CN109107391B - Filtering device capable of being used under microscope - Google Patents
Filtering device capable of being used under microscope Download PDFInfo
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- CN109107391B CN109107391B CN201811172818.6A CN201811172818A CN109107391B CN 109107391 B CN109107391 B CN 109107391B CN 201811172818 A CN201811172818 A CN 201811172818A CN 109107391 B CN109107391 B CN 109107391B
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D63/00—Apparatus in general for separation processes using semi-permeable membranes
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D65/00—Accessories or auxiliary operations, in general, for separation processes or apparatus using semi-permeable membranes
Abstract
The invention provides a filter device capable of being used under a microscope, which comprises at least one filter single piece, wherein the filter single piece comprises a shell with an inner cavity, and the inner cavity is divided into at least two accommodating cavities which are independent from each other through a filter membrane; the sample in one containing cavity can partially or completely enter the other containing cavity through a filter membrane, the shell is provided with at least one observation window, and the distance from the filter membrane to the objective lens of the microscope through the observation window is not less than one-time focal length of the objective lens of the microscope. By applying the technical scheme of the invention, the structure of the filtering device is simplified, the filtering process of the whole sample can be completely observed under a microscope, the situations of whether the sample is intercepted or not, whether the sample is complete or not and the like in the filtering process of the sample can be known in real time, the filtering condition can be accurately controlled or adjusted, the filtering can be completed under the optimal condition, and the sample can be obtained (or analyzed).
Description
Technical Field
The invention relates to the technical field of filtration, in particular to a filtering device capable of being used under a microscope.
Background
At present, sample analysis is carried out after the whole filtering process is finished, so that people cannot timely and accurately receive real-time conditions in the sample filtering process during filtering, including whether a sample is intercepted or not, whether the sample is complete or not and the like.
The invention patent application No. 200510130411.3 discloses a specimen cassette for an electron microscope for observing a general specimen/living cell, the specimen cassette comprising: a shell, wherein the interior of the shell is provided with a containing chamber, the top and bottom surfaces of the shell are respectively provided with at least one viewing hole which is communicated with the containing chamber and are coaxial relative to each other, and the distance between the bottom end of the viewing hole positioned on the top surface of the shell and the top end of the viewing hole positioned on the bottom surface of the shell is less than 50 mu m; the chamber has extremely thin thickness, even if the liquid sample is injected into the chamber, the electron beam of the electron microscope can not penetrate through the chamber, and the chamber can be observed; and can be used for placing general samples or living cells, and can be matched with an electron microscope for observation.
The invention application No. 200510130413.2 discloses a closed observation environment for an electron microscope, comprising: the shell is internally provided with a containing chamber, and the top and bottom surfaces of the shell are respectively provided with at least one viewing hole which is communicated with the containing chamber and are coaxial relative to each other; each viewing hole is sealed with a film; therefore, a common sample or living cells can be placed in the chamber to cooperate with an electron microscope for observation. And the liquid or the gas can be sealed in the shell, so that the problem that the liquid or the gas can escape outwards is solved.
Both of the above devices can realize the observation of a sample or living cells under an electron microscope, but still cannot realize the observation of the whole process or dynamic change of the filtration of the sample or living cells (the cells are kept active and do not need special means such as fixation) under an optical microscope.
Therefore, it is important to design a filtering apparatus capable of knowing the microscopic real-time condition of the sample during filtering in real time, so as to precisely control or adjust the filtering condition, complete the filtering under the optimal condition and obtain (or analyze) the sample.
Disclosure of Invention
The invention aims to provide a filtering device which is simple in structure and can be used for filtering samples observed in real time under a microscope, and the specific scheme is as follows:
a filter device which can be used under a microscope comprises at least one filter single piece, and can observe the filtration process of a sample in the filter single piece under the microscope;
the filtering single piece comprises a shell with an inner cavity, the inner cavity is divided into at least two containing cavities which are mutually independent through a filter membrane, and a sample in one containing cavity can partially or completely enter the other containing cavity through the filter membrane; the shell is provided with at least one observation window, and the distance from the filter membrane to the objective lens of the microscope through the observation window is not less than one-time focal length of the objective lens of the microscope, so that a sample on the filter membrane can be conveniently observed and imaged under the microscope.
Preferably, in the above solution, the thickness of the filtering single piece is less than 20mm, preferably less than 15mm, and more preferably less than 10 mm.
