CN210269867U - Protein immunoblotting transfer membrane box with refrigeration unit - Google Patents
Protein immunoblotting transfer membrane box with refrigeration unit Download PDFInfo
- Publication number
- CN210269867U CN210269867U CN201921255220.3U CN201921255220U CN210269867U CN 210269867 U CN210269867 U CN 210269867U CN 201921255220 U CN201921255220 U CN 201921255220U CN 210269867 U CN210269867 U CN 210269867U
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- film box
- rotary film
- rotary
- refrigeration unit
- box body
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Abstract
The utility model relates to a protein immunoblotting membrane cassette with refrigeration unit belongs to protein immunoblotting test equipment field. The rotary film box comprises a rotary film box body and a rotary film box cover body, wherein the rotary film box body is used for containing a cooling medium, and the rotary film box cover body is detachably connected to the rotary film box body in a sealing manner; the rotating diaphragm capsule body is internally provided with an electrophoresis plate, the electrophoresis plate is provided with an electrode, the rotating diaphragm capsule cover body is provided with an electrode hole for the electrode to pass through, and the rotating diaphragm capsule body is internally provided with a refrigeration unit for reducing the temperature of a cooling medium. The utility model is provided with a refrigeration unit, which can absorb the heat generated in the film transferring process, reduce the temperature in the film transferring process and improve the film transferring efficiency; the refrigeration unit is a semiconductor refrigerator, a container for containing an ice-water mixture is not required to be additionally arranged, the equipment is compact, the occupied space is small, and the operation is convenient; the cooling fan is arranged to cool the hot end of the semiconductor refrigerator, so that the temperature difference between the cold end and the hot end of the semiconductor refrigerator is reduced, and the refrigeration efficiency is improved.
Description
Technical Field
The utility model belongs to western blotting test equipment field relates to a western blotting commentaries on classics membrane box with refrigeration unit.
Background
Western immunoblotting (WB) is a common experimental method for detecting protein expression level, and an important step is membrane transfer, such as dry transfer, semi-dry transfer and wet transfer. The wet-transfer method is the most common method and has the highest efficiency. In the wet-spinning process, a large amount of heat can be generated by the film-spinning instrument due to the action of current in the working process, and the film-spinning efficiency can be reduced due to overhigh temperature. Therefore, it is common practice to fill an ice box in the electrophoresis cassette with ice, then place the electrophoresis cassette in a plastic basin or a foam cassette, and start to rotate the membrane after applying an ice-water mixture to the periphery of the electrophoresis cassette. In the experiment process, ice blocks need to be prepared, the ice adding and preparation processes are complicated, and in the experiment, the cooling water easily wets the ground, so that the experiment environment is clean and tidy.
SUMMERY OF THE UTILITY MODEL
In view of this, the utility model aims at providing an immunity trace of protein commentaries on classics membrane box with refrigerating unit can refrigerate at the commentaries on classics membrane in-process, guarantees to change membrane efficiency.
In order to achieve the above purpose, the utility model provides a following technical scheme: a protein immunoblotting membrane cassette with a refrigeration unit comprises a membrane cassette body for containing a cooling medium and a membrane cassette cover body, wherein the membrane cassette cover body is detachably connected to the membrane cassette body in a sealing manner; the rotary diaphragm box is characterized in that an electrophoresis plate is arranged in the rotary diaphragm box body, an electrode is arranged on the electrophoresis plate, an electrode hole for the electrode to pass through is formed in the rotary diaphragm box cover body, and a refrigeration unit for reducing the temperature of a cooling medium is further arranged in the rotary diaphragm box body.
Optionally, the refrigeration unit is a semiconductor refrigerator.
Optionally, the semiconductor refrigerator is arranged on the rotary diaphragm box cover body, the hot end of the semiconductor refrigerator is exposed on one side of the rotary diaphragm box cover body far away from the rotary diaphragm box body, and the cold end of the semiconductor refrigerator is located in the rotary diaphragm box body.
Optionally, a cooling fan is further disposed on one side of the rotary film box cover body away from the rotary film box body, and the hot end of the semiconductor refrigerator is located in an air flow generated by the cooling fan.
Optionally, the number of the semiconductor refrigerators is two, and the two semiconductor refrigerators are respectively arranged on two sides of the electrophoresis plate.
Optionally, a heat insulation layer is further disposed on the outer side of the rotary diaphragm box body, and the heat insulation layer surrounds the rotary diaphragm box body.
Optionally, change the diaphragm capsule body with be equipped with the connector link between the commentaries on classics diaphragm capsule lid, change the diaphragm capsule lid and pass through the connector link can be dismantled the connection and be in change on the diaphragm capsule body, change the diaphragm capsule lid with still be equipped with the sealing washer between the commentaries on classics diaphragm capsule body.
Optionally, the electrophoresis plate, the refrigeration unit and the cooling fan all adopt external power supplies.
