CN109136135A - A kind of preparation method of septic tank microbial bacterial agent - Google Patents

A kind of preparation method of septic tank microbial bacterial agent Download PDF

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Publication number
CN109136135A
CN109136135A CN201810994520.7A CN201810994520A CN109136135A CN 109136135 A CN109136135 A CN 109136135A CN 201810994520 A CN201810994520 A CN 201810994520A CN 109136135 A CN109136135 A CN 109136135A
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septic tank
bacterial agent
microbial bacterial
preparation
day
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胡次兵
张桂芳
史志新
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Foshan Science And Technology Co Ltd
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Foshan Science And Technology Co Ltd
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20Bacteria; Culture media therefor

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  • Chemical & Material Sciences (AREA)
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  • General Health & Medical Sciences (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Treatment Of Sludge (AREA)

Abstract

The present invention relates to a kind of preparation methods of septic tank microbial bacterial agent, belong to technical field of biological environmental protection.The present invention provides organic carbon using coconut husk, palm shell, cornstalk, sawdust, it is mixed with septic tank excrement, marc, percolation liquid of city life garbage landfill, inorganic nitrogen part is converted into gaseous nitrogen atmosphere, reduce the generation of foul smell, simultaneously using a large amount of microorganisms are contained in percolate, by adjusting microbial nutrition element, microorganism is activated, the tolerance for improving microorganism, improves the degradation efficiency of biosystem;By modulating matrix content, a large amount of carbon source and the energy are provided to promote the growth of microorganism, some degrading enzymes are come second level substance of degrading, reach higher degradation rate, gets along amiably and peacefully between them, coordinative role, wherein microorganism is converted into incomplete oxidation product using level-one substance decomposition, this product reacts with part second level substance, and product is utilized by other microorganisms again, the progress for promoting septic tank fermentation to decompose.

Description

A kind of preparation method of septic tank microbial bacterial agent
Technical field
The present invention relates to a kind of preparation methods of septic tank microbial bacterial agent, belong to technical field of biological environmental protection.
Background technique
Traditionally urban night soil has been used as agricultural fertilizer, but in the latest 20 years, since many reasons make urban night soil agriculture With occurring sharply declining, urban night soil is losing rapidly traditional clay treatment approach, and the excrement largely collected is had to Topple over near cities region, carrys out serious influence to environment and health care belt, caused social common concern.
Although urban night soil processing also had some mature technologies, as chemical method, high pressure-temperature method, During High-Temperature Composting method, The methods of septic tank processing, but chemical method be easy to cause the rare popularization and application of secondary pollution, and high pressure-temperature method and energy-saving and emission-reduction are not Match and face popularization problem, During High-Temperature Composting method because it is agricultural occur sharply decline and to disease popularity worry be restricted, change Manure pit processing is the method generallyd use both at home and abroad at present, can be also, especially landfill place election also becomes more and more difficult.It is real For border situation also in this way, in China, urban night soil is to be closed by every household premises toilet urinal and sanitary wastewater pipeline one It plays inflow septic tank to be handled, using this processing, on the one hand heavy excrement can be deposited in septic tank, general to use Draw outward transport landfill stabilization clearly, except need to spend it is high the expenses such as draw, transport and fill clearly, also cause secondary environmental pollution, and Also often because taste is smelly during septic tank cleaning and safety accident badly occurs for working environment;On the other hand, into septic tank specific gravity Small excrement is dissociated into floccule by block quickly, in suspension pool water within, is gone out together in company with the waste water entered to public row Dirt pipe, finally enters river, pollutes rivers.Therefore, subsequent processing technology is developed on the basis of the facility, avoids polluting hair again It is generated as the task of top priority.
At abroad, urban night soil decrement simultaneously get up to be broadly divided into urban drainage pipe network and sewage by harmless treatment general introduction Treatment plant as pneumatic push-pull type and processing basis sewage excrement merging treatment high cost pattern, sewage excrement merging treatment it Outside, the middle cost mould that fortune centralized processing is individually handled with the excrement that situ purification pond decentralized processing combines also is received using vehicle Formula, common ground are still exactly to face to draw clearly, transport and fill and may cause environmental pollution again.
In conclusion exploitation can rely on existing utility, does not increase existing expense, and urban night soil can be effectively treated, avoid The new technology that may cause environmental pollution again is drawn, transports and filled clearly, social development necessity.Therefore, it adds micro- Bacteria agent has very big economic benefit and extensive market prospects for septic tank excrement mud decrement, develops a kind of honest and clean The efficient bacteria agent of valence is particularly important.
Summary of the invention
The technical problems to be solved by the invention: for existing manure pit processing mode need to spend it is high draw clearly, transport and The expenses such as landfill, provide a kind of preparation method of septic tank microbial bacterial agent at the problem of also causing secondary environmental pollution.
In order to solve the above technical problems, the technical solution adopted by the present invention is that:
(1) coconut husk, palm shell, cornstalk, sawdust are taken, 20~30min in the limewash that pH is 14 is immersed in, takes out and drain, Overlay film banks up processing 7~10 days at 35~50 DEG C again, obtains activation organic carbon;
(2) septic tank excrement, marc, percolation liquid of city life garbage landfill, activation organic carbon are taken, is fitted into spice bucket and mixes It is banked up after closing uniformly, adding deionized water to water content is 50~60%, obtains fermentation heap;
(3) 30 DEG C of fermentation heap initial temperature are controlled, is heated up 10 DEG C daily within the 1st~3 day, 60 DEG C of high temperature of maintenance in the 4th~7 day, the 8th day 55 DEG C are maintained, is cooled within the 9th~11 day 45 DEG C, maintains 45 DEG C within the 12nd~15 day, is cooled within the 16th~18 day 35 DEG C, the 19th~28 It maintains 35 DEG C, obtains fermentation material;
(4) 0.5~1.2kg fermentation material is taken, is added in 5~12kg deionized water, 20~30min is stirred with 300~400r/min, It is transferred to 5~8min of centrifuge separation in centrifugal separator again, takes upper phase, obtains septic tank microbial bacterial agent.
The coconut husk, palm shell, cornstalk, sawdust mass ratio be 1:1:1:1.
The coconut husk, palm shell, cornstalk, sawdust are the powder for being crushed to 0.1~0.5cm.
The septic tank excrement, marc, percolation liquid of city life garbage landfill, activate organic carbon parts by weight be 300~500 parts of septic tank excrement, 300~500 fruits slags, 200~300 parts of percolation liquid of city life garbage landfill, 200 ~300 parts of activation organic carbons.
The septic tank excrement water content is 50~60%.
The marc is to air-dry to moisture content 1~5%, then crushed the marc of 20 meshes.
The percolation liquid of city life garbage landfill is the landfill percolate for sieving with 100 mesh sieve strainer.
The fermentation material and the mass ratio of deionized water are 1:10.
The present invention is compared with other methods, and advantageous effects are:
(1) present invention provides organic carbon using coconut husk, palm shell, cornstalk, sawdust, by itself and septic tank excrement, marc, city The mixing of city's consumer waste landfill percolate, organic carbon source is not only that carbon matrix is served as in denitrification, aerobic also by biology Breathing creates the anaerobism microhabitat for supporting denitrification, inorganic nitrogen part is converted into gaseous nitrogen atmosphere, reduces the production of foul smell It is raw, while using a large amount of microorganisms are contained in percolate, mainly there are nitrite bacteria, denitrifying bacteria, desulfurization bacterium, denitrogenation sulphur Bacillus, iron bacteria, sulfate reducing bacteria and 7 class bacterium of methanogen activate micro- life by adjusting microbial nutrition element Object, at the same by marc plant polyose and the fermentations such as animal protein digest into nutrient matrix, contain biological polyoses, amino Acid and microbial growth promoters can effectively facilitate the fast breeding of microorganism in percolate, improve the tolerance of microorganism Property, enhance the activity of microorganism, improves the degradation efficiency of biosystem;
(2) present invention is by modulation matrix content, to the decomposition and consumption of the level-one substance of easy degradation, provide a large amount of carbon source and The energy promotes the growth of microorganism, can generate the enzymes such as a large amount of protease, lipase, dehydrogenase cellulase and amylase System realizes that the ammoniation of protein, the decomposition of glucide, metabolism of the carbohydrate through related microorganisms can also be difficult to degrade two Grade substance provides necessary reducing power and various coenzyme, the decomposition of fat, the formation of humus, while also generating some degrading enzymes Come second level substance of degrading, reach higher degradation rate, constitute complicated and stable microbial community structure system, between them It gets along amiably and peacefully, coordinative role, wherein microorganism is converted into incomplete oxidation product, this product and portion using level-one substance decomposition Point second level substance reacts, and product is utilized by other microorganisms again, the progress for promoting septic tank fermentation to decompose.
Specific embodiment
Take 3~5kg coconut husk, 3~5kg palm shell, 3~5kg cornstalk, 3~5kg sawdust is immersed in the lime that pH is 14 20~30min in water takes out and drains, then overlay film banks up processing 7~10 days at 35~50 DEG C, obtains activation organic carbon, takes 300 ~500g septic tank excrement, 300~500g marc, 200~300g percolation liquid of city life garbage landfill, 200~300g Organic carbon is activated, is fitted into spice bucket and banks up after mixing, adding deionized water to water content is 50~60%, must be fermented Heap controls 30 DEG C of fermentation heap initial temperature, and preceding 3d heats up 10 DEG C daily, 60 DEG C of high temperature of maintenance in the 4th~7 day, maintains 55 within the 8th day DEG C, continuous cooling is 10 DEG C total within the 9th~11 day, 45 DEG C of maintenance in the 12nd~15 day, the 16th~18 day 10 DEG C of continuous cooling, the 19th~ It maintains 35 DEG C within 28 days, obtains fermentation material, take 0.5~1.2kg fermentation material, be added in 5~10kg deionized water, with 300~400r/ Min stirs 20~30min, then is transferred to 5~8min of centrifuge separation in centrifugal separator, takes upper phase, obtains septic tank with micro- life Object microbial inoculum.
Example 1
Take 3kg coconut husk, 3kg palm shell, 3kg cornstalk, 3kg sawdust is immersed in 20min in the limewash that pH is 14, takes out simultaneously It drains, then overlay film banks up processing 7 days at 35 DEG C, obtains activation organic carbon, takes 300g septic tank excrement, 300g marc, 200g Percolation liquid of city life garbage landfill, 200g activate organic carbon, be fitted into spice bucket and bank up after mixing, add from Sub- water to water content is 50%, obtains fermentation heap, controls 30 DEG C of fermentation heap initial temperature, and preceding 3d heats up 10 DEG C daily, is maintained within the 4th day 60 DEG C of high temperature, 55 DEG C are maintained within the 8th day, continuous cooling is 10 DEG C total within the 9th day, maintains 45 DEG C within the 12nd day, the 16th day continuous cooling 10 DEG C, it maintains 35 DEG C within the 19th day, obtains fermentation material, take 0.5kg fermentation material, be added in 5kg deionized water, stirred with 300r/min 20min, then be transferred in centrifugal separator and be centrifugated 5min, upper phase is taken, septic tank microbial bacterial agent is obtained.
Example 2
Take 4kg coconut husk, 4kg palm shell, 4kg cornstalk, 4g sawdust is immersed in 25min in the limewash that pH is 14, takes out and drip It is dry, then overlay film banks up processing 8 days at 42 DEG C, obtains activation organic carbon, takes 400g septic tank excrement, 400g marc, the city 250g City's consumer waste landfill percolate, 250g activate organic carbon, are fitted into spice bucket and bank up after mixing, add deionization Water to water content is 55%, obtains fermentation heap, controls 30 DEG C of fermentation heap initial temperature, and preceding 3d heats up 10 DEG C daily, and maintenance in the 5th day is high 60 DEG C of temperature maintains 55 DEG C on the 8th day, and continuous cooling is 10 DEG C total within the 10th day, maintains 45 DEG C within the 14th day, the 17th day continuous cooling 10 DEG C, it maintains 35 DEG C within the 23rd day, obtains fermentation material, take 0.8kg fermentation material, be added in 7kg deionized water, stirred with 350r/min 25min, then be transferred in centrifugal separator and be centrifugated 7min, upper phase is taken, septic tank microbial bacterial agent is obtained.
Example 3
Take 5kg coconut husk, 5kg palm shell, 5kg cornstalk, 5kg sawdust is immersed in 30min in the limewash that pH is 14, takes out simultaneously It drains, then overlay film banks up processing 10 days at 50 DEG C, obtains activation organic carbon, takes 500g septic tank excrement, 500g marc, 300g Percolation liquid of city life garbage landfill, 300g activate organic carbon, be fitted into spice bucket and bank up after mixing, add from Sub- water to water content is 60%, obtains fermentation heap, controls 30 DEG C of fermentation heap initial temperature, and preceding 3d heats up 10 DEG C daily, is maintained within the 7th day 60 DEG C of high temperature, 55 DEG C are maintained within the 8th day, continuous cooling is 10 DEG C total within the 11st day, maintains 45 DEG C within the 15th day, the 18th day continuous cooling 10 DEG C, it maintains 35 DEG C within the 28th day, obtains fermentation material, take 1.2kg fermentation material, be added in 10kg deionized water, stirred with 400r/min 30min, then be transferred in centrifugal separator and be centrifugated 8min, upper phase is taken, septic tank microbial bacterial agent is obtained.
Septic tank prepared by the present invention is tested with microbial bacterial agent.
Detection method: (average to generate sewage daily in three, the middle school the Changzhou Qian Huang septic tank of more than 3000 teachers and students first More than 150 cubic metres) and Feitian bonded area septic tank (average to generate more than 80 cubic metres of sewage daily), it is set not influencing septic tank In the case where applying, septic tank microbial bacterial agent of the invention is pressed into one thousandth in the first slot of septic tank, the second slot in three times Ratio inoculation.After seven days, sprayed daily in all lavatories in 3/10000ths ratio, continuous ten days.Result before and after the processing Detecting COD removal rate through environmental protection administration is 81.6%, sludge reduction 67%, the stink before lavatory is non-treated, and surface sludge is micro- Biodegrade, water quality are so clear that you can see the bottom.

Claims (8)

1. a kind of preparation method of septic tank microbial bacterial agent, which is characterized in that specific preparation step are as follows:
(1) coconut husk, palm shell, cornstalk, sawdust are taken, 20~30min in the limewash that pH is 14 is immersed in, takes out and drain, Overlay film banks up processing 7~10 days at 35~50 DEG C again, obtains activation organic carbon;
(2) septic tank excrement, marc, percolation liquid of city life garbage landfill, activation organic carbon are taken, is fitted into spice bucket and mixes It is banked up after closing uniformly, adding deionized water to water content is 50~60%, obtains fermentation heap;
(3) 30 DEG C of fermentation heap initial temperature are controlled, is heated up 10 DEG C daily within the 1st~3 day, 60 DEG C of high temperature of maintenance in the 4th~7 day, the 8th day 55 DEG C are maintained, is cooled within the 9th~11 day 45 DEG C, maintains 45 DEG C within the 12nd~15 day, is cooled within the 16th~18 day 35 DEG C, the 19th~28 It maintains 35 DEG C, obtains fermentation material;
(4) 0.5~1.2kg fermentation material is taken, is added in 5~12kg deionized water, 20~30min is stirred with 300~400r/min, It is transferred to 5~8min of centrifuge separation in centrifugal separator again, takes upper phase, obtains septic tank microbial bacterial agent.
2. a kind of preparation method of septic tank microbial bacterial agent as described in claim 1, which is characterized in that the coconut husk, Palm shell, cornstalk, sawdust mass ratio be 1:1:1:1.
3. a kind of preparation method of septic tank microbial bacterial agent as described in claim 1, which is characterized in that the coconut husk, Palm shell, cornstalk, sawdust are the powder for being crushed to 0.1~0.5cm.
4. a kind of preparation method of septic tank microbial bacterial agent as described in claim 1, which is characterized in that the septic tank Excrement, percolation liquid of city life garbage landfill, activates the parts by weight of organic carbon as 300~500 parts of septic tank excrement at marc Just, 300~500 fruits slag, 200~300 parts of percolation liquid of city life garbage landfill, 200~300 parts of activation organic carbons.
5. a kind of preparation method of septic tank microbial bacterial agent as described in claim 1, which is characterized in that the septic tank Excrement water content is 50~60%.
6. a kind of preparation method of septic tank microbial bacterial agent as described in claim 1, which is characterized in that the marc To air-dry to moisture content 1~5%, then it crushed the marc of 20 meshes.
7. a kind of preparation method of septic tank microbial bacterial agent as described in claim 1, which is characterized in that the city is raw Percolate from garbage filling field living is the landfill percolate for sieving with 100 mesh sieve strainer.
8. a kind of preparation method of septic tank microbial bacterial agent as described in claim 1, which is characterized in that the fermentation material Mass ratio with deionized water is 1:10.
CN201810994520.7A 2018-08-29 2018-08-29 A kind of preparation method of septic tank microbial bacterial agent Pending CN109136135A (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110540302A (en) * 2019-05-31 2019-12-06 广东五色时空生态环境科技有限公司 Pollution source gridding biological intervention pollution-reducing deodorizing process

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US8524085B2 (en) * 2011-01-10 2013-09-03 City Of Newberg Sewage composting method
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CN108329144A (en) * 2018-04-23 2018-07-27 戴琪 A kind of preparation method of Beer Sludge biological nutritive agent

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110540302A (en) * 2019-05-31 2019-12-06 广东五色时空生态环境科技有限公司 Pollution source gridding biological intervention pollution-reducing deodorizing process

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