CN109109316B - Biological 3D printing apparatus with clean temperature control function that disinfects - Google Patents
Biological 3D printing apparatus with clean temperature control function that disinfects Download PDFInfo
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- CN109109316B CN109109316B CN201810917763.0A CN201810917763A CN109109316B CN 109109316 B CN109109316 B CN 109109316B CN 201810917763 A CN201810917763 A CN 201810917763A CN 109109316 B CN109109316 B CN 109109316B
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B29—WORKING OF PLASTICS; WORKING OF SUBSTANCES IN A PLASTIC STATE IN GENERAL
- B29C—SHAPING OR JOINING OF PLASTICS; SHAPING OF MATERIAL IN A PLASTIC STATE, NOT OTHERWISE PROVIDED FOR; AFTER-TREATMENT OF THE SHAPED PRODUCTS, e.g. REPAIRING
- B29C64/00—Additive manufacturing, i.e. manufacturing of three-dimensional [3D] objects by additive deposition, additive agglomeration or additive layering, e.g. by 3D printing, stereolithography or selective laser sintering
- B29C64/20—Apparatus for additive manufacturing; Details thereof or accessories therefor
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B29—WORKING OF PLASTICS; WORKING OF SUBSTANCES IN A PLASTIC STATE IN GENERAL
- B29C—SHAPING OR JOINING OF PLASTICS; SHAPING OF MATERIAL IN A PLASTIC STATE, NOT OTHERWISE PROVIDED FOR; AFTER-TREATMENT OF THE SHAPED PRODUCTS, e.g. REPAIRING
- B29C64/00—Additive manufacturing, i.e. manufacturing of three-dimensional [3D] objects by additive deposition, additive agglomeration or additive layering, e.g. by 3D printing, stereolithography or selective laser sintering
- B29C64/30—Auxiliary operations or equipment
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B29—WORKING OF PLASTICS; WORKING OF SUBSTANCES IN A PLASTIC STATE IN GENERAL
- B29C—SHAPING OR JOINING OF PLASTICS; SHAPING OF MATERIAL IN A PLASTIC STATE, NOT OTHERWISE PROVIDED FOR; AFTER-TREATMENT OF THE SHAPED PRODUCTS, e.g. REPAIRING
- B29C64/00—Additive manufacturing, i.e. manufacturing of three-dimensional [3D] objects by additive deposition, additive agglomeration or additive layering, e.g. by 3D printing, stereolithography or selective laser sintering
- B29C64/30—Auxiliary operations or equipment
- B29C64/386—Data acquisition or data processing for additive manufacturing
- B29C64/393—Data acquisition or data processing for additive manufacturing for controlling or regulating additive manufacturing processes
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B33—ADDITIVE MANUFACTURING TECHNOLOGY
- B33Y—ADDITIVE MANUFACTURING, i.e. MANUFACTURING OF THREE-DIMENSIONAL [3-D] OBJECTS BY ADDITIVE DEPOSITION, ADDITIVE AGGLOMERATION OR ADDITIVE LAYERING, e.g. BY 3-D PRINTING, STEREOLITHOGRAPHY OR SELECTIVE LASER SINTERING
- B33Y30/00—Apparatus for additive manufacturing; Details thereof or accessories therefor
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B33—ADDITIVE MANUFACTURING TECHNOLOGY
- B33Y—ADDITIVE MANUFACTURING, i.e. MANUFACTURING OF THREE-DIMENSIONAL [3-D] OBJECTS BY ADDITIVE DEPOSITION, ADDITIVE AGGLOMERATION OR ADDITIVE LAYERING, e.g. BY 3-D PRINTING, STEREOLITHOGRAPHY OR SELECTIVE LASER SINTERING
- B33Y40/00—Auxiliary operations or equipment, e.g. for material handling
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
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- Manufacturing & Machinery (AREA)
- Optics & Photonics (AREA)
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
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- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
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- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Molecular Biology (AREA)
- Apparatus For Disinfection Or Sterilisation (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses biological 3D printing equipment with a clean sterilization temperature control function, and belongs to the technical field of biological 3D printing. The biological 3D printing apparatus includes: the heat insulation box is arranged on the base, and an air inlet and an air outlet are formed in the heat insulation box; the printing structure is arranged in the heat insulation box and used for printing the three-dimensional model on the printing platform; the air outlet of the air filtering structure is communicated with the air inlet of the heat preservation box through an air duct, the air inlet is communicated with the air outlet of the heat preservation box, and the air filtering structure is used for sucking air in the heat preservation box for filtering; the temperature control structure is arranged between the air outlet of the air filtering structure and the air duct and is used for cooling the filtered air; the disinfection structure is arranged at the top of the heat preservation box and used for disinfecting and sterilizing the air after being cooled, and the air enters the heat preservation box from the air inlet through the air channel. The biological 3D printing equipment provided by the invention gets rid of dependence on a biological safety cabinet, is more convenient to use and has a wide application range.
