CN109106874A - A method of extracting total polyphenols from maize peel - Google Patents

A method of extracting total polyphenols from maize peel Download PDF

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CN109106874A
CN109106874A CN201811170512.7A CN201811170512A CN109106874A CN 109106874 A CN109106874 A CN 109106874A CN 201811170512 A CN201811170512 A CN 201811170512A CN 109106874 A CN109106874 A CN 109106874A
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maize peel
room temperature
supernatant
total polyphenols
ultra high
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王新伟
赵仁勇
郭兴凤
李蕊
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Henan University of Technology
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Henan University of Technology
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    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/88—Liliopsida (monocotyledons)
    • A61K36/899—Poaceae or Gramineae (Grass family), e.g. bamboo, corn or sugar cane
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P3/00—Drugs for disorders of the metabolism
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P39/00—General protective or antinoxious agents
    • A61P39/06—Free radical scavengers or antioxidants
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/10—Preparation or pretreatment of starting material
    • A61K2236/15—Preparation or pretreatment of starting material involving mechanical treatment, e.g. chopping up, cutting or grinding
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/30—Extraction of the material
    • A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
    • A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/30—Extraction of the material
    • A61K2236/37—Extraction at elevated pressure or temperature, e.g. pressurized solvent extraction [PSE], supercritical carbon dioxide extraction or subcritical water extraction
    • A—HUMAN NECESSITIES
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    • A61K2236/50—Methods involving additional extraction steps
    • A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/50—Methods involving additional extraction steps
    • A61K2236/53—Liquid-solid separation, e.g. centrifugation, sedimentation or crystallization

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Abstract

A method of extracting total polyphenols from maize peel, the invention discloses the methods that total polyphenols are extracted from maize peel.The method includes pulverizing and sieving, degreasing, separating twice, solvent mixing, ultra high pressure treatment, centrifuge separation is concentrated by evaporation and freeze-drying, for maize peel functional component research and superhigh pressure technique using providing foundation.This method simple process, it is easy to operate, it is time saving and energy saving, new direction, new technology are provided for the development and utilization of corn deep processing byproduct maize peel, and enrich the source of plant polyphenol, there is preferable economic benefit and social benefit.

