CN109107391A - A kind of filter device that can be used under the microscope - Google Patents
A kind of filter device that can be used under the microscope Download PDFInfo
- Publication number
- CN109107391A CN109107391A CN201811172818.6A CN201811172818A CN109107391A CN 109107391 A CN109107391 A CN 109107391A CN 201811172818 A CN201811172818 A CN 201811172818A CN 109107391 A CN109107391 A CN 109107391A
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- Prior art keywords
- lid
- filter membrane
- microscope
- sample
- filter
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D63/00—Apparatus in general for separation processes using semi-permeable membranes
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D65/00—Accessories or auxiliary operations, in general, for separation processes or apparatus using semi-permeable membranes
Abstract
The present invention provides a kind of filter device that can be used under the microscope, including at least one filtering single-piece, filtering single-piece include a shell with inner cavity, and inner cavity is separated into mutually independent at least two accommodating chamber through filter membrane；Sample in one accommodating chamber can partly or entirely enter another accommodating chamber through filter membrane, and the shell has at least one observation window, and the filter membrane is it is observed that window is not less than one times of focal length of micro objective to the distance of microscopical object lens.It applies the technical scheme of the present invention, filter apparatus configuration is simplified, and it is capable of the filter process of the entire sample of complete observation under the microscope, in real time understand sample filtering during sample whether there is or not retention, whether sample complete situations such as, convenient for being accurately controlled or adjusting filter condition, complete to filter and obtain (or analysis) sample with optimal conditions.
Description
Technical field
The present invention relates to filtration arts, particularly, are related to a kind of filter device that can be used under the microscope.
Background technique
Currently, sample analysis is analyzed again after the completion of entire filter process, this make people in filtering without
Whether the real-time condition connect during sample filtering of method promptly and accurately, including sample are complete etc. whether there is or not retention, sample.
Application No. is 200510130411.3 patents of invention to disclose a kind of observing general sample/
Electron microscope sample box, this sample box includes: a shell, and inside has a room, and the top bottom surface of the shell respectively has
An at least observation hole is connected to the room and coaxial opposite to each other, the observation hole bottom end positioned at the housing top surface be located at the shell
The observation hole top of bottom surface is at a distance of less than 50 μm；There is very thin thickness by this room, even if internal injection fluid sample,
Also will not make the electron beam of electron microscope can not penetrate, and then for observation；And for being placed in general sample or active somatic cell,
By being observed with the sub- microscope of cooperated power supply.
Application No. is 200510130413.2 patent applications to disclose a kind of closed observing ring of electron microscope
Border includes: a shell, and inside has a room, and respectively there is the top bottom surface of the shell an at least observation hole to be connected to the room
And it is coaxial opposite to each other；Respectively observation hole envelope sets a film；It can be built into general sample or active somatic cell in the room as a result,
It is observed with the sub- microscope of cooperated power supply.And liquid or gas can be encapsulated in the shell, solving liquid or gas may be to
The problem of outer loss.
Above two device can be realized observing samples under an electron microscope or active somatic cell, but still cannot achieve
Sample or living cells (cell be to maintain active, without any special measures processing such as fixed) filtering is observed under an optical microscope
Whole process or dynamic change.
Therefore, the filter device for designing a kind of microcosmic real-time condition when can understand sample filtering in real time, can be accurate
Ground control or adjustment filter condition are completed to filter and obtain (or analysis) sample with optimal conditions, be of great significance.
Summary of the invention
It is simplified the purpose of the present invention is to provide a kind of structure and can be used to observe sample under microscope in real time and be filtered
Filter device, concrete scheme is as follows:
A kind of filter device that can be used under the microscope, including at least one filtering single-piece, can observe under the microscope
Filter process of the sample in filtering single-piece；
The filtering single-piece includes a shell with inner cavity, and inner cavity is separated into mutually independent at least two through filter membrane
Accommodating chamber, the sample in an accommodating chamber can partly or entirely enter another accommodating chamber through filter membrane；The shell has extremely
A few observation window, the filter membrane is it is observed that window is not less than one times of coke of micro objective to the distance of microscopical object lens
Away from observing imaging under the microscope convenient for the sample on filter membrane.
Preferred in above technical scheme, the thickness of the filtering single-piece is less than 20mm, preferably smaller than 15mm, more preferably small
In 10mm.
Preferred in above technical scheme, the filter membrane is it is observed that the minimum range of window to microscopical object lens is located at shows
One times of focal length of objective between two focus length, the preferably described filter membrane it is observed that window to microscopical object lens most narrow spacing
From one times of focal length for being greater than micro objective and it is infinitely close to one times of focal length.
Preferred in above technical scheme, the filtering single-piece is multiple when being used in combination, and the quantity for filtering single-piece is extremely
It is less two, multiple filtering single-pieces are at least one of to connect, be arranged side by side or independently of each other between each other.
Preferred in above technical scheme, the filtering single-piece includes lid and pedestal, and the lid and the pedestal can
It is detachably connected with；
The pedestal includes base body, base intracavity and the first communication port, the base intracavity and described first-class
Port is arranged on the base body；The lid includes lid ontology, lid inner cavity and the second communication port, the lid
Intracoelomic cavity and second communication port are arranged on the lid ontology；On the inner wall of the base intracavity and in the lid
The fastening part for clamping filter membrane is equipped on the inner wall of chamber, so that filter membrane will be in the base intracavity and the lid
The inner cavity of chamber composition is divided into mutually independent first accommodating chamber and the second accommodating chamber, and first communication port and described first accommodate
Chamber connection, the second communication port are connected to the second accommodating chamber；
The sample entered in the second accommodating chamber through the second communication port can partly or entirely enter described the through the filter membrane
One accommodating chamber is filtered, and the filtrate in the first accommodating chamber is discharged from the first communication port；Either, enter the through the first communication port
Sample in one accommodating chamber can be partly or entirely filtered into second accommodating chamber through the filter membrane, the second accommodating chamber
In filtrate be discharged from the second communication port.
It is preferred in above technical scheme, the upper end end of the lower end of the pedestal and the lid at least one
Equipped with the good observation window of translucency, light can be injected from observation window and reach that filter membrane or light issues from filter membrane can
With it is observed that window projects, needs are imaged in satisfaction.It is preferred that on the pedestal and it is equipped with the good observation window of translucency on the lid,
And the corresponding setting of observation window on the observation window and the lid on the pedestal, light can be injected from an observation window, be arrived
After reaching and passing through the filter membrane, is projected from another observation window, meet imaging demand.
Preferred in above technical scheme, the observation window is the observation window with burnishing surface；
The material of the observation window is the good material of translucency or the pedestal, the lid and the observation
The material of window three is the good material of translucency；
The good material of translucency refer to light transmittance be 50-100% material, preferably glass, silica, polyvinyl chloride,
At least one of polystyrene, polycarbonate, polymethyl methacrylate and methyl methacrylate.
Preferred in above technical scheme, the upper end of the base intracavity is equipped with internal screw thread, the lower end of the lid ontology
Equipped with external screw thread matched with the internal thread；
Either, it is connected between the base body and the lid ontology by an at least trip.
Preferred in above technical scheme, the base intracavity is along the circumferential direction equipped with towards the convex of the circular ring shape at center
Platform, the boss and base intracavity are formed together concave structure；The boss is for placing filter membrane；
The lower end of the lid ontology is equipped with the clamping platform of circular ring shape, and filtering is realized in the clamping platform and boss cooperation
The fixing seal of film.
It is preferred in above technical scheme, further include the position for sealing ring to be arranged on the boss, passes through the card
Hold the fixing seal that filter membrane is realized in platform, the boss and sealing ring cooperation.
It applies the technical scheme of the present invention, effect is:
1, the filter device disclosed by the invention that can be used under the microscope includes at least one filtering single-piece, filters single-piece
Shell including one with inner cavity, inner cavity are separated into mutually independent at least two accommodating chamber through filter membrane, and structure is simplified；One
Sample in accommodating chamber can partly or entirely enter another accommodating chamber through filter membrane, and the shell is at least one observation
Window, the filter membrane, can be aobvious it is observed that window is not less than one times of focal length of micro objective to the distance of microscopical object lens
The filter process of the entire sample of complete observation under micro mirror, in real time understand sample filtering during sample whether there is or not retention, sample whether
Situations such as complete, completes to filter and obtain (or analysis) sample with optimal conditions convenient for being accurately controlled or adjusting filter condition
Product.
2, the thickness of heretofore described filtering single-piece is less than 20mm (such as 0.01-20mm), preferably smaller than 15mm (such as 2-
15mm), more preferably less than 10mm (such as 5-8mm).The thickness design of filtering single-piece wants to be imaged under the microscope and sample is suitable
It, cannot be too thick (too thick to be unfavorable for being imaged under the microscope) subject to benefit is filtered.
3, heretofore described filter membrane is it is observed that the minimum range of window to microscopical object lens is located at micro objective
One times of focal length is between two focus length, and the preferably described filter membrane filter membrane is it is observed that the minimum range of window to microscopical object lens is big
In micro objective one times of focal length and be infinitely close to one times of focal length.It can be realized clear imaging, improve the precision of observation.
4, heretofore described filtering single-piece is multiple when being used in combination, and filters the quantity at least two of single-piece, Duo Gesuo
Stating filtering single-piece between each other is at least one of to connect, be arranged side by side or independently of each other, can be selected according to actual needs.Together
Batch filters multiple groups sample, and multiple filtering single-pieces can be used and be set side by side or be arranged independently of each other；Sample carries out different stage
It filters (different filter membranes is set), multiple filtering single-piece series combination juxtaposition settings etc. can be used.
5, shell can be used in filtering single-piece in the present invention and filter membrane is integrally formed, and carries out single or limited amount time mistake
Filter test (non-exchange filter membrane).The structure that detachable pedestal and lid can also be used to be combined filtering single-piece,
Meet the replacement demand of the component homes such as filter membrane.Batch filtration observation can be realized in the Combination Design of pedestal and lid,
The observation that sample continuous filtration process can be achieved, meets the needs of different.The detachable connection of pedestal and lid in the present invention
There are many modes, herein preferably: the upper end of the base intracavity is equipped with internal screw thread, the lower end of the lid ontology be equipped with it is described
The external screw thread that internal screw thread matches；Either, it is connected between the base body and the lid ontology by an at least trip.
It is easy to process, and mount and dismount conveniently.
6, the upper end end of the lower end of heretofore described pedestal and the lid at least one be equipped with translucency
Good observation window, effect is: selecting according to actual needs, can meet inverted microscope and just set microscopical respective demand；Only
An observation window, which is arranged, can satisfy the observation needs of self-luminous sample (such as fluorescent samples)；Two or more observations is set
Window can satisfy the observation needs of not luminescent sample, especially: observation window on observation window, lid and filtering on pedestal
When the corresponding setting of film three, more preferable three is arranged with central axis, and light can be injected from an observation window, reach and pass through
After the filter membrane, projected from another observation window.
7, observation window uses the observation window equipped with burnishing surface in the present invention, convenient for being applied in combination with microscope.The present invention has
Two ways: the first, base body, lid ontology and observation window are the good material of translucency, preferably pedestal and thereon
Observation window be integrated, lid and observation window thereon are integrated；Second, only observation window is that translucency is good
Material (both observation windows on observation window and lid i.e. on pedestal are the good material of translucency).It can be according to actual needs
Selection meets the filter process that can observe sample under the microscope in filtering single-piece.
8, set-up mode of the filter membrane in filtering single-piece is a variety of in the present invention, and herein preferably: the base intracavity is along circle
Circumferential direction is equipped with the boss towards the circular ring shape at center, and boss and base intracavity can form concave structure together；The boss is used
In setting (primarily serving the effect of support and placement) filter membrane；The lower end of the lid ontology is equipped with the clamping platform of circular ring shape,
The fixing seal of filter membrane is realized in the clamping platform and boss cooperation.Setting operation side of the filter membrane in filtering single-piece
Just, it is convenient for filtering.Herein more preferably: further including being used to support the position for placing sealing ring on the boss, by described
The fixing seal of filter membrane is realized in clamping platform, the boss and sealing ring cooperation, enables to the second accommodating chamber or the first appearance
The sample of chamber received is only capable of through filtration fraction or fully enters the first accommodating chamber or the second accommodating chamber, realizes filtering.
9, the good material of translucency is material (such as 50%-100%) of the light transmittance more than or equal to 50% in the present invention, preferably
Light transmittance is more than or equal to 70% (such as 70%-100%), and more preferable light transmittance is more than or equal to 80% (such as 80%-100%), may be used also
To be determined according to actual needs, meet the observation needs of sample.
Other than objects, features and advantages described above, there are also other objects, features and advantages by the present invention.
Below with reference to accompanying drawings, the present invention is described in further detail.
Detailed description of the invention
The attached drawing constituted part of this application is used to provide further understanding of the present invention, schematic reality of the invention
It applies example and its explanation is used to explain the present invention, do not constitute improper limitations of the present invention.In the accompanying drawings:
Fig. 1 is the overall structure diagram for the filter device that can be used under the microscope in embodiment 1；
Fig. 2 is the schematic cross-sectional view of pedestal in Fig. 1；
Fig. 3 is the schematic cross-sectional view of lid in Fig. 1；
Fig. 4 is that microscopically observation is filtered overall process figure to human lung adenocarcinoma cell HCC827 in embodiment 1；
Wherein: 1, filtering single-piece, 1.1, pedestal, 1.11, base body, 1.12, base intracavity, the 1.13, first communication port,
1.14, boss, A, internal screw thread, 1.2, lid, 1.21, lid ontology, 1.22, lid inner cavity, the 1.23, second communication port, 1.24,
Clamping platform, B, external screw thread, 2, filter membrane.
Specific embodiment
The embodiment of the present invention is described in detail below in conjunction with attached drawing, but the present invention can be limited according to claim
Fixed and covering multitude of different ways is implemented.
Embodiment 1:
A kind of filter device that can be used under the microscope, including a filtering single-piece 1, can observe sample under the microscope
Filter process in filtering single-piece；The filtering single-piece includes a shell with inner cavity, and inner cavity is separated into simultaneously through filter membrane
Column are arranged and mutually independent two accommodating chambers, and the sample in an accommodating chamber can partly or entirely enter another through filter membrane
Accommodating chamber；The shell has at least one observation window, and the filter membrane is not it is observed that the distance of window to microscopical object lens is small
In one times of focal length of micro objective, imaging is observed under the microscope convenient for the sample on filter membrane.Specifically:
The filter membrane is it is observed that the minimum range of window to microscopical object lens is located at one times of focal length of micro objective extremely
Between two focus length, preferably: the filter membrane filter membrane is it is observed that the minimum range of window to microscopical object lens is greater than microscope
One times of focal length of object lens and it is infinitely close to one times of focal length, can be realized clear imaging, improve the precision of observation.
Further preferably, the filtering single-piece 1 includes pedestal 1.1 and lid 1.2, is detailed in Fig. 1, the pedestal and the lid
Body detachable connection, Details as Follows:
The pedestal 1.1 includes base body 1.11, base intracavity 1.12 and the first communication port 1.13, is detailed in Fig. 2, institute
It states base intracavity 1.12 and first communication port 1.13 is arranged on the base body.
The lid 1.2 includes lid ontology 1.21, lid inner cavity 1.22 and the second communication port 1.23, is detailed in Fig. 3, institute
It states lid inner cavity and second communication port is arranged on the lid ontology.
The clamping for clamping filter membrane is equipped on the inner wall of the base intracavity and on the inner wall of the lid inner cavity
Position, so that the inner cavity that the base intracavity and the lid inner cavity form is divided into and being set side by side and mutually independent by filter membrane
First accommodating chamber and the second accommodating chamber, the second communication port are connected to the second accommodating chamber, and the first communication port is connected to the first accommodating chamber,
Herein: the sample entered in the second accommodating chamber through the second communication port can partly or entirely enter described first through the filter membrane and hold
Chamber of receiving is filtered, and the filtrate in the first accommodating chamber is discharged from the first communication port.Preferably, the base intracavity 1.12 is circumferentially
Direction is equipped with the boss 1.14 towards the circular ring shape at center, and boss and base intracavity are formed together concave structure；The boss is used
In support and place filter membrane 2；The lower end of the lid ontology 1.21 be equipped with circular ring shape clamping platform 1.24, the clamping platform and
The fixation of filter membrane 2 is realized in the free end cooperation of the boss.In addition to this, further include on the boss be used to support place it is close
The position of seal (not shown) cooperates the fixing seal for realizing filter membrane by the clamping platform, the boss and sealing ring
(at least one is equipped with sealing ring between the preferably described clamping platform and filter membrane and between boss and filter membrane).
It is good that the upper end end of the lower end of the pedestal and the lid is equipped with burnishing surface and translucency
Observation window, observation window, the observation window on lid and filter membrane three on pedestal are arranged with central axis.The pedestal,
The lid and observation window are the good material of translucency, the material that the preferred light transmittance of the good material of translucency is 90% or more
Matter, such as polycarbonate.Herein preferably: the pedestal and observation window and the lid thereon and observation window thereon are one
Body structure.
The upper end of the base intracavity is equipped with internal screw thread A, and the lower end of the lid ontology is equipped with and the internal screw thread A phase
The external screw thread B matched realizes the detachable connection of pedestal and lid by the internal screw thread A on the pedestal and external screw thread B on lid,
Facilitate operation.
It is heretofore described filtering single-piece with a thickness of 10mm, H, does not include the height of the second communication port as shown in figure 1.
Using the technical solution of the present embodiment, specifically:
Filter membrane 2 is placed on the boss 1.14 of base intracavity 1.12；Sealing ring is placed on the mistake on boss 1.14
On filter membrane；The size and boss of sealing ring, which match, cannot be greater than boss；Pedestal and lid are passed through into internal screw thread and externally threaded
Cooperation is tightened until the lower end of clamping platform 1.24 is in contact with the filter membrane on boss.
Assembled filtering single-piece is placed under corresponding microscope；From 1.23 note of the second communication port in lid 1.2
Enter sample；Sample enters the second accommodating chamber, is filtered using filter membrane, and filtrate enters the first accommodating chamber；In first accommodating chamber
Filtrate be discharged from the first communication port 1.13.The whole process of sample filtering all carries out under microscopic conditions, can be real-time
The microcosmic real-time condition (whether there is or not whether complete retention, sample be for such as sample) when sample filtering is solved, convenient for being accurately controlled or adjusting
Whole filter condition is completed to filter and obtain (or analysis) sample with optimal conditions.
Fig. 4 is to be filtered using the filter device of the present embodiment to human lung adenocarcinoma cell HCC827, and in fluorescence microscopy
The entire filter process of shooting and monitoring, experimentation are as follows under mirror: human lung adenocarcinoma cell HCC827 is stably transfected GFP gene, can
Continually and steadily to express GFP (green fluorescent protein) as tracer label object.GFP can issue bright under the light excitation of 488nm
Green light.1 × 10 is added in 120 milliliters of physiological saline4A GFP-HCC827 cell, installs pipeline and peristaltic pump, filtering
It is 8 microns of trace-etching-film that aperture is placed in device, and entire filter device is placed in inverted fluorescence microscope, with 50 milliliters/
The flow velocity of minute is filtered；Switch white field light and the fluorescence visual field observes entire filter process under the microscope and shoots video
(being detailed in A-P process in Fig. 4).
As can be known from Fig. 4: A, G, K and P are the white field light visual field in Fig. 4, and B, C, D, E, F, H, I, J, L, M, N and O are in Fig. 4
The fluorescence visual field (scale in Fig. 4 in B and F is 50 microns).
In Fig. 4 during A-M: as time goes by, it can be observed that more and more GFP-HCC827 cells are trapped
On filter membrane, cellular morphology is complete and has green fluorescence.
In Fig. 4 during N-P: when filtering continues to 147 seconds, sample liquids have all been filtered, and air enters filter device,
Cell swells and ruptures.
It is absolutely proved by this experiment, the filter device of the present embodiment can effectively observe entire filter process, and right
The special event occurred in filter process is effectively monitored.
The foregoing is only a preferred embodiment of the present invention, is not intended to restrict the invention, for the skill of this field
For art personnel, the invention may be variously modified and varied.All within the spirits and principles of the present invention, made any to repair
Change, equivalent replacement, improvement etc., should all be included in the protection scope of the present invention.
Claims (10)
1. the filter device that one kind can use under the microscope, it is characterised in that: single-piece is filtered including at least one, it can be micro-
Filter process of the microscopic observation sample in filtering single-piece；
The filtering single-piece includes a shell with inner cavity, and inner cavity is separated into mutually independent at least two through filter membrane and accommodates
Chamber, the sample in an accommodating chamber can partly or entirely enter another accommodating chamber through filter membrane；The shell has at least one
A observation window, the filter membrane is it is observed that window is not less than one times of focal length of micro objective to the distance of microscopical object lens, just
Imaging is observed under the microscope in the sample on filter membrane.
2. the filter device according to claim 1 that can be used under the microscope, it is characterised in that: the filtering single-piece
Thickness is less than 20mm, preferably smaller than 15mm, more preferably less than 10mm.
3. the filter device according to claim 1 that can be used under the microscope, it is characterised in that: the filter membrane is through seeing
The minimum range for examining window to microscopical object lens is located at one times of focal length of micro objective between two focus length, the preferably described mistake
Filter membrane is it is observed that the minimum range of window to microscopical object lens is greater than one times of focal length of micro objective and is infinitely close to one times
Focal length.
4. the filter device according to claim 1 that can be used under the microscope, it is characterised in that: the filtering single-piece is more
A when being used in combination, the quantity at least two of the filtering single-piece, multiple filtering single-pieces are series connection between each other, arranged side by side
Or at least one of mutually indepedent setting.
5. the filter device according to any one of claims 1-4 that can be used under the microscope, it is characterised in that: described
Filtering single-piece includes lid and pedestal, the lid and the pedestal detachable connection；
The pedestal includes base body, base intracavity and the first communication port, the base intracavity and first communication port
It is arranged on the base body；The lid includes lid ontology, lid inner cavity and the second communication port, in the lid
Chamber and second communication port are arranged on the lid ontology；On the inner wall of the base intracavity and lid inner cavity
The fastening part for clamping filter membrane is equipped on inner wall, so that filter membrane is by the base intracavity and lid inner cavity group
At inner cavity be divided into mutually independent first accommodating chamber and the second accommodating chamber, first communication port and first accommodating chamber connect
Logical, the second communication port is connected to the second accommodating chamber；
The sample entered in the second accommodating chamber through the second communication port can partly or entirely enter described first through the filter membrane and hold
Chamber of receiving is filtered, and the filtrate in the first accommodating chamber is discharged from the first communication port；Either, enter first through the first communication port to hold
The sample received in chamber can be partly or entirely filtered into second accommodating chamber through the filter membrane, in the second accommodating chamber
Filtrate is discharged from the second communication port.
6. the filter device according to claim 5 that can be used under the microscope, it is characterised in that: the lower end of the pedestal
End and at least one observation window good equipped with translucency of the upper end end of the lid；It is preferred that on the pedestal and the lid
The good observation window of translucency is equipped on body, and the observation window on the observation window and the lid on the pedestal is corresponding sets
It sets, light can be injected from an observation window, after reaching and passing through the filter membrane, be projected from another observation window.
7. the filter device according to claim 6 that can be used under the microscope, it is characterised in that: the observation window is tool
There is the observation window of burnishing surface；
The material of the observation window is the good material of translucency or the pedestal, the lid and the observation window three
The material of person is the good material of translucency；
The good material of translucency refers to that light transmittance is the material of 50-100%, preferably glass, silica, polyvinyl chloride, polyphenyl
At least one of ethylene, polycarbonate, polymethyl methacrylate and methyl methacrylate.
8. the filter device according to claim 5 that can be used under the microscope, it is characterised in that: the base intracavity
Upper end is equipped with internal screw thread, and the lower end of the lid ontology is equipped with external screw thread matched with the internal thread；
Either, it is connected between the base body and the lid ontology by an at least trip.
9. the filter device according to claim 5 that can be used under the microscope, it is characterised in that: the base intracavity edge
Circumferencial direction is equipped with the boss towards the circular ring shape at center；The boss is for placing filter membrane；
The lower end of the lid ontology is equipped with the clamping platform of circular ring shape, and filter membrane is realized in the clamping platform and boss cooperation
Fixing seal.
10. the filter device according to claim 9 that can be used under the microscope, it is characterised in that: on the boss also
Including the position for sealing ring to be arranged, is cooperated by the clamping platform, the boss and sealing ring and realize consolidating for filter membrane
Fixed sealing.
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| CN201811172818.6A CN109107391B (en) | 2018-10-09 | 2018-10-09 | Filtering device capable of being used under microscope |
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| CN103937658A (en) * | 2014-03-28 | 2014-07-23 | 武汉介观生物科技有限责任公司 | Rare cell detection chip and application thereof |
| WO2015146682A1 (en) * | 2014-03-28 | 2015-10-01 | シャープ株式会社 | Filtration tool |
| CN103933780A (en) * | 2014-04-01 | 2014-07-23 | 西安交通大学 | Real-time dynamic observation system for fiber filtering |
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