgaga 17820
tcagaaatca aatggttagt tgtggttttc ttatttcgtt tgctatgcgc agttgcgcac 17880
caaccgtttg ctagaatgtc tgaaagagcc tatgtacata tggtggcctg aacattacaa 17940
gttatcatat tttatattgt tgctagcttt cctttcaaaa aaaaaaaaaa ttgttgctaa 18000
ccgatcacat agtccagtag tccagtagta agatttttgt taagtttatt gttactgaat 18060
atattgtttg gcctgcagtt gttatttctc tcaaaacaaa attatttggt agtctcaagt 18120
acaaaaagaa agacatatca gacaagttgt tttactctac tagtttcaaa ttgatcatct 18180
ctgtttgttc ttcattcatt ttctttctgt aagagagttt gctaggatgg tgatgtggtc 18240
atgtggatga tcaaatggac tacatcagac gcatcacact gctgcccaac ctttacccac 18300
tgtagacaaa tggagtgcag gtcctaaacc aggccagaag tttgttcagt gttcttgttc 18360
caaaataaac attctggatg gcaggttatt tcattataac attcactctt atagctttct 18420
tagtcaaaac tacaaataag gtctcctaaa aaatgcatcg acgttgatat ctgtgttttc 18480
tgccatgcag aatgacttgc tctcaatggt tgaagctgca atcctccatg ctcatttctg 18540
gctgaaccca aattggtggc ttggaggctg gagctgcatg acattagaga taacaatggc 18600
cactttttgt tgccatgggt gagggtacat ggatcatgcc gctgagcctc tactccgagc 18660
aagcagaaaa ctggctggct gtagatcgag ttcgccatcg ccctcaactt tgtcgatgcg 18720
atggtgatca tggatagatg tatgtcacat agcgcaaagc ggagcctcca atgttcgcaa 18780
ccgtactgta aatgtgggag cggcgaattt ctcaagggga caaccatgga acagaggaga 18840
tggagcaaag tagttgctct tgctatcatt tcagagctca ggctgatctc tagctcagat 18900
aggagtacga attcttatgt gtgtgtattt gatctatgat agtacgtttt aagagttgag 18960
ccagatattg cttgatcatt acatgatgat atgtaaaaaa atggtattga caaggagacg 19020
caggaacagg gtggttcatt gctctgttta atcttaatct tggagagcta ggatggaaaa 19080
ctgagttggt ggttatatct ctactacttt tgatgttgtc ccaaactaat ttgtttctac 19140
tcatgttcgt acctaaaaaa ggaatatttt agtgattaca gaacttaatt ttctctttaa 19200
tcagattatt tatcagtggg atttttcttt ctctagttct gtatgaaaat acttttttat 19260
cgtcaatcct cctaaaattt tgtgatatca gtattttttt tattttactg gaatgggctg 19320
tttcggtgct gcttgcttgg acttgctgat tcctccctct ttcctattta taaactcatt 19380
tttcttcagt tttttttcct gtattttggt tttctttcct ctacgtgact acacattttg 19440
aatcgaacat gctatgctct gtatatctgc ttggaatact tattaaatgc ataggccggc 19500
catttggaat gtgcacttaa cagttgtttg aacacttcca tggatttgtt tcctcagttg 19560
tcggacggct atttaaacct gattaagaat tccatgtgca gagacttgta ctagcgtcga 19620
agactttgct tcggtgactc ggagtcaagt caaggccggc acagcgcaag ccaataagct 19680
tcgacgacga cgacgacaac catgccgcgc tattgcttgt aaactttgtc tacaaaagcc 19740
agccgcgatg ccatccatcc attccttcct tccccattgt tgctcagcca ctcggcgtcg 19800
gattttcctc taccacacca gctctaccaa cctttcccga gattagagag gaagaggagg 19860
acgccgaagc cgttgcatca agcccaggac caccgtcgaa cacctgctgt gcgcaccgct 19920
cggcattcct tcgcttcacc accgtgtgtg cacagttccc accagagtgt gcaaagcagc 19980
ataggtaagt caactccgat tttctgctgt tctttttttt ttttaagata aagcaggagt 20040
tctgctattc aatgtcacgc ccggaaattc actagtaatt tccgaactta tttgtgcata 20100
aaatcctcgt ccaggaatca gccgaggtac acaaactgac aatttaatat acaaattcat 20160
caaattaact aaacgataaa tacttactta tgaggcactt agtcctcatc atgaagaaaa 20220
ctacagcgga aaataaaatc tagcgaagct ccggctccac tcccacaggc agctcaactg 20280
gggtataagc caaacgtctt ctccttctga atcctttttc ttcaactgag gttgattgat 20340
tattgcaagg tgagcatatg acatactcag caagccacac agcaaatatg caagtgcaca 20400
aggataccaa aggatggcat aatataggct catttgcaaa agcagcattt agcaaaacat 20460
ttaagagaag taaaacagtg gagtaattaa tcagaaattt taatcaacac tgaacaacac 20520
gcccatgctg ctcaggccca accattctga agcaaccata cccggctgta cagatctaat 20580
ctccaaacca ggagctaagc aaattattac caggtataac atctataatt attgtgagag 20640
gtgtgagact aatcacgaaa aacattgctc aactcgccca taaccgcggg cacggctatt 20700
tgaatagttt tactctggcc agaggtgtac cactgtaccc acaagacaca gccccacatc 20760
atgtcaccat gtgcctcagt accaccacgg tacctcggaa aggggctgtg acagtacccc 20820
ttgcataaca caatccatcg cagcgcacca ttcctggatc ataatcaccc ccttataaac 20880
aaggcatgga ctccccagcg acccccgtgg gcttatctcc gccacttctc agtctggtgc 20940
tttacaatga accatgctat acaaaaggta aagccgttgc ccacgctggc ttgtggttgg 21000
cacgattaat gtttcacaac agtagctcgc gaaccggtcc ttaattgtca tgagcacgac 21060
tctcaaaacc atgtgctcac aacccaccat tatcaagttt tggttggcaa gtaattaatt 21120
aaccaatcac gattgaccat cgtgagctat cattaaatat aaccataagt gatagtgaaa 21180
cattagttat cccaattgtg tgctaatgtt tctaagcatg gctaagcaat tatatctaat 21240
atctagttaa accaatatat aaagctcaac tagtcaagtt ataataaccc aaggtatcaa 21300
ggaataaagt aatcaatgca aacaggccat aacaaaggaa taggttcaca ccacccagtg 21360
acattcgaaa ataaatgcac tgttgaaata aatagagaat ttaaatatag gatcaacatg 21420
ctcaaaggat tgtgtttagg atctgtgtga cttgccttgc aataatcggt cttcaattaa 21480
tcttcttgaa tacttccggc gcactcgcga accttcgcaa cgacggaaac gacaagctaa 21540
cacgcaaaac gaagaaaaag actaataaaa accaaataaa caatacataa aaaataaaca 21600
aacatgtaga tcataatttt agatgaatta tgagacttga acggcctcat tctgacttca 21660
aatgaattta ttatgaattt tacaagatta aatctaatta aagcccattt aaaaagaatt 21720
aaataaattt aattcaattt atggacaatt ttaatatgta gatctttatt ttacacaact 21780
tttgcaactt gaaccacatt aaactgagtt caaatgaatt agttatgaat ttttaaagat 21840
taaatcggat taaaacactt aaattgattt taattgaatt atgacgcaat aatgaattat 21900
ttttgaaaag gaaaaggagg attattgcgt cagcggctag ggtttgcggt ggaccgggtg 21960
cacggcgacg gttcacggga acggacggcc gagatcgact tgatccaaaa cagacggccg 22020
agatcgatcg gtccacgacc ggctcacggg agagggcgac gatgacgtca gtgacgacgt 22080
cacctccggc ggcgcttcgg cacgaacgga ggataccaac gggtagagcg cggcgcggcg 22140
aactcaccgg taaccaaaac ggcggcggaa aagcaacgga cggcgccggc gacgaggaag 22200
aagcggcagc ggagatcggg tcgacggcgg cgacggtgct tcggcggtct tcggcggcgg 22260
cgaaacggtg gacggggaag gcggcgactt ggcgatcccg acggtggcct tcccgagcga 22320
cggcgacgac tggagcgacg gcggcgcacg gctggagcgg cggcggcgac ggcggcgcta 22380
gggcacacgg cgctagggcg ctacggacga cgagaggcga aggcgagggt ggcgacgggt 22440
agaggagagc ttgggggtcc ttttaaaggg gctcggaggc gacggcgaag gcccacgacg 22500
gccagcgacg agaaggaaac ttcgggctcg gagagagaaa agccgatccg attcgacctc 22560
gaatcaaaca cttcccaagc cgatttagcc gatgtttcca aagaggaaaa ggaagaggag 22620
atcccggaga ttgtttcccc tcaatcaatt cggccggaga aggaaaggag cggccggatt 22680
tggaaaggaa cggcggcggc gcaaagctag ggtttcgggc ggcggcggcg cgaggaggac 22740
gacgggtccg acagacgggc cccacctgtc agcggctgag agagagagga gagcggcggc 22800
tcggcggact gggccgactg ggccggggag ggagagagaa agagagaggt tttgggccgg 22860
ctttcggccc aaagccaaaa gaagactttt aaaaaccttt ttcaatttaa attattcatg 22920
aaatgcaatt ccatttatta aaaatacttc cttagctcaa ataaatccca gaaaaatcta 22980
ggaattatag aattaagtaa agtatttaat aaaattttat ctagcccaat tttatgttga 23040
gatttagcaa attaaaatta gatcttctct tctaggcttt taaaatcatt tctaataatt 23100
ccttttaaac aacaatttat aatttaagga ttttaaacaa gaaaagcact taacaaatat 23160
aattagatca ttaatgatca aataattact gaactgttct ttgtattatt taagaattga 23220
gctctgaaaa tccgagaaaa tttcagagag tataattaac cattagtttg agcttccaat 23280
ttgtacatat ttgtgattta taatgctata tatacatgta tacagacaag taacgcagtc 23340
cacgtgggat gttggccatg cctggataag ctgagcaaga tttagacctg tgtcaaaaca 23400
atgtcttgaa ttccgttgaa ctttttgttt acaggttcat caaagatgag ctacaaacga 23460
tacaagcatt tttgattgct gccgaagcat caaagaaaag catactattg aaggtttggg 23520
tgcagcaagt aagggatctt tcctatgaca tcgaagattg ccttgatgaa tttacagttc 23580
atgtgggcag ccaaaacttg tcgaggcagt tgatgaagct aaaggatcgc catcggattg 23640
ccatccagat ccgcaatctc aggacaagaa ttgaagaagt aagcactagg aacatacgct 23700
acaacttaat agagaatgac ctcacctgca ccactactga tgagaggaat ttatttatgg 23760
aagacattcg caaccaatca gctaacaaca ttgaggaagc tgatcttgtg ggtttttctg 23820
gacccaaaag agagttgctt gatcttatag atgtccatgc caatgacgga cctacaaaag 23880
ttgtatgtgt tgtcggtatg ggtggtttgg gtaagactac tattgcaagg aaaatttatg 23940
aaagcaaaga ggacattgca aagaattttt cttgctgtgc ttggattact gtttcacagt 24000
cctttgttag ggtggaacta ctcaaggatt tgatggtgaa actttttgga gaggaagtac 24060
tgaagaagcg gctgagagaa ctcgaaggga aggttccaca agtagacgac ctctccagct 24120
acctcaggac agagttaaat gaaaggaggt actttgttgt gcttgataac gtgtggagta 24180
cagattcatg gaaatggatt aatagtattg ccttccctag aaataacaat aaagggagcc 24240
gggtgatagt aacaacaaga gatgttggct taactaagga atgtacttct gaattgctta 24300
tctaccagct taaaccccta gaaataaact atgcaaaaga gttgcttcta cggaaagcaa 24360
ataaagcaat aggagatatg gaaagtgata aaaagatgag tgacattata actaaaataa 24420
taaagaagtg tggctattta ccgctggcta tactcacaat aggaggcgtg ctttccacca 24480
aagagataag agagtgggaa actttttata gtcagatatc ttcagagctt gagagcaacc 24540
caaaccttga agcaatgaga aggatagtga ccctaagtta caactactta ccgtctcatc 24600
ttaagcaatg ctttttgtat ctaagcatat ttcctgagga ttttgaaatt aataggaacc 24660
gtctggtaaa tagatggatt gcagaggggt ttattaaagc taggactaat atgactattg 24720
aagatgttgg gaaaagttac tttaaagaac ttatcaaccg tagcatgatt cagccatcaa 24780
gagcgggtat acgaggagat tttaagagct gtcgagtcca tgacatcatg cgtgatatta 24840
caatttcgat ttctagagaa gaaaatttca cactcttacc cgatggcact gactatgatg 24900
tagtacatgg gaacactcgg cacatagcat ttcacgggag taggtattgc tctgaaacaa 24960
gcttggactg gagcattata cggtcattaa ctatgtttgg tgagaggtcc gtagaactag 25020
agcattcagt ttgttcatct cagttgagga tgttacgggt cttggatcta acagatgcac 25080
aattttctat cacacaaaat gatgtcgaca acatagtgct cttgtgccac ttgaaatacc 25140
tacgcattgc aagatacaga taccgttcac catatattta ttcacttcca caatccatag 25200
ctagactgca tggtctgcag acattggact tgggtcagac gtacatttca acactgccaa 25260
ctcagattac taacttcgga gtctccgtag ccttcgatgc atgaaagaat atttttcttc 25320
tttaacaaca tatttaacta acacattatg cctgcccatg atattcacac ctttcgttag 25380
tacctcggat cgttctgaaa caattgctaa attgcacatg gccaccaaag gcttccgttc 25440
aaaatcaaat ggtgtcaagg taccaaaagg aatatgtaag ttgagagact tacaagagga 25500
ttgctacggt ccagcaggtt gtaccgggcg gtactggtac cgcgcggtac caaaacccat 25560
ctaaccgttg aattcgggat gggtaggatc gggagagaaa agatgagcaa gggtggatga 25620
gggagtacct gtttcgagtc gtcgttcccg gcggcggcgg cgtggagtac ctgtttcgag 25680
tcgtcgtcat tcccggtggc ggcgcagagc aacaagggac gccggcggcg cgggagagga 25740
taaagtccgg cggcggcgcg agagagaaaa aagggaacgg cgacggtgtg ggagaggaac 25800
aagggaagga cggcggcggc ggaagaggaa caagtccgac ggcgaggaag aggaacacgg 25860
cggcggcgaa aatcatccag cgtagctagg gttcgagccg cccgatccaa acccatctat 25920
tgcacgcgaa gttactcttt tacccttcca actctcttct ccatgcggta tcacctaagg 25980
gacatttttg gtaccgtgcg gtaccacgca acatcagccg ttgtatcagg ccagatccaa 26040
cggccagcat ttggtaccgc tcggtacgtt ggacagtaaa aaaaactcga cttacaaata 26100
ttggaggtag tggatattag aaggactagc agtagagcaa tcaaagagtt ggggcagtta 26160
agcaagctga ggaaattatg tgtggtaaca aagggatcca caaaggaaaa atgtgagata 26220
ctctatacag ctatccagaa gctctgtttc ctacaatctc tccatgtgaa tgctgtggga 26280
ttttcaggta ttggaacact tcagtgtata gattctattt catctcctcc tcccctactg 26340
aggacactca ggttgaatgg aagtcttgag gagatgccta actggattga gcagctcacg 26400
cacctgatga agttcaactt atggaggagc aaactaaaag aaggtaaaac catgttggta 26460
cttgcggcgt tgcccaacct catggtcctt tatcttcatt ccaatgctta ccatggggag 26520
aagctagtat tcaaaatggg agcattccca aatcttagaa cattttcgat ttacaatttg 26580
gagcagctaa gagagattag atttgaggac ggcagctcaa tcttgttgga aaagatagaa 26640
atattcaggg gttggaatca gggattgttg gtatcattca ccttccaagg ctcaaggaga 26700
tttcacttgg atacggaagt aaagtggcta ggcttggtca gctggaggga gaagtgcgca 26760
cacacccaaa tcaccccgtg ctgcgaatga gggaggaccg aagtgatcac gaccttgctt 26820
gtgacgccga aggatcccct gttgaagtgg aagcaacaga tcctgtgaga gctcgcagtt 26880
gcaggtgatc acgttgacaa cgaacgacag gtcagtcact ccctacacgg catcttaatg 26940
aacttgtttt atcctcttgt gagatcgatg attttaactc accctttcat ctctctcgtt 27000
ttcttaacct aacagcgaag agataagcac aacttaagct ggtttgatca agtgatgatc 27060
tcctcctcca ttggcatctc cggtcgtccc tgcttctgcg gctgcgcacc tcgctgctcc 27120