The beneficial effects of the utility model reside in that:
the utility model is provided with a refrigeration unit, which can absorb the heat generated in the film transferring process, reduce the temperature in the film transferring process and improve the film transferring efficiency; the refrigeration unit is a semiconductor refrigerator, a container for containing an ice-water mixture is not required to be additionally arranged, the equipment is compact, the occupied space is small, and the operation is convenient; the cooling fan is arranged to cool the hot end of the semiconductor refrigerator, so that the temperature difference between the cold end and the hot end of the semiconductor refrigerator is reduced, and the refrigeration efficiency is improved.
Additional advantages, objects, and features of the invention will be set forth in part in the description which follows and in part will become apparent to those having ordinary skill in the art upon examination of the following or may be learned from practice of the invention. The objectives and other advantages of the invention may be realized and attained by the means of the instrumentalities and/or combinations particularly pointed out in the appended claims.
Drawings
For the purposes of promoting a better understanding of the objects, features and advantages of the invention, reference will now be made to the following detailed description taken in conjunction with the accompanying drawings in which:
FIG. 1 is a schematic diagram of a protein immunoblotting cassette with a refrigeration unit;
FIG. 2 is a schematic cross-sectional view of a protein immunoblotting cassette having a refrigeration unit;
FIG. 3 is a top view of a protein immunoblotting cassette with a refrigeration unit;
FIG. 4 is a side view of a protein immunoblotting cassette with a refrigeration unit.
Reference numerals: the electrophoresis apparatus comprises a rotary film box body 1, a rotary film box cover body 2, an electrophoresis plate 3, an electrode 4, a semiconductor refrigerator 5, a cooling fan 6 and a connecting buckle 7.
Detailed Description
The following description of the embodiments of the present invention is provided for illustrative purposes, and other advantages and effects of the present invention will be readily apparent to those skilled in the art from the disclosure herein. The present invention can also be implemented or applied through other different specific embodiments, and various details in the present specification can be modified or changed based on different viewpoints and applications without departing from the spirit of the present invention. It should be noted that the drawings provided in the following embodiments are only for illustrating the basic idea of the present invention, and the features in the following embodiments and examples may be combined with each other without conflict.
Wherein the showings are for the purpose of illustrating the invention only and not for the purpose of limiting the same, and in which there is shown by way of illustration only and not in any way limiting the scope of the invention; for a better understanding of the embodiments of the present invention, some parts of the drawings may be omitted, enlarged or reduced, and do not represent the size of an actual product; it will be understood by those skilled in the art that certain well-known structures in the drawings and descriptions thereof may be omitted.
The same or similar reference numerals in the drawings of the embodiments of the present invention correspond to the same or similar parts; in the description of the present invention, it should be understood that if there are terms such as "upper", "lower", "left", "right", "front", "back", etc., indicating directions or positional relationships based on the directions or positional relationships shown in the drawings, it is only for convenience of description and simplification of description, but it is not intended to indicate or imply that the device or element referred to must have a specific direction, be constructed and operated in a specific direction, and therefore, the terms describing the positional relationships in the drawings are only used for illustrative purposes and are not to be construed as limiting the present invention, and those skilled in the art can understand the specific meanings of the terms according to specific situations.
Referring to fig. 1 to 4, a protein immunoblotting rotary film cassette with a refrigeration unit includes a rotary film cassette body 1 for containing a cooling medium and a rotary film cassette cover body 2, wherein the rotary film cassette cover body 2 is detachably and hermetically connected to the rotary film cassette body 1; be equipped with electrophoresis board 3 in changeing diaphragm capsule body 1, be equipped with electrode 4 on the electrophoresis board 3, be equipped with the electrode hole that is used for electrode 4 to pass through on changeing the diaphragm capsule lid 2 body, still be equipped with the refrigeration unit that is used for reducing the coolant temperature in changeing diaphragm capsule body 1.
In this embodiment, the refrigeration unit is a semiconductor refrigerator 5. The semiconductor refrigerator 5 is arranged on the rotary film box cover body 2, the hot end of the semiconductor refrigerator 5 is exposed out of one side of the rotary film box cover body 2 far away from the rotary film box body 1, and the cold end of the semiconductor refrigerator 5 is positioned in the rotary film box body 1. A cooling fan 6 is further arranged on one side of the rotary film box cover body 2 far away from the rotary film box body 1, and the hot end of the semiconductor refrigerator is positioned in air flow generated by the cooling fan 6. The number of the semiconductor refrigerators 5 is two, and the two semiconductor refrigerators 5 are respectively arranged at two sides of the electrophoresis plate 3. The number of the cooling fans 6 is also two, and the two semiconductor refrigerators 5 are respectively positioned in the air flows generated by the two cooling fans 6. The outer side of the rotary film box body 1 is also provided with a heat insulation layer (not shown), and the heat insulation layer surrounds the rotary film box body. Be equipped with connector link 7 between commentaries on classics diaphragm capsule body 1 and the commentaries on classics diaphragm capsule lid, change diaphragm capsule lid 2 and can dismantle the connection on commentaries on classics diaphragm capsule body 1 through connector link 7, in this embodiment, connector link 7 is 4, and 4 connector links 7 revolute the hookup location of commentaries on classics diaphragm capsule lid 2 and diaphragm capsule body 1 and evenly set up, still is equipped with the sealing washer (not drawn) between commentaries on classics diaphragm capsule lid 2 and the commentaries on classics diaphragm capsule body 1.
