CN109082364A - A kind of micro-structural device of physical method clasmatosis and its clasmatosis and processing method - Google Patents
A kind of micro-structural device of physical method clasmatosis and its clasmatosis and processing method Download PDFInfo
- Publication number
- CN109082364A CN109082364A CN201810888197.5A CN201810888197A CN109082364A CN 109082364 A CN109082364 A CN 109082364A CN 201810888197 A CN201810888197 A CN 201810888197A CN 109082364 A CN109082364 A CN 109082364A
- Authority
- CN
- China
- Prior art keywords
- cell
- pipe unit
- suspending liquid
- clasmatosis
- carrier gas
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/16—Microfluidic devices; Capillary tubes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
- C12M35/04—Mechanical means, e.g. sonic waves, stretching forces, pressure or shear stimuli
Landscapes
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Genetics & Genomics (AREA)
- General Engineering & Computer Science (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Cell Biology (AREA)
- Mechanical Engineering (AREA)
- Dispersion Chemistry (AREA)
- Clinical Laboratory Science (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The structure of the micro-structural device of physical method clasmatosis of the present invention are as follows: nitrogen input channel, cell suspending liquid input channel, broken chamber and crushing shell.Broken chamber and its connected runner are processed using organic polymer method of molding, the sealing-in of broken chamber is then realized with thermal bonding, other parts can realize connection with the method for welding.Clasmatosis system ruptures cell using the method for physical impacts, extracts target component in cell and carries out next step experiment.For cell suspending liquid with spray form by pipe outlet, the partial size of spraying granule is about 5 microns.High velocity carrier gas nitrogen flow rate is 200-300m/s, spray form particulate is sent into broken chamber, high-speed impact crushing shell is crushed.Then cell residue object is collected in broken chamber and is exported from outlet carries out further microfluidic control experiment.
Description
Technical field
The present invention relates to a kind of micro-structural device of physical method clasmatosis and its clasmatosis and processing method, belong to thin
Born of the same parents are crushed field.
Background technique
Micro-fluidic chip (microfluidic chip) is current micro-total analysis system (Miniaturized Total
Analysis Systems) development hot fields.Its target is the function entire laboratory, including sampling, dilution plus
Multiple links such as reagent, reaction, separation, detection are integrated on microchip, and can be used for multiple times, but are with cell (bacterium)
The micro-fluidic chip of operation object all the time without the feasible clasmatosis scheme of system.Common cell breaking technology can
It is divided into Mechanical Method and on-mechanical method, Mechanical Method includes:
High-pressure homogenization crush method (homogenization), pearl hits crush method (Skaking Bead), high-speed stirred pearl is ground for oscillation
Grind crush method (fine grinding), ultrasonic fragmentation (ultrasonication)；On-mechanical method includes: osmotic shock
Crush method (osmotic shock), freeze thawing crush method (freezing and thawing), the molten crush method (enzyme of enzyme
Lysis), chemically fragmenting method (chemical treatment), detergent crush method (detergents).
Method of cell disruption is applied to the generally existing Railway Project of micro-fluidic chip at present:
1, crushing system volume itself is excessive, cannot be combined with microfluidic system.
2, chemical impurity is introduced, contamination of cells fragment influences the subsequent operation of experiment.
3, the degree of crushing of cell can not be adjusted, and be likely to result in the overcrushing or broken inadequate of cell.
4, cataclasitlc structure is complicated, structure processing difficulties.
Summary of the invention
Technical problem: in order to overcome the shortcomings of above-mentioned existing cell breaking technology, the present invention provides one kind to be based on miniflow
The novel cell cataclasitlc structure of chip is controlled, it can effectively be crushed complete cell or bacterium, guarantee intracellular organelle
Integrality lay a good foundation for subsequent experimental on micro-fluidic chip.
