CN109100189B - Cell tissue sample film-making device - Google Patents
Cell tissue sample film-making device Download PDFInfo
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- CN109100189B CN109100189B CN201810749044.2A CN201810749044A CN109100189B CN 109100189 B CN109100189 B CN 109100189B CN 201810749044 A CN201810749044 A CN 201810749044A CN 109100189 B CN109100189 B CN 109100189B
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- 230000002380 cytological effect Effects 0.000 claims description 14
- 244000261422 Lysimachia clethroides Species 0.000 claims description 4
- 239000005357 flat glass Substances 0.000 claims description 3
- 230000000149 penetrating effect Effects 0.000 claims description 3
- 239000011521 glass Substances 0.000 abstract description 25
- 239000006059 cover glass Substances 0.000 abstract description 11
- 230000033001 locomotion Effects 0.000 abstract description 9
- 238000000034 method Methods 0.000 description 5
- 230000000694 effects Effects 0.000 description 4
- 230000007246 mechanism Effects 0.000 description 4
- 230000008569 process Effects 0.000 description 4
- 238000002474 experimental method Methods 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 230000008859 change Effects 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000002745 absorbent Effects 0.000 description 1
- 239000002250 absorbent Substances 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 238000010009 beating Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000003111 delayed effect Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000007599 discharging Methods 0.000 description 1
- 238000006073 displacement reaction Methods 0.000 description 1
- 239000000975 dye Substances 0.000 description 1
- 230000008092 positive effect Effects 0.000 description 1
- 230000007704 transition Effects 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
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Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/286—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q involving mechanical work, e.g. chopping, disintegrating, compacting, homogenising
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/34—Microscope slides, e.g. mounting specimens on microscope slides
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- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Optics & Photonics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Sampling And Sample Adjustment (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The invention provides a cell tissue sample slide making device which comprises an object carrying seat, an object carrying table, a slide making seat and a slide making table, wherein a first strip-shaped hole and a right-angle fixing plate are arranged on the object carrying table, two first movable rods, a screw rod and a first motor are symmetrically arranged below the object carrying table, an Archimedes spiral groove is arranged on the slide making table, a second movable rod is arranged in the Archimedes spiral groove, and the second movable rod is sequentially connected with a second motor, a hollow cylindrical cam third movable rod and a hammer head. The invention utilizes the first motor to drive the right-angle fixed plate to clamp the glass slide and the cover glass, utilizes the second motor to drive the third movable rod to do reciprocating linear motion to beat and move bubbles, and the spotlight enables an operator to more carefully observe the bubbles remained in the sample.
Description
Technical Field
The invention belongs to the technical field of middle school student experimental equipment, and particularly relates to a cell tissue sample slide making device.
Background
The middle school biology requires students to learn how to use a microscope and to master basic methods for preparing specimens, and particularly, when students just come into contact with biology, the students need to know some cell structures by preparing specimens. The quality of the tabletting is related to the observation and detection effects.
At present, after a plurality of cell tissue samples are placed on a glass slide, a specific solution is needed to be applied, then the glass slide is covered on the top of the glass slide, after the glass slide is covered on the glass slide, water between the glass slide and the glass slide needs to be absorbed by absorbent paper, meanwhile, a certain number of bubbles are generated between the glass slide and the glass slide, a slide producer needs to continuously beat the glass slide by a small hammer or a hard device is used for gradually discharging the bubbles around the cell samples along the upper surface of the glass slide, so that the speed of searching target cells in the microscopic examination process is increased, and the observation effect of the displacement interference of the bubble accumulation on a dyeing agent is avoided; the stability is poor when the cover glass and the glass slide are combined manually, more bubbles are generated, and the quality of the slide is influenced to a certain extent; moreover, the work load of exhausting bubbles in the process of preparing a certain number of samples is quite large, the time and the laboratory for middle school students to do experiments are very limited, the teacher teaching progress can be greatly delayed if the efficiency of preparing the film cannot be improved, and the enthusiasm of the students can be gradually reduced.
Disclosure of Invention
Aiming at the technical problem of eliminating bubbles between a glass slide and a cover glass in the sample slide making process, the invention provides the cell tissue sample slide making device which is reasonable in design, simple in structure, convenient to use and high in slide making efficiency.