In this embodiment, the electrophoresis plate 3, the refrigeration unit, and the cooling fan 6 all use external power supplies.
When the electrophoresis film box is actually used, the electrophoresis plate 3 is arranged in the rotary film box body 1, then ice-water mixture is added into the rotary film box body 1 to serve as cooling medium, the rotary film box cover body 2 is connected to the rotary film box body 1 through the connecting buckle 7, the cold end of the semiconductor refrigerator 5 is immersed in the cooling medium in the rotary film box body 1, and the hot end of the semiconductor refrigerator 5 is exposed out of the rotary film box.
The power supply is turned on, the electrophoresis plate 3 is used for film transfer and generates heat, the cold end of the semiconductor refrigerator 5 is used for refrigeration, and the absorbed heat is transferred to the hot end outside the film transfer box so as to reduce the temperature in the film transfer box body 1; the cooling fan 6 works, and the generated air flow cools the hot end of the semiconductor refrigerator 5 so as to improve the refrigeration efficiency of the semiconductor refrigerator 5; the heat insulating layer reduces the heat transfer between the rotary film box body 1 and the external part, further prevents the temperature rise of the cooling medium in the rotary film box body 1, and ensures the film-rotating efficiency.
Finally, the above embodiments are only used for illustrating the technical solutions of the present invention and not for limiting, and although the present invention has been described in detail with reference to the preferred embodiments, it should be understood by those skilled in the art that modifications or equivalent substitutions may be made to the technical solutions of the present invention without departing from the spirit and scope of the technical solutions, and all of them should be covered by the scope of the claims of the present invention.
Claims (7)
1. The utility model provides a protein immunoblotting commentaries on classics membrane cassette with refrigerating unit which characterized in that: the rotary film box comprises a rotary film box body and a rotary film box cover body, wherein the rotary film box body is used for containing a cooling medium, and the rotary film box cover body is detachably connected to the rotary film box body in a sealing manner;
the rotary diaphragm box is characterized in that an electrophoresis plate is arranged in the rotary diaphragm box body, an electrode is arranged on the electrophoresis plate, an electrode hole for the electrode to pass through is formed in the rotary diaphragm box cover body, and a refrigeration unit for reducing the temperature of a cooling medium is further arranged in the rotary diaphragm box body.
2. The protein immunoblotting membrane cassette having a refrigeration unit according to claim 1, wherein: the refrigerating unit is a semiconductor refrigerator.
3. The protein immunoblotting membrane cassette having a refrigeration unit according to claim 2, wherein: the semiconductor refrigerator is arranged on the rotary film box cover body, the hot end of the semiconductor refrigerator is exposed out of one side of the rotary film box cover body, which is far away from the rotary film box body, and the cold end of the semiconductor refrigerator is positioned in the rotary film box body.
4. The protein immunoblotting membrane cassette having a refrigeration unit according to claim 3, wherein: and a cooling fan is further arranged on one side of the rotary film box cover body, which is far away from the rotary film box body, and the hot end of the semiconductor refrigerator is positioned in the air flow generated by the cooling fan.
5. The protein immunoblotting membrane cassette having a refrigeration unit according to claim 2, wherein: the number of the semiconductor refrigerators is two, and the two semiconductor refrigerators are respectively arranged on two sides of the electrophoresis plate.
6. The protein immunoblotting membrane cassette having a refrigeration unit according to claim 1, wherein: the outer side of the rotary film box body is also provided with a heat insulation layer which surrounds the rotary film box body.
7. The protein immunoblotting membrane cassette having a refrigeration unit according to claim 1, wherein: the rotary film box body and a connecting buckle is arranged between the rotary film box cover bodies, the rotary film box cover bodies are detachably connected to the rotary film box body through the connecting buckle, and a sealing ring is further arranged between the rotary film box cover bodies.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201921255220.3U CN210269867U (en) | 2019-08-05 | 2019-08-05 | Protein immunoblotting transfer membrane box with refrigeration unit |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201921255220.3U CN210269867U (en) | 2019-08-05 | 2019-08-05 | Protein immunoblotting transfer membrane box with refrigeration unit |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN210269867U true CN210269867U (en) | 2020-04-07 |
Family
ID=70016027
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201921255220.3U Expired - Fee Related CN210269867U (en) | 2019-08-05 | 2019-08-05 | Protein immunoblotting transfer membrane box with refrigeration unit |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN210269867U (en) |
-
2019
- 2019-08-05 CN CN201921255220.3U patent/CN210269867U/en not_active Expired - Fee Related
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20200407 Termination date: 20210805 |
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| CF01 | Termination of patent right due to non-payment of annual fee |