CN111781096A - Correction method of panda urine hormone - Google Patents
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- G01N9/00—Investigating density or specific gravity of materials; Analysing materials by determining density or specific gravity
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- G01N33/74—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving hormones or other non-cytokine intercellular protein regulatory factors such as growth factors, including receptors to hormones and growth factors
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Abstract
The invention discloses a correction method of panda urine hormone, which comprises the following steps: collecting panda urine; measuring the original hormone concentration and the urine specific gravity of the panda urine; correcting the original hormone concentration according to the correction result, namely the original hormone concentration/Lg urine specific gravity/500000 to obtain a correction result; the invention directly adopts function conversion to the urine specific gravity value measured by the panda urine and then carries out hormone urine specific gravity correction, eliminates the problem of insufficient representativeness existing in the average urine specific gravity value, does not need to carry out urine collection and calculation on the average urine specific gravity value in advance, only needs to carry out urine specific gravity measurement on specific pandas and then directly corrects the hormone level by using a formula, and the correction method has simple operation, quick correction and good accuracy.
Description
Technical Field
The invention relates to the technical field of correction of panda urine hormones, in particular to a correction method of panda urine hormones.
Background
The concentration of substances in urine is influenced by drinking water, urine volume, food and other factors, and in order to eliminate the influence, the method can be used for correcting by a 24-hour urine collecting method, a flow rate correcting method, a creatinine correcting method and a specific gravity correcting method, but the 24-hour urine collecting method and the flow rate correcting method are inconvenient to operate and are less adopted.
In the management of captive pandas, the levels of reproductive hormones such as estrogen, progestogen and the like in urine samples of corresponding individual pandas in different time periods are detected, and the changes of the relevant hormones such as estrogen, progestogen and the like in each time period are contrastively analyzed, so that whether the corresponding pandas estrus or not and the stage of estrus are judged, and further, specific feeding, reproduction management and other implementation schemes are formulated in the management. However, the concentration of hormones in the urine of pandas is influenced by the above factors, and in order to make the hormone levels in the urine of different concentrations comparatively analyzable, the hormone levels are corrected by creatinine in the actual hormone measurement, and the change of estrogen, progestogen and the like in the urine of the pandas at different time points is comparatively analyzed according to the results of the hormones corrected by the creatinine.
The creatinine correction result of the panda urine hormone plays a great role in panda management and is widely applied to a panda core population management unit; however, mechanisms such as Chinese panda breeding and exhibition and the like are provided with few pandas, usually 2-4 pandas, and corresponding detection departments are not arranged for measuring the creatinine of the panda urine; on the other hand, the chemical reagents required for creatinine determination include: picric acid, trinitrophenol, is a flammable and explosive agent that requires special approval from the local police department for purchase and transport; the other is sodium hydroxide which is strong alkali, and except for scientific research requirements, the chemical reagents are not purchased by institutions such as general panda breeding and exhibition; therefore, in the breeding season of pandas every year, a panda adult breeding research base sends hormone testers to the panda breeding and developing institution in China for hormone measurement. However, the reagent for measuring creatinine, which carries picric acid, is limited. Therefore, in the aspect of correction of the urine hormone creatinine of the pandas, domestic related panda feeding mechanisms can only resort to local colleges and universities or hospitals, as the colleges and the hospitals are usually far away from the feeding mechanisms, a large amount of time is spent on sample feeding, or as the colleges and the hospital staff cannot timely detect the urine creatinine content due to work arrangement, the correction result of the urine hormone cannot be timely obtained.
Currently, Jella Wauters (2018) reports correction of uropycnometry for urohormone levels in pandas, the correction formula is: and the hormone urine specific gravity correction result is original hormone concentration x [ (average urine specific gravity value-1.000)/(target sample urine specific gravity value-1.000) ], and the average urine specific gravity value refers to an average urine specific gravity value calculated after detecting not less than 30 urine samples collected from a specific panda individual. In the formula, before hormone urine specific gravity correction is carried out on a specific panda individual, the average urine specific gravity value of the individual in the past needs to be obtained. Because the urine concentration and the urine output of pandas are affected by the ingestion of pandas (for example, the ingestion of different types of bamboos in different seasons and different parts of the bamboos such as bamboo stems, bamboo leaves and bamboo shoots), the amount of exercise and physiological changes in estrus and rest periods, the drinking conditions and the like, the average value of the urine specific gravity calculated by collecting no less than 30 urine samples for detection in the report is not enough to represent the real average value of the urine specific gravity of a particular individual panda in each season and various physiological conditions. If a representative average urine specific gravity value needs to be obtained, the average urine specific gravity value needs to be acquired and measured for a specific panda in different seasons, different foods, different physiological conditions and the like, so that long-term urine acquisition and urine specific gravity detection can be performed on the panda. Meanwhile, in the management of pandas, the hormone level is often temporarily detected and corrected for a specific individual, and for pandas for which urine sample collection and average urine specific gravity value calculation have not been performed in advance, the hormone correction formula cannot be adopted.