Description

A method of extracting total polyphenols from maize peel
Technical field
The method that the present invention relates to a kind of to extract total polyphenols from maize peel, belongs to polyphenol extractive technique field.
Background technique
China is maize production and consumption big country, and corn producing industry has occupied very big ratio in national economy.Corn Skin is the principal by product of corn deep processing, and accounts for about the 15% of corn kernel dry weight.Maize peel rich in nutrition content, except containing Fiber, starch, outside protein, also containing a variety of active ingredients such as polyphenol.It is removed freely some researches show that phenols substance has The effects of base, anti-oxidant, antiatherosclerosis, reducing blood pressure and blood fat.China's maize peel annual output is more than 2,000,000 t, but big portion Divide and do not carry out intensive processing, is only used for feed and fermentation industry, added value of product is low；Some are even directly discarded, both cause The significant wastage of resource also seriously pollutes environment.
Ultra high pressure treatment can destroy the structures such as the cell wall of material, cell membrane, promote cellular content and Extraction solvent It comes into full contact with, accelerates extraction rate, there is effective component caused by recovery rate is high, Extracting temperature is low and avoidable fuel factor The advantages that loss and physiological activity reduce.In recent years, ultra high pressure extraction technology be widely used to saponin(e, polysaccharide, oleanolic acid, In the extraction research of the bioactive ingredients such as essential oil of the flower of Camellia sinensis, and good effect is achieved, but related maize peel total polyphenols There is not been reported for the research of ultra high pressure extraction technology.Polyphenol is extracted from maize peel to be provided for the functional component research of maize peel Foundation, meanwhile, this method has simple process, time saving and energy saving feature.
Summary of the invention
The method that the object of the present invention is to provide a kind of to extract total polyphenols from maize peel.This method simple process, it is time-consuming It is short.The method includes pulverizing and sieving, degreasing, alcohol steep, ultra high pressure treatment, centrifuge separation, evaporation and concentration and freeze-drying.
The present invention includes the following steps:
1) it pulverizes and sieves: maize peel is crushed, cross 120 meshes and obtain maize peel powder.
2) degreasing: maize peel powder and ether are mixed according to the ratio (w/v) of 1:5, shaken at room temperature filters afterwards for 24 hours, filters Slag obtains defatted corn skin in 50 DEG C of low temperature dryings.
3) separating twice: defatted corn skin is crushed, and is crossed 120 meshes and is obtained defatted corn skin powder.
4) solvent mixes: by the defatted corn skin that crushed and 60% ethyl alcohol with solid-liquid ratio (1:30,1:40 or 1:50, It is preferred that 1:40) mixing, shaken at room temperature 1h.
5) ultra high pressure treatment: being placed in ultra high pressure treatment device after mixed liquor is vacuumized packaging, and adjusting temperature is room Temperature, pressure are 300-500(preferably 400) Mpa, dwell time 10-20(preferably 15) min.Then mixed liquor is depressurized and is filtered, received Collect filtrate.
6) it is centrifugated: smoke filtrate being put in centrifuge tube and is centrifuged, supernatant is taken.Filter residue is repeated again it is above-mentioned 4), 5) It is centrifuged to obtain supernatant after operation, supernatant is merged.
7) it is concentrated by evaporation: supernatant being placed in Rotary Evaporators and is concentrated.
8) it is freeze-dried: the liquid after concentration being taken out, is freeze-dried, maize peel polyphenol can be obtained.Polyphenol obtains Rate is 3.24-5.78mg/g.
The beneficial effects of the present invention are: inventor with 60% ethyl alcohol it is demonstrated experimentally that only extracted (except step 5) does not have to superelevation Pressure processing, other steps are same) in maize peel when total polyphenols, polyphenol yield is 1.23-2.21mg/g, use ultra high pressure treatment with Afterwards, polyphenol yield is 3.24-5.78mg/g, and the yield of maize peel total polyphenols substantially increases.The present invention is corn deep processing pair The development and utilization of product maize peel provide new direction, new technology, enrich the source of plant polyphenol, have preferable warp Benefit of helping and social benefit.
Specific embodiment
It is 4 examples that total polyphenols are extracted from maize peel below:
Embodiment 1:
1) it pulverizes and sieves: maize peel is crushed, cross 120 meshes and obtain maize peel powder.
2) degreasing: maize peel powder and ether are mixed according to the ratio (w/v) of 1:5, shaken at room temperature filters afterwards for 24 hours, filters Slag obtains defatted corn skin in 50 DEG C of low temperature dryings.
