CN109106761A - Fork divides the application of knotweed, fork branch knotweed and manyspike knotweed herb total extract and its elution position in preparation treatment ulcerative colitis drug - Google Patents

Fork divides the application of knotweed, fork branch knotweed and manyspike knotweed herb total extract and its elution position in preparation treatment ulcerative colitis drug Download PDF

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CN109106761A
CN109106761A CN201811076623.1A CN201811076623A CN109106761A CN 109106761 A CN109106761 A CN 109106761A CN 201811076623 A CN201811076623 A CN 201811076623A CN 109106761 A CN109106761 A CN 109106761A
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knotweed
fork
manyspike
divides
herb
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董丽华
张忠立
朱玉野
左月明
罗光明
钟国跃
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Jiangxi University of Traditional Chinese Medicine
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Abstract

The present invention relates to disclose fork to divide the application of knotweed, fork branch knotweed and manyspike knotweed herb total extract and its elution position in preparation treatment ulcerative colitis drug.Prepared fork divides knotweed, fork branch knotweed and manyspike knotweed herb total extract itself to be that the ethanol water extraction for the 0-100% various concentration that fork divides knotweed or fork branch knotweed or manyspike knotweed herb is concentrated to give total extract, and above-mentioned fork divides knotweed or fork branch knotweed or manyspike knotweed herb total extract to be used to prepare treatment ulcerative colitis drug.Prepared fork divide knotweed, fork branch knotweed and manyspike knotweed herb elution position itself be fork divide knotweed or pitch the 0-100% various concentration of branch knotweed or manyspike knotweed herb ethanol water extract successively be eluted with water after macroporous absorbent resin on concentration total extract, 35% ethyl alcohol water elution, 70% ethyl alcohol water elution, 95% ethanol water afford water elution position, 35% ethyl alcohol water elution position, 70% ethyl alcohol water elution position, 95% ethyl alcohol water elution position, above-mentioned fork, which divides knotweed or pitches the elution position of branch knotweed or manyspike knotweed herb, to be used to prepare and treats ulcerative colitis drug.

Description

Fork divides knotweed, fork branch knotweed and manyspike knotweed herb total extract and its elution position to treat in preparation Application in ulcerative colitis drug
Technical field
The present invention relates to disclose fork knotweed, fork branch knotweed and manyspike knotweed herb total extract and its elution position is divided to treat in preparation Application in ulcerative colitis drug, belongs to technical field of traditional Chinese medicine preparation.
Background technique
Fork divides knotweed to be the drying that polygonaceae (Polygonaceae) Polygonum fork divides knotweed Polygonum divaricatum L. Root or all herbal medicine.The flavour of a drug are sour, bitter, puckery, cool in nature, dilute, light, puckery, blunt.There is the effect of heat-clearing, antidiarrheal, hemostasis, cure mainly intestines Shouting pain, just frequency is measured few, and just band purulence blood, miscellaneous to have mucus, the diseases such as tenesmus.
Manyspike knotweed herb is polygonaceae (Polygonaceae) Polygonum manyspike knotweed herb Polygonum polystachyum Wall.ex Meisn. the root or all herbal medicine of drying.With clearing heat and detoxicating, the effect of expelling wind and removing dampness.It is usually used in dysentery, diarrhea, children Indigestion, rheumatism swelling and pain, traumatic injury.
Pitching branch knotweed is the drying that polygonaceae (Polygonaceae) Polygonum pitches branch knotweed Polygonum tortuosum D.Don Root or all herbal medicine, be preferred wherein being used as medicine with the old root of black.Nature and flavor acid, sweet, warm.With dispelling cold, warm kidney cures mainly cold hernia, yin Capsule is perspired.
Fork divides knotweed, fork branch knotweed and manyspike knotweed herb to be common traditional herbal medicine, the diseases such as primary treatment enterogastritis, diarrhea, dysentery Disease, and it is resourceful, there is good development and utilization prospect, need further to be studied.The present invention confirm for the first time fork divide knotweed, Pitching branch knotweed and manyspike knotweed herb has the function curative effect for the treatment of ulcerative colitis (ulcerative colitis, UC).This experiment is adopted Ulcerative colitis (UC) rat model is established with 2,4,6- trinitrobenzene sulfonic acid (TNBS)/ethyl alcohol mixing method, research confirms fork Dividing knotweed or pitching branch knotweed or manyspike knotweed herb total extract and its elution position has obvious therapeutic action to UC rat model.To seek Clear curative effect, Small side effects are looked for, directly can mitigate or alleviate colitis reaction, improve therapeutic effect, reduce ulcerative colitis Scorching and other inflammatory bowel disease disease incidence improve quality of life of patients and all have a very important significance.The studies above does not have There is open report.