Disclosure of Invention
Aiming at the defects in the prior art, the invention provides a correction method of panda urine hormone, which is simple to operate, quick to correct and good in accuracy.
In order to achieve the above purpose of the present invention, the following technical solutions are adopted:
the invention provides a correction method of panda urine hormone, which comprises the following steps:
(a) collecting panda urine;
(b) measuring the original hormone concentration and the urine specific gravity of the panda urine;
(c) the original hormone concentration was corrected by the correction result of original hormone concentration/Lg urine specific gravity/500000 to obtain a correction result.
The invention directly adopts function conversion to the urine specific gravity value measured by the panda urine and then carries out hormone urine specific gravity correction, thereby eliminating the problem of insufficient representativeness existing in the average urine specific gravity value, and the urine collection and the calculation of the average urine specific gravity value are not needed to be carried out on the specific panda in advance; specifically, the correlation r of the correction method and the creatinine correction method to the estrogen correction result is 0.968, and the correlation r to the progestogen correction result is 0.98.
The correction result of the present invention, original hormone concentration/Lg specific gravity of urine/500000, is specifically: corrected result-original hormone concentration divided by Lg urine specific gravity, divided by 500000; wherein the specific gravity of Lg is logarithmic of the specific gravity value of urine with 10 as a base.
Preferably, the hormone is an estrogen and/or a progestin.
The method for measuring the specific gravity of urine in the present invention is not limited strictly, and a conventional method for measuring the specific gravity of urine in the art may be selected according to actual needs.
More preferably, the hydrometer method determining the specific gravity of urine comprises:
the urine hydrometer is checked and corrected, then the urine hydrometer of the panda urine is measured, and after the measurement is finished, the urine hydrometer is cleaned.
The urine hydrometer is checked and corrected firstly, so that the accuracy of the urine hydrometer value measuring result can be guaranteed, and the accuracy of urine hormone correction is further guaranteed better.
Preferably, the verification correction comprises:
deionized water is added into a sample groove of the uropycnometer, and the deionized water in the sample groove is sucked out when the reading of the uropycnometer is fixed to be 1.000.
Preferably, the cleaning solution adopted by the cleaning is deionized water, and the reading of the uropycnometer is fixed to be 1.000 after the deionized water is added.
The method for determining the original hormone concentration in the panda urine is not strictly limited, and the conventional determination method of the hormone in the urine in the field can be adopted, for example, the content of the hormone in the panda urine can be determined by adopting an enzyme-linked immunosorbent assay; specifically, the method for detecting the original progestogen content in the urine of the panda disclosed in the specific embodiment of the chinese patent application with the patent application number CN201310092282.8 can be adopted; the method for detecting the original estrogen content in the urine of the panda can be adopted, which is disclosed in the specific embodiment of the Chinese patent application with the patent application number of 201310092471.5.
Preferably, the panda urine collection method comprises the following steps:
cleaning and drying the ground of the animal house;
putting the pandas into a animal house, collecting urine by using an injector once the pandas discharge urine, filling the urine into a freezing pipe, and immediately putting the freezing pipe into a refrigerator for freezing or refrigerating.
By adopting the method to collect the panda urine, the panda can be prevented from being polluted, and the urine can be exposed in the air for a long time to influence the content of each substance in the urine.
The invention has the beneficial effects that:
the invention directly adopts function conversion to the urine specific gravity value measured by the panda urine and then carries out hormone urine specific gravity correction, thereby eliminating the problem of insufficient representativeness existing in the average urine specific gravity value, and the urine collection and the calculation of the average urine specific gravity value are not needed to be carried out on the specific panda in advance; specifically, the correlation r of the correction method and the creatinine correction method to the estrogen correction result is 0.968, and the correlation r to the progestogen correction result is 0.98.