3) separating twice: defatted corn skin is crushed, and is crossed 120 meshes and is obtained defatted corn skin powder.
4) solvent mixes: the defatted corn skin that crushed being mixed with 60% ethyl alcohol with solid-liquid ratio 1:40, shaken at room temperature 1h.
5) ultra high pressure treatment: being placed in ultra high pressure treatment device after mixed liquor is vacuumized packaging, and adjusting temperature is room Temperature, pressure 300Mpa, dwell time 20min.Then mixed liquor is depressurized and is filtered, collect filtrate.
6) it is centrifugated: smoke filtrate being put in centrifuge tube and is centrifuged, supernatant is taken.Filter residue is repeated again it is above-mentioned 4), 5) It is centrifuged to obtain supernatant after operation, supernatant is merged.
7) it is concentrated by evaporation: supernatant being placed in Rotary Evaporators and is concentrated.
8) it is freeze-dried: the liquid after concentration being taken out, is freeze-dried, maize peel polyphenol can be obtained, polyphenol obtains Rate is 3.98mg/g.
Embodiment 2:
1) it pulverizes and sieves: maize peel is crushed, cross 120 meshes and obtain maize peel powder.
2) degreasing: maize peel powder and ether are mixed according to the ratio (w/v) of 1:5, shaken at room temperature filters afterwards for 24 hours, filters Slag obtains defatted corn skin in 50 DEG C of low temperature dryings.
3) separating twice: defatted corn skin is crushed, and is crossed 120 meshes and is obtained defatted corn skin powder.
4) solvent mixes: the defatted corn skin that crushed being mixed with 60% ethyl alcohol with solid-liquid ratio 1:50, shaken at room temperature 1h.
5) ultra high pressure treatment: being placed in ultra high pressure treatment device after mixed liquor is vacuumized packaging, and adjusting temperature is room Temperature, pressure 400Mpa, dwell time 10min.Then mixed liquor is depressurized and is filtered, collect filtrate.
6) it is centrifugated: smoke filtrate being put in centrifuge tube and is centrifuged, supernatant is taken.Filter residue is repeated again it is above-mentioned 4), 5) It is centrifuged to obtain supernatant after operation, supernatant is merged.
7) it is concentrated by evaporation: supernatant being placed in Rotary Evaporators and is concentrated.
8) it is freeze-dried: the liquid after concentration being taken out, is freeze-dried, maize peel polyphenol can be obtained, polyphenol obtains Rate is 4.57mg/g.
Embodiment 3:
1) it pulverizes and sieves: maize peel is crushed, cross 120 meshes and obtain maize peel powder.
2) degreasing: maize peel powder and ether are mixed according to the ratio (w/v) of 1:5, shaken at room temperature filters afterwards for 24 hours, filters Slag obtains defatted corn skin in 50 DEG C of low temperature dryings.
3) separating twice: defatted corn skin is crushed, and is crossed 120 meshes and is obtained defatted corn skin powder.
4) solvent mixes: the defatted corn skin that crushed being mixed with 60% ethyl alcohol with solid-liquid ratio 1:40, shaken at room temperature 1h.
5) ultra high pressure treatment: being placed in ultra high pressure treatment device after mixed liquor is vacuumized packaging, and adjusting temperature is room Temperature, pressure 400Mpa, dwell time 15min.Then mixed liquor is depressurized and is filtered, collect filtrate.
6) it is centrifugated: smoke filtrate being put in centrifuge tube and is centrifuged, supernatant is taken.Filter residue is repeated again it is above-mentioned 4), 5) It is centrifuged to obtain supernatant after operation, supernatant is merged.
7) it is concentrated by evaporation: supernatant being placed in Rotary Evaporators and is concentrated.
8) it is freeze-dried: the liquid after concentration being taken out, is freeze-dried, maize peel polyphenol can be obtained, polyphenol obtains Rate is 5.78mg/g.
Embodiment 4:
1) it pulverizes and sieves: maize peel is crushed, cross 120 meshes and obtain maize peel powder.
2) degreasing: maize peel powder and ether are mixed according to the ratio (w/v) of 1:5, shaken at room temperature filters afterwards for 24 hours, filters Slag obtains defatted corn skin in 50 DEG C of low temperature dryings.
3) separating twice: defatted corn skin is crushed, and is crossed 120 meshes and is obtained defatted corn skin powder.
4) solvent mixes: the defatted corn skin that crushed being mixed with 60% ethyl alcohol with solid-liquid ratio 1:40, shaken at room temperature 1h.
5) ultra high pressure treatment: being placed in ultra high pressure treatment device after mixed liquor is vacuumized packaging, and adjusting temperature is room Temperature, pressure 500Mpa, dwell time 10min.Then mixed liquor is depressurized and is filtered, collect filtrate.
6) it is centrifugated: smoke filtrate being put in centrifuge tube and is centrifuged, supernatant is taken.Filter residue is repeated again it is above-mentioned 4), 5) It is centrifuged to obtain supernatant after operation, supernatant is merged.
7) it is concentrated by evaporation: supernatant being placed in Rotary Evaporators and is concentrated.
8) it is freeze-dried: the liquid after concentration being taken out, is freeze-dried, maize peel polyphenol can be obtained, polyphenol obtains Rate is 3.51mg/g.

Claims (4)

1. a kind of method for extracting total polyphenols from maize peel, it is characterised in that include the following steps:
(1) it pulverizes and sieves: maize peel is crushed, cross 120 meshes and obtain maize peel powder；
(2) degreasing: maize peel powder and ether are mixed according to the ratio (w/v) of 1:5, and shaken at room temperature filters afterwards for 24 hours, filter residue in 50 DEG C of low temperature dryings, obtain defatted corn skin；
(3) separating twice: defatted corn skin is crushed, and is crossed 120 meshes and is obtained defatted corn skin powder；
(4) solvent mixes: by the defatted corn skin that crushed and 60% ethyl alcohol with solid-liquid ratio (1:30,1:40 or 1:50, preferably It 1:40) mixes, shaken at room temperature 1h；
(5) ultra high pressure treatment: being placed in ultra high pressure treatment device after mixed liquor is vacuumized packaging, and adjusting temperature is room temperature, Pressure is 300-500(preferably 400) Mpa, dwell time 10-20(preferably 15) min, then depressurizes mixed liquor and filters, and is collected Filtrate；
(6) be centrifugated: smoke filtrate is put in centrifuge tube and is centrifuged, supernatant is taken, filter residue is repeated again it is above-mentioned 4), 5) operate After be centrifuged to obtain supernatant, supernatant is merged；
(7) it is concentrated by evaporation: supernatant being placed in Rotary Evaporators and is concentrated；
(8) it is freeze-dried: the liquid after concentration is taken out to the as maize peel polyphenol being freeze-dried.
2. a kind of method for extracting total polyphenols from maize peel according to claim 1, it is characterised in that the described the 2) step, Maize peel powder and ether are mixed according to the ratio (w/v) of 1:5, shaken at room temperature filters afterwards for 24 hours, and filter residue is dried in 50 DEG C of low temperature It is dry, obtain defatted corn skin.
3. a kind of method for extracting total polyphenols from maize peel according to claim 1, it is characterised in that the 5) step will mix Close liquid vacuumize packaging after be placed in ultra high pressure treatment device, adjustings temperature be room temperature, pressure for 300-500(preferably 400) Then mixed liquor is depressurized and is filtered by Mpa, dwell time 10-20(preferably 15) min, collect filtrate.
4. a kind of method for extracting total polyphenols from maize peel according to claim 1, it is characterised in that the described the 8) step, Liquid taking-up after concentration is freeze-dried.
CN201811170512.7A 2018-10-09 2018-10-09 A method of extracting total polyphenols from maize peel Pending CN109106874A (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN116999516A (en) * 2023-07-06 2023-11-07 吉林农业大学 Green and efficient polyphenol extraction method from corn bran

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5361644A (en) * 1976-11-15 1978-06-02 Matsushita Electric Works Ltd Thermosetting resin molding materials
CN103204885A (en) * 2013-04-02 2013-07-17 吉林大学 Rapid extracting and separating method of corn filament polyphenol

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5361644A (en) * 1976-11-15 1978-06-02 Matsushita Electric Works Ltd Thermosetting resin molding materials
CN103204885A (en) * 2013-04-02 2013-07-17 吉林大学 Rapid extracting and separating method of corn filament polyphenol

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
谢昕: "玉米苞叶多酚提取工艺优化", 《农产品加工》 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN116999516A (en) * 2023-07-06 2023-11-07 吉林农业大学 Green and efficient polyphenol extraction method from corn bran
CN116999516B (en) * 2023-07-06 2024-05-17 吉林农业大学 Green and efficient polyphenol extraction method from corn bran

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