CN109174226A - WB tests transferring film cooler - Google Patents
WB tests transferring film cooler Download PDFInfo
- Publication number
- CN109174226A CN109174226A CN201811159510.8A CN201811159510A CN109174226A CN 109174226 A CN109174226 A CN 109174226A CN 201811159510 A CN201811159510 A CN 201811159510A CN 109174226 A CN109174226 A CN 109174226A
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- China
- Prior art keywords
- heat
- conducting plate
- transferring film
- tests
- hole
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L7/00—Heating or cooling apparatus; Heat insulating devices
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- Health & Medical Sciences (AREA)
- Clinical Laboratory Science (AREA)
- Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
Abstract
The present invention relates to experimental equipment technical fields, and in particular to a kind of WB experiment transferring film cooler；The cavity for storing refrigerant is equipped with including heat-conducting plate, in the heat-conducting plate；Cooler construction provided by the invention is simple, easy to use, small in size, reusable, can improve the transferring film efficiency and effect of WB experiment.
Description
Technical field
The present invention relates to experimental equipment technical fields, and in particular to a kind of WB experiment transferring film cooler.
Background technique
Protein immunoblotting (Western Blot, WB) experiment is molecular biology, biochemistry and immunogenetics
In a kind of common experimental method.The basic principle is that by specific antibody to the processed cell of gel electrophoresis or biological group
Specific protein in tissue samples develops, and then obtains specific protein in institute by the position of analysis development and development depth
The information of expression in the cell or tissue of analysis.WB experiment specifically includes sample acquisition, Protein Extraction, protein concentration survey
Fixed, SDS-PAGE electrophoresis, transferring film, closing, immune response, chemiluminescence and Image Acquisition.Wherein, transferring film is by albumen
Matter is transferred to the process on film from polyacrylamide gel.Since transferring film step can discharge a large amount of heat, distribute not in time
Heat will affect the transferring film efficiency of protein, therefore radiating treatment is an important factor for influencing transferring film result.
Currently, common heat dissipating method has the following two kinds in transferring film step: one is ice chest is placed in transferring film slot, secondly
It is to place ice cube outside transferring film slot.The former, which is disadvantageous in that, reduces the volume that can be used for placing buffer in slot, unfavorable
In the progress of experiment, especially in small-sized transferring film slot, this drawback is become apparent.The latter is disadvantageous in that: 1, needs
A large amount of ice cubes are prepared in advance；2, need to provide sufficiently large container so that transferring film slot to be put into container；3, ice at the end of testing
Block partial melting, slot it is outer throughout water mark need to clear up.
Summary of the invention
According to the above-mentioned deficiencies of the prior art, it is an object of the invention to propose a kind of WB experiment transferring film cooler, knot
Structure is simple, easy to use, small in size.
In order to solve the above-mentioned technical problem, the technical solution adopted by the present invention are as follows: a kind of WB experiment transferring film cooler, packet
Heat-conducting plate is included, the cavity for storing refrigerant is equipped in the heat-conducting plate.
Preferably, the heat-conducting plate wooden partition is equipped with the through-hole for being connected to cavity and atmospheric environment.
Preferably, side wooden partition neighbouring or ipsilateral with side where through-hole with through-hole on the heat-conducting plate is equipped with hook.
Preferably, the opening of the hook is directed toward ground, and hook is arranged symmetrically at left and right sides of heat-conducting plate.
Preferably, the heat-conducting plate is made of materials such as the aluminium, copper, alloy of good heat conductivity.
Preferably, the heat-conducting plate is rectangular-shape, and the plate thickness of heat-conducting plate is 0.05~0.2mm.
Preferably, the refrigerant is isopropanol or water.
Preferably, the hole wall of the through-hole is equipped with internal screw thread, and heat-conducting plate further includes screw, screw be equipped with external screw thread with
Internal screw thread in through hole wall is adapted.
The beneficial effects of the present invention are: cooler provided by the invention uses the side of Metal Substrate heat-conducting plate combination refrigerant
Formula replaces traditional ice cube, replaces the heat exchange method of convection current to radiate the liquid in transferring film slot to conduct, with volume
Accounting is small, the advantage of good heat dissipation effect.The configuration of the present invention is simple, it is easy to use, it is small in size, it is reusable, WB reality can be improved
The transferring film efficiency and effect tested.
Detailed description of the invention
Fig. 1 is the structural schematic diagram of the embodiment of the present invention；
Fig. 2 be Fig. 1 in A-A to the schematic diagram of the section structure.