Technical solution: in order to achieve the above technical purposes, the present invention adopts the following technical scheme:
A kind of micro-structural device of physical method clasmatosis, comprising:
Broken chamber, for collecting cell residue object, the top of broken chamber is equipped with the lid that can cover conjunction or opening；
Cell suspending liquid input channel, one end are connected to connection with the side cavity wall of the broken chamber, and the other end and cell suspend
The connection of liquid conveying equipment；
Crushing shell is arranged in crusher chamber room and is located at cell suspending liquid input channel exit, suspends on crushing shell towards cell
The one side of liquid input channel outlet end is equipped with puncture structure；
High velocity carrier gas input channel, one end are connected to connection with the cell suspending liquid input channel, and the other end is set with high pressure gas transmission
Standby connection, for by the cell suspending liquid in cell suspending liquid input channel in spray form and flow velocity not less than 200m/s's
Initial velocity hits the crushing shell；Gas transmission equipmen is pressed to be equipped with the regulating valve for adjusting high velocity carrier gas flow velocity；
Flushing liquor input port is opened in the side for being located at puncture structure on crushing shell on crusher chamber chamber wall, passes through pipeline and punching
Washing lotion conveying equipment connection, for broken chamber inner wall and crushing shell be rinsed；
Gas delivery port is set on lid, for discharging the indoor high pressure gas of crusher chamber；
Target liquid delivery outlet, connect with extraction module, for collecting Objective extraction liquid.
The cell suspending liquid input channel includes that the different cell suspending liquid of two diameters inputs pipe unit, is respectively
Diameter thinner first cell suspending liquid input pipe unit and thicker the second cell suspending liquid of diameter input pipe unit, wherein
One end of first cell suspending liquid input pipe unit is directly connected to setting, also, the first cell with the side cavity wall of broken chamber
The side side wall that suspension inputs the axis of pipe unit and the crushing shell is equipped with puncture structure is perpendicular；Second cell suspends
Liquid inputs the intersection setting between pipe unit and first cell suspending liquid input pipe unit at an angle, and first
The intersection of cell suspending liquid input pipe unit and the second cell suspending liquid input pipe unit has one to cross slot；
The high velocity carrier gas input channel includes the different high velocity carrier gas input channel of two diameters, is that diameter is thinner respectively
First high velocity carrier gas inputs pipe unit and thicker the second high velocity carrier gas of diameter inputs pipe unit, wherein the first high velocity carrier gas is defeated
The one end for entering pipe unit is connected with the slot that crosses, also, the first high velocity carrier gas input pipe unit and the first cell suspending liquid are defeated
Enter the coaxial setting of pipe unit, the first high velocity carrier gas inputs the other end and the input pipe unit connection of the second high velocity carrier gas of pipe unit.
The caliber of the first cell suspending liquid input pipe unit is 5 microns, and the second cell suspending liquid inputs pipe unit
Caliber is 15 microns.
The caliber of the first high velocity carrier gas input pipe unit is 3 microns, and the caliber that the second carrier gas inputs pipe unit is 10
Micron.
Puncture structure is pointed cone array structure on the crushing shell, and the distance between each pointed cone is 300nm.
A kind of method of cell disruption of the micro-structural device based on the physical method clasmatosis, including following step
It is rapid:
S1, high pressure gas transmission equipmen is opened, high pressure gas transmission equipmen passes through high velocity carrier gas input channel to cell suspending liquid input channel
Interior conveying high-pressure carrier gas, by adjusting the gas flow rate of valve regulation high pressure gas transmission equipmen to reaching requirement；
S2, cell suspending liquid conveying equipment is opened, cell suspending liquid is broken in the feeding of spray form under the carrying of high pressure carrier gas
Chamber, high-speed impact crushing shell, cell or bacterium are crushed；
After S3, clasmatosis, extraction module collects Objective extraction liquid by target liquid delivery outlet；
After S4, Objective extraction liquid are collected, cleaning solution is conveyed into crusher chamber room by washing lotion conveying equipment, by crusher chamber
The inner wall and crushing shell of room are rinsed.
The high velocity carrier gas is nitrogen.
The flow velocity of the high velocity carrier gas is 200-300m/s.
The micro-structural device of the physical method clasmatosis is applied in the Integrated manufacture of micro-fluidic chip.