Preferably, in the above technical solution, the minimum distance from the filter membrane to the objective lens of the microscope through the observation window is between one-time focal length and two-time focal length of the objective lens of the microscope, and preferably, the minimum distance from the filter membrane to the objective lens of the microscope through the observation window is greater than the one-time focal length of the objective lens of the microscope and infinitely close to the one-time focal length.
In the above technical solution, preferably, when a plurality of filtering single pieces are used in combination, the number of the filtering single pieces is at least two, and the plurality of filtering single pieces are at least one of arranged in series, arranged in parallel or arranged independently.
Preferably, in the above technical solution, the filtering unit includes a cover and a base, and the cover and the base are detachably connected;
the base comprises a base body, a base inner cavity and a first circulation port, and the base inner cavity and the first circulation port are both arranged on the base body; the cover body comprises a cover body, a cover body inner cavity and a second circulation port, and the cover body inner cavity and the second circulation port are both arranged on the cover body; clamping parts for clamping the filtering membrane are arranged on the inner wall of the inner cavity of the base and the inner wall of the inner cavity of the cover body, so that the inner cavity formed by the inner cavity of the base and the inner cavity of the cover body is divided into a first accommodating cavity and a second accommodating cavity which are independent of each other by the filtering membrane, the first circulation port is communicated with the first accommodating cavity, and the second circulation port is communicated with the second accommodating cavity;
the sample entering the second containing cavity through the second through hole can partially or completely enter the first containing cavity through the filter membrane for filtering, and the filtrate in the first containing cavity is discharged from the first through hole; alternatively, the sample entering the first receiving chamber through the first flow port can partially or completely enter the second receiving chamber through the filter membrane for filtration, and the filtrate in the second receiving chamber is discharged through the second flow port.
Preferred among the above technical scheme, the lower extreme tip of base and the upper end tip of lid at least one is equipped with the observation window that the light transmissivity is good, and light can penetrate into and reach filtration membrane from the observation window, or light sends from filtration membrane and can penetrate through the observation window, satisfies the formation of image needs. Preferably, all be equipped with the observation window that the light transmissivity is good on the base with on the lid, just observation window on the base with observation window on the lid sets up correspondingly, and light can penetrate from an observation window, reaches and passes behind the filtration membrane, penetrates from another observation window, satisfies the formation of image demand.
Preferably, in the above technical solution, the observation window is an observation window having a flat surface;
the observation window is made of a material with good light transmittance, or the base, the cover body and the observation window are made of materials with good light transmittance;
the material with good light transmittance refers to a material with light transmittance of 50-100%, and is preferably at least one of glass, silica, polyvinyl chloride, polystyrene, polycarbonate, polymethyl methacrylate and methyl methacrylate.
Preferably, in the above technical scheme, the upper end of the inner cavity of the base is provided with an internal thread, and the lower end of the cover body is provided with an external thread matched with the internal thread;
or the base body and the cover body are connected through at least one clamping tenon.
Preferably, in the above technical scheme, the inner cavity of the base is provided with a circular boss facing the center along the circumferential direction, and the boss and the inner cavity of the base form a concave structure together; the boss is used for placing a filtering membrane;
the lower extreme of lid body is equipped with the annular card and holds the platform, the card hold the platform with the boss cooperation realizes filtration membrane's fixed seal.
Preferably among the above technical scheme, still including the position that is used for setting up the sealing washer on the boss, through the platform is held to the card the boss and sealing washer cooperation realize filtration membrane's fixed seal.
By applying the technical scheme of the invention, the effects are as follows:
1. the invention discloses a filtering device capable of being used under a microscope, which comprises at least one filtering single piece, wherein the filtering single piece comprises a shell with an inner cavity, the inner cavity is divided into at least two containing cavities which are independent from each other through a filtering membrane, and the structure is simplified; the sample in one holding cavity can partially or completely enter the other holding cavity through the filter membrane, the shell is provided with at least one observation window, the distance from the filter membrane to the objective lens of the microscope through the observation window is not less than one-time focal length of the objective lens of the microscope, the whole filtering process of the sample can be completely observed under the microscope, the conditions of whether the sample is intercepted or not, whether the sample is complete or not and the like in the filtering process of the sample can be known in real time, the filtering condition can be accurately controlled or adjusted, and the filtering can be completed and the sample can be obtained (or analyzed) under the optimal condition.