gaggaggggt gctgatctaa ggaggcttcc actttcttca attgcgtctc atgctctcga 27180
ttcttccctc tcgggtatga attgttcaat ctgatatttt ctcgcgatct gctactggtt 27240
ccagcatgag catttgaacc agcagcttag aattatcgtt tgatcaggtg ttatttatcc 27300
cttcttacct gggaactcta cttatccatt tcattcagaa cagaaaccat gtttattaca 27360
ctatagaggg gaacaacaga tcaggcacga gttgtggttt tgttatttcc tttttggtgt 27420
gcacaccagg tgattgctag aatgtctgaa agagcttgtg tgcatggttg gctcaacatt 27480
atgtgctaat cactctttta taccgttgct gacctatcac atagtgcagg agtaaaattt 27540
tgctatgttt attgttactt aatgttgatt ttccttttcg taaagaatat tagcttttta 27600
gataacaaaa agaatattat gtatcctgca aaaaggtttg atttggtgat cactggctag 27660
tacaaaagta acgagagatc agaaaaaaca aagttttggt tacactagct cattacaatt 27720
tatttgctga acatgtgaaa aattaataat tgtcgtacgt catggttctc tggaagtctg 27780
gctgcaggtg gttagttttg tcatatccat tatcttggtc taccttttcc tactaaacta 27840
ggactatgag tagaaaaaag taattgttgg ttccaagttc aaaagatagg cagatgtgag 27900
ctaaggaact tacagttctt gatctccttc agagcttctc acctcacata aatggatctc 27960
tgtttatccc aaagcaacat tttttaacct gctagttcca aattgatcat ctttgttatt 28020
cttcatttat tctatttctc tccctaaggg agttttctag ggtggatatg tggaaaatcg 28080
aatgcagcta tgctgttcaa accactgtca aaagatgcag gacaggatgg gcagatgttt 28140
attcagtgtt cttgttcaaa tgaaacatat gctattctgg aagaggttgg ttaacttaca 28200
ttgtatataa cccttacctt gttactatcc tcttgcaaaa tgcatggatt ggtgaaatca 28260
tgttttttgt catgcagaat tgttttctga agctcaaagt tgaagctgca gtcttcactg 28320
tttctggcag aacccaaatt ggtggcttgg tgctgcataa cctgcagaga acgcaacggc 28380
cactgcttgt agccttcaag gatgctacat tattgatcat ctcactacgg tcccgatcaa 28440
acttctgatc gagttcgcca ccgctgtgga ctttgcatat gttgatggag tggtgcgtat 28500
gaatccaggg ggcagagcca ctgccctgcg accttggggc ttgtcaacga gcatacagta 28560
taatattttg ctgttatttc agtgattaaa aacgaaaatt ttaaagaaaa cattgaatgc 28620
aaattagatc tgcccaggtt ctcaaaattt ctttagctcc gccactgctt gaatcgatgt 28680
catatgctgc aaattaagtg gattggagat gtgcatatgc tcgaatgttc tcggttatat 28740
tgtaattgtg gggtagcaac tttatcttgg gtacaaccat ggaacaaggg tgaaattgta 28800
ctaattctta tttgtgtaca attgatatat ctcattactt cttgcattct gttagtcata 28860
tgtatttcca tacatcgttt gcacctgcta tggctgcttg aggatatggc aaagcttaaa 28920
agatgatgtt aacatggtca catggagatg cagggcactc cattttctgt ttttctctca 28980
atcttatgta gagttaatat atggacagta gcaatagtta catctttctg tcaactaggc 29040
atactaccca cgtgttgctc cgggtctttt tgggatggtc tctattaaga tgtaaactta 29100
tttattaaat gaattcaatc gcgtcgcatt ggttcattta tttagacttg gttgtgatat 29160
actcctgttg taaaatataa taacttttac gattctagca ccatttataa tatttataag 29220
agtacctgtc tcagtaatca tcaatcattt tctatttagt tctttctatc ttacccttac 29280
atacctttca acactcatcc attcctctgg tgaataacta aaaatgttta aatttcagag 29340
cggaggtagc aataagttct agtaaaagct gttgaatagt cccacattgg ttgtaaaagg 29400
acaaatgacc taacatataa gtgggtgagc cctgtacctc attagctagc tttttgggtg 29460
aggtcccttt acgatcttat aattggtatt agagcctggc tagtttgaca tttacccgag 29520
ggcactacta gaaaaaggac cgaccgccgc tagaccaaca aaggaacata gacgagatcg 29580
ccccaaaaaa agcctccaca accaacacaa agcccaactc ctaaagcgtg cttgcaccaa 29640
tcgttcgaga gatttcggct aggggatgcc aaaatgacgt cttcgagaaa agaagcgatg 29700
gaaaaccgcc gccgccgtct gtcggggctc aaaggagcca agactgggct ttcgcccggc 29760
aaccaccctt gagggataag acatcacgac aacgccctca ggaagtcagg agggggaatt 29820
aaccatcgtt gtcggtccgg ccaaggccgg gctgggtttt cacctgccgc tcaccacctg 29880
cgaattcacg gctgacgcac cgatgctcca ccaccactca acctctgccg ccatgtggga 29940
ccactgcact ggcgccccct gtcagccaac cttcatgcgc cgaagaccgt gccacaccca 30000
ccgacagctc ctcctcgcac tgagactgcc tcctccacta ccgcccaagc ctctcgcgcc 30060
aagccggcct tctctactgg acgcgcctct cgcgccaatc caaccttcct ccattggccg 30120
cgcctctcgc gccaagccgg cctccatctc ctccgcccgc gcctctcgcg ccgagccagc 30180
ctccgctgcc agcagttgcg cctccctgca ccaagccggc tttcgacccc tcctccaaag 30240
gctaccgcac cgaccggata cggccgtctg ccacgccccc ggctagccgt ccgagaccgc 30300
catgcctccc cctatggcgg tggcgatcgc caccaactag ggttgaaagt gattcggata 30360
atttccgtcc gaccggacct ttttacggat tcggatagtg tcggtcggat atattcggaa 30420
atttgaattt gaaatcatga caacttcaaa tagcattttt aaatactaaa tgatttcaac 30480
tgaaaaagtc atcaacaaca aagttgtata actcatcaag atttataact ttttattttg 30540
gtcatttctt catccgacaa agtgatagta atattgttca caaaatttac atctctcatt 30600
aggttttatg aactataaga gagatatata aattttatga acaatgttac tattactttg 30660
tagaacataa aagttgtaga actcatcaag aaatacaact tttattttgg tcatttttct 30720
aaaagtttga atttgaattt gaaaatatga caacttcaaa taatattttc aaatacttaa 30780
tgatttcaac tgaaaagtca tcaacaacaa agttgtataa cctatcaaga tctataactt 30840
ttattttagt cattttcttc gtaagacaaa ttgacactaa cattgttcac aaaatttaca 30900
tctcttattt ggttttatga actataagag agatatataa attttgtgaa caatattact 30960
atcactttat cagatgaaga aatgaccaaa ataaaaagtt gtattccttg atgagttcta 31020
caacttttta ttgtcagact ttttcagctg aaattattta ctgcttcaaa atatcatttg 31080
aagttttgaa attcaacttt ttaattgata aaacaaagtc acaagaaaaa atggccaaaa 31140
taatagcagt aaaaaacaca ataacatgat agagcatgat tttagaaaca tttaggaaaa 31200
agaatcatcc aatttggagt tcatatgagt gagataaact agtttcaaat ttttaaattt 31260
tattttcgca tacggctcct taagacgtcc gtatggaaaa attgattttt ccacgcgggc 31320
tcttaagttg tccgcacaca aaatgagctc attttggcgt cttgaggagt cgtatgcgaa 31380
aatgtcgacg cggcaagttg tgatccgttt ggaaaaatca tagggtctcg tacaaaagaa 31440
attgtttgtg tagtagcgag gggtttttat attccgatta atattcatca ccgtattcgt 31500
ttcgctccgt atttgtattc gataatattc catttcgttt ttatatccgg gtttccagtt 31560
ccgaaaaaaa aagaaagtga atacgataga gctagtttcc gaccatattc gatccgtttt 31620
catccctacc accaccgcag ccgctactgc ccttccatcc ccgccgccat cttgccatcc 31680
tcccgcacct tctcgcagtc atcgagctcc gacggcacac ggcgcagacg gcccaccgtg 31740
gtagcccgca ccgccgtcgc cacgaactcc tcgccaccac cgcctcgacc gccagactcc 31800
ttcgggcgct gggtctgccg tcggcgcggc caggttcgcc tcaccgacgc catcccctcg 31860
ccacccccac cgcacgacgc tgccgaaggc tgccatcccc tccccttgcc tcccctgccg 31920
ccatcgccat ccccgccacc agacggcgcc gccggccacc atcccgccag atccaggtgc 31980
ggatctggcg gtttcctccg tcgccgtaaa cgcctcgaac gccgctgcca ccaccaccag 32040
acaccaccgc cgcaccgctc agccccgctg ccagctgccc catcgccaga tccggcgggg 32100
cggcacagat ctgggctgtt ctgctgcccc gagcaggccc ccctcctatg cccgagcacg 32160
aggatgaagc cccgccgcca ctgtctttgt ggccgcgcga ctttgccggc gactgctagg 32220
gcagcggcga ggcagaggag ggaaagggag atgagcaccg gcgaggtcgt cgcctcccag 32280
ttgcccgtgg ggaggggcga cacgagaggc caagcgctac tcaactgcct gatgctcatc 32340
caaagtgaga aagatgcttg aagctgtcgc tcgaagcaac tttcagtcct cgatataatt 32400
cgatataagt gatttctctc tccatatttg tttggagaaa tgctagttat aagaaactaa 32460
gtgtgaccat gtgttataga tgtcagagaa aacagttcat tttctcaatt ctcaaggtaa 32520
ttgggaaaat ggagaattac tgagcgatac gtgttgctgg aaaattgaga atcactgatg 32580
atcgcctcca tctgaaaatt accgagcgat atttattgct gccaaatcaa aatgattcta 32640
ttcaacccgt gccatggcat ggccggatgg ccccagttca ctgattgatc acttaatata 32700
tggactgaac aataaatcat ggtactttgg ttgtcgagca tgatcattat ttgtggacca 32760
cagattcacc gaattaattg ggatattgaa tcggtcacac acacgacggg cgtactccgc 32820
tcgtcttaaa ataaatggat tggacgtgac attatctact acaacgaatt tggattagat 32880
agtgtctcat ccaatcctaa attggtttat tttgtgacgg agggagtatg atgttaacgt 32940
agatggaaat gaggaattga gtagacagtg tggggctgga aaataatgga ggacagtaac 33000
atcttggagt gtagtgaggc ctggagggtc gtccttgaca tccaaaccgc acctaactct 33060
atgataagca tcctctctca gattgttcag tgcaaaagct accaatactg ctccgagagc 33120
cagaaaaaag cgctggtcgc ctagtgctat cttctatgca gtcgtgagat tggttgctct 33180
aagcttgaag ggagagtcga acgagtccat ggcggagacg gtgctgagca tggcgaggtc 33240
gctggtgggc agcgccatca gcaaggacgc ctccgctgct gccgacgaga ccagcctcct 33300
gctgggcgtc gagaaagaca tctggtacgt actgcgtgac tctcgttaat ttattctgta 33360
gatgctcagg aatcagcaac tattgtgttg atttccatcg tagcatatcg attttgttgg 33420
ccaccaattc taatcggccg gaacaagcta gtcactaaat ctggcaaatc gatcagctgc 33480
tgagtgcaca aacatgcatg ttattctttt tttttttggg ttatatgtta agcaacaaag 33540
ccccttggta agatatgcat ggcaaatgaa ctaatatcga catacgtaaa gcggaggacc 33600
cctcgttcca tgcgtgggtg actcgagcgg tgacaaatcc tagcacctcc acctccttgg 33660
atggcctgtg gtgacgcttt cggccccgag tttccattga atacatcatc tacaaggtgc 33720
tattaatgta tagtcacatc atttacgagg cgctattatt gcctagtctg cccgaagata 33780
gtttagacaa cactcttgga tggcggtgtc cttcgcccag tgatgtccaa gagcccgtgg 33840
atgtttagtt gtttagacat ggtgttgggt ggtgcactag tgggcctgat gggccagttg 33900
taatagccgg atgcccggat tggtgcttgt tcttttttcg gtgtcgacgc atggtagtat 33960
ttacttttcc tgtttttcct gattatagca tcctaggcta tactcttcta atttattcat 34020
gctatattaa tattaaaact tggtatggtt tgtttcattc aagacccttg gtggtcaaag 34080
gcttgtttgg ttcaagttca ttcctagcct taccaacttt ttggcaatag caagaaatgg 34140
tcattgaaaa aaaaaggcaa aaattggcta ggcctacagt ttatttccta gcaaagttat 34200
actttagcat tccactaagc caaataattc ggcaatgcca ttttcttatc tacatgccaa 34260
atatatggct aatattttgg cattaattac tcttattttt tttggcaaaa ttgatcaaaa 34320
gttcacattt ttagctctat agtatcaaaa gttatctatt cactttaata gaccgaaagt 34380
ttactcggtt ccgtttttag cactaccgtc tcttttctct tgatttgccg tcaattttga 34440
ccggcagtcc tacccccagg agacattgag cagcagcccg tgatccccct ctctcgccgc 34500
cggtgacgct gtggtggcat cgttcctgct gcgggcagaa taagtctggc gtcatcgccc 34560
tatcgcctgg agctgcaacc accactaccg ggcccatcga tcgtctagag cgttatccac 34620
cctgcctgcc ccattacttg cagctccggc tgggtcagaa cctctccatg cctgacaaat 34680
tggttcaaga ttgtcgctgt ccggccagcg cttgaatttt cagaatatgc catcgaatac 34740
gcgctgcttt taagatatgc tacccgattc atgctatttt tagaatacgc catcggaaca 34800
cgaattttct tcgttccgtg ccactccgtc tctcggagtc agtcgtgccg tcgtcatccg 34860
tccgcccagc actgtcgtcg tcagtccgcc acccgtgcct gactgcccgt tcagctgcgc 34920
cgtcgtccgt ccgtcgccgc catcgtcgtc gtccactgtc gcgcccgcac ctgcacccgt 34980
gtcaggcgcg ccgtcgtccg tccgctgtca ccatcatcgt cgtccactgc ggcgagcgca 35040
gacggctgtg gacggatgat tgcgcggcaa gcgcaagcgg cggtggacgg acgacggggc 35100
agtgcacgcg agcacggtag ccgatggact gacgttggcg gcattgggag atggacgacg 35160
acggcatgat tgacgtgggg aacggaatgt cacggaacgg agaaaattcg tactccggtg 35220
gcgtatattc cgaaaatagc acaattgggt ggcatatcct aaaaacagtg tctattcggt 35280
ggcatattct aaaaattctc gatcagcata atccccatca atccccaatc cctcaacagt 35340
tgggttaata ttcctggaga tgtgttcggt tgtttaggtt gaagttctcc acttcacctc 35400
catgagtaca tgcacctcta cacgtacgtt cttaatgagt ttgtttgttc tatcctccgc 35460
ttgggttcta ttttgttggt tccgatctga tttgatctgg agcggggtcg atcttccacg 35520
acggcgagag agacgttgtt cgggctgctc gatttggttc aactgtttag gtcgaaggga 35580
ggggtagaat tgcaattcaa gtgcatggtc agtcaatttg ggtcaaaatt aacatcaaac 35640
tgggataaag agacgacagt gccaaaattg gtaacgggaa aactttgagt tctattaaag 35700
tgaaccagta actttcgttg ctatagaata aaaacgtaaa cttttgatgc tccttggttg 35760
agcttggtac aaaccaaaca gacgtaaaat aaacactatc ctgaatcaag tctactaagt 35820
tccattgaac tcaaccagga tacgtacact tcctcttaga agatgtcttg ttttcacttt 35880
gtacaatttt ttctattgta aatttggtac ctcgttgtac ctaggtacaa gaggtaccat 35940
gagataccaa attttacact aaaattttgg tacctcatgg tacctcctca acgaccgtag 36000
aattgctctt aatttaattt aaaaaaaaca taatattttt aaagcatatt atggaaattt 36060
tagtaattat tacttttgta atatatgagt tacggttata ctcgagatat cctaaattgc 36120
ttggagatga atattgcaca tggtgctgga tggtgcgctc gtgggcctgt tgtaggtctg 36180
gtgccaacca gtcatgctta gaaatagccg gataggtgca cagtgctagt tctttacttg 36240
gtggtttgtg cagcgctatc gacatgtggt ggtgtgcttt ttctttgtcc ggataataat 36300
ctcatagggc tatactcttg ttattttgct gctatattat tatgataact tggtatggtt 36360
cgttttttct ttttttggaa aaacacctag ttgatcaagg gcttgtttgg ttcaagtgca 36420
