CN109164044A - A kind of slice automatic scanning system and method - Google Patents
A kind of slice automatic scanning system and method Download PDFInfo
- Publication number
- CN109164044A CN109164044A CN201810960647.7A CN201810960647A CN109164044A CN 109164044 A CN109164044 A CN 109164044A CN 201810960647 A CN201810960647 A CN 201810960647A CN 109164044 A CN109164044 A CN 109164044A
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- China
- Prior art keywords
- slice
- automatic
- microscope
- scanning system
- cabinet
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Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2201/00—Features of devices classified in G01N21/00
- G01N2201/04—Batch operation; multisample devices
- G01N2201/0492—Automatised microscope
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2201/00—Features of devices classified in G01N21/00
- G01N2201/10—Scanning
- G01N2201/102—Video camera
Abstract
The invention discloses a kind of slice automatic scanning system and method, it solves the problems, such as sections observation exists in the prior art, and time-consuming, precision is not high, has the effect of the automatic access, the automatically scanning that can be realized slice；Its technical solution are as follows: including automatic feeding device and slice automatic sweep apparatus, automatic feeding device includes the slice storehouse for accommodating slice cabinet, is sliced inside storehouse and is equipped with the fetching device that can be rotated, go up and down, stretching；Fetching device in-built electrical magnet, slice when electromagnet is powered in clamping slice cabinet；Slice automatic sweep apparatus receives the slice that takes out through fetching device and fixation, including microscope and is installed on the automatic liquid-dropping mechanism of microscope side；The microscope is configured like equipment, and imaging device shoots amplified sectioning image, and is sent to scanning control system.
Description
Technical field
The present invention relates to slice scanning technique field more particularly to a kind of slice automatic scanning system and methods.
Background technique
Traditional slice detection is usually that slice is placed on microscopical objective table, is selected by manual operation different
Amplification factor, to search for target.This mode requires operator to have a degree of professional knowledge, and can only once observe one
A regional area, for needing to observe the slice of multizone, then short time consumption is long.Moreover, the precision of traditional view mode is not
It is high.
Summary of the invention
For overcome the deficiencies in the prior art, the present invention provides a kind of slice automatic scanning system and method, tools
There is the effect of the automatic access, automatically scanning that can be realized slice.
The present invention adopts the following technical solutions:
A kind of slice automatic scanning system, comprising:
Automatic feeding device, including the slice storehouse for accommodating slice cabinet, being sliced to be equipped with inside storehouse can rotate, rise
Drop, flexible fetching device；Fetching device in-built electrical magnet, slice when electromagnet is powered in clamping slice cabinet；
It is sliced automatic sweep apparatus, receives the slice that takes out through fetching device and fixation, including microscope and is installed on aobvious
The automatic liquid-dropping mechanism of micro mirror side；The microscope is configured like equipment, and imaging device shoots amplified sectioning image, and
It is sent to scanning control system.
Further, the fetching device is connected by telescopic device with lifting device, and the lifting device is installed on rotation
Above rotary device.
Further, the fetching device includes supporting plate, and the bottom of the support plate is fixed with the upper clip of interval set distance under
The bottom of intermediate plate, upper clip is fixed with electromagnet.
Further, the rotating device includes turntable and the inside and outside swiveling gear mechanism for driving turntable.
Further, the lifting device uses guiding rail mechanism, and telescopic device uses screw mechanism.
Further, the inner wall in the slice storehouse circumferentially opens up several for placing the groove of slice cabinet, is sliced storehouse one
Side is provided with outlet.
Further, there is multilayer wall reserve tank, slice cabinet top portion is equipped with extraction tank inside the slice cabinet.
Further, the automatic liquid-dropping mechanism includes dispensing container, dropping liquid conduit and peristaltic pump, and the peristaltic pump passes through
Dropping liquid conduit is connected with dispensing container, and the angle of operation by controlling peristaltic pump realizes the control to dropping liquid.
Further, the imaging device is industrial camera, and industrial camera passes through Microscope Adapter and microscopical one
A eyepiece is connected.
