CN109125375B - Preparation method of Chuzhou chrysanthemum eye patch liquid for protecting eyes and improving eyesight - Google Patents
Preparation method of Chuzhou chrysanthemum eye patch liquid for protecting eyes and improving eyesight Download PDFInfo
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Abstract
The invention provides a preparation method of Chuzhou chrysanthemum eye patch liquid for protecting eyes and improving eyesight, and relates to the technical field of eye medicine processing. The preparation method of the eye patch liquid comprises the following steps: preprocessing Chuzhou chrysanthemum, extracting the total flavonoids of the Chuzhou chrysanthemum, preparing enzymolysis pearl liquid, preparing borneol-beta-cyclodextrin and preparing eye patch liquid. The invention overcomes the defects of the prior art, reduces the uncomfortable feeling of treating eye diseases by the traditional method, has the effects of protecting eyes, improving eyesight and relieving eye fatigue by the mutual matching of a plurality of natural extracts, and also has the advantages of convenient use, safe raw materials, stable effect and the like.
Description
Technical Field
The invention relates to the technical field of eye patch liquid processing, and particularly relates to a preparation method of a Chuzhou chrysanthemum eye patch liquid for protecting eyes and improving eyesight.
Background
The chrysanthemum is a dry capitate inflorescence of chrysanthemum of a Compositae plant, has the effects of dispelling wind and clearing heat, calming liver and improving eyesight, and is a common Chinese herbal medicine for clinical use in traditional Chinese medicine. The chrysanthemum is used as a pure natural Chinese herbal medicine, is used as a vision improving medicine from ancient times, has the advantage of unique time and experience in the use of ophthalmic diseases, is only used for chrysanthemum tea and other products in daily life, and makes full use of the liver calming and vision improving functions of the chrysanthemum. Modern researches show that the chrysanthemum total flavonoids have the effects of inhibiting apoptosis of lacrimal gland acinus and glandular cells, improving the basic secretion of tears, maintaining the stability of tear films and improving the dry state of eye surfaces, the mechanism of the chrysanthemum total flavonoids is probably related to the androgenic effect of the chrysanthemum total flavonoids, and the androgenic hormone has the effects of locally inhibiting apoptosis promoting factors Fas and FasL, so that the chrysanthemum total flavonoids have the effect of relieving the dry state of eyes. In addition, as an important substance for maintaining eye health, vitamin A, the data shows that the chrysanthemum is also quite rich in vitamin A.
At home and abroad, the pharmacological action of chrysanthemum has been widely researched in recent years, in the research of ophthalmic diseases, the action mechanism of chrysanthemum for treating xerophthalmia is researched by yao xiaoju and the like, Azzaroo AM, Wood RL, Mircheff AK and the like, the chrysanthemum is used as a pure natural eyesight-improving and eye-protecting medicinal material and is single in actual life application type, nowadays, the popularization of electronic products such as computers, mobile phones and the like leads eye diseases to be more and more younger, myopia, xerophthalmia and the like to be more and more frequent, and the medicines for treating the eye diseases are also endlessly developed.
Disclosure of Invention
Aiming at the defects of the prior art, the invention provides the preparation method of the Chuzhou chrysanthemum eye patch for protecting eyes and improving eyesight, which reduces the discomfort of treating eye diseases by the traditional method, and the invention has the effects of protecting eyes, improving eyesight and relieving eye fatigue by mutually matching various natural extracts, and also has the advantages of convenient use, safe raw materials, stable effect and the like.
In order to achieve the above purpose, the technical scheme of the invention is realized by the following technical scheme:
a preparation method of Chuzhou chrysanthemum eye patch liquid for protecting eyes and improving eyesight comprises the following steps:
(1) preprocessing Chuzhou chrysanthemum: preferably, intact Chuzhou chrysanthemum is dried in a dryer, then the dried Chuzhou chrysanthemum is placed in a grinder to be ground and ground, and the obtained powder is sieved to obtain Chuzhou chrysanthemum powder for later use;
(2) extracting Chuzhou chrysanthemum total flavonoids: adding the obtained Chuzhou chrysanthemum powder into 50 parts by volume of ethanol solution, performing ultrasonic extraction in an ultrasonic cleaning machine, filtering, repeating the steps for three times, combining filtrate, and concentrating the obtained filtrate to obtain a Chuzhou chrysanthemum extracting solution for later use;
(3) preparing enzymolysis pearl liquid: adding 10 volume parts of distilled water into pearl powder, adding 3 volume parts of hydrochloric acid, mixing and standing until no bubbles are generated, adding ammonia water to adjust the pH value of the solution to be neutral, heating for 40min in a water bath environment, adding papain, continuing to heat in the water bath, keeping the temperature, heating the solution to 100 ℃, continuously boiling for a period of time, cooling, fixing the volume, and refrigerating for 50 hours in a refrigerator to obtain an enzymolysis pearl solution for later use;
(4) preparing borneol-beta-cyclodextrin: accurately weighing beta-cyclodextrin and borneol according to a proportion, mixing the weighed beta-cyclodextrin with distilled water, dissolving in a water bath until the mixture is transparent, adding borneol, stirring and dissolving, carrying out ultrasonic treatment, refrigerating the mixed solution after the mixed ultrasonic treatment in a refrigerator for 24 hours, filtering, washing with absolute ethyl alcohol and distilled water for three times respectively, and drying to obtain a borneol-beta-cyclodextrin inclusion compound for later use;
(5) preparing eye patch liquid: mixing flos Chrysanthemi extractive solution, enzymolysis Margarita solution, Borneolum-beta-cyclodextrin clathrate, glycerol and Aloe water, stirring to obtain mixed eye patch solution, soaking disposable eye patch in the mixed eye patch solution, storing in refrigerator, and taking out when in use.
Preferably, the concentration of the ethanol solution used in the step (2) is 70%, the power of ultrasonic extraction of the Chuzhou chrysanthemum by the ultrasonic cleaning machine is 200W, and the ultrasonic extraction time is 20 min.
Preferably, the final filtrate obtained in the process of extracting the total flavonoids in the Chuzhou chrysanthemum is concentrated to 50 times of the volume parts of the Chuzhou chrysanthemum powder according to a proportion.
Preferably, the hydrochloric acid added in the step (3) is a 12% hydrochloric acid solution, the water bath heating temperature is 45 ℃, the added papain is half of the pearl powder in parts by weight, the water bath heating and heat preservation time is 5 hours after the papain is added, the boiling time is 15min, and the volume of the solution is 50 times of the volume of the pearl powder.
Preferably, in the step (4), the mass ratio of the borneol to the beta-cyclodextrin is 1: 6, the ultrasonic temperature is 35 ℃, the ultrasonic time is 1h, the water bath dissolution temperature is 55 ℃, the drying temperature after filtration is 40 ℃, and the drying time is 24 h.
Preferably, the mass ratio of the Chuzhou chrysanthemum extracting solution, the enzymolysis pearl solution, the borneol-beta-cyclodextrin inclusion compound, the glycerol and the aloe water in the step (5) is 20: 10: 1: 10, and the glycerol is 12.5 percent of glycerol.
Preferably, the eye patch liquid is used in the following manner: soaking the disposable eye patch in the mixed eye patch solution, refrigerating, and taking out for application.
The invention provides a preparation method of Chuzhou chrysanthemum eye patch liquid for protecting eyes and improving eyesight, which has the advantages that compared with the prior art:
(1) the chrysanthemum tea uses the Chuzhou chrysanthemum, the pearl powder and the borneol as main raw materials, wherein flavonoids substances contained in the Chuzhou chrysanthemum have the effects of dispelling wind, dissipating heat, calming the liver and improving eyesight; the pearl is always regarded as a good medicine for clearing liver, improving eyesight and beautifying, the pearl is ground into powder by the traditional method for taking, the powder is difficult to absorb, the calcium carbonate content of the pearl is high, and the pearl has certain irritation to stomach; the borneol is a common medicine for clinical resuscitation and resuscitation inducing of traditional Chinese medicine, and the borneol is used as an auxiliary medicine, has the effect of promoting transdermal absorption, and improves the bioavailability of ophthalmic medicines. But the borneol has stronger volatility, and the invention has the effect of improving the stability by performing the inclusion action of the beta-cyclodextrin and the borneol.
(2) When the beta-cyclodextrin and the borneol are cooperated, the eye patch is prepared by an ultrasonic method, wherein the ultrasonic time, the ultrasonic high temperature and the mass ratio of the beta-cyclodextrin to the borneol are all the best combination modes detected by an orthogonal experiment method, so that the finally obtained eye patch has the best curative effect.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention are clearly and completely described below in conjunction with the embodiments of the present invention, and it is obvious that the described embodiments are some embodiments of the present invention, but not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1:
a preparation method of Chuzhou chrysanthemum eye patch liquid for protecting eyes and improving eyesight comprises the following steps:
(1) preprocessing Chuzhou chrysanthemum: preferably, intact Chuzhou chrysanthemum is dried in a dryer, then the dried Chuzhou chrysanthemum is placed in a grinder to be ground and ground, and the obtained powder is sieved to obtain Chuzhou chrysanthemum powder for later use;
(2) extracting Chuzhou chrysanthemum total flavonoids: adding the obtained Chuzhou chrysanthemum powder into 50 parts by volume of ethanol solution, performing ultrasonic extraction in an ultrasonic cleaning machine, filtering, repeating the steps for three times, combining filtrate, and concentrating the obtained filtrate to obtain a Chuzhou chrysanthemum extracting solution for later use;
(3) preparing enzymolysis pearl liquid: adding 10 volume parts of distilled water into pearl powder, adding 3 volume parts of hydrochloric acid, mixing and standing until no bubbles are generated, adding ammonia water to adjust the pH value of the solution to be neutral, heating for 40min in a water bath environment, adding papain, continuing to heat in the water bath, keeping the temperature, heating the solution to 100 ℃, continuously boiling for a period of time, cooling, fixing the volume, and refrigerating for 50 hours in a refrigerator to obtain an enzymolysis pearl solution for later use;
(4) preparing borneol-beta-cyclodextrin: accurately weighing beta-cyclodextrin and borneol according to a proportion, mixing the weighed beta-cyclodextrin with distilled water, dissolving in a water bath until the mixture is transparent, adding borneol, stirring and dissolving, carrying out ultrasonic treatment, refrigerating the mixed solution after the mixed ultrasonic treatment in a refrigerator for 24 hours, filtering, washing with absolute ethyl alcohol and distilled water for three times respectively, and drying to obtain a borneol-beta-cyclodextrin inclusion compound for later use;
(5) preparing eye patch liquid: mixing flos Chrysanthemi extractive solution, enzymolysis Margarita solution, Borneolum-beta-cyclodextrin clathrate, glycerol and Aloe water, stirring to obtain mixed eye patch solution, soaking disposable eye patch in the mixed eye patch solution, storing in refrigerator, and taking out when in use.
Wherein the concentration of the ethanol solution used in the step (2) is 75%, the power of ultrasonic extraction of the Chuzhou chrysanthemum by an ultrasonic cleaning machine is 220W, and the ultrasonic extraction time is 20 min; concentrating the finally obtained filtrate in the process of extracting the total flavonoids of the Chuzhou chrysanthemum according to a proportion to 50 times of the volume parts of the Chuzhou chrysanthemum powder; the hydrochloric acid added in the step (3) is a 14% hydrochloric acid solution, the water bath heating temperature is 45 ℃, the mass part of the papain added is half of that of the pearl powder, the water bath heating and heat preservation time is 5 hours after the papain added, the boiling time is 15min, and the volume of the solution is 50 times of that of the pearl powder when the solution is constant; in the step (4), the mass ratio of the borneol to the beta-cyclodextrin is 1: 4, the ultrasonic temperature is 35 ℃, the ultrasonic time is 30min, the water bath dissolution temperature is 55 ℃, the drying temperature after filtration is 40 ℃, and the drying time is 24 h; mixing the Chuzhou chrysanthemum extracting solution, the enzymolysis pearl solution, the borneol-beta-cyclodextrin inclusion compound, the glycerol and the aloe water in the step (5) according to the mass ratio of 20: 10: 1: 10, wherein the glycerol is 12.5 percent of glycerol; the eye patch liquid is used in the following mode: soaking the disposable eye patch in the mixed eye patch solution, refrigerating, and taking out for application. .
Example 2:
a preparation method of Chuzhou chrysanthemum eye patch liquid for protecting eyes and improving eyesight comprises the following steps:
(1) preprocessing Chuzhou chrysanthemum: preferably, intact Chuzhou chrysanthemum is dried in a dryer, then the dried Chuzhou chrysanthemum is placed in a grinder to be ground and ground, and the obtained powder is sieved to obtain Chuzhou chrysanthemum powder for later use;
(2) extracting Chuzhou chrysanthemum total flavonoids: adding the obtained Chuzhou chrysanthemum powder into 50 parts by volume of ethanol solution, performing ultrasonic extraction in an ultrasonic cleaning machine, filtering, repeating the steps for three times, combining filtrate, and concentrating the obtained filtrate to obtain a Chuzhou chrysanthemum extracting solution for later use;
(3) preparing enzymolysis pearl liquid: adding 10 volume parts of distilled water into pearl powder, adding 3 volume parts of hydrochloric acid, mixing and standing until no bubbles are generated, adding ammonia water to adjust the pH value of the solution to be neutral, heating for 40min in a water bath environment, adding papain, continuing to heat in the water bath, keeping the temperature, heating the solution to 100 ℃, continuously boiling for a period of time, cooling, fixing the volume, and refrigerating for 50 hours in a refrigerator to obtain an enzymolysis pearl solution for later use;
(4) preparing borneol-beta-cyclodextrin: accurately weighing beta-cyclodextrin and borneol according to a proportion, mixing the weighed beta-cyclodextrin with distilled water, dissolving in a water bath until the mixture is transparent, adding borneol, stirring and dissolving, carrying out ultrasonic treatment, refrigerating the mixed solution after the mixed ultrasonic treatment in a refrigerator for 24 hours, filtering, washing with absolute ethyl alcohol and distilled water for three times respectively, and drying to obtain a borneol-beta-cyclodextrin inclusion compound for later use;
(5) preparing eye patch liquid: mixing flos Chrysanthemi extractive solution, enzymolysis Margarita solution, Borneolum-beta-cyclodextrin clathrate, glycerol and Aloe water, stirring to obtain mixed eye patch solution, soaking disposable eye patch in the mixed eye patch solution, storing in refrigerator, and taking out when in use.
Wherein the concentration of the ethanol solution used in the step (2) is 65%, the power of ultrasonic extraction of the Chuzhou chrysanthemum by an ultrasonic cleaning machine is 300W, and the ultrasonic extraction time is 20 min; concentrating the finally obtained filtrate in the process of extracting the total flavonoids of the Chuzhou chrysanthemum according to a proportion to 50 times of the volume parts of the Chuzhou chrysanthemum powder; adding 10% hydrochloric acid solution into the step (3), heating in a water bath at 45 ℃, adding papain with the mass part of half of the pearl powder, continuing heating in the water bath for 5 hours after adding the papain, keeping the temperature for boiling for 15min, and fixing the volume of the solution to 50 times that of the pearl powder; in the step (4), the mass ratio of the borneol to the beta-cyclodextrin is 1: 2, the ultrasonic temperature is 40 ℃, the ultrasonic time is 1h, the water bath dissolution temperature is 55 ℃, the drying temperature after filtration is 40 ℃, and the drying time is 24 h; mixing the Chuzhou chrysanthemum extracting solution, the enzymolysis pearl solution, the borneol-beta-cyclodextrin inclusion compound, the glycerol and the aloe water in the step (5) according to the mass ratio of 20: 10: 1: 10, wherein the glycerol is 12.5 percent of glycerol; the eye patch liquid is used in the following mode: soaking the disposable eye patch in the mixed eye patch solution, refrigerating, and taking out for application. .
Example 3:
a preparation method of Chuzhou chrysanthemum eye patch liquid for protecting eyes and improving eyesight comprises the following steps:
(1) preprocessing Chuzhou chrysanthemum: preferably, intact Chuzhou chrysanthemum is dried in a dryer, then the dried Chuzhou chrysanthemum is placed in a grinder to be ground and ground, and the obtained powder is sieved to obtain Chuzhou chrysanthemum powder for later use;
(2) extracting Chuzhou chrysanthemum total flavonoids: adding the obtained Chuzhou chrysanthemum powder into 50 parts by volume of ethanol solution, performing ultrasonic extraction in an ultrasonic cleaning machine, filtering, repeating the steps for three times, combining filtrate, and concentrating the obtained filtrate to obtain a Chuzhou chrysanthemum extracting solution for later use;
(3) preparing enzymolysis pearl liquid: adding 10 volume parts of distilled water into pearl powder, adding 3 volume parts of hydrochloric acid, mixing and standing until no bubbles are generated, adding ammonia water to adjust the pH value of the solution to be neutral, heating for 40min in a water bath environment, adding papain, continuing to heat in the water bath, keeping the temperature, heating the solution to 100 ℃, continuously boiling for a period of time, cooling, fixing the volume, and refrigerating for 50 hours in a refrigerator to obtain an enzymolysis pearl solution for later use;
(4) preparing borneol-beta-cyclodextrin: accurately weighing beta-cyclodextrin and borneol according to a proportion, mixing the weighed beta-cyclodextrin with distilled water, dissolving in a water bath until the mixture is transparent, adding borneol, stirring and dissolving, carrying out ultrasonic treatment, refrigerating the mixed solution after the mixed ultrasonic treatment in a refrigerator for 24 hours, filtering, washing with absolute ethyl alcohol and distilled water for three times respectively, and drying to obtain a borneol-beta-cyclodextrin inclusion compound for later use;
(5) preparing eye patch liquid: mixing flos Chrysanthemi extractive solution, enzymolysis Margarita solution, Borneolum-beta-cyclodextrin clathrate, glycerol and Aloe water, stirring to obtain mixed eye patch solution, soaking disposable eye patch in the mixed eye patch solution, storing in refrigerator, and taking out when in use.
Wherein the concentration of the ethanol solution used in the step (2) is 70%, the power of ultrasonic extraction of the Chuzhou chrysanthemum by an ultrasonic cleaning machine is 200W, and the ultrasonic extraction time is 20 min; concentrating the finally obtained filtrate in the process of extracting the total flavonoids of the Chuzhou chrysanthemum according to a proportion to 50 times of the volume parts of the Chuzhou chrysanthemum powder; adding 12% hydrochloric acid solution into the step (3), heating in a water bath at 45 ℃, adding papain with the mass part of half of the pearl powder, continuing heating in the water bath for 5 hours after adding the papain, keeping the temperature for boiling for 15min, and fixing the volume of the solution to 50 times that of the pearl powder; in the step (4), the mass ratio of the borneol to the beta-cyclodextrin is 1: 6, the ultrasonic temperature is 35 ℃, the ultrasonic time is 1h, the water bath dissolution temperature is 55 ℃, the drying temperature after filtration is 40 ℃, and the drying time is 24 h; mixing the Chuzhou chrysanthemum extracting solution, the enzymolysis pearl solution, the borneol-beta-cyclodextrin inclusion compound, the glycerol and the aloe water in the step (5) according to the mass ratio of 20: 10: 1: 10, wherein the glycerol is 12.5 percent of glycerol; the eye patch liquid is used in the following mode: soaking the disposable eye patch in the mixed eye patch solution, refrigerating, and taking out for application.
It is noted that, herein, relational terms such as first and second, and the like may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus. Without further limitation, an element defined by the phrase "comprising an … …" does not exclude the presence of other identical elements in a process, method, article, or apparatus that comprises the element.
The above examples are only intended to illustrate the technical solution of the present invention, but not to limit it; although the present invention has been described in detail with reference to the foregoing embodiments, it will be understood by those of ordinary skill in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some technical features may be equivalently replaced; and such modifications or substitutions do not depart from the spirit and scope of the corresponding technical solutions of the embodiments of the present invention.
Claims (4)
1. A preparation method of Chuzhou chrysanthemum eye patch liquid for protecting eyes and improving eyesight is characterized by comprising the following steps of:
(1) preprocessing Chuzhou chrysanthemum: preferably, intact Chuzhou chrysanthemum is dried in a dryer, then the dried Chuzhou chrysanthemum is placed in a grinder to be ground and ground, and the obtained powder is sieved to obtain Chuzhou chrysanthemum powder for later use;
(2) extracting Chuzhou chrysanthemum total flavonoids: adding the obtained Chuzhou chrysanthemum powder into 50 volume parts of 70% ethanol solution, performing ultrasonic extraction for 20min in an ultrasonic cleaning machine by adopting 200W power, filtering, repeating the steps for three times, combining filtrate, and concentrating the obtained filtrate to obtain a Chuzhou chrysanthemum extracting solution for later use;
(3) preparing enzymolysis pearl liquid: adding 10 volume parts of distilled water into pearl powder, adding 3 volume parts of hydrochloric acid, mixing and standing until no bubbles are generated, adding ammonia water to adjust the pH value of the solution to be neutral, heating for 40min in a water bath environment, adding papain, continuing to heat in the water bath, keeping the temperature, heating the solution to 100 ℃, continuously boiling for a period of time, cooling, fixing the volume, and refrigerating for 50 hours in a refrigerator to obtain an enzymolysis pearl solution for later use;
(4) preparing borneol-beta-cyclodextrin: accurately weighing borneol and beta-cyclodextrin according to the mass ratio of 1: 6, mixing the weighed beta-cyclodextrin with distilled water, dissolving the mixture in a water bath at the temperature of 55 ℃ until the mixture is transparent, adding borneol, stirring and dissolving, carrying out ultrasonic treatment for 1h at the temperature of 35 ℃, refrigerating the mixed solution after the mixed ultrasonic treatment for 24h in a refrigerator, filtering, washing with absolute ethyl alcohol and distilled water for three times respectively, and drying at the temperature of 40 ℃ for 24h to obtain the borneol-beta-cyclodextrin inclusion compound for later use;
(5) preparing eye patch liquid: mixing and stirring Chuzhou chrysanthemum extracting solution, enzymolysis pearl solution, borneol-beta-cyclodextrin inclusion compound, glycerol and aloe water uniformly according to the mass ratio of 20: 10: 1: 10 to obtain eye patch liquid.
2. The preparation method of the Chuzhou chrysanthemum eye patch for protecting eyes and improving eyesight according to claim 1, which is characterized by comprising the following steps of: and concentrating the finally obtained filtrate in the process of extracting the total flavonoids of the Chuzhou chrysanthemum according to a proportion to 50 times of the volume parts of the Chuzhou chrysanthemum powder.
3. The preparation method of the Chuzhou chrysanthemum eye patch for protecting eyes and improving eyesight according to claim 1, which is characterized by comprising the following steps of: and (3) adding 12% hydrochloric acid solution into the solution in the step (3), heating in a water bath at 45 ℃, adding papain in a mass part which is half of the pearl powder, continuing heating in the water bath for 5 hours after adding the papain, keeping the temperature for boiling for 15min, and fixing the volume of the solution to 50 times that of the pearl powder.
4. The preparation method of the Chuzhou chrysanthemum eye patch for protecting eyes and improving eyesight according to claim 1, which is characterized by comprising the following steps of: the glycerol used in the step (5) is 12.5% of glycerol.
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| CN101843662A (en) * | 2010-05-28 | 2010-09-29 | 郑州市新视明科技工程有限公司 | Chinese medicinal composition for preventing and treating asthenopia |
| CN104644754A (en) * | 2015-03-05 | 2015-05-27 | 北京明士达生物科技有限责任公司 | Traditional Chinese medicine preparation for alleviating visual fatigue and application thereof |
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| CN101843662A (en) * | 2010-05-28 | 2010-09-29 | 郑州市新视明科技工程有限公司 | Chinese medicinal composition for preventing and treating asthenopia |
| CN104644754A (en) * | 2015-03-05 | 2015-05-27 | 北京明士达生物科技有限责任公司 | Traditional Chinese medicine preparation for alleviating visual fatigue and application thereof |
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