Summary of the invention
The invention discloses forks, and knotweed, fork branch knotweed and manyspike knotweed herb total extract and its elution position to be divided to treat ulcer in preparation Application in property colitis drug.
Foregoing invention uses following technical proposals.
Fork divides knotweed, fork branch knotweed and manyspike knotweed herb total extract and its preparation method for eluting position, it is characterised in that step is such as Under:
(1) fork divides knotweed or fork branch knotweed or manyspike knotweed herb pulverizing medicinal materials at coarse powder, is impregnated with 0-100% ethanol water, and 4- is added 12 times of amount solvent diacolations or heating and refluxing extraction several times, filter, merging filtrate.Above-mentioned filtrate decompression is concentrated to give water or difference Concentration ethanol total extract.
(2) the above-mentioned filtrate decompression of is concentrated into 0.5-1.8g raw medicinal herbs/ml, and refrigerated overnight analyses glue, filtering, and filtrate is used again Organic solvent extraction or the upper macroporous absorbent resin of filtrate carry out gradient elution, recycle organic solvent, obtain each elution position；
More specifically, be added 4-12 times in step (1) and measure solvent heating and refluxing extraction 2-4 times, each 1-3 hours.
More specifically, in step (2), in 0-4 DEG C of refrigerated overnight.
More specifically, it is 45-80 DEG C that temperature, which is concentrated under reduced pressure, in step (2).
More specifically, in step (2), filtrate through macroporous absorbent resin (AB-8, D101, HPD100, HPD300 and The macroreticular resin of the various known models such as HPD600) enrichment, it is successively eluted with water, the washing of 35% ethyl alcohol water elution, 70% ethyl alcohol De-, 95% ethyl alcohol water elution collects water elution position, 35% ethyl alcohol water elution position, 70% ethyl alcohol water elution position, 95% Ethyl alcohol water elution.
The fork of preceding method preparation divides knotweed or pitches branch knotweed or manyspike knotweed herb total extract and its elution position and can be used for preparation and control Ulcerative colitis drug is treated, the preparation formulation of the drug includes oral solution, granule, capsule, tablet, effervescent tablet, sugar Starch agent, pill, paste nourishing agent, soft capsule, ointment, emulsion, powder, sustained release agent, controlled release agent, targeting preparation, powder-injection, water needle Agent, injection, Alevaire, microemulsion, gelling agent, nanometer formulation or various known formulations dosage forms or various acceptable preparation agent Type, the drug further include various folk prescriptions and compound preparation, and said medicine and its preparation have treatment ulcerative colitis disease Disease.
The invention has the advantages that providing fork divides knotweed, fork branch knotweed and manyspike knotweed herb total extract and its extraction for eluting position With preparation method, gained total extract and its elution position can be used for preparing treatment ulcerative colitis drug oral solution, Granula, capsule, tablet, effervescent tablet, syrup, pill, paste nourishing agent, soft capsule, ointment, emulsion, powder, sustained release agent, control Release agent, targeting preparation, powder-injection, liquid drugs injection, injection, Alevaire, microemulsion, gelling agent, nanometer formulation or various known agent The bulk pharmaceutical chemicals of the drug of type or various acceptable preparation formulations.
Specific embodiment
The present invention will be further described below with reference to examples:
Embodiment 1
Fork divides the preparation method of knotweed total extract, in which: and divide knotweed pulverizing medicinal materials at coarse powder fork, is soaked in water 1 hour, 8 The heating of amount water, which decocts, again extracts 3 times, 2 hours every time, filters, merging filtrate.Above-mentioned filtrate decompression is concentrated to give water total extract.
Embodiment 2
Pitch the preparation method of branch knotweed total extract, in which: fork branch knotweed pulverizing medicinal materials are soaked in water 1 hour, 8 times at coarse powder It measures water heating and decocts extraction 3 times, 2 hours every time, filter, merging filtrate.Above-mentioned filtrate decompression is concentrated to give water total extract.