A kind of processing method of such as micro-structural device of the physical method clasmatosis, is added using organic polymer method of molding
Work goes out broken chamber and coupled runner, the sealing-in of broken chamber is then realized using thermal bonding, on elastomeric stamp
Groove in fill up macromolecule prepolymer, be buckled on substrate, after solidification, remove mould, macromolecule material is printed on substrate
Expect the figure constituted, the substrate processed and the cover plate of same material cleaned after drying alignment is close to and lain in high temperature furnace,
One piece of polished graphite plate is respectively put in substrate and cover plate upper and lower, presses one piece of weight 0.5Kg again on graphite plate above not
Become rusty bloom, and bonding is heated in high temperature furnace, and bonding temperature is 1000 DEG C or more.
A kind of micro-structural device of physical method clasmatosis of the present invention and its beneficial effect of method of cell disruption are:
The present invention is crushed cell (bacterium) using physical method, and the broken cell of this method has the following characteristics that
First, clasmatosis occurs over just a flash hit with crushing shell, and clasmatosis is more uniform, can avoid cell stress repeatedly
Overcrushing phenomenon occurs；
2nd, clasmatosis degree can be controlled by electrodeless adjustment nebulizer gas pressure (flow velocity), avoid structural damage intracellular, be applicable in
In the recycling of organelle.
The micro-structural device of 3rd, physical method clasmatosis of the present invention is simple for structure, and design is easy to process, can quickly have
Effect ground, which is realized, is crushed cell, ensure that the progress of subsequent experimental on micro-fluidic chip, to the miniflow for promoting Highgrade integration
Control system has very profound significance.
Detailed description of the invention
Fig. 1 is the stereoscopic schematic diagram of the non-cap body of micro-structural device of physical method clasmatosis of the present invention；
Fig. 2 is that the micro-structural device of physical method clasmatosis of the present invention is stamped the stereoscopic schematic diagram of lid；
Fig. 3 is the structural schematic diagram of clasmatosis plate；
Fig. 4 is the cross-sectional view of the micro-structural device of physical method clasmatosis of the present invention；
Fig. 5 is the micro-structural device procedure of processing flow chart of physical method clasmatosis of the present invention；
Have in figure: 1, crushing shell；2, cell suspending liquid input channel；3, high velocity carrier gas input channel；4, it is crushed chamber；5, nitrogen
Gas and cell liquid river conjunction；6, cell suspending liquid delivery outlet；7, flushing liquor input port；8, target liquid delivery outlet；9, exhaust gas is discharged
Mouthful；10, gas delivery port.
Specific embodiment
With reference to the accompanying drawings of the specification and specific embodiment to the micro-structural device of physical method clasmatosis of the present invention and
The technical solution of its breaking method is described in further detail.
As shown in Figures 1 to 3, a kind of micro-structural device of physical method clasmatosis, comprising:
Broken chamber 4 occurs room for clasmatosis, collects cell residue object；
Cell suspending liquid input channel 2, one end are connected to connection with the side cavity wall of the broken chamber, and the other end and cell suspend
The connection of liquid conveying equipment；
Crushing shell 1 is arranged in broken chamber 4 and is located at cell suspending liquid input channel exit, outstanding towards cell on crushing shell 1
The one side of supernatant liquid input channel outlet end is equipped with puncture structure；
High velocity carrier gas input channel 3, one end are connected to connection, the other end and high pressure gas transmission with the cell suspending liquid input channel 2
Equipment connection, for by the cell suspending liquid in cell suspending liquid input channel in spray form and flow velocity be not less than 200m/s
Initial velocity hit the crushing shell.To realize spray form effect, cell suspending liquid delivery outlet 6 uses array of orifices structure, hole
Diameter be about 5 microns；Gas transmission equipmen is pressed to be equipped with the regulating valve for adjusting high velocity carrier gas flow velocity；
Flushing liquor input port 7 is opened in the side for being located at puncture structure on crushing shell on crusher chamber chamber wall, passes through pipeline and punching
Washing lotion conveying equipment connection, for broken chamber inner wall and crushing shell be rinsed；
Gas delivery port, for discharging the indoor high pressure gas of crusher chamber；
Target liquid delivery outlet 8, connect with extraction module, for collecting Objective extraction liquid.