ttcctaatct taccttttct tttttttttc aatggcaaga attgttcatt gcaaaaaaaa 36480
aaagagataa aaattggcta ggcttacgtt ttggttctta ccaaagttgt actttgagac 36540
caaatatatg gcaaaatttt ggcataacct tttttttttt tgcttggttg agcttggtac 36600
aaaccaatca gtcacaaaat agactgtcat gaatcacgcc tactaaattc ctttgaaccg 36660
aactagaata tatttgctct taaaagattt cttgatttca attggtacca tttactagta 36720
gaaacttaaa tttaaatttt aaaaacaaaa tcataatatt gttgttatgg aaattttagt 36780
cattttagta cttttgtaat atatgagttg ggttatactt gagatatcct aaattgcttt 36840
aagatgaaca attgctaggt atatcaaaga tgagctaaaa acaatgcagg cattccttag 36900
agctgctgaa gttatgaaaa agaaagatga actattaaag gtttgggcag agcaaatacg 36960
tgacctgtcg tatgacattg aagattccct tgatgaattt aaagtccata ttgaaagcca 37020
aaccctattt cgtcagttgg tgaaacttag agagcgccac cggatcgcta tccgtatcca 37080
caacctcaaa tcaagagttg aagaagtgag tagcaggaac acacgctaca atttagtcga 37140
gcctatttcc tccggcacag aggatgacat ggattcctat gcagaagaca ttcgcaatca 37200
ctcagctcga aatgtggatg aagctgagct tgttgggttt tctgactcca agaaaaggct 37260
gcttgaaatg atcgatacca atgctaatga tggtccggcc aaggtaatct gtgttgttgg 37320
gatgggtggt ttaggcaaga cagctctttc gaggaagatc tttgaaagcg aagaagacat 37380
taggaagaac ttcccttgca ttgcttggat tacagtgtca caatcatttc acaggattga 37440
gctacttaaa gatatgatac gccaacttct tggccccagt tctctggatc aactcttgca 37500
agaattgcaa gggaaggtgg tggtgcaagt acatcatctt tctgagtacc tgatagaaga 37560
gctcaaggag aagaggtact ttgttattct agatgatcta tggattttac atgattggaa 37620
ttggataaat gaaattgcat ttcctaagaa caataagaag ggcagtcgaa tagtaataac 37680
cactcggaat gttgatctag cggagaagtg tgccacagcc tcactggtgt accaccttga 37740
tttcttgcag atgaacgatg ccataacatt gctactgaga aaaacaaata aaaatcatga 37800
agacatggaa tcaaataaaa atatgcaaaa gatggttgaa cgaattgtaa ataaatgtgg 37860
tcgtctacca ttagcaatac ttacaatagg agctgtgctt gcaactaaac atgtgtcaga 37920
atgggagaaa ttctatgaac aacttccttc agaactagaa ataaacccaa gcctggaagc 37980
tttgaggaga atggtgaccc taggttacaa ccacctacca tcccatctga aaccatgctt 38040
tttgtatcta agtatctttc ctgaggattt tgaaatcaaa aggaatcgtc tagtaggtag 38100
atggatagca gaagggtttg ttagaccaaa ggttgggatg acgactaagg atgtcggaga 38160
aagttacttt aatgagctaa tcaaccgaag tatgattcaa cgatcaagag tgggcatagc 38220
aggaaaaatt aagacttgtc gaatccatga tatcatccgt gatatcacag tttcaatctc 38280
gagacaggaa aattttgtat tattaccaat gggagatggc tctgatttag ttcaggaaaa 38340
cactcgccac atagcattcc atgggagtat gtcctgcaaa acaggattgg attggagcat 38400
tattcgatca ttagctattt ttggtgacag acccaagagt ctagcacatg cagtttgtcc 38460
agatcaattg aggatgttac gggtcttgga tcttgaagat gtgacattct taatcactca 38520
aaaagatttc gaccgtattg cattgttgtg ccacttgaaa tacttgagta ttggatattc 38580
gtcatccata tattcacttc ccagatccat tggtaaacta cagggcctac aaactttgaa 38640
catgctgaga acatacattg cagcactacc aagtgagatc agtaaactcc aatgtctgca 38700
tactcttcgt tgtataggac agtttcctta tgacaacttt agtctaaacc acccaatgaa 38760
gtgcataact aacacaatat gcctgcctaa agtattcaca cctttagtta gtcgcgatga 38820
tcgtgcaaaa caaattgctg aattgcacat ggccaccaaa agttgctggt ctgaatcatt 38880
cggtgtgaaa gtacccaaag gaataggtaa gttgcgagac ttgcaggttc tagagtatgt 38940
agatatcagg cggaccagta gtagagcaat caaagagctg gggcagttaa gcaagttgag 39000
gaaattaggt gtgataacaa aaggctcgac aaaggaaaaa tgtaagatac tttatgcagc 39060
cattgagaag ctctcttccc tccaatctct ctatgtgaat gctgcgttat tatcagatat 39120
tgaaacactt gagtgcctag attctatttc atctcctcct cccctactga ggacactcgg 39180
gttgaatgga agtcttgaag agatgcctaa ctggattgag cagctcactc acctgaagaa 39240
gttctactta ttaggcagca aactaaagga aggtaaaacc atgctgatac ttggggcatt 39300
gcccaacctc atggtccttt atctttatgg gaatgcttac cttggggaga agctagtatt 39360
caaaacggga gcattcccaa atcttagaac acttcgtatt tacgaattgg ctcagctaag 39420
agagatgaga tttgaggatg gcagctcacc cctgttggaa aagatagaaa tctcttgctg 39480
caggttggaa tcagggatta ttggtatcat tcaccttcca aggctcaagg agatttcact 39540
tgaatacaaa agtaaagtgg ctaggcctgg tcagctggag ggagaagtga acacacaccc 39600
aaatcgcccc gtgctgcgaa tggacagtga ccgaagggat cacgacctgg gggctgaagc 39660
cgaaggatct tctatagaag tgcaaacagc agatcctgtt cctgatgccg aaggatcagt 39720
cactgtagca gtggaagcaa cggatcccct tcccgagcag gagggagaga gctcgcagtc 39780
gcaggtgatc acgttgacga cgaacgatag gtcagtcact ccctacatgg cagcttaatt 39840
aacttgtttc taattctctt cttgttcagt attagccatc aggtgagggc gatgatttca 39900
actcactttt catctctctc gttttcttaa cctgacagcg aagagatagg cacagctcaa 39960
gctggctgac gatctcctcc cccatcagcg tcgtcatcag cgaacagata gggcagggct 40020
tccctgcttc tgcgtgcacc tcaccgctct gactcggagg gacatgatga tcaatgaggc 40080
ttccagtttc caaatgtgtg gctaacacac caggttgtcc ctatccgaga tatgaattga 40140
tgatccaatt tttttccttc cggtgaggtt caaacatttg atgcttagtt tcatgagggt 40200
attctgtgtt tcgggttgtg atatgcacaa ttactcccag tttatgcttt gatgctgagt 40260
ttttatttct cttcttacac gtgcactctt catttccatt tcattcaaaa cagaaaccaa 40320
gttgattgca ttgtggaggg gaatatgaga tcagaaatca aatggttagt tgtggttttc 40380
ttatttcgtt tgctatgcgc agttgcgcac taaccgtttg ctagaatgtc tgaaagagcc 40440
tatgtacata tggtggcctg aacattacaa gttatcatat tttatattgt tgctagcttt 40500
cctttcaaaa aaaaaaaaaa aattgttgct aaccgatcac atagtccagt agtccagtag 40560
taagattttt gttaagttta ttgttactga atatattgtt tggcctgcag ttgttatttc 40620
tctcaaaaca aaattatttg gtagtctcaa gtacaaaaag aaagacatat cagacaagtt 40680
gttttactct actagtttca aattgatcat ctctgtttgt tcttcattca ttttctttct 40740
gtaagagagt ttgctaggat ggtgatgtgg tcatgtggat gatcaaatgg actacatcag 40800
acgcatcaca ctgctgccca acctttaccc actgtagaca aatggagtgc aggtcctaaa 40860
ccaggccaga agtttgttca gtgttcttgt tccaaaataa acattctgga tggcaggtta 40920
tttcattata acattcactc ttatagcttt cttagtcaaa actacaaata aggtctccta 40980
aaaaatgcat cgacgttgat atctgtgttt tctgccatgc agaatgactt gctctcaatg 41040
gttgaagctg caatcctcca tgctcatttc tggctgaacc caaattggtg gcttggagac 41100
tggagctgca tgacattaga gataacaatg gccacttttt gttgccatgg gtgagggtac 41160
atggatcatg ccgctgagcc tctactccga gcaagcagaa aactggctgg ctgtagatcg 41220
agttcgccat cgccctcaac tttgtcgatg cgatggtgat catggataga tgtatgtgag 41280
agctacacgt ggaggtgtaa gagataatgt aatagtagcc attggaaatg gtaatttttt 41340
tcgaacaaac aactatttct gtaggattaa atctatgaac tgaagtagca acatccgtca 41400
aggttaagat agaaatttct cagatagtaa tagcaacgca agagacacta attatgatta 41460
ttgttattga ccttctcttc agttagaatg acctgcattt gaggtaatcc attttttatg 41520
taagaaatgt taaaagctca gtttgttgag gtctccactt gtagatttgc ttggcaatta 41580
acgaaattgt tgggcttgta gaagaattcg cttgagcaac tgcatccacg ttcacttgtg 41640
aagaacgcac aaatgaaaaa tgagcagcat atcttgtttg aaagtttggg gattcctgaa 41700
ggaaagcgca ataaatggca aagtatagac atgcagtgac ccgagtaaca cctaaggtct 41760
cgacttcaaa tttttatagg agtgaatttc agattgtatt atttgaggga ttaagctccc 41820
aatttaaaag tctgcatata ttcggttcac cggttggata tagaggccgg gtaaaaatta 41880
ccctcaagaa aagaaaatga tggtgcatgg aatgcaaggc accaggaata gagtaataga 41940
gaaaagaatg taataacatt tcttaattgg tgagcagtga taacctgctg gatggttctt 42000
gttgaagatc catcttaatt ttatctgttt cttaggatac caatatgtct tagtgctgaa 42060
aatttctgtc aacagaaaaa aaacaacttg tagtattttg gattgaaaga tccataaaac 42120
tgaagataaa tagtcggatg gccttgatga aaaacccaat atttgacaaa ctattcaatt 42180
ttgctattac aaagagcatt ctttatactc tagccagaaa agaaatgaaa aattaagtta 42240
tatgggtgta aagtaggatc atcatatacc aaataagaat ctggatagac atataaaaaa 42300
ttagggatca atgtgtataa aaatagctag actgttgtta tggaaggaat tacttgtacc 42360
tcttgcacag catgtgtcgc aggatgggca tataataata tatagaaatc aagttaccat 42420
gaatgaatat ggggcaatta tgattaccca atgtatatac agattgctcc agctgttgag 42480
tgctaacgtt agagcaggta aaatagcagg ctatatgaca gctgtaaata tattttaaga 42540
aaataaatca ggagagagaa gagcagcggg ctacagattt gtagccagct gtagcacgaa 42600
ctttaagacg cggtgtgtct atgacaggtg gggctaggta ttaatagtgt agtatgtaac 42660
tatagtatga atgagctatt agattggcta tagatgaatt agagctagta gttggctata 42720
ctattgaact tgctcttagg ggaatatggt tgtccccctg tgcgttagtg cgtacagtga 42780
tgtgaactac tataagagca ggtacaatag tggactatta gccagttgta aatatatttt 42840
aatgagataa aagatgagag agaagagtag ctggctacag atctgtagcc agctgcagaa 42900
cggactccaa gacgtaatgt gtgtatgaca gatgggacca tatattaata gtatagtaag 42960
caactattgt ataaattggc tattagattg gctatagatt aattggagct agtaatgggc 43020
tgtactatta aacttgctct aatgataatc ctctctgctt gaattgttca gtgtaaaagc 43080
tagctacaaa ttctgattgc agagccagaa gagccagtcg cattgtccta tctcctttgc 43140
ggttgtgagc tgcttgctgt tctgagcgtg aaccgtgaag ggagatctag tcgagcagag 43200
tcgatggcgg agacggtgct gagcatggcg aggtcgctgg tggggagcgc catcagcaag 43260
gccgcctccg ccgctgccga cgagaccagc ctcctgctcg gcgtcgagaa agacatctgg 43320
tacgtaatat gtactgtggc tctcgtttat tctgtactag cttactgatc agcatttatt 43380
gtatcgacat ccttcttagc ctcgattttg ttggccatca atcctgatcg gaacaaatca 43440
ctaagtcaag caaatcaatt gatgagtgca caaacttttt ttttttgcat gaccaatctt 43500
gagttcttga ggggcgaaac agacgcaccc gaccgcagtc accctctccc tcctcccaca 43560
gtcccactcc tcccctccgc cttgccgcta cccgagcgac cgccggaaag ccaagtggct 43620
gcaaggacgg tggcggtggg gcatgctctc tctctctctc ggcgctgcaa aggagggggc 43680
accgacgcat ctaggatggt aaaggcgtct ccacgctagt cagatccggt gagtggctgg 43740
cagaaaacga ggtggaatag cggagggccg agccagtgct tggtgtggct agcaacatgg 43800
gaggctagcg atgcggtagc aagcagtggt ggtaactaca aatcttgttc tagatgatct 43860
atggatttta catgattgga attggataaa tgaaattgca tttcctaaga acaataagaa 43920
gggcagtcga atagtaataa ccactcggaa tgttgatcta gcggagaagt gtgccacagc 43980
gtcactggtg taccaccttg atttcttgca gatgaacgat gccataacat tgctactgag 44040
aaaaacaaat aaaaatcatg aagacatgga atcaaataaa aatatgcaaa agatggttga 44100
acgaattgta aataaatgtg gtcgtctacc attagcaata cttacaatag gagctgtgct 44160
tgcaactaaa caggtgtcag aatgggagaa attctatgaa caccttcctt cagaactaga 44220
aataaaccca agcctggaag ctttgaggag aatggtgacc ctaggttaca accacctacc 44280
atcccatctg aaaccatgtt ttttgtatct aagtatcttt cctgaggatt ttgaaatcaa 44340
aaggaatcgt ctagtaggta gatggatagc agaagggttt gttagaccaa aggttgggat 44400
gacgactaag gatgtcggag aaagttactt taatgagcta atcaaccgaa gtatgattca 44460
acgatcaaga gtgggcatag caggaaaaat taagacttgt cgaattcatg atatcatccg 44520
tgatatcaca gtttcaatct cgagacagga aaattttgta ttattaccaa tgggagatgg 44580
ctctgattta gttcaggaaa acactcgcca catagcattc catgggagta tgtcctgcaa 44640
aacaggattg gattggagca ttattcgatc attagctatt tttggtgaca gacccaagag 44700
tctagcacat gcagtttgtc cagatcaatt gaggatgtta cgggtcctgg atcttgaaga 44760
tgtgacattc ttaatcactc aaaaagattt cgaccgtatt gcattgttgt gccacttgaa 44820
atacttgagt attggatatt cgtcatccat atattcactt cccagatcca ttggtaaact 44880
acagggccta cagactttga acatgtcaag cacatacatt gcagcactac caagtgagat 44940
cagtaaactc caatgtctgc atactcttcg ttgtagaaga gagcttgatt ttgacaaatt 45000
tagtctaaac cacccaatga agtgcataac taacacaata tgcctgccta aagtattcac 45060
acctttagtt agtcgcgatg atcgtgcaat acaaattgct gaattgcaca tggccaccaa 45120
aagttgctgg tctgaatcat tcggtgtgaa ggtacccaaa ggaataggta agttgcgaga 45180
cttacaggtt ctagagtatg tagatatcag gcggaccagt agtagagcaa tcaaagagct 45240
ggggcagtta agcaagttga ggaaattagg tgtgataaca aaaggctcga caaaggaaaa 45300
atgtaagata ctttatgcag ccattgagaa gctctcttcc ctccaatatc tctatgtgaa 45360
tgctgcgttt ttatcagata ttgaaacact tgagtgccta gattctattt catctcctcc 45420
tcccctactg aggacactca agttgaatgg aagtcttgaa gagatgccta actggattga 45480
gcagctcact cacctgaaga agatctactt attgaggagc aaactaaagg aaggtaaaac 45540
catgctgata cttggggcat tgcccaacct catgggcctt gatctttatt ggaaagctta 45600
ccttggggag aagctagtat tcaaaacagg agcattccca aatcttagaa cactttcgat 45660
ttacgatttg gatcagctaa gagagattag atttgaggac ggcagctcgc cccagttgga 45720
