CN109172659A - A kind of composition and its preparation method and application containing dark plum - Google Patents

A kind of composition and its preparation method and application containing dark plum Download PDF

Info

Publication number
CN109172659A
CN109172659A CN201810948751.4A CN201810948751A CN109172659A CN 109172659 A CN109172659 A CN 109172659A CN 201810948751 A CN201810948751 A CN 201810948751A CN 109172659 A CN109172659 A CN 109172659A
Authority
CN
China
Prior art keywords
parts
ginseng
dark plum
preparation
weight proportion
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201810948751.4A
Other languages
Chinese (zh)
Inventor
沈涛
吴纯洁
文跃强
李敏
吕光华
徐世军
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Chengdu University of Traditional Chinese Medicine
Original Assignee
Chengdu University of Traditional Chinese Medicine
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Chengdu University of Traditional Chinese Medicine filed Critical Chengdu University of Traditional Chinese Medicine
Publication of CN109172659A publication Critical patent/CN109172659A/en
Pending legal-status Critical Current

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/73—Rosaceae (Rose family), e.g. strawberry, chokeberry, blackberry, pear or firethorn
    • A61K36/736—Prunus, e.g. plum, cherry, peach, apricot or almond
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/105—Plant extracts, their artificial duplicates or their derivatives
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/25—Araliaceae (Ginseng family), e.g. ivy, aralia, schefflera or tetrapanax
    • A61K36/258—Panax (ginseng)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
    • A61K36/481—Astragalus (milkvetch)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/88—Liliopsida (monocotyledons)
    • A61K36/896—Liliaceae (Lily family), e.g. daylily, plantain lily, Hyacinth or narcissus
    • A61K36/8968—Ophiopogon (Lilyturf)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
    • A61P1/14—Prodigestives, e.g. acids, enzymes, appetite stimulants, antidyspeptics, tonics, antiflatulents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P37/00—Drugs for immunological or allergic disorders
    • A61P37/02—Immunomodulators
    • A61P37/04—Immunostimulants
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P39/00—General protective or antinoxious agents
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/30—Extraction of the material
    • A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
    • A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation, decoction
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/30—Extraction of the material
    • A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps

Landscapes

  • Health & Medical Sciences (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Animal Behavior & Ethology (AREA)
  • Mycology (AREA)
  • Botany (AREA)
  • Epidemiology (AREA)
  • Microbiology (AREA)
  • Medical Informatics (AREA)
  • Biotechnology (AREA)
  • Alternative & Traditional Medicine (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Immunology (AREA)
  • Nutrition Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Toxicology (AREA)
  • Medicines Containing Plant Substances (AREA)

Abstract

The present invention provides a kind of food, health food or pharmaceutical compositions, it is that the raw material comprising following weight proportion is prepared: 10~100 parts of dark plum, 3~80 parts of ginseng.Wherein, the present composition also includes Radix Ophiopogonis, Radix Astragali, weight proportion are as follows: 10~100 parts of dark plum, 3~80 parts of ginseng, 3~80 parts of Radix Ophiopogonis, 3~80 parts of Radix Astragali.The present invention also provides the preparation method of the composition and purposes.The present composition using the natural medicinal plant of dual-purpose of drug and food, simplify by present composition medication, at low cost, curative for effect, it has no toxic side effect, is not likely to produce tolerance, it is convenient to take, preparation method is simple, and big technique is suitble to produce, and the benefiting from property of patient of cardiopulmonary syndrome of deficiency of both qi and yin is good.