Further, the cell suspending liquid input channel includes the different cell suspending liquid input pipe list of two diameters
Member is diameter thinner first cell suspending liquid input pipe unit and thicker the second cell suspending liquid input pipe list of diameter respectively
Member, wherein one end of the first cell suspending liquid input pipe unit is directly connected to setting with the side cavity wall of broken chamber, also,
The side side wall that first cell suspending liquid inputs the axis of pipe unit and the crushing shell is equipped with puncture structure is perpendicular；Second
Cell suspending liquid inputs the intersection setting between pipe unit and first cell suspending liquid input pipe unit at an angle, and
And have one to cross slot in the intersection that the first cell suspending liquid inputs pipe unit and the second cell suspending liquid input pipe unit；
The high velocity carrier gas input channel includes the different high velocity carrier gas input channel of two diameters, is that diameter is thinner respectively
First high velocity carrier gas inputs pipe unit and thicker the second high velocity carrier gas of diameter inputs pipe unit, wherein the first high velocity carrier gas is defeated
The one end for entering pipe unit is connected with the slot that crosses, also, the first high velocity carrier gas input pipe unit and the first cell suspending liquid are defeated
Enter the coaxial setting of pipe unit, the first high velocity carrier gas inputs the other end and the input pipe unit connection of the second high velocity carrier gas of pipe unit.
Embodiment 1
The caliber of first cell suspending liquid input pipe unit of the present invention is 5 microns, and the second cell suspending liquid inputs pipe unit
Caliber be 100 microns.Purpose be in order to by cell suspending liquid be dispersed as partial size be 5 microns particle and hit crushing shell.Institute
The caliber for stating the first high velocity carrier gas input pipe unit is 50 microns, and the caliber that the second carrier gas inputs pipe unit is 100 microns, purpose
It is the output flow velocity for improving nitrogen.It, can be by this structure process into micro-fluidic chip because the structural volume is small.
Embodiment 2
The caliber of first cell suspending liquid input pipe unit of the present invention is 5 microns, and the second cell suspending liquid inputs pipe unit
Caliber be 100 microns.Purpose be in order to by cell suspending liquid be dispersed as partial size be 5 microns particle and hit crushing shell.Institute
The caliber for stating the first high velocity carrier gas input pipe unit is 50 microns, and the caliber that the second carrier gas inputs pipe unit is 100 microns, purpose
It is the output flow velocity for improving nitrogen.Filter device is added in the output end of broken chamber, filter out DNA in cell residue object or
Then output liquid stream is entered in PCR instrument to replicate by person RNA, which can simplify experimental procedure, when saving experimental implementation
Between.
As the further preferred scheme of technical solution of the present invention, puncture structure is pointed cone array junctions on the crushing shell
Structure, the distance between each pointed cone are 300nm-10 μm.Guarantee that each cell (bacterium) for theoretically hitting crushing shell is equal
It can be broken.
The present invention further discloses a kind of clasmatosis of micro-structural device based on the physical method clasmatosis
Method, including the following steps:
S1, the gas delivery port for opening broken chamber, then open high pressure gas transmission equipmen, high pressure gas transmission equipmen passes through high velocity carrier gas
Input channel conveying high-pressure carrier gas into cell suspending liquid input channel, by the gas stream for adjusting valve regulation high pressure gas transmission equipmen
Speed is to reaching requirement；
S2, cell suspending liquid conveying equipment is opened, cell suspending liquid is broken in the feeding of spray form under the carrying of high pressure carrier gas
Chamber, high-speed impact crushing shell, cell or bacterium are crushed；
After S3, clasmatosis, extraction module collects Objective extraction liquid by target liquid delivery outlet；
After S4, Objective extraction liquid are collected, cleaning solution is conveyed into crusher chamber room by washing lotion conveying equipment, by crusher chamber
The inner wall and crushing shell of room are rinsed.
Further, the high velocity carrier gas is nitrogen.
Further, the flow velocity of the high velocity carrier gas is 200-300m/s.
Further, the micro-structural device of the physical method clasmatosis is applied in the Integrated manufacture of micro-fluidic chip.
