CN109055256A - One plant of Escherichia coli and its application in preparation microbial detergent - Google Patents
One plant of Escherichia coli and its application in preparation microbial detergent Download PDFInfo
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- CN109055256A CN109055256A CN201810779220.7A CN201810779220A CN109055256A CN 109055256 A CN109055256 A CN 109055256A CN 201810779220 A CN201810779220 A CN 201810779220A CN 109055256 A CN109055256 A CN 109055256A
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- escherichia coli
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/185—Escherichia
- C12R2001/19—Escherichia coli
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D7/00—Compositions of detergents based essentially on non-surface-active compounds
- C11D7/22—Organic compounds
- C11D7/40—Products in which the composition is not well defined
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Abstract
The invention discloses one plant of Escherichia coli and its applications in preparation microbial detergent.Escherichia coli (Escherichia coli) CF16, its deposit number are as follows: GDMCC 1.1478, the bacterium has ability that is relatively strong and quickly removing greasy dirt on tableware, therefore Escherichia coli (Escherichia coli) CF16 of the invention can be used to prepare microbial detergent.The bacterium can fall off the grease stain on a variety of tablewares and decompose to grease stain with fast and efficiently degradation capability, fermentation liquid, and secondary pollution does not occur.Escherichia coli (Escherichia coli) CF16 of the invention is the powerful bacterial strain that degreases in tableware microbial detergent.
Description
Technical field:
The invention belongs to microbe application fields, and in particular to one plant of Escherichia coli and its preparation microbial detergent in
Application.
Background technique:
Dish washing detergent is indispensable household supplies in people's daily life.With the continuous improvement of living standards and
Environmental consciousness gradually increases, and people increasingly pay attention to health safety issues, and the requirement to dish washing detergent is also increasingly stringenter.
Based on the angle of sustainable development, the chemical industry of 21st century must be turned by Industry restructuring to " environmental-friendly " direction
Become, cleaning product is no exception.Cleaning product other than meeting the performances such as strong detergency, safety, economy, should also conform to environmental protection,
Mild wait requires." prohibit phosphorus, limit phosphorus " as caused by sodium tripolyphosphate (STPP) " waters eutrophication " to move, for ecology
And the considerations of environment, developing becomes research hotspot for phosphorus reagent with excellent washing aid performance.Dish-washing machine has clean dirt, disappears
The hope that people are freed from simple, repeated labour of washing the dishes can be achieved in the multi-functionals such as poison and drying, its appearance
It hopes.The development for the special purpose detergent that cooperation automatic dish-washing machine uses just starts to walk in China, therefore exploitation is suitble to wash the dishes
The mating detergent of machine is very of practical meaning and market prospects.Starting point of the invention is exactly the research in microbial detergent
Under hot spot trend, under the background lacked for the microbial resources of detergent, from the angle of Screening germplasm, efficient, side is provided
Just microorganism resource is that basic resource is contributed in the popularization of microbial detergent product.
Summary of the invention:
The object of the present invention is to provide a kind of Escherichia coli (Escherichia coli) CF16, the bacterial strain can efficiently,
Quickly, the grease stain on tableware is safely removed.
The first purpose of the invention is to provide one plant of isolated Escherichia coli from human excrement and urine
(Escherichia coli) CF16, the bacterium were preserved in Guangdong Province's Culture Collection on 07 13rd, 2018
(GDMCC), address: 5 building, the building of compound the 59th of Xianlie Middle Road, Guangzhou City 100, deposit number are as follows: GDMCC 1.1478.
The present inventor is experiments prove that Escherichia coli (Escherichia coli) CF16 has relatively strong and quickly removes
The ability of greasy dirt on tableware.
Therefore, a second object of the present invention is to provide the Escherichia coli (Escherichia coli) CF16 to go
Application in degreasing.
Third object of the present invention is to provide the Escherichia coli (Escherichia coli) CF16 prepare it is micro-
Application in biocleaner.
Fourth object of the present invention is to provide a kind of microbial detergent, with the Escherichia coli (Escherichia
Coli) fermentation liquid of CF16 is as active constituent.
It is preferred that the fermentation liquid is that Escherichia coli (Escherichia coli) CF16 is inoculated into fluid nutrient medium
Anaerobic fermentation obtains bacteria suspension, and fermentation liquid obtained from supernatant is collected after centrifugation.