Description

A kind of composition and its preparation method and application containing dark plum
Technical field
The present invention relates to a kind of composition and its preparation method and application containing dark plum, belongs to drug field.
Background technique
Deficiency of both qi and yin, also known as impairment of both QI and YIN.The deficiency of vital energy and the deficiency of Yin are simultaneously and the pathological change seen.It is common in febrile disease process In, heat injures gas yin in gas point, sweating going not thorough long；Or excess heat consumption hurts body fluid, depletion of fluid involving qi desertion；Or warm heat disease later period and internal injury Miscellaneous diseases, Kidney-Yin loss, sap one's vitality.Also seen in certain chronic metabolics, deeline, such as diabetes, tuberculosis, swell The diseases such as tumor.Symptoms include thirsty, shortness of breath etc..Control suitable nourishing generate fluid yin-nourishing.
Currently, clinically for syndrome of deficiency of both qi and yin patient frequently with prescription complexity, the more oral preparation of medication is carried out Treatment.Drug taste number is generally on the high side after being shared due to such prescription, is difficult effectively to control impurity in industrialized production and contains Amount, and be difficult to keep the stability of preparation process, and then can not be popularized in an all-round way interior on a large scale.Therefore, clinically it is badly in need of Want a kind of flavour of a drug less, and clinical efficacy significantly is used to treat the Chinese medicine composition of syndrome of deficiency of both qi and yin.
Summary of the invention
To solve the above problems, the present invention provides a kind of food, health food or pharmaceutical composition, the present invention is also mentioned The preparation method and purposes of the composition are supplied.
The present invention provides a kind of compositions, it is that the raw material comprising following weight proportion is prepared: dark plum 10~ 100 parts, 3~80 parts of ginseng.
Further, it is prepared by the raw material of following weight proportion: 30~90 parts of dark plum, 10~60 parts of ginseng； Preferably, the weight proportion are as follows: 90 parts of dark plum, 40 parts of ginseng.
Further, the composition also includes Radix Ophiopogonis, weight proportion are as follows: 10~100 parts of dark plum, ginseng 3~80 Part, 3~80 parts of Radix Ophiopogonis.
Further, it is prepared by the raw material of following weight proportion: dark plum 40~90,15~60 parts of ginseng, wheat 25~60 parts of winter；Preferably, the weight proportion are as follows: 90 parts of dark plum, 40 parts of ginseng, 60 parts of Radix Ophiopogonis.
Further, the composition also includes Radix Ophiopogonis, Radix Astragali, weight proportion are as follows: 10~100 parts of dark plum, ginseng 3~80 parts, 3~80 parts of Radix Ophiopogonis, 3~80 parts of Radix Astragali.
Further, the composition is prepared by the raw material of following weight proportion: 30~80 parts of dark plum, ginseng 10~60 parts, 10~60 parts of Radix Ophiopogonis, 3~80 parts of Radix Astragali.
Further, it is active constituent that it, which is by the primary powder or water or extractive with organic solvent of the raw material, is added Enter complementary ingredient and is prepared into common preparation；
Wherein, the preparation is oral preparation, injection or powder-injection；
Oral preparation are as follows: decoction, pill, tablet, effervescent tablet, capsule, pulvis, granule, syrup or oral solution；
The auxiliary material be filler, disintegrating agent, lubricant, suspending agent, adhesive, sweetener, corrigent, preservative, The combination of one or more of matrix；
Wherein, filler is starch, in pregelatinized starch, lactose, mannitol, chitin, microcrystalline cellulose, sucrose A combination of one or more；
Disintegrating agent be starch, pregelatinized starch, microcrystalline cellulose, sodium carboxymethyl starch, crosslinked polyvinylpyrrolidone, The combination of one or more of low-substituted hydroxypropyl cellulose, croscarmellose sodium；
Lubricant is one or more of magnesium stearate, lauryl sodium sulfate, talcum powder, silica Combination；
Suspending agent is one of polyvinylpyrrolidone, microcrystalline cellulose, sucrose, agar, hydroxypropyl methyl cellulose Or two or more combination；
Adhesive is the group of one or more of starch slurry, polyvinylpyrrolidone, hydroxypropyl methyl cellulose It closes；
Sweetener is the group of one or more of saccharin sodium, aspartame, sucrose, honey element, enoxolone It closes；
Corrigent is one or both of sweetener or essence；
Preservative is parabens, benzoic acid, sodium benzoate, sorbic acid and its esters, benzalkonium bromide, acetic acid chloroethene are fixed, Eucalyptus oil etc.；Matrix includes: PEG6000, PEG4000, the combination of one or more of insect wax.
The present invention also provides a kind of methods for preparing combinations of the above object, it includes the following steps:
A) bulk pharmaceutical chemicals of weight proportion are weighed；
B) water that 5~8 times of raw material weights are added impregnates 1~2h, decocts twice, every time 1~1.5h, merging filtrate；
C) auxiliary material is added in the filtrate was concentrated to dryness medicinal extract, be prepared into preparation to get.
The present invention also provides combinations of the above objects to have Yiqi and vein recovery, the health food or medicine of nourishing Yin and promoting production of body fluid in preparation Purposes in compositions.