In order to achieve the above object, the invention adopts the technical scheme that the cytological tissue sample slide making device comprises a carrying seat and a slide making seat, wherein the carrying seat is provided with a supporting table, the supporting table is provided with a carrying table, the upper surface of the carrying table is provided with four first strip-shaped holes and four right-angle fixing plates, the lower surface of the carrying table is symmetrically provided with two first movable rods, the first movable rods are provided with two second strip-shaped holes, the right-angle fixing plates are provided with bolts penetrating through the first strip-shaped holes and the second strip-shaped holes, two sides of the supporting table are provided with bearing seats, the bearing seats are connected with a lead screw, the lead screw penetrates through the first movable rods and is connected with a first motor, the carrying seat is arranged on the slide making seat, the slide making seat is provided with a slide making table, and the slide making table is provided with an archimedes spiral groove, the hydraulic hammer is characterized in that a second movable rod is arranged in the Archimedes spiral groove, a second motor is arranged at the bottom of the second movable rod, a hollow cylindrical cam is arranged at the output end of the second motor, a third movable rod is arranged inside the hollow cylindrical cam, and a hammer head is arranged at the bottom of the third movable rod.
Preferably, the second motor is provided with a connecting cylinder, the hollow cylindrical cam is arranged in the connecting cylinder, the outer wall of the connecting cylinder is provided with a movable ring, the movable ring is connected with a C-shaped frame, and the C-shaped frame is fixedly connected with the third movable rod.
Preferably, a sliding guide head is arranged at the top of the third movable rod, and a track groove for the sliding guide head to move is formed in the inner wall of the hollow cylindrical cam.
Preferably, a light-gathering hole is formed in the center of the stage, flat glass is arranged in the light-gathering hole, and a spotlight corresponding to the light-gathering hole is arranged in the support table.
Preferably, the first strip-shaped holes are radially distributed along the center of the light gathering hole.
Preferably, the first motor is arranged in a first motor box, and the first motor box is arranged on one side of the object holder.
Preferably, the side surface of the right-angle fixing plate is provided with an exhaust hole, and the inner wall of the side surface of the right-angle fixing plate is provided with a right-angle groove.
Preferably, the slide making seat is a gooseneck type seat, and a carrying groove for placing the carrying seat is arranged on the slide making seat.
Preferably, a handle is arranged at the top of the second movable rod.
Compared with the prior art, the invention has the advantages and positive effects that,
1. according to the cell tissue sample slide-making device provided by the invention, the first movable rod is moved by utilizing the rotation of the screw rod and drives the two right-angle fixing plates on the same side to be close to or far away from the center of the object stage along the first strip-shaped hole, so that a glass slide and a cover glass on the object stage are clamped or loosened, and meanwhile, the sample on the glass slide can be illuminated by opening a spotlight, so that an operator can observe bubbles remained in the sample more carefully; the second motor is utilized to drive the cylindrical cam to rotate so that the third movable rod makes reciprocating linear motion, and therefore the purpose of knocking bubbles and moving the bubbles is achieved; the second motor, the cylindrical cam and the third movable rod can rotate along the Archimedes spiral groove by rotating the handle, so that air bubbles are gradually knocked and pushed out of the edges of the glass slide and the cover glass. The device completes the work of removing the bubbles through machinery, reduces the manual workload, greatly reduces the number of the bubbles in the obtained sample, saves the time of student experiments, has excellent observation effect, and is reasonable in design, simple in structure and suitable for large-scale popularization.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings needed to be used in the description of the embodiments are briefly introduced below, and it is obvious that the drawings in the following description are some embodiments of the present invention, and it is obvious for those skilled in the art to obtain other drawings based on these drawings without inventive labor.
FIG. 1 is a sectional view of a device for preparing a cytological tissue specimen, according to an exemplary embodiment;
FIG. 2 is a side view of a device for preparing a cytological tissue specimen, according to an exemplary embodiment;
FIG. 3 is a top view of a device for preparing a cytological tissue specimen, according to an exemplary embodiment;
FIG. 4 is a bottom view of the stage according to an exemplary embodiment;
FIG. 5 is an isometric view of the overall mechanism of a cytological tissue specimen preparation apparatus according to an embodiment;
in the above figures, 1, a carrier base; 2. a support table; 3. an object stage; 31. a first bar-shaped hole; 4. a right-angle fixing plate; 41. a right-angle groove; 42. an exhaust hole; 5. a first movable bar; 51. a second bar-shaped hole; 6. a bolt; 7. a bearing seat; 8. a lead screw; 9. a first motor; 10. a first motor case; 11. a sheet making base; 111. a carrying groove; 12. a film making table; 121. an Archimedes spiral groove; 13. a second movable bar; 14. a second motor; 15. a hollow cylindrical cam; 16. a third movable bar; 17. a hammer head; 18. a connecting cylinder; 19. a movable ring; 20. a C-shaped frame; 21. a handle; 22. a light gathering hole; 23. a spotlight.
Detailed Description
In order that the above objects, features and advantages of the present invention can be more clearly understood, the present invention will be further described with reference to the accompanying drawings and examples. It should be noted that the embodiments and features of the embodiments of the present application may be combined with each other without conflict. For convenience of description, the words "upper", "lower", "left" and "right" used herein refer to the same directions as the drawings, and do not limit the structure.
In the following description, numerous specific details are set forth in order to provide a thorough understanding of the present invention, however, the present invention may be practiced in other ways than those specifically described herein, and thus the present invention is not limited to the specific embodiments of the present disclosure.
In the embodiment, as shown in fig. 1, fig. 2, fig. 3, fig. 4 and fig. 5 (where archimedes' spiral grooves are not drawn), the present invention provides a device for preparing a cytological tissue specimen, which mainly comprises three parts, the first part is a console mainly composed of a stage 3 and a stage holder 1 for placing a slide and a cover glass, and the second part is a mechanical mechanism mainly composed of a second movable rod 13, a second motor 14, a hollow cylindrical cam 15, a third movable rod 16 and a hammer 17 for knocking moving bubbles; the third part is a transition mechanism consisting of a production station 12 and a production base 11 for combining the first and second parts.
Specifically, the first part of the cytological tissue sample slide preparation device provided by the invention comprises an object holder 1, wherein a support table 2 is arranged on the object holder 1, an object stage 3 is arranged on the support table 2, four first strip-shaped holes 31 and four right-angle fixing plates 4 are arranged on the upper surface of the object stage 3, the first strip-shaped holes 31 are radially distributed along the center of a light-gathering hole 22, two first movable rods 5 are symmetrically arranged on the lower surface of the object stage 3, two second strip-shaped holes 51 are arranged on the first movable rods 5, bolts 6 penetrating through the first strip-shaped holes 31 and the second strip-shaped holes 51 are arranged on the right-angle fixing plates 4, bearing seats 7 are arranged on two sides of the support table 2, the bearing seats 7 are connected with a lead screw 8, the lead screw 8 penetrates through the first movable rods 5 to be connected with a first motor 9, the first motor 9 is arranged in a first motor box 10, first motor case 10 sets up in the one side of carrying the thing seat, has two first motor casees promptly, the side of right angle fixed plate 4 is provided with exhaust hole 42, the inner wall of the side of right angle fixed plate 4 is provided with right angle groove 41.
According to the working principle of the first part of the invention, the first motor 9 is started to drive the screw rod 8 to rotate, and simultaneously the screw rod 8 drives the first movable rod 5 to horizontally push so that the right-angle fixing plate 4 is close to the center of the objective table 3 along the first strip-shaped hole 31 until the right-angle groove 41 presses the glass slide and the cover glass simultaneously, and the vent hole 42 in the right-angle fixing plate 4 can prevent bubbles from condensing when being pushed to the edges of the glass slide and the cover glass, so that gas can be discharged from the edges of the glass slide and the cover glass conveniently; the bearing arranged in the bearing seat 7 can provide a fulcrum for the rotation of the lead screw 8, the rotation friction force of the lead screw 8 is reduced, it needs to be explained that the switch of the first motor 9 belongs to a spring type contact switch, the switch can be conveniently started and stopped immediately, the regulation and control are convenient, and the sample pieces cannot be crushed or crushed.
The second and third sections provided by the present invention can be successfully implemented in order to achieve mechanical knock-out of the bubbles. Specifically, system piece seat 11 is gooseneck formula seat, be provided with the year thing groove 111 that is used for placing year thing seat 1 on the system piece seat 11, system piece platform 12 sets up the gooseneck head end at system piece seat 11 and is provided with archimedes helicla flute 121 on system piece platform 12, be provided with second movable rod 13 in the archimedes helicla flute 121, the bottom of second movable rod 13 is provided with second motor 14, the output of second motor 14 is provided with hollow cylinder cam 15, the inside of hollow cylinder cam 15 is provided with third movable rod 16, the top of third movable rod 16 is provided with the slip guide head, the inner wall of hollow cylinder cam 15 is provided with the track groove that supplies the slip guide head to remove, the bottom of third movable rod 16 is provided with tup 17. Be provided with connecting cylinder 18 on the second motor 14, hollow cylinder cam 15 sets up and forms certain protection to self in connecting cylinder 18, the outer wall of connecting cylinder 18 is provided with movable ring 19, movable ring 19 is connected with C type frame 20, C type frame 20 and third movable rod 16 fixed connection, the top of second movable rod 16 is provided with handle 21.
The third movable rod 16 in the second part is arranged inside the hollow cylindrical cam 15 so that the third movable rod 16 can move linearly along the axis of the hollow cylindrical cam 15, and thus, the real-time position of the handle 21 adjusted when rotating is the central position of the hammer 17, and if the handle is designed according to the existing cylindrical cam, the handle can deviate from the center of the cylindrical cam, which is not beneficial to adjustment; moreover, the movable ring 19 and the C-shaped frame 20 are used for controlling the third movable rod 16 not to do circular motion along with the hollow cylindrical cam 15, so that the motion track of the sliding guide head is ensured to be the track of the track groove, and further the effective linear reciprocating motion is ensured; functionally, the second and third portions are arranged such that the entire first portion mechanism is secured within the slide channel 111 to prevent horizontal movement when the slide and coverslip are knocked; the working process is that the second motor 14 is started to drive the hollow cylindrical cam 15 to rotate, the sliding guide head moves along the track groove, the third movable rod 16 and the hammer 17 do reciprocating linear motion, the handle 21 is manually moved, and the handle 21 drives the whole second part to do Archimedes spiral line motion along the Archimedes spiral groove 121. The position of the bubble is determined in the straight line for removing the bubble, preferably, the beating is started from the center of the sample, and the diameter of the hammer head 17 must be larger than the groove width of the archimedean spiral groove 121, otherwise, some bubbles are missed because of the groove interval of the archimedean spiral groove 121. Here, the maximum pole diameter of the archimedes spiral groove 121 needs to be set to be equal to or longer than the length of the slide glass, so that the large part of bubbles can be pushed out of the front side and the rear side of the slide glass when not pushed to the left and right maximum distances between the slide glass and the cover glass, bubbles are prevented from being accumulated and dispersed to the space between the slide glass and the cover glass at the end of bubble removal work, and the bubbles can be more fully polymerized and then pushed out according to the archimedes spiral motion.
In order to improve the quality of slide production, the invention provides that the center of the object stage 3 is provided with a light-gathering hole 22, the light-gathering hole 22 is provided with a flat glass capable of supporting the slide glass, and a spotlight 23 corresponding to the position of the light-gathering hole 22 is arranged inside the support stage 2 and can provide a light source, so that an operator can more finely observe bubbles remained in the sample until the bubbles cannot be observed.
On the whole, the device completes the work of removing the bubbles through machinery, reduces the manual workload, greatly reduces the number of the bubbles in the obtained sample, saves the time of student experiments, has excellent observation effect, and is reasonable in design, simple in structure and suitable for large-scale popularization.
The above description is only a preferred embodiment of the present invention, and not intended to limit the present invention in other forms, and any person skilled in the art may apply the above modifications or changes to the equivalent embodiments with equivalent changes, without departing from the technical spirit of the present invention, and any simple modification, equivalent change and change made to the above embodiments according to the technical spirit of the present invention still belong to the protection scope of the technical spirit of the present invention.
Claims (9)
1. A cell tissue sample slide making device is characterized by comprising an object holder and a slide making holder, wherein a supporting table is arranged on the object holder, an object stage is arranged on the supporting table, four first bar-shaped holes and four right-angle fixing plates are arranged on the upper surface of the object stage, two first movable rods are symmetrically arranged on the lower surface of the object stage, two second bar-shaped holes are arranged on the first movable rods, bolts penetrating through the first bar-shaped holes and the second bar-shaped holes are arranged on the right-angle fixing plates, bearing seats are arranged on two sides of the supporting table and are connected with a screw rod, the screw rod penetrates through the first movable rods and is connected with a first motor, the object holder is arranged on the slide making holder, the slide making table is arranged on the slide making holder, an Archimedes spiral groove is arranged on the slide making table, and a second movable rod is arranged in the Archimedes spiral groove, the bottom of second movable rod is provided with the second motor, the output of second motor is provided with hollow cylinder cam, the inside of hollow cylinder cam is provided with the third movable rod, the bottom of third movable rod is provided with the tup.
2. A device for preparing cytological tissue specimen, according to claim 1, wherein the second motor is provided with a connecting cylinder, the hollow cylindrical cam is arranged in the connecting cylinder, the outer wall of the connecting cylinder is provided with a movable ring, the movable ring is connected with a C-shaped frame, and the C-shaped frame is fixedly connected with a third movable rod.
3. A device for preparing a cytological tissue specimen, according to claim 2, wherein the top of the third movable rod is provided with a sliding guide head, and the inner wall of the hollow cylindrical cam is provided with a track groove for the sliding guide head to move.
4. A device for preparing a cytological tissue specimen according to claim 3, wherein the stage has a light-collecting hole at its center, a plate glass is arranged at the light-collecting hole, and a spotlight is arranged inside the support stage at a position corresponding to the light-collecting hole.
5. A device for preparing a cytological tissue specimen according to claim 4, wherein the first strip-shaped holes are radially distributed along the center of the light-focusing hole.
6. A device for preparing a cytological tissue specimen according to claim 5, wherein the first motor is arranged in a first motor case, and the first motor case is arranged at one side of the carrier base.
7. A device for preparing a cell tissue specimen according to claim 6, wherein a side surface of said right-angle fixing plate is provided with a vent hole, and an inner wall of the side surface of said right-angle fixing plate is provided with a right-angle groove.
8. A cytological tissue specimen slide preparation device according to claim 7, wherein the slide preparation base is a gooseneck base, and a slide groove for placing a slide holder is arranged on the slide preparation base.
9. A device for preparing a cytological tissue specimen according to claim 8, wherein a handle is provided on the top of the second movable rod.
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CN201810749044.2A CN109100189B (en) | 2018-07-10 | 2018-07-10 | Cell tissue sample film-making device |
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CN201810749044.2A CN109100189B (en) | 2018-07-10 | 2018-07-10 | Cell tissue sample film-making device |
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CN109100189B true CN109100189B (en) | 2020-12-04 |
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CN110673324B (en) * | 2019-09-27 | 2022-04-26 | 佛山科学技术学院 | Glass slide structure suitable for confocal microscope |
CN111909831B (en) * | 2020-08-07 | 2023-08-18 | 惠农达生物技术(江苏)有限公司 | Air exhausting device for solid state fermentation |
CN112345738B (en) * | 2020-10-26 | 2023-04-18 | 湖南中昊建设项目管理有限公司 | Slump meter |
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EP0440223B1 (en) * | 1990-01-31 | 1997-12-03 | Ladislav Novotny | Method of preparation of a capillary glass tube as miniaturized sensors |
CN103868779A (en) * | 2014-04-04 | 2014-06-18 | 黑龙江大学 | Method for cutting beet ovule and producing sheet in transparent condition |
ES2820833T3 (en) * | 2016-03-21 | 2021-04-22 | Tintometer Gmbh | Calibration suspension unit, procedure for producing a calibration suspension unit and use of a calibration suspension unit |
CN106092677B (en) * | 2016-05-30 | 2018-10-26 | 四川省农业科学院生物技术核技术研究所 | Sweet potato and its relative genus plant cell division phases sample fast preparation method |
CN107219111A (en) * | 2017-06-08 | 2017-09-29 | 黑龙江大学 | A kind of chromosome flaking method of distant hybridization beet |
CN206945405U (en) * | 2017-06-30 | 2018-01-30 | 迈克医疗电子有限公司 | Sample processing apparatus |
CN207263509U (en) * | 2017-09-28 | 2018-04-20 | 许昌学院 | A kind of tissue specimen fixing device for pathologic finding |
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Effective date of registration: 20240814 Address after: 1008, 10th Floor, Building 12, Futong West Street, Chaoyang District, Beijing 100102 Patentee after: Zhongsheng Tianjun (Beijing) Technology Co.,Ltd. Country or region after: China Address before: 252000 Wenxuan Middle School, Dongchangfu District, Liaocheng City, Shandong Province Patentee before: Jiao Mingyuan Country or region before: China |