CN109124809B - Neonatal suckling mouse myocardial infarction model puncture outfit - Google Patents
Neonatal suckling mouse myocardial infarction model puncture outfit Download PDFInfo
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- CN109124809B CN109124809B CN201811094409.9A CN201811094409A CN109124809B CN 109124809 B CN109124809 B CN 109124809B CN 201811094409 A CN201811094409 A CN 201811094409A CN 109124809 B CN109124809 B CN 109124809B
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61D—VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
- A61D1/00—Surgical instruments for veterinary use
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Abstract
The invention provides a neonatal rat myocardial infarction model puncture outfit, a puncture device and a use method thereof, and further provides a hoof-shaped clamp with special shape, which is arranged at one end of a handle of the neonatal rat myocardial infarction model puncture outfit, and because other special shapes are completely attached to the arc-shaped surface of cardiac muscle, the neonatal rat myocardial infarction model puncture outfit can form a ladle to the heart, thereby solving the possibility of left and right shaking of the heart during puncture and avoiding injuring cardiac muscle; furthermore, the spring in the puncture outfit utilizes elastic potential energy to penetrate the puncture needle through the preset heart tissue, so that the puncture operation can be completed rapidly, the time is short, the efficiency is high, the experimental process is accelerated, the experimental time is shortened, and the operation survival rate is improved.
Description
Technical Field
The invention relates to a newborn suckling mouse myocardial infarction model puncture outfit, in particular to a puncture device with a puncture outfit, which comprises a hoof-shaped clamp with a special shape in one step, and belongs to the field of medical appliances.
Background
In medical research, the newborn suckling mice have a fast heart rate and a small heart volume, so that the myocardial infarction model of the newborn suckling mice is rarely researched at present. In the manufacturing of myocardial infarction model, all accomplish through manual operation at present, there are two major problems at present: on the one hand, as the heart rate of the suckling mice is high, the frequency of each minute reaches 500-600 times, and the quick contraction and relaxation dynamic state is presented, so that an experimenter can shake easily during model making, the puncture position is not fixed, the ligation position of an myocardial infarction model is influenced, and experimental data is inaccurate. On the other hand, as the heart rate of the suckling mice is high, the puncture needle insertion time is long, the operation time is prolonged, and the death rate of the experimental object is increased.
Disclosure of Invention
The invention aims to solve the technical problem of overcoming the defects of the prior art and providing a neonatal suckling mouse myocardial infarction model needle penetrating device.
In order to solve the technical problems, the invention adopts the following technical scheme:
the puncture outfit of the neonatal suckling mouse myocardial infarction model comprises a member with a groove in the middle, a puncture needle transmitting system is arranged at the left part of the member, and a puncture needle receiving system is arranged at the right part of the member; the inner surface of the groove is attached to the outer surface of the heart of the suckling mouse.
Further, the launching system comprises a launching spring, a push pin connecting rod and a needle storage cavity, one end of the launching spring is clung to one end of the push pin connecting rod, the other end of the push pin connecting rod stretches into the needle storage cavity, and after the launching spring is released, the push pin connecting rod pushes a puncture needle in the needle storage cavity to be ejected outwards.
Further, the receiving system comprises a receiving cavity, an upper receiving connecting rod, a lower receiving connecting rod, an upper spring and a lower spring, wherein one end of the upper spring is clung to one end of the upper receiving connecting rod, the other end of the upper receiving connecting rod is arranged in the receiving cavity, one end of the lower spring is clung to one end of the lower receiving connecting rod, the other end of the lower receiving connecting rod is arranged in the receiving cavity, and the upper spring and the lower spring are released to respectively push the upper receiving connecting rod and the lower receiving connecting rod to move so as to clamp a puncture needle ejected from the needle storage cavity.
Further, the lifting operation rod, the release launching spring steel wire and the pull-back launching spring steel wire are included, the release launching spring handle and the pull-back launching spring handle are released, the receiving spring steel wire and the pull-back receiving spring steel wire are released, and the receiving spring handle and the pull-back receiving spring handle are released; one end of the release launching spring steel wire penetrates through the lifting operation rod to be connected with the launching spring, the other end of the release launching spring steel wire is connected with the release launching spring handle, one end of the pull-back launching spring steel wire penetrates through the lifting operation rod to be connected with the launching spring, and the other end of the pull-back launching spring steel wire is connected with the pull-back launching spring handle.
Further, one end of the release receiving spring steel wire passes through the lifting operation rod to be connected with the upper spring and the lower spring, the other end of the release receiving spring steel wire is connected with the release receiving spring handle, one end of the pull-back receiving spring steel wire passes through the lifting operation rod to be connected with the upper spring and the lower spring, and the other end of the pull-back receiving spring steel wire is connected with the pull-back receiving spring handle.
Further, the puncture needle comprises a ligature and a thread storage chamber, wherein the ligature is arranged in the thread storage chamber, and one end of the ligature is connected with the puncture needle.
In another aspect of the present invention, there is provided a puncture device comprising the above puncture outfit, further comprising a handle, the liftable operating rod being disposed at an intersection point of the handle;
the hoof-shaped clamp consists of a left hoof-shaped clamp and a right hoof-shaped clamp, and is respectively arranged at one end of the handle and used for forming a ladle to the heart.
Further, the left hoof-shaped clamp and the right hoof-shaped clamp have the same structure and are composed of steel wires similar to an ellipse and similar to a trapezoid, and the left hoof-shaped clamp and the right hoof-shaped clamp are characterized in that the whole arc ring similar to the ellipse is in a ton shape, and the central line similar to the trapezoid is perpendicular to the long axis of the similar ellipse and passes through the center point similar to the ellipse; the upper bottom of the trapezoid is left blank, and two ends of the trapezoid are respectively connected with the ellipse in a circular arc transition mode; the lower bottom similar to the trapezoid is left empty and is respectively connected with two ends of other devices; the front surface of the trapezoid is concave downwards to form an arc surface.
Further, the left and right hoof-shaped clamps are made of steel wires.
Further, the cambered surface is a cambered surface with the arc length of 1.466cm, which is caused by taking a 60-degree central angle and a radius of 1.4cm as standards.
Further, the ellipse-like major axis is about 1cm and the minor axis is about 0.8cm; the trapezoid-like upper base is about 0.4cm, the lower base is about 0.8cm, and the height is about 1.2cm.
In another aspect of the present invention, there is provided a method of using the above puncture device, comprising the steps of: the handle is operated to enable the left hoof-shaped clamp and the right hoof-shaped clamp to be inserted obliquely from the left side and the right side of the heart respectively, so that a package is formed on the heart, and the possibility of left and right shaking of the heart during puncturing is solved.
Further, the lifting operation rod is lowered, so that the inner surface of the groove of the puncture outfit is clung to the outside of the heart, and the possibility of up-and-down fluctuation of the heart is ensured.
Further, the trigger spring handle is pulled and released, the elastic potential energy of the trigger spring is released, the push needle connecting rod is pushed instantly, the push needle connecting rod transmits kinetic energy to the puncture needle, and the puncture needle obtains kinetic energy to pass through the preset heart tissue and then enter the receiving cavity.
Further, the receiving spring handle is pulled and released, the upper spring and the lower spring are released instantaneously, and the upper receiving connecting rod and the lower receiving connecting rod are pushed and pushed to move, so that the puncture needle ejected from the needle storage cavity is clamped.
Further, the liftable operating rod is lifted, the receiving spring handle is pulled back, the puncture needle with the ligature is taken out, and the ligature is performed in the next step.
The invention has the beneficial effects that:
the special-shaped hoof-shaped clamp is arranged at one end of the handle of the neonatal suckling mouse myocardial infarction model puncture outfit, and because other special shapes are completely attached to the arc-shaped surface of the cardiac muscle, the clamp forms a ladle to the cardiac, the possibility of left and right shaking of the cardiac muscle during puncture is solved, the cardiac muscle is prevented from being hurt, the puncture position of each suckling mouse is ensured to be fixed, and the consistency and the accuracy of data are ensured; furthermore, the spring in the puncture outfit utilizes elastic potential energy to penetrate the puncture needle through the preset heart tissue, so that the puncture operation can be completed rapidly, the whole puncture process is less than 2 seconds, the time is short, the efficiency is high, the experimental process is accelerated, the experimental time is shortened, and the operation survival rate is improved.
Drawings
FIG. 1 is a schematic view of the whole structure of the puncturing device of the present invention;
FIG. 2 is a schematic view of the puncture outfit according to the present invention;
fig. 3 is a schematic view of the structure of the shoe clamp of the present invention.
Fig. 4 is a schematic view showing the structure of the receiving system of the puncture needle according to the present invention.
Fig. 5 is a schematic view showing the structure of the firing system for the lancet according to the present invention.
Fig. 6 is a schematic structural view of the lifting operation rod of the present invention.
Detailed Description
The present invention will be described in detail below with reference to the accompanying drawings. It should be noted that these drawings and the written description are not intended to limit the scope of the inventive concept in any way, but to illustrate the inventive concept to those skilled in the art by referring to the specific embodiments.
1-6, in the invention, a puncture outfit 1 of a neonatal suckling mouse myocardial infarction model is provided, wherein the puncture outfit comprises a member 2 with a groove 2-1 at the middle part, a transmitting system 3 with a puncture needle 6 at the left part and a receiving system 4 with a puncture needle at the right part; the inner surface of the groove 2-1 is attached to the outer surface of the suckling mouse heart 5.
Referring to fig. 5, the launching system 3 includes a launching spring 3-1, a push pin connecting rod 3-2 and a needle storage cavity 3-3, one end of the launching spring 3-1 is tightly attached to one end of the push pin connecting rod 3-2, the other end of the push pin connecting rod 3-2 extends into the needle storage cavity 3-3, and after the launching spring 3-1 is released, the push pin connecting rod pushes the puncture needle 6 in the needle storage cavity 3-3 to be ejected outwards. The launching system 3 is provided with a pair of release spring buckles 3-4, the buckles are clamped at two sides of the proximal needle end of the launching spring 3-1, and the tail end of the buckle is connected with a release spring steel wire; the buckle 3-4 is close to one side of the spring and is connected with the triangular bracket 3-5, and the pull-back spring buckle 3-6 is connected with the spring receiving steel wire; .
Referring to fig. 4, the receiving system 4 includes a receiving cavity 4-1, an upper receiving connecting rod 4-2, a lower receiving connecting rod 4-3, an upper spring 4-4, and a lower spring 4-5, wherein one end of the upper spring 4-4 is tightly attached to one end of the upper receiving connecting rod 4-2, the other end of the upper receiving connecting rod 4-2 is disposed in the receiving cavity 4-1, one end of the lower spring 4-5 is tightly attached to one end of the lower receiving connecting rod 4-3, the other end of the lower receiving connecting rod 4-3 is disposed in the receiving cavity 4-1, and the upper spring 4-2 and the lower receiving connecting rod 4-3 are respectively pushed by the upper spring 4-5 to move, so as to clamp the puncture needle 6 ejected from the needle storage cavity. Two pairs of release spring buckles 4-9 and 4-8 pull-back spring buckles are arranged in the receiving system 4; in addition, the pull-back release steel wire is turned by a plurality of fixed pulleys 4-7 at each turning point
Further, with reference to fig. 1 to 5, the lifting device comprises a lifting operation rod 7, a release launching spring steel wire 7-1, a pull-back launching spring steel wire 7-2, a release launching spring handle 7-3, a pull-back launching spring handle 7-4, a release receiving spring steel wire 7-5, a pull-back receiving spring steel wire 7-6, a release receiving spring handle 7-7 and a pull-back receiving spring handle 7-8; one end of the release launching spring steel wire 7-1 penetrates through the lifting operation rod 7 to be connected with the launching spring, the other end of the release launching spring steel wire is connected with the release launching spring handle 7-3, one end of the pull-back launching spring steel wire 7-2 penetrates through the lifting operation rod 7 to be connected with the launching spring 3-1, and the other end of the pull-back launching spring steel wire 7-2 is connected with the pull-back launching spring handle 7-4.
Further, referring to fig. 1 to 5, one end of the release receiving spring wire 7-5 is connected to the upper spring 4-4 and the lower spring 4-5 through the liftable operating lever 7, the other end of the release receiving spring wire 7-5 is connected to the release receiving spring handle 7-7, one end of the pull-back receiving spring wire 7-6 is connected to the upper spring 4-4 and the lower spring 4-5 through the liftable operating lever 7, and the other end of the pull-back receiving spring wire 7-6 is connected to the pull-back receiving spring handle 7-8.
Referring to fig. 6, the liftable operating rod 7 vertically passes through the central shaft 8-1 of the operating handle 8, and the liftable operating rod 7 is provided with fixing holes 8-2 of 0.2 x 0.4mm on the side surface and vertically arranged for inserting one end of the L-shaped clamping connecting rod 8-3 to fix the liftable operating rod 7 in a lifting manner. One side of the long handle of the L-shaped clamping connecting rod penetrates through the fixed shaft, and the middle of the bottom surface of the long handle is connected with a spring 8-5 fixed on the center shaft, so that the clamping connecting rod 8-3 can be abutted to the center shaft 8-1. The side surface of the liftable operating rod 7 is provided with one lifting buckle 8-4, so that the lifting of the liftable operating rod is facilitated.
With reference to fig. 1-5, the ligature comprises a ligature 3-4 and a thread storing chamber 3-5, wherein the ligature 3-4 is arranged in the thread storing chamber 3-5, and one end of the ligature is connected with a puncture needle 6.
1-5, according to another aspect of the present invention, there is provided a puncture device Z comprising the puncture outfit, further comprising a handle 8, wherein the lifting operation rod 7 is disposed at the intersection point of the handle 8;
the hoof-shaped clamp consists of a left hoof-shaped clamp 8-1 and a right hoof-shaped clamp 8-2, and is respectively arranged at one end of the handle and used for forming a ladle to the heart.
With reference to fig. 3, the left hoof-shaped clamp 8-1 and the right hoof-shaped clamp 8-2 have the same structure and are composed of steel wires similar to an ellipse and similar to a trapezoid, and are characterized in that the whole arc ring similar to the ellipse is in a shape of a ton, and the center line similar to the trapezoid is perpendicular to the long axis of the similar ellipse and passes through the center point similar to the ellipse; the upper bottom of the trapezoid is left blank, and two ends of the trapezoid are respectively connected with the ellipse in a circular arc transition mode; the lower bottom similar to the trapezoid is left empty and is respectively connected with two ends of other devices; the trapezoid-like front surface is concave downwards to form a cambered surface left-shoe clamp and a cambered surface right-shoe clamp which are both made of steel wires or special materials, such as high polymer materials, other inorganic materials and the like.
Referring to fig. 3, the arc surface is an arc surface with the arc length of 1.466cm, which is based on the 60-degree central angle a and the radius r of 1.4 cm.
Referring to fig. 3, the major axis a of the ellipse is about 1cm, and the minor axis B is about 0.8cm; the trapezoid-like upper bottom C is about 0.4cm, the lower bottom D is about 0.8cm, and the height H is about 1.2cm.
In another aspect of the present invention, in connection with fig. 1-6, a method of using the above-described lancing device Z is provided, comprising the steps of: the handle 8 is operated to enable the left hoof-shaped clamp 8-1 and the right hoof-shaped clamp 8-2 to be respectively inserted obliquely from the left side and the right side of the heart 5, so that the heart 5 is wrapped, and the possibility of left and right shaking of the heart 5 during puncturing is solved. The lifting operation rod 7 is lowered, so that the inner surface of the groove 2-1 of the puncture outfit 1 is clung to the outer surface of the heart, and the possibility of up-and-down fluctuation of the heart 5 is ensured. Pulling and releasing the launching spring handle 7-3, releasing the elastic potential energy of the launching spring 3-1, pushing the push needle connecting rod 3-2 instantly, transmitting kinetic energy to the puncture needle 6 by the push needle connecting rod 3-2, and enabling the puncture needle 6 to obtain the kinetic energy to pass through the preset heart 5 tissue and enter the receiving cavity.
The lifting and releasing receiving spring handle 7-7, the upper spring 4-4 and the lower spring 4-5 are released instantaneously, the upper receiving connecting rod 4-2 and the lower receiving connecting rod 4-3 are pushed to move, and then the puncture needle 6 ejected from the needle storage cavity 3-3 is clamped.
The lifting operation rod 7 is lifted, the receiving spring handle is pulled back, the puncture needle 6 with the ligature is taken out, and the ligature is carried out next time.
The foregoing description of the preferred embodiments of the invention is not intended to be limiting, but rather to enable any modification, equivalent replacement, improvement or the like to be made within the spirit and principles of the invention.
