CN109085090B - Method, measuring instrument and system for detecting specific gravity of urine - Google Patents
Method, measuring instrument and system for detecting specific gravity of urine Download PDFInfo
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- CN109085090B CN109085090B CN201810052721.5A CN201810052721A CN109085090B CN 109085090 B CN109085090 B CN 109085090B CN 201810052721 A CN201810052721 A CN 201810052721A CN 109085090 B CN109085090 B CN 109085090B
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N9/00—Investigating density or specific gravity of materials; Analysing materials by determining density or specific gravity
- G01N9/36—Analysing materials by measuring the density or specific gravity, e.g. determining quantity of moisture
Abstract
The invention provides a method for detecting the specific gravity of urine, which comprises the following steps: the measuring instrument applies a voltage to the electrochemical sensor; obtaining a response current value generated after the electrochemical sensor and urine act; and calculating the urine specific gravity of the urine according to the response current value. The application also provides a measuring instrument for detecting the urine specific gravity and a system for detecting the urine specific gravity. The urine specific gravity testing device tests the urine specific gravity by using an electrochemical method, and is high in precision, simple in required equipment and simple in operation.
Description
Technical Field
The invention relates to the technical field of urine detection, in particular to a method, a measuring system and a system for detecting urine specific gravity.
Background
Urine routine is a basic routine item for disease detection. The urine specific gravity and urine glucose in the urine routine can more intuitively realize the screening and diagnosis of diseases.
The specific gravity of urine itself reflects the solute content per volume of urine, which is the ratio of the weight of water at 4 ℃ to the weight of water of the same volume. The solute in urine mainly comprises metabolic micromolecules such as urea and the like, potassium, sodium, chlorine plasma and the like, and the higher the concentration of metabolites and other components in the urine is, the higher the urine specific gravity is. The specific gravity of normal adult urine is more than 1.015-1.025. The specific gravity of urine reflects the concentration function of kidney, and overhigh specific gravity of urine is usually seen in proteinuria, diabetes, acute nephritis and the like; hypo-specific gravity indicates impaired renal concentration function, which may be seen in chronic glomerulonephritis and uremia during polyuria.
The clinical test methods of the specific gravity of urine include a test paper method, a refractive index method and a weight method. The test paper method is the most common method for testing the specific gravity of urine by using a full-automatic urine analyzer in hospitals at present, is simple to operate but has low precision (+/-0.005), the refractive index method belongs to an optical method and is common in county and district hospitals, and the gravimetric method has high precision but is complex to operate and is common in laboratories and scientific research institutions.
Since the main substances influencing the specific gravity of urine in urine are small molecules such as urea and sodium ions, potassium ions and the like, the electrochemical electrode is considered to be feasible for measuring the current to reflect the specific gravity of urine.
Disclosure of Invention
The invention aims to provide a method for detecting the specific gravity of urine, which is used for detecting the specific gravity of the urine with higher accuracy.
In view of the above, the present application provides a method for detecting the specific gravity of urine, comprising:
the measuring instrument applies a voltage to the electrochemical sensor;
obtaining a response current value generated after the electrochemical sensor and urine act;
and calculating the urine specific gravity of the urine according to the response current value.
Preferably, the step of calculating the urine specific gravity of urine based on the response current value includes:
the urine specific gravity is calculated according to the following relation:
Y＝A*|X|+B；
wherein | X | represents an absolute numerical value of the response current value;
y is the urine specific gravity value;
the values of the parameters A and B are related to the reagent composition corresponding to the reagent layer in the electrochemical sensor.
Preferably, the reagent comprises:
the balance of pH buffer solution and water, and the mass ratio of the pH buffer solution to the water is 1: 1.