Embodiment 3
The preparation method of manyspike knotweed herb total extract, in which: by manyspike knotweed herb pulverizing medicinal materials at coarse powder, it is soaked in water 1 hour, 8 The heating of amount water, which decocts, again extracts 3 times, 2 hours every time, filters, merging filtrate.Above-mentioned filtrate decompression is concentrated to give water total extract.
Embodiment 4
Fork divides knotweed ethanol total extract and its elutes the preparation method at position, in which: divides knotweed pulverizing medicinal materials at thick fork Powder, with 75% ethyl alcohol impregnate 1 hour, 8 times amount solvent heating and refluxing extraction 3 times, 2 hours every time, filter, merging filtrate.It is above-mentioned Filtrate decompression is concentrated to give ethanol total extract.Or above-mentioned filtrate is concentrated under reduced pressure into 1.0g raw medicinal herbs/ml at 65 DEG C, 0-4 DEG C is cold Hiding is stayed overnight, analysis glue, centrifugal filtration, and AB-8 model macroporous adsorbing resin for purification on filtrate is successively eluted with water, 35% ethanol water Elution, 70% ethyl alcohol water elution and 95% ethyl alcohol water elution, obtain water elution position, 35% ethyl alcohol water elution position, 70% second Alcohol water elution position and 95% ethyl alcohol water elution position.
Embodiment 5
It pitches branch knotweed ethanol total extract and its elutes the preparation method at position, in which: by fork branch knotweed pulverizing medicinal materials at thick Powder, with 75% ethyl alcohol impregnate 1 hour, 8 times amount solvent heating and refluxing extraction 3 times, 2 hours every time, filter, merging filtrate.It is above-mentioned Filtrate is concentrated under reduced pressure into 1.0g raw medicinal herbs/ml at 65 DEG C, 0-4 DEG C of refrigerated overnight, analyses glue, centrifugal filtration, AB-8 model on filtrate Macroporous adsorbing resin for purification is successively eluted with water, 35% ethyl alcohol water elution, 70% ethyl alcohol water elution and 95% ethyl alcohol water elution, Obtain water elution position, 35% ethyl alcohol water elution position, 70% ethyl alcohol water elution position and 95% ethyl alcohol water elution position.
Embodiment 6
Manyspike knotweed herb ethanol total extract and its preparation method for eluting position, in which: by manyspike knotweed herb pulverizing medicinal materials at thick Powder, with 75% ethyl alcohol impregnate 1 hour, 8 times amount solvent heating and refluxing extraction 3 times, 2 hours every time, filter, merging filtrate.It is above-mentioned Filtrate is concentrated under reduced pressure into 1.0g raw medicinal herbs/ml at 65 DEG C, 0-4 DEG C of refrigerated overnight, analyses glue, centrifugal filtration, AB-8 model on filtrate Macroporous adsorbing resin for purification is successively eluted with water, 35% ethyl alcohol water elution, 70% ethyl alcohol water elution and 95% ethyl alcohol water elution, Obtain water elution position, 35% ethyl alcohol water elution position, 70% ethyl alcohol water elution position and 95% ethyl alcohol water elution position.
Embodiment 7
The present invention establishes ulcerative colitis (UC) greatly using 2,4,6- trinitrobenzene sulfonic acid (TNBS)/ethyl alcohol mixing method Mouse model, research fork divide therapeutic effect of the knotweed total extract to UC rat model, it is intended to divide the clinical practice of knotweed medicinal material to answer for fork With providing scientific basis, while laying the foundation for the research of its effective substance and mechanism of action.
1 experimental material
1.1 drugs take fork to divide knotweed dry herb 1.0kg, are ground into coarse powder, and 75% ethyl alcohol impregnates, and refluxing extraction 3 times, often It secondary 2 hours, filters, merging filtrate, concentration and recovery ethyl alcohol, vacuum distillation is concentrated and dried to obtain ethanol total extract.Weigh experiment institute Ethanol total extract 54.1g is needed, divides knotweed ethanol total extract with 0.5% carboxymethyl cellulose (CMC- Na) solution preparation fork (8g/kg, 4g/kg, 2g/kg) dosage group, saves backup.Sulfasalazine tablet (SASP) (the limited public affairs of Shanghai Xinyi balance medicine company Department, lot number: 09170519)
1.2 SPF grades of animal SD male rats 60,6-8 week old, weight 180-220g are reached purchased from Hunan Si Laike scape Experimental animal Co., Ltd, credit number are SCXK (Hunan) 2016-0002.Rearing conditions: laboratory temperature is controlled in 20-25 DEG C, humidity 50%-60% is fed with Rat Standard feed, free water.
