CN109134589A - A kind of extracting method of tea protein product - Google Patents
A kind of extracting method of tea protein product Download PDFInfo
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- CN109134589A CN109134589A CN201810842070.XA CN201810842070A CN109134589A CN 109134589 A CN109134589 A CN 109134589A CN 201810842070 A CN201810842070 A CN 201810842070A CN 109134589 A CN109134589 A CN 109134589A
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- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/145—Extraction; Separation; Purification by extraction or solubilisation
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Abstract
The present invention provides a kind of extracting method of tea protein product, specific method is the following steps are included: 1) pretreatment of raw material process;2) process of extraction with ultrasonic-NaOH tea leaf protein is utilized;3) process of tea leaf protein solution decoloration;4) tea leaf protein extracting solution concentration process;5) process of tea leaf protein precipitating;6) tea leaf protein washing, purification and dry process.Beneficial effect of the present invention: the present invention uses the low-grade tea such as tea grounds or Summer-autumn tea for raw material, using low frequency cycle ultrasonic technique assisted extraction tea albumen, the method extraction rate of protein is high, purity is high, method is at low cost, simple and easy, easy to spread, the color of gained tea protein product is green tea color, and product quality meets food hygienic standard.The present invention provides the new ways that tea grounds and summer and autumn low grade tea increment conversion recycle;A kind of novel plant-derived albumen new product is developed, tea resources comprehensive utilization and intensive processing are realized.
Description
Technical field
The present invention relates to the development and utilization field of plant product, mainly a kind of extracting method of tea protein product.
Background technique
China is Tea Production big country, and tea industry is quickly grown, and tea place area and yield constantly increase, and tea resource confession occurs
Greater than the trend asked.It is counted according to FAO-IGG, global tea yield reaches 392.84 ten thousand tons within 2009.Nearly 10 years Chinese teas produce
Sustainable growth is measured, between 2000 to 2007, tea yield rises to 1,140,000 tons from 71.93 ten thousand tons, and average growth rate per annum is close
11%, it has reached 1,310,000 tons within 2009, is maximum Chan Cha state in the world.And Tea Consumption is from 45.6 ten thousand tons of 2000 increases
It is average annual to increase less than 6% to 660,000 tons in 2006.Analyze its reason, on the one hand, due to the general situation of development of China's Tea Industry
It is that Famous High-quality Tea is wide in variety, consumption figure is big;And most of low-grade tea Summer-autumn tea is because of the reasons such as low, picking labour cost height of price
A large amount of tea resources are caused to stay to adopt and be not used;Another aspect tealeaves functionality extract industry and tea beverage industry development are fast
Speed, the modern production line of kiloton is gone into operation in succession in annual output, and this generates a large amount of tea waste residues, these tea grounds stock numbers are very
Greatly, but by the end of there is presently no effectively development and utilization measures, the huge waste of tea resource is not only caused, is also simultaneously
Very big pollution to environment, therefore a large amount of tea grounds and Summer-autumn tea have been processed into urgent problem.And it is reasonably comprehensive sharp
With and deep development technology have good effect for increasing Tea Industry chain economic value and environmental protection, and meet
The key of national tea industry development strategy demand.
The comprehensive utilization of low-grade tea is concentrated mainly in tealeaves in the research of soluble component at present.Such as: Wang Shu
Hero et al. is application No. is 96113134.9 patents of invention: the method that tea polyphenols and its byproduct are extracted in tealeaves, Li Zheng
Hero is application No. is the patents of invention of 91103403.X: water soluble tea extracts, and Ye Chunyuan is application No. is 93102630.X
Patent of invention: comprehensive utilization method of tea waste.But the extracted substance of these deep processings only accounts for the 30% of dry weight of tea leaves
Left and right, the ingredient utilized are mainly tea polyphenols, caffeine, soluble saccharide and vitamin etc., it is processed after tea grounds in still
The tea polyphenols of residual 1% ~ 2%, 0.1% ~ 0.3% caffeine, 17% ~ 25% crude protein, 16% ~ 18% crude fibre.China is current
Also is still in the recycling of tea grounds, existing invention is concentrated mainly on tea grounds and is used as feed, fertilizer the Primary Study stage
In research, such as: Tu Youying et al. is application No. is 200510049878.5 patents of invention: with discarded tea grounds fermenting and producing tea
The method of garden fertilizer special for organic.
Protein content is about 17 ~ 30% in tealeaves or tea grounds, and amino acid composition is abundant rationally, rich in multivitamin and
Microelement, it is cholesterol-free, there are a variety of physiological active functions, be a kind of good leaf protein resource.But tealeaves forms
Extremely complex, in tea leaf protein production and processing, thermophysical effect will lead to Starch Hydrolysis, glucide conversion, albumen and sugar
Substance combines, and a series of chemical changes such as polyphenols oxidation, protein product color change is serious, and color is deeper, and
Tea albumen is mostly sedimentable protein, causes extraction rate of protein lower.The presence of these problems makes at present to tea leaf protein
Research and utilization be all also in the conceptual phase in laboratory, such as Shen Lian clear et al. application No. is 200810164171.2 hairs
Bright patent: the double-enzyme extraction method of tea leaf protein, recovery rate are only up to 52.3%, and albumen enzyme dosage is big, expensive, it is difficult to work
Industry metaplasia produces and application;Yu Fang is application No. is 200910181276.3 patents of invention: a kind of selenium protein from selenium-enriched green tea mentions
Method is taken, but its extraction time is 16h, extraction time is long, and the production cycle is long.Simple technique, cheap cost and high
Rate be the key that tea albumen can merchandized handling, be of great significance to the deep development of tea industry.It is looked into newly, both at home and abroad still
Have not seen the research report or patent disclosure of tea protein product ultrasonically auxiliary extracting method.
Summary of the invention
The purpose of the present invention is provide for shortcoming present in the comprehensive utilization techniques such as low-grade tea, tea grounds now
A kind of extracting method of tea protein product, this extracts the method and process letter of wherein tea albumen using ultrasound assisted extraction technology
Single, production cost is low, and tea protein extracting ratio is high.
It is another object of the present invention to provide a kind of tea protein extract prepared using the above method, the product eggs
White matter content is 50% -65%, and color is green tea color, and amino acid content is abundant, and composition compares balance.
The technical scheme adopted by the invention to solve the technical problem: the extracting method of this tea protein product, this method
Including the following steps:
(1) pretreatment of raw material process: cleaning removal of impurities dephenolize is carried out using tealeaves or tea grounds as raw material, crushed after being dried is spare;
(2) tea albumen leaching process: low frequency ultrasound assists alkali carries, and tea egg white matter extracting solution, detailed process are as follows: to raw material is made
The lye stirring and leaching of the middle pH10-13 that 20~40 times of weight is added, using low frequency ultrasound auxiliary extraction technology, wherein ultrasonic wave
Condition are as follows: ultrasonic power: 200~300W, supersonic frequency: 23.5~28.5Hz;Extraction conditions are as follows: 40~80 DEG C of feed liquid temperature,
30~90min of extraction time;
(3) decolorization is carried out to tea egg white matter extracting solution;
(4) concentration is carried out to tea egg white matter extracting solution, concentrate is made;
(5) tea albumen is settled out from concentrate;
(6) tea albumen is washed, refined and is dried, tea protein product is made.
Preferably, step (1) pretreatment of raw material process, wherein raw material is respectively 0.01-0.03% through volume by volume concentration
EDTA-2Na aqueous solution, i.e. disodium ethylene diamine tetra-acetic acid aqueous solution and volume by volume concentration are the washing of 35-75% ethanol solution, removal
Dust, metal ion and part phenolic substances in raw material, it is dry after draining.
Preferably, step (1) raw material crushing process, wherein raw material crosses 100-200 mesh after pulverizer crushes, low
4 DEG C of temperature is in store for.
Preferably, step (3) carries out decolorization to tea egg white matter extracting solution are as follows: to every 100ml Protein Extraction
Be slowly added in liquid 2~7ml volume by volume concentration be 30% hydrogen peroxide solution, be stirred at 20~40 DEG C reaction 30~
80min。
Preferably, the process that tea leaf protein extracting solution is concentrated in step (4) are as follows: using vacuum concentration, temperature control
System is concentrated into the 20-50% of original volume at 40-60 DEG C.
Preferably, step (5) precipitates the process of tea albumen from tea egg white matter extracting solution are as follows: to the albumen after concentration
HCl is added in matter extracting solution, pH is adjusted to 3~5, stands 30~60min, precipitate protein sufficiently, 3000-10000r/
The centrifugal force of min separates 20-40min, discards supernatant liquid, obtains protein precipitation.
Preferably, the process that step (6) is washed tea albumen, refined and dried are as follows: to above-mentioned resulting albumen
The pure water of 10~50 times of Sediment weights is added in matter precipitating, washs 2~5 times at room temperature, 3000-10000r/min centrifuge separation
20min, obtains protein, is added the pure water of 3~10 times of weight, agitated adjusting pH to neutrality, after colloid mill and homogenizer,
It is spray-dried or is freeze-dried and to obtain tea protein product.
The present invention has the advantages that the present invention extracts tea albumen using low frequency cycle ultrasonic assisted technique, hot water is improved
The traditional handicraft for bathing alkalinity extraction albumen, overcomes the drawback that alkali lye consumption amount is big, extraction rate of protein is low, with traditional hot water
Bath alkalinity extraction technique is compared, and improves the recovery rate of protein and the purity of albumen, the recovery rate of albumen up to 80% or more,
Lipidated protein is up to 60% or more in tea protein extract.Meanwhile the tea albumen of the method for the present invention preparation, after decoloration process,
Product is green tea color, improves the defect that tea protein product obtained by traditional alkaline extraction is dark brown brown.Gained tea protein product exists
There is good water imbibition (3.7g/g), oil absorption (4.0g/g) within the scope of entire pH;Emulsibility, emulsification are steady when pH8.0 or more
Qualitative, foaming characteristic and foaming stability can and soybean protein isolate compare favourably.The present invention also has simple process, and low energy consumption, effect
Rate is high, does not need that a large amount of bronsted lowry acids and bases bronsted lowry is added in the process, saves water resource, extraction is at low cost, and environmental pollution is small, extraction conditions
The advantages that mild.
Specific embodiment
In order to make the objectives, technical solutions, and advantages of the present invention clearer, below with reference to citing, to the present invention into
Row is further described.It should be appreciated that citing described herein is only used to explain the present invention, it is not used to limit this hair
It is bright.
A kind of extracting method of tea protein product of 1 ﹑ of embodiment, step are as follows:
(1) tealeaves is washed through 0.01%EDTA-2Na aqueous solution and 35% ethanol solution, remove dust in raw material, metal ion and
Part phenolic substances, dry after draining, the feed moisture content 8% after drying;
(2) tealeaves crushes, and be sieved 100 mesh;
(3) low frequency cycle ultrasound assisted extraction tea albumen: weighing 1kg raw material, and the alkali that the pH of 20 times of weight is 10 is added thereto
Solution extracts the effective components such as protein therein using low frequency cycle ultrasound on extracting technology, wherein ultrasonic wave added condition
Are as follows: ultrasonic power: 300W, supersonic frequency: 24.5Hz;Extraction conditions are as follows: 40 DEG C of feed liquid temperature, extraction time 40min;It has extracted
Cheng Hou, 3000r/min are centrifuged 40min, discard residue, obtain extracting solution of protein;
(4) it decolourizes to extracting solution of protein: 3% hydrogen peroxide solution being added into extracting solution of protein, is stirred at 20 DEG C
Hybrid reaction 30min;
(5) extracting solution of protein is concentrated: using vacuum concentration, temperature is controlled at 50 DEG C, is concentrated into the 50% of original volume;
(6) tea albumen is settled out from the extracting solution of protein after decoloration concentration: adding into the extracting solution of protein after decoloration
Enter HCl and pH is adjusted to 5.0, stand 60min, precipitate protein sufficiently, 3000r/min is centrifuged 40min, discards supernatant liquid, obtains
Protein extract precipitating.
(7) tea albumen is washed, refined and dried: into above-mentioned resulting protein precipitation, 50 times of addition is pure
Water washs 2 times at room temperature, and 3000r/min is centrifugated 20min, obtains protein, and 8 times of pure water, agitated adjusting pH is added
Tea protein product is spray-dried to obtain after colloid mill and homogenizer to neutrality.
(8) tea protein extracting ratio: 80.49%, protein content in tea protein extract: 55.74%..
A kind of extracting method of tea protein product of 2 ﹑ of embodiment, step are
(1) tealeaves is washed through 0.02%EDTA-2Na aqueous solution and 50% ethanol solution, remove dust in raw material, metal ion and
Part phenolic substances, it is dry after draining;
(2) tealeaves crushes, and be sieved 150 mesh;
(3) low frequency cycle ultrasound assisted extraction tea albumen: weighing 1kg raw material, and the alkali that the pH of 30 times of weight is 12 is added thereto
Solution extracts the effective components such as protein therein using low frequency cycle ultrasound on extracting technology, wherein ultrasonic wave added condition
Are as follows: ultrasonic power: 250W, supersonic frequency: 26.5Hz;Extraction conditions are as follows: 55 DEG C of feed liquid temperature, extraction time 60min;It has extracted
Cheng Hou, 6000r/min are centrifuged 20min, discard residue, obtain extracting solution of protein;
(4) it decolourizes to extracting solution of protein: 4% hydrogen peroxide solution being added into extracting solution of protein, is stirred at 30 DEG C
Hybrid reaction 60min;
(5) extracting solution of protein is concentrated: using vacuum concentration, temperature is controlled at 40 DEG C, is concentrated into the 30% of original volume;
(6) tea albumen is settled out from the extracting solution of protein after decoloration concentration: adding into the extracting solution of protein after decoloration
Enter HCl and pH is adjusted to 4.0, stand 40min, precipitate protein sufficiently, 6000r/min is centrifuged 30min, discards supernatant liquid, obtains
Protein extract precipitating.
(7) tea albumen is washed, refined and dried: into above-mentioned resulting protein precipitation, 30 times of addition is pure
Water washs 4 times at room temperature, and 6000r/min is centrifugated 20min, obtains protein, and 5 times of pure water, agitated adjusting pH is added
Tea protein product is freeze-dried to obtain after colloid mill and homogenizer to neutrality.
(8) tea protein extracting ratio: 89.57%, protein content in tea protein extract: 60.76%.
A kind of extracting method of tea protein product of 3 ﹑ of embodiment, step are as follows:
(1) tea grounds is washed through 0.03%EDTA-2Na aqueous solution and 75% ethanol solution, remove dust in raw material, metal ion and
Part phenolic substances, it is dry after draining;
(2) tealeaves crushes, and be sieved 200 mesh;
(3) low frequency cycle ultrasound assisted extraction tea albumen: weighing 1kg raw material, and the alkali that the pH of 40 times of weight is 13 is added thereto
Solution extracts the effective components such as protein therein using low frequency cycle ultrasound on extracting technology, wherein ultrasonic wave added condition
Are as follows: ultrasonic power: 300W, supersonic frequency: 23.5Hz;Extraction conditions are as follows: 80 DEG C of feed liquid temperature, extraction time 90min;It has extracted
Cheng Hou, 10000r/min are centrifuged 20min, discard residue, obtain extracting solution of protein;
(4) it decolourizes to extracting solution of protein: 7% hydrogen peroxide solution being added into extracting solution of protein, is stirred at 25 DEG C
Hybrid reaction 80min;
(5) extracting solution of protein is concentrated: using vacuum concentration, temperature is controlled at 60 DEG C, is concentrated into the 20% of original volume;
(6) tea albumen is settled out from the extracting solution of protein after decoloration concentration: adding into the extracting solution of protein after decoloration
Enter HCl and pH is adjusted to 3.0, stand 50min, precipitate protein sufficiently, 10000r/min is centrifuged 20min, discards supernatant liquid, obtains
Protein extract precipitating.
(7) tea albumen is washed, refined and dried: into above-mentioned resulting protein precipitation, 40 times of addition is pure
Water washs 5 times at room temperature, and 10000r/min is centrifugated 20min, obtains protein, and 10 times of pure water, agitated adjusting is added
PH is spray-dried to obtain tea protein product after colloid mill and homogenizer to neutrality.
(8) tea protein extracting ratio: 91%, protein content in tea protein extract: 63.10%.
A kind of extracting method of tea protein product of 4 ﹑ of embodiment, step are as follows:
(1) tea grounds is washed through 0.03%EDTA-2Na aqueous solution and 60% ethanol solution, remove dust in raw material, metal ion and
Part phenolic substances, dry after draining, the feed moisture content 10% after drying;
(2) tealeaves crushes, and be sieved 100 mesh;
(3) low frequency cycle ultrasound assisted extraction tea albumen: weighing 1kg raw material, and the alkali that the pH of 35 times of weight is 11 is added thereto
Solution extracts the effective components such as protein therein using low frequency cycle ultrasound on extracting technology, wherein ultrasonic wave added condition
Are as follows: ultrasonic power: 300W, supersonic frequency: 28.5Hz;Extraction conditions are as follows: 50 DEG C of feed liquid temperature, extraction time 50min;It has extracted
Cheng Hou, 8000r/min are centrifuged 30min, discard residue, obtain extracting solution of protein;
(4) it decolourizes to extracting solution of protein: 5% hydrogen peroxide solution being added into extracting solution of protein, is stirred at 40 DEG C
Hybrid reaction 50min;
(5) extracting solution of protein is concentrated: using vacuum concentration, temperature is controlled at 40 DEG C, is concentrated into the 40% of original volume;
(6) tea albumen is settled out from the extracting solution of protein after decoloration concentration: adding into the extracting solution of protein after decoloration
Enter HCl and pH is adjusted to 4.0, stand 30min, precipitate protein sufficiently, 8000r/min is centrifuged 20min, discards supernatant liquid, obtains
Protein extract precipitating.
(7) tea albumen is washed, refined and dried: into above-mentioned resulting protein precipitation, 20 times of addition is pure
Water washs 4 times at room temperature, and 8000r/min is centrifugated 20min, obtains protein, and 3 times of pure water, agitated adjusting pH is added
Tea protein product is freeze-dried to obtain after colloid mill and homogenizer to neutrality.
(8) tea protein extracting ratio: 84.76%, protein content in tea protein extract: 60.04%.
A kind of extracting method of tea protein product of 5 ﹑ of embodiment, step are as follows:
(1) tea grounds is washed through 0.02%EDTA-2Na aqueous solution and 50% ethanol solution, remove dust in raw material, metal ion and
Part phenolic substances, it is dry after draining;
(2) tealeaves crushes, and be sieved 100 mesh;
(3) low frequency cycle ultrasound assisted extraction tea albumen: weighing 1kg raw material, and the alkali that the pH of 30 times of weight is 12 is added thereto
Solution extracts the effective components such as protein therein using low frequency cycle ultrasound on extracting technology, wherein ultrasonic wave added condition
Are as follows: ultrasonic power: 200W, supersonic frequency: 25.5Hz;Extraction conditions are as follows: 70 DEG C of feed liquid temperature, extraction time 30min;It has extracted
Cheng Hou, 5000r/min are centrifuged 40min, discard residue, obtain extracting solution of protein;
(4) it decolourizes to extracting solution of protein: 2% hydrogen peroxide solution being added into extracting solution of protein, is stirred at 35 DEG C
Hybrid reaction 60min;
(5) extracting solution of protein is concentrated: using vacuum concentration, temperature is controlled at 50 DEG C, is concentrated into the 50% of original volume;
(6) tea albumen is settled out from the extracting solution of protein after decoloration concentration: adding into the extracting solution of protein after decoloration
Enter HCl and pH is adjusted to 3.5, stand 30min, precipitate protein sufficiently, 5000r/min is centrifuged 40min, discards supernatant liquid, obtains
Protein extract precipitating.
(7) tea albumen is washed, refined and dried: into above-mentioned resulting protein precipitation, 10 times of addition is pure
Water washs 5 times at room temperature, and 5000r/min is centrifugated 20min, obtains protein, and 10 times of pure water, agitated adjusting is added
PH is spray-dried to obtain tea protein product after colloid mill and homogenizer to neutrality.
(8) tea protein extracting ratio: 86.50%, protein content in tea protein extract: 55.27%.
Finally it is pointed out that the above list is only a few specific embodiments of the present invention.Obviously, of the invention
Above embodiments are not limited to, acceptable there are many deformations.Above embodiment is carried out according to the concept of the present invention various equivalent
Change and modify generated all deformations, is considered as within protection scope of the present invention.
Claims (7)
1. a kind of extracting method of tea protein product, it is characterized in that: this method including the following steps:
(1) pretreatment of raw material process: cleaning removal of impurities dephenolize is carried out using tealeaves or tea grounds as raw material, crushed after being dried is spare;
(2) tea albumen leaching process: low frequency ultrasound assists alkali carries, and tea egg white matter extracting solution, detailed process are as follows: to raw material is made
The lye stirring and leaching of the middle pH10-13 that 20~40 times of weight is added, using low frequency ultrasound auxiliary extraction technology, wherein ultrasonic wave
Condition are as follows: ultrasonic power: 200~300W, supersonic frequency: 23.5~28.5Hz;Extraction conditions are as follows: 40~80 DEG C of feed liquid temperature,
30~90min of extraction time;
(3) decolorization is carried out to tea egg white matter extracting solution;
(4) concentration is carried out to tea egg white matter extracting solution, concentrate is made;
(5) tea albumen is settled out from concentrate;
(6) tea albumen is washed, refined and is dried, tea protein product is made.
2. the extracting method of tea protein product according to claim 1, it is characterised in that: step (1) pretreatment of raw material
Process, wherein raw material is respectively 0.01-0.03%EDTA-2Na aqueous solution, i.e. disodium ethylene diamine tetraacetate water through volume by volume concentration
Solution and volume by volume concentration are the washing of 35-75% ethanol solution, remove dust, metal ion and the part phenolic substances in raw material,
It is dry after draining.
3. the extracting method of tea protein product according to claim 1 or 2, it is characterised in that: step (1) raw material crushes
Process, wherein raw material crosses 100-200 mesh, 4 DEG C of low temperature are in store for after pulverizer crushes.
4. the extracting method of tea protein product according to claim 1, it is characterised in that: step (3) is to tea leaf protein
Matter extracting solution carries out decolorization are as follows: the volume by volume concentration that 2~7ml is slowly added into every 100ml extracting solution of protein is 30%
Hydrogen peroxide solution is stirred 30~80min of reaction at 20~40 DEG C.
5. the extracting method of tea protein product according to claim 1, it is characterised in that: step (4) is to tea leaf protein
The process that extracting solution is concentrated are as follows: using vacuum concentration, temperature is controlled at 40-60 DEG C, is concentrated into the 20-50% of original volume.
6. the extracting method of tea protein product according to claim 1, it is characterised in that: step (5) is from tea leaf protein
The process of tea albumen is precipitated in matter extracting solution are as follows: HCl is added into the extracting solution of protein after concentration by pH and is adjusted to 3~5, it is quiet
30~60min is set, precipitates protein sufficiently, the centrifugal force of 3000-10000r/min separates 20-40min, discards supernatant
Liquid obtains protein precipitation.
7. the extracting method of tea protein product according to claim 1, it is characterised in that: step (6) to tea albumen into
Row washing, purification and dry process are as follows: the pure of 10~50 times of Sediment weights is added into above-mentioned resulting protein precipitation
Water washs 2~5 times at room temperature, and 3000-10000r/min is centrifugated 20min, obtains protein, and the pure of 3~10 times of weight is added
Water purification, agitated adjusting pH to neutrality are spray-dried or are freeze-dried and to obtain tea protein product after colloid mill and homogenizer.
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CN116235894A (en) * | 2023-03-02 | 2023-06-09 | 大闽食品(漳州)有限公司 | Preparation method and application of tea dreg protein |
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