2. The thickness of the filter element according to the invention is less than 20mm (e.g. 0.01-20mm), preferably less than 15mm (e.g. 2-15mm), more preferably less than 10mm (e.g. 5-8 mm). The thickness of the filtering unit is designed to be capable of being imaged under a microscope and the sample is filtered smoothly, and the thickness of the filtering unit cannot be too thick (too thick is not beneficial to imaging under the microscope).
3. The minimum distance from the filter membrane to the objective lens of the microscope through the observation window is between one focal length and two focal lengths of the objective lens of the microscope, and preferably, the minimum distance from the filter membrane to the objective lens of the microscope through the observation window is larger than one focal length of the objective lens of the microscope and infinitely close to one focal length. Clear imaging can be realized, and the observation precision is improved.
4. When a plurality of filtering single pieces are used in combination, the number of the filtering single pieces is at least two, and the filtering single pieces are at least one of arranged in series, in parallel or independently, and can be selected according to actual requirements. A plurality of groups of samples are filtered in the same batch, and a plurality of filtering single pieces can be arranged in parallel or independently; the sample is filtered in different levels (different filtering membranes are arranged), a plurality of filtering single pieces can be arranged in a serial combination and parallel mode, and the like.
5. The filtering single piece can be integrally formed by the shell and the filtering membrane, and a filtering test (the filtering membrane cannot be replaced) can be carried out once or for a limited number of times. The filtering single piece can also adopt a structure that the detachable base and the detachable cover body are combined, so that the replacement requirements of local parts such as the filtering membrane and the like are met. The combined design of the base and the cover body can realize intermittent filtration observation and continuous sample filtration observation, and different requirements are met. The base and the cover can be detachably connected in various ways, and the detachable connection mode is preferably as follows: the upper end of the inner cavity of the base is provided with an internal thread, and the lower end of the cover body is provided with an external thread matched with the internal thread; or the base body and the cover body are connected through at least one clamping tenon. The processing is convenient, and the installation and the dismantlement are convenient.
6. At least one of the lower end part of the base and the upper end part of the cover body is provided with an observation window with good light transmission, and the effects are as follows: the selection is carried out according to actual requirements, and respective requirements of the inverted microscope and the upright microscope can be met; only one observation window is arranged to meet the observation requirement of a self-luminous sample (such as a fluorescent sample); two or more than two observation windows are arranged, so that the observation requirement of the non-luminous sample can be met, and particularly: when the observation window on the base, the observation window on the lid and the filtration membrane three set up correspondingly, more preferably the three sets up with the central axis, and light can penetrate from an observation window, reachs and passes behind the filtration membrane, penetrates from another observation window.
7. The observation window provided by the invention adopts the observation window with the flat surface, so that the observation window is convenient to be combined with a microscope for use. The invention has two modes: firstly, the base body, the cover body and the observation window are made of materials with good light transmittance, preferably, the base and the observation window thereon are of an integrated structure, and the cover and the observation window thereon are of an integrated structure; in the second type, only the observation window is made of a material having good light transmittance (that is, both the observation window on the base and the observation window on the cover are made of a material having good light transmittance). The method can be selected according to actual requirements, and the requirement that the filtering process of a sample in a filtering single piece can be observed under a microscope is met.
8. The filter membrane of the invention can be arranged in a plurality of ways in the single filter piece, and the following is preferred: the inner cavity of the base is provided with a circular boss facing the center along the circumferential direction, and the boss and the inner cavity of the base can form a concave structure together; the boss is used for arranging (mainly playing a role in supporting and placing) the filtering membrane; the lower extreme of lid body is equipped with the annular card and holds the platform, the card hold the platform with the boss cooperation realizes filtration membrane's fixed seal. The arrangement of the filtering membrane in the filtering single piece is convenient to operate and filter. More preferably here: still including being used for supporting the position of placing the sealing washer on the boss, through the platform is held to the card the boss and sealing washer cooperation realizes filtration membrane's fixed seal, can make the second hold the chamber or the first sample that holds the chamber can only get into first holding chamber or second holding chamber through filtering part or whole, realizes filtering.
9. The material with good light transmittance is a material with light transmittance of more than or equal to 50% (such as 50% -100%), preferably, the material with light transmittance of more than or equal to 70% (such as 70% -100%), more preferably, the material with light transmittance of more than or equal to 80% (such as 80% -100%), and can be determined according to actual requirements to meet the observation requirements of samples.
In addition to the objects, features and advantages described above, other objects, features and advantages of the present invention are also provided. The present invention will be described in further detail below with reference to the accompanying drawings.