The present invention also provides combinations of the above objects to have the health food or pharmaceutical composition for improving immunity in preparation In purposes.
The present composition using the natural medicinal plant of dual-purpose of drug and food, simplify by present composition medication, cost It is low, it is curative for effect, also have the function of synergy under special ratios, and have no toxic side effect, be not likely to produce tolerance, takes With conveniently, preparation method is simple, and big technique is suitble to produce, and the benefiting from property of patient of cardiopulmonary syndrome of deficiency of both qi and yin is good.
Obviously, above content according to the present invention is not taking off according to the ordinary technical knowledge and customary means of this field Under the premise of the above-mentioned basic fundamental thought of the present invention, the modification, replacement or change of other diversified forms can also be made.
The specific embodiment of form by the following examples remakes above content of the invention further detailed Explanation.But the range that this should not be interpreted as to the above-mentioned theme of the present invention is only limitted to example below.It is all above-mentioned interior based on the present invention Hold realized technology to all belong to the scope of the present invention.
Specific embodiment
The preparation of embodiment 1, the present composition
1. raw material: dark plum 60g, ginseng 60g.
2. preparation method:
A) raw material of weight proportion is weighed；
B) water for measuring all raw materials with 5~8 times impregnates 1~2 hour, decocts 1~1.5 hour, obtains after the completion of impregnating 1st decocting liquid, the dregs of a decoction retain；
C) dregs of a decoction in step b are taken, the water that 5~8 times of raw material amounts are added decocts 1~1.5 hour, filters after the completion of decocting The 2nd decocting liquid is obtained, the dregs of a decoction are discarded；
D) merging filtrate to get.
The preparation of embodiment 2, the present composition
1. raw material: dark plum 60g, ginseng 54g.
2. preparation method:
A) raw material of weight proportion is weighed；
B) water for measuring all raw materials with 5~8 times impregnates 1~2 hour, decocts 1~1.5 hour, obtains after the completion of impregnating 1st decocting liquid, the dregs of a decoction retain；
C) dregs of a decoction in step b are taken, the water that 5~8 times of raw material amounts are added decocts 1~1.5 hour, filters after the completion of decocting The 2nd decocting liquid is obtained, the dregs of a decoction are discarded；
D) merging filtrate is condensed into medicinal extract, adds pharmaceutically common auxiliary material, is prepared into common oral preparation.
The preparation of embodiment 3, the present composition
1. raw material: dark plum 45g, ginseng 12g.
2. preparation method:
A) raw material of weight proportion is weighed；
B) water for measuring all raw materials with 5~8 times impregnates 1~2 hour, decocts 1~1.5 hour, obtains after the completion of impregnating 1st decocting liquid, the dregs of a decoction retain；
C) dregs of a decoction in step b are taken, the water that 5~8 times of raw material amounts are added decocts 1~1.5 hour, filters after the completion of decocting The 2nd decocting liquid is obtained, the dregs of a decoction are discarded；
D) merging filtrate is condensed into medicinal extract, adds pharmaceutically common auxiliary material, is prepared into common injection.
The preparation of embodiment 4, the present composition
1. raw material: dark plum 90g, ginseng 40g.
2. preparation method: with embodiment 1.
The preparation of embodiment 5, the present composition
1. raw material: dark plum 30g, ginseng 10g.
2. preparation method: with embodiment 1.
The preparation of embodiment 6, the present composition
1. raw material: dark plum 60g, ginseng 10g.
2. preparation method: with embodiment 1.
The preparation of embodiment 7, the present composition
1. raw material: dark plum 90g, ginseng 10g.
2. preparation method: with embodiment 1.
The preparation of embodiment 8, the present composition
1. raw material: dark plum 90g, ginseng 60g.
2. preparation method: with embodiment 1.
The preparation of embodiment 9, the present composition
1. raw material: dark plum 60g, ginseng 60g, Radix Ophiopogonis 60g.
2. preparation method:
A) raw material of weight proportion is weighed；
B) water for measuring all raw materials with 5~8 times impregnates 1~2 hour, decocts 1~1.5 hour, obtains after the completion of impregnating 1st decocting liquid, the dregs of a decoction retain；
C) dregs of a decoction in step b are taken, the water that 5~8 times of raw material amounts are added decocts 1~1.5 hour, filters after the completion of decocting The 2nd decocting liquid is obtained, the dregs of a decoction are discarded；
D) merging filtrate to get.
The preparation of embodiment 10, the present composition
1. raw material: dark plum 40g, ginseng 15g, Radix Ophiopogonis 25g.
2. preparation method:
A) raw material of weight proportion is weighed；
B) water for measuring all raw materials with 5~8 times impregnates 1~2 hour, decocts 1~1.5 hour, obtains after the completion of impregnating 1st decocting liquid, the dregs of a decoction retain；
C) dregs of a decoction in step b are taken, the water that 5~8 times of raw material amounts are added decocts 1~1.5 hour, filters after the completion of decocting The 2nd decocting liquid is obtained, the dregs of a decoction are discarded；