Preferably, the reagent specifically consists of: 6.45 wt% of potassium ferricyanide, 1.8 wt% of trehalose, 0.9 wt% of hydrophilic silica, 0.45 wt% of tween-20, 45.2 wt% of phosphoric acid buffer solution and 45.2 wt% of water, wherein Y is 0.00148X + 0.99922;
alternatively, the first and second electrodes may be,
the reagent specifically comprises the following components: 4 wt% of potassium ferricyanide, 3 wt% of trehalose, 1.5 wt% of hydrophilic silica, 0.3% of tween-20, 45.6 wt% of a phosphate buffer solution, and 45.6 wt% of water, wherein Y is 0.00183X + 1.00162.
Preferably, the voltage value of the applied voltage is +0.3V to + 0.5V.
Preferably, the step of obtaining the response current value generated after the electrochemical sensor reacts with urine specifically comprises:
and obtaining a response current value generated after the electrochemical sensor and urine act after a preset time.
Preferably, the predetermined time is 10s to 30 s.
Preferably, the step of applying a voltage to the electrochemical sensor by the measuring instrument comprises:
in response to an external trigger command, the measuring instrument applies a voltage to the electrochemical sensor;
alternatively, the first and second electrodes may be,
when the electrochemical sensor is detected, the measuring instrument applies a voltage to the electrochemical sensor.
The application also provides a measuring instrument for detecting the urine specific gravity, which comprises: the device comprises a voltage output module, a response current measuring module, a calculating module and a result output module;
the voltage output module is used for applying voltage to the electrochemical sensor;
the response current receiving module is used for obtaining a response current value generated after the electrochemical sensor and urine act;
the calculation module is used for calculating the urine specific gravity according to the relation between the response current value and the urine specific gravity;
the result output module is used for outputting the result of the urine specific gravity.
Preferably, the calculation module is further configured to calculate the urine specific gravity according to the following relationship:
Y＝A*|X|+B；
wherein | X | represents an absolute numerical value of the response current value;
y is the urine specific gravity value;
the values of the parameters A and B are related to the reagent composition corresponding to the reagent layer in the electrochemical sensor.
Preferably, the voltage output module is further used for responding to an external trigger command and applying voltage to the electrochemical sensor; alternatively, the voltage output module is further configured to apply a voltage to the electrochemical sensor when the electrochemical sensor is detected.
The application also provides a measuring instrument for detecting the urine specific gravity, which comprises: a memory, a processor and a computer program stored on the memory, the processor implementing the method as described in the above aspects when executing the computer program.
The present application also provides a non-transitory computer storage medium having stored thereon computer-executable instructions for causing a computer to perform the method as set forth in the above aspects.
The application also provides a system for detecting the urine specific gravity, which comprises the electrochemical sensor and the measuring instrument in the scheme.
The application provides a method for detecting urine specific gravity, which comprises the steps of firstly applying voltage to an electrochemical sensor, enabling the electrochemical sensor to act with urine, then obtaining response current generated after the electrochemical sensor acts with the urine, and finally calculating the urine specific gravity according to the response current. The detection method provided by the application can be used for completely realizing urine collection, reaction and result output, and is high in precision, simple in required equipment and simple in operation.
Drawings
FIG. 1 is a schematic illustration of a disassembled structure of an electrochemical sensor according to an embodiment of the present invention;
FIG. 2 is a schematic structural view of an electrochemical sensor according to the present invention;
FIG. 3 is a schematic view of an electrochemical sensor according to another embodiment of the present invention in a disassembled configuration;
FIG. 4 is a line graph of urine specific gravity versus electrochemical sensor test current as used in example 2;
FIG. 5 is a line graph of urine specific gravity versus electrochemical sensor test current as used in example 3.
Detailed Description
For a further understanding of the invention, reference will now be made to the preferred embodiments of the invention by way of example, and it is to be understood that the description is intended to further illustrate features and advantages of the invention, and not to limit the scope of the claims.
The inventor of the application finds out through experiments that: since the main substances in urine which affect the specific gravity of urine are small molecules such as urea and sodium ions and potassium ions, the electrochemical sensor can be used for measuring the specific gravity of urine.