Description
Technical Field
The invention belongs to the technical field of biological manufacturing and biological 3D printing, and particularly relates to biological 3D printing equipment integrating cleaning, sterilization and temperature control.
Background
The existing biological 3D printing technology combines 3D printing technology with medical, biological materials and computer technology to create 3D models for patient specific anatomical structures and physiology. The biological 3D printer is a device which can position and assemble biological materials or cell units according to the additive manufacturing principle under the drive of a digital three-dimensional model, manufacture products such as medical instruments, tissue engineering scaffolds, tissue organs and the like, disperse the model into a plurality of layers by reading in the three-dimensional model reconstructed or designed by medical image data, and control a printing nozzle to print the biological materials or cells layer by a computer until the printing is finished.
Due to the particularity of biological 3D printing, a clean and sterile printing environment and control of different printing temperatures are required, however, the current biological 3D printer needs to ensure the clean and sterile printing environment by means of a biological safety cabinet or other conditions; because the variety of the biological materials is more, the required temperature is different, and for the temperature control system of the existing biological printer, the biological materials are easy to block, the operation is complicated, and the use is limited.
The 3D printer applied to the field of biological printing has higher requirements on the printing environment during working, and the temperature control on the environment of the forming chamber of the printer is important on the one hand except the control on the cleanliness and the humidity of the environment. Because most of the materials applied to the field of biological 3D printing are temperature-sensitive materials or isolated cells with requirements on specific temperature, a forming chamber is required to be adjusted to reach a specific temperature point or temperature range during printing operation, and the temperature is required to be subjected to gradient control sometimes.
Most biological 3D printers at present often only carry out temperature control to the shower nozzle and the shaping platform of printer, and at the actual printing in-process, there is the control by temperature change blind area in material or isolated cell behind the shower nozzle and before the contact shaping platform, and this is unfavorable for the survival rate and the printing accuracy control of cell. Although the required ambient temperature can be obtained by placing the entire printer in a room where temperature control is possible, the control accuracy of this method is low and unstable, and the requirement for bioprinting cannot be satisfied.
Disclosure of Invention
The invention aims to solve the problems that the existing biological 3D printer needs to be placed in a room capable of controlling temperature to obtain the required environment temperature, the temperature control precision of the method is low and unstable, and the requirement of biological printing cannot be met.
The invention discloses biological 3D printing equipment with a clean sterilization temperature control function, which comprises:
the heat insulation box is arranged on the base, and an air inlet and an air outlet are formed in the heat insulation box;
the printing structure is arranged in the heat insulation box and used for printing the three-dimensional model on the printing platform;
the air outlet of the air filtering structure is communicated with the air inlet of the heat insulation box through an air duct, the air inlet of the air filtering structure is communicated with the air outlet of the heat insulation box, and the air filtering structure is used for sucking air in the heat insulation box for filtering;
the temperature control structure is arranged between the air outlet of the air filtering structure and the air channel and is used for cooling the filtered air;
the sterilization structure is arranged at the top of the incubator and used for sterilizing the cooled air, and the air enters the incubator from the air inlet through the air channel.
Preferably, the printing structure is arranged on the base, and the heat insulation box is positioned at the upper part of the base;
the printing structure includes:
the Y-axis moving part is horizontally arranged on the base, the printing platform is arranged on the Y-axis moving part, and the Y-axis moving part is used for driving the printing platform to move along the Y direction;
the gantry is vertically fixed on the upper part of the base and is positioned on one side of the base;
the X-axis movement part is arranged on the gantry, and the movement direction of the X-axis movement part is vertical to that of the Y-axis movement part;
the Z-axis moving part is arranged on the X-axis moving part, and the moving direction of the Z-axis moving part is vertical to the moving direction of the Y-axis moving part and the moving direction of the X-axis moving part;
the E-axis moving part is arranged on the Z-axis moving part;
and the spray head part is arranged on the Z-axis moving part, a spray head of the spray head part faces the printing platform, and the E-axis moving part is used for controlling the pushing or pulling of a piston in the spray head part.
Preferably, the air filtering structure adopts an FFU fan filter unit.
Preferably, the temperature control structure comprises a semiconductor refrigeration piece and/or an air compressor.
Preferably, the sterilization structure adopts an ultraviolet sterilization lamp, and the ultraviolet sterilization lamp is used for sterilizing bacteria and viruses in the air.
Preferably, the heat preservation box further comprises a shell, and the air filtering structure, the temperature control structure and the disinfection and sterilization structure are all located inside the shell.
Preferably, air inlets are formed in two sides of the interior of the heat insulation box.
Preferably, a fan is arranged at the air inlet to suck air from the air duct.
The features mentioned above can be combined in various suitable ways or replaced by equivalent features as long as the object of the invention is achieved.
The biological 3D printing equipment with the clean sterilization and temperature control functions breaks away from dependence on a biological safety cabinet, provides a clean and sterile environment with controllable temperature for biological 3D printing through the air filtering structure, the temperature control structure and the disinfection and sterilization structure so as to meet the printing requirements of high-temperature materials and low-temperature materials, can position and assemble biological materials or living cells, and can manufacture biomedical products such as artificial implantation stents, tissue organs, medical auxiliary tools and the like, so that the biological 3D printing equipment is more convenient to use and has a wide application range.
Drawings
FIG. 1 is a schematic perspective view of a biological 3D printing apparatus with a sterilization and temperature control function according to an embodiment of the present invention;
fig. 2 is a cross-sectional view a-a of fig. 1.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
It should be noted that the embodiments and features of the embodiments may be combined with each other without conflict.
The invention is further described with reference to the following drawings and specific examples, which are not intended to be limiting.
As shown in fig. 1-2, a biological 3D printing apparatus with a clean sterilization and temperature control function includes:
the heat preservation box 3 is arranged on the base 1, and an air inlet and an air outlet are formed in the heat preservation box 3;
the printing structure is arranged in the heat insulation box 3 and used for printing the three-dimensional model on the printing platform 2;
the air outlet of the air filtering structure is communicated with the air inlet of the heat preservation box 3 through an air duct, the air inlet is communicated with the air outlet of the heat preservation box 3, and the air filtering structure is used for sucking air in the heat preservation box 3 for filtering;
the temperature control structure is arranged between the air outlet of the air filtering structure and the air channel and is used for cooling the filtered air;
the disinfection structure is arranged at the top of the heat preservation box and used for disinfecting and sterilizing the air after being cooled, and the air enters the heat preservation box 3 from the air inlet through the air channel.
Further, the temperature control structure may include a semiconductor chilling plate 4, and/or an air compressor.
In this embodiment, biological 3D printing apparatus with clean temperature control function that disinfects has broken away from the reliance to the biosafety cabinet, through the filtration structure, temperature control structure and disinfection structure of disinfecting, realize providing the controllable clean sterile environment of temperature for biological 3D prints, with the printing requirement of satisfying high temperature material and low temperature material, can fix a position assembly biomaterial or living cell, make the artifical biomedical products such as implanting support, tissue organ and medical appurtenance, it is more convenient to use, wide application range.
As shown in fig. 1-2, in a preferred embodiment,
the printing structure is arranged on the base 1, and the heat insulation box 3 is positioned on the upper part of the base 1;
the printing structure may include:
the Y-axis moving part 11 is horizontally arranged on the base 1, the printing platform 2 is arranged on the Y-axis moving part 11, and the Y-axis moving part 11 is used for driving the printing platform 2 to move along the Y direction;
the gantry 8 is vertically fixed at the upper part of the base 1 and is positioned at one side of the base 1;
the X-axis movement part 6 is arranged on the gantry 8, and the movement direction of the X-axis movement part 6 is vertical to the movement direction of the Y-axis movement part 11;
a Z-axis moving part 7 arranged on the X-axis moving part 6, wherein the moving direction of the Z-axis moving part 7 is vertical to the moving direction of the Y-axis moving part 11 and the moving direction of the X-axis moving part 6;
an E-axis moving part 9 arranged on the Z-axis moving part 7;
the nozzle part 10 is arranged on the Z-axis moving part 7, the nozzle of the nozzle part 10 faces the printing platform 2, and the E-axis moving part 9 is used for controlling the pushing or pulling of the piston in the nozzle part 10.