aaagatagaa ataggcgagt gcaggttgga atcagggatt attggtatta tccaccttcc 45780
aaggctcaag gagatttcac ttgaatacga aagtaaagtg gctggacttg ctcagctgga 45840
gggagaagtg cgcacacacc caaatcaccc cgtgctgcga atgagggagg accgaagtga 45900
tcacgacctt gcttgtgacg ccgaaggatc ccctgttgaa gtggaagcaa cggatcccct 45960
cccagagcag gagggagaga gctcgcaggt gatcacgttg acgtcgaaca acaggtcagt 46020
cactccctac atggcagctt aattaacttg tttcaaatta tctttttgtt cagtattagc 46080
gatcgggtga tatcgatgat ttcaactcac cctttcatct ctctggtttt cttaacctaa 46140
cagcgaagag ataagcacag ctcaagctgg ttttatcaag tgatgatctc ctcctccatt 46200
ggcatctccg gtcgtccctg cttctgcggc tgcgcacacc tcgctgttcc gaggaggggt 46260
gctgatctaa ggaggcttcc actttcttca attgcgtctc acgctctcga ttcttccctc 46320
tcgggtatga attgtttcaa tctgaccttt tctcgtgata tgctactggt tccagcatga 46380
gcatttgaac cagcagctta gaattatcgt ttgatcaggt gtttttttta tccattctta 46440
cctgggaact cctcttttcc aactcattca gaacagaaac catgtttatt acactataga 46500
ggggaacacg agttgtggtt ttgttatttc ctttttggtg tgcacaccag gtgtttgcta 46560
gaatgtctga aagagcttgt gtatacttgg ttggctcaac attatgtgtt aggtcatatt 46620
ttatatagtt gctgacctat cacatattgc aggagtaaaa tttttgctat gtttattgtt 46680
ccttgctgtt gattttcctt ttcgtaaaga atattagctt tttagataac gaaaggaata 46740
ttatgtatcc tgcaaaaagg tttgatttgg tggtcactgg ctagtagaaa agcaacgaga 46800
gatcagacaa aacaaagtta tggctacaat agctcattgc aatttatttg ctgaacatgt 46860
gaaaaattaa taattgtcgc atgtcatgtt gctttggcat tctggctgca ggtggttagt 46920
tgtttcatat ccattgtctt ggtctacctt ttcctactaa actagctagg agtagaaaaa 46980
gtaattgttg gttccaagtt caaaagatag gcagatgtta gctaaggaac ttgcagttct 47040
tgatctcctt cagagcttct cacctcacat atatggatct ctgtttatcc taaagcaaca 47100
tttttttaat ctgctagttt caaattgatc atctttgtta ttcttcattt attttatttt 47160
tctccctaag agagtttgct agggtggaga tgtggacaat cgaatgcagc tatgctgttc 47220
aaaccactgt caaaagatgc aggacaggat gggcagatgc ttgttcagtg ttcttgttca 47280
aatgaaacct atactattct ggaagaggtt ggttaattta ctttgtacat aacccttacc 47340
ttgttaatta tcctcttgca aaatgcatgg attggtgaaa tcatgttttt tgtcacgcag 47400
aattgttttc tgaagctcaa agttgaagct gcaatcttca ctgtttctgg cagaaccaaa 47460
ttggtggctt ggtgctgcat acctgcagag aacgcaacgg ccactgcttg tagccttgag 47520
gatgctacat tattgatcat cttactaagg cccccgatca aactttgaat gtaattagat 47580
ctgcccaggt tctcaaaatt tcttggctcc gccgctgctt gaatcgatat gtcatatgct 47640
gcaaattaag tgaattggag atgtacatgc gctcgctata ttgtaattgt ggggtagcaa 47700
ctttatctcg ggtacaacca tggaacaagg gtgatgaatc aaactatttg ctgtcatttg 47760
aagagctcca gcagacttat ttcatgaaat tgtactaatt cttatttgtg tacaattgat 47820
tatctcatta catcttgcat tctgttagac atatgtattt ccatacattg tttacacctg 47880
ctatgactga ttgaggatat ggcaaagctt aaaagatgat gttaacatgg tcacatggag 47940
atgaaggaac atgcatatac agggcactcc attttctgtt ttctctcaat cttatgtaga 48000
gttaatatat ggacagtagc agtagttaca tctttctgtc aactaggcat actacccacg 48060
tgttgctcct ggtcttttcg gcatggtcta tattaagatg taaacttatt tattaaatca 48120
attcaatcgt gtcgcattgg ttcatttatt tacacttgtt atgatatgat ccctgttgta 48180
aaatataaga acttttacga ttctaacact atttataata tttatatgag caattttaca 48240
gtacttaagg aggtaccatg aggtttaaaa atttagtacc tcctagtacc taggtatcaa 48300
gaggtaccaa atataaaata gaaaaagtgg tagctcatca gatactataa aattgctcag 48360
attacaatag tacttgtctc actagttatt ttctattcag ttcttttgtc ttacccccac 48420
atacctttcc acactcatcc atttctctgg tgaataacta aaaatgttta tatttcagag 48480
cggaggtagc aagaaaatac aaataactag agtaaaggct gttgaatagt ctcacattag 48540
ttgtgaaagg gcaaatggca caatatataa gtggggtagc cccacacctt attacctagc 48600
ttttgtgtgg gaaaagacct tttacgatct tacaattggt atcagaggct ggctaattca 48660
acatctttga cccaagggca ctactagaaa aagggttttc actgtcggcc agaggtgctg 48720
ccagcaacaa aaggccagag aaaataatac actttcacta atggtcgatc gacctccagc 48780
gaaaatcaat tttcgttggc ggaggctcat tttcactggc agtggggcgg ccaccagcaa 48840
aaagttggtt tcactggtgt tggtatttct tacgaccaca gataaatccg caagcgcacg 48900
gaataccgca gtagcacttc acattcgggt gaccccaaaa ggatatcgaa ctcagggtac 48960
gtgtataatc tacctaagac taagacaatc taaggacaac aactgatggt aggcaggcta 49020
gactagagag gactttctat gtgttaatta tctacgctaa gcaaaggtaa tttcctatct 49080
gttgcttcag gcaccaaccc ctgctgatct gccagcgtgg ttccggctat agctctatga 49140
tgtatccgaa cgttgaggat tactaagacg ggattcaggg ctgtcaccac ctgctatcta 49200
cctctgacaa atccgtggga tacagaacaa acaaaggtaa tctctaacct agacaccacg 49260
tctaagttat taattactac tctaatactt gcgtaggaac ttcctttaat atccagagtc 49320
ttagtactag agcacttagt atgaatacta aacaataaac ccgcaaagat agaaatctat 49380
cttacttaga ctaagtaatt aaataacata agaaaggaac acgaatgctt actttataga 49440
agaagttctc caaacccgga gtagagtgta ccgacagctc cggtactcct ccagaattcc 49500
tcctagaagg ctacactcgt caagaaagaa gttggagaga gcgccaaact tccgggcaca 49560
cctccagagc ttcccctcac tctctacctt attctaatgt acacaagata gaatagagcc 49620
tcttgcaatt cagctcaaag gtgtgtgttg tgaatgagag tgaggagcac tatttatagc 49680
ctccatatga cggttacagg tatgggaaat gaccaaacta ccctctaacc atcattggga 49740
tgaaatcagg accgtccagg tgaaatatgg cgatcaacgg cctccagatg ggttcggccg 49800
aacccaggac ggctccgttc aacccggttt tcctcatgga cactaatttt gaggccctca 49860
gcccattggc atggtggatt gggccatttg gaggtgttta attgacttat ggggcctcca 49920
atccgtgtaa acgtgtcctg aatgagcgga ggtgcatttt atcgcatgac ggatgtgttt 49980
tctctttaaa atatctgcat acacatattt caccaacgct agtggaaatg atactatgtt 50040
ttatttcatt atatgcaggt atggacggtc aaatgttatg atttaaggac cgtcaacagc 50100
tggcaacaag taatgagacc cgccagtgaa aataattttg gagaaaacaa atataaaaat 50160
cagatgtggg aactgtcgac ggcatgatca gttggtgagg gtcaacagcg tagtgttgtt 50220
ggtgtcgccg ccaccctctc ccccaccatc cggtagtttc cgctgtcatc cttcaccacc 50280
gtgttgtcat tgttattctc attcttgctg ttgtccctat agtggccctc atcgggggac 50340
gtgagcaacg aggtggattg tgagagggag gtgagcgtga acgcttcgag cagcgaggcg 50400
gaccacgaga gggcagggac agcatggcca ttgccagatc cactgccacc acccctcatc 50460
ttcgacacca tgtaagcgga cccggtggtc ctaagcgttg ccgccgccgc cggatctacc 50520
acctccaccg ccaccacgcc tctccttcgc caccaccaga tccatcccct ccactatcac 50580
cgcttgtctc cttcgacgct gtgaaggaag atctgccaag gggaagtcac tgttgtcgtt 50640
gttttctgct tgcggccgat ggcgaggtcg aggtcactgc taccgacacc gaaccgcaac 50700
cctagctgct ggatccatcc actacaccgt cgccgcccgt ctccttcccc gccaccggat 50760
gctcgcatcg cgacccaccg ctgctggatc cgagccctcg tgacccgctg ttgccggatc 50820
catgccccct cccctctggc caaatctagg agagggaggg gagggttgtg tcgccgtcgc 50880
cactccctgt agtgcggcca cctagtcgtc gaggccgtgc gcgttctgca ctggaccacg 50940
ccgctgcctc tcctgcgcgc tgccgccgag ctctgccatg caatgccacc tggccaagcc 51000
gtgtcgctgc tgagatagcc gagaaatcga gagagagaga gagagagaga gagagagaga 51060
gagaggagat agatagattg agagagaatt tgtgccgctg ctgagatacc gagagagaga 51120
gaagatatcg ctgagaggga accgagaaaa aaaaagagga agtgaaaatt tgacttccgc 51180
gcatgctccc acgatttttg ctggcggtgg cttaggtggg cagctagtga aaattgattt 51240
ttgctggcag tggcttaggt gggcctctag cgaaaagggt gcatcttcgc tggccggccg 51300
ccagcaaaaa tacctgttgg taccggattc caaaaatttg tgcaccgtta cccacaagac 51360
aagatccttt atttctaacc atttcttaac ccggccgcca gcaaagatcc attttcgctg 51420
gcagtccttt gccccacacc cctcgttaca ttttctctgg cggtgttcgt gtatggtcgc 51480
cagagaaaaa agataggtgg acgccatgaa aaattgattt tgtagtagta gagacagtgt 51540
gtgaaagcct gatggaccgt gggtgcctgt gcggcctgtc tacgtgaggg actgtggggt 51600
atggcaaagg ggcggtaaac ggctgaggga ctgttgacgg ctgttaagta caaaattctt 51660
gtcgtcaata cctgcataaa accataaaag taaaacaacc aacatagtag tatgatttat 51720
ttctaactat tttcacgagt gttggtgtat attcgtatgc aagtaatcaa ccaccaaagt 51780
tgggagagaa tgatcgtgaa gaatgcaaat atactttggg atgatggacc cacatggttt 51840
tgaactataa catataaaat gtgcaatcat tttgagctaa aacatggtgt gccataaagt 51900
ggggcccaca tgtcagcatc ctgaggaagg actaggacag cagagccggt cagggtatct 51960
agcccaattg tatgatggtt gtactcctca tcccaatgac ggttgtggcc gtttctaccc 52020
gtggaaaccg tcataaagag gacacttgtc aagaggagag tgaagattcc atttagatgc 52080
ttagcatctt cacattccca aggcatcttt ccacactata aataaccctc tcctctccct 52140
tattcacaca caccaaagca tgagctgaat tacaagatta gggagagagt aaggtagaag 52200
aaatcggacg aattccggag ttgtcggtaa tctcctctta cttttgtatc ttgttaatta 52260
ctctgcttta atagaaatat cattctgagt aattaagatt ttcttaatga gaattacttc 52320
ttggttagtt cctaattagc atacatgatc attgttcact gtagtctatt aaattatagt 52380
gattgattta gtgtttgatc ggcactagag tagttattaa ttgcgtagac gcggtgtctg 52440
tgtaattaat ttcctgttgt tgttgtgtat cccatagtac gtttgaggtg ggtgtagagg 52500
tgctgatagc cctcaagagc acttaagtcc tccctgtcta ggtacatagt agagcgatat 52560
ccgggaacag cgggttgcca gtacctgaag tattgcatta gattaaaatt aaactttccc 52620
tagacattgt ttgtcattag gatgtcctct gtcttctgcc cacattagct ttgtgtcctt 52680
ggatgaacct gacgaggagt taaactcaca cgttcaatgc ggaaatcgat acactggaat 52740
actcccgggt gaagtgctac atcagtatct tccgtgtgct tgccaattct atctgtgacg 52800
ttaagaaata ccaataggga caacaaggtg tgaaggggga aattattgaa tagtcccaca 52860
ttggttatta aatggcaaat gacctaatat ataaatgggg gagcctctca ccttattggc 52920
tagcttttgg agtgggaaaa ccctttaaga tcttacaaag gcatgcaata tttatgtggc 52980
ataaaaaaca attttctttt cctttttatc atttttatta taacatgtgg ctgatttgat 53040
acatgatatg aaagtttggc cattaattgt tctatatagt taatgctagt acctactccc 53100
tccgttgcag aatacaaaca tttttggttg gatatgtgta ttaagaaatt atgtagattt 53160
aaattaggaa tattatgatt agttgaacag agaaggtatg tgagaaaatt gaatggtaga 53220
ggattgtgat tggtcggaaa gataatattg gtggagaagt tgttatattt taggacaaat 53280
cttaaatact agaaattgtt atattttggg acgaagggag taaaatatac cgacctacca 53340
ctacgaatct gaatggaagg ttactagagt ttattcagat tgattgtact aggttgtatc 53400
tattctatta ctaggttgct atattttagg atagagagag tatcaaatga cttgttgaac 53460
taatagtggt gttaagagcc tctttgggga gcttctagca attataattt ctcccataat 53520
atatagattt ccatacagtg tagattctta cctacattct aagaagctag gaaacctagg 53580
ttttcagaca cttagaagct ggctaccaag tggtgcttct caggatctga agctcccgaa 53640
acaatagctt tctcgaccca atggcttcta acagtatgtg tggcggtggc aatttcccaa 53700
aaccccatga cctctcgcgc atggaaatca gtcacattga cgagcaagct catctcttta 53760
agtctcagtt cgtttgggct cattatctta aagaaccatt ccaacttaac tgggctcctt 53820
aatcttaatg aaataaaagc ttagcttggc ttgactcgcg agctgatttg ttaagctttt 53880
gttaggcttg ttaacatatt tatatatcct ttataatttt aaatttacca ttttatcata 53940
ttaacatagt agtaacacta aatataaata gcttggataa aattgaaaga ctaaataata 54000
caaagtatat gtagttatga tgatctcttg tactaacaag ctaaatgagc aatctgtgag 54060
ctgcctcatt aagctcacaa gctaaaagcc tatctccgct tgactcataa tgattacaag 54120
taatctaaaa aaaataatta caagttcgaa caaaactcac tacaaaccaa tccaaaccac 54180
aatttttgag ctttctttcc gtccctagca gtgacgcttg tgttgagacg aatcaacatg 54240
acactatagg tcacacccct tcctggtacc acctaatagt gacaccagag ggagtgcgcc 54300
gtaggagatc agtgtgaacc tactcccacg gaaccgctat gctggtacca gaaagctagg 54360
cggcagccgg cgggcatggt ggcgctgcgg aaaacgcgga ggcgagcgaa tagcgatgac 54420
gtcgccccct ggtgtgtcac gcaagttacc gcatcaagca cctgcgcgta gaatctcatt 54480
agtgaatcgg actttctcgt tagcggaaag aatcaaattg ctccctacgg gtgcacatga 54540
tttaacgtgg gtgtgaaatg ggaaagggcg ttgcaaaagt ccgtgcaata taaggattga 54600
cggatgacaa aaacaatagt tatgttttaa gtaggtaaag ataaatatag acagatgctc 54660
cggggccatt actgccggta aacccaagcc aaaattggaa atttttctac caaaagatct 54720
tattagtgtc ggttctaata acgaacctgc actgctgata agtgccttat cagtgccctt 54780
ttttttttac aaaccaacac tgataagacc cgtcatatta aaaccatttt atttgttgaa 54840
ttggcactga tactgccagt tcttgctcct aggagcgcgc acgggctcaa gtgaacgagt 54900
gacctctacc agtaccagtt tttgtctaac tcgcacttaa ggatcgtgtt caatcccgag 54960
atcttttaat tgtttacacc tagaggcatc cgccaccttt tggtcgtcgc cgccacctcc 55020