1.3 reagent 2,4,6- trinitrobenzene sulfonic acid (TNBS) (are purchased from from Sigma Co., USA and dispense)；Medullary substance peroxidating Enzyme reagent kit (MPO), superoxide dismutase kit (SOD), Malondialdehyde Kit (MDA), nitric oxide kit (NO) It is purchased from Nanjing and builds up Bioengineering Research Institute；IL-1 β and TNF-α kit, which are purchased from, to be purchased from the prosperous biotechnology in Nanchang and has Limit company.
1.4 instrument UV-1800 ultraviolet-uisible spectrophotometers (Japanese Shimadzu Corporation)；JW-3021HR high speed freeze from Scheming (Anhui Jia Wen instrument and equipment Co., Ltd)；MULTISKAN GO microplate reader (Thermo Fischer Scient Inc.).
2 experimental methods
SD rat is randomly divided into normal group, model group, SASP group by the foundation of 2.1 animal packets and UC model (400mg/kg), fork divide knotweed ethanol total extract basic, normal, high (2g/kg, 4g/kg, 8g/kg) dosage group, and every group 10.It adapts to After raising 5 days, UC model is established.UC model is prepared using 2,4,6- trinitrobenzene sulfonic acid (TNBS)/ethyl alcohol mixing bowel lavage.It makes After being deprived of food but not water 24 hours before mould, 2.5ml/kg dosage intraperitoneal injection of anesthesia is pressed with 10% chloraldurate, with rat oral gavage needle At insertion anus 8cm gently, 50mg/kgTNBS+0.25ml50% alcohol mixeding liquid is slowly injected into rat colon, then Rat is inverted 1 minute or so, is put in mouse cage, and body is made to lie on the back placements, after regaining consciousness naturally, free diet.Normal group Rat is only injected into isometric 0.9% normal saline solution bowel lavage.
2.2 administrations modeling the 2nd day, it is filthy that loose stools, hematochezia and crissum occurs in rat, by above-mentioned dosage gastric infusion, Normal group and model group give same amount of normal saline.1 time a day, successive administration 7 days.
After 2.4 collect specimens last time is administered, fasting 12h, first with 10% chloral hydrate anesthesia, abdominal aorta is taken Blood, serum is saved in -20 DEG C of refrigerator freezings after centrifugation, and the biochemical indicator for serum detects.Then rat neck is taken off to put to death, It takes away from anus 7-13cm lesion colon, removes excrement, while carrying out the observation of colonic tissue general form, and take pictures, use Scissors is longitudinally cut off along colon, with 0.9% cold saline washes clean.Colon is finally cut into two sections, a part is put into jelly Deposit in pipe, be immediately placed in liquid nitrogen, after be put into -70 DEG C of refrigerators and save, the measurement for MDA, MOP, SOD；A part is placed in It is fixed in 10% formalin solution, for routine pathology slice and HE dyeing.
3 results
The observation of 3.1UC rat ordinary circumstance is scored with disease activity index (DAI)
Normal rats hair color living is pure white smooth, lively and sensitive, and stool is normal, body weight increase, no diarrhea, hematochezia and dead Die situation.Moved less by occurring as soon as within the 2nd day after TNBS/ ethyl alcohol modeling rat, like flocking together, binocular dimness it is straight without mind, hair Vertical, anorexia, weight loss stool is shapeless, and loose stools, crissum filth, individual visually visible mucus bloody stools occurs in serious person.Administration Above-mentioned symptom is improved in varying degrees afterwards, and wherein SASP group and fork divide the middle and high dosage group rat of knotweed alcohol extract in weight, appetite It is significantly improved with bloody stool situation.Each group UC rat disease activity index (DAI) as shown in table 1, compared with normal group, Model group shows significant difference (p < 0.01).Compared with model group, SASP group, fork divides the performance of knotweed alcohol extract high dose group extremely aobvious It writes difference (p < 0.01), fork divides knotweed alcohol extract middle dose group to show significant difference (p < 0.05), and fork divides knotweed alcohol extract low dose group It shows not statistically significant.