D) merging filtrate is condensed into medicinal extract, and pharmaceutically common auxiliary material is prepared into granule for addition.
The preparation of embodiment 11, the present composition
1. raw material: dark plum 45g, ginseng 20g, Radix Ophiopogonis 45g.
2. preparation method:
A) raw material of weight proportion is weighed；
B) water for measuring all raw materials with 5~8 times impregnates 1~2 hour, decocts 1~1.5 hour, obtains after the completion of impregnating 1st decocting liquid, the dregs of a decoction retain；
C) dregs of a decoction in step b are taken, the water that 5~8 times of raw material amounts are added decocts 1~1.5 hour, filters after the completion of decocting The 2nd decocting liquid is obtained, the dregs of a decoction are discarded；
D) merging filtrate is condensed into medicinal extract, and pharmaceutically common auxiliary material is prepared into tablet for addition.
The preparation of embodiment 12, the present composition
1. raw material: dark plum 90g, ginseng 40g, Radix Ophiopogonis 60g.
2. preparation method:
A) raw material of weight proportion is weighed；
B) water for measuring all raw materials with 5~8 times impregnates 1~2 hour, decocts 1~1.5 hour, obtains after the completion of impregnating 1st decocting liquid, the dregs of a decoction retain；
C) dregs of a decoction in step b are taken, the water that 5~8 times of raw material amounts are added decocts 1~1.5 hour, filters after the completion of decocting The 2nd decocting liquid is obtained, the dregs of a decoction are discarded；
D) merging filtrate is condensed into medicinal extract, and pharmaceutically common auxiliary material is prepared into tablet for addition.
The preparation of embodiment 13, the present composition
1. raw material: dark plum 60g, ginseng 60g, Radix Ophiopogonis 60g, Radix Astragali 60g.
2. preparation method:
A) raw material of weight proportion is weighed；
B) water for measuring all raw materials with 5~8 times impregnates 1~2 hour, decocts 1~1.5 hour, obtains after the completion of impregnating 1st decocting liquid, the dregs of a decoction retain；
C) dregs of a decoction in step b are taken, the water that 5~8 times of raw material amounts are added decocts 1~1.5 hour, filters after the completion of decocting The 2nd decocting liquid is obtained, the dregs of a decoction are discarded；
D) merging filtrate to get.
The preparation of embodiment 14, the present composition
1. raw material: dark plum 52g, ginseng 15g, Radix Ophiopogonis 20g, Radix Astragali 35g.
2. preparation method:
A) raw material of weight proportion is weighed；
B) water for measuring all raw materials with 5~8 times impregnates 1~2 hour, decocts 1~1.5 hour, obtains after the completion of impregnating 1st decocting liquid, the dregs of a decoction retain；
C) dregs of a decoction in step b are taken, the water that 5~8 times of raw material amounts are added decocts 1~1.5 hour, filters after the completion of decocting The 2nd decocting liquid is obtained, the dregs of a decoction are discarded；
D) merging filtrate is condensed into medicinal extract, supplementary product starch 20g granulation is added, magnesium stearate 2g, dextrin 20g, crystallite are fine Tie up element 20g, uniformly obtained particle, tabletting to get.
Beneficial effects of the present invention are verified below by specific pharmacy test:
The influence of test example 1, pharmaceutical composition of the present invention to cyclophosphamide intraperitoneal injection model mice immune function
1. experimental material:
1.1 experimental animals:
100 SPF grades of KM mouse, entirely20 ± 2g of weight, by People's Hospital, Sichuan Prov. of Sichuan Academy of Medical Sciences Institute of lab animals provides, animal credit number: SCXK (river) 2013-15.
1.2 drugs and reagent:
Dark plum, ginseng, Radix Ophiopogonis, Radix Astragali are from Sichuan Naruhito hall medicine company chain co., ltd, injection cyclophosphamide, Hengrui Medicine Co., Ltd., Jiangsu Prov., number: 111817；India ink, Shanghai Ming Bo Biotechnology Co., Ltd.
1.3 instrument and equipments:
FA1204L electronic balance, Shanghai Yue Ping scientific instrument Co., Ltd；WH-3 vortex oscillation instrument, the analysis of Shanghai Luxi Instrument Co., Ltd., Factory；TDZ5-WS centrifuge, Hunan Xiang Yi Laboratory Instruments development corporation, Ltd.；HH.W21-420S constant temperature Water bath, Shanghai leap Medical Devices Co., Ltd.；PQF-60 type research fluorescence microscope, optical instrument of holding high up is composed in Shanghai to be had Limit company.
2. experimental method
2.1 grouping administrations:
5 groups, i.e. blank group, model group, experiment A group will be randomly divided by weight after 100 mouse adaptable fed 3d (example 1 drug, dark plum: ginseng=1:1), experiment B group (9 drug of embodiment, dark plum: ginseng: Radix Ophiopogonis=1:1:1), experiment C group (13 drug of embodiment, dark plum: ginseng: Radix Ophiopogonis: Radix Astragali=1:1:1:1), every group 20.Relative medicine is given in daily stomach-filling 1 time, blank group and model group give isometric distilled water, dosage 3.2g/kg, administered volume 10ml/kg, successive administration 10d.In addition to blank group, the cyclophosphamide that remaining each group is newly prepared in administration 2d intraperitoneal injection 75mg/kg, continuous 3d system Standby immunosuppressed mice model.
2.2 Carbon grain Kuo clear laws measure non-specific immune function:
After 11d last dose 30min, 10 mouse tail vein injection india ink 0.01ml/g (injection is randomly selected It is preceding to use 10 times of normal saline dilution), 15min eye socket takes blood 40ul that 4ml 0.1%NaCO is added3In solution, after mixing well Absorbance is measured at 600nm, with 0.1%NaCO3Solution is blank well, calculates phagocyte imdex and phagocytic index.
The detection of 2.3 Thymus and Spleen indexes:
By 1h after the remaining 10 mouse administration of each group, cervical dislocation is put to death, then clip thymus gland and spleen, and is weighed immediately, Calculate its Thymus and Spleen index.
3. experimental result:
The influence of 3.1 pairs of immunosuppressed mice organ indexs
Influence of the table 1. to immunosuppressed mice organ index
Note: compared with blank group,#P < 0.05,##P<0.01；Compared with model group,*P < 0.05,**P<0.01。
As shown in Table 1, compared with blank group, model group mouse phagocyte imdex, phagocytic index, thymus index and spleen Dirty index is all decreased obviously (P < 0.05), illustrates the i.e. model success of model mice immunologic hypofunction；Compared with model group, respectively Experimental group can improve These parameters in various degree, wherein experiment A, B, C group phagocyte imdex, phagocytic index and thymus gland Index has significant statistical difference (P < 0.05), and experiment A group index and spleen index increases, but without significant difference, tests B, C Group can significantly improve mouse spleen index, and have statistical difference (P < 0.05).Above data shows to test A, B, C group Have the function of improving body's immunity.
The antifatigue experiment of test example 2, the present composition
1. materials and methods
1.1 material
1.1.1 drug
Dark plum, Radix Ophiopogonis, ginseng (life in 3 years) pharmaceutical decocting piece (Sichuan Tuo Jiangyuan pharmaceutcal corporation, Ltd)；Three Le slurry is antifatigue Liquid (the three magnificent pharmaceutical Co. Ltd in Sichuan of Le Jiang medicine company group of Chengdu).
1.1.2 reagent
Blood lactase acid assay kit, hepatic glycogen assay kit, (biology is built up in Nanjing to serum urea nitrogen assay kit Engineering research institute), lot number 20180330；The concentrated sulfuric acid (domestic analysis is pure).
1.1.3 experimental animal
SPF Kunming mice, weight 18-22g, male are tested Company of Animals Ltd. up to rich fruit by Chengdu and are provided, close Lattice card number: SCXK (river) 2015-030, freer drinking-water of ingesting, and control room temperature is 20~25 DEG C, presses after adaptable fed 3d Weight is grouped at random.
1.1.4 instrument
FA1104 type analysis electronic balance (upper current chart level instruments and meters Co., Ltd)；The multi-functional reading of full wavelength scanner formula Number instrument (silent winged scientific and technological (China) Co., Ltd of generation that of match)；Z32HK type centrifuge (the limited public affairs of He Mo (Shanghai) instrument science and technology Department), swimming trunk.
1.2 method
1.2.1 it is grouped and is administered setting
Totally 13 groups of mouse, every group 10, blank group, three Le slurry groups and 11 different proportion groups are randomly divided into, test A group (dark plum: ginseng=1:1), experiment B group (dark plum: ginseng: Radix Ophiopogonis=1:1:1), experiment C group (dark plum: ginseng: Radix Ophiopogonis: Radix Astragali =1:1:1:1), experiment D (dark plum: ginseng=3:1), experiment E (dark plum: ginseng=6:1), experiment F (dark plum: ginseng=9: 1) G (dark plum: ginseng=9:4), experiment H (dark plum: ginseng=9:6), experiment I (dark plum: ginseng=1:3), experiment J, are tested (dark plum: ginseng=1:6), experiment K (dark plum: ginseng=1:9), (is equivalent to 60kg adult and takes 12g) according to 2g/kg dosage Carry out stomach-filling；Administered volume 10ml/kg, continuous gavage 7d.
1.2.2 mouse swimming test and blood lactase acid measurement
After last dose 30min, in the lead of 10% weight of mouse tail root weight bearing, in swimming trunk, (depth of water is not less than Carry out swimming test in 30cm), 25 ± 1.0 DEG C of water temperature, record mouse exhausts to power that (10s that sinks under the water cannot be floated from swim The water surface out) time.3 adaptability swimming instructions are carried out before each swimming test, take not load mode, 1 times/day, 1 time 5min, the individual difference caused by reducing because of radical response are excessive.After mice burden swimming test, mouse is dried and cut off Beard carries out pulling out immediately eyeball and takes blood, separates serum, by kit method measurement blood lactase acid amount.
1.2.3 hepatic glycogen measures
After last dose 30min, mouse carries out not swimming with a load attached to the body 90min under conditions of water temperature is 30 DEG C, vertical after having swum It puts to death and takes fresh liver, rinsed through physiological saline and blotted with filter paper, measure hepatic glycogen amount by kit method.
1.2.4 determination of urea nitrogen
After last dose 30min, mouse carries out not swimming with a load attached to the body 90min in the swimming trunk that water temperature is 30 DEG C, rests Then beard is dried and cut off to mouse by 60min, carry out pulling out eyeball taking blood immediately, and separate serum, surveys by kit method Determine serum urea nitrogen amount.
1.2.5 statistical analysis experimental data establishes database using Excel.It is carried out at data with 23.0 software of SPSS Reason.Data are usedIt indicates, P < 0.05 is statistically significant.
2. result
Influence of the 2.1 each ratio compounds to mouse swimming time