attgtccttg ttataaaatc ggacccggta gcacatcgag aaataaacac agaagaaacg 55080
tcataggaga tccaaacacg atatcgccgc tgaaccaaga tattagtaga ggtcctgaac 55140
cggctttcct ccgtctgaat cggcagtcgt agccactgaa tcagctttct gttcctgaaa 55200
accatcaacg aaggaggggc agatcgatcc cctttctccc atcctcgctc ctcgcgctca 55260
cacggcactc ctcagcaccc gccactgctg ccgccaacac aggagccgcc gcgcatcact 55320
gctcctctgt gcccctgagg acgtcaccgc cggtggccac ttccgccgcc gcctccttgc 55380
ctcctgaacg ctgccggtga gagtgccgcc gctgtgggct tcccgccggt cgaggacgcc 55440
gatgcccacg agtcgctcca acgcagagga agtcgccgcc ctgctgtcgg aacccgccgc 55500
tccaccacct ggagccggcg ccgccaagtc gtccgccgcc tgcaccgctg tttgatccgc 55560
tcgacgccaa aaccatagtc gccagccgtt aataattcgg caaatttagg aggaagtttg 55620
aggctctcgt gtcgctctca cgcgggtggc tcgggggcag aaccgccgct ggaaaccgcg 55680
cggccggccc ggaaggtagc ggcggggaaa atgtgccggc ggtggagttt tcttctcggc 55740
gtgggaggag aaacagcgcg cgtacggcgg gaggcggcgg ccggcggggc gcatctgcct 55800
ccggcaacat tcgccatgca tcttcatttg tctccgcttg atgctgaccg aaggggtccc 55860
tgacctacca ccaacacaaa tgattgtttt gtattcgtgc catgcatgac ccggtccagt 55920
actgcctgca cgcggagagt gcaacgaacc tcactaccca cctaatagga ctagtaaacg 55980
agaagtcaag gtattttatt gttgctcatg gctttgtgga ccgaattggg atattggatt 56040
ggtcaggtac ctgagtgagt actacgctgt taatatagat tgacatgaaa tggaaaacag 56100
agtcctcggc gtctaaaccc acctaaccca tgatagactg ctggtaattt aatttcaaat 56160
gtcaggggaa tatgattgtc tgcatgatgc gcgagtgata taaactccat gattaagctt 56220
cctcactgca aaagctacca tttctgattc aatcgacaga cagaagagct ggtcgccaag 56280
ttctctcccc tgtgcagtct ttggcgaagg tgaagaatcc attcaatcga tggcggagac 56340
ggtgctgagc atggcgaggt cgctggtggg cagtgccatc agcaaggcca cctctgcggc 56400
ggcccatgag gcgagcctcc tactcggcgt ccagaaggac atctggtgcg tactgcacga 56460
aaacctcacc cgtagtttcc atcttcgctt ccgttttatt gattgccagt ttcaaccaaa 56520
atatgtcagg caaaactatt gctgaaatgc gcaaatatat cagggactta atatatgttt 56580
ttactttgcc tatgggtgcg ttcctttttc ttgaaacggt tgcaccccag cttttagtgg 56640
aagcatatct ctatctctac taatataaaa attaaagata tttttaccgg tactttgata 56700
cgtcatccat gtatgagtct gttttaagtt cgttcgattt tggaaataca tatccatata 56760
tccatatttg aatcggtttt taagttcgtt cccttttgga aatatagaag gagtcgaata 56820
agaaatctct ttgaaaaaac tcgcatgcta acctgagatg atcggactcc taattgcaac 56880
tcatgatttt ctaaaaatat atatatccaa gcaaattacc acaacgaatt tcaccttaat 56940
taaaccgtat aacaataata agaaataacc ttcatctgtt gtaacgcatg ggcatttttt 57000
ctagtaaatt ataaagttat cttagtagtg aatgggacac caataatctt tctaaaacat 57060
ctctttaagt ggaggacgca aatctttgcc tttaattttg aaaaatattt cttcttgtca 57120
attaagcaaa gaaagtccct cggtggtcga aattaaccgt tccataaatc atgtgtgcta 57180
aatccactga actgaagtag aactgtttaa aatacgagaa attttttatg ccttgagaga 57240
taccaagacg tacctgttgt cgaaagacaa ctgtttcata tcgaaagacg tggttcactc 57300
agtgaattaa tggaatagta aaagcaatat attaattggt aagaaatttt ttgggatctc 57360
ccattatatg atcttagggg gctatttata gccttaacac aaactcatgc ctcctagggt 57420
ttaatttgta taggggaatt tacatgggta cccttatgat agggacttta gtgagggcac 57480
acaatatcat ttgtgtttat gggctcttac gtgaactcct tgggccggcc cattactggt 57540
gaaaggcctc catggtgtct cccaagccct ctgccgaagc cacttctaac aagagcgaaa 57600
ccctcctgct tcgctctacg gcctttgccc gaagtgatgg ctcgttaggg tgatgggctt 57660
ggtggcgaac ccctcggtct tcatcatcta acttcgtcag ctgcttcgcc ttatggcttc 57720
gcctcatgga ctcagctaac ctcaaggttc tcgtggtttc agcaggcccg gccgaaggcc 57780
ctcgtgatat gcctccaaca gtaccataaa ttttcatacc tcctagtacc gtagatacca 57840
aattttatat tagaaaatat attcggtacc tcctcaaaga ttgaaaaatc actcttaaac 57900
tacacacatg tccataaact tggaccaatc aatcatgtca gtgcgtgaac aagtaagata 57960
tgtaccgata tcctaacact actgggaacc catctttagt cccagtttgc aaccctcttt 58020
agtcccggtt acaaaccgag acgcccaaag cacatcttta gtcccgattg gtatttgatt 58080
tcgagcggtg ccatgcccca catcgagttg ctctcgacat ccccaaatcc ccacatcaaa 58140
ttgacatcat atatcgaatg aacatattca acaacaaaat catccataaa ttcacaaaaa 58200
catcttaaaa aatctacaac aaaatcattc atccacaaat tcttttcttt attcacttct 58260
ctccttgctt ctcctcgggc aaggcggctg gcaagcccgg tcgagctcct cgtcgccgcc 58320
tcgagggagg gccaacagcc acgtgaggag ataaggacgc ggctctctcc ctccaccgtg 58380
cgcctctttt taatttttag tcccggctgc tataaacaat ggggactaaa gtgatgatct 58440
ttaccaatcg ggactaaaga tcgttggccc ctataacaat tgaaacgtgg ctaaaaataa 58500
tctttagtca tggttggtgt taccaaccgg gaatataaga ttataaagtg tcgttgggct 58560
tttcaaccgg ggctaaaggt attttatagt cccggtttct atttgaaccg ggacaaatgt 58620
gatttttgtc atccgggcaa agatcagttc tccattagtg taatctgggc aaattttatt 58680
acatgacacc tgaaaattgt gtaatttgct catagacatc gaaaaatgtg tcatggatga 58740
atgatattta aaagattgat aatttgcaag agaacacttt caactcaaat tggagggata 58800
aattgttaag aaagacatga atgcccctat gactacatgc ttcaaacatg acgtcagagt 58860
aaaaattttg aaattatgca actctaaaat taccatcatc ccaagtacat cactacatac 58920
attatattca tgttcactcc aacctctgtt caaaagcttt tgaccctagg ggtattctcg 58980
tatttttgaa gaatttttct cttcaaataa gctgaaagcg tcctccagca aattatcagt 59040
ttttaaagtg tcttttagcc gtgacatgtt tttacgatta ctaccagcaa actacacaaa 59100
ttttgattgt cctgtagcaa aaatttgctc ctaatctttg cataatgtag cattagagag 59160
gtctaaagtg catatgacat ggttcgactg ttgatgtggc caagaatttt gcctttagac 59220
cctcgtgcta tagtacgcat tcaagtagac tctcaccact cccaaatatt tttgtacatc 59280
acttaaaata caaaatttct aaatgttgag aaatgcatat ttgcttattt cgggtggtaa 59340
gaatcgattt tagaggcaaa cagttgtgaa gggacctaag tgtaaaattc tcgccaccgt 59400
agccaggcat gtcagcattt gagttactag ctgactgccc atgcatgctt tgcataggaa 59460
ttaaaaaaaa aactatcgtg cgctctaaag ccccccaaaa acataataaa aatatcccat 59520
tgtattcaat ttcacattat tttttgtaca tgtatgtgat gctatggaat tggtcccaaa 59580
tgtcagtggt ggtgggtagt gacagattca ccattcatca ccattgcagt cttttaaata 59640
accatctaca gtttagactt gaaatgatac attaaaccaa tattaaatac ccatatgtgc 59700
ttataagttt atgtacctaa atgaaccttc ggtttcaaat tcaaggacgg gtcatgtatt 59760
ttactcaaaa acttatgatg tctttaaagt atatattgta tgaaaattta gtcattttat 59820
tatttttcta atataagatt cggatttcta ctagagatat cctaaattac tttgaaatga 59880
atgattgcag gtatatcaaa gatgagctga aaacaatgca agcgttccta cgagctgctg 59940
aagttatgaa gaagaaagat gagctcttaa aggtttgggc agaacagata cgtgacttgt 60000
cctatgacat tgaagattgc cttgacgaat tcaaggtcca tattgagagc caaaatctgt 60060
tttatcagat ggtgaagctc agaaagcgcc atctgatagc tacccaaatc cgcaacctca 60120
aatcaagagt tgaagaagtg agtagcagaa actcacgcta caatttagtc aaacctattt 60180
catccagcaa tgaggatgac atggattgtt acgcagaaga cattcgtaat cagtcaacta 60240
gcaatgtgga tgaaactgag cttgtggggt tctctgactc taagataagg ttgctcgaat 60300
taatcagtgc caatgtcaat aatggtccaa ccaaagtaat atgtgtggtt gggatgggtg 60360
gtttaggcaa gacagctctt tccaggaaga tctttgaaag caaagaggac atcggaaaga 60420
actttccatg taatgcatgg atcactgtgt cacaatcatt taacaggatt gagctactca 60480
aagatatgat acggcaattt ctaggttcca attcattgga tcaagtcttg caagaattgc 60540
aggggaaaat ggtggtgcaa atacctcatc tttctgacta cttgagaaaa aagctcaagg 60600
aaaagaggta ttttgttgtt cttgatgatc tatggtcttt agatgcatgg aactggatta 60660
atgatattgc atttcctaag aataacaata agggcagtcg gattgtagta accacacgag 60720
atgttggtct agccgagaag tgtaccacaa cttccctagt ctaccatctt gaacacttgc 60780
agatgaatga tgccataact ttgctactga gaaaaacaaa tagaacacat gaggacatgg 60840
gaacaaacaa aaatatgcaa aaaatagttg aacaaattgt aaataagtgt ggccgtctac 60900
cattagcgat acttacaata ggagctgtgt tggcaactaa acaagtttta gaatgggaga 60960
aattttataa acagcttcct tcagaacttg aaagcaaccc aagccttcaa gctttgagga 61020
gaatggtgac ccttggctac aaccacctgc catctcatct gaaatcatgc tttttgtacc 61080
taagtatctt ccctgaggat tttgagatca aaaggagtcg cttggtagac agatggattg 61140
cagaggggtt tgttagagcc aaggttggga tgacgaccaa ggatgttggg gatagttact 61200
ttaatgagct aatcaaccga agtatgattc aaagatcaag agtgggcata gaaggaaaaa 61260
ttaagagttg ccgagtccat gatatcatgc gtgatatcac agtttcaatc tctagagagg 61320
agaactttgt attcttacca gtgcatgatg gctccaatct agcacaggaa aacactcgcc 61380
acatagcatt gcatgggagc atgtcctgca aaacaggatt ggattggagc attattcgat 61440
ccttagctat ttttggtgac agacccaata atctagcaca cactatttgt tcaaataaat 61500
tcaggatgtt acgggtgttg gatcttgaag atgtgaagtt tttaatcaca caaaaagatt 61560
tcaacaatat agcattgttg cgtcacttga agtacttgag ctttggaaga atattctcgt 61620
catgcatata tactcttccc agatctattg gtaaattaca tggcttacag accttgaata 61680
tgtcaagtac atacattgca acactaccaa ctgagatcag taaactccaa tgtctgcgca 61740
ctcttcgttg tacaagggta tctaataata acaattttag tataaaacac ccggtgaagt 61800
gcttaactaa cacaatgtgt ctgcctaata tattcacacc ttcagttagt agcgacaatc 61860
gtgccaaaca aattgctgaa ttgcacatgg ccaccaaaag ttgttggtct gaatcataca 61920
gtgtgaaggt acccaaaggt ataggcaagt tgggagaatt acagatacta gagcatgtgg 61980
atatcaggag gactaatact agtgcaatcc aagaattggc gcaactaagc aagctgacta 62040
aattaagtgt gacaacaaag ggatccacag aggaaaaatg taagatactc tatagagcca 62100
tccaaaggct ctgttccctg caatctctcc gtgtggatgc tgaggggtcc tcaggaaatg 62160
gaacacttaa atgtctagat tctatttcct atcctcctct gttactaaag acactcaagt 62220
tgtatggaga tcttgaagag atgcccaact ggattgagca gctctcgcac ctcatgaagt 62280
tctacttatt agggagtaaa ctaaaggaag gaaaaaccat gctgatactt ggggcattgc 62340
ccaacctcat gctgctttgt ctttcacttg atgcttacct cggggagaat ctagtattca 62400
gaacaggagc attccaaaag ctcaggacac tttggtttga caagctggat cagctaagag 62460
agattaggtt tgagaacgac agctcgcccc tattggaaaa gataggaatc ccatactgca 62520
ggttggaaat agggattatt ggtatctcta accttatgag gctaaaggaa attacacttg 62580
gatacagagt taaagttggt tatcttggtc agttggaaag agaagttggc acacacccaa 62640
atcgccccgt gctacgtatg gaggaggacc gaagctgtca cgacctgaga agggatggca 62700
aaggatcagc tgtagaaatg gacgcaacgg agcccctccc tgagcccgag agctagaact 62760
cacaggtgag cacgttgaca agccaattcc ctgcattgca gctctactag atttctcttc 62820
tgttcagtat tagccatcag ctgtgatgat tccaaccaac ctttcatctc tcctgtttta 62880
ttaacataac aatgatccaa taatggaatg ctttctaaaa ggaaatctat caccggccaa 62940
tgatgtggat aatcctagaa agacttcgag agaagagatg aaaatgttac aaatgttaat 63000
caccactgat acttcgttga tgtcaacaaa agaggtaaat ttgagtgagc tgccttacga 63060
tctatccgag aggcatcctg cttttccttt ggtttaccaa ctcctgaaac tagtattggt 63120
tttgcccgtt gcaaccgcat cagtggagcg atgcttttca gcaatgaaaa tcatgaagac 63180
aatatactac agaacagata agtgataaac ttatgaatga ttgtattgta tgcttcatga 63240
gccatgtttt ctagctacaa ttcgaaatga tgacatgaaa gctctccttg atcaaaagga 63300
gggaacttgc aaagagaaga tgtaataggt aattaactat tccaacagag gtgttgtata 63360
tttatatcat acagtgctct taatttattt agcctattat aatgcatgat attagtctat 63420
taaagcaacc agtttatact tattctaccg gttttgttag catatattta atattcgtag 63480
tgactagtga cctgacccca gcgaccatta attttttcct tcttccgccc tggagaagag 63540
ctaggcacag ctgtagctgg ctgatggtct cctcctcctc tccagcgcca tcctctcctt 63600
gcacactggc tctccgtctc cgggcgtccc tcctgcgtgc acctcaccgc tccgaggaca 63660
tgatgatcaa cgaggcttcc tgcttccagt tgctaaatat aatatgtgcc taacactctc 63720
agttgtttcc aacccagggt atgaaattga tctatcagat tcgctccttg ccacaaggtg 63780
caaacatttg atgcttagtt tgatcagggt gttctgtttc agggttgtgg tttgcacaat 63840
tactcccact ttgtgcttag atatgcttag gctgagtttg aggagaaggg gacagagaag 63900
attgggaagt tacgcaaaat gaggtgagct attacactag tacagaacct ttcatctgtg 63960
aacatgcttg gaattagcgg ccgtcacaag aaattcatct caatcgggtc gacaaagcgc 64020
cggattgaga taactcaggc ccgtcacgga tcacacatat cagtgacggg ccgtagttta 64080
gacccgattg agataacatt ccatatctcg attcacgagt gcagaatgag taattcgtac 64140
ttctgtgcat tgtatttatt tccctacatc tttttgctgt gttggaaata agagtgtttt 64200
ttgcacgtac ggtacactgg tgctccaata atctgtgttt tgtctaattc ttagggagat 64260
aaacatgaaa tactatagca gcgtacttat gcaacaccgc tacgttagtg cactggtgca 64320