Table 1UC rat disease activity index (DAI) scoring (N=8)
Note: compared with normal group, P < 0.01 * P < 0.05, * *；Compared with model group▲P < 0.05,ΔΔP<0.01
3.2UC Colonic Mucosa of The Rat damages general form observation
Normal rats colon color is fresh, oedema, hyperemia and ulcerative phenomena does not occur, and colon surface is smooth, has bullet Property, duplicature is clear.Model group rats colon darker in color, intestinal wall thickens, stenosis of bowel, is adhered with surrounding tissue, intestinal mucosa Serious congestion and edema, erosion form ulcer, and colon lengths shorten.After administration, SASP group, fork divide knotweed alcohol extract high Dosage group rat colon color is improved, and substantially without hyperemia, extravasated blood, oedema, rotten to the corn and ulcer area obviously subtracts mucous membrane of colon It is few.Fork divides knotweed alcohol extract middle dose group colon color also to be improved, intestinal mucosa mild hyperaemia, oedema, exist it is certain rotten to the corn and Ulcer area, but with the obvious mitigation of model group.And it pitches and knotweed alcohol extract low dose group above situation is divided not changed substantially It is kind.
3.3UC Colonic Mucosa of The Rat pathological study and scoring
It observes under an optical microscope, normal Colon mucosa cell of organizing is without deformation, structural integrity, no necrosis, inherently Layer and lower layer, muscle layer are without cell infiltration.Model group colonic mucosa falls off, and goblet cell disappears, and is seriously soaked by inflammatory cell Profit, granulation tissue and fibroblast proliferation, and generate crypt abscess.Model group rats colon mucosa tissues histological scores are aobvious It writes and is higher than normal group.After administration, SASP group, fork divide knotweed alcohol extract high dose group histological scores compared with the extremely significant decline of model group, Fork divides knotweed alcohol extract middle dose group performance significant difference compared with model group；Fork divides knotweed alcohol extract low dose group above-mentioned symptom Have no improvement, the no difference of science of statistics compared with model group.Grade form is shown in Table 2.
2 UC rat colon score of tissue damage of table (N=8)
Note: compared with normal group, P < 0.01 * P < 0.05, * *；Compared with model group▲P < 0.05,ΔΔP<0.01
3.4 forks divide influence of the knotweed extract to MPO, MDA and SOD in UC rat colon tissue
Compared with normal group, MPO, MDA content significantly increase (P < 0.01) in model group rats colonic tissue, SOD vigor It is decreased obviously (P < 0.01).Compared with model group, SASP group and fork divide that knotweed alcohol extract is high, MDA contains in middle dose group rat colon Measure extremely significant reduction (P < 0.01)；SASP group and fork divide the extremely significant reduction of MPO content in knotweed alcohol extract high dose group rat colon (P < 0.01), MPO content significantly reduces (P < 0.05) in middle dose group rat colon；SASP group and fork divide knotweed alcohol extract high agent The extremely significant raising (P < 0.01) of SOD content in amount group rat colon, SOD content significantly increases (P in middle dose group rat colon <0.05).And it pitches and knotweed alcohol extract low dose group MPO, MDA and SOD is divided to make moderate progress compared with model group, but is poor without statistics It is different.It the results are shown in Table 3
Table 3 pitch divide knotweed extract to MPO, MDA and SOD in UC rat colon tissue influence (N=8)
Note: compared with normal group, P < 0.01 * P < 0.05, * *；Compared with model group▲P < 0.05,ΔΔP<0.01
3.5 forks divide influence of the knotweed alcohol extract to NO, TN F- α and IL-1 β changes of contents in UC rat blood serum
Compared with normal group, model group rats serum NO, TNF-α and IL-1 β content are significantly raised (P < 0.01).With Model group compares, SASP group, fork divide knotweed alcohol extract high dose group serum NO, TNF- α and IL-1 β content be decreased obviously (P < 0.01), three's content is remarkably decreased (P < 0.05) in middle dose group serum；Three's content is although also have in low dose group serum Decline, but the no difference of science of statistics compared with model group.