Drawings
The accompanying drawings, which are incorporated in and constitute a part of this application, illustrate embodiments of the invention and, together with the description, serve to explain the invention and not to limit the invention. In the drawings:
FIG. 1 is a schematic view showing the overall structure of a filtration apparatus usable under a microscope in example 1;
FIG. 2 is a schematic cross-sectional view of the base of FIG. 1;
FIG. 3 is a schematic cross-sectional view of the cover of FIG. 1;
FIG. 4 is a diagram showing the whole process of filtration of human lung adenocarcinoma cell HCC827 under microscope in example 1;
wherein: 1. the filtering single piece comprises 1.1 parts of a filtering single piece, a base, 1.11 parts of a base body, 1.12 parts of a base inner cavity, 1.13 parts of a first circulation port, 1.14 parts of a boss, A parts of an internal thread, 1.2 parts of a cover body, 1.21 parts of a cover body, 1.22 parts of a cover body inner cavity, 1.23 parts of a second circulation port, 1.24 parts of a clamping table, B parts of an external thread, 2 parts of a filtering membrane.
Detailed Description
Embodiments of the invention will be described in detail below with reference to the drawings, but the invention can be implemented in many different ways, which are defined and covered by the claims.
Example 1:
a filter device which can be used under a microscope comprises a filter single piece 1, a filter device and a control device, wherein the filter single piece can be used for observing the filtration process of a sample in the filter single piece under the microscope; the filtering single piece comprises a shell with an inner cavity, the inner cavity is divided into two containing cavities which are arranged in parallel and are mutually independent through a filter membrane, and a sample in one containing cavity can partially or completely enter the other containing cavity through the filter membrane; the shell is provided with at least one observation window, and the distance from the filter membrane to the objective lens of the microscope through the observation window is not less than one-time focal length of the objective lens of the microscope, so that a sample on the filter membrane can be conveniently observed and imaged under the microscope. The method comprises the following steps:
the minimum distance from the filter membrane to the objective of the microscope through the observation window is between one focal length and two focal lengths of the objective of the microscope, and preferably: the minimum distance from the filtering membrane to the objective lens of the microscope through the observation window is larger than one-time focal length of the objective lens of the microscope and is infinitely close to one-time focal length, clear imaging can be achieved, and observation accuracy is improved.
Further preferably, said filtering single-piece 1 comprises a base 1.1 and a cover 1.2, see in detail fig. 1, said base and said cover being removably connected, as detailed below:
the base 1.1 includes a base body 1.11, a base inner cavity 1.12 and a first circulation port 1.13, see fig. 2 in detail, the base inner cavity 1.12 and the first circulation port 1.13 are both disposed on the base body.
The cover 1.2 comprises a cover body 1.21, a cover interior 1.22 and a second flow opening 1.23, see fig. 3 in detail, which are both arranged on the cover body.
On the inner wall of base inner chamber with all be equipped with the card that is used for the card to hold filtering membrane and hold the position for filtering membrane will the base inner chamber with the inner chamber that the lid inner chamber is constituteed falls into and sets up side by side and mutually independent first holds chamber and second and holds the chamber, and second circulation mouth holds the chamber intercommunication with the second, and first circulation mouth and first hold the chamber intercommunication, here: the sample entering the second containing chamber through the second through hole can partially or completely enter the first containing chamber through the filter membrane for filtering, and the filtrate in the first containing chamber is discharged from the first through hole. Preferably, the inner cavity 1.12 of the base is provided with a circular boss 1.14 facing to the center along the circumferential direction, and the boss and the inner cavity of the base form a concave structure together; the boss is used for supporting and placing the filter membrane 2; the lower end of the cover body 1.21 is provided with a circular clamping table 1.24, and the clamping table and the free end of the boss are matched to fix the filtering membrane 2. In addition, the boss further comprises a part for supporting and placing a sealing ring (not shown), and the filtering membrane is fixedly sealed through the matching of the clamping table, the boss and the sealing ring (preferably, at least one of the clamping table and the filtering membrane and the boss and the filtering membrane is provided with the sealing ring).
The lower extreme tip of base and the upper end tip of lid all is equipped with the observation window that has the planishing face and the light transmissivity is good, and observation window on the base, the observation window on the lid and filtration membrane three set up with the central axis. The base, lid and observation window are the material that the light transmissivity is good, and the material that the light transmissivity is preferred to be more than 90% of the material that the light transmissivity is good, such as polycarbonate. Preference is given here to: the base and the observation window on the base as well as the cover and the observation window on the cover are all of an integrated structure.