A kind of control method being sliced automatic scanning system, comprising the following steps:
The mobile microscopical objective table of step (1), receives the slice through automatic feeding device outlet output；
Slice is moved to below object lens mouth, low power objective is switched to working condition by step (2) moving stage；
Step (3) adjusts microscope Z axis, shoots sectioning image by industrial camera, and judge whether to focus；
Step (4) realizes slice scanning by moving stage to defocused, and records the scanning in slice specific modality region
Point coordinate；Step (3) are repeated when not focusing；
After step (5) is sliced the low power objective end of scan, high power objective is switched into working condition, and execute step (3)；
Scanning element coordinate of the moving stage to record, the corresponding sectioning image of shooting scanning element after step (6) focusing；
Step (7) repeats step (6) until the Image Acquisition of all scanning elements is completed；
After step (8) end of scan, the slice scanned is taken away, and wait and load new slice.
Compared with prior art, the beneficial effects of the present invention are:
(1) multiple slice cabinets are set inside automatic feeding device of the invention, if each slice cabinet can store dry chip, protect
Demonstrate,prove the observed quantity of slice；And be sliced storehouse in fetching device can rotate, stretch, go up and down, can automatically, efficiently realize difference
The taking-up of position slice；
(2) slice automatic sweep apparatus of the invention configures industrial camera, and industrial camera shoots amplified sectioning image,
And it is sent to scanning control system, it can be realized the automatic scanning of slice.
Detailed description of the invention
The accompanying drawings constituting a part of this application is used to provide further understanding of the present application, and the application's shows
Meaning property embodiment and its explanation are not constituted an undue limitation on the present application for explaining the application.
Fig. 1 is axonometric drawing of the invention；
Fig. 2 is main view of the invention；
Fig. 3 is top view of the invention；
Fig. 4 is slice automatic sweep apparatus axonometric drawing of the invention；
Fig. 5 is automatic feeding device axonometric drawing of the invention；
Fig. 6 is slice storehouse axonometric drawing of the invention；
Fig. 7 is automatic feeding device internal structure shaft side figure of the invention；
Fig. 8 is automatic feeding device internal structure main view of the invention；
Fig. 9 is lifting device axonometric drawing of the invention；
Figure 10 is lifting device main view of the invention；
Figure 11 is telescopic device axonometric drawing of the invention；
Figure 12 is slice getting device axonometric drawing of the invention；
Figure 13 is slice getting device main view of the invention；
Figure 14 is rotary device structure schematic diagram of the invention；
Figure 15 is inside and outside swiveling gear mechanism structure schematic diagram of the invention；
Figure 16 is rotary motor configuration schematic diagram of the invention；
Figure 17 is slice cabinet side view of the invention；
Figure 18 is slice cabinet top view of the invention；
Figure 19 is top cover axonometric drawing of the invention；
Figure 20 is bottom plate axonometric drawing of the invention；
Figure 21 is control flow chart of the invention；
Wherein, 1- automatic feeding device, 2- are sliced automatic sweep apparatus, 3- dropping liquid conduit, 4- peristaltic pump, 5- dropping liquid appearance
Device, 6-Z axis focus control, 7-Y spindle motor, 8- slice slot, 9- objective table, 10-X spindle motor, 11- object lens switch, 12- are micro-
Mirror adapter, 13- industrial camera, 14- microscope, 15- are sliced storehouse, 16- telescopic device, 17- groove, 18- lifting device, 19-
Rotating device, 20- fetching device, 21- lifting motor, the first sliding block of 22-, 23- guide rod, the first lead screw of 24-, 25- telescope motor,
The second sliding block of 26-, the second lead screw of 27-, 28- supporting plate, 29- upper clip, 30- lower clamping piece, 31- electromagnet, 32- bolt, 33- rotation
Motor, swiveling gear mechanism inside and outside 34-, 35- turntable, 36- gear, 37- are sliced cabinet, and 38- is sliced reserve tank, 39- extraction tank,
40- top cover, 41- bottom plate.
Specific embodiment
It is noted that following detailed description is all illustrative, it is intended to provide further instruction to the application.Unless another
It indicates, all technical and scientific terms used herein has usual with the application person of an ordinary skill in the technical field
The identical meanings of understanding.