Influence of each ratio compound of table 2 to the mice burden swimming time
Note: compared with the control group, P < 0.01 * P < 0.05, * *.
From table 2 it can be seen that each experimental group can extend the weight carrying swimming of mouse, wherein experiment D, experiment E, experiment F, Significant difference (P < 0.05) compared with blank control group H is tested, three Le slurry groups, experiment G group difference compared with blank control group are aobvious It writes (P < 0.01).
2.2 hepatic glycogen measurement results
The testing result of each experimental group hepatic glycogen, urea nitrogen, blood lactase acid this three biochemical indicators, is shown in Table 3.
Influence of each experimental group of table 3 to mouse swimming time, hepatic glycogen, serum urea nitrogen, blood lactase acid
Note: three Le starch anti-fatigue liquid specification: 0.5g crude drug/ml, adult dosage are as follows: 30ml/ branch, 1/day.With blank group Compare, P < 0.05 * * P < 0.01, *.
As shown in Table 3, compared to the blank group, each test dose group can significantly extend mouse swimming time, improve glycogen Former amount reduces urea nitrogen, blood lactase acid amount (P < 0.01 or P < 0.05), wherein experiment G (dark plum: ginseng=9:4) agent The effect of amount group is optimal.According to the requirement of the Ministry of Public Health's " health food is examined and assessment technique specification ", the judgement side of antifatigue effect Method: swimming with a load attached to the body experimental result is positive, and 3 binomials in three blood lactase acid, serum urea, hepatic glycogen/muscle glycogen biochemical indicators Index is positive, that is, can determine that the tested material has the function of alleviating physical fatigue.
Test example 3, the influence to cyclophosphamide intraperitoneal injection model mice immune function
1. experimental material:
1.1 experimental animals:
70 SPF grades of KM mouse, entirely20 ± 2g of weight, by People's Hospital, Sichuan Prov. of Sichuan Academy of Medical Sciences reality Institute of Botany offer, animal credit number: SCXK (river) 2013-15 are provided.
1.2 drugs and reagent:
Dark plum, ginseng, Radix Ophiopogonis, Radix Astragali are from Sichuan Naruhito hall medicine company chain co., ltd, injection cyclophosphamide, Hengrui Medicine Co., Ltd., Jiangsu Prov., number: 111817；India ink, Shanghai Ming Bo Biotechnology Co., Ltd.
1.3 instrument and equipments:
FA1204L electronic balance, Shanghai Yue Ping scientific instrument Co., Ltd；WH-3 vortex oscillation instrument, the analysis of Shanghai Luxi Instrument Co., Ltd., Factory；TDZ5-WS centrifuge, Hunan Xiang Yi Laboratory Instruments development corporation, Ltd.；HH.W21-420S constant temperature Water bath, Shanghai leap Medical Devices Co., Ltd.；PQF-60 type research fluorescence microscope, optical instrument of holding high up is composed in Shanghai to be had Limit company.
2. experimental method
2.1 grouping administrations:
7 groups will be randomly divided by weight after 70 mouse adaptable fed 3d, i.e. blank group, model group, experiment A group is (real Apply 1 drug of example, dark plum: ginseng=1:1), experiment B group (9 drug of embodiment, dark plum: ginseng: Radix Ophiopogonis=1:1:1), experiment C group (13 drug of embodiment, dark plum: ginseng: Radix Ophiopogonis: Radix Astragali=1:1:1:1), experiment D (dark plum: ginseng=9:4), experiment E (crow Plum: ginseng: Radix Ophiopogonis=9:4:6).Every group 10.Daily stomach-filling is given relative medicine 1 time, and blank group and model group, which are given, waits bodies Product distilled water, dosage 3.2g/kg, administered volume 10ml/kg, successive administration 10d.In addition to blank group, remaining each group is equal In the cyclophosphamide that administration 2d intraperitoneal injection 75mg/kg is newly prepared, continuous 3d prepares immunosuppressed mice model.
2.2 Carbon grain Kuo clear laws measure non-specific immune function:
With test example 1.
The detection of 2.3 Thymus and Spleen indexes:
With test example 1.
3. experimental result:
The influence of 3.1 pairs of immunosuppressed mice organ indexs
Influence of the table 4 to immunosuppressed mice organ index
Note: compared with blank group,#P < 0.05,##P<0.01；Compared with model group,*P < 0.05,**P<0.01。
As shown in Table 4, compared with blank group, model group mouse phagocyte imdex, phagocytic index, thymus index and spleen Dirty index is all decreased obviously (P < 0.05), illustrates the i.e. model success of model mice immunologic hypofunction；Compared with model group, respectively Experimental group can improve These parameters in various degree, show that experiment D group experiment E group effect is the most obvious, have and improve body The effect of immune function.
Shown under the premise of identical dosage by above-mentioned experiment, curative effect can be obtained more by various dose proportion For significant pharmaceutical composition, show that dark plum, ginseng, Radix Ophiopogonis have the function of synergy by above-mentioned dose ratio compatibility.
To sum up, natural medicinal plant of the present composition using dual-purpose of drug and food, present composition medication essence Letter, it is at low cost, it is curative for effect, also have the function of synergy under special ratios, and have no toxic side effect, is not likely to produce resistance to By property, convenient to take, preparation method is simple, and big technique is suitble to produce, and the benefiting from property of patient of cardiopulmonary syndrome of deficiency of both qi and yin is good.