Present invention Escherichia coli isolated from human excrement and urine (Escherichia coli) CF16, the bacterium is to grease stain
With fast and efficiently degradation capability, fermentation liquid can fall off the grease stain on a variety of tablewares and decompose, and secondary dirt does not occur
Dye.Escherichia coli (Escherichia coli) CF16 of the invention is that degreasing is powerful in tableware microbial detergent
Bacterial strain.
It is micro- that Escherichia coli (Escherichia coli) CF16 of the invention on 07 13rd, 2018 was preserved in Guangdong Province
Biological inoculum collection (GDMCC), address: 5 building, the building of compound the 59th of Xianlie Middle Road, Guangzhou City 100, deposit number are as follows:
GDMCC 1.1478。
Specific embodiment:
The following examples are further illustrations of the invention, rather than limiting the invention.
Embodiment 1: the separation and identification of Escherichia coli (Escherichia coli) CF16 bacterial strain
The separation of Escherichia coli 1. (Escherichia coli) CF16 bacterial strain
Screening and culturing medium: TSA culture medium: trypticase 15g, soy peptone 5g, sodium chloride 5g, agar 15g, distilled water
1000mL adjusts pH 7.1~7.5；Acetic acid culture medium: glucose 10g, yeast extract 15g, KH2PO40.5g, MgSO4·
7H2O3g, dehydrated alcohol 5.6%, distilled water 1000mL adjust pH 7.0~7.2.
Escherichia coli (Escherichia coli) CF16 bacterial strain of the invention is the Check-up crowd from Guangzhou Nanfang Hospital
Excrement in separation, purifying and obtain.Its isolation and purification method is as follows:
Take 1g fecal sample closed enrichment culture in the screening and culturing medium of 100mL.Pass through different time in enrichment process
Check the turbidity of fermentation liquid.Enrichment culture is cultivated 1 day at 37 DEG C, after having observed apparent muddiness, is transferred to and is newly matched
Continue culture 1 day in the screening and culturing medium set under the same terms.Then Mixed Microbes are taken in the culture solution of upper step enrichment culture
Group is applied in the solid screening and culturing medium containing agar and is cultivated in 37 DEG C of anaerobism work, therefrom the bacterium of picking fast-growth
Capable purifying and oily waste degradation test are dropped into, one plant of bacterial strain to grease stain with fast and efficiently degradation capability is finally obtained
CF16。
2. the physical and chemical parameter of bacterial strain CF16 is as follows:
1, strain characteristic
A. using conventional Bacterial Physiological biochemical identification method and electron microscope observation, bacterial strain CF16 is gram-negative
Property, rod-shaped, oxidase negative cannot move.
B. after cultivating 20h on TSA culture medium flat plate, bacterium colony surface is in ecru, round, no protrusion.
2, the main physicochemical property of Escherichia coli (Escherichia coli) CF16:
The 16S rRNA gene sequencing of Escherichia coli (Escherichia coli) CF16, BLAST compare discovery, the present invention
Escherichia coli (Escherichia coli) CF1616S rRNA gene order and Escherichia coli have mode bacterium
Strain similitude with higher.It is wherein 99% with the sequence similarity of Escherichia coli DSM 30083T.
In summary physio-biochemical characteristics, 16S rRNA gene order is as a result, bacterial strain CF16 of the present invention should belong to
Escherichia coli is named as Escherichia coli (Escherichia coli) CF16, was preserved on 07 13rd, 2018
Guangdong Province's Culture Collection (GDMCC), address: 5 building, the building of compound the 59th of Xianlie Middle Road, Guangzhou City 100, preservation
Number are as follows: GDMCC 1.1478.
Embodiment 2:
1. the preparation of fermentation liquid:
Escherichia coli (Escherichia coli) CF16 is inoculated into 1L TSA fluid nutrient medium (TAS fluid nutrient medium
Formula are as follows: trypticase 15g, soy peptone 5g, sodium chloride 5g, agar 15g, distilled water 1000mL adjust pH 7.1~7.5；Vinegar
Sour culture medium: glucose 10g, yeast extract 15g, KH2PO40.5g, MgSO4·7H2O 3g, dehydrated alcohol 5.6%, distillation
Water 1000mL) in, it is subsequently placed in static gas wave refrigerator in 37 DEG C of anaerobic box, culture obtained bacteria suspension after 10 hours, with physiological saline tune
Whole bacteria suspension concentration is 0.2 to OD590, and then 8000r/min is centrifuged, and is collected supernatant, is obtained fermentation liquid.
2. oily waste degradation is tested:
The plate dehydrated alcohol wiped clean that needs are cleaned dries weighing record M1, vegetable oil is poured into tableware
10s is impregnated, 30min is dried, the oil that do not inhale on tableware is wiped with filter paper, and weighing is recorded as M2 again, by the microbial fermentation of preparation
Liquid is diluted with water to the detergent solution that volume fraction is 1%, and (detergent solution that volume fraction is 1% is sent out according to every milliliter
Zymotic fluid is added what 99 milliliters of water diluted).It is molten that the detergent that 2500mL volume fraction is 1% is added in the plastic barrel of each 5L
Liquid (i.e. microbial detergent), water temperature are controlled at 35 ± 2 DEG C, and the tableware for being coated with grease stain is immersed in the washing of volume fraction 1%
30s is stirred in agent solution, and then tableware is placed on in 60 DEG C of drying box dry 6h, weigh after room temperature is cooling, be recorded as M3.Weight
It is 3 times multiple.Oil cut rate is gone in calculating.3 times oil removing result is shown in Table 1~3 respectively.
The oil removing result of 1 microbial detergent of table
The oil removing result of 2 microbial detergent of table
The oil removing result of 3 microbial detergent of table
Escherichia coli of the invention (Escherichia coli) CF16 has relatively strong and quick it can be seen from table 1~3
Remove the ability of greasy dirt on tableware.
Claims (5)
- Escherichia coli 1. (Escherichia coli) CF16, deposit number is GDMCC 1.1478.
- 2. application of Escherichia coli (Escherichia coli) CF16 in degreasing described in claim 1.
- 3. Escherichia coli (Escherichia coli) CF16 described in claim 1 answering in preparation microbial detergent With.
- 4. a kind of microbial detergent, which is characterized in that with Escherichia coli described in claim 1 (Escherichia coli) The fermentation liquid of CF16 is as active constituent.
- 5. microbial detergent according to claim 4, which is characterized in that the fermentation liquid is by Escherichia coli (Escherichia coli) CF16 is inoculated into anaerobic fermentation in fluid nutrient medium, obtains bacteria suspension, supernatant is collected after centrifugation Obtained from fermentation liquid.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810779220.7A CN109055256B (en) | 2018-07-16 | 2018-07-16 | Escherichia coli and application thereof in preparation of microbial detergent |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201810779220.7A CN109055256B (en) | 2018-07-16 | 2018-07-16 | Escherichia coli and application thereof in preparation of microbial detergent |
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| CN109055256A true CN109055256A (en) | 2018-12-21 |
| CN109055256B CN109055256B (en) | 2021-12-07 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111254169A (en) * | 2019-12-30 | 2020-06-09 | 广州舒国生物科技有限公司 | Application of escherichia coli CF16 in glutamic acid or sodium glutamate production |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103205378B (en) * | 2013-03-25 | 2014-04-23 | 天津科技大学 | Oil-degradation oil-and-salt tolerance bacterial strain, and screening method and application thereof |
-
2018
- 2018-07-16 CN CN201810779220.7A patent/CN109055256B/en active Active
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103205378B (en) * | 2013-03-25 | 2014-04-23 | 天津科技大学 | Oil-degradation oil-and-salt tolerance bacterial strain, and screening method and application thereof |
Non-Patent Citations (2)
| Title |
|---|
| 张英等: "影响废水处理中油脂生物降解的因素", 《广州环境科学》 * |
| 陈忠喜等: "除油生物表面活性剂产生茵的分离及其特性", 《哈尔滨工业大学学报》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111254169A (en) * | 2019-12-30 | 2020-06-09 | 广州舒国生物科技有限公司 | Application of escherichia coli CF16 in glutamic acid or sodium glutamate production |
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| CN109055256B (en) | 2021-12-07 |
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