It should be noted that term used herein above is merely to describe specific embodiment, and be not intended to restricted root
According to the illustrative embodiments of the application.As used herein, unless the context clearly indicates otherwise, otherwise singular
Also it is intended to include plural form, additionally, it should be understood that, when in the present specification using term "comprising" and/or " packet
Include " when, indicate existing characteristics, step, operation, device, component and/or their combination.
As background technique is introduced, the deficiency for sections observation existing in the prior art time-consuming, precision is not high, in order to
Technical problem as above is solved, present applicant proposes a kind of slice automatic scanning system and methods.
In a kind of typical embodiment of the application, as shown in Fig. 1-Figure 20, a kind of slice automatic scanning is provided
System, including automatic feeding device 1 and slice automatic sweep apparatus 2, wherein automatic feeding device 1 includes lifting device 18, stretches
Compression apparatus 16, fetching device 20, rotating device 19, slice storehouse 15 and slice cabinet 37, slice automatic sweep apparatus 2 include microscope
14, imaging device and automatic liquid-dropping mechanism.
The slice storehouse 15 be cylindrical type, inside have cavity, side wall along its circumferentially setting it is several be spaced apart it is recessed
Slot 17, the groove 17 opens up vertically downwards from 15 top of slice storehouse, and has certain depth；It places and cuts in groove 17
Piece cabinet 37, slice 37 shape of cabinet are adapted with groove 17.
Slice 15 side of storehouse offers the outlet for being sliced output.
The outlet can be the opening of rectangle, circle, ellipse or other shapes.
Preferably, the outlet is rectangle, is distributed more slice cabinets to be sliced storehouse 15.
The inside setting multilayer of slice cabinet 37 is used to store the slice reserve tank 38 of slice, is sliced to be equipped at the top of cabinet 37 and extract
Slot 39.
The extraction tank 39 is two grooves arranged side by side, picks and places slice cabinet 37 convenient for operator.
Top cover 40 is installed at the top in the slice storehouse 37, and bottom plate 41 is arranged in bottom.
Rotating device 19 is installed on 41 top of bottom plate for being sliced storehouse 37, and 19 top of rotating device is installed lifting device 18, passed through
Rotating device 19 can drive lifting device 18 to rotate；Telescopic device 16 is installed in 18 side of lifting device, installs on telescopic device 16
Fetching device 20, the realization fetching device 20 of telescopic device 16 move horizontally, above and below the realization fetching device 20 of lifting device 18
It is mobile.
The rotating device 19 includes rotating electric machine 33, inside and outside swiveling gear mechanism 34 and turntable 35, rotating electric machine 33
A gear 36 is fixed on motor shaft, the gear 36 is engaged with the internal gear of inside and outside swiveling gear mechanism 34, and the internal tooth
The fixed turntable 35 in the top of wheel, the rotation of turntable 35 is realized by rotating electric machine 33.
The lifting device 18 uses guiding rail mechanism, including the first lead screw 24, lifting motor 21, guide rod 23 and first
Sliding block 22, the first sliding block 22 are connected with the first lead screw 24, and the two sides of the first sliding block 22 have the guide rod 23 of its guiding role, the
The top of one lead screw 24 connects lifting motor 21.
The telescopic device 16 uses screw mechanism, including telescope motor 25, the second lead screw being connected with telescope motor 25
27, the second sliding block 26 is set on the second lead screw 27；Second sliding block, 26 side is installed by fetching device 20.
(wherein, rotating electric machine 33, lifting motor 21, telescope motor 25 are named only for the purposes of differentiation, do not assign it
His meaning).
The fetching device 20 includes supporting plate 28, and 28 bottom of supporting plate is fixed with the upper clip 29 and lower folder of interval set distance
Piece 30 is connected between upper clip 29 and lower clamping piece 30 by bolt 32, and the bottom of upper clip 29 is fixed with electromagnet 31；Electromagnet
When 31 energization, upper clip 29, the clamping of lower clamping piece 30 are sliced the slice in cabinet 37, when electromagnet 31 powers off, upper clip 29, lower clamping piece
30 decontrol slice.