Claims (10)

1. a kind of food, health food, pharmaceutical composition, it is characterised in that: it is prepared by the raw material comprising following weight proportion It forms: 10~100 parts of dark plum, 3~80 parts of ginseng.
2. composition according to claim 1, it is characterised in that: it is prepared by the raw material of following weight proportion: 30~90 parts of dark plum, 10~60 parts of ginseng；Preferably, the weight proportion are as follows: 90 parts of dark plum, 40 parts of ginseng.
3. composition according to claim 1, it is characterised in that: the composition also includes Radix Ophiopogonis, weight proportion Are as follows: 10~100 parts of dark plum, 3~80 parts of ginseng, 3~80 parts of Radix Ophiopogonis.
4. composition according to claim 3, it is characterised in that: it is prepared by the raw material of following weight proportion: Dark plum 40~90,15~60 parts of ginseng, 25~60 parts of Radix Ophiopogonis；Preferably, the weight proportion are as follows: 90 parts of dark plum, 40 parts of ginseng, 60 parts of Radix Ophiopogonis.
5. composition according to claim 1, it is characterised in that: the composition also includes Radix Ophiopogonis, Radix Astragali, weight Proportion are as follows: 10~100 parts of dark plum, 3~80 parts of ginseng, 3~80 parts of Radix Ophiopogonis, 3~80 parts of Radix Astragali.
6. composition according to claim 5: it is characterized by: raw material system of the composition by following weight proportion It is standby to form: 30~80 parts of dark plum, 10~60 parts of ginseng, 10~60 parts of Radix Ophiopogonis, 3~80 parts of Radix Astragali.
7. composition described in -6 any one according to claim 1, it is characterised in that: it is the primary powder by the raw material End or water or extractive with organic solvent are active constituent, and complementary ingredient is added and is prepared into common preparation；
Wherein, the preparation is oral preparation, injection or powder-injection；
Oral preparation are as follows: decoction, pill, tablet, effervescent tablet, capsule, pulvis, granule, syrup or oral solution；
The auxiliary material is filler, disintegrating agent, lubricant, suspending agent, adhesive, sweetener, corrigent, preservative, matrix One or more of combination；
Wherein, filler be one of starch, pregelatinized starch, lactose, mannitol, chitin, microcrystalline cellulose, sucrose or Two or more combinations；
Disintegrating agent is starch, pregelatinized starch, microcrystalline cellulose, sodium carboxymethyl starch, crosslinked polyvinylpyrrolidone, low substitution The combination of one or more of hydroxypropylcellulose, croscarmellose sodium；
Lubricant is the combination of one or more of magnesium stearate, lauryl sodium sulfate, talcum powder, silica；
Suspending agent is one of polyvinylpyrrolidone, microcrystalline cellulose, sucrose, agar, hydroxypropyl methyl cellulose or two Kind or more combination；
Adhesive is the combination of one or more of starch slurry, polyvinylpyrrolidone, hydroxypropyl methyl cellulose；
Sweetener is the combination of one or more of saccharin sodium, aspartame, sucrose, honey element, enoxolone；
Corrigent is one or both of sweetener or essence；
Preservative is parabens, benzoic acid, sodium benzoate, sorbic acid and its esters, benzalkonium bromide, acetic acid chloroethene are fixed, folium eucalypti Oil etc.；Matrix includes: PEG6000, PEG4000, the combination of one or more of insect wax.
8. a kind of method for preparing composition described in claim 1-7 any one, it is characterised in that: it includes following step It is rapid:
A) bulk pharmaceutical chemicals of weight proportion are weighed；
B) water that 5~8 times of raw material weights are added impregnates 1~2h, decocts twice, every time 1~1.5h, merging filtrate；
C) auxiliary material is added in the filtrate was concentrated to dryness medicinal extract, be prepared into preparation to get.
9. composition described in claim 1-7 any one preparation have Yiqi and vein recovery, the health food of nourishing Yin and promoting production of body fluid or Purposes in pharmaceutical composition.
10. composition described in claim 1-7 any one has the health food or medicine group for improving immunity in preparation Close the purposes in object.
CN201810948751.4A 2017-09-06 2018-08-20 A kind of composition and its preparation method and application containing dark plum Pending CN109172659A (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CN201710797186 2017-09-06
CN2017107971861 2017-09-06

Publications (1)

Publication Number Publication Date
CN109172659A true CN109172659A (en) 2019-01-11

Family

ID=64919052

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201810948751.4A Pending CN109172659A (en) 2017-09-06 2018-08-20 A kind of composition and its preparation method and application containing dark plum

Country Status (1)

Country Link
CN (1) CN109172659A (en)

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1977884A (en) * 2005-12-08 2007-06-13 天津中新药业集团股份有限公司乐仁堂制药厂 Medicinal composition for treating diabetes and its preparing method
CN101095548A (en) * 2007-07-19 2008-01-02 丁伟强 Body-strengthening health-care beverage

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1977884A (en) * 2005-12-08 2007-06-13 天津中新药业集团股份有限公司乐仁堂制药厂 Medicinal composition for treating diabetes and its preparing method
CN101095548A (en) * 2007-07-19 2008-01-02 丁伟强 Body-strengthening health-care beverage

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
曹荣: "《小茶包 大健康》", 30 June 2015, 中国医药科技出版社 *

Similar Documents

Publication Publication Date Title
CN104873624B (en) A kind of pharmaceutical composition for gouty arthritis
CN101310751A (en) Traditional Chinese medicine composition for replenishing qi and blood, preparation method and quality control method thereof
CN102349975A (en) Health care product formula of dendrobium officinale, composition as well as preparation method and application of composition
CN100400075C (en) Qi-invigorating, blood-nourishing medicinal composition and its preparing method
CN104667044A (en) Traditional Chinese medicine preparation for treating arthralgia and preparation method of traditional Chinese medicine preparation
CN104940479A (en) TCM composition for treating AD diseases
CN104857435B (en) A kind of Traditional Chinese medicine compound composition with antitumor activity and its preparation method and application
CN102145089B (en) Traditional Chinese medicine for treating heart failure
CN103520318B (en) Traditional Chinese medicine composition of enhancing immune function and preparation method of composition
CN101317964A (en) Chinese medicinal composition with spleen-enlivening and kidney-tonifying function, preparation and uses thereof
CN101732566B (en) Traditional Chinese medicine compound preparation for tonifying liver and kidney and strengthening bones and muscles
CN103735621B (en) A kind of Chinese medicine composition with blood fat reducing and enhancing immunity effect
CN105434738A (en) Traditional Chinese medicine composition for treating ADHD (Attention Deficit Hyperactivity Disorder) and preparation method thereof
CN1927324A (en) Preparation method of traditional medicine preparation for treating women&#39;s menoxenia
CN102488838A (en) Traditional Chinese medicine composition used for treating viral myocarditis
CN102784230B (en) Pharmaceutical composition preparation for treating nutritional anemia
CN104042895A (en) Traditional Chinese medicine composition for treating systemic lupus erythematosus and use thereof
CN109172659A (en) A kind of composition and its preparation method and application containing dark plum
CN102652774A (en) Drug composition for treating leukopenia and hypoimmunity caused by chemoradiotherapy and preparation method and quality detection method
CN1434042A (en) Cattail pollen extract and preparation process and use thereof
CN102178759A (en) Traditional Chinese medicine composition for treating rheumatism and preparation method of composition
JP2023505620A (en) Chinese herbal composition and its production method and use
CN103830584B (en) Medicine for the treatment of Vertebral Artery Ischemic Disease and preparation method thereof
CN105727089A (en) Application of medicine composition containing folium artemisiae argyi to preparing medicine for treating irritable bowel syndrome
CN104906194B (en) A kind of medicinal plaster treating asthma and preparation technology and determination method

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination