CN103735277A - Heart puncture blood collecting device and puncture blood collecting method thereof for mice of medical research experiments - Google Patents
Heart puncture blood collecting device and puncture blood collecting method thereof for mice of medical research experiments Download PDFInfo
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- CN103735277A CN103735277A CN201410013716.5A CN201410013716A CN103735277A CN 103735277 A CN103735277 A CN 103735277A CN 201410013716 A CN201410013716 A CN 201410013716A CN 103735277 A CN103735277 A CN 103735277A
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Abstract
The invention discloses a heart puncture blood collecting device and a puncture blood collecting method thereof for mice of medical research experiments. The device comprises an operation plate, an anesthesia cup and a blood collecting needle, wherein the operation plate is an oblong flat plate; the middle part of the flat plate is provided with a concave groove; the front end of the concave groove is provided with a clamping groove; the interior of the clamping groove is provided with an anesthesia small tube; the left side of the concave groove is provided with a neck binding band; the back side of the neck binding band is provided with a breast binding band; the upper end of the neck binding band is provided with a neck binding upper touch and close fastener; the right side of the concave groove is provided with a neck binding lower touch and close fastener; the upper end of the breast binding band is provided with a breast binding upper touch and close fastener; the right side of the concave groove is provided with a breast binding lower touch and close fastener. The device has the advantages that the structure is simple, the design is skillful, the heart positioning is accurate, and the operation is easy, convenient and quick.
Description
Technical field
The present invention relates to a kind of medical research equipment, the definite heart puncturing extracting blood device that says a kind of experimental mouse.
Background technology
In medical research, the conventional Mus of people is made disease model, observes the medicine kinetics of metabolism or the effect of a certain Therapeutic Method of research evaluation in animal body.Show according to statistics, the scientific research problem of Medical Biology is more than half to be completed with muroid animal.Mus is as the maximum laboratory animal of quantity in medical research, and how taking a blood sample, to become of scientific research be the most substantially, also most important preorder step.Current existing blood-sampling method mostly adopts and cuts tail blood sampling, the blood sampling of eye socket rear vein beard, breaks end and get blood, sublingual vein blood sampling, vena dorsalis penis blood sampling etc., these methods not only can not meet the requirement that detects aequum because blood sampling volume is little, and easily cause haemolysis to affect the mensuration of index of correlation.As obtained more blood, have to adopt exoculation ball or disconnected neck to put to death the method for animal.So not only needing to sacrifice a large amount of experimental mouse increases experiment fees, nor meets animal welfare method.In some medical experiments usually need to same laboratory animal with it continuous several times get blood and study some substance metabolismes or the mechanism of action.
As want continuous several times to obtain a large amount of blood with it in laboratory animal, heart puncturing extracting blood generally can meet repeatedly the requirement of continuous blood sampling.Traditional heart puncturing extracting blood operating procedure is: by snarl respectively the extremity of rat after Mus anesthesia with 4 rubber bands, form slip-knot, and be fixed on such rat health maintenance extended state on 4 iron nails.Executing patient holds No. 7 needle applicators and by place, rat xiphoid-process lower edge center, tiltedly punctures into thoracic cavity (needle point becomes 20 °～35 ° with skin, if rat body weight is slightly large, needle angle is also slightly large), when needle point thrusts heart, blood is because heartbeat pours in syringe naturally, then the slow nook closing member of pull syringe.But traditional heart puncturing extracting blood has following deficiency: (1) mouse anesthesia is too shallow gets in the process of blood and easily revive, but therefore blood taking needle also understands grievous injury heart in heart, if the bradycardia of the too dark Mus of anesthesia, get blood difficulty or amount for taking blood few, even occur the phenomenon that anesthesia is excessively dead; (2) Mus lies on the back on plank, with four rubber bands, fixedly there will be the pulling force of each rubber band different, and Mus is under undernatured state, and skew appears in cardiac position, is unfavorable for finding accurately heart; The use of (3) No. 7 syringe needles, because syringe needle is long, easily punctures other organs of internal organs Mus is caused to larger injury; (4) cardiac position is difficult for determining, abecedarian need to practise finding the accurate location of heart for a long time; (5) dynamics of inserting needle, the degree of depth are wayward, and excessive heart and other organs worn out of dynamics, can not thrust heart when dynamics is inadequate; (6) while drawing blood, syringe will keep constant motionless slow blood drawing, needs operator will have very strong control force and willpower, and operation easier is large.(7) take out the Mus mortality rate of blood high, can not do follow-up scientific research.
Summary of the invention
Therefore, people are to simple in structure, and maneuverable heart puncturing extracting blood device exists great demand, also do not find about relevant report of the present invention up to now.
Object of the present invention overcomes above-mentioned defect exactly, and a kind of economy is provided, simple, maneuverable muroid heart puncturing extracting blood device, and to improve the application function with standard utensil, convenient operation, just can carry out heart puncturing extracting blood fast without a large amount of exercises.
The solution of the present invention comprises operation panel, anesthesia cup and blood taking needle, described operation panel is rectangular flat board, and dull and stereotyped middle part is provided with groove, and the front end of groove is provided with draw-in groove, anesthesia tubule is housed in draw-in groove, the left side of groove is provided with bundle neck band, and the rear side of bundle neck band is provided with bundle pectoral girdle, and the upper end of bundle neck band is provided with thread gluing on bundle neck, the right side of groove is provided with thread gluing under bundle neck, the upper end of bundle pectoral girdle is provided with thread gluing on bundle breast, and the right side of groove is provided with thread gluing under bundle breast, and the right side of operation panel is furnished with anesthesia cup.Described anesthesia cup is for the anesthesia in early stage of experimental mouse, anesthesia cup is comprised of cup and base, in cup, be provided with anesthetic room, the bottom of anesthetic room is provided with ventilative separation net, in base, be provided with anesthetis apotheca, the upper end of base is provided with female thread, and the downside of cup is provided with external screw thread, cup is arranged on the upper end of base by being threaded, the upper end of cup is provided with bowl cover.Described blood taking needle is a special special blood-drawing pin, the length of syringe needle is after experimental mouse is fixed, the thickness of heart epithelium adds heart to the length of epidermis distance, through accurate Calculation, design and produce, with avoid syringe needle because of too short puncture not in place, or because syringe needle is long, in piercing process, puncture other internal organs tissues, the needle handle of syringe needle upper end is provided with fixing hole, the fixed leg during so that take a blood sample, needle handle being articulated on bundle pectoral girdle fixes syringe needle, the upper end of syringe needle is provided with connecting hose, syringe needle is connected with syringe by connecting hose, when avoiding taking a blood sample, because the shake of hands causes rocking of syringe needle, described anesthesia tubule is the anesthesia tubule while taking a blood sample operation for experimental mouse, one end sealing of tubule, in tubule, put into and be soaked with narcotic absorbent cotton, the nose that is placed on Mus in getting blood process is sucked by Mus, make it continue to remain on narcotism.
The present invention is simple in structure, design ingenious, heart accurate positioning, easy and simple to handle, quick.
Below in conjunction with accompanying drawing, be described in further detail.
Accompanying drawing explanation
Fig. 1 is operation panel structural representation;
Fig. 2 is bundle pectoral girdle partial structurtes schematic diagram;
Fig. 3 is anesthesia cup structure schematic diagram;
Fig. 4 is blood taking needle structural representation.
The specific embodiment
Operation panel 1 shown in Fig. 1 is rectangular flat board, dull and stereotyped middle part is provided with groove 2, the front end of groove is provided with draw-in groove 7, anesthesia tubule 8 is housed in draw-in groove, and the left side of groove is provided with bundle neck band 5, and the rear side of bundle neck band is provided with bundle pectoral girdle 3, the upper end of bundle neck band is provided with thread gluing 6 on bundle neck, the right side of groove is provided with thread gluing 9 under bundle neck, and the upper end of bundle pectoral girdle is provided with thread gluing 4 on bundle breast, and the right side of groove is provided with thread gluing 10 under bundle breast.
The bottom of the bundle pectoral girdle shown in Fig. 2 is fixed on operation panel, the upper middle part of bundle pectoral girdle is provided with an axis lubber-line 11, the right side of being close to lubber-line is provided with cardiac puncture hole 13, the right side of puncturing hole is provided with syringe needle fixed leg 14, the front end of axis lubber-line is provided with the forelimb line reference line 12 of experimental mouse, during blood sampling, blood taking needle needle handle is fixed on syringe needle fixed leg.
Anesthesia cup shown in Fig. 3 is comprised of cup 16 and base 15, in cup, be provided with anesthetic room 18, the bottom of anesthetic room is provided with ventilative separation net 19, in base, be provided with anesthetis apotheca 20, the upper end of base is provided with female thread, the downside of cup is provided with external screw thread, and cup is arranged on the upper end of base by being threaded, and the upper end of cup is provided with bowl cover 17.
The length of the blood taking needle syringe needle 21 shown in Fig. 4 is that after experimental mouse is fixed, the thickness of heart epithelium adds heart to the length of epidermis distance, and the needle handle of syringe needle upper end is provided with fixing hole 23, and the upper end of syringe needle is provided with connecting hose 22.
Mouse heart is close to axis between the 3rd rib and the 4th rib, yet the 3rd rib and the 4th rib are in subcutaneous and bad judgement, abecedarian often looks for inaccurate, even need to dissect the searching that a large amount of mouse practise heart, also there is cutting open thoracic cavity Puncture under direct vision heart, for heart, determine that the present invention adopts coordinate axes mode to determine, take white mice as example, after anesthesia, white mice forelimb journey naturally straight up, take forelimb line as transverse axis, the left of mice is to being positive direction, the axis of mice of take is the longitudinal axis, mouse tail is positive direction, unit is mm, measurement through a plurality of mices, the abscissa of its heart is [1, 3], vertical coordinate is [2, 8] getting its mean value coordinates is (2, 5).By above data, on carrying secretly, breast made axis compared with the corresponding mouse web portion of directrix axis, the corresponding two forelimb lines of two forelimb line reference lines, the corresponding heart mean value coordinates in cardiac puncture hole.The lowermost end of two forelimb upper arm parts that two described forelimb lines are mice and the connecting line of mice upper body.
The method step of heart puncturing extracting blood
1, first mice is put into anesthetic room and cover bottle cap, adopt inhalation anesthesia;
2, narcotic selection and the making of anaesthetizing cotton balls: anesthetis can be selected ether or chloroform, anesthetis is immersed in and on absorbent cotton, makes two anesthesia cotton balls of size, first large cotton balls is put into behind anesthetis locker room, again cup and base are screwed and be connected as a single entity, separately cotton pellet is put into anesthesia tubule, then anesthesia tubule is put into draw-in groove;
3, after mouse anesthesia, mice is lain on the back naturally in the groove of operation panel, adjust position and make its cervical region and bundle neck band, breast and bundle pectoral girdle horizontal alignment, first with bundle neck band, head is flattened, regulate anesthesia tubule to make mice nose be in contact with it and keep the state of inhalation anesthetic;
4, bundle pectoral girdle two ends are all that thread gluing connects, adjust bundle pectoral girdle, axis is alignd with mice axis compared with directrix, mouse web portion axis is comparatively obvious, bundle pectoral girdle is close to mice forelimb and is pressed straight down from upper, keep the corresponding mice two forelimb lines of forelimb reference line, considered the error between mice forelimb line and reference line in actual production, fixing rear puncturing hole is the puncture position of heart;
5, use disposable blood taking needle, hand-held needle handle firmly passes through puncturing hole fast, it punctures other histoorgan not worry syringe needle, because its needle length is special making, again by needle handle by fixing hole buckle on fixed leg syringe needle is fixing after, can slowly extract blood, be sure not the too fast anti-Hemostatic Oral Liquid of pull and have little time to be back to heart and cause dead mouse, blood drawing amount, depending on the size of done experimental mouse, extracts 30% of total blood volume and can not cause dead mouse.
The features such as the present invention is muroid heart puncturing extracting blood device, and experimenter, without can skillfully using through a large amount of exercises, saves a large amount of time, and this device has simply, quick, economic, safe, practical property is strong.
Claims (5)
1. the heart puncturing extracting blood device of medical research experiment muroid, comprise operation panel, anesthesia cup and blood taking needle, it is characterized in that: described operation panel (1) is rectangular flat board, dull and stereotyped middle part is provided with groove (2), the front end of groove is provided with draw-in groove (7), anesthesia tubule (8) is housed in draw-in groove, the left side of groove is provided with bundle neck band (5), the rear side of bundle neck band is provided with bundle pectoral girdle (3), the upper end of bundle neck band is provided with thread gluing (6) on bundle neck, the right side of groove is provided with thread gluing (9) under bundle neck, the upper end of bundle pectoral girdle is provided with thread gluing (4) on bundle breast, the right side of groove is provided with thread gluing (10) under bundle breast, the right side of operation panel is provided with anesthesia cup.
2. the heart puncturing extracting blood device of muroid is tested in medical research according to claim 1, it is characterized in that: the bottom of described bundle pectoral girdle is fixed on operation panel, the upper middle part of bundle pectoral girdle is provided with an axis lubber-line (11), the right side of being close to lubber-line is provided with cardiac puncture hole (13), the right side of puncturing hole is provided with syringe needle fixed leg (14), and the front end of axis lubber-line is provided with the forelimb line reference line (12) of experimental mouse.
3. the heart puncturing extracting blood device of muroid is tested in medical research according to claim 1, it is characterized in that: described anesthesia cup is comprised of cup (16) and base (15), in cup, be provided with anesthetic room (18), the bottom of anesthetic room is provided with ventilative separation net (19), in base, be provided with anesthetis apotheca (20), the upper end of base is provided with female thread, and the downside of cup is provided with external screw thread, cup is arranged on the upper end of base by being threaded, the upper end of cup is provided with bowl cover (17).
4. the heart puncturing extracting blood device of muroid is tested in medical research according to claim 2, it is characterized in that: the length of described blood taking needle syringe needle (21) is after experimental mouse is fixed, the thickness of heart epithelium adds heart to the length of epidermis distance, the needle handle of syringe needle upper end is provided with fixing hole (23), and the upper end of syringe needle is provided with connecting hose (22).
5. a heart puncturing extracting blood method for medical research experiment muroid, is characterized in that: it comprises the following steps:
(1) first mice is put into anesthetic room and cover bottle cap, adopt inhalation anesthesia;
(2) narcotic selection and the making of anaesthetizing cotton balls: anesthetis can be selected ether or chloroform, anesthetis is immersed in and on absorbent cotton, makes two anesthesia cotton balls of size, first large cotton balls is put into behind anesthetis locker room, again cup and base are screwed and be connected as a single entity, separately cotton pellet is put into anesthesia tubule, then anesthesia tubule is put into draw-in groove;
(3) after mouse anesthesia, mice is lain on the back naturally in the groove of operation panel, adjust position and make its cervical region and bundle neck band, breast and bundle pectoral girdle horizontal alignment, first with bundle neck band, head is flattened, regulate anesthesia tubule that mice nose is in contact with it and keep inhalation anesthetic state;
(4) bundle pectoral girdle two ends are all that thread gluing connects, adjust bundle pectoral girdle, axis is alignd with mice axis compared with directrix, mouse web portion axis is comparatively obvious, bundle pectoral girdle is close to mice forelimb and is pressed straight down from upper, keep the corresponding mice two forelimb lines of forelimb line reference line, fixing rear puncturing hole is the puncture position of heart;
(5) use disposable blood taking needle, hand-held needle handle firmly passes through puncturing hole fast, again by needle handle by fixing hole buckle on fixed leg syringe needle is fixing after, can slowly extract blood, being sure not the too fast anti-Hemostatic Oral Liquid of pull has little time to be back to heart and causes dead mouse, blood drawing amount, depending on the size of done experimental mouse, extracts 30% of total blood volume and can not cause dead mouse.
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| CN201410013716.5A CN103735277B (en) | 2014-01-13 | 2014-01-13 | Heart puncture blood collecting device for mice of medical research experiments |
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| CN201410013716.5A CN103735277B (en) | 2014-01-13 | 2014-01-13 | Heart puncture blood collecting device for mice of medical research experiments |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104688375A (en) * | 2015-03-11 | 2015-06-10 | 翁鉴科 | Laboratory mouse fixing device |
| CN106562835A (en) * | 2016-10-25 | 2017-04-19 | 苏州纽迈分析仪器股份有限公司 | Gaseous anesthesia carrying bed apparatus used for animal experiment |
| CN106725980A (en) * | 2016-12-30 | 2017-05-31 | 谷翠芝 | Rat Thoracicoabdominal puncture fixing device |
| CN107126221A (en) * | 2017-06-08 | 2017-09-05 | 隆林惠侬农业科技有限公司 | A kind of ox blood sampler |
| CN108420566A (en) * | 2018-03-09 | 2018-08-21 | 重庆医药高等专科学校 | Blood-taking device for experimental mouse |
| CN109009544A (en) * | 2018-07-26 | 2018-12-18 | 山东中医药大学 | A kind of Naoliqing capsule device and localization method |
| CN109124809A (en) * | 2018-09-19 | 2019-01-04 | 贵州医科大学附属医院 | A kind of Neonatal Mouse heart infarction model puncture outfit |
| CN111494716A (en) * | 2020-04-15 | 2020-08-07 | 青岛市妇女儿童医院（青岛市妇幼保健院、青岛市残疾儿童医疗康复中心、青岛市新生儿疾病筛查中心） | Heart surgery autologous pericardium patch processor and rapid anti-calcification treatment method |
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Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104688375A (en) * | 2015-03-11 | 2015-06-10 | 翁鉴科 | Laboratory mouse fixing device |
| CN106562835A (en) * | 2016-10-25 | 2017-04-19 | 苏州纽迈分析仪器股份有限公司 | Gaseous anesthesia carrying bed apparatus used for animal experiment |
| CN106562835B (en) * | 2016-10-25 | 2018-01-19 | 苏州纽迈分析仪器股份有限公司 | A kind of gas anesthesia for zoopery carries bed apparatus |
| CN106725980A (en) * | 2016-12-30 | 2017-05-31 | 谷翠芝 | Rat Thoracicoabdominal puncture fixing device |
| CN107126221A (en) * | 2017-06-08 | 2017-09-05 | 隆林惠侬农业科技有限公司 | A kind of ox blood sampler |
| CN108420566A (en) * | 2018-03-09 | 2018-08-21 | 重庆医药高等专科学校 | Blood-taking device for experimental mouse |
| CN109009544A (en) * | 2018-07-26 | 2018-12-18 | 山东中医药大学 | A kind of Naoliqing capsule device and localization method |
| CN109009544B (en) * | 2018-07-26 | 2021-02-23 | 山东中医药大学 | Brain stereotaxic apparatus |
| CN109124809A (en) * | 2018-09-19 | 2019-01-04 | 贵州医科大学附属医院 | A kind of Neonatal Mouse heart infarction model puncture outfit |
| CN109124809B (en) * | 2018-09-19 | 2023-09-01 | 贵州医科大学附属医院 | Neonatal suckling mouse myocardial infarction model puncture outfit |
| CN111494716A (en) * | 2020-04-15 | 2020-08-07 | 青岛市妇女儿童医院（青岛市妇幼保健院、青岛市残疾儿童医疗康复中心、青岛市新生儿疾病筛查中心） | Heart surgery autologous pericardium patch processor and rapid anti-calcification treatment method |
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| CN103735277B (en) | 2015-05-13 |
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