Drawings
In order to more clearly illustrate the detailed description of the invention or the technical solutions in the prior art, the drawings that are needed in the detailed description of the invention or the prior art will be briefly described below. Throughout the drawings, like elements or portions are generally identified by like reference numerals. In the drawings, elements or portions are not necessarily drawn to scale.
FIG. 1 is a correlation analysis of correction results of estrogen in panda urine by creatinine correction and urine specific gravity correction in the experimental example of the present invention;
FIG. 2 is a correlation analysis of the correction results of progestogen in panda urine by creatinine correction and urine specific gravity correction in the experimental example of the present invention.
Detailed Description
Embodiments of the present invention will be described in detail below with reference to the accompanying drawings. The following examples are only for illustrating the technical solutions of the present invention more clearly, and therefore are only examples, and the protection scope of the present invention is not limited thereby.
It is to be noted that, unless otherwise specified, technical or scientific terms used herein shall have the ordinary meaning as understood by those skilled in the art to which the invention pertains.
Example 1
The embodiment is a correction method of urine estrogen of pandas, which comprises the following steps:
(a) cleaning and drying the ground of the animal house; putting pandas into a animal house, collecting urine by using an injector once the pandas discharge urine, filling the urine into a freezing pipe, and immediately putting the freezing pipe into a refrigerator for refrigeration;
(b) detecting the original estrogen concentration in the panda urine by using the original estrogen concentration detection method in the panda urine disclosed in the specific embodiment of the patent application with the application number of 201310092471.5; and measuring urine specific gravity of the panda urine by using a urine specific gravity meter (model PAL-10S, brand ATAGO);
(c) the original estrogen concentration was corrected according to the correction result of original estrogen concentration/Lg urine specific gravity/500000 to obtain an estrogen correction result.
Example 2
The embodiment is a correction method of urine progestogen of a panda, which comprises the following steps:
(a) cleaning and drying the ground of the animal house; putting pandas into a animal house, collecting urine by using an injector once the pandas discharge urine, filling the urine into a freezing pipe, and immediately putting the freezing pipe into a refrigerator for refrigeration;
(b) detecting the original progestogen concentration in the panda urine by adopting the method for detecting the original progestogen concentration in the panda urine disclosed in the specific embodiment of the patent application with the application number of CN 201310092282.8; and measuring urine specific gravity of the panda urine by using a urine specific gravity meter (model PAL-10S, brand ATAGO);
(c) the original progestogen concentration is corrected in accordance with the correction result of original progestogen concentration/Lg urine specific gravity/500000 to obtain a progestogen correction result.
Comparative example 1
The comparison example is a correction method of urine estrogen of pandas, which comprises the following steps:
(a) cleaning and drying the ground of the animal house; putting pandas into a animal house, collecting urine by using an injector once the pandas discharge urine, filling the urine into a freezing pipe, and immediately putting the freezing pipe into a refrigerator for refrigeration;
(b) detecting the original estrogen concentration in the panda urine by using the original estrogen concentration detection method in the panda urine disclosed in the specific embodiment of the patent application with the application number of 201310092471.5; and detecting the concentration of creatinine in the panda urine by using the method for detecting the concentration of creatinine in the panda urine disclosed in the specific embodiment in the patent application with the application number of 201310092471.5;
(c) and correcting the original estrogen according to the correction result, namely the original estrogen concentration/creatinine concentration to obtain an estrogen correction result.
Comparative example 2
The comparison example is a correction method of urine progestogen of pandas, which comprises the following steps:
(a) cleaning and drying the ground of the animal house; putting pandas into a animal house, collecting urine by using an injector once the pandas discharge urine, filling the urine into a freezing pipe, and immediately putting the freezing pipe into a refrigerator for refrigeration;
(b) detecting the original progestogen concentration in the panda urine by adopting the method for detecting the original progestogen concentration in the panda urine disclosed in the specific embodiment of the patent application with the application number of CN 201310092282.8; and detecting the concentration of creatinine in the panda urine by using a method for detecting the concentration of creatinine in the panda urine disclosed in the specific embodiment of the patent application with the application number of CN 201310092282.8; (ii) a
(c) And correcting the original progestogen according to the correction result, namely the original progestogen concentration/creatinine concentration, so as to obtain a progestogen correction result.
