CN109046026A - A kind of solution filtering device - Google Patents
A kind of solution filtering device Download PDFInfo
- Publication number
- CN109046026A CN109046026A CN201811068886.8A CN201811068886A CN109046026A CN 109046026 A CN109046026 A CN 109046026A CN 201811068886 A CN201811068886 A CN 201811068886A CN 109046026 A CN109046026 A CN 109046026A
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- CN
- China
- Prior art keywords
- filter device
- monomer
- aggregating apparatus
- filtrate
- filter
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
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- 238000001914 filtration Methods 0.000 title claims abstract description 39
- 239000000178 monomer Substances 0.000 claims abstract description 49
- 230000004931 aggregating effect Effects 0.000 claims abstract description 41
- 239000000706 filtrate Substances 0.000 claims abstract description 35
- 239000012528 membrane Substances 0.000 claims abstract description 22
- 238000005119 centrifugation Methods 0.000 claims description 5
- 239000008280 blood Substances 0.000 description 9
- 210000004369 blood Anatomy 0.000 description 9
- 238000002474 experimental method Methods 0.000 description 7
- 239000007788 liquid Substances 0.000 description 5
- 239000012535 impurity Substances 0.000 description 4
- 238000000034 method Methods 0.000 description 4
- 238000012360 testing method Methods 0.000 description 4
- 238000012864 cross contamination Methods 0.000 description 3
- 201000010099 disease Diseases 0.000 description 3
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 3
- 241000894006 Bacteria Species 0.000 description 2
- 239000012491 analyte Substances 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 238000009434 installation Methods 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 241001391944 Commicarpus scandens Species 0.000 description 1
- 239000000427 antigen Substances 0.000 description 1
- 102000036639 antigens Human genes 0.000 description 1
- 108091007433 antigens Proteins 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000004090 dissolution Methods 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 229920002521 macromolecule Polymers 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000001471 micro-filtration Methods 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 150000007523 nucleic acids Chemical class 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D61/00—Processes of separation using semi-permeable membranes, e.g. dialysis, osmosis or ultrafiltration; Apparatus, accessories or auxiliary operations specially adapted therefor
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5021—Test tubes specially adapted for centrifugation purposes
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D2311/00—Details relating to membrane separation process operations and control
- B01D2311/26—Further operations combined with membrane separation processes
- B01D2311/2649—Filtration
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D2317/00—Membrane module arrangements within a plant or an apparatus
- B01D2317/04—Elements in parallel
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D2317/00—Membrane module arrangements within a plant or an apparatus
- B01D2317/06—Use of membrane modules of the same kind
Landscapes
- Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Water Supply & Treatment (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Hematology (AREA)
- Clinical Laboratory Science (AREA)
- Sampling And Sample Adjustment (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses a kind of solution filtering devices comprising round cuvette, filter membrane and filtrate aggregating apparatus, the filtrate aggregating apparatus are fixed on round cuvette lower part, and the filter membrane is set between round cuvette and filtrate aggregating apparatus;Round cuvette, filter membrane and the filtrate aggregating apparatus collectively constitutes monomer filter device, and the monomer filter device is fixed on the fixed collection device of monomer;The solution filtering device can not only improve working efficiency, reduce the risk that sample is obscured or polluted, and applied widely, have the advantages that high throughput.
Description
Technical field
The present invention relates to filtration arts, and in particular to a kind of solution filtering device.
Background technique
Filter paper acquisition Blood piece is widely applied in clinical examination at this stage, the especially examination of newborn infant diseases, and method is
Blood sample is acquired using special filter paper, then uses instrument to cut the satisfactory blood cake of diameter as experiment sample according to experimental method
This, is put into dissolution in the microwell plate for experiment and elutes blood sample, carry out content detection to target analytes in Blood piece, thus
Aided disease judgement.After the completion of analyte is extracted, if being caused in various degree containing sector-meeting to different detection projects in microwell plate
Influence, some projects are to cause the failure of an experiment, can not obtain testing result;Some projects are to cause cross contamination or test,
Influence the accuracy of testing result;Also it will cause equipment faults for some projects, influence working efficiency.Therefore it is analyzed in blood cake
Object must remove Dried blood spots and impurity in reagent after extracting.
Current main way is that the solution in former experiment microwell plate is transferred to other one piece of sky manually using liquid-transfering gun
In microwell plate, there are corresponding shortcomings for the above way.All liquid relief movements are manually completed by experimenter, workload
Greatly, time-consuming, and the lesser impurity of particles in solution equally exists the possibility for being transferred to new microwell plate, influences subsequent behaviour
Make, while being likely to occur maloperation in operation, obscures sample position, and generate the cross contamination of sample, influence reality
Test the accuracy of result.
It is more and more with filter with many new instruments such as tandem mass spectrometer gradually popularizing in the examination of newborn infant diseases
The dry blood cake of paper is introduced to the market as the project of experiment sample, and the liquid after extracting to blood cake analyte carries out impurity removal and also requires
Higher and higher, existing liquid transfer method is gradually difficult to meet its requirement.There is also many microfiltrations in the market simultaneously
Device, such as: the filter core of filtration protein, the filter core of bacterium, purifying antigen antibody centrifuge tube.But above-mentioned apparatus none be not single
A sample filter device cannot achieve the multiple samples of disposable filtering.
Summary of the invention
The technical problem to be solved in the present invention is to provide a kind of solution filtering devices, can not only improve working efficiency, drop
The risk that low sample is obscured or polluted, and it is applied widely, have the advantages that high throughput.
Above-mentioned technical problem to be solved by this invention is achieved by the following technical programs:
A kind of solution filtering device comprising round cuvette, filter membrane and filtrate aggregating apparatus, the filtrate aggregating apparatus are fixed
In round cuvette lower part, the filter membrane is set between round cuvette and filtrate aggregating apparatus;The round cuvette, filter membrane and
Filtrate aggregating apparatus collectively constitutes monomer filter device, and the monomer filter device is fixed on the fixed collection device of monomer.
Preferably, the round cuvette is the frustum cone structure put upside down, and internal is cavity, and open-minded up and down, the filter membrane is
Circular configuration, size match with round cuvette lower end area, and the filtrate aggregating apparatus is the frustum cone structure put upside down,
It is cavity that it is internal, and upper end is open-minded;Filtrate aggregating apparatus upper inner diameter is matched with round cuvette lower outer diameter, and filtrate collects dress
Set and be fixed on round cuvette lower part, filter membrane by filter aggregating apparatus be fixed on round cuvette lower end, i.e., filtering aggregating apparatus with
Among round cuvette.
It is further preferred that the lower end of the filtering aggregating apparatus has the collector groove of back taper, collector groove lower end is
Through-hole.
Preferably, the fixed collection device of the monomer is centrifuge tube, and the centrifuge tube includes: centrifuge tube lid, centrifuge tube
Main body and connection strap, the centrifuge tube main body generally pyramidal structure, the upper end has the opening to match with centrifuge tube lid, internal
For cavity structure, centrifuge tube main body is divided into two sections;Upper section cross section is greater than lower section cross section, and upper intersegmental part places monomer mistake
Device is filtered, lower section blocks the lower end of monomer filter device just;There is protrusion platform, the protrusion among centrifuge tube lid lower end
The upper-end inner diameter of platform outer diameter and monomer filter device circle cuvette matches, and monomer filter device is fixed on centrifugation lid lower end;It is described
Connection strap both ends are respectively fixedly connected in centrifuge tube lid and centrifuge tube main body.
Preferably, adjacent monomer filter device is fixedly connected on one by connection structure by the monomer filter device
It rises, forms filter device group, the filter device group is made of more than one monomer filter device, and filter device group is fixed on
Filter device group fixes collection device.
It is further preferred that the connection structure includes: adhesion article and adhesion piece;The adhesion article both ends are separately connected
In the outer upper end of adjacent two round cuvettes;Adhesion piece both ends are connected to two neighboring filtering aggregating apparatus
Outer upper end, more than one monomer filter device are connected in a row by adhesion article and adhesion piece, collectively constitute filter device group.
It is further preferred that the viscous latticing is preferably two, their flush with each other, the adhesion article and circle are small
Cup connecting place is provided with cutting line, and the adhesion piece and filtrate aggregating apparatus connecting place are provided with cutting line.
It is further preferred that the round cuvette of the outermost two monomer filter devices of the filter device group is in the outer part
One end top have protrusion rectangle side handle.
It is further preferred that the fixed collection device of the filter device group is multi-well microplate, the multi-well microplate
On be evenly distributed with placed hole, each monomer filter device of filter device group corresponds the placed hole on multi-well microplate,
Filter device group is mounted on multi-well microplate.
It is further preferred that the multi-well microplate is preferably 96 hole microwell plates.
The utility model has the advantages that after using structure of the present invention, since solution filtering device is using round cuvette, filter membrane and filter
The filter device that liquid aggregating apparatus collectively constitutes, and monomer filter device or filter device group, there is corresponding centrifuge tube or more
Hole microwell plate fixes collection device, and fixed collection device has the one-to-one hole for being used to collect filtrate, realizes filtering, collects,
The repertoire of transfer can substantially reduce the labor intensity of operator relative to existing manual branch mode, reduce operation
Time, sample position is obscured caused by reducing because of artificial maloperation, the cross contamination of sample, and can filter out lesser
Impurity improves the accuracy and working efficiency of experimental result;Simultaneously because filter membrane is to be fixed on circle by filtrate aggregating apparatus
Under shape cuvette, and filtrate aggregating apparatus and round cuvette are detachable structure, keep the replacement of filter membrane simple and convenient, and can lead to
It crosses with the filter membrane of different pore size and filters the filtering of such as serum, bacterium, protein, nucleic acid macromolecular substances or even pearl, it is real
It is very wide with range;And filter device can be placed on multi-well microplate and be filtered, and disposably may filter that multiple samples, have
High-throughput advantage;Adhesion article and round cuvette connecting place are provided with cutting line, and adhesion piece and filtrate aggregating apparatus connecting place are set
It is equipped with cutting line, so that adhesion article and adhesion piece is easy to tear open and is broken, the filter device of suitable number can be selected according to demand to be filtered,
Filtrate aggregating apparatus lower end has the collector groove of back taper, makes what filtrate more concentrated to pool together, facilitates collection;And it filters
The monomer aggregating apparatus for collecting the outermost two sides of group is provided with side handle, and the installation facilitated in experimentation picks and places, transfer.
Detailed description of the invention
Fig. 1 is the top view that filter device group is placed on more microwell plates.
Fig. 2 is the exploded view of filter device group.
Fig. 3 is the top view of filter device group.
Fig. 4 is monomer filter apparatus configuration figure.
Fig. 5 is the exploded view of monomer filter device.
The structure chart of Fig. 6 centrifuge tube.
Fig. 7 is the installation cooperation figure of monomer filter device and centrifuge tube.
Specific embodiment
The present invention is described in further detail in the following with reference to the drawings and specific embodiments, but embodiment to the present invention not
It limits in any form.
Embodiment 1
A kind of solution filtering device as shown in figs. 1-7 comprising round cuvette (4), filter membrane (5) and filtrate aggregating apparatus (6),
Round cuvette (4), filter membrane (5) and the filtrate aggregating apparatus collectively constitutes monomer filter device (3), the circle cuvette
(4) be the frustum cone structure put upside down, internal is cavity, open-minded up and down, and the filter membrane (5) is circular configuration, size with
Round cuvette lower end area matches, and the filtrate aggregating apparatus (6) is the frustum cone structure put upside down, and internal is cavity, upper end
It is open-minded;Filtrate aggregating apparatus (6) upper inner diameter is matched with round cuvette (4) lower outer diameter, and filtrate aggregating apparatus (6) is fixed on
Round cuvette (4) lower part, filter membrane (5) are fixed on round cuvette (4) lower end by filtering aggregating apparatus (6), i.e. filtering collects dress
It sets (6) and round cuvette (4) is intermediate, the lower end of the filtering aggregating apparatus (6) has the collector groove (61) of back taper, collects
Slot (61) lower end is through-hole.The monomer filter device (3) is fixed on centrifuge tube (7), and the centrifuge tube (7) includes: centrifugation
Pipe lid (71), centrifuge tube main body (73) and connection strap (72), the centrifuge tube main body (73) generally pyramidal structure, the upper end tool
There is the opening to match with centrifuge tube lid (71), inside is cavity structure, and centrifuge tube main body (73) is divided into two sections;Upper section is transversal
Area is greater than lower section cross-sectional area, and upper intersegmental part places monomer filter device (3), and lower section blocks monomer filter device (3) just
Lower end, for collecting filtrate;There are protrusion platform (711) among centrifuge tube lid (71) lower end, the protrusion platform outer diameter with
The upper-end inner diameter of monomer filter device (3) circle cuvette (4) matches, and monomer filter device (3) is fixed on centrifugation lid (71) lower end;
Connection strap (72) both ends are respectively fixedly connected in centrifuge tube lid (71) and centrifuge tube main body (73).The monomer filters dress
It sets (3) and is fixedly connected on adjacent monomer filter device (3) together by connection structure, form filter device group (2), filtering
Device group (2) is made of more than one monomer filter device (3), and the connection structure includes: adhesion article (41) and adhesion
Piece (62);The adhesion article (41) has two, their flush with each other, and adhesion article (41) both ends are connected to two adjacent circles
The outer upper end of shape cuvette;Adhesion piece (62) both ends are connected on the outside of two neighboring filtering aggregating apparatus (6)
End, more than one monomer filter device (3) is even in a row by adhesion article (41) and adhesion piece (62), collectively constitutes filtering dress
Set group;The adhesion article (41) and round cuvette (4) connecting place are provided with cutting line, and the adhesion piece (62) and filtrate are converged
Acquisition means (6) connecting place is provided with cutting line, make adhesion article (41) and adhesion piece (62) be easy to break it is disconnected.The filter device group
(2) one end top of the round cuvette (4) of outermost two monomer filter devices (3) in the outer part has the rectangle side of protrusion
Handle (42).The filter device group (2) is fixed on multi-well microplate (1), and the multi-well microplate (1) is 96 hole micropores
Plate is rectangular parallelepiped structure, and uniform array has 8 × 12 placed holes on multi-well microplate (1), and multi-well microplate (1) places 12
Group filter device group (2), every group of filter device group (2) have 8 monomer filter devices (3), each monomer of filter device group (2)
Filter device (3) corresponds the placed hole on multi-well microplate (1);Multi-well microplate (1) upper end side blank space is each
The corresponding position of row indicates (11) with the sequence of A-H.
