CN109182112A - A kind of small-sized microbial inoculant installation for fermenting - Google Patents

A kind of small-sized microbial inoculant installation for fermenting Download PDF

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Publication number
CN109182112A
CN109182112A CN201811239546.7A CN201811239546A CN109182112A CN 109182112 A CN109182112 A CN 109182112A CN 201811239546 A CN201811239546 A CN 201811239546A CN 109182112 A CN109182112 A CN 109182112A
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China
Prior art keywords
fermentor
sealing cover
installation
small
gas
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Withdrawn
Application number
CN201811239546.7A
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Chinese (zh)
Inventor
李博文
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Zhengzhou Welt Biotechnology Co Ltd
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Zhengzhou Welt Biotechnology Co Ltd
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Priority to CN201811239546.7A priority Critical patent/CN109182112A/en
Publication of CN109182112A publication Critical patent/CN109182112A/en
Withdrawn legal-status Critical Current

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/38Caps; Covers; Plugs; Pouring means
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/06Nozzles; Sprayers; Spargers; Diffusers
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/02Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by impregnation, e.g. using swabs or loops
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M37/00Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
    • C12M37/04Seals
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
    • C12M41/18Heat exchange systems, e.g. heat jackets or outer envelopes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/26Means for regulation, monitoring, measurement or control, e.g. flow regulation of pH

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Sustainable Development (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Thermal Sciences (AREA)
  • Clinical Laboratory Science (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The present invention provides a kind of small-sized microbial inoculant installation for fermentings, belong to field of biotechnology, including fermentor;Sealing cover is set above the fermentor;Gas-guide tube is passed through on the left of the sealing cover, right side passes through perfusion tube, and center passes through cleaning pipe;The gas-guide tube upper end connects outside air by setting air filter, and impact bag is arranged in gas-guide tube lower end;Flow rotation disk is set below the impact bag;The perfusion tube upper end connects transfer needle by infusion pump;The cleaning sprayer being arranged below the cleaning pipe connection sealing cover;Sewage pipe is arranged in the fermentor lower right corner;Blowdown valve is set on the sewage pipe;The present invention heats fermentor internal environment by the way that heater is arranged, and improves fermentation efficiency;Sensor is monitored yeasting;Filter device filters the air, and flow rotation disk stirs material, accelerates microbial fermentation;Transfer needle is inoculated with microorganism;Cleaning sprayer cleans fermentor.