gccttttctg ttgttatgta attatcttct gagatgttct atatatatat ttcaagtttc 64380
tctctattgc aattttgtga tcgcagtttc ttcttttcag ccggatatga agattgctgt 64440
gatgctgctg ctatagcttg tactcactga ttcctcactc tttctacttc atctttcttg 64500
ttcagttttt attttatttt tttagtgtgt gaccacacat ttgaattgaa tatggtctgt 64560
gtattgtaag cgtacagtta attgggagtg tgcatctatt cttaagtgtg ctcggcactt 64620
ttgaacttag atgggaatta ttggaatgtg caactatatt cgtttacaac acttacatat 64680
actattcgta ctcagtcgtg tgctgctatt taaacttgaa gaagttcttt tttttggata 64740
acaggtatac aacataattg aagtatggtg caacacaata aaaccattaa cgtaagtcca 64800
taagcaaaca atttaaaagt tatgggtgat atgtattttg aaagagatga aatgtattag 64860
cctatataag tcatcttcaa attgcaacga tccatgttgc aatagatttg caggcttgct 64920
gccttccggg aaccggacaa aactaacatg tgggggtgag ctggagcttg actttgatgt 64980
tgttgtcaaa gaccggaatg gtttggtcat cccatcttta ctcgtgttaa ttagtttagt 65040
aagtattatg ttgtagcgtg ggcatcttat tatccatctc aatgagtatt acttaatttt 65100
atatttatta gttacctcac aggggctgta ctcatcttaa gtcttacatc ttccgtttca 65160
caatgtaagt cattctagca tttcccatat ttatattgat gttaatgaat ttagacatga 65220
atctagatag atatatatgt ctagattcat taatatcaat atgaatgaag aaaatgctag 65280
aatgacttat attgtgaaac ggaaaaaata ccatccattt caacccccaa tttcaggctt 65340
agtttgattt aaattatcga gcggattgtt gtgacagatt tcctgcaata gggaaaaatt 65400
aaatattttg agaaatcagc ttaataaata gcttaaaata atcttagaaa tgcagtacaa 65460
gaattacttt tttaataacg tatatttcca tgtgtggaga aaataatcca ctaaataatt 65520
aatctgaaaa aactgagccc aacgacctgt tcatatgcct ttgtccgggg taaatatatg 65580
atgggaacaa agagtataga agttccttaa aaaatttgga aacaaattaa actcacatat 65640
agataaaggt gttatattgg acgaacctgc aaagcctaaa gataagctca aatttgtctg 65700
tggaaaaact acaaattgga cgcccaattt ttttctaaaa gatcgagcgt tgtttcacca 65760
ccttatctaa aaaaatgttt cagtgcctaa caaaatgatg tttcggctac agaaatgatg 65820
aaacacaatt aaatcactct tggaacattt ttctacaaca tataaaacat cggtttcaaa 65880
ggtgttgaac aacagcaaat ctgaattcat cgaagaactc actgcaacat tttaattcaa 65940
taaattaaac atttagatac aaagctaaaa catgacgaat ccaaaaaata taatgaaaca 66000
ccaccgagaa ctgacagcaa catcttgatt aatctaatga aacatttaga tacacatgct 66060
gaaacatcga aaatccaaaa aaaaaaatca ggctagatct acctcttcct ccactttggt 66120
gctatcctcc ggtcttcgtt ggggccgtcg atgttgcgtg agaagaggat gtggccgtca 66180
aggatgtcgc ggcggcgcca ggcattcagg acgacgagga aaaatggtcg gtgaagtcga 66240
tgacgtcgta ggcgatggca tagcatgggt agtgcgcctc catggggatg aggctgctgt 66300
cgacgtcgag gaaaccgaaa agctgcggtg gatgttgacg acgtggatcc ggtggaggag 66360
gtggcacaag tgaggttggc ggaagagagg agtcggcgtg ggcgaaggag cgcgggggag 66420
aggaggtggc gcagacgaga gaggcggggg agaggagatg atgcggaatg aggcgatgcg 66480
aaagaagccc gatacttttg ttcgacgtcg gtcgcccgag gcagaacatt atcgatatga 66540
gttaactaaa attgcaacaa gtctacgaga gcttgtttga ggagcttata gcagcttttt 66600
ctctccgaat ttccagttac ataaatcatg taaattctca ccaataatct atagttatag 66660
tagaatttac agcatcaaaa taatcaagta tatataatct ataagtaaaa ttcatagcta 66720
agaaatctag ctttccgata ttctcgagga ctgactacca acctttttta taatcttaaa 66780
aatcctctag acatgaccta aaattgtatg gaatgtaaaa aaatacggga ttttcacatg 66840
ttcaattccc ctaaaaatgt tgggcgcatg accaaattgc aaaatgtacc aggcccgaga 66900
tcgatcgatc tgaacggacg cacgaaggaa gaaaaagtgt aggagttgcg gcacatcacc 66960
gccgccgctc ctacaccgtc cgccatagcg attcccttcc atctcgcagc catccaaagt 67020
gagtccttct tcgccgtctc cgatctggaa cttcaccgcc gccgccgccg catctcctcc 67080
ttcctctctt ggcaactgtc ggcggcagcc aacggctctc ttcccccctt gttcctcctg 67140
ctatcgcgag tcaacgggat gctcgattcg ttacattttt tttaagtact agtattgcta 67200
atagttgcga cagagaaaaa aaaaagaaag tgtgggacgg aagggaaagg ggggaattcg 67260
ctataaaagc actcggaatt ggcctgctcg atgcgccgag gctgactgat ctcctgcagt 67320
tccaagactg aacagcgaga tctctgcagg tatgtgcccg ttagctgact ccacgagttc 67380
tttttttcct ggttcaatgt ttccactgct ctctctctct cttttaaaat tcggcaagag 67440
ctgaacactt cggctgtgaa tctatgatgt cttcagcaga tctgggtgca gtgatttgat 67500
tcgcaggtgt aagcttgatt agccattact tgaggagtta ggtttcatgc ttggataata 67560
atcaggccta caaagttttg gtattgtcgt actaaggtga ttattgccgc gttccccccc 67620
tccccccccc tttttttttc atttacattt gaactttggc atgtgtattg gatcatgctt 67680
atggttcaaa acaattacgg tactaaagtt tacttgcatt agtattaact tttacagtat 67740
atatgtagtt atgtacaaac tacaaacaat cgcatgcttg ttaacgtaaa taaggttttc 67800
ccagcatcta ggaattttct agctttgtaa ttattttgag acaaattttg aatgataaaa 67860
gttgctatat ttgggacgga aaggtagata tccacgaggt gacttaactc tttccgctgg 67920
cttttggacg tgctacattc tgataaaaat aaccgcatct agtcactctg tacatattcc 67980
aaccttgcaa ggaaatagat actacctccg tttcaggtta taagactttc tagcattgtc 68040
catattcata taaatattaa tgaatttagg catacatata tgtctagatt cattaatata 68100
tatatgaata tgggcaatgc tagaaagtct tttaatatta aacggaggaa gtacaattta 68160
acggaatagg cttcgattct gagatccatt gttctaacag gactgatctg gtggtaatgg 68220
cggagacggt gttgagcatg gcaaggtcgc tggtggggaa tgccatcacg aaggccggag 68280
aggcagctgc tgcagagata agcttactga ttggtgtgaa caaggagatc tggtaggttt 68340
tctttaatta cttacgcaaa caaaatattg gtttaggaaa acgttaaccc taaactttcc 68400
aaactggtta ttttcaaaca cccaccccca ccccccaaaa aaagaaacaa aactcctggt 68460
accaagtggt aaaaccattt taagtgatta ttcgaattga ttttcatact ttaggtgata 68520
gaataaattt aagccatgta atgtcctaaa attgtccttt cattattagg tttatcaaag 68580
acgagctgaa aacgatgcaa gcattcctga tgacagctga agagatggag aaaaaaccca 68640
ggctgttgaa agcgtgggtg gagcaagtaa gggatttatc ttttgacatt gaagattgcc 68700
tcgctgagtt tatggttcac gtggggagca aaagcttgtc acaacagttg atgaagctca 68760
aacatcgcca tcgcattgcc atccagattc gtgacctcaa atcaagagtt gaagaagtga 68820
gcgataggaa ttcacggtac tcattaatca gccctaacac tgatgagcat gacaccttga 68880
gggatgaatt tcgctactgg tcagctaaga acattgatga ggctgaactt gtgggttttg 68940
atgatgcaaa ggaaagtata cttaatttga tcgatgtcca tgctaaccat ggtcttgcta 69000
aagtgatctt tgtagttggc atgggcggtc tagggaagac aagtcttgtt aaaaaggttt 69060
accatagtat taatattgtt aataatttct catgccgtgc ttgggtcact gtgtcacagt 69120
catttgtcag gacagagctc ctgagaggac taatcaagca acttttgggt ggtgattcgg 69180
aaaatgaaca cttcaaaggt cttcagagca tgcaaaggaa tgagaaagtg gaagacctcg 69240
tggaagactt gaagcaaggt ctaaaagaga aaaggtactt tgttgttcta gatgacatgt 69300
ggagcataga tgcattgaat tggcttaatg aatctgtttt tcctgactct aaaaatggag 69360
gaagtcgcat aatagtaacc acgagagatg ccagcataat tcaaaactgt gcttatcctt 69420
cttatctgta ccgccttgaa cccttaaaaa cagatgatgc taaacaattg ctgctgagaa 69480
aatcaaataa aagttatgag gacataaaaa gaggcaaggc tgagaaggtg tttgatagga 69540
tactagaaag atgtggaggc ttaccactag ctcttgttgc gataggagct gtccttcgca 69600
cgaaatgcat agaagattgg gaaaaactgt ctctgcagct atcttcaggg ctcaaaacaa 69660
agtcaagtct tgaagaaatg actagagtaa ttacacttag ttatacacac ttgccatctc 69720
atctcaagcc atgttttctg taccttagca ttttcccaga ggattttcca ataaaaagga 69780
ggtgtatggt aaatagatgg atagccgagg gttttgtgga tgcaaagttt ggaatggcta 69840
tggaggatgt tggaaatagt tatttcgatg aactcatcaa tagaagcatg attcaaccat 69900
gtaggtttta tagtcatgga gtagtacagt cctgtgtact ccatgatatc atgcgtgata 69960
tcgcaatttc tatttctgca gaggagaatt tcgtattcat gacgaaaggg tttgtctctg 70020
gtattccacc tgaaaacatc cgtcaccttt ctattgatgg gagacaggat tcatacctaa 70080
gctttgatct aagccatgtc cggtcattaa gtttctttta caatcctaaa gagcaactag 70140
cttcactgtg ttcaccacag ctaaggatgc tccgagtgtt ggatctagag tttagtctat 70200
gtcgtgtaac acagaacgac atcagtaaca taggatcatt ttgccactta aggtatctat 70260
ctgtgaagaa aggttcatat atttaccata ttccaagatc catcaggaaa ttgcaaggat 70320
tgcaaacttt aaacctgaaa agatcattaa ttactaaagt gcccgcagaa gtcactgaac 70380
ttcgtagtct ccgcagtctc cggtgtagca cccttggagt ttacagtcac ttcgaattta 70440
ctacccgtga ccccaaaaaa tccttagtaa ctacaatgaa attgcccttg atattgccac 70500
atttgattag cggagacaaa tcctcggagg tggtggcgga gttccgcaag ggtgagcgta 70560
ccaaaaggaa tagggagcct gaaggagtta cagattcttg agcttgtgga tatcgcacga 70620
agcaacaaga aagcagtcca cgagttgggg gaacttactc agctgaaaaa attaggcgtg 70680
gcaggggtat gcttgagcag cttgcttccc cacctccctt cctccacacc ctcaaattga 70740
aaggctccct tcatgaaatc cccagttggg tcggaaagct cgagaagttg gtaaaggttc 70800
agctagtttt caccaaactg aaggatactg aatccataca agtactcggg gaattgcccg 70860
gcctcaaatg cctccgtctt attttaaatg cctacattgg aaaagagcta gttctttgcc 70920
atgggaagtt ccgagggcta aagactcttc gacttgactc tttggaggag ctgagaaagg 70980
ttacattcga ggagaggacc tcgcccaagc tggaaacaat aaccattcag gattgcagct 71040
cggagttagc agtctgcggc acggctaacc tccaaagcct cgagaaaatt aagtattttg 71100
caaaagggaa actggtgaag cgtcctgtgg tgcaagcggg tcagagccaa agtgcgcatc 71160
atcgggagga catcaaagct gcagagataa tagagaaatc tcaaacatca tcactagaga 71220
aaggcgagag ttcacaatca atacctaggc ctgatgtcct tcgtactctt ccacccatct 71280
ctgcaacaac caagctgaag agaagtctgt cctgcccagc atcaacatct atcgtcatgt 71340
gaactataga tcaaagactc tcaacatcaa gtaagtttta tctaattggt atatatacag 71400
cctttcttta ttccattttg tttttttgct gttctcgcaa caatcttatt tgttaaaaaa 71460
cgtttaatat gtgtagatgt tgatgccacc atcttgtaga actcatgacg catcgtagag 71520
ctgtgataat tgtagaactc atgacacatc agagagctgt ggttgttgta gagagctatt 71580
ttcagggact gaacttacct ccatggtggt aactggtatg tgataaacct tgactcttaa 71640
ttgtatttaa ctttacaatt gaaactagtt ttaattctct catcattatt gcttttacta 71700
gcattaacga gtaattgaat gagtatttga ttatcctttt taacataagt aggaaggtag 71760
cccgcacgaa tgtgcgggta tgtatggtag gcggtttcgt ttgagaaact tatatggacg 71820
gaatatcagc cagatggcct gtgccatgtt tcaaaaatat tatttcttca tatttttttt 71880
tcatccgcat tgcttagata tattgttgct aaccgattac atattccagt agtaagattt 71940
ccgctaagtt tgtagttgct gaatgaatat gttgtttggc cggcagttgt tatttcaaaa 72000
gtacattagg tagtctctca agtacaaaaa gaatggcaga tcagaaaagt tttgtccttc 72060
atttattttc tctctgaaag agagtttgct agggtggaga tgtcgtcatg tgaataatca 72120
aatggactac ataagacgca ttgcactgta ctcaacccat tgctgacaaa tggagtgcag 72180
gtccttaacc aggccagata tttgttcagt gtttttgttc aaaaaaaaaa aaaacattct 72240
ggatggaagg ttatttcatt ataaccttta cccttataga ctgcttagta taaacaacaa 72300
ataaggtctc ctgcaaaatt catgtacgct gatatctgtg ttttctgcca tgcagagact 72360
tgcaatctta cagactcatt tatggctgaa tccaaattgg ttgcttggag tctggagctc 72420
catgagatca caatggccac tttttgttgc catgagtggt acatggatcg tgctgctaag 72480
cctttactct gagctagcgg aaaactggct ggctgtatat cgagttcaca atcgccctca 72540
actttgtcaa tgcgatggtg gtcatggata gatgtatgtc atatagcgca aagtggagcc 72600
tccaatgttc gccaaccgta ttgtaaacgt gggaggagaa aatttctcaa gggacaacca 72660
tggaacagag gtgatggagc aaactacttg ctatcatttc agagcacagg ctgaatttta 72720
gctcagataa gagtactaat tcttatttgt ctgtcattga tctatcatac tccttccgtc 72780
cctaaatata agggattttg tttggatatg atatatccta gtataataaa tctagataag 72840
ctccagtcca gattcgttgt attaggatat atcgtatcta accaaaatcc cttatattta 72900
gggacggagg gagtatattt taagaattga atcagatgat tgcttgatta ttacatgatg 72960
atatgtaaaa aatggtattg acaagaagac gtaggagcag ggtggttcat tgctcattga 73020
ggaggtactg ataggtacaa aaatttgaga tttgctccgg tatagcaaaa agtatctcga 73080
gttatgggta cctcacggta ccaaatcgtt tacgattgtt agatctagcg tgaggtatcg 73140
gtatctcaag gactcgatgt actttttgtt agaccggagc atatctttaa aaatttaatg 73200
taaaattcgg tactgagagg tactaaaatt acattagaaa agtgctactt cctattagct 73260
tctcaagaac tgtaaaattg ttcttttaat cttaatcttg gagtactagg atggtaaatt 73320
gagttggtgg ttatttctct actacttttg atgtggtacg aaactaattt gtttctgctc 73380
atgtacgtaa aatggaaaat tttagtgatt attaacctta ttatttttct ctttaatcag 73440
attatttatt ggcaattttt tctacaggac actgttatta tgtgatttta gccataagac 73500
actgcccaaa ctcatttttg gagaaaaaca ctccataata aacgtggtaa tttaccaatg 73560
gacaccacgc cgattaaaat aatgatttcc gattgaggag gagaaagaaa tcgcgtgaaa 73620