Table 4 pitch divide knotweed to NO, TNF-, IL-1 β and IL-10 content in UC rat blood serum influence (N=8)
Note: compared with normal group, P < 0.01 * P < 0.05, * *；Compared with model group▲P < 0.05,ΔΔP<0.01
Embodiment 8
The present invention establishes ulcerative colitis (UC) greatly using 2,4,6- trinitrobenzene sulfonic acid (TNBS)/ethyl alcohol mixing method Mouse model, research fork divide water elution position, 35% ethyl alcohol water elution position, 70% ethyl alcohol water elution position and 95% second of knotweed Therapeutic effect of the alcohol water elution position to UC rat model, it is intended to which the research for its effective substance and mechanism of action is established Basis.
The preparation of 1.1 drugs: fork knotweed herb being crushed, is extracted using percolation, extracting solution is carried out to be concentrated into paste, Recycle ethyl alcohol.It will be so concentrated extract carries out large pore resin absorption column chromatographic isolation, successively with water, 35% ethyl alcohol, 70% second Alcohol, 95% ethyl alcohol difference gradient elution are concentrated to get water elution position, 35% ethyl alcohol water elution position, 70% ethanol water respectively Elute position, 95% ethyl alcohol water elution position.
1.2 animal packets: cleaning grade male SD rat 60, weight (180~220g), 6-8 week old.By SD rat Using being randomly divided into 11 groups, every group 10, it is respectively as follows: Normal group, model group, positive drug sulfasalazine (SASP) group (400mg/kg), water elution position high dose group (760mg/kg), water elution position low dose group (190mg/kg), 35% second Alcohol water elution position high dose group (303mg/kg), 35% ethyl alcohol water elution position low dose group (76mg/kg), 70% ethyl alcohol Water elution position high dose group (74mg/kg), 70% ethyl alcohol water elution position low dose group (18mg/kg), 95% ethanol water Elute position high dose group (20mg/kg), 95% ethyl alcohol water elution position low dose group (5mg/kg).
1.3 experimental results:
1.3.1 general state is observed: daily observation rat spirit, fur, stool, activity, diet, death condition claim body Quality simultaneously records.Referring to Cooper HS points-scoring system, disease activity index (DAI) scoring is carried out.Normal rats react ratio Sensitiveer, hair is glossy, and diet is normal, body weight increase, no diarrhea, hematochezia and death condition.Model group then shows as spirit Show that dispirited, few dynamic, hair is upright, weight loss, loose stools etc..Compared with model group, positive drug, 35% ethyl alcohol water elution portion The high low dose group difference compared with model group in position is extremely significant (p < 0.01)；70% ethyl alcohol water elution position, water position high dose group It is significant (p < 0.05) with model group comparing difference；Each dosage group in other positions compared with model group although declined, nothing Statistical difference.
13.2UC Colonic Mucosa tissue damage gross examination of skeletal muscle: visually observe Colonic Mucosa tissue: normal group is big Mouse colon uniform elongate, surface is smooth, duplicature complete display；And model group rats colon occur intestinal wall thicken, enteric cavity it is narrow It is narrow, be adhered with surrounding tissue, lesion colonic mucosa congestion and edema, erosion, formed the big ulcer of superficial.SASP group rat colon with Model group relatively has no that obvious congested, extravasated blood, diseased region are improved, hence it is evident that mitigates.Water position, 35% ethyl alcohol water elution Position, the high low dose group rat colon hyperemia in 75% ethyl alcohol water elution position, extravasated blood, intestines color and muscle layer thickness and model group Mutually there is different degrees of mitigation, wherein, 95% ethyl alcohol water elution position and model group the most significant with 35% ethyl alcohol water elution position Compare no the change of divergence.
1.3.3 influence of the different elution positions to TNF-α, IL-1 β level in UC rat blood serum: in UC rat blood serum TNF-α, IL-1 β changes of contents, compared with Normal group, TNF- α, IL-1 β content are significantly increased in model group rats serum (P<0.01)；Compared with model group, TNF-α, IL- in positive drug SASP group, 35% ethyl alcohol water elution position high dose group serum 1 β content is remarkably decreased, and is showed extremely significant difference (P < 0.01), TNF-α in 35% ethyl alcohol water elution position low dose group serum, The decline of IL-1 β content, shows extremely significant difference (P < 0.05)；Water position high dose group, 75% ethyl alcohol water elution position high dose It is significant (P < 0.05) with model group comparing difference, although remaining administration group is declined slightly compared with model group, no statistics Difference.