As shown in figure 4, the present invention processes broken chamber and coupled runner using organic polymer method of molding,
Then the sealing-in of broken chamber is realized using thermal bonding.The material that crushing system uses is monocrystalline silicon.Specific processing technology is as follows:
Macromolecule prepolymer is filled up in groove on elastomeric stamp, is buckled on substrate, after solidification, mould is removed, on substrate
The figure for just having stamped high molecular material composition will be after the substrate that processed and the clean drying alignment abutting of the cover plate of same material
It lies in high temperature furnace, respectively puts one piece of polished graphite plate in substrate and cover plate upper and lower, pressed again on graphite plate above
The piece of stainless steel of one piece of weight 0.5Kg, heats bonding in high temperature furnace.Bonding temperature is 1000 DEG C or more.
Bacteria breaking system ruptures cell (bacterium) using the method for physical impacts, extracts mesh in cell (bacterium)
It marks ingredient and carries out next step experiment.For cell suspending liquid with spray form by pipe outlet, the partial size of spraying granule is about 5 micro-
Rice.Spray form particulate is sent into broken chamber by high velocity carrier gas --- nitrogen flow rate 200-300m/s, and high-speed impact is broken
Plate is crushed.Then cell residue object is collected in broken chamber and is exported from outlet carries out further microfluidic control experiment.
Fluid course diameter prepared by the present invention is micron level, and required precision is not high therefore method of molding can be used and added
Work, distance is 300 nanometers between the pointed cone of crushing shell, and design can be adjusted according to cell size.With existing clasmatosis side
Method is compared, and this method is simple for structure, is crushed uniform and complete, is facilitated processing and is carried out subsequent experimental to Related Component in cell,
It will be applied in the Integrated manufacture of micro-fluidic chip, realize automation, integration.
Claims (10)
1. a kind of micro-structural device of physical method clasmatosis characterized by comprising
Broken chamber, for collecting cell residue object, the top of broken chamber is equipped with the lid that can cover conjunction or opening；
Cell suspending liquid input channel, one end are connected to connection with the side cavity wall of the broken chamber, and the other end and cell suspend
The connection of liquid conveying equipment；
Crushing shell is arranged in crusher chamber room and is located at cell suspending liquid input channel exit, suspends on crushing shell towards cell
The one side of liquid input channel outlet end is equipped with puncture structure；
High velocity carrier gas input channel, one end are connected to connection with the cell suspending liquid input channel, and the other end is set with high pressure gas transmission
Standby connection, for by the cell suspending liquid in cell suspending liquid input channel in spray form and flow velocity not less than 200m/s's
Initial velocity hits the crushing shell；Gas transmission equipmen is pressed to be equipped with the regulating valve for adjusting high velocity carrier gas flow velocity；
Flushing liquor input port is opened in the side for being located at puncture structure on crushing shell on crusher chamber chamber wall, passes through pipeline and punching
Washing lotion conveying equipment connection, for broken chamber inner wall and crushing shell be rinsed；
Gas delivery port is set on lid, for discharging the indoor high pressure gas of crusher chamber；
Target liquid delivery outlet, connect with extraction module, for collecting Objective extraction liquid.
2. the micro-structural device of physical method clasmatosis according to claim 1, which is characterized in that the cell suspending liquid
Input channel includes that the different cell suspending liquid of two diameters inputs pipe unit, is that thinner first cell of diameter suspends respectively
Liquid inputs pipe unit and thicker the second cell suspending liquid of diameter inputs pipe unit, wherein the first cell suspending liquid input pipe list
One end of member is directly connected to setting, also, the axis of the first cell suspending liquid input pipe unit with the side cavity wall of broken chamber
The side side wall for being equipped with puncture structure with the crushing shell is perpendicular；Second cell suspending liquid inputs pipe unit and described first
Cell suspending liquid input pipe unit between at an angle intersection setting, and the first cell suspending liquid input pipe unit and
The intersection of second cell suspending liquid input pipe unit has one to cross slot；
The high velocity carrier gas input channel includes the different high velocity carrier gas input channel of two diameters, is that diameter is thinner respectively
First high velocity carrier gas inputs pipe unit and thicker the second high velocity carrier gas of diameter inputs pipe unit, wherein the first high velocity carrier gas is defeated
The one end for entering pipe unit is connected with the slot that crosses, also, the first high velocity carrier gas input pipe unit and the first cell suspending liquid are defeated
Enter the coaxial setting of pipe unit, the first high velocity carrier gas inputs the other end and the input pipe unit connection of the second high velocity carrier gas of pipe unit.