In the present embodiment, the Y-axis moving unit 11 is mounted on the base 1, the printing table 2 is mounted on the Y-axis moving unit 11, the X-axis moving unit 6 is mounted on the gantry 8, the Z-axis moving unit 7 is mounted on the X-axis moving unit 6, the E-axis moving unit 9 is mounted on the Z-axis moving unit 7, and the head unit 10 is mounted on the Z-axis moving unit 7. The Y-axis movement part 11, the X-axis movement part 6 and the Z-axis movement part 7 finish driving the spray head part 10 to move along the Y-axis direction, the X-axis direction and the Z-axis direction. The head section 10 is directed downward toward the printing platform 2, and the advance or the withdrawal of the piston in the head section 10 is controlled by the E-axis moving section 9.
In a preferred embodiment, the air filtration structure may employ an FFU fan filter unit 5.
In this embodiment, the FFU fan filter unit 5 is provided with a primary and a high-efficiency two-stage filter screen, which can improve the air filtration efficiency, and the FFU fan filter unit 5 adopts an outer rotor centrifugal fan, which has the characteristics of long service life, low noise, maintenance-free, small vibration, stepless speed regulation and the like. The method is suitable for obtaining higher-level clean environment in various environments.
In a preferred embodiment, the sterilization structure may employ an ultraviolet sterilization lamp 12, and the ultraviolet sterilization lamp 12 is used to sterilize bacteria and viruses in the air.
In the present embodiment, the ultraviolet disinfection lamp 12 can be used to kill bacteria propagules, spores, mycobacteria, coronaviruses, fungi, rickettsia, chlamydia, etc., so as to provide a sterile environment for biological 3D printing, and the printing platform 2 and the biological materials printed on the printing platform 2 can be printed in the sterile environment.
In a preferred embodiment, the incubator 3 may further comprise an outer casing, and the air filtering structure, the temperature control structure and the sterilization structure are all located inside the outer casing.
In this embodiment, the thermal insulation box 3 plays a thermal insulation role for the printing platform 2 and the biological material printed on the printing platform 2.
In a preferred embodiment, air inlets are formed on both sides of the interior of the thermal insulation box 3, which helps to circulate air in the thermal insulation box 3 and makes the air density more uniform.
In a preferred embodiment, a fan is provided at the air inlet to draw air from the air duct.
In this embodiment, the air in the insulation can 3 is inhaled FFU fan filter unit 5 by the fan through the air inlet, filters through high efficiency filter, and the clean air after the filtration is sent out at the uniform velocity, through the refrigeration of control by temperature change structure, 12 disinfection of ultraviolet ray disinfection lamp, get into the wind channel once more, and the fan through the air inlet is with the air suction in insulation can 3, forms the inner loop of clean air in the printing environment to guarantee that biological 3D printing apparatus possesses the controllable clean sterile environment of temperature.
Finally, it is to be noted that: obviously, the above-described embodiments are some, not all embodiments of the present invention, and only illustrate the technical solutions of the present invention, but not limit the same. Based on the embodiments of the present invention, it should be understood by those skilled in the art that modifications can be made to the technical solutions described in the foregoing embodiments, or some or all of the technical features can be equivalently replaced, and the modifications or the replacements do not make the essence of the corresponding technical solutions depart from the scope of the technical solutions of the embodiments of the present invention, and all other embodiments obtained without creative efforts belong to the scope of protection of the present invention.
In the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc., indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and simplicity of description, but do not indicate or imply that the device or element being referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus, should not be construed as limiting the present invention.
In the description of the present invention, it is to be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "connected," and "connected" are to be construed broadly, e.g., as meaning either a fixed connection, a removable connection, or an integral connection; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meanings of the above terms in the present invention can be understood as specific cases by those skilled in the art.