Imaging device is installed at the top of microscope 14, and automatic liquid-dropping mechanism is installed in the side of microscope 14；The automatic drip
Liquid device includes dispensing container 5, dropping liquid conduit 3 and peristaltic pump 4, and the peristaltic pump 4 passes through dropping liquid conduit 3 and 5 phase of dispensing container
Even, the outlet end of dropping liquid conduit 3 corresponds to the top of objective table 9.
Dropping liquid can be sucked dropping liquid conduit 3 from dispensing container 5 when working by peristaltic pump 4, realize that the drop of dropping liquid stops controlling；Due to
The dropping liquid capacity of every turn of peristaltic pump 4 sucking is fixed, and the angle of operation by controlling peristaltic pump 4 can realize being precisely controlled for dropping liquid.
The imaging device is industrial camera 13, and industrial camera 13 passes through Microscope Adapter 12 and one of microscope 14
Eyepiece is connected；Industrial camera 13 shoots amplified sectioning image, and is sent to scanning control system.
The objective table 9 of microscope 14 is equipped with the slice slot 8 for placing slice, and objective table 9 passes through X-axis motor respectively
10, the control of y-axis motor 7 is moved along X-axis, Y direction.
In the fixed Z axis focus control 6 of original microscope fine tuning wheel side, control microscope fine adjustment knob is rotated by motor
Rotation, completes the focal adjustment of Z axis.
Microscope 14 is equipped with lighting system, including headlamp, mains lighting supply, and headlamp issues bright source, is irradiated to poly-
On light device, then it is sliced through light passing Aperture Illumination；Condenser is located on the condenser frame below dressing table, adjusts the light source for penetrating light hole
Amount.
The application is sliced the control method of automatic scanning system, for scanning chromosome slice, as shown in figure 21, packet
Include following steps:
The objective table 9 of the mobile microscope 14 of step (1), receives the slice through the outlet of automatic feeding device 1 output；
Slice is moved to below object lens mouth by step (2) moving stage 9, object lens switch 11 is adjusted, by low power objective
Switch to working condition；
Step (3) adjusts microscope Z axis, shoots sectioning image by industrial camera 13, and judge whether to focus；
Step (3) are repeated when step (4) is not focused, slice scanning is realized by moving stage 9 to defocused, judges to scan
Polish colour solid whether metacinesis, if not continuing to scan on, if then writing scan point coordinate；
After step (5) end of scan, object lens switch 11 is adjusted, high power objective is switched into working condition；
Step (6) continues to adjust microscope Z axis, take pictures by industrial camera 13, observe the sectioning image shot etc.
It operates, after the end of scan, moving stage 9 takes slice away；
Step (7), which waits, loads new slice.
The foregoing is merely preferred embodiment of the present application, are not intended to limit this application, for the skill of this field
For art personnel, various changes and changes are possible in this application.Within the spirit and principles of this application, made any to repair
Change, equivalent replacement, improvement etc., should be included within the scope of protection of this application.
Claims (10)
1. a kind of slice automatic scanning system characterized by comprising
Automatic feeding device, including the slice storehouse for accommodating slice cabinet, being sliced to be equipped with inside storehouse can rotate, go up and down, stretching
The fetching device of contracting；Fetching device in-built electrical magnet, slice when electromagnet is powered in clamping slice cabinet；
It is sliced automatic sweep apparatus, receives the slice that takes out through fetching device and fixation, including microscope and is installed on microscope
The automatic liquid-dropping mechanism of side；The microscope is configured like equipment, and imaging device shoots amplified sectioning image, and sends
To scanning control system.
2. a kind of slice automatic scanning system according to claim 1, which is characterized in that the fetching device is by stretching
Compression apparatus is connected with lifting device, and the lifting device is installed on above rotating device.
3. a kind of slice automatic scanning system according to claim 1 or 2, which is characterized in that the fetching device packet
Supporting plate is included, the bottom of the support plate is fixed with the upper clip and lower clamping piece of interval set distance, and the bottom of upper clip is fixed with electromagnet.
4. a kind of slice automatic scanning system according to claim 2, which is characterized in that the rotating device includes turning
Disk and the inside and outside swiveling gear mechanism for driving turntable.
5. a kind of slice automatic scanning system according to claim 2, which is characterized in that the lifting device is using straight
Line guide rail mechanism, telescopic device use screw mechanism.
6. a kind of slice automatic scanning system according to claim 1, which is characterized in that the inner wall edge in the slice storehouse
Several grooves for being used to place slice cabinet are circumferentially opened up, slice storehouse side is provided with outlet.
7. a kind of slice automatic scanning system according to claim 1, which is characterized in that have inside the slice cabinet
Multilayer wall reserve tank, slice cabinet top portion are equipped with extraction tank.
8. a kind of slice automatic scanning system according to claim 1, which is characterized in that the automatic liquid-dropping mechanism packet
Dispensing container, dropping liquid conduit and peristaltic pump are included, the peristaltic pump is connected by dropping liquid conduit with dispensing container, is wriggled by control
The angle of operation of pump realizes the control to dropping liquid.
9. a kind of slice automatic scanning system according to claim 1, which is characterized in that the imaging device is industry
Camera, industrial camera are connected by Microscope Adapter with a microscopical eyepiece.
10. the control method of -9 any a kind of slice automatic scanning systems according to claim 1, which is characterized in that
The following steps are included:
The mobile microscopical objective table of step (1), receives the slice through automatic feeding device outlet output；
Slice is moved to below object lens mouth, low power objective is switched to working condition by step (2) moving stage；
Step (3) adjusts microscope Z axis, shoots sectioning image by industrial camera, and judge whether to focus；
Step (4) realizes slice scanning by moving stage to defocused, and the scanning element for recording slice specific modality region is sat
Mark；Step (3) are repeated when not focusing；
After step (5) is sliced the low power objective end of scan, high power objective is switched into working condition, and execute step (3)；Step
(6) scanning element coordinate of the moving stage to record, the corresponding sectioning image of shooting scanning element after focusing；
Step (7) repeats step (6) until the Image Acquisition of all scanning elements is completed；
After step (8) end of scan, the slice scanned is taken away, and wait and load new slice.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810960647.7A CN109164044A (en) | 2018-08-22 | 2018-08-22 | A kind of slice automatic scanning system and method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810960647.7A CN109164044A (en) | 2018-08-22 | 2018-08-22 | A kind of slice automatic scanning system and method |
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| Publication Number | Publication Date |
|---|---|
| CN109164044A true CN109164044A (en) | 2019-01-08 |
Family
ID=64896557
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810960647.7A Pending CN109164044A (en) | 2018-08-22 | 2018-08-22 | A kind of slice automatic scanning system and method |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110608999A (en) * | 2019-10-11 | 2019-12-24 | 宁波江丰生物信息技术有限公司 | Automatic objective conversion device of digital pathological scanner |
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| CN203798718U (en) * | 2013-08-06 | 2014-08-27 | 宁波江丰生物信息技术有限公司 | Multiple-slice loading system |
| CN105318660A (en) * | 2014-07-29 | 2016-02-10 | 青岛海尔智能技术研发有限公司 | Wine cabinet allowing wine bottle to be stored and taken out automatically and stored wine information recording method of wine cabinet |
| CN107688232A (en) * | 2017-10-19 | 2018-02-13 | 江苏硕世生物科技股份有限公司 | A kind of microscope carrier, microscope and the method for capturing slide |
| CN208705216U (en) * | 2018-08-22 | 2019-04-05 | 滨州医学院 | A kind of slice automatic scanning system |
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2018
- 2018-08-22 CN CN201810960647.7A patent/CN109164044A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5103338A (en) * | 1990-10-04 | 1992-04-07 | Crowley Kevin D | Apparatus for positioning objects for microscopic examination |
| CN103033408A (en) * | 2013-01-09 | 2013-04-10 | 山东英才学院 | Device and method for remotely obtaining digital slice from glass slice |
| CN203798718U (en) * | 2013-08-06 | 2014-08-27 | 宁波江丰生物信息技术有限公司 | Multiple-slice loading system |
| CN105318660A (en) * | 2014-07-29 | 2016-02-10 | 青岛海尔智能技术研发有限公司 | Wine cabinet allowing wine bottle to be stored and taken out automatically and stored wine information recording method of wine cabinet |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110608999A (en) * | 2019-10-11 | 2019-12-24 | 宁波江丰生物信息技术有限公司 | Automatic objective conversion device of digital pathological scanner |
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