3. the micro-structural device of physical method clasmatosis according to claim 2, which is characterized in that first cell is outstanding
The caliber that supernatant liquid inputs pipe unit is 5 microns, and the caliber that the second cell suspending liquid inputs pipe unit is 15 microns.
4. the micro-structural device of physical method clasmatosis according to claim 2, which is characterized in that first high speed carries
The caliber that gas inputs pipe unit is 3 microns, and the caliber that the second carrier gas inputs pipe unit is 10 microns.
5. the micro-structural device of physical method clasmatosis according to claim 1, which is characterized in that pierced on the crushing shell
Broken structure is pointed cone array structure, and the distance between each pointed cone is 300nm.
6. a kind of method of cell disruption of the micro-structural device based on the physical method clasmatosis any in Claims 1 to 5,
It is characterised in that it includes following steps:
S1, high pressure gas transmission equipmen is opened, high pressure gas transmission equipmen passes through high velocity carrier gas input channel to cell suspending liquid input channel
Interior conveying high-pressure carrier gas, by adjusting the gas flow rate of valve regulation high pressure gas transmission equipmen to reaching requirement；
S2, cell suspending liquid conveying equipment is opened, cell suspending liquid is broken in the feeding of spray form under the carrying of high pressure carrier gas
Chamber, high-speed impact crushing shell, cell or bacterium are crushed；
After S3, clasmatosis, extraction module collects Objective extraction liquid by target liquid delivery outlet；
After S4, Objective extraction liquid are collected, cleaning solution is conveyed into crusher chamber room by washing lotion conveying equipment, by crusher chamber
The inner wall and crushing shell of room are rinsed.
7. the method for cell disruption of the micro-structural device of physical method clasmatosis according to claim 6, which is characterized in that
The high velocity carrier gas is nitrogen.
8. the method for cell disruption of the micro-structural device of physical method clasmatosis according to claim 6, which is characterized in that
The flow velocity of the high velocity carrier gas is 200-300m/s.
9. the method for cell disruption of the micro-structural device of physical method clasmatosis according to claim 6, which is characterized in that
The micro-structural device of the physical method clasmatosis is applied in the Integrated manufacture of micro-fluidic chip.
10. a kind of processing method of the micro-structural device of the physical method clasmatosis as described in any in Claims 1 to 5 uses
Organic polymer method of molding processes broken chamber and coupled runner, then realizes broken chamber using thermal bonding
Sealing-in, which is characterized in that fill up macromolecule prepolymer in the groove on elastomeric stamp, be buckled on substrate, after solidification, moved
Mould is removed, the figure of high molecular material composition is printed on substrate, the substrate processed and the cover plate of same material are cleaned and dried
Dry alignment is lain in high temperature furnace after being close to, and one piece of polished graphite plate is respectively put in substrate and cover plate upper and lower, above
The piece of stainless steel for pressing one piece of weight 0.5Kg on graphite plate again, heats bonding in high temperature furnace, and bonding temperature is 1000 DEG C or more.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810888197.5A CN109082364B (en) | 2018-08-01 | 2018-08-01 | A kind of micro-structural device of physical method clasmatosis and its clasmatosis and processing method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810888197.5A CN109082364B (en) | 2018-08-01 | 2018-08-01 | A kind of micro-structural device of physical method clasmatosis and its clasmatosis and processing method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN109082364A true CN109082364A (en) | 2018-12-25 |
| CN109082364B CN109082364B (en) | 2019-07-30 |
Family
ID=64834193