The upper end of the base inner cavity is provided with an internal thread A, the lower end of the cover body is provided with an external thread B matched with the internal thread A, the detachable connection of the base and the cover body is realized through the internal thread A on the base and the external thread B on the cover body, and the operation is convenient.
The thickness of the filter element according to the invention is 10mm, as shown in fig. 1H, excluding the height of the second flow opening.
The technical scheme of the embodiment is specifically as follows:
placing the filter membrane 2 on a boss 1.14 of the inner cavity 1.12 of the base; placing the sealing ring on the filtering membrane on the boss 1.14; the size of the sealing ring is matched with the boss and cannot be larger than the boss; the base and the cover body are screwed down through the matching of the internal thread and the external thread until the end part of the lower end of the clamping table 1.24 is contacted with the filtering membrane on the boss.
Placing the assembled filter units under a corresponding microscope; injecting the sample from the second flow port 1.23 in the cover 1.2; the sample enters the second accommodating cavity, and is filtered by the filter membrane, and the filtrate enters the first accommodating cavity; filtrate in the first receiving chamber is discharged from the first through-flow opening 1.13. The whole process of sample filtration is carried out under the microscope condition, the microscopic real-time conditions (such as whether the sample is trapped or not, whether the sample is complete or not) during sample filtration can be known in real time, the filtration condition can be accurately controlled or adjusted, and the filtration is completed under the optimal condition to obtain (or analyze) the sample.
Fig. 4 shows the filtering device of this embodiment used to filter human lung adenocarcinoma cells HCC827, and the whole filtering process is monitored by shooting under a fluorescence microscope, and the experimental process is as follows: human lung adenocarcinoma cell HCC827 is stably transfected with GFP gene, and can continuously and stably express GFP (green fluorescent protein) as a tracer. GFP emits a bright green light when excited by 488nm light. Adding 1X 10 of normal saline into 120 ml of normal saline4GFP-HCC827 cells, a pipeline and a peristaltic pump are installed, a track etching membrane with the aperture of 8 microns is arranged in a filtering device, the whole filtering device is placed in an inverted fluorescence microscope, and filtering is carried out at the flow rate of 50 ml/min; switching between white field light and fluorescent field the entire filtration process was observed under the microscope and video was taken (see details in the A-P process in FIG. 4).
As can be seen from fig. 4: a, G, K and P in FIG. 4 are white field light fields, and B, C, D, E, F, H, I, J, L, M, N and O in FIG. 4 are fluorescence fields (50 μm for both scales B and F in FIG. 4).
In the A-M process of FIG. 4: over time, it was observed that more and more GFP-HCC827 cells were trapped on the filter membrane, with intact cell morphology and green fluorescence.
In the N-P process of FIG. 4: when the filtration was continued for 147 seconds, the sample liquid was completely filtered, air was introduced into the filter unit, and the cells were swollen and ruptured.
It is fully explained through this experiment that the filter equipment of this embodiment can effectively observe whole filtering process to the special event that takes place among the filtering process carries out effective monitoring.
The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention, and various modifications and changes may be made by those skilled in the art. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (12)
1. A filtering method for real-time understanding of microscopic real-time conditions of sample filtration under a microscope, comprising the steps of:
step one, placing the assembled filtering single piece in the filtering device under a corresponding microscope;
wherein, the filtering device comprises at least one filtering single piece, and the filtering process of the sample in the filtering single piece can be observed under a microscope;
the filtering single piece comprises a shell with an inner cavity, the inner cavity is divided into at least two containing cavities which are mutually independent through a filter membrane, and a sample in one containing cavity can partially or completely enter the other containing cavity through the filter membrane; the shell is provided with at least one observation window, and the distance from the filter membrane to the objective lens of the microscope through the observation window is not less than one-time focal length of the objective lens of the microscope, so that a sample on the filter membrane can be observed and imaged under the microscope; the filtering unit comprises a cover body and a base, and the cover body is detachably connected with the base; the base comprises a base body, a base inner cavity and a first circulation port, and the base inner cavity and the first circulation port are both arranged on the base body; the cover body comprises a cover body, a cover body inner cavity and a second circulation port, and the cover body inner cavity and the second circulation port are both arranged on the cover body; clamping parts for clamping the filtering membrane are arranged on the inner wall of the inner cavity of the base and the inner wall of the inner cavity of the cover body, so that the inner cavity formed by the inner cavity of the base and the inner cavity of the cover body is divided into a first accommodating cavity and a second accommodating cavity which are independent of each other by the filtering membrane, the first circulation port is communicated with the first accommodating cavity, and the second circulation port is communicated with the second accommodating cavity; the upper end of the inner cavity of the base is provided with an internal thread, and the lower end of the cover body is provided with an external thread matched with the internal thread; at least one of the lower end part of the base and the upper end part of the cover body is provided with an observation window; the observation window is made of a material with good light transmittance; the material with good light transmittance refers to the material with light transmittance of 50-100%;
and secondly, injecting a sample from a second circulation port in the cover body, enabling the sample entering the second accommodating cavity through the second circulation port to partially or completely enter the first accommodating cavity through the filtering membrane for filtering, and discharging the filtrate in the first accommodating cavity from the first circulation port.