Description

A kind of small-sized microbial inoculant installation for fermenting
Technical field
The present invention relates to a kind of installation for fermenting, specifically a kind of small-sized microbial inoculant installation for fermenting.
Background technique
Microbial fermentation refers to using microorganism, and under appropriate conditions, raw material is turned by specific metabolic pathway Turn to the process for the product that necessary for human is wanted.Microbial fermentation production level depends primarily on the hereditary capacity and training of strain itself The condition of supporting;The factor that fermentation quality is influenced other than the production performance of strain further includes the proportion of culture medium, the quality of raw material, goes out The condition of bacterium, the environment of fermentation and control of process etc., there is close connection;
Existing small-sized fermentation classification inoculation apparatus lacks to the temperature of fermentation and the monitoring of pH value, cannot accomplish directly to be inoculated with, not have also There is good cleaning waste removal equipment, is unfavorable for controlling fermentation process and being produced.
Summary of the invention
In view of the above shortcomings of the prior art, the technical problem to be solved in the present invention is to provide a kind of temperature to control, conveniently Inoculation, small-sized microbial inoculant installation for fermenting easy to clean.
In order to solve the above technical problems, the present invention provides the following technical scheme that
A kind of small-sized microbial inoculant installation for fermenting, including fermentor, sealing cover, gas-guide tube, perfusion tube, transfer needle, cleaning spray Head and blowdown valve;Sealing cover is set above the fermentor;Gas-guide tube is passed through on the left of the sealing cover, right side passes through perfusion tube, Center passes through cleaning pipe;The gas-guide tube upper end connects outside air by setting air filter, and the setting of gas-guide tube lower end is slow Rush capsule;Flow rotation disk is set below the impact bag;The perfusion tube upper end connects transfer needle by infusion pump;The cleaning Pipe connects the cleaning sprayer being arranged below sealing cover;Feed pipe is arranged in the fermentor upper left side, and sewage pipe is arranged in the lower right corner;Institute It states and blowdown valve is set on sewage pipe.
As the further improvement project of the present invention: also setting up micropore filtering film on the gas-guide tube;The micro porous filtration Film is between air filter and sealing cover.
As the further improvement project of the present invention: also setting up fluid-delivery valve on the perfusion tube;The fluid-delivery valve is located at defeated Between liquid pump and sealing cover.
As the further improvement project of the present invention: the perfusion tube lower end connected pan;The pan of crossing is close to hair The bottom of fermentation tank.
As the further improvement project of the present invention: installing heater below the fermentor.
As the further improvement project of the present invention: the fermenter base setting temperature sensor and PH sensor;Institute State the left side that PH sensor is located at temperature sensor.
As further improvement project of the invention: display is arranged in the sealing cap surface.
Compared with prior art, the beneficial effects of the present invention are:
The present invention heats fermentor internal environment by the way that heater is arranged, and improves fermentation efficiency;Temperature sensor and PH Sensor is monitored the yeasting inside fermentor;Air filter and micropore filtering film filter the air and lead to It crosses gas-guide tube and enters fermentor, flow rotation disk stirs material in fermentor, accelerates microbial fermentation;Transfer needle is defeated to infusion pump The microorganism sent is inoculated with;Cleaning sprayer carries out cleaning to fermentor and is discharged by blowdown valve.
Detailed description of the invention
Fig. 1 is a kind of structural schematic diagram of small-sized microbial inoculant installation for fermenting;
Fig. 2 is a kind of structural schematic diagram of flow rotation disk in small-sized microbial inoculant installation for fermenting;
Fig. 3 is the structural schematic diagram of enlarged drawing A in small-sized microbial inoculant installation for fermenting a kind of;
In figure: 1- fermentor, 2- sealing cover, 3- heater, 4- temperature sensor, 5-PH sensor, 6- gas-guide tube, 7- air mistake Filter, 8- micropore filtering film, 9- impact bag, 10- flow rotation disk, 11- perfusion tube, that 12- crosses pan, 13- fluid-delivery valve, 14- is defeated Liquid pump, 15- transfer needle, 16- cleaning sprayer, 17- cleaning pipe, 18- delivery valve, 19- feed pipe, 20- sewage pipe, 21- blowdown valve, 22- display.
Specific embodiment
The technical solution of the patent is explained in further detail With reference to embodiment.
The embodiment of this patent is described below in detail, examples of the embodiments are shown in the accompanying drawings, wherein from beginning to end Same or similar label indicates same or similar element or element with the same or similar functions.Below with reference to attached The embodiment of figure description is exemplary, and is only used for explaining this patent, and cannot be understood as a limitation of this patent.
In the description of this patent, it is to be understood that term " center ", "upper", "lower", "front", "rear", " left side ", The orientation or positional relationship of the instructions such as " right side ", "vertical", "horizontal", "top", "bottom", "inner", "outside" is based on the figure Orientation or positional relationship, be merely for convenience of description this patent and simplify description, rather than the device of indication or suggestion meaning or Element must have a particular orientation, be constructed and operated in a specific orientation, therefore should not be understood as the limitation to this patent.
In the description of this patent, it should be noted that unless otherwise clearly defined and limited, term " installation ", " phase Even ", " connection ", " setting " shall be understood in a broad sense, for example, it may be being fixedly linked, being arranged, may be a detachable connection, set It sets, or is integrally connected, is arranged.For the ordinary skill in the art, above-mentioned art can be understood as the case may be The concrete meaning of language in this patent.
Embodiment one
Please refer to Fig. 1-3, present embodiments provide a kind of small-sized microbial inoculant installation for fermenting, including fermentor 1, sealing cover 2, Gas-guide tube 6, perfusion tube 11, transfer needle 15, cleaning sprayer 16 and blowdown valve 21;Sealing cover 2 is set above the fermentor 1;Institute It states and passes through gas-guide tube 6 on the left of sealing cover 2, right side passes through perfusion tube 11, and center passes through cleaning pipe 17;6 upper end of gas-guide tube is logical It crosses setting air filter 7 and connects outside air, impact bag 9 is arranged in 6 lower end of gas-guide tube;Air-flow is set below the impact bag 9 Rotating disk 10;11 upper end of perfusion tube connects transfer needle 15 by infusion pump 14;The cleaning pipe 17 connects 2 lower section of sealing cover The cleaning sprayer 16 of setting;Feed pipe 19 is arranged in 1 upper left side of fermentor, and sewage pipe 20 is arranged in the lower right corner;The sewage pipe Blowdown valve 21 is set on 20;For air by entering fermentor 1 by gas-guide tube 6 after the filtering of air filter 7, impact bag 9 buffers gas Flow velocity is flowed, the gas of flowing drives flow rotation disk 10 to rotate, overturns to the material in fermentor 1, improves microorganism hair The efficiency of ferment;Infusion pump 14 extracts the microorganism after fermentation by perfusion tube 11 out, and is inoculated with by transfer needle 15;Fermentor The clear water that waste residue in 1 is conveyed by cleaning pipe 17 and delivery valve 18, is sprayed on the inner wall of fermentor 1 by cleaning sprayer 16, Sewage pipe 20 and blowdown valve 21 by after cleaning impurity and sewage fermentor 1 is discharged;
Heater 3 is installed below the fermentor 1;1 bottom of the fermentor setting temperature sensor 4 and PH sensor 5;It is described PH sensor 5 is located at the left side of temperature sensor 4;Display 22 is arranged in 2 surface of sealing cover;Heater 3 is to fermentor 1 Tank bottom is heated, and is guaranteed 1 internal temperature of fermentor, is accelerated the process of microbial fermentation, temperature sensor 4 and PH sensor 5 The yeasting in fermentor 1 is monitored, and the data of monitoring are shown on display 22.
Embodiment two
Fig. 1-3 is please referred to, a kind of small-sized microbial inoculant installation for fermenting, including micropore filtering film 8 are present embodiments provided;It is described Micropore filtering film 8 is also set up on gas-guide tube 6;The micropore filtering film 8 is between air filter 7 and sealing cover 2;Micropore mistake Filter membrane 8 crosses the impurity in air filtering, purifies air.
Embodiment three
Fig. 1-3 is please referred to, present embodiments provides a kind of small-sized microbial inoculant installation for fermenting, including cross pan 12 and fluid-delivery valve 13;Fluid-delivery valve 13 is also set up on the perfusion tube 11;The fluid-delivery valve 13 is between infusion pump 14 and sealing cover 2;It is described defeated 11 lower end of liquid pipe connected pan 12;The pan 12 of crossing is close to the bottom of fermentor 1;The control of fluid-delivery valve 13 inoculation, infusion;It crosses Pan 12 is placed impurity and is entered in perfusion tube 11, and then blocks transfer needle.
The working principle of the invention is: raw material is added in fermentor 1 from feed pipe 19, and heater 3 carries out fermentor 1 Heating, temperature sensor 4 and PH sensor 5 incude temperature and pH value in fermentor 1, and show on display 22, air Fermentor 1,9 buffer gas flow flow velocity of impact bag, stream are entered by gas-guide tube 6 after filtering by air filter 7 and micropore filtering film 8 Dynamic gas drives flow rotation disk 10 to rotate, and overturns to the material in fermentor 1, improves the efficiency of microbial fermentation; Infusion pump 14 extracts the microorganism after fermentation by perfusion tube 11 out, and is inoculated with by transfer needle 15;Waste residue in fermentor 1 The clear water conveyed by cleaning pipe 17 and delivery valve 18, is sprayed on the inner wall of fermentor 1,20 He of sewage pipe by cleaning sprayer 16 Blowdown valve 21 by after cleaning impurity and sewage be discharged fermentor 1.
The preferred embodiment of the patent is described in detail above, but this patent is not limited to above-mentioned embodiment party Formula within the knowledge of one of ordinary skill in the art can also be under the premise of not departing from this patent objective It makes a variety of changes.