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810888197.5A Active CN109082364B (en) | 2018-08-01 | 2018-08-01 | A kind of micro-structural device of physical method clasmatosis and its clasmatosis and processing method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109082364B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2023247386A1 (en) * | 2022-06-20 | 2023-12-28 | LenioBio GmbH | Tuneable disruption of eukaryotic protoplast to release intact cellular organelles |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2552934Y (en) * | 2002-07-01 | 2003-05-28 | 苏奎荣 | Pressure circulating ultra-high pressure cell rupture device |
| CN1786148A (en) * | 2004-12-10 | 2006-06-14 | 中国科学院海洋研究所 | Wall breaking method of microalgae cell |
| CN105925472A (en) * | 2016-05-13 | 2016-09-07 | 聊城万合工业制造有限公司 | Industrial ultrahigh-pressure cell disruption method and cell disruption machine |
| CN107988061A (en) * | 2017-12-28 | 2018-05-04 | 广州聚能纳米生物科技股份有限公司 | A kind of cell breaking plant |
-
2018
- 2018-08-01 CN CN201810888197.5A patent/CN109082364B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2552934Y (en) * | 2002-07-01 | 2003-05-28 | 苏奎荣 | Pressure circulating ultra-high pressure cell rupture device |
| CN1786148A (en) * | 2004-12-10 | 2006-06-14 | 中国科学院海洋研究所 | Wall breaking method of microalgae cell |
| CN105925472A (en) * | 2016-05-13 | 2016-09-07 | 聊城万合工业制造有限公司 | Industrial ultrahigh-pressure cell disruption method and cell disruption machine |
| CN107988061A (en) * | 2017-12-28 | 2018-05-04 | 广州聚能纳米生物科技股份有限公司 | A kind of cell breaking plant |
Non-Patent Citations (1)
| Title |
|---|
| 陈建军: "基于MEMS技术的细胞破碎微流控芯片系统研究", 《中国博士学位论文全文数据库信息科技辑》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2023247386A1 (en) * | 2022-06-20 | 2023-12-28 | LenioBio GmbH | Tuneable disruption of eukaryotic protoplast to release intact cellular organelles |
Also Published As
| Publication number | Publication date |
|---|---|
| CN109082364B (en) | 2019-07-30 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US10369568B2 (en) | Cell separation using microchannel having patterned posts | |
| US9212977B2 (en) | Cell separation using microchannel having patterned posts | |
| US20100186524A1 (en) | Aerosol Collection and Microdroplet Delivery for Analysis | |
| CN107008517B (en) | Micro-fluidic chip and its droplet generating means | |
| CN102000645B (en) | Method for removing atomizer obstructions | |
| CN103244468B (en) | Injection type jet flow cavitation generator and cavitation cell breaking plant | |
| CN109082364B (en) | A kind of micro-structural device of physical method clasmatosis and its clasmatosis and processing method | |
| CN110605148A (en) | Micro-channel structure, micro-fluidic chip and quantitative heterogeneous reaction method | |
| Chen et al. | Spinning micropipette liquid emulsion generator for single cell whole genome amplification | |
| WO2008104916A2 (en) | A cell lysis and/or mixing device | |
| CN211190233U (en) | Micro-channel structure and micro-fluidic chip for quantitative heterogeneous reaction | |
| CN106179148A (en) | The microreactor of a kind of accurate amplification, preparation method and applications | |
| CN101264431B (en) | Water-soluble nano material supercritical carbon dioxide anti-solvent preparing device | |
| CN201473321U (en) | Polysilicon stick thermodynamic breaker | |
| JP4668751B2 (en) | Powder manufacturing method | |
| Wu et al. | Surface behaviors of droplet manipulation in microfluidics devices | |
| CN208465826U (en) | High-tower granulation equipment for chemical fertilizer production | |
| Chen et al. | Microfluidic biochip for blood cell lysis | |
| WO2023236760A1 (en) | Printing apparatus matched with microfluidic chip and printing method | |
| JP2013540439A5 (en) | ||
| CN104624402A (en) | Cyclone separator | |
| CN104087506B (en) | The unicellular reverse transcription PCR analytical equipment of a kind of high-throughput | |
| TWI435755B (en) | A transonic recovery system for elements | |
| WO2020222776A1 (en) | Cell lysis with a microbead and thermal resistor | |
| Afonicheva et al. | Magnetic beads-based nucleic acids extraction in microfluidic chip |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |