CN109122254A - A kind of haloduric corn germplasm quickly, efficiently, identification method can be transplanted - Google Patents
A kind of haloduric corn germplasm quickly, efficiently, identification method can be transplanted Download PDFInfo
- Publication number
- CN109122254A CN109122254A CN201810794952.3A CN201810794952A CN109122254A CN 109122254 A CN109122254 A CN 109122254A CN 201810794952 A CN201810794952 A CN 201810794952A CN 109122254 A CN109122254 A CN 109122254A
- Authority
- CN
- China
- Prior art keywords
- salt
- corn
- germplasm
- transplanting
- efficiently
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/02—Germinating apparatus; Determining germination capacity of seeds or the like
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Health & Medical Sciences (AREA)
- Physiology (AREA)
- Soil Sciences (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The present invention disclose haloduric corn germplasm quickly, efficiently, identification method can be transplanted, including Seed of maize inbred is sprouted；The seed of sprouting is placed on transplanting disk and is stored；Multiple transplanting disks are respectively put into different vessels, and the bottom interior wall of the bottom and container of transplanting disk is equipped with predetermined gap in height, and corn mill water culture nutrient solution is filled in container；Add salt treatment in partial containers nutrient solution, salt is not added in other corresponding container nutrient solutions as control；After corn cotyledon is fully deployed, remove corn embryosperm；Deciding grade and level evaluation and screening germplasm materials, it screens germplasm materials and uses following processing mode: first is that obtaining mutant material by screening, transplanting is carried out when occurring salt tolerant single plant in salt density value material to be ensured normally to pollinate solid, carries out the genetic researches such as favorable genes excavating resource；Second is that carrying out whole transplanting to Salt-tolerant Materials are stablized, salt tolerant experimental study or breeding research are carried out.The present invention is simple, quick, efficient, lossless transplants haloduric corn Germplasm Identification.
Description
Technical field
The present invention relates to corn Salt tolerance and identification method technical field, in particular to a kind of haloduric corn germplasm is fast
Speed, efficiently, identification method can be transplanted.
Background technique
The salinization of soil is the main restrictive factor of coastal region, semiarid zone agricultural production, salt-soda soil main feature
It is to be easy to appear salting stain, it is irregular uneven.Corn is China the first generalized grain crop, is the nonirrigated farmland transition silos of wide adaptability
Crop, yield potentiality are high.Currently, salt tolerant high-yield corn kind extremely lacks, and key constraints are Maize Germplasm Base scarcities,
Especially haloduric corn germplasm materials more lack, few by the internal salt-tolerant mutant material that screening obtains that makes a variation, and lack system
The Mechanism Study of system.Currently, the means for studying corn salt/alkali-tolerance are much limited, and field test is not easy to control, and identification is accurate
Property is not high, and it is not high that saline-alkali tolerant breeding by germplasm materials scarcity is influenced effect；In addition, containing a large amount of nutrients in corn kernel
Matter screens Salt-Tolerance Identification to Maize at Seedling Stage water planting mode and is affected.Therefore by preparing accurate, uniform auxotype salt
Water carries out the operation of endosperm in seedling stage, specifies Rapid identification evaluation method, to solve rapidly and efficiently salt-alkali tolerant corn germplasm
The problem of identification, while by the method for lossless transplanting, solving the Salinity tolerance germplasm filtered out or mutant material restoration ecosystem just
The problem of often utilizing, provides feasible method for saline-alkali tolerant genetic breeding and molecular biology research.
Summary of the invention
It is fast the object of the invention is in order to solve the above problems existing in the present technology, provide a kind of haloduric corn germplasm
Speed, efficiently, the mutant material that can transplant identification method, and obtain to screening directly carries out favorable genes excavating resource and utilizes to grind
Study carefully, genetic research or breeding research are carried out to the salt-tolerant inbred lines that screening obtains；The present invention can be simple, fast and efficiently first
Characterization and evaluation corn germplasm salt tolerance is walked, and carries out not damaged transplanting and is used；It is in addition of the invention by way of removing endosperm,
Influence identify selection of salt tolerance of seed nutritional substance is reduced, the efficiency and accurately of salt tolerance of corn screening and identification is greatly improved
Property, while needing removable and cultivation to restore normal growth according to research, overcome the disadvantage of field test inaccuracy.This method is adopted
Instrument and equipment and medicine and reagent is at low cost and easy buying, the experimental method in the present invention was simple to operation, at 1 month
Time in, 1 technical staff can complete the Rapid identification of hundreds of parts of corn germplasm materials.
The technical scheme adopted by the invention is as follows:
A kind of haloduric corn germplasm quickly, efficiently, identification method can be transplanted, include the next steps:
1) Seed of maize inbred is sprouted；
2) seed of sprouting is placed on multiple transplanting disks and is stored；
3) multiple transplanting disks are respectively put into different containers, the bottom interior wall of the bottom and container of transplanting disk is in height
Equipped with predetermined gap, corn mill water culture nutrient solution is filled in container；
4) add salt treatment in partial containers nutrient solution, salt conduct pair is not added in other corresponding container nutrient solutions
According to；
5) after corn cotyledon is fully deployed, remove corn embryosperm；
6) deciding grade and level evaluation and screening germplasm materials, screening germplasm materials use following processing mode: first is that by screening acquisition
Mutant material, when occurring salt tolerant single plant in salt density value material, carry out transplanting ensure normally to pollinate it is solid, carry out heredity grind
Study carefully；Second is that carrying out whole transplanting breeding research, the continuation various places such as salt tolerant experimental study or breeding research to Salt-tolerant Materials are stablized
Reason.
The step 1) sprouts Seed of maize inbred using sand culture method, and Germination Temperature is 28 DEG C, sprouts bud
A length of 1.5cm.
Transplanting disk in the step 2) is equipped with multiple sprouting bud placed holes, is connected with netted branch in the bottom end of transplanting disk
Fagging, the bottom end of mesh-supported plate and the bottom interior wall of container are equipped with scheduled gap, and the bottom root for sprouting bud is passed down through
Mesh.
The transplanting disk is made of the foamed plastics of 6-12cm thickness, mesh-supported plate using thin plastic net-board or
Metal net at.
Step 3) the corn mill water culture nutrient solution is Hoagland nutrient solution.
Add when salt period being added a piece of expansion leaf occur for the seed after corn germinal of salt treatment in the step 4).
The step 5) removes corn embryosperm using tweezers.
The deciding grade and level identification of the step 6) uses following procedure: it is determined with control container corn growing way and starts qualification time, 2
1 heart stage of leaf is the Initial Characterization time, often grows 1 expansion leaf and carries out identification 1 time, and identification 5 times to 1 heart stage of 6 leaf altogether；
Evaluation criterion is as follows: with maize seedling leaf withering degree and the withered degree of whole strain, maize seedling leaf withering degree and whole strain
The time of withered degree is divided into 1,3,5,7,9 totally 5 ranks, is corresponding in turn to as germplasm materials brief appraisal standard
For salt tolerant, moderate salt tolerant, medium growing way, moderate salt density value, salt density value.
Beneficial effects of the present invention:
1. present invention deciding grade and level evaluation program and evaluation criterion is simple, strong operability, with maize seedling leaf withering degree and whole
The withered degree of strain, maize seedling leaf withering degree and whole strain wither degree time as germplasm materials brief appraisal standard,
Can it is simple, fast and efficiently Preliminary Identification evaluates corn germplasm salt tolerance, and carries out free of losses transplanting and is used, test
Method is simple to operation, and within 1 month time, 1 technical staff can complete the quick mirror of hundreds of parts of corn germplasm materials
It is fixed, it can must greatly improve test and determination rates compared with prior art, save appraisal cost.
2. obtaining mutant material by screening, when occurring salt tolerant single plant in salt density value material, carrying out transplanting ensures just
It often pollinates solid, carries out the genetic researches such as favorable genes excavating resource；It can also be educated to the whole transplanting of Salt-tolerant Materials progress is stablized
Kind research continues the various processing such as salt tolerant experimental study or breeding research.；This method can to the Saline alkali tolerance evaluation of corn germplasm
Using as germplasm saline-alkali tolerant characteristic, and carry out the important evidence of saline-alkali tolerant breeding utilization and genetic research, it is resistance to after being identified
Salt germplasm, sensitive germplasm or salt related mutants seedling not damaged can transplant, and carry out further research and utilization, and seedling growth
With untreated material indifference.This method can be used for the screening of salt-alkali tolerant corn mutant material, screening corn salt/alkali-tolerance germplasm
With saline-alkali tolerant breeding.
3. the present invention by way of removing endosperm, reduces the influence that seed nutritional substance identifies selection of salt tolerance, greatly
The efficiency and accuracy of salt tolerance of corn screening and identification is improved, while needing removable and cultivation to restore normal growth according to research, gram
The disadvantage of field test inaccuracy is taken.
4. instrument and equipment and medicine and reagent that the present invention uses, including foamed plastics transplant disk, mesh-supported plate, tweezer
The buyings at low cost and easy such as son, Hoagland nutrient solution.
Detailed description of the invention
The present invention is described further with reference to the accompanying drawings and examples:
Fig. 1 is the structural schematic diagram of transplanting disk of the invention together with container combination；
Fig. 2 is the cross-sectional view of Fig. 1.
Specific embodiment
Technical solution in order to enable those skilled in the art to better understand the present invention, below in conjunction with of the invention real
The attached drawing in example is applied, technical scheme in the embodiment of the invention is clearly and completely described, it is clear that described implementation
Example is only a part of the embodiment of the present invention, instead of all the embodiments.
As shown in Figs. 1-2, a kind of haloduric corn germplasm quickly, efficiently, identification method can be transplanted, include the next steps:
1) 1) Seed of maize inbred is sprouted；
2) 2) seed of sprouting 5 is placed on multiple transplanting disks 2 on and is stored；
3) multiple transplanting disks are respectively put into different containers 1, transplant the bottom of disk and the bottom interior wall of container in height
It is equipped with predetermined gap, corn mill water culture nutrient solution 6 is filled in container；
4) add salt treatment in partial containers nutrient solution, salt conduct pair is not added in corresponding other container nutrient solutions
According to；
5) after corn cotyledon is fully deployed, remove corn embryosperm；
6) deciding grade and level evaluation and screening germplasm materials, screening germplasm materials use following processing mode: first is that by screening acquisition
Mutant material, when occurring salt tolerant single plant in salt density value material, carry out transplanting ensure normally to pollinate it is solid, carry out heredity grind
Study carefully；Second is that carrying out whole transplanting breeding research, the continuation various places such as salt tolerant experimental study or breeding research to Salt-tolerant Materials are stablized
Reason.
The step 1) sprouts Seed of maize inbred using sand culture method, and Germination Temperature is 28 DEG C, sprouts bud
A length of 1.5cm.
Transplanting disk in the step 2) is equipped with multiple sprouting bud placed holes 4, is connected in the bottom end of transplanting disk netted
Support plate 3, the bottom end of mesh-supported plate 3 and the bottom interior wall of container are equipped with scheduled gap, and the bottom root for sprouting bud is downward
Across mesh.
The transplanting disk is made of the foamed plastics of 6-12cm thickness, mesh-supported plate using thin plastic net-board or
Metal net at.
Step 3) the corn mill water culture nutrient solution is Hoagland nutrient solution.
Add when salt period being added a piece of expansion leaf occur for the seed after corn germinal of salt treatment in the step 4).
The step 5) removes corn embryosperm using tweezers.
The deciding grade and level identification of the step 6) uses following procedure: it is determined with control container corn growing way and starts qualification time, 2
1 heart stage of leaf is the Initial Characterization time, often grows 1 expansion leaf and carries out identification 1 time, and identification 5 times to 1 heart stage of 6 leaf altogether；
Evaluation criterion is as follows: with maize seedling leaf withering degree and the withered degree of whole strain, maize seedling leaf withering degree and whole strain
The time of withered degree is divided into 1,3,5,7,9 totally 5 ranks, is corresponding in turn to as germplasm materials brief appraisal standard
For salt tolerant, moderate salt tolerant, medium growing way, moderate salt density value, salt density value.
Based on the embodiments of the present invention, those of ordinary skill in the art are obtained without making creative work
The every other embodiment obtained, should fall within the scope of the present invention.
Claims (8)
1. a kind of haloduric corn germplasm quickly, efficiently, identification method can be transplanted, characterized in that the following steps are included:
1) Seed of maize inbred is sprouted；
2) seed of sprouting is placed on multiple transplanting disks and is stored；
3) multiple transplanting disks are respectively put into different containers, the bottom interior wall of the bottom and container of transplanting disk is equipped in height
Predetermined gap fills corn mill water culture nutrient solution in container；
4) add salt treatment in partial containers nutrient solution, salt is not added in other corresponding container nutrient solutions as control；
5) after corn cotyledon is fully deployed, remove corn embryosperm；
6) deciding grade and level evaluation and screening germplasm materials, screening germplasm materials use following processing mode: first is that being mutated by screening
Body material, when occurring salt tolerant single plant in salt density value material, carry out transplanting ensure normally to pollinate it is solid, carry out genetic research；Two
It is that Salt-tolerant Materials progress is whole to transplant breeding research, continuation salt tolerant experimental study or the various processing of breeding research to stablizing.
2. a kind of haloduric corn germplasm as described in claim 1 quickly, efficiently, identification method can be transplanted, characterized in that it is described
Step 1) sprouts Seed of maize inbred using sand culture method, standard: Germination Temperature is 28 DEG C, and it is a length of to sprout bud
1.5cm(Figure 1A)。
3. a kind of haloduric corn germplasm as described in claim 1 quickly, efficiently, identification method can be transplanted, characterized in that it is described
Transplanting disk in step 2) is equipped with multiple sprouting bud placed holes, is connected with mesh-supported plate, netted branch in the bottom end of transplanting disk
The bottom end of fagging and the bottom interior wall of container are equipped with scheduled gap, and the bottom root for sprouting bud is passed down through mesh.
4. a kind of haloduric corn germplasm as claimed in claim 3 quickly, efficiently, identification method can be transplanted, characterized in that it is described
Transplanting disk be made of the foamed plastics of 6-12cm thickness, mesh-supported plate using thin plastic net-board or metal net at.
5. a kind of haloduric corn germplasm as described in claim 1 quickly, efficiently, identification method can be transplanted, characterized in that it is described
Step 3) corn mill water culture nutrient solution is Hoagland nutrient solution.
6. a kind of haloduric corn germplasm as described in claim 1 quickly, efficiently, identification method can be transplanted, characterized in that it is described
Add when salt period being added a piece of expansion leaf occur for the seed after corn germinal of salt treatment in step 4).
7. a kind of haloduric corn germplasm as described in claim 1 quickly, efficiently, identification method can be transplanted, characterized in that it is described
Step 5) removes corn embryosperm using tweezers.
8. a kind of haloduric corn germplasm as described in claim 1 quickly, efficiently, identification method can be transplanted, characterized in that it is described
The deciding grade and level identification of step 6) uses following procedure: being determined with control container corn growing way and starts qualification time, 1 heart stage of 2 leaf is first
Beginning qualification time, often grows 1 expansion leaf and carries out identification 1 time, identify altogether 5 times to 1 heart stage of 6 leaf；
Evaluation criterion is as follows: withered with maize seedling leaf withering degree and the withered degree of whole strain, maize seedling leaf withering degree and whole strain
The time of degree is divided into 1,3,5,7,9 totally 5 ranks, it is resistance to for being corresponding in turn to as germplasm materials brief appraisal standard
Salt, moderate salt tolerant, medium growing way, moderate salt density value, salt density value.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810794952.3A CN109122254A (en) | 2018-07-19 | 2018-07-19 | A kind of haloduric corn germplasm quickly, efficiently, identification method can be transplanted |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810794952.3A CN109122254A (en) | 2018-07-19 | 2018-07-19 | A kind of haloduric corn germplasm quickly, efficiently, identification method can be transplanted |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109122254A true CN109122254A (en) | 2019-01-04 |
Family
ID=64800937
