CN109125211A - A kind of anti-ageing activating essence of whitening and preparation method thereof - Google Patents
A kind of anti-ageing activating essence of whitening and preparation method thereof Download PDFInfo
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9794—Liliopsida [monocotyledons]
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- A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
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- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/60—Sugars; Derivatives thereof
- A61K8/602—Glycosides, e.g. rutin
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- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
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- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
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Abstract
The invention discloses anti-ageing activating essences of a kind of whitening and preparation method thereof.The Essence includes following components: moisturizer, acrylic acid (ester) class/C10-30 alkylol acrylamide acid esters cross-linked polymer, 2- oleoyl amido -1,3- octacosanol, Rhizoma Anemones Altaicae extract, persimmon leaf extract, contains researching advances on growth factor liposomes, decoyl salicylic acid, cholesterine polyethers -24, thickener, preservative at mulberry root bark extract, and surplus is deionized water.Each composition proportion science of the anti-ageing activating essence of whitening of the present invention, each ingredient have significant cooperative gain effect each other, can efficient moisturizing, moisturizing, nutrition is fed to skin, promotes skin metabolism, reduces color spot, effectively calm down microgroove, wrinkle, slowing down skin aging, improvement cutis laxa is sagging, and skin lightening is compact, and long-acting moisturizing, so that skin water is moistened smooth, high resilience, it is slight to promote skin year.
Description
Technical field
The invention belongs to cosmetic technical fields, and in particular to a kind of anti-ageing activating essence of whitening and preparation method thereof.
Background technique
Skin aging is a kind of natural law, other than being influenced by objective factors such as age, heredity, also by daylight, environment,
The many factors such as health influence.Especially modern life pressure is big, overtime work stay up late, for a long time to computer, smoking, sleep not
Foot, stay in air-conditioned room for a long time, drink, excess eye-using etc. becomes life normality, greatly accelerated skin aging, led to face
Cutis laxa, coarse, wrinkle increase, and skin complexion is obscure, color spot deposition, lacks gloss.
Studies have shown that skin oxidative is the biggest threat of skin aging, free radical is the central factor for causing skin aging,
Along with the overall process of first intermediary and later stages skin ageing.The infringement of free radical can destroy the structure of protein, accelerate young albumen
Passage, cause the premature ageing of skin；Free radical can destroy cell DNA, so that collagen and elastomer is followed the string, make
Skin resistance decline, wrinkle are deepened, and relaxation aggravation causes the mid-term aging of skin；Free radical can hinder the shape of mitochondrial ATP
At, mitochondria energy is insufficient, then skin can it is coarse it is obscure, wrinkle is loose, cause the later period aging of skin.
In addition, existing research is reported, other than melanin, other two classes pigment is detected in the skin and tissue of aging
Substance, respectively lipofuscin and cured sample matter, they can make skin darkening and coarse, especially in the elderly and congenital old silly
Most obvious on slow-witted disease patient skin, the formation of both substances is all tight with free radical, metal ion and lipid peroxidation process
It is close connected.Ultra-oxygen anion free radical can be generated in skin through a variety of ways, is turned under superoxide dismutase effect
Hydrogen peroxide is turned to, hydroxyl radical free radical is generated under iron or copper ion effect, attack unsaturated fatty acid takes out hydrogen and generates lipid certainly
By base, is reacted with oxygen and generate the series reactions such as rouge peroxy radical.Lipid peroxidation is easy to decompose and accumulate high reaction
Active carbonyls such as malonaldehyde, Schiff bases substance, these substances are the material bases to form lipofuscin and cured sample matter.
Currently, it is many kinds of for the anti-ageing skin-protection product of whitening on the market, but active constituent content is very low, for skin
The problem of cutis laxa caused by aging, coarse, wrinkle increase, the colour of skin is obscure, color spot deposition, shortage gloss carries out formula design
When the angle that considers it is not comprehensive enough, cannot finally make the use of skin-protection product from the reason of the formation of the comprehensive consideration above problem
Effect is not significant.In addition, the also generally existing poor permeability of existing skin care item, is not easy the problems such as absorbing.Therefore, it is necessary to provide
A kind of good penetrability is easy to absorb, and has the multi-functional anti-ageing skin care item of safely and effectively whitening, to meet the market demand.
Summary of the invention
The object of the present invention is to provide anti-ageing activating essences of a kind of whitening and preparation method thereof.The Essence contains
A variety of natural nourishing ingredients, each ingredient is mutually coordinated, by promoting cutin to fall off, Endothelin being inhibited to be proliferated melanocyte
Regulation inhibits tyrosine kinase activity to play significant whitening effect；By removing free radical, promoting skin collagen matter, elasticity
The regeneration of fiber and other vigor protein, active cell vigor play significant anti-ageing effect.
The present invention is achieved by the following technical programs:
A kind of anti-ageing activating essence of whitening, by mass percentage, including following components: moisturizer 2~6%, propylene
Sour (ester) class/C10-30 alkylol acrylamide acid esters cross-linked polymer 0.1~2%, 2- oleoyl amido -1,3- octacosanol 0.1~
2%, mulberry root bark extract 1~3%, Rhizoma Anemones Altaicae extract 1~3%, persimmon leaf extract 1~5%, lipid containing growth factor
Body 2~7%, decoyl salicylic acid 0.1~2%, cholesterine polyethers -24 1~3%, thickener 0.1~0.8%, preservative 0.1~
0.3%, surplus is deionized water.
It is preferred that the anti-ageing activating essence of the whitening, by mass percentage, including following components: moisturizer 4~
6%, acrylic acid (ester) class/C10-30 alkylol acrylamide acid esters cross-linked polymer 0.5~1.5%, 2- oleoyl amido -1,3- octadecane
Glycol 0.5~1.5%, Rhizoma Anemones Altaicae extract 1~2%, persimmon leaf extract 2~3%, contains mulberry root bark extract 1~2%
Researching advances on growth factor liposomes 3~6%, decoyl salicylic acid 0.5~1.5%, cholesterine polyethers -24 1~3%, thickener 0.2~
0.5%, preservative 0.1~0.3%, surplus are deionized water.
Described contains researching advances on growth factor liposomes according to parts by weight, includes following components: Crithmum maritimum callus tissue culture
10~20 parts of filtrate, 0.00005~0.001 part of recombinant human epidermal growth factor, α -1~3 part of glucoside aurantiamarin, hydrogenation
35~45 parts of soybean lecithin, 5~15 parts of cholesterol, 5~15 parts of cupreol and 4~8 parts of Tween-80.
It is preferred that described contains researching advances on growth factor liposomes according to parts by weight, it include following components: the training of Crithmum maritimum callus
Support 15 parts of object filtrate, 0.0001 part of recombinant human epidermal growth factor, α -2 parts of glucoside aurantiamarin, hydrogenated soy phosphatidyl choline
40 parts, 10 parts of cholesterol, 5 parts of cupreol and 5 parts of Tween-80.
The preparation containing researching advances on growth factor liposomes the following steps are included:
(1) α-glucoside aurantiamarin, hydrogenated soy phosphatidyl choline, cholesterol, cupreol and tween-of formula ratio is weighed
80, mixing is added dehydrated alcohol, dissolution is sufficiently stirred, obtains solution A；
(2) recombinant human epidermal growth factor is dissolved in the PBS buffer solution that pH is 7.2, stirring is made to being completely dissolved
At the solution of 0.1~0.2mg/mL, the Crithmum maritimum callus tissue culture filtrate of formula ratio is then added, is uniformly mixed, obtains
Solution B；
(3) in the case where magnetic stirring apparatus is stirred continuously, solution B is injected into solution A with syringe, it is sufficiently mixed 0.5~
1h, then at 28~32 DEG C, rotary evaporation under conditions of 0.1~1MPa removes ethyl alcohol, then vibrates water in 30~35 DEG C of water-baths
Change 1~2h of reaction, obtain liposome suspension, using the partial size of syringe type filter adjustment liposome suspension to 100~
150nm is to get containing researching advances on growth factor liposomes.
The moisturizer is selected from tocopherol, acetyl chitosamine, ceramide, pantethine, hydroxyproline, double third sweet
One or more of alcohol or glycerol.
The thickener is selected from xanthan gum, hydroxyethyl cellulose, card wave 940 or acrylamide dimethyltaurine
One or more of ammonium/VP copolymer.
The preservative is one or more of Phenoxyethanol or Sensiva SC50.
It is preferred that the preservative is that Phenoxyethanol and Sensiva SC50 are formed with the mass ratio of 1:1.
The present invention also provides a kind of preparation methods of above-mentioned anti-ageing activating essence of whitening comprising following steps: will
Deionized water, moisturizer, acrylic acid (ester) class/C10-30 alkylol acrylamide acid esters cross-linked polymer, 2- oleoyl amido -1,3- 18
Alkane glycol, decoyl salicylic acid, cholesterine polyethers -24 and thickener are added in reaction pot, open stirring, are heated to 75~80 DEG C,
It is dispersed with stirring uniformly；Cooling circulating water is opened, mulberry root bark extract, nine sections is added when temperature is down to 45~50 DEG C in stirring cooling
Calamus extract, persimmon leaf extract are added in reaction pot containing researching advances on growth factor liposomes and preservative, homogeneous 4~6 minutes, stir
It is complete to mix dissolution；Continue stirring to be cooled to room temperature, stops stirring to get the anti-ageing activating essence of whitening.
The anti-ageing activating essence of whitening provided by the invention is mentioned with mulberry root bark extract, Rhizoma Anemones Altaicae extract, persimmon leaves
It takes object, contain researching advances on growth factor liposomes, decoyl salicylic acid as principle active component, by promoting cutin to fall off, inhibiting Endothelin
Regulation to melanocyte proliferation inhibits tyrosine kinase activity to play significant whitening effect；By removing free radical, promoting
Into skin collagen matter, the regeneration of elastomer and other vigor protein, active cell vigor plays significant anti-ageing effect.
Mulberry root bark extract in formula prevents melanin pigmentation by inhibition tyrosine kinase activity, simultaneously because its
Rich in polyphenols, there is good inoxidizability, it is possible to reduce the anti-ageing effect of whitening is played in the injury of free radical；Nine
Rhizoma Anemones Altaicae extract itself contains a certain amount of catalase (CAT), and can enhance the work of superoxide dismutase (SOD)
Property, stablize target cell membrane, scavenging effect all had to hydroxy radical, hydrogen peroxide, singlet oxygen, can inhibit lipid peroxidation,
It reduces malonaldehyde to generate, inhibits the formation of lipofuscin and cured sample matter.Persimmon leaf extract is rich in Endothelin Receptor inhibitor black bearberry
Sour component, the ingredient can antagonism Endothelin in conjunction with the endothelin receptor on melanocyte, to inhibit Endothelin to black
The stimulation of plain cell inhibits differentiation, the proliferation of melanocyte, inhibits the formation of melanin.Decoyl salicylic acid has preferable
Exfoliating effect, can accelerate cutin to fall off, and promote the black degraded and elapsed to surface layer differentiation with epithelial cells by lysosome
Element falls off with horn cell, achievees the purpose that desalinate pigment.Therefore, above four kinds of ingredients are by inhibiting tyrosine-kinase enzyme activity
Property, scavenging hydroxyl, hydrogen peroxide and singlet oxygen, anti-lipid peroxidation inhibit Endothelin to be proliferated melanocyte
Regulation, accelerate melanin fall off with horn cell, from multi-angle, comprehensive effect, play significant whitening effect, make skin
The pale gloss of fast quick-recovery.
Recombinant human epidermal growth factor can promote Skin Cell to the absorption of nutriment, accelerate the new old generation of cell
It thanks, promotes the division and growth of Skin Cell, promote the synthesis of hyaluronic acid and glycoprotein, there is significant tender protecting skin function
Effect, and the synthesis and cell metabolism of the protein of epidermal cell, DNA, RNA can be enhanced, promote skin scavenger-cell molecule toxin
Ability, anti-ageing significant effect.Crithmum maritimum callus tissue culture filtrate, unique growing environment assign its unique life system
System, the active nutrient carried has significant free radical scavenging ability, and can act on epidermis and skin corium simultaneously, helps
The regeneration of cell protein promotes the generation and building of extracellular matrix, increases the compact degree of cell and skin, reduces wrinkle,
Allow skin more flexible smooth.α-glucoside aurantiamarin can promote blood circulation, is recycled by stimulation skin surface, promotes skin thin
Absorption of the born of the same parents to nutriment, accelerates the metabolism of cell, improves that the colour of skin is obscure and look tired.Further, by recombined human table
Liposome is made in skin cell growth factor, Crithmum maritimum callus tissue culture filtrate and α-glucoside aurantiamarin, is added to formula
It is middle carry out Essence preparation, can not only reduce α-glucoside aurantiamarin to the irritation of skin, also advantageously improve effectively at
The stability divided, promotes the Transdermal absorption of effective component.
Ingredient in inventive formulation is unless otherwise instructed commercial product.Wherein, the mulberry root bark extract (root bark of white mulberry
Extract), it is purchased from Xi'an Bioisystech Co., Ltd of Tianrui.Rhizoma Anemones Altaicae extract is purchased from the limited public affairs of Xi'an Tianrui biotechnology
Department.Persimmon leaf extract is purchased from Shaanxi Ci Yuan Bioisystech Co., Ltd.2- oleoyl amido -1,3- octacosanol CAS is
54422-45-6 is purchased from Hubei Yuancheng Saichuang Technology Co., Ltd..Decoyl salicylic acid CAS is 78418-01-6, is purchased from Xi'an
Jia Bo is full of Biotechnology Co., Ltd.Recombinant human epidermal growth factor CAS is 62253-63-8, is purchased from the safe and sound life in Shenzhen
Object Science and Technology Ltd..α-glucoside aurantiamarin is purchased from Guangzhou Bao Long International Trading Company Ltd.
The Crithmum maritimum callus tissue culture filtrate is made by following steps:
(1) first molten with 70% ethyl alcohol on superclean bench using Crithmum maritimum blade as the explant of evoked callus
Liquid impregnates 5min, then with aseptic water washing 3~5 times, blots surface moisture with sterilizing filter paper, be cut into the small of 0.3cm × 0.3cm
Block is inoculated in MS+0.8~1.2mg/L 6-BA+1.2~2mg/L 2, the solid medium of 4-D+4g/L agar+25g/L sucrose
Middle carry out callus induction, condition of culture are 25 ± 2 DEG C of temperature, light application time 12h/d, 30~40mmoLm of intensity of illumination-2·s-1, the squamous subculture time is 65~75 days, chooses the callus of continuous subculture 3 times loose ivory buffs；
(2) the loose ivory buff callus for taking step (1) to obtain, it is big to be divided into mung bean with sterilized tweezers
It is small, it is seeded to 1/2MS+1.5 containing 40mL~2mg/L 6-BA+0.5~1mg/L 2,4-D+30g/L sucrose, the liquid that pH is 5.8
In the 100mL conical flask of body culture medium, on 25~27 DEG C of constant-temperature table, 100rpm is carried out under the conditions of 12h/d light application time
Suspend culture, after cultivating 15~20d, filtrate is taken after being sieved through filter with 50 aim cells, filtrate is used 80 aim cells to be sieved through filter again, taken
Filtrate sterilizes to get Crithmum maritimum callus tissue culture filtrate.
The following component title occurred in text of the invention is INCI (International Nomenclature
Cosmetic Ingredient, i.e. international cosmetic raw material name) defined title: acrylic acid (ester) class/C10-30 alkanol
Acrylate crosspolymers, acrylamide dimethyltaurine ammonium/VP copolymer.
Compared with prior art, the invention has the following advantages:
(1) each composition proportion science of the anti-ageing activating essence of whitening of the present invention, the mulberry root bark extract contained, acorus graminens soland
Extract, persimmon leaf extract have significant collaboration containing nutritional ingredients such as researching advances on growth factor liposomes, decoyl salicylic acids each other
Gain effect, can efficiently moisturizing, moisturizing, give skin to feed nutrition, promote skin metabolism, reduce color spot, effectively calm down thin
Line, wrinkle, slowing down skin aging, improvement cutis laxa is sagging, and skin lightening is compact, and long-acting moisturizing, keeps skin water profit smooth, rich
It is slight to promote skin year for elasticity.
(2) the anti-ageing activating essence safety and stability of whitening of the present invention, property is mild, good penetrability, easily absorbs, uses rear face
Portion is not clearly sticky, and stable preparation process, quality controllable.
Specific embodiment
The following examples are further illustrations of the invention, rather than limiting the invention.
The preparation of 1 Crithmum maritimum callus tissue culture filtrate of embodiment
Crithmum maritimum callus tissue culture filtrate is made by following steps:
(1) first molten with 70% ethyl alcohol on superclean bench using Crithmum maritimum blade as the explant of evoked callus
Liquid impregnates 5min, then with aseptic water washing 5 times, blots surface moisture with sterilizing filter paper, be cut into the fritter of 0.3cm × 0.3cm, connect
Kind carries out callus in the solid medium of MS+1.0mg/L 6-BA+2mg/L 2,4-D+4g/L agar+25g/L sucrose
Induction, condition of culture are 27 DEG C of temperature, light application time 12h/d, intensity of illumination 35mmoLm-2·s-1, the squamous subculture time is
65 days, choose the callus of continuous subculture 3 times loose ivory buffs；
(2) the loose ivory buff callus for taking step (1) to obtain, it is big to be divided into mung bean with sterilized tweezers
It is small, it is seeded to the 6-BA+1mg/L of 1/2MS+2mg/L containing 40mL 2,4-D+30g/L sucrose, the fluid nutrient medium that pH is 5.8
In 100mL conical flask, on 25 DEG C of constant-temperature table, 100rpm carries out suspension culture under the conditions of 12h/d light application time, cultivates
After 20d, filtrate is taken after being sieved through filter with 50 aim cells, filtrate uses 80 aim cells to be sieved through filter again, takes filtrate, sterilizes to get sea
Fennel callus tissue culture filtrate.
Embodiment 2 is prepared containing researching advances on growth factor liposomes
Formula composition of the embodiment 2 containing researching advances on growth factor liposomes includes component described in the following table 1 according to parts by weight:
Formula composition of the table 1 containing researching advances on growth factor liposomes
Be formulated 1 preparation containing researching advances on growth factor liposomes the following steps are included:
(1) α-glucoside aurantiamarin, hydrogenated soy phosphatidyl choline, cholesterol, cupreol and tween-of formula ratio is weighed
80, mixing is added dehydrated alcohol, dissolution is sufficiently stirred, obtains solution A；
(2) recombinant human epidermal growth factor is dissolved in the PBS buffer solution that pH is 7.2, stirring is made to being completely dissolved
At the solution of 0.2mg/mL, the Crithmum maritimum callus tissue culture filtrate of formula ratio is then added, is uniformly mixed, obtains solution B；
(3) in the case where magnetic stirring apparatus is stirred continuously, solution B is injected into solution A with syringe, is sufficiently mixed 0.5h,
Then at 28 DEG C, rotary evaporation under conditions of 0.5MPa removes ethyl alcohol, then hydration reaction 2h is vibrated in 30 DEG C of water-baths, obtains rouge
Plastid suspension, using syringe type filter adjustment liposome suspension partial size to 100~150nm to get containing growth because
Sub- liposome.
It is formulated preparation step reference formulation 1 of the 2-5 containing researching advances on growth factor liposomes.
The preparation of the anti-ageing activating essence of 3 whitening of embodiment
The preparation of the anti-ageing activating essence of 3 whitening of embodiment includes the component of weight percent meter shown in the following table 2:
The formula composition of the anti-ageing activating essence of 2 whitening of table
Preparation step: by deionized water, moisturizer, acrylic acid (ester) class/C10-30 alkylol acrylamide acid esters cross-linked polymer,
2- oleoyl amido -1,3- octacosanol, decoyl salicylic acid, cholesterine polyethers -24 and thickener are added in reaction pot, and unlatching is stirred
It mixes, is heated to 80 DEG C, be dispersed with stirring uniformly；Cooling circulating water is opened, mulberry root skin is added when temperature is down to 45 DEG C in stirring cooling
Extract, persimmon leaf extract, contains in researching advances on growth factor liposomes and preservative addition reaction pot, homogeneous 5 Rhizoma Anemones Altaicae extract
Minute, stirring and dissolving is complete；Continue stirring to be cooled to room temperature, stops stirring to get the anti-ageing activating essence of whitening.
The preparation of the anti-ageing activating essence of 1 whitening of comparative example
The preparation of the anti-ageing activating essence of 1 whitening of this comparative example includes the component of weight percent meter shown in the following table 3:
The formula composition of the anti-ageing activating essence of 3 whitening of table
Preparation step reference implementation example 3.
Test example one, human skin patch
The related request of " human skin patch " in 2015 editions " cosmetics health specifications " issued referring to the Ministry of Public Health
1-5 is formulated to the embodiment of the present invention 3 with measuring method, comparative example 1 is formulated the anti-ageing activating essence of whitening made from 6-11 and carries out
Test, respectively 0h, 6h, 12h, for 24 hours, 48h observe dermoreaction, the experimental result of all trial volunteers is negative knot
Fruit does not occur skin adverse reaction.Show that the anti-ageing activating essence of whitening provided by the invention is nonirritant, it is highly-safe.
Test example two, the evaluation of Essence whitening effect
110 women trial volunteers are screened, the age 30-50 years old, average age 35 ± 5 years old, all trial volunteers were equal
Without serious systemic disease, disimmune defect or autoimmune disease person, without anaphylactia, tested period is not used various
Cosmetics etc., and defined content person can be completed by requirement of experiment, and face has since solarization, operating pressure, age etc. make
At spot.Trial volunteer is divided into 11 groups, every group of 10 people, respectively test 1-5 group and comparison 1-6 group, wherein test 1-
5 groups are formulated the anti-ageing activating essence of whitening made from 1-5 using embodiment 3, and comparison 1-6 group is formulated 6-11 system using comparative example 1
The anti-ageing activating essence of whitening obtained.
Application method: after morning and evening face cleaning skin toner, taking tested Essence appropriate, and point is applied to face, uniformly smears and massages
To absorption.Respectively before use, the skin manufactured after using 1 month with Courage+Khazaka company of Germany (German CK company)
Skin melanin MexameterMX18 test probe, tests the melanin value of facial skin, and facial test zone is place between the eyebrows middle
Up 2cm forehead region, lower lip middle left and right cheek region at 2cm chin area, nose and pupil intersection down.It takes
As a result average value see the table below shown in 4.
4 each group volunteer's dermal melanin comparision contents (%) of table
| Group | Before use | After use | The range of decrease |
| Test 1 group | 173.24 | 136.4 | -36.84 |
| Test 2 groups | 170.59 | 134.87 | -35.72 |
| Test 3 groups | 168.72 | 135.42 | -34.10 |
| Test 4 groups | 170.08 | 132.16 | -37.92 |
| Test 5 groups | 174.20 | 141.55 | -33.65 |
| Compare 1 group | 169.05 | 143.61 | -25.44 |
| Compare 2 groups | 173.56 | 151.51 | -22.05 |
| Compare 3 groups | 171.60 | 156.38 | -15.22 |
| Compare 4 groups | 167.42 | 149.16 | -18.26 |
| Compare 5 groups | 169.74 | 152.70 | -17.04 |
| Compare 6 groups | 172.86 | 162.66 | -10.20 |
The results show that trial volunteer uses embodiment 3 to be formulated Essence made from 1-5, face's melanin content respectively
It is substantially reduced, effect is better than being formulated Essence made from 6-11 using comparative example 1, and with Essence made from the formula of embodiment 31
Using effect it is best, show that Essence provided by the invention can be obviously improved the brilliant white degree of skin, have preferable whitening effect
Fruit.
By comparison 1-3 group it is found that the liposome in formula is free of Crithmum maritimum callus tissue culture filtrate or simultaneously respectively
Without directly liposome is not added in Crithmum maritimum callus tissue culture filtrate and α-glucoside aurantiamarin, or formula, influence
The reduction of skin melanin content shows the filtrate of callus tissue culture containing Crithmum maritimum, α-glucoside aurantiamarin and recombined human table
The liposome of three kinds of active constituents of skin cell growth factor has effects that reduction skin melanin content certain；By comparing 4-6
Group it is found that be free of Rhizoma Anemones Altaicae extract, persimmon leaf extract, or be free of Rhizoma Anemones Altaicae extract and persimmon simultaneously respectively in formula
Cotyledon extract, on reducing, skin melanin content influence is very big, especially worst to compare 6 groups of using effect, shows mulberry root
Bark extract, Rhizoma Anemones Altaicae extract, persimmon leaf extract, three kinds of ingredients are mutually coordinated, play optimal whitening effect.
Test example three, the evaluation of Essence anti-wrinkle effect
Screening women trial volunteer 110, the age 35-60 years old, all trial volunteer's skin healths, no skin allergy
History meets subject enrollment standard, is divided into 11 groups, every group of 10 people, respectively test 1-5 group and comparison 1-6 group, wherein test
1-5 group is formulated Essence made from 1-5 using embodiment 3, and comparison 1-6 group is formulated Essence made from 6-11 using comparative example 1.
Application method: after morning and evening face cleaning skin toner, taking tested Essence appropriate, and point is applied to face, uniformly smears and massages
To absorption.The skin elasticity CotometerMPA580 master manufactured using German Courage+Khazaka company (German CK company)
Machine, used probe are Reviscometer RV600 elastic fibrous tissue test probes, survey five times and are averaged.Test section
Domain: eye circumference skin.The blank value (R2, R5, R7) of 30min after being tested region clean is first measured, then provides that subject is daily
Sooner or later adhere to that, using above-mentioned trial target, test zone skin elasticity index R2, R5, R7 value (was tested after testing 0 week, 4 weeks respectively
It is 450mbar that negative pressure is constant in journey).
Index explanation: for skin elasticity index R2, R5, R7 closer to 1, the elasticity of skin is better.
R2=Ua/Uf；R5=Ur/Ue；R7=Ur/Uf.
Uf --- skin maximum tension amount.
Ue --- after constant negative pressure is added on skin, the amount of tension of skin, positioning elastic part amount of tension at 0.1 second.
Ur --- after cancelling negative pressure, skin will restore to the original state rapidly state, equally be divided into elastic portion score value Ur, that is, cancel negative
Pressure is after 0.1 second, the recovery value and visco-elastic portions value or plastic value of skin.
Ua --- from cancellation negative pressure to the recovery value of skin when follow-on test skin surface adds negative pressure again next time.
Simultaneously using every trial volunteer's skin of skinanalysis apparatus (Derma-Expert MC760) periodically follow-up investigation
Change of moisture content.As a result it see the table below shown in 5,6.
5 Essence of table improves skin elasticity effect assessment
The results show that trial volunteer uses embodiment 3 to be formulated made from 1-5 respectively, R2, R5, R7 are equal after Essence 4 weeks
Rise obvious, and R2, R5, R7 value level off to 1, and the skin elasticity index of subject has very big promotion, hence it is evident that better than using
Comparative example 1 is formulated Essence made from 6-11, and best with the using effect of Essence made from the formula of embodiment 31, thus may be used
See, the anti-ageing activating essence of whitening of the present invention, which has, to be increased skin elasticity, calms down microgroove, anti-aging function.
6 Essence of table improves moisture content of skin effect assessment
Note: D0The moisture content of skin measured for the 0th day, D14The 14th day moisture content of skin measured, D28It was measured for the 28th day
Moisture content of skin.
The results show that trial volunteer uses embodiment 3 to be formulated made from 1-5 respectively, skin contains after Essence 2 weeks, 4 weeks
Water is obviously improved, hence it is evident that is formulated Essence made from 6-11 better than using comparative example 1, it can be seen that, present invention beauty
White anti-ageing activating essence can efficiently moisturizing, moisturizing, effectively promote the elasticity of skin, allow skin to restore water tender smooth.
The above is only the preferred embodiment of the present invention, it is noted that above-mentioned preferred embodiment is not construed as pair
Limitation of the invention, protection scope of the present invention should be defined by the scope defined by the claims..For the art
For those of ordinary skill, without departing from the spirit and scope of the present invention, several improvements and modifications can also be made, these change
It also should be regarded as protection scope of the present invention into retouching.
Claims (9)
1. a kind of anti-ageing activating essence of whitening, which is characterized in that by mass percentage, including following components: moisturizer 2~
6%, acrylic acid (ester) class/C10-30 alkylol acrylamide acid esters cross-linked polymer 0.1~2%, 2- oleoyl amido -1,3- octadecane two
Alcohol 0.1~2%, Rhizoma Anemones Altaicae extract 1~3%, persimmon leaf extract 1~5%, contains growth at mulberry root bark extract 1~3%
Factor liposome 2~7%, cholesterine polyethers -24 1~3%, thickener 0.1~0.8%, is prevented decoyl salicylic acid 0.1~2%
Rotten agent 0.1~0.3%, surplus are deionized water.
2. the anti-ageing activating essence of whitening according to claim 1, which is characterized in that by mass percentage, including with
Lower component: moisturizer 4~6%, acrylic acid (ester) class/C10-30 alkylol acrylamide acid esters cross-linked polymer 0.5~1.5%, 2- oil
Amide groups -1,3- octacosanol 0.5~1.5%, mulberry root bark extract 1~2%, Rhizoma Anemones Altaicae extract 1~2%, persimmon
Leaf extract 2~3%, containing researching advances on growth factor liposomes 3~6%, decoyl salicylic acid 0.5~1.5%, cholesterine polyethers -24 1~
3%, thickener 0.2~0.5%, preservative 0.1~0.3%, surplus are deionized water.
3. the anti-ageing activating essence of whitening according to claim 1 or 2, which is characterized in that the rouge containing growth factor
Plastid includes following components: 10~20 parts of Crithmum maritimum callus tissue culture filtrate, recombinant human epidermal cell according to parts by weight
0.00005~0.001 part of growth factor, α -1~3 part of glucoside aurantiamarin, 35~45 parts of hydrogenated soy phosphatidyl choline, cholesterol 5
~15 parts, 5~15 parts of cupreol and 4~8 parts of Tween-80.
4. the anti-ageing activating essence of whitening according to claim 3, which is characterized in that described contains researching advances on growth factor liposomes
It according to parts by weight, include following components: 15 parts of Crithmum maritimum callus tissue culture filtrate, recombinant human epidermal growth factor
0.0001 part, α -2 parts of glucoside aurantiamarin, 40 parts of hydrogenated soy phosphatidyl choline, 10 parts of cholesterol, 5 parts of cupreol and Tween-80
5 parts.
5. the anti-ageing activating essence of whitening according to claim 3, which is characterized in that described contains researching advances on growth factor liposomes
Preparation the following steps are included:
(1) α-glucoside aurantiamarin, hydrogenated soy phosphatidyl choline, cholesterol, cupreol and Tween-80 of formula ratio is weighed, is mixed
It closes, dehydrated alcohol is added, dissolution is sufficiently stirred, obtains solution A；
(2) recombinant human epidermal growth factor is dissolved in the PBS buffer solution that pH is 7.2, stirs to being completely dissolved, is made
Then the Crithmum maritimum callus tissue culture filtrate of formula ratio is added in the solution of 0.1~0.2mg/mL, be uniformly mixed, obtain molten
Liquid B；
(3) in the case where magnetic stirring apparatus is stirred continuously, solution B is injected into solution A with syringe, is sufficiently mixed 0.5~1h, so
Afterwards at 28~32 DEG C, rotary evaporation under conditions of 0.1~1MPa removes ethyl alcohol, then oscillation aquation is anti-in 30~35 DEG C of water-baths
1~2h is answered, liposome suspension is obtained, using the partial size of syringe type filter adjustment liposome suspension to 100~150nm,
Up to containing researching advances on growth factor liposomes.
6. the anti-ageing activating essence of whitening according to claim 1, which is characterized in that the moisturizer is selected from fertility
One or more of phenol, acetyl chitosamine, ceramide, pantethine, hydroxyproline, dipropylene glycol or glycerol.
7. the anti-ageing activating essence of whitening according to claim 1, which is characterized in that the thickener is selected from xanthan
One or more of glue, hydroxyethyl cellulose, card wave 940 or acrylamide dimethyltaurine ammonium/VP copolymer.
8. the anti-ageing activating essence of whitening according to claim 1, which is characterized in that the preservative is Phenoxyethanol
Or one or more of Sensiva SC50.
9. a kind of method for preparing the anti-ageing activating essence of whitening described in claim 1, which is characterized in that including following step
It is rapid:
By deionized water, moisturizer, acrylic acid (ester) class/C10-30 alkylol acrylamide acid esters cross-linked polymer, 2- oleoyl amido -1,
3- octacosanol, decoyl salicylic acid, cholesterine polyethers -24 and thickener are added in reaction pot, open stirring, it is heated to 75~
It 80 DEG C, is dispersed with stirring uniformly；Cooling circulating water is opened, stirring cooling is added mulberry root skin and extracts when temperature is down to 45~50 DEG C
Object, persimmon leaf extract, contains in researching advances on growth factor liposomes and preservative addition reaction pot, homogeneous 4~6 Rhizoma Anemones Altaicae extract
Minute, stirring and dissolving is complete；Continue stirring to be cooled to room temperature, stops stirring to get the anti-ageing activating essence of whitening.
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| CN111317701A (en) * | 2020-04-02 | 2020-06-23 | 广州一一生物技术有限公司 | Skin barrier repair compound, skin care emulsion and preparation method thereof |
| CN112263540A (en) * | 2020-11-02 | 2021-01-26 | 浙江英树生物科技有限公司 | Whitening composition and preparation method and application thereof |
| CN113893199A (en) * | 2021-11-12 | 2022-01-07 | 湖北省麦诗特生物科技有限公司 | A facial cream composition with skin thickness increasing effect and its preparation method |
| CN115887322A (en) * | 2022-11-29 | 2023-04-04 | 北京植物医生生物科技有限公司 | Essence capable of whitening, removing freckles, smoothing skin and preserving moisture and preparation method thereof |
| CN115887322B (en) * | 2022-11-29 | 2024-06-07 | 北京植物医生生物科技有限公司 | Whitening and freckle removing, skin smoothing and moisturizing essence and preparation method thereof |
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| CN115887322B (en) * | 2022-11-29 | 2024-06-07 | 北京植物医生生物科技有限公司 | Whitening and freckle removing, skin smoothing and moisturizing essence and preparation method thereof |
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