The invention provides a method for detecting the urine specific gravity, which aims to solve the problem of low accuracy of the method for detecting the urine specific gravity in the prior art. Specifically, the embodiment of the invention discloses a method for detecting the specific gravity of urine, which comprises the following steps:
applying a voltage to the electrochemical sensor;
obtaining a response current value generated after the electrochemical sensor and urine act;
and calculating the urine specific gravity of the urine according to the response current value.
In the above process of detecting the specific gravity of urine, the measuring instrument applies a voltage to the electrochemical sensor, and according to the present invention, the above steps specifically include: in response to an external trigger command, the measuring instrument applies a voltage to the electrochemical sensor; or, when the electrochemical sensor is detected, the measuring instrument applies a voltage to the electrochemical sensor.
Specifically, the electrochemical sensor is detected by using the measuring instrument, namely the electrochemical sensor is connected with the measuring instrument, urine drops to the electrochemical sensor, and the urine specific gravity of the urine is tested by using the electrochemical sensor. When the electrochemical sensor is detected by the instrument to be measured, voltage is applied to the electrochemical sensor, the electrochemical sensor acts with urine, and the sensor reacts with and adsorbs substances in the urine. In certain embodiments, the measuring instrument applies a voltage to the electrochemical sensor of between +0.3V and + 0.5V.
And then the measuring instrument obtains the response current generated after the electrochemical sensor and the urine act, and finally the urine specific gravity is obtained according to a calculation module arranged in the measuring instrument. In some embodiments, the measuring instrument does not directly obtain the response current immediately, and the preset time is 10-30 s, namely the electrochemical sensor is acted on the urine to obtain the response current value. In the present application, the relational expression between the response current and the urine specific gravity in the calculation module arranged inside the measuring instrument is:
Y＝A*|X|+B；
wherein | X | represents an absolute numerical value of the response current value;
y is the urine specific gravity value;
the values of the parameters a and B are related to the reagent composition of the reagent layer in the electrochemical sensor.
The values of A and B in the relational expression are related to the reagent in the electrochemical sensor reagent layer, namely the reagent composition in the electrochemical sensor reagent layer is determined, and the values of A and B are correspondingly determined. In one particular embodiment, the reagents described herein comprise:
the balance of pH buffer solution and water, and the mass ratio of the pH buffer solution to the water is 1: 1.
In the above reagent, the electron mediator is ferricyanide, ferrocyanide, ferrocene or a derivative thereof, and in a specific embodiment, the electron mediator is ferricyanide; the content of the electron mediator is 2-10 wt%, and in a specific embodiment, the content of the electron mediator is 4-7 wt%; it directly affects the electron transport, detection range, response sensitivity and current magnitude in the reaction process.
The stabilizer is one or two of trehalose and chitosan, in a specific embodiment, the stabilizer is trehalose, and the content of the trehalose is 1-5 wt%; in a specific embodiment, the content of the stabilizer is 1.5-3.5 wt%.
The anti-agglomerating agent is one or two of hydrophilic silicon dioxide and xanthan gum, and in a specific embodiment, the anti-agglomerating agent is hydrophilic silicon dioxide and the content of the hydrophilic silicon dioxide is 0.5-2 wt%; in a specific embodiment, the content of the anti-agglomerating agent is 0.8-1.8 wt%. The anti-agglomerating agent serves to prevent agglomeration of the reagent layer.
The surfactant is selected from one or more of tween-20, tween-80 and triton-100, and in a specific embodiment, the surfactant is tween-20, and the content of the tween-20 is 0.1-0.5 wt%. The surfactant allows good contact between the aqueous reagent solution and the hydrophobic electrode.
The pH buffer is selected from one or more of phosphate buffer, MES buffer, and citrate buffer, and in particular embodiments, the pH buffer is phosphate buffer; the pH buffer is used to buffer reagent pH changes. In the reagent for detecting the urine specific gravity, the balance is pH buffer solution and water, and the mass ratio of the pH buffer solution to the water is 1: 1.
In one embodiment, the reagent layer corresponds to reagents in weight percentages of 6.45% potassium ferricyanide, 1.8% trehalose, 0.9% hydrophilic silica, tween-200.45%, 45.2% phosphate buffer, and 45.2% water, thereby obtaining the relationship: Y0.00148X + 0.99922;
in one embodiment, the reagent layer corresponds to reagents in weight percentages of 4% potassium ferricyanide, 3% trehalose, 1.5% hydrophilic silica, 200.3% tween, 45.6% phosphate buffer solution, and 45.6% water, thereby obtaining the relationship: Y0.00183X + 1.00162.
It will be understood by those skilled in the art that the foregoing is merely exemplary and that the coefficients a and B will vary if the weight percentages of the components are adjusted accordingly within the ranges of the components. In addition, if the sensor structure or shape is changed, the coefficients a and B are also changed.
By utilizing the relational expression, after the response current is obtained, the test system can directly calculate the specific gravity of the urine according to the response current.
In the actual process of detecting the specific gravity of urine, one alternative is to put the sensor into a measuring instrument and then sample the urine, i.e. drop the urine into or add the urine into the sensor containing the reagent layer, and the measuring instrument applies a voltage, e.g. +0.3V to +0.5V, to the sensor, measures the magnitude of the response current after a predetermined time, e.g. measures the response current at any time within 10s to 30s, and directly calculates the specific gravity value according to the response current by a chip or a calculating unit inside the measuring instrument.
Or dropping urine into the sensor containing the reagent layer, placing the sensor into a measuring instrument, applying a voltage to the sensor by the measuring instrument, and measuring a response current after a preset time; the starting point of the predetermined time may be a time when the measuring instrument starts to detect the response current, that is, a starting time when the measuring instrument detects the response current. The starting point of the specific implementation can also be other conveniently recorded moments such as the moment of applying voltage or the moment of completing sample application.
It should be noted that the electrochemical sensor is sometimes referred to as an electrochemical test strip by those skilled in the art.
On the basis of the method for detecting the specific gravity of the urine, the application also provides a measuring instrument for detecting the specific gravity of the urine, which comprises the following steps: the device comprises a voltage output module, a response current receiving module, a calculating module and a result output module;
the voltage output module is used for applying voltage to the electrochemical sensor;
the response current receiving module is used for obtaining a response current value generated after the electrochemical sensor and urine act;
the calculation module is used for calculating the urine specific gravity according to the relation between the response current value and the urine specific gravity;
the result output module is used for outputting the result of the urine specific gravity.
The voltage output module, the response current receiving module, the calculating module and the result output module are specific module forms well known to those skilled in the art, and the application is not particularly limited; however, the calculation module is a calculation program designed according to the specific requirements of the application, namely the urine specific gravity can be directly obtained through the measured electrode current only by fitting the urine specific gravity with the electrode current output by the measuring instrument.
The calculation process is limited to the internal setting of the measuring instrument; in practical application, urine drops to the electrochemical sensor, and the measuring instrument applies voltage and then can directly obtain a urine specific gravity value from a measuring instrument result output module such as a display screen.
The present application also provides a measurement instrument, which includes: a memory, a processor and a computer program stored on the memory, the processor when executing the computer program implementing the above method of detecting the specific gravity of urine.
The present application also provides a non-transitory computer storage medium having stored thereon computer-executable instructions for causing a computer to perform the above-described method of detecting a specific gravity of urine.
The invention provides a system for detecting the specific gravity of urine, which comprises an electrochemical sensor and a measuring instrument, wherein the measuring instrument can be any one of the implementation modes given in the embodiment. The electrochemical sensor acts with urine after sample adding, a circuit path is formed after the measuring instrument provides voltage, and the measuring instrument calculates the urine specific gravity and outputs the urine specific gravity by measuring the response current value.
Specifically, the schematic structural diagram of the electrochemical sensor is shown in fig. 1 or fig. 2, in which 1 is a substrate, 2 is a working electrode, 3 is a counter electrode, 4 is a trigger electrode, 5 is an insulating film, 6 is a reagent layer, 7 is a hydrophilic film, 8 is an adhesive layer, 9 is a sample guide groove, and 10 is an air outlet;
a plurality of electrodes are arranged on the substrate;
an insulating film is arranged on the surface of at least one electrode, an opening is formed in the insulating film, and a reagent layer is arranged in the opening;
an adhesive layer and a hydrophilic film are sequentially arranged on the surface of the insulating film along the direction far away from the substrate;
the bonding layer is provided with a sample guide groove, the hydrophilic film is provided with an air outlet, part or all of the sample guide groove is over against the reagent layer, and the air outlet is over against the sample guide groove.
The shape of the electrode is not particularly limited in this application, and may be a shape known to those skilled in the art. In a specific embodiment, the electrodes comprise a trigger electrode, a working electrode and a counter electrode, the working electrode is L-shaped, and the counter electrode is L-shaped; the L end of the working electrode and the L end of the counter electrode are both arranged at one end adjacent to the reagent layer, and the trigger electrode is arranged in a space formed by the working electrode and the counter electrode.
An insulating film is provided on the surface of the L-end of the working electrode to limit the area of the reagent layer. A plurality of openings, for example two or three openings, may be provided in the insulating film, with one reagent layer being provided in the opening adjacent to the L-end of the working electrode. If the number of the openings is two, one opening is over against the L end of the working electrode, and the other opening is over against the L end of the counter electrode; if the number of the openings is three, one of the openings is just opposite to the L end of the working electrode, and the other two openings are respectively just opposite to the L end of the counter electrode and the T end of the trigger electrode. The hydrophilic film and the bonding layer are both on the same side as the insulating film. The hydrophilic film is a complete PET film and forms a sample guide groove with the bonding layer, namely the bonding layer is provided with the sample guide groove, and the hydrophilic film is provided with an air outlet within the range of the sample guide groove and serves as an air outlet for siphon sample introduction. The length of the hydrophilic membrane or the adhesive layer may be equal, for example one third or one half of the length of the trigger electrode, ensuring that a portion of each of the three electrodes is located directly below the adhesive layer and the hydrophilic membrane. After entering the sensor from the sample guide groove, the liquid sample such as urine comes into contact with the counter electrode, the trigger electrode and the reagent layer. In the application, the T end of the trigger electrode represents a T-shaped top end, and the L ends of the working electrode and the counter electrode both represent L-shaped ends with bends.
The application provides a system and a method for detecting urine specific gravity, which test the urine specific gravity by an electrochemical method; the test method is simple and has little need for urine samples, generally speaking, the urine amount only needs 2 microliter to accurately measure the specific gravity of urine.
For further understanding of the present invention, the method for detecting the specific gravity of urine provided by the present invention is described in detail with reference to the following examples, and the scope of the present invention is not limited by the following examples.
Example 1
A system for detecting the specific gravity of urine and urine comprises an electrochemical sensor and a measuring instrument, wherein figure 1 is a structural schematic diagram of an optional electrochemical sensor, and figure 1 is a substrate, 2 is a working electrode, 3 is a counter electrode, 4 is a trigger electrode, 5 is an insulating film, 6 is a reagent layer, 7 is a hydrophilic film, 8 is a double-sided adhesive layer, 9 is a sample guide groove, and 10 is an air outlet;
the surface of the substrate is printed with an electrode shape by screen printing, and the electrode shape sequentially comprises an L-shaped working electrode 2, an L-shaped counter electrode 3 and a T-shaped trigger electrode 4, wherein the trigger electrode 4 is arranged in a space formed by the working electrode 2 and the counter electrode 3;
the L end side surface of the working electrode 2 is provided with an insulating film 5, the insulating film 5 is provided with an opening, and the reagent layer 6 is provided in the opening;
a double-sided adhesive layer 8 and a hydrophilic film 7 are sequentially arranged on the surface of the insulating film 5 along the direction far away from the substrate 1;
the double-sided adhesive layer 8 is provided with a sample guide groove 9, the hydrophilic film 7 is provided with a gas outlet 10, part or all of the sample guide groove 9 is over against the reagent layer 6, and the gas outlet 10 is over against the sample guide groove 9.
In addition to this sensor, other sensors may be employed, and as shown in fig. 3, the opening of the insulating film is plural, for example, two, and the reagent layer 6 is provided in the opening facing the working electrode.
The measurement instrument provided by this implementation includes: the device comprises a voltage output module, a response current receiving module, a calculating module and a result output module;
the voltage output module is used for applying voltage to the electrochemical sensor;
the response current receiving module is used for obtaining a response current value generated after the electrochemical sensor and urine act;
the calculation module is used for calculating the urine specific gravity according to the relation between the response current value and the urine specific gravity;
the result output module is used for outputting the result of the urine specific gravity.
Example 2
The specific gravity of urine is measured by using the system of example 1, the reagents in the reagent layer of the sensor are shown in table 1, and table 1 is a data table of the composition of the reagents in the reagent layer of this example; thus, a calculation formula Y-0.00148 × X +0.99922 provided in the measuring instrument is obtained; the values of the parameters a and B can be determined by a number of experiments.
Table 1 table of reagent data for reagent layer of example 2
| Substance(s) | Mass ratio of |
| Potassium ferricyanide | 6.45％ |
| Trehalose | 1.8％ |
| Hydrophilic silica | 0.9％ |
| Tween-20 | 0.45％ |
| Phosphoric acid buffer solution | 45.2％ |
| Water (W) | 45.2％ |
10 urine samples were sampled, numbered 1 to 10, respectively, and a +0.4V voltage was applied to the sensor using a measuring instrument to measure a response current for 30 seconds, and the urine specific gravity was directly obtained from the measuring instrument side by the above calculation formula set in the measuring instrument based on the response current, and at the same time, the urine specific gravity of each urine sample was measured using a refractometer, and the calculated urine specific gravity calculated by the electrode current was compared with the urine specific gravity measured using the refractometer to calculate an error, and the results are shown in Table 2.
Table 2 error data table of urine specific gravity measured by the urine specific gravity and refractometer in this embodiment
| Urine sample numbering | Electrode current (μ A) | Specific gravity of output urine | Measuring specific gravity of urine | Error of urine |
| 1 | 21.94 | 1.032 | 1.03 | 0.002 |
| 2 | 18.59 | 1.027 | 1.025 | 0.002 |
| 3 | 22.24 | 1.032 | 1.034 | -0.002 |
| 4 | 17.18 | 1.025 | 1.026 | -0.001 |
| 5 | 4.279 | 1.006 | 1.005 | 0.001 |
| 6 | 4.152 | 1.005 | 1.005 | 0.000 |
| 7 | 12.87 | 1.018 | 1.018 | 0.000 |
| 8 | 9.594 | 1.013 | 1.015 | -0.002 |
| 9 | 17.8 | 1.026 | 1.022 | 0.004 |
| 10 | 24.1 | 1.035 | 1.03 | 0.005 |
The result of calculating the urine specific gravity by using the electrode current is superior to the test result of the urine test paper (the adjacent gradient phase difference of the urine specific gravity of the urine test paper is 0.005), and compared with the test result of a more accurate refractometer, the result also has high comparability.
The relationship graph of the urine specific gravity and the electrode current is shown in fig. 4, and fig. 4 is a fitting line graph of the urine specific gravity and the electrochemical sensor test current; as can be seen from FIG. 4, the urine specific gravity and the electrode current (30s) were linearly fitted to a curve R2A linear relationship is better at 0.9802.
Example 3
The procedure for measuring the specific gravity of urine was the same as in example 2, except that the reagents of the sensor reagent layer were calculated according to the following formula, as shown in table 3: Y0.00183X + 1.00162; the test results and the comparison results are shown in table 4, and fig. 5 is a linear graph of the urine specific gravity and the test current of the electrochemical sensor according to the present embodiment;
table 3 reagent data table of reagent layer of sensor of this embodiment
| Substance(s) | Mass ratio of |
| |
4 |
| Trehalose | |
| 3％ | |
| Hydrophilic silica | 1.5％ |
| Tween-20 | 0.3％ |
| Phosphoric acid buffer solution | 45.6％ |
| Water (W) | 45.6％ |
Table 4 error data table of urine specific gravity and refractometer testing urine specific gravity obtained in this example
| Urine sample numbering | Electrode current (μ A) | Calculating the specific gravity of urine | Measuring specific gravity of urine | Error of urine |
| 1 | 15.62 | 1.030 | 1.029 | 0.001 |
| 2 | 8.44 | 1.017 | 1.02 | -0.003 |
| 3 | 3.33 | 1.008 | 1.006 | 0.002 |
It is understood that when the composition ratio of the reagent is changed, the parameters A and B are changed accordingly, and those skilled in the art can change the composition ratio of the reagent and obtain the corresponding parameter values based on the change.
The above description of the embodiments is only intended to facilitate the understanding of the method of the invention and its core idea. It should be noted that, for those skilled in the art, it is possible to make various improvements and modifications to the present invention without departing from the principle of the present invention, and those improvements and modifications also fall within the scope of the claims of the present invention.
The previous description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the invention. Thus, the present invention is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.
Claims (12)
1. A method of measuring urine specific gravity comprising:
the measuring instrument applies a voltage to the electrochemical sensor;
obtaining a response current value generated after the electrochemical sensor and urine act;
calculating urine specific gravity according to the response current value;
the step of calculating the urine specific gravity of the urine according to the response current value comprises the following steps:
the urine specific gravity is calculated according to the following relation:
Y＝A*|X|+B；
wherein | X | represents an absolute numerical value of the response current value;
y is the urine specific gravity value;
the values of the parameters A and B are related to reagent compositions corresponding to a reagent layer in the electrochemical sensor, and are obtained by fitting the urine specific gravity and the test current of the electrochemical sensor.
2. The method of claim 1, wherein the reagent comprises:
2-10 wt% of an electron mediator;
1-5 wt% of a stabilizer;
0.5-2 wt% of an anti-coagulant;
0.1-0.5 wt% of a surfactant;
the balance of pH buffer solution and water, and the mass ratio of the pH buffer solution to the water is 1: 1.
3. The method according to claim 2, wherein the reagent is specifically composed of: 6.45 wt% of potassium ferricyanide, 1.8 wt% of trehalose, 0.9 wt% of hydrophilic silica, 0.45 wt% of tween-20, 45.2 wt% of phosphoric acid buffer solution and 45.2 wt% of water, wherein Y is 0.00148X + 0.99922;
alternatively, the first and second electrodes may be,
the reagent specifically comprises the following components: 4 wt% of potassium ferricyanide, 3 wt% of trehalose, 1.5 wt% of hydrophilic silica, 0.3% of tween-20, 45.6 wt% of a phosphate buffer solution, and 45.6 wt% of water, wherein Y is 0.00183X + 1.00162.
4. The method according to claim 1, wherein the voltage value of the applied voltage is +0.3V to + 0.5V.
5. The method according to claim 1, wherein the step of obtaining the response current value generated by the electrochemical sensor after the interaction with urine is specifically:
and obtaining a response current value generated after the electrochemical sensor and urine act after a preset time.
6. The method of claim 5, wherein the predetermined time is 10s to 30 s.
7. The method of claim 1, wherein the step of applying a voltage to the electrochemical sensor by the measuring instrument comprises:
in response to an external trigger command, the measuring instrument applies a voltage to the electrochemical sensor;
alternatively, the first and second electrodes may be,
when the electrochemical sensor is detected, the measuring instrument applies a voltage to the electrochemical sensor.
8. A measuring instrument for measuring the specific gravity of urine, comprising: the device comprises a voltage output module, a response current measuring module, a calculating module and a result output module;
the voltage output module is used for applying voltage to the electrochemical sensor;
the response current receiving module is used for obtaining a response current value generated after the electrochemical sensor and urine act;
the calculation module is used for calculating the urine specific gravity according to the relation between the response current value and the urine specific gravity;
the result output module is used for outputting the result of the urine specific gravity;
the calculation module is further used for calculating the urine specific gravity according to the following relational expression:
Y＝A*|X|+B；
wherein | X | represents an absolute numerical value of the response current value;
y is the urine specific gravity value;
the values of the parameters A and B are related to reagent compositions corresponding to a reagent layer in the electrochemical sensor, and are obtained by fitting the urine specific gravity and the test current of the electrochemical sensor.
9. The measurement instrument of claim 8, wherein the voltage output module is further configured to apply a voltage to the electrochemical sensor in response to an external trigger command; alternatively, the voltage output module is further configured to apply a voltage to the electrochemical sensor when the electrochemical sensor is detected.
10. A measuring instrument for detecting the specific gravity of urine and urine is characterized by comprising: memory, a processor and a computer program stored on the memory, the processor implementing the method according to any of claims 1 to 7 when executing the computer program.
11. A non-transitory computer storage medium storing computer-executable instructions for causing a computer to perform the method of any one of claims 1 to 7.
12. A system for measuring the specific gravity of urine comprising an electrochemical sensor and a measuring instrument according to any one of claims 8 to 10.
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| CN110841120A (en) * | 2019-11-26 | 2020-02-28 | 耿怀振 | Special urethral catheterization device for urinary surgery in hospital, control method and control system |
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| JPH0587806A (en) * | 1991-09-26 | 1993-04-06 | Stec Kk | Urine analyzing method |
| CN101393200A (en) * | 2007-09-21 | 2009-03-25 | 五鼎生物技术股份有限公司 | Electrochemical quantitative analysis system and method |
| CN102565153A (en) * | 2011-12-27 | 2012-07-11 | 桂林乐尔医疗器械有限公司 | Electrode type urine glucose testing strip with function of pretreating detected sample |
| CN103048219A (en) * | 2012-12-05 | 2013-04-17 | 清华大学 | Analytical method of iodine-containing hydriodic acid concentration |
| CN103983669A (en) * | 2013-02-08 | 2014-08-13 | 昀尚有限公司 | Detection test piece, detection device and detection method |
| JP2017527818A (en) * | 2014-09-15 | 2017-09-21 | ステレンボッシュ ユニバーシティ | Method and system for determining fluid density |
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| JPH0587806A (en) * | 1991-09-26 | 1993-04-06 | Stec Kk | Urine analyzing method |
| CN101393200A (en) * | 2007-09-21 | 2009-03-25 | 五鼎生物技术股份有限公司 | Electrochemical quantitative analysis system and method |
| CN102565153A (en) * | 2011-12-27 | 2012-07-11 | 桂林乐尔医疗器械有限公司 | Electrode type urine glucose testing strip with function of pretreating detected sample |
| CN103048219A (en) * | 2012-12-05 | 2013-04-17 | 清华大学 | Analytical method of iodine-containing hydriodic acid concentration |
| CN103983669A (en) * | 2013-02-08 | 2014-08-13 | 昀尚有限公司 | Detection test piece, detection device and detection method |
| JP2017527818A (en) * | 2014-09-15 | 2017-09-21 | ステレンボッシュ ユニバーシティ | Method and system for determining fluid density |
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