Claims (10)
1. The puncture outfit of the neonatal suckling mouse myocardial infarction model is characterized by comprising a member with a groove in the middle, wherein a puncture needle transmitting system is arranged at the left part of the member, and a puncture needle receiving system is arranged at the right part of the member; the inner surface of recess is laminated mutually with the surface of suckling mouse heart, receiving system is including receiving the chamber, go up receiving the connecting rod, lower receiving the connecting rod, go up spring, lower spring, go up the one end of spring and go up the one end of receiving the connecting rod and hug closely, go up the other end of receiving the connecting rod and place the receiving intracavity, the one end of lower spring is hugged closely with the one end of receiving the connecting rod down, the receiving intracavity is placed in to the other end of receiving the connecting rod down, receive the connecting rod and receive the connecting rod motion down in the release is gone up to spring and lower spring respectively, and then press from both sides tightly the pjncture needle that jets out from storing needle intracavity, there are two pairs of release spring buckle in receiving system, the spring buckle of pullback 4-8.
2. The puncture outfit according to claim 1, wherein the launching system comprises a launching spring, a push pin connecting rod and a needle storage cavity, one end of the launching spring is clung to one end of the push pin connecting rod, the other end of the push pin connecting rod stretches into the needle storage cavity, and after the launching spring is released, the push pin connecting rod pushes the puncture needle in the needle storage cavity to be ejected outwards.
3. The puncture outfit according to claim 1, further comprising a lifting operation rod, a release firing spring wire, a pull-back firing spring wire, a release firing spring handle, a pull-back firing spring handle, a release receiving spring wire, a pull-back receiving spring wire, a release receiving spring handle, a pull-back receiving spring handle; one end of the release launching spring steel wire penetrates through the lifting operation rod to be connected with the launching spring, the other end of the release launching spring steel wire is connected with the release launching spring handle, one end of the pull-back launching spring steel wire penetrates through the lifting operation rod to be connected with the launching spring, and the other end of the pull-back launching spring steel wire is connected with the pull-back launching spring handle.
4. The puncture outfit according to claim 3, wherein one end of the release receiving spring wire is connected to the upper spring and the lower spring through the liftable operating rod, the other end of the release receiving spring wire is connected to the release receiving spring handle, one end of the pull-back receiving spring wire is connected to the upper spring and the lower spring through the liftable operating rod, and the other end of the pull-back receiving spring wire is connected to the pull-back receiving spring handle.
5. The puncture outfit according to claim 1 or 2 or 3 or 4, further comprising a ligature and a thread storage chamber, wherein the ligature is placed in the thread storage chamber, and one end of the ligature is connected with the puncture needle.
6. A lancing device comprising the lancing device of claims 1-5, further comprising:
the lifting operation rod is arranged at the intersection point of the handles;
the hoof-shaped clamp consists of a left hoof-shaped clamp and a right hoof-shaped clamp, and is respectively arranged at one end of the handle and used for forming a ladle to the heart.
7. The lancing device of claim 6, wherein the left and right hoops are identical in construction and each consist of oval-like and trapezoid-like steel wires, wherein the entire oval-like loop is in a ton-like shape, the centerline of the trapezoid-like being perpendicular to the major axis of the oval-like shape and passing through the center point of the oval-like shape; the upper bottom of the trapezoid is left blank, and two ends of the trapezoid are respectively connected with the ellipse in a circular arc transition mode; the lower bottom similar to the trapezoid is left empty and is respectively connected with two ends of other devices; the front surface of the trapezoid is concave downwards to form an arc surface.
8. The lancing device of claim 7, wherein the left and right hoops are each formed from steel wire.
9. The lancing device of claim 8, wherein the arc surface is an arc surface having an arc length of 1.466cm based on a 60 ° central angle and a radius of 1.4 cm.
10. The lancing device of any one of claims 7-9, wherein the elliptical shape has a major axis of about 1cm and a minor axis of about 0.8cm; the trapezoid-like upper base is about 0.4cm, the lower base is about 0.8cm, and the height is about 1.2cm.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811094409.9A CN109124809B (en) | 2018-09-19 | 2018-09-19 | Neonatal suckling mouse myocardial infarction model puncture outfit |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811094409.9A CN109124809B (en) | 2018-09-19 | 2018-09-19 | Neonatal suckling mouse myocardial infarction model puncture outfit |
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| CN109124809A CN109124809A (en) | 2019-01-04 |
| CN109124809B true CN109124809B (en) | 2023-09-01 |
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| CN109124809A (en) | 2019-01-04 |
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