Examples of the experiments
Collecting urine of pandas in different periods of time in the period of budding and oestrus in a Chengdu panda breeding research base, respectively correcting estrogen and progestogen values in the panda urine by adopting the correction methods (namely, a creatinine correction method and a urine specific gravity correction method) in the embodiments 1-2 and the comparison examples 1-2, and obtaining correction results shown in the following table 1;
TABLE 1 correction of hormones by different correction methods
Carrying out correlation analysis on the results of creatinine correction method and urine specific gravity correction method of estrogen and progestogen; the analysis results are shown in fig. 1 and fig. 2, wherein Ec/USG refers to the specific gravity correction result of estrogen urine of the same panda in the process of carrying out the same panda in the form of sprouting urine by adopting the method; Ec/Cr refers to the result of correcting the estrogen creatinine of the urine of the same giant panda in sprouting by adopting a creatinine method; the Pg/USG refers to the specific gravity correction result of the progestogen in the same panda germinating urine by adopting the method; Pg/Cr refers to a result of correcting progestational hormone creatinine in the same giant panda when the giant panda sprouts by adopting a creatinine method;
as can be seen from fig. 1, the correlation coefficient r of the calibration result of estrogen by the urine specific gravity calibration method and the creatinine calibration method of the present application is 0.968; therefore, the urine specific gravity correction method has good correlation degree with the creatinine correction method, and has higher accuracy on estrogen.
As can be seen from fig. 2, the correlation coefficient r of the results of the urogravity calibration method and the creatinine calibration method for the progestogen calibration is 0.98; therefore, the urine specific gravity correction method has good correlation degree with the creatinine correction method, and has higher accuracy on the progestogen.
The above is only a partial experimental result in a large number of studies by the applicant, but it can be stated that:
the invention directly adopts function conversion to the urine specific gravity value measured by the panda urine and then carries out hormone urine specific gravity correction, eliminates the problem of insufficient representativeness existing in the average urine specific gravity value, does not need to carry out urine collection and calculation on the average urine specific gravity value in advance, only needs to carry out urine specific gravity measurement on specific pandas and then directly corrects the hormone level by using a formula, and the correction method has simple operation, quick correction and good accuracy.
Finally, it should be noted that: the above embodiments are only used to illustrate the technical solution of the present invention, and not to limit the same; while the invention has been described in detail and with reference to the foregoing embodiments, it will be understood by those skilled in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some or all of the technical features may be equivalently replaced; such modifications and substitutions do not depart from the spirit and scope of the present invention, and they should be construed as being included in the following claims and description.
Claims (8)
1. A correction method of giant panda urine hormone is characterized by comprising the following steps:
(a) collecting panda urine;
(b) measuring the original hormone concentration and the urine specific gravity of the panda urine;
(c) the original hormone concentration was corrected by the correction result of original hormone concentration/Lg urine specific gravity/500000 to obtain a correction result.
2. Correction method according to claim 1, characterized in that the hormone is an estrogen and/or a progestin.
3. The calibration method according to claim 1, wherein the method for measuring the specific gravity of urine is a densitometer method or a dry chemistry strip method.
4. The calibration method according to claim 3, wherein the hydrometer method determining the specific gravity of urine includes:
the urine hydrometer is checked and corrected, then the urine hydrometer of the panda urine is measured, and after the measurement is finished, the urine hydrometer is cleaned.
5. The correction method according to claim 4, wherein the verifying the correction comprises:
deionized water is added into a sample groove of the uropycnometer, and the deionized water in the sample groove is sucked out when the reading of the uropycnometer is fixed to be 1.000.
6. The calibration method according to claim 4, wherein the cleaning solution used in the cleaning is deionized water, and the reading of the uropycnometer is fixed to 1.000 after the deionized water is added.
7. The calibration method according to claim 1, wherein said original hormone concentration is determined by enzyme-linked immunosorbent assay.
8. The calibration method according to claim 1, wherein the panda urine collection method comprises:
cleaning and drying the ground of the animal house;
putting the pandas into a animal house, collecting urine by using an injector once the pandas discharge urine, filling the urine into a freezing pipe, and immediately putting the freezing pipe into a refrigerator for freezing or refrigerating.
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