Specific embodiment
Below by the description to embodiment, the shape of for example related each component of a specific embodiment of the invention, structure
It makes, the mutual alignment between each section and connection relationship, the effect of each section and working principle, manufacturing process and the side of operating with
Method etc., is described in further detail, completeer to help those skilled in the art to have inventive concept of the invention, technical solution
Whole, accurate and deep understanding.
A kind of WB tests transferring film cooler, refering to fig. 1 with 2: including heat-conducting plate 10, being equipped with and be used in the heat-conducting plate 10
Store the cavity 11 of refrigerant.
In use, can be freezed heat-conducting plate 10 in advance several hours, be placed in transferring film slot, the cooling for transferring film slot
Radiating treatment.In fact, heat-conducting plate 10 can be designed as totally enclosed type in advance in the cavity 11 of heat-conducting plate 10 by refrigerant,
The through-hole 20 for being connected to cavity 11 Yu atmospheric environment can be offered on 10 wooden partition of heat-conducting plate, it can be experimental
It needs, selects different refrigerants.
Preferably, side wooden partition neighbouring or ipsilateral with 20 place side of through-hole with through-hole 20 on the heat-conducting plate 10 is equipped with
Hook 30.In this way, can be hung on heat-conducting plate 10 in transferring film slot by hook 30, conveniently takes and place.
Stability is hung to improve, the opening of the hook 30 is directed toward ground, and hook 30 is in the left and right sides of heat-conducting plate 10
It is arranged symmetrically.
The heat-conducting plate 10 is made of materials such as the aluminium, copper, alloy of good heat conductivity, for balance service performance and use at
This is, it is preferable to use aluminum alloy materials.
The heat-conducting plate 10 is rectangular-shape, and the plate thickness of heat-conducting plate 10 is 0.05~0.2mm, preferably 0.1mm, will be thermally conductive
Plate 10 is designed to thin-and-long, can obtain small volume, and the big heat-conducting plate 10 of surface area can be filled with transferring film tank liquid
The heat exchange divided is not take up the volume of buffer, favorably to obtain good heat dissipation effect, and 10 small volume of gained heat-conducting plate
In the progress of experiment.
The refrigerant is the liquid that can be absorbed heat after any freezing, including but not limited to isopropyl alcohol and water.
The hole wall of the through-hole 20 is equipped with internal screw thread, and heat-conducting plate 10 further includes screw 40, and screw 40 is equipped with external screw thread
It is adapted with the internal screw thread on 20 hole wall of through-hole.Screw 40 can carry out temporary enclosed processing to through-hole 20, avoid in use process
The volatilization of middle refrigerant and unrestrained.
The present invention is exemplarily described above, it is clear that present invention specific implementation is not subject to the restrictions described above,
As long as using the improvement for the various unsubstantialities that the inventive concept and technical scheme of the present invention carry out, or not improved this is sent out
Bright conception and technical scheme directly apply to other occasions, within the scope of the present invention.Protection of the invention
Range should be determined by the scope of protection defined in the claims.
Claims (8)
1. a kind of WB tests transferring film cooler, it is characterised in that: including heat-conducting plate (10), be equipped with and use in the heat-conducting plate (10)
In the cavity (11) of storage refrigerant.
2. WB according to claim 1 tests transferring film cooler, it is characterised in that: set on heat-conducting plate (10) wooden partition
There is the through-hole (20) for being connected to cavity (11) Yu atmospheric environment.
3. WB according to claim 2 tests transferring film cooler, it is characterised in that: on the heat-conducting plate (10) and through-hole
(20) neighbouring or ipsilateral with side where through-hole (20) side wooden partition is equipped with hook (30).
4. WB according to claim 3 tests transferring film cooler, it is characterised in that: the opening of the hook (30) is directed toward
Ground, hook (30) are arranged symmetrically at left and right sides of heat-conducting plate (10).
5. WB according to claim 1 tests transferring film cooler, it is characterised in that: the heat-conducting plate (10) is by thermal conductivity
The materials such as aluminium, copper, alloy that can be good are made.
6. WB according to claim 1 tests transferring film cooler, it is characterised in that: the heat-conducting plate (10) is cuboid
Shape, the plate thickness of heat-conducting plate (10) are 0.05~0.2mm.
7. WB according to claim 1 tests transferring film cooler, it is characterised in that: the refrigerant be isopropanol or
Water.