1.3.4 influence of the different elution positions to UC rat colon tissue MPO: compared with normal group, model group rats knot MPO content is apparently higher than normal group (P < 0.01) in intestinal tissue, and compared with model group, positive drug group, the high low dosage in each position are given Medicine group MPO content has different degrees of decline, and wherein SASP group, the performance of 35% ethyl alcohol water elution position high dose group are extremely significant Difference (P < 0.01), 35% ethyl alcohol water elution position low dose group show extremely significant difference (P < 0.05), water position high dose Group, 75% ethyl alcohol water elution position high dose group performance significant difference (P < 0.05), remaining regional administration group is compared with model group There was no significant difference.
1.3.5 influence of the different elution positions to the mRNA expression of TLR4 in UC rat colon tissue: with normal group ratio Compared with the expression of the mRNA of TLR4 is apparently higher than normal group (P < 0.01) in model group rats colonic tissue, each position various dose Height is more normally organized in the expression of the mRNA of group TLR4；Compared with model group, SASP group, the 35% high low dosage in ethyl alcohol water elution position The expression of the mRNA of group TLR4 is extremely significant to reduce (P < 0.01), water position, 70% ethyl alcohol water elution position high dose TLR4 The expression of mRNA significantly reduces (P < 0.05), the expression of the mRNA of 95% ethyl alcohol water elution position TLR4 nothing compared with model group Statistical difference.
1.3.6 influence of the different elution positions to the mRNA expression of NF- κ Bp65 in UC rat colon tissue: with normal group Compare, the mRNA expression of NF- κ Bp65 is apparently higher than normal group (P < 0.01) in model group rats colonic tissue, and each position is different The relatively normal group of the mRNA expression of dosage group NF- κ Bp65 is high；Compared with model group, SASP group, 35% ethyl alcohol water elution position height The extremely significant reduction (P < 0.01) of low dose group, water position, 70% ethyl alcohol water elution position high dose significantly reduce (P < 0.05), 95% ethyl alcohol water elution position no difference of science of statistics compared with model group.

Claims (4)

1. fork divides the application of knotweed, fork branch knotweed and manyspike knotweed herb total extract in preparation treatment ulcerative colitis drug, feature Be: fork divides knotweed, fork branch knotweed and manyspike knotweed herb total extract itself to be that fork divides the 0-100% of knotweed or fork branch knotweed or manyspike knotweed herb difference dense The ethanol water extraction of degree is concentrated to give total extract, and it is routed that above-mentioned fork divides knotweed or fork branch knotweed or manyspike knotweed herb total extract to be used to prepare treatment Ulcer colitis drug.
2. fork divides application of the elution position of knotweed, fork branch knotweed and manyspike knotweed herb in preparation treatment ulcerative colitis drug, special Sign is: fork divides the elution position itself of knotweed, fork branch knotweed and manyspike knotweed herb to be that fork divides the 0-100% of knotweed or fork branch knotweed or manyspike knotweed herb not Be successively eluted with water after macroporous absorbent resin on the ethanol water total extract of concentration, 35% ethanol elution, 70% ethanol elution, 95% ethanol elution obtains water elution position, 35% alcohol elution, 70% alcohol elution, 95% ethanol elution portion Position, above-mentioned fork divide the elution position of knotweed or fork branch knotweed or manyspike knotweed herb to be used to prepare treatment ulcerative colitis drug.
3. according to claim 1, fork described in any one of 2 divides knotweed, the total extract of fork branch knotweed and manyspike knotweed herb and its elution position Application in preparation treatment ulcerative colitis drug, it is characterised in that: the preparation formulation of the drug is oral solution, particle Agent, capsule, tablet, effervescent tablet, syrup, pill, paste nourishing agent, soft capsule, ointment, emulsion, powder, sustained release agent, controlled release Agent, targeting preparation, powder-injection, liquid drugs injection, injection, Alevaire, microemulsion, gelling agent, nanometer formulation.
4. according to claim 1, fork described in any one of 2 divides knotweed, the total extract of fork branch knotweed and manyspike knotweed herb and its elution position Application in preparation treatment ulcerative colitis drug, it is characterised in that: the drug further includes various folk prescriptions and compound system Agent.
CN201811076623.1A 2018-09-14 2018-09-14 Fork divides the application of knotweed, fork branch knotweed and manyspike knotweed herb total extract and its elution position in preparation treatment ulcerative colitis drug Pending CN109106761A (en)

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