In use, selecting suitable filter membrane (5) according to material type to be filtered, according to the quantity of filtered fluid, it is suitable to select
Filter device quantity, liquid to be filtered is transferred on filter device, filter device is fixed on corresponding centrifuge tube (7) or more
Hole microwell plate (1) fixes collection device, is placed in centrifuge, selects suitable parameter of noncentricity to carry out centrifugal filtration, takes after centrifugation
Out, fixed collection device can obtain the corresponding filtered filtrate of filter device, gained filtrate can be carried out subsequent experiment and grasped
Make.
The foregoing is only a preferred embodiment of the present invention, but scope of protection of the present invention is not limited thereto,
Anyone skilled in the art in the technical scope disclosed by the present invention, according to the technique and scheme of the present invention and structure
Think of is subject to equivalent substitution or change, should be covered by the protection scope of the present invention.
Claims (10)
1. a kind of solution filtering device comprising round cuvette (4), filter membrane (5) and filtrate aggregating apparatus (6), which is characterized in that
The filtrate aggregating apparatus (6) is fixed on round cuvette (4) lower part, and the filter membrane (5) is set to round cuvette (4) and filtrate
Between aggregating apparatus (6);Round cuvette (4), filter membrane (5) and the filtrate aggregating apparatus collectively constitutes monomer filter device
(3), the monomer filter device (3) is fixed on the fixed collection device of monomer.
2. solution filtering device according to claim 1, which is characterized in that the circle cuvette (4) is the rotary table put upside down
Structure, internal is cavity, open-minded up and down, and the filter membrane (5) is circular configuration, size and round cuvette lower end area
Match, the filtrate aggregating apparatus (6) is the frustum cone structure put upside down, and internal is cavity, and upper end is open-minded;Filtrate aggregating apparatus
(6) upper inner diameter is matched with round cuvette (4) lower outer diameter, and filtrate aggregating apparatus (6) is fixed on round cuvette (4) lower part,
Filter membrane (5) is fixed on round cuvette (4) lower end by filtering aggregating apparatus (6), i.e. filtering aggregating apparatus (6) and round cuvette
(4) intermediate.
3. solution filtering device according to claim 2, which is characterized in that the lower end of the filtering aggregating apparatus (6)
Collector groove (61) with back taper, collector groove (61) lower end are through-hole.
4. solution filtering device according to claim 1, which is characterized in that the fixed collection device of the monomer is centrifugation
It manages (7), the centrifuge tube (7) includes: centrifuge tube lid (71), centrifuge tube main body (73) and connection strap (72), the centrifuge tube master
Body (73) generally pyramidal structure, the upper end have the opening to match with centrifuge tube lid (71), and inside is cavity structure, centrifuge tube
Main body (73) is divided into two sections;Upper section cross section is greater than lower section cross section, and upper intersegmental part places monomer filter device (3), under
Section blocks the lower end of monomer filter device (3) just;There are protrusion platform (711) among centrifuge tube lid (71) lower end, it is described
The upper-end inner diameter of the round cuvette (4) in protrusion platform outer diameter and monomer filter device (3) matches, monomer filter device (3) be fixed on from
Heart lid (71) lower end;Connection strap (72) both ends are respectively fixedly connected in centrifuge tube lid (71) and centrifuge tube main body (73).
5. solution filtering device according to claim 1, which is characterized in that the monomer filter device (3) passes through company
Adjacent monomer filter device (3) is fixedly connected on together by binding structure, is formed filter device group (2), the filter device group
(2) it is made of more than one monomer filter device (3), filter device group (2) is fixed on the fixed collection device of filter device group.
6. solution filtering device according