8. WB according to claim 2 tests transferring film cooler, it is characterised in that: set on the hole wall of the through-hole (20)
There is internal screw thread, heat-conducting plate (10) further includes screw (40), and screw (40) is equipped with the interior spiral shell on external screw thread and through-hole (20) hole wall
Line is adapted.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811159510.8A CN109174226A (en) | 2018-09-30 | 2018-09-30 | WB tests transferring film cooler |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811159510.8A CN109174226A (en) | 2018-09-30 | 2018-09-30 | WB tests transferring film cooler |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109174226A true CN109174226A (en) | 2019-01-11 |
Family
ID=64946937
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811159510.8A Pending CN109174226A (en) | 2018-09-30 | 2018-09-30 | WB tests transferring film cooler |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109174226A (en) |
Citations (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2162629Y (en) * | 1993-05-07 | 1994-04-20 | 福建福日家用电器集团公司 | Semi-conductor refrigeration vaccine box |
| KR20060107660A (en) * | 2005-04-11 | 2006-10-16 | 엘지전자 주식회사 | Refrigerator and cool storage apparatus and pack of the same |
| CN202041534U (en) * | 2011-03-04 | 2011-11-16 | 冯继锋 | Temperature reduction box for western blot transfer |
| CN202903802U (en) * | 2012-11-19 | 2013-04-24 | 佳木斯大学 | Protein membrane transfer electrophoresis tank temperature reduction device |
| CN203771845U (en) * | 2014-04-09 | 2014-08-13 | 中意电器有限公司 | Cold accumulating box structure and refrigerator with same |
| CN205150684U (en) * | 2015-11-12 | 2016-04-13 | 上海爱萨尔生物科技有限公司 | Heparin tube is ice chest for cold -storage |
| CN105628936A (en) * | 2016-03-22 | 2016-06-01 | 苏州捷美电子有限公司 | Western Blot laboratory instrument |
| CN206082596U (en) * | 2016-09-18 | 2017-04-12 | 温州医科大学附属第二医院 | Can vertical ice chest |
| CN106679261A (en) * | 2017-03-01 | 2017-05-17 | 刘子嘉 | Transfer membrane tank cooling device |
| CN207035619U (en) * | 2017-07-17 | 2018-02-23 | 青岛澳柯玛疫苗冷链有限公司 | A kind of passive vaccine storage box with cryoprotection |
| CN207198170U (en) * | 2017-09-20 | 2018-04-06 | 首都医科大学附属北京中医医院 | Albumen membrane-transferring device |
| CN207876271U (en) * | 2018-01-26 | 2018-09-18 | 广州奇辉生物科技有限公司 | A kind of oncogene Hot spots detection kit |
-
2018
- 2018-09-30 CN CN201811159510.8A patent/CN109174226A/en active Pending
Patent Citations (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2162629Y (en) * | 1993-05-07 | 1994-04-20 | 福建福日家用电器集团公司 | Semi-conductor refrigeration vaccine box |
| KR20060107660A (en) * | 2005-04-11 | 2006-10-16 | 엘지전자 주식회사 | Refrigerator and cool storage apparatus and pack of the same |
| CN202041534U (en) * | 2011-03-04 | 2011-11-16 | 冯继锋 | Temperature reduction box for western blot transfer |
| CN202903802U (en) * | 2012-11-19 | 2013-04-24 | 佳木斯大学 | Protein membrane transfer electrophoresis tank temperature reduction device |
| CN203771845U (en) * | 2014-04-09 | 2014-08-13 | 中意电器有限公司 | Cold accumulating box structure and refrigerator with same |
| CN205150684U (en) * | 2015-11-12 | 2016-04-13 | 上海爱萨尔生物科技有限公司 | Heparin tube is ice chest for cold -storage |
| CN105628936A (en) * | 2016-03-22 | 2016-06-01 | 苏州捷美电子有限公司 | Western Blot laboratory instrument |
| CN206082596U (en) * | 2016-09-18 | 2017-04-12 | 温州医科大学附属第二医院 | Can vertical ice chest |
| CN106679261A (en) * | 2017-03-01 | 2017-05-17 | 刘子嘉 | Transfer membrane tank cooling device |
| CN207035619U (en) * | 2017-07-17 | 2018-02-23 | 青岛澳柯玛疫苗冷链有限公司 | A kind of passive vaccine storage box with cryoprotection |
| CN207198170U (en) * | 2017-09-20 | 2018-04-06 | 首都医科大学附属北京中医医院 | Albumen membrane-transferring device |
| CN207876271U (en) * | 2018-01-26 | 2018-09-18 | 广州奇辉生物科技有限公司 | A kind of oncogene Hot spots detection kit |
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