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810794952.3A Pending CN109122254A (en) | 2018-07-19 | 2018-07-19 | A kind of haloduric corn germplasm quickly, efficiently, identification method can be transplanted |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109122254A (en) |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1953655A (en) * | 2004-04-09 | 2007-04-25 | 夏威夷大学 | Floating plant cultivation platform and method for growing terrestrial plants in saline water of various salinities for multiple purposes |
| CN102524054A (en) * | 2011-12-20 | 2012-07-04 | 东北农业大学 | Method for screening salt-tolerant genetically modified corn |
| CN104012267A (en) * | 2014-04-30 | 2014-09-03 | 中国农业科学院作物科学研究所 | Maize salt tolerance identification method |
| CN106416773A (en) * | 2016-10-09 | 2017-02-22 | 山东省农业科学院玉米研究所 | Screening and identification method of salt tolerance of corns at seedling stage |
-
2018
- 2018-07-19 CN CN201810794952.3A patent/CN109122254A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1953655A (en) * | 2004-04-09 | 2007-04-25 | 夏威夷大学 | Floating plant cultivation platform and method for growing terrestrial plants in saline water of various salinities for multiple purposes |
| CN102524054A (en) * | 2011-12-20 | 2012-07-04 | 东北农业大学 | Method for screening salt-tolerant genetically modified corn |
| CN104012267A (en) * | 2014-04-30 | 2014-09-03 | 中国农业科学院作物科学研究所 | Maize salt tolerance identification method |
| CN106416773A (en) * | 2016-10-09 | 2017-02-22 | 山东省农业科学院玉米研究所 | Screening and identification method of salt tolerance of corns at seedling stage |
Non-Patent Citations (3)
| Title |
|---|
| 何启伟 等: "《山东蔬菜科技进步与产业发展》", 31 December 2006, 中国农业出版社 * |
| 吴丹丹: "玉米苗期耐盐性的QTL定位", 《中国优秀硕士学位论文全文数据库 农业科技辑》 * |
| 翁跃进: "《作物耐盐品种及其栽培技术》", 31 January 2003, 中国农业出版社 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Turner | Imposing and maintaining soil water deficits in drought studies in pots | |
| Mavi et al. | Mean germination time estimates the relative emergence of seed lots of three cucurbit crops under stress conditions | |
| CN103828713A (en) | Saline-alkaline tolerant germplasm screening and high-yield cultivating method of barley | |
| CN103238449B (en) | Method for rapidly identifying and selecting salt-tolerant peanut varieties in room | |
| CN102487771A (en) | Heading stage high-temperature resistance identifying method for rice variety | |
| CN102577798A (en) | Method for identifying salt tolerance of corn | |
| Kaur et al. | Screening of mungbean genotypes for drought tolerance using different water potential levels | |
| Aspinall et al. | An analysis of competition between barley and white persicaria: I. The effects on growth | |
| Sharif-Zadeh et al. | The effects of different maturation conditions on seed dormancy and germination of Cenchrus ciliaris | |
| CN108966729A (en) | A kind of characterization and evaluation method of Maize at Seedling Stage salt tolerance | |
| CN106069675A (en) | A kind of leaf vegetables plant soil-less culturing substrate and cultural method thereof | |
| CN104255404A (en) | Method for rapid hydroponic identification of cotton germplasm salt tolerance and application | |
| CN105359856A (en) | Method and device for batch identification of wheat salt tolerance | |
| JAYA et al. | Responses of leaf celery to floating culture system with different depths of water-substrate interface and NPK-fertilizer application | |
| CN116671395A (en) | Cold resistance identification method for forage germplasm resources | |
| Souza et al. | Patents for the physiological quality in seeds of peach rootstock classified by weight and stored for different periods | |
| CN109122254A (en) | A kind of haloduric corn germplasm quickly, efficiently, identification method can be transplanted | |
| CN108450272B (en) | Method for identifying salt tolerance of cotton in seedling stage | |
| CN107432222A (en) | A kind of authentication method of the cold-resistant phenotype of rice seedling | |
| CN109287420B (en) | Method for rapidly predicting oil content of peanuts | |
| CN105660335A (en) | Method for identifying moisture-resistant barley variety and system | |
| Walenta et al. | Vernalization response of plants grown from spikelets of spring and fall cohorts of jointed goatgrass | |
| Ghassemi-Golezani et al. | Development of mung-bean seed vigour under different irrigations and plant densities | |
| CN108077002A (en) | A kind of rice takes root the new identification method of ability | |
| Karpova et al. | The efficiency of application of complex liquid fertilizers in chelated form against the background of natural and mineral nutrition of spring wheat plants |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20190104 |
|
| RJ01 | Rejection of invention patent application after publication |