2. The filtration method according to claim 1, wherein: the thickness of the filtering single piece is less than 20 mm.
3. The filtration method according to claim 2, wherein: the thickness of the filtering single piece is less than 15 mm.
4. A method of filtering according to claim 3, characterized in that: the thickness of the filtering single piece is less than 10 mm.
5. The filtration method according to claim 1, wherein: the minimum distance from the filter membrane to the objective lens of the microscope through the observation window is between one focal length and two focal lengths of the objective lens of the microscope.
6. The filtration method according to claim 5, wherein: the minimum distance from the filter membrane to the objective lens of the microscope through the observation window is larger than one focal length of the objective lens of the microscope and infinitely approaches to one focal length.
7. The filtration method according to claim 1, wherein: when the filtering single pieces are used in a combined mode, the number of the filtering single pieces is at least two, and the filtering single pieces are at least one of arranged in series, arranged in parallel or arranged independently.
8. The filtration method according to claim 1, wherein: the base with all be equipped with the observation window that the light transmissivity is good on the lid, just observation window on the base with corresponding the setting of observation window on the lid, light can jet into from an observation window, reaches and passes behind the filtration membrane, jets out from another observation window.
9. The filtration method according to claim 8, wherein: the observation window is an observation window with a flat surface; the base, the cover body and the observation window are all made of materials with good light transmittance.
10. The filtration method according to claim 9, wherein: the material with good light transmittance is at least one of glass, silicon dioxide, polyvinyl chloride, polystyrene, polycarbonate, polymethyl methacrylate and methyl methacrylate.
11. The filtration method according to claim 1, wherein: the inner cavity of the base is provided with a circular boss facing the center along the circumferential direction; the boss is used for placing a filtering membrane;
the lower extreme of lid body is equipped with the annular card and holds the platform, the card hold the platform with the boss cooperation realizes filtration membrane's fixed seal.
12. The filtration method according to claim 11, wherein: still including the position that is used for setting up the sealing washer on the boss, through the card is held the platform the boss and sealing washer cooperation realize filtration membrane's fixed seal.
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103933780A (en) * | 2014-04-01 | 2014-07-23 | 西安交通大学 | Real-time dynamic observation system for fiber filtering |
| CN104039948A (en) * | 2012-01-05 | 2014-09-10 | 日立化成株式会社 | Cell Trapping Device |
| CN106164240A (en) * | 2014-03-27 | 2016-11-23 | 日立化成株式会社 | Cell capture device, cell capture filter, cell capture device and the manufacture method of cell capture device |
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| WO2013043122A1 (en) * | 2011-09-19 | 2013-03-28 | Nanyang Technological University | A reinforced filter with a metallic filtering layer |
| CN103937658B (en) * | 2014-03-28 | 2015-11-04 | 武汉介观生物科技有限责任公司 | A kind of rare cell detection chip and application thereof |
| WO2015146682A1 (en) * | 2014-03-28 | 2015-10-01 | シャープ株式会社 | Filtration tool |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104039948A (en) * | 2012-01-05 | 2014-09-10 | 日立化成株式会社 | Cell Trapping Device |
| CN106164240A (en) * | 2014-03-27 | 2016-11-23 | 日立化成株式会社 | Cell capture device, cell capture filter, cell capture device and the manufacture method of cell capture device |
| CN103933780A (en) * | 2014-04-01 | 2014-07-23 | 西安交通大学 | Real-time dynamic observation system for fiber filtering |
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