tgtcaaaaat gcccttaggc ccacatgtca gctccccatc tctcttcctc tctttctttt 73680
ctttctccct tcttcctgcc gccacgggtg agcagcggcg gcgtgacggc ctgagctggg 73740
acagctcgac gcgcagccgg ggaagcagcg gcgcactgtc ggggacccac caccaacgac 73800
gacgaggcgc agcggccacc cggcgcacgg cgctatggca agtggcgaca ccgagcacac 73860
gagcacggcg gcgacgttct tcctcttcct cgtcggcctc gagcttgacc cagccttgct 73920
ccgccgcacc ggccgcgcca cccttgctgt cgccaccgct agaatctcgc tccagacgtt 73980
gcatcccgga tgccaataat cgggtgccga cgccgttccg cctctgcacc gccccgcacc 74040
acgtcgtgcc tcgtcgtcat tggcggtggg tccctagcgg tgcgctgccg cttcccggcc 74100
acgtgccgag ttgtcctggc tcgagccgtc gtgccgactg tgcgttgcgc cgttgctcac 74160
ccgcggcggg aggaagaagg gagaaagaaa agaaagagat gaagagagat ggaaagctga 74220
tacgtgggct caagggtatt ttcgacattt cacgcgattt ctttctcctc aaccggaaat 74280
cattatttta atcggtgcgg tgtttattgg caaattacct cgtttatgga gtgttttctc 74340
cccaaagtga gtttgggcag cataccacgg ctaaaaccac acaatagcgg tgtcctcaag 74400
caaaaattgc cttatttatt agagggattt atcttttttt agttctgtac aaaaatattt 74460
tttattgtca atgttgccaa aaatttgtga tatcaacatt tttcttttgt tttactagaa 74520
tggacgactg ttttggtgct gcttgcttag acttgctgat tcctccctct ttcctattta 74580
ctcctttttc ttcacttttt tttctgtatt ttggttttct atcctctgtg actacacatt 74640
tgaatcgaac atgctctgtc tctgctcgga atacttatta ttcggccaac ataaatgcat 74700
agggccattt ggaatgcgca cttaaagggt gtgtttagtt cttgaaaaaa gttagaagtt 74760
tatgtgtgta ggaaagtttt cgatgtgata tattgtgatg gaaagttggg agttgggggt 74820
agttggctga actaagcacg gcctaagagt tgtttgaaca tttccatggt gctgtccatt 74880
agcacgtgat taattaaata ttagcttttc tttttcaaaa atagattaat attatttttt 74940
agtaactttc atatagaaaa tttttgtaaa aaaaatactt agtctgcgtt cggatttagg 75000
agatgggaac tcattccctc cacacggaaa acggagcggt ctattagcgc gtgattaatt 75060
aagtattagc tcttttttta aaaaaatgga taaatatgat tttttttaat caactttcgt 75120
atagaaattt tttttaaaaa acagtagagt agtttgaaaa acgtgcgcgc ggaacagagg 75180
gagaggggtt tgcaactaac acagcctaag tttagcagtt tcaaacacaa cgctttccgt 75240
gtcaggccga atccatctca acaataatcc gccattacca ctggtagcac aagtacaacg 75300
cgaggcgcaa ccacagcgca tggccaggcc catcctgcag ctgcgacgac gacgaagact 75360
tgcgtcggag acttggaatc aagtcacggc cggcaaagcg catgccaaaa tatgctacga 75420
cgacgatgac gacgattccg tcagtcacgg cgcgcctgag ggcccatgtt gccagccgac 75480
gccgtccacc cattccttac ttcccccttg ttggttcctc cgccactagc gtccacttct 75540
cctcctgcgt ctagtaccct atcttcacct ttcccgagat tacagaggaa gaggaaggac 75600
gccgaagccg ttgcatcaag cccaggacca ccgtcgaaca cctgctgtgc gcaccgccct 75660
gcgttccttt gcttcaccac cgtgtgtgca cagatcccag ggtgtgcaca gcagcatagg 75720
taagtaaacc ccgaatttct gcagtttctt tttaaaagat aaagcaggag ctctgctatt 75780
caattaagca gggaagatta tttgggtatt ttccgcagtt ttctggaaac catttttttc 75840
cccaaccatt ttcaggtttg gctatttttc ttgcctgtct gttaagctaa acttgcagaa 75900
cttagcataa agttgtaata caagcatagt ttcaccaata tacaaccttt tgttttaagc 75960
tacaacattc acctcaagga agaaaaagta aacttatgaa ttgttggact tgggctgata 76020
tcactcggat ggcggtcccc tcatttttat ttgcatctcc ttacgatttt tttttcagct 76080
ggcatatttg tcaacttcct gtaatctttt tgaagtataa ctcatggaat tcctttgtgt 76140
atattctgtt aacccagtgg ttttatgaca ctaattaaga aagttaacta gttaagagat 76200
tataccgttt gagtttgggc tttggagatg tcactagcta gtggtcgacg gtaggcaagc 76260
cctttctgct ttgctatgaa gaacctccga gctcagtcaa tgtccagaaa aaaagttgga 76320
taactataag tggaagccat ttttggttgg ttttctgcat gttctctttg tccctgtttt 76380
attatgtcat gtaaactttt cattccccag tttgatttat acatgtctat ttgatattgc 76440
agataaatac agttgtagcc tgaaagattg aacttataat ctattgctta aaagtttaaa 76500
gaaagttgtt cagagaagga agataagatg gcggatacag tactcagcat tgcaaagtct 76560
ctggtgggaa gtgctgtaag caaggttgct tcggttgccg cagacaagat ggtcttgctg 76620
ctgggagtgc agaaggagat atgttgagca tctgaattgc agcctaatta attttatttc 76680
ccatttgcat tagatttatt gggaccacgc ttgtgcagag tggtatggta ctcagtttat 76740
ttttaatgat ttatttatat ttttatacca tgttctggag gaatgcattt gcagtttttt 76800
ctataagtat attatttgca acatcttggg gagataaatg tagaggaaag tgaaagtaga 76860
atgcactgga agtccctcta tatacccaaa cacaagtgga catagttttc tcatggcaac 76920
catgttcaat gaaggaatac aaacgaggca gctattaagg acctggtgat aatctaattt 76980
cgacagaaac atggtttcct tcgagtgtaa acaatgtagc atggcatgct taagtcaaat 77040
gcatatacaa tttgacaagg aactattagt ttcagtgctg tgtaattttg ctttttgtag 77100
gatcgaacag aaataactaa gccaactaag cttcacgttc ctagtttgag cttccaattt 77160
gtacatattt gtgatttata atgctatata tacatgtata cagacaagta acgcagtcca 77220
cgtgggatgt tggccatgcc tggataagct gagcaagatt tagacctgtg tcaaaacaat 77280
gtcttgaatt ccgttgaact ttttgtttac aggttcatca aagatgagct acaaacgata 77340
caagcatttt tgattgctgc cgaagcatca aagaaaagca tactattgaa ggtttgggtg 77400
cagcaagtaa gggatctttc ctatgacatc gaagattgcc ttgatgaatt tacagttcat 77460
gtgggcagcc aaaacttgtc gaggcagttg atgaagctaa aggatcgcca tcggattgcc 77520
atccagatcc gcaatctcag gacaagaatt gaagaagtaa gcactaggaa catacgctac 77580
aacttaatag agaatgacct cacctgcacc actactgatg agaggaattt atttatggaa 77640
gacattcgca accaatcagc taacaacatt gaggaagctg atcttgtggg tttttctgga 77700
cccaaaagag agttgcttga tcttatagat gtccatgcca atgacggacc tacaaaagtt 77760
gtatgtgttg tcggtatggg tggtttgggt aagactacta ttgcaaggaa aatttatgaa 77820
agcaaagagg acattgcaaa gaatttttct tgctgtgctt ggattactgt ttcacagtcc 77880
tttgttaggg tggaactact caaggatttg atggtgaaac tttttggaga ggaagtactg 77940
aagaagcggc tgagagaact cgaagggaag gttccacaag tagacgacct ctccagctac 78000
ctcaggacag agttaaatga aaggaggtac tttgttgtgc ttgataacgt gtggagtaca 78060
gattcatgga aatggattaa tagtattgcc ttccctagaa ataacaataa agggagccgg 78120
gtgatagtaa caacaagaga tgttggctta actaaggagt gtacttctga attgcttatc 78180
taccagctta aacccctgga aataagctat gcgaaagagt tgcttctaag gaaagcaaat 78240
aaaacaacag aagatatgga aagtgataaa aagatgagtg acattattac taaaatagta 78300
aagaagtgtg gctatttacc gctggctata ctcacaatag ggggcgtgct tgccaccaaa 78360
gagataagag agtgggaaac cttttatagt cagatacctt cagagcttga gagcaaccca 78420
aaccttgaag caatgagaag gatagtgacc ctaagttaca actacttgcc atctcatctt 78480
aagcaatgct ttttgtatct aagcatattc cctgaggatt ttgaaattaa taggaaccgt 78540
ctggtaaata gatggatggc agaggggttt attaaagcta gggttaatat gactattgaa 78600
gatgttggga aaagttactt taaagaactc atcaaccgta gcatgattca gccatcaaga 78660
gcgggtatac gaggagagtt tcagagttgt cgagtccatg atataatgcg tgatattaca 78720
atttcgattt ctagagagga aaattttgta ttcttaccta ggggcactga ctatgaagca 78780
gtacagggga acactcggca catagcattt caggagagta agtattgctc taagataagc 78840
tttgactgga gcattatacg gtcattaact atgtttgccg agaggcccgt agaactagag 78900
cattcagttt gttcatctca gttgaggatg ttacgggtct tggatctaac agatgcacaa 78960
tttactatca cacaaaatga tgtcaacaat atagtgctct tgtgccactt gaaataccta 79020
cgcattgcaa gatacaataa tgcatcatat atttactcac ttccaaaatc cataggaaga 79080
ctggatggtc tgcagacatt ggacttggat tcgacgaaca tttcaacact gccaactcag 79140
attactaagc ttcgtagtct ccggagcctt agatgcatga agcaatatga tttttcttct 79200
ctcacaacat gtttaactaa cacattttgc ctgcccatga tattcacacc ttccgttagt 79260
acctctgatc gtgctgaaaa aattgctaac ttgcacttgg ccaccaaaag cttccgttca 79320
aaatcaaatg gcgtcaaggt accaaaagga atatgtaggt tgagagactt acaaatattg 79380
ggggtagtgg atattagaag gactagcagt agagtaataa aagagttggg gcagttaagc 79440
aagctgagga aattatatgt ggtaacaaag ggatctacaa agttaaaatg tgagatactc 79500
tatacagcca tccaaaagct ctattcccta caatctctcc atatggatgc tgtgggatgc 79560
acaggtattg gaacacttga gtgcctagat tctgtttcat cgcctcctcc cctactgagg 79620
acactcaagt tgaatggaag tcttgaagag ttgcctaact ggattgagcg gctcactcac 79680
ctgaggaaat tctacttatt gaggaccaaa ctaaaggaag gtaaaaccat gttgatactt 79740
ggggcattgc ccaacctcat gcttcttctt ttttgtcaca atgcttacct tggggagaag 79800
ttagtattca aaacaggagc attcccaaat cttagaacac ttgtgacttt caatttggat 79860
cagcaaagag atattagatt tgaggatggc agctcacccc aattggaaaa gatagaaata 79920
ggcaggtgca gattggaatc agggattatt ggtatcattc accttccaag gctcaaggag 79980
atttcagttc aatacaaagg taaagtggcg atgcttgctc agctggaggg agaagtgaac 80040
gcacacccaa atcgtcccgt gctgcgaatg gcaatggacc gaagtgatca cgaccttgct 80100
ggcgacgcaa aaggatctcc tccctgagca ggagggagag agctcgcaag taatcatgtt 80160
gacaacaaac gacaggtcag tcactctcac cccctacatg gcagcttaat taacttgttt 80220
cctcctcttg ttcagtatta gccatctggt gacatcgatg atttcaactc accctttcat 80280
ctctcttgtt ttcttaacct aacagagaac agatatgcat agctcaaggt ggtttgatca 80340
agtgatcatc tcctccattg gcatctccgg tcgtccctgc ttctgcggct gcgcacacct 80400
cgctgttcca aggaggggtg ctgacataag gtcagggttt gaaatttcgg ttcgaaattt 80460
caccccaacc aaaatgctca catctcgccc gaaattttcg gtttttgcca attttttgtg 80520
aatttggtcg aattttattc aaattcattc aaaatcagtc aaaatttcaa ataatttcgt 80580
acgaaaaatt ctgaaatttc caaaatttcg gttctttcgc tcacctccgg cagaaaacca 80640
agtttcgaaa tttaaaaccc tgcataaggt ggcttccact ttcttccatc gcgtctcact 80700
ctctcggttc ttccctctcg ggtatgaatg gttcaatctg atcttttctc gcgatatgct 80760
actggttcca gcatgagcat ttgaaccagc agcttagaat ttttcatttg atcaggtgtt 80820
ttatttccgc ttcttacctg ggcagtctac ttttccaatt catccagaac agaaaccatg 80880
tttattacac tatagagggg aacaacattt caggcacgat tgtggttttg ttatttcctt 80940
tttggtgtgc acacgaggtg tttgctataa tgtctgaaag agcttgtgta tacatggttg 81000
gttcaacatt atgtgttagt catattttat atcgttgctg acctatcaca tagtgcagga 81060
gtaaaatttt tgctatcttt attgttacct gctgtttatt ttccttttcg taaagaatat 81120
tagctttttg gataacgaaa agaatattat gtagcctgca aaaaggtttg atttgatggt 81180
cactggctag tacaaaagca aagagagatc agacaaaaca aagttgtggt tacattagct 81240
cattgcaatc tatttgctga acatgtgaaa aattaataat ttgtcgtacg tcatgtttgc 81300
tctggcagtc tggctgcagg tggttatttt tttcatatcc attgtcttgg tctacctttt 81360
cctactaaac taggactagg agtagaaaaa agtaattgtt ggttccaagt tcaaaagata 81420
ggcagatgtg agctatggaa cttgcagttc ttgatctcct tcagagcttc tcacctcaca 81480
tatatggatc tctgtttatc ccaaagcaac atttgttaac ctgctagttt cgaattgatc 81540
atctttgtta ttcttcattt attctatttc tctccctaag ggagttttct agggtggata 81600
tgtggaaaat cgaatgcagc tatgctgttc aaaccactgt caaaagatgc aggacaggat 81660
gggcagatgt ttattcagtg ttcttgttca aatgaaacat atactattct ggaagaggtt 81720
ggttaactta cattgtatat aacccttacc ttgttactat cctcttgcaa aatgcatgga 81780
ttggtgaaat catgtttttt gtcatgcaga atctattata ttattaaaac agccttaaaa 81840
aaaggcacca cgtttgctgt ggaggctaga aattcccaca ttaatcgaag aaaaggaaaa 81900
atattttaat cattggatca taatagatct agattttaac ggttaacatc taatacgtat 81960
atagttagaa actagagttg tattgctcac gtaggaatac aaagtggtct gctcagttgc 82020
catcaaattc aaaaaaaaaa ttacggtttg gattccgatt gaaggaataa aggcaggaac 82080
taatatacat gctatattag caatcctaat caagcacgta ccaccaagcg caaggtaata 82140
gttggtgttg atggtgtcct agataattag ttaaggactt tccatgtatt gatctctaat 82200
aatgacatca tcaactaatt aattaattaa tagattagtt ctacagttac accatgcatg 82260
catgtgtatg tgacagtctt ccatgcttgg gcagtatagc tgaatgtttg taactgatat 82320
aaggtgaaga gtcaggatta tctaaataag atacgagtag cttgaagaaa aatccacact 82380
tttattttag aggccaaaaa ctaccatgtt aatcgaaaaa atatattata aagatggcta 82440
aaaaagtgct atcttttatt cgggtggcct aaaaattatc gtctttatga aaaaaatcta 82500
caccattaga taaacctata ttttaaccaa taagatcaat tgataagtat acctaacggc 82560
acattaagta gacaaatact actcaaactt tgaaaaaaca aaagtaccgt acaactaaac 82620
aataatcatg ttcggactct cacactacta aataaactat tttccagacg atggcatttt 82680
ttttcaggcg ggtaggcatc tcggagctaa ttaaatggcc acctacaaaa ataaaaaccg 82740
tgggacgaga ttgttgtcca cccgcgaaaa tatatttttg caaccggaca tgggataaac 82800
acgaatgaaa aaagagtagt tttcagagaa gttttcggtc atcccaaaag aatataatca 82860
atcccctgct tggcactctg tccgctctag gcaataatat tgcatacgac tgaaaaaaga 82920
gtagtgaagc caataaaatt ttaaataccg acaaatatat ctatattaag catttgctaa 82980