Claims (8)
1. A biological 3D printing apparatus with clean temperature control function that disinfects, printing apparatus includes:
the heat insulation box is arranged on the base, and an air inlet and an air outlet are formed in the heat insulation box;
the printing structure is arranged in the heat insulation box and used for printing the three-dimensional model on the printing platform;
the air outlet of the air filtering structure is communicated with the air inlet of the heat insulation box through an air duct, the air inlet of the air filtering structure is communicated with the air outlet of the heat insulation box, and the air filtering structure is used for sucking air in the heat insulation box for filtering;
the disinfection and sterilization structure is arranged at the top of the heat preservation box and is used for disinfecting and sterilizing air after being cooled, and the air enters the heat preservation box from the air inlet through the air channel;
the printing equipment is characterized by further comprising a temperature control structure, wherein the temperature control structure is arranged between the air outlet of the air filtering structure and the air channel and used for cooling filtered air.
2. The biological 3D printing apparatus with clean sterilization and temperature control functions according to claim 1, wherein the printing structure is disposed on the base, and the heat preservation box is located on the upper portion of the base;
the printing structure includes:
the Y-axis moving part is horizontally arranged on the base, the printing platform is arranged on the Y-axis moving part, and the Y-axis moving part is used for driving the printing platform to move along the Y direction;
the gantry is vertically fixed on the upper part of the base and is positioned on one side of the base;
the X-axis movement part is arranged on the gantry, and the movement direction of the X-axis movement part is vertical to that of the Y-axis movement part;
the Z-axis moving part is arranged on the X-axis moving part, and the moving direction of the Z-axis moving part is vertical to the moving direction of the Y-axis moving part and the moving direction of the X-axis moving part;
the E-axis moving part is arranged on the Z-axis moving part;
and the spray head part is arranged on the Z-axis moving part, a spray head of the spray head part faces the printing platform, and the E-axis moving part is used for controlling the pushing or pulling of a piston in the spray head part.
3. The biological 3D printing equipment with the functions of cleaning, sterilizing and temperature control according to claim 1, wherein the air filtering structure adopts an FFU fan filter unit.
4. The biological 3D printing device with the functions of cleaning, sterilizing and temperature control according to claim 1, wherein the temperature control structure comprises a semiconductor refrigeration sheet and/or an air compressor.
5. The biological 3D printing equipment with the clean sterilization and temperature control functions according to claim 1, wherein the sterilization structure adopts an ultraviolet sterilization lamp, and the ultraviolet sterilization lamp is used for sterilizing bacteria and viruses in the air.
6. The biological 3D printing device with the cleaning, sterilizing and temperature controlling functions as claimed in claim 1, wherein the heat-preserving box further comprises a housing, and the air filtering structure, the temperature controlling structure and the sterilizing and disinfecting structure are all located inside the housing.
7. The biological 3D printing equipment with the functions of cleaning, sterilizing and temperature controlling as claimed in claim 1, wherein air inlets are formed on both sides of the interior of the heat-preservation box.
8. The biological 3D printing equipment with the functions of cleaning, sterilizing and temperature controlling as claimed in claim 1 or 7, wherein a fan is arranged at the air inlet to suck air from the air duct.
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Families Citing this family (4)
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CN110039762B (en) * | 2019-04-10 | 2021-06-15 | 西安理工大学 | Cell/soft tissue 3D printing device with multiple nozzles in cooperation |
CN110039787B (en) * | 2019-05-24 | 2024-04-19 | 杭州捷诺飞生物科技股份有限公司 | 3D printer temperature control system and 3D printer |
CN110936605B (en) * | 2019-11-19 | 2021-07-27 | 华中科技大学 | Biological 3D printing device suitable for gradient structure multi-material |
CN112078134A (en) * | 2020-07-10 | 2020-12-15 | 合肥学院 | Micro-pressure difference-based 3D printing clean environment control device and control method |
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CN105722662A (en) * | 2013-08-01 | 2016-06-29 | 赛多利斯史泰迪生物技术有限责任公司 | Single-use biological 3 dimensional printer |
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CN106671406A (en) * | 2016-12-27 | 2017-05-17 | 扬州大学 | 3D printing device applicable to pneumatic extruding low-temperature platform control |
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