Claims (7)

1. a kind of small-sized microbial inoculant installation for fermenting, including fermentor (1), sealing cover (2), gas-guide tube (6), perfusion tube (11), transfer needle (15), cleaning sprayer (16) and blowdown valve (21);It is characterized in that: sealing cover is arranged above the fermentor (1) (2);Gas-guide tube (6) are passed through on the left of the sealing cover (2), right side passes through perfusion tube (11), and center passes through cleaning pipe (17);It is described Gas-guide tube (6) upper end connects outside air by setting air filter (7), and impact bag (9) are arranged in gas-guide tube (6) lower end;Institute It states and flow rotation disk (10) is set below impact bag (9);Perfusion tube (11) upper end connects transfer needle by infusion pump (14) (15);The cleaning sprayer (16) being arranged below cleaning pipe (17) connection sealing cover (2);Fermentor (1) upper left side is set It sets feed pipe (19), sewage pipe (20) are arranged in the lower right corner;Blowdown valve (21) are set on the sewage pipe (20).
2. a kind of small-sized microbial inoculant installation for fermenting according to claim 1, it is characterized in that: on the gas-guide tube (6) Also set up micropore filtering film (8);The micropore filtering film (8) is located between air filter (7) and sealing cover (2).
3. a kind of small-sized microbial inoculant installation for fermenting according to claim 1, it is characterized in that: on the perfusion tube (11) Also set up fluid-delivery valve (13);The fluid-delivery valve (13) is located between infusion pump (14) and sealing cover (2).
4. a kind of small-sized microbial inoculant installation for fermenting according to claim 1 or 3, it is characterized in that: the perfusion tube (11) lower end connected pan (12);Cross pan (12) are close to the bottom of fermentor (1).
5. a kind of small-sized microbial inoculant installation for fermenting according to claim 1, it is characterized in that: under the fermentor (1) Side's installation heater (3).
6. a kind of small-sized microbial inoculant installation for fermenting according to claim 1 or 5, it is characterized in that: the fermentor (1) Temperature sensor (4) and PH sensor (5) is arranged in bottom;The PH sensor (5) is located at the left side of temperature sensor (4).
7. a kind of small-sized microbial inoculant installation for fermenting according to claim 1, it is characterized in that: the sealing cover (2) table Display (22) are arranged in face.
CN201811239546.7A 2018-10-24 2018-10-24 A kind of small-sized microbial inoculant installation for fermenting Withdrawn CN109182112A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201811239546.7A CN109182112A (en) 2018-10-24 2018-10-24 A kind of small-sized microbial inoculant installation for fermenting

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201811239546.7A CN109182112A (en) 2018-10-24 2018-10-24 A kind of small-sized microbial inoculant installation for fermenting

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112300919A (en) * 2020-10-21 2021-02-02 南京延长反应技术研究院有限公司 Micro-interface fermentation system and fermentation method thereof

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112300919A (en) * 2020-10-21 2021-02-02 南京延长反应技术研究院有限公司 Micro-interface fermentation system and fermentation method thereof

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Application publication date: 20190111

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