acaaaacata ttcgctcgcg agggttccct tttgattacc gacacatgaa aaatataaga 83040
ttaccaatta aaaatattct atagaaggag ctgtgagaag gacccaaggg atgacaaagt 83100
agtaagatag tggttaccac gggaacacgg cctgagggaa ggaaaatgag taaggtgacg 83160
gaaacggttt ctaaatacta tgtaaaaaag aaccctaatc aattaagaat ataatttaga 83220
aataactaaa attgcgaata aaaataagga atatagaaag aagagtctat ctagatcaac 83280
acaacatgag attaattaaa attcagaata caaataaagt aattttgaaa ttgtattttc 83340
aaaattagtc caaaagcaca taaaaaaagt gcaccaagct ttacaaataa aaggtttcga 83400
taggttagtc ctactagggt tgtgggaggg agagctattc aagtgacaaa tagtaggagt 83460
tgtgagaggg cgaccctttg gatgaaaaag agcaaagcaa gggatgcaag gacgacaata 83520
tttagaggga cttcaaacat gatcacgatc atgataagtt tcaatattat taggcgtcga 83580
caacaataaa gaagagatgc ggtgtaagct aacaagggga taagtgataa aggagcggga 83640
agcagtggtt gccatgggag agcgagctga ggagcaaaaa aaaatagaag taatgtggca 83700
gcgatgactt ctaaaaataa taaaaaggaa ctccaactag gataagaggt ttgattttat 83760
aaggtgctga tgacaacaaa atgagagatg gcgggacagg ttgacaacta gacaggtgga 83820
aaataagtaa gaagatgata gttgctatcg gagagtgagc agaggggtgg caaatgaata 83880
aggtagtggt gacaacttcg acaaactatg aaaataaaac cttgacgcga ttttgtaagg 83940
tacctataat gaataagagt agcagtggac atgcgagcgg agggtgcaaa gaagtataga 84000
ggtgtcagtt aacatgggag agcgagctga gagacaacaa atatgtaagt tggtaatggc 84060
atctaaaaac tttaaaaaca gatcaatgat gacgatgaga ttcaagtgca taagtaacaa 84120
ctgtcattgg agggtgacaa atatgaagag gagggacaag atgcaaatga gcaaagggat 84180
agtggcagct gacatgactt cgaaaaccta ttaaaatata gatgcctgat tatttttaag 84240
tcaaagcaga aaaacatgtt ataatcactt tatttaattt cccttaaaaa tcatatcatt 84300
caatcatata agctctctct atttcaaata tatgcctaca tatatgcaat acgatagatt 84360
aatcatagac atagtcaaca agtcagctat tctaattttt atggagattc aaattaaaaa 84420
aatatagata attttgtatc ataatatata tatatataat atttgctagc cgcgcaattg 84480
cgctgctagt tgttttctga agctcaaagt tgaagctgca gtcttcactg tttctggcag 84540
aacccaaatt ggtggcttgg tgctgcatac ctgcagagaa cgcaacggcc actgcttgta 84600
gccttcaagg atgctacatt attgatcatc tcactacggt cccgatcaaa cttctgatcg 84660
agttcgccac cgctgtggac tttgcatatg ttgatggagt ggtgtgtatg aatccagggg 84720
gcagagccac tgccctgcga ccttggggct tgtcaacgag catacagtat actctctccg 84780
tttcttttca tttgacgcta cagtgcaaat cagaatcgtt tttttttttg tttgacgcta 84840
ccagttcatc cgtcctagtc acgtttgtta attttcatcg tagccctcca tctcaacatc 84900
gccacggcgc tgcctcgtcc tcatcgcgac cggactcgct cgccactgtc tcctcctccc 84960
acgcgccgct gccgttcatg cctcccgtgc gccgccgccg caccccaatt tggcggcaac 85020
ccaccccgag ctgtggcctg tggggcaagc aagagtcggg cagcatggcg cccagggagc 85080
cggacgtcgg gcttgctgct gccgccactg ttgttcttgc cactcggctg aaaccaagca 85140
gaacgtactg catctgcaga tgacatcgtc gttattgccg ttcggctgga gatcaaccag 85200
aaccagggct tctcctctcc ttctgcttaa attgtaaatt gggcaaaacc aggcaattag 85260
gcaatgaaaa ttggaggaaa tgagaaaaaa aaaaattaaa caagctggaa gttggagaag 85320
gcgagatcga ggagctcact tacagagagg gtgacgagtg aggagccgag gtccggggag 85380
gacatgctgc ttggagacga gaaaggagga ggactgaatc gagaaggacc gcttgatctc 85440
aggctccaac gccgctttca ccgggaatgt cggagaagaa agggcgacgc ggcgtccggg 85500
aagagcgttg tgcagcgggt tcctcctcct gtggtcgcga gcagcggcgt ttgggagacg 85560
acgagcgaac cggcgtcgag gcagcgcggg ggagatggca agggagccgg cgtggaggga 85620
gacggccggg ccgcgcgcac tgtgctcctc cctgtcgtcc aaaagggatg aagggcaaaa 85680
tagtaaaatc gctctatcct taattttgtc gatgctgtcg gaagcgtcat atatattgaa 85740
acggagggag taatattttg ctgttatttc agtgattaaa aacgaaaatt ttaaagaaaa 85800
cattgaatgc aattagatct gctcagattc tcaaaatttc tttcactccg ccactgcttg 85860
aatcgatatg tcatatgcta caaattaagt ggattggaga tgtacatgcg ctcgaatgat 85920
ctcggttata ttgtaattgt ggagtagcaa ctttatattg ggtataacca tggaacaagg 85980
gtgacgatga atcaaactat ttgctgtcat ttgaagagct ccagcataca tatttcacta 86040
tatcagtttt ttattttgag ttgaaagttt tcaaaatttt tcttgcaaat tgaaattttc 86100
aaattttgag ttgagagttg tcaaaattta aatcgaaagt taaaaagttt tcaaatataa 86160
cttaaaaatt ttcaatctat tagtaaaaaa aattctccaa aaaaataaaa aatctttctt 86220
taattaccct cattatcctt aactaattga aatagttatg ttaaccccaa cacgtgcgcg 86280
tccgcatacg cggcagctag tcttctcgtt tccatacatc gtttacacct gctagtgcta 86340
tggctgcttg aggatatggc aaagcttaaa agatgatgtt aacacggtca catggagatg 86400
caggaacatg catatacagg gcactccatt ttctgttttg tctcaatctt atgtagagct 86460
aatatggaca ataccagtag ttacatcttt ctgtcaacta ggcatcctac ccacgtgttg 86520
ctcaatcgat gtaaacttat ttattaaatc aatgcaatca cgtcgcgttg gttcatctat 86580
ttacacttgg ttataatatg atccctgtgg taaaatataa gaacttctat gattctagta 86640
aaaagtgata tctcatagta cctactcaag gatcgtaaaa ttgctcattt ataatagtac 86700
ttgtctcacc gatcattttc gattcaattc ttttctatct tacattctct tccacgctca 86760
tccattcctt ctttataaag agaaaataaa gttaacctta atatctctgg ggaataactt 86820
atatttcagc ggaggtagta agaaaataca aacaaccaac taaagtactt gagtaaaggg 86880
gtgcaatatt tatgtggcat aaaaaacact tatcttttta ttatttttat tatagttcgt 86940
ggctgaccat taattgttct agaaggctag tgctagtacc tccgtccttt tagggttgga 87000
catgggtatt aaaaattagg tgattcccca cgctttaatg tagaaattcc taagtaactt 87060
ttaatatttt tctaacaatc gagctaaaag taaaaattat attagttatg aacaacaaat 87120
aaaactggat tgtcaaacta tgttcagaga taaatacatt taagtagatt ctgtataaga 87180
gattgtagat gaaatatgat ataccgcaca ttgattatat ggataaatat atgttaagta 87240
ttataaaaat gatggatata tatgtgtgtg ttaaaaatat tgtgaaaaat aatgtgaacg 87300
aagatgattg gatgttgatt tgtagaattc agtgaattat agatgatatt gcatgcttgc 87360
ataagttttg tatgtaatta ttgctagtgg atgatgaggt ggcatgtttg catgttgagc 87420
tttagaatta gtggactata actttattgt aagatatgat taagtacaat taaatgagga 87480
atataatgat taattgagaa gtagagatag gtgagagaaa ttgaatggtg tagggtagtg 87540
attggttggg aagataatgt tgatgaaaaa agttatattt tgggacaaat cttgaatgct 87600
aaaaactgtt atattttagg acggatgtag taaaatatat tgatctatca ctacgaatat 87660
gaatggaggg ttactagggt ttagattgat tgtactaggt tgtgtctatt ctattactag 87720
gttgttatat tttaaaatag agagtatcaa actacttgtt gaaccaatag cggtgttaag 87780
agcctctttg gagagcttct catagaatat ctaacttccc aaacagtgta aattctgacc 87840
tacattctaa gaagctagta cgaaaaccaa gtttgcagat actcagaagc tggctaccaa 87900
atagtagctt ctcagaatct taagctttcc aaatagaacc taaatctcct aatctctttc 87960
tccacccaat ggcttctaca tgtatgtgtg gtggtgacaa tttccccaaa ccccatggcc 88020
tctcgcgtat agtaatcggt catgccttca ttgacgagca agctcgtctc attaagtctc 88080
gattcgtttg gactcattat cttaaggaac cattccagcc tagcttagct ccttaatctt 88140
aacgaaataa aagtttagct tggcttggct cgcgagctga tttgttaagc ttttgttagg 88200
cttgttaaca catttatata tcctttataa ttttaaattt accattttat catatattac 88260
catagtaata acactaaata taaatagctt ggatataaat gaaagactaa ataatacaaa 88320
gtatatgtag ttatgataat ctcttgtact actaacaagc taaacaagca attggcgagt 88380
tgccttgtta agctcacaag ctaaaagcct atctccgctt gattcataat gactacaagt 88440
tcgaacaaaa ctcactacaa accaatccgg ggcacggtct ttgagctttc tttccgcccc 88500
agtgacactt gtgttgagat gaatcaacgt gacactagct accgtgagat gtagtgaaga 88560
gacatgtcag tacactacaa tagatctagg cttccttgat ttattccctt gtgacgaata 88620
gggagctcat cattcatcta tgaccaattc gtaaaaaata tttgttgtaa acttcaccct 88680
cggccgattc tagtgataac tggaattctt ggtcataata tatgttcaat tacgggtgga 88740
caggaaggtt atggctcgtt agctcgctc 88769
<210> 2
<211> 368
<212> DNA
<213> Oryza sativa
<400> 2
agcaaaaagt tggtttcagt ggtgttggta tttcttacga ccacagataa atccgcaagc 60
gcacggaata ccgcagtagc acttcacatt cgggtgaccc caaaaggata tcgaactcag 120
ggtacgtgta taatctacct aagactaaga caatctaagg acaacaactg atggtaggca 180
ggctagacta gagaggactt tctatgtgtt aattatctac gctaagcaaa ggtaatttcc 240
tatctgttgc ttcaggcacc aacccctgct gatctgccag cgtggttccg gctatagctc 300
tatgatgtat ccgaacgttg aggattacta agacgggatt cagggctgtc accacctgct 360
atctacct 368
<210> 3
<211> 21
<212> DNA
<213> Oryza sativa
<400> 3
agcaaaaagt tggtttcagt g 21
<210> 4
<211> 19
<212> DNA
<213> Oryza sativa
<400> 4
aggtagatag caggtggtg 19
<210> 5
<211> 21
<212> DNA
<213> Oryza sativa
<400> 5
tggcatctct cagcacattc c 21
<210> 6
<211> 20
<212> DNA
<213> Oryza sativa
<400> 6
tgcacaatgg atgggtcaga 20
<210> 7
<211> 157
<212> DNA
<213> Oryza sativa
<400> 7
tggcatctct cagcacattc cagcaggtaa atatacaaat gcagcaatgt agtgtgttta 60
cctcatgaac ttgatcaatt tgcttacaat gttgcttgcc gttgcagatg tggattgcca 120
aggctgagta cggcgagtct gacccatcca ttgtgca 157
Claims (10)
1.一种水稻抗稻瘟病基因Pisj，其特征在于：所述水稻抗稻瘟病基因Pisj为显性基因，其核苷酸序列如序列表SEQ ID NO：1所示。
2.一种水稻抗稻瘟病基因Pisj的共分离分子标记，其特征在于：所述共分离分子标记为SNP标记S6-43-32，所述SNP标记S6-43-32为显性标记，其PCR扩增序列如序列表SEQ IDNO：2所示。
3.一种权利要求2所述水稻抗稻瘟病基因Pisj的共分离分子标记S6-43-32的PCR扩增引物对，其特征在于，所述引物对的上游引物核苷酸序列如SEQ ID NO：3所示，下游引物核苷酸序列如SEQ ID：4所示，利用该引物对对待测水稻材料进行扩增，若能扩增出分子量为368bp的条带，则待测水稻含有水稻抗稻瘟病基因Pisj，若没有扩增出分子量为368bp的条带，则待测水稻不含水稻抗稻瘟病基因Pisj。
4.根据权利要求3所述的引物对，其特征在于，为了提高S6-43-32共分离分子标记的准确性，在PCR扩增体系中加入OsACTIN1基因(LOC_Os03g50885)的引物对，扩增OsACTIN1基因作为PCR结果的阳性对照，OsACTIN1基因的PCR扩增引物对序列如SEQ ID NO：5和SEQ IDNO：6所示。
5.根据权利要求4所述的引物对，其特征在于，
权利要求4所述的两组引物对对待测水稻基因组DNA进行PCR扩增，电泳检测扩增产物，若电泳显示只有一条条带，则待测水稻不含有水稻抗稻瘟病基因Pisj；若电泳显示为两条带，则待测水稻含有水稻抗稻瘟病基因Pisj。
6.一种检测水稻抗稻瘟病基因Pisj的方法，其特征在于，
对待测水稻进行权利要求2所述的水稻抗稻瘟病基因Pisj共分离分子标记的检测，若有，则含有水稻抗稻瘟病基因Pisj，若没有，则不含有水稻抗稻瘟病基因Pisj。
7.根据权利要求6所述的检测方法，其特征在于，利用权利要求4所述的两组引物对对待测水稻基因组DNA进行PCR扩增，电泳检测扩增产物，若电泳显示只有一条条带，序列长度为156bp，则为OsACTIN1基因的PCR扩增产物，待测水稻不含有水稻抗稻瘟病基因Pisj；若电泳显示为两条带，序列长度分别为368bp、156bp，则含有水稻抗稻瘟病基因Pisj和OsACTIN1基因。
8.根据权利要求7所述的检测方法，其特征在于，所述PCR扩增体系为15μL，包括：2×rTaq PCR MasterMix 7.5μL，上、下游引物各0.5μL，DNA模板1μL，ddH2O 4.5μL；所述PCR扩增的程序为：95℃预变性5min；95℃变性30s，61℃退火30s，72℃延伸30s，29个循环；72℃深度延伸7min；16℃保存；扩增产物在1.5％的琼脂糖凝胶上200V电泳分离。
9.一种水稻辅助育种方法，其特征在于，通过权利要求6、7或权利要求8任一项所述的方法确定待测水稻稻瘟病抗性性状。
10.权利要求1所述水稻抗稻瘟病基因Pisj、权利要求2所述共分离分子标记、权利要求3或4所述任一引物对在水稻抗稻瘟病育种中的用途。
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115873867A (zh) * | 2022-09-28 | 2023-03-31 | 云南省农业科学院农业环境资源研究所 | 一种抗稻瘟病基因Pi69及其编码蛋白与应用 |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1840696A (zh) * | 2006-01-26 | 2006-10-04 | 中国水稻研究所 | 水稻广谱抗稻瘟病基因的分子鉴别及转育技术 |
| CN103146695A (zh) * | 2013-03-01 | 2013-06-12 | 福建省农业科学院生物技术研究所 | 一种水稻抗稻瘟病基因Pi9的功能分子标记及其应用 |
| CN103320437A (zh) * | 2013-07-10 | 2013-09-25 | 广东省农业科学院植物保护研究所 | 稻瘟病抗性基因Pi2基因特异性分子标记Pi2SNP及制备与应用 |
| CN104611332A (zh) * | 2015-01-27 | 2015-05-13 | 湖北省农业科学院粮食作物研究所 | 水稻抗稻瘟病基因Piz-t特异性SNP分子标记引物及应用 |
| CN107916300A (zh) * | 2018-01-19 | 2018-04-17 | 福建省农业科学院生物技术研究所 | 一种稻瘟病抗性基因座Pi2/9功能基因分子标记及其应用 |
| CN107988419A (zh) * | 2018-01-19 | 2018-05-04 | 福建省农业科学院生物技术研究所 | 一种稻瘟病抗性基因Pigm功能特异性分子标记及其应用 |
-
2018
- 2018-08-20 CN CN201810946094.XA patent/CN109182342B/zh active Active
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1840696A (zh) * | 2006-01-26 | 2006-10-04 | 中国水稻研究所 | 水稻广谱抗稻瘟病基因的分子鉴别及转育技术 |
| CN103146695A (zh) * | 2013-03-01 | 2013-06-12 | 福建省农业科学院生物技术研究所 | 一种水稻抗稻瘟病基因Pi9的功能分子标记及其应用 |
| CN103146695B (zh) * | 2013-03-01 | 2014-12-10 | 福建省农业科学院生物技术研究所 | 一种水稻抗稻瘟病基因Pi9的功能分子标记及其应用 |
| CN103320437A (zh) * | 2013-07-10 | 2013-09-25 | 广东省农业科学院植物保护研究所 | 稻瘟病抗性基因Pi2基因特异性分子标记Pi2SNP及制备与应用 |
| CN104611332A (zh) * | 2015-01-27 | 2015-05-13 | 湖北省农业科学院粮食作物研究所 | 水稻抗稻瘟病基因Piz-t特异性SNP分子标记引物及应用 |
| CN107916300A (zh) * | 2018-01-19 | 2018-04-17 | 福建省农业科学院生物技术研究所 | 一种稻瘟病抗性基因座Pi2/9功能基因分子标记及其应用 |
| CN107988419A (zh) * | 2018-01-19 | 2018-05-04 | 福建省农业科学院生物技术研究所 | 一种稻瘟病抗性基因Pigm功能特异性分子标记及其应用 |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115873867A (zh) * | 2022-09-28 | 2023-03-31 | 云南省农业科学院农业环境资源研究所 | 一种抗稻瘟病基因Pi69及其编码蛋白与应用 |
| CN115873867B (zh) * | 2022-09-28 | 2023-06-23 | 云南省农业科学院农业环境资源研究所 | 一种抗稻瘟病基因Pi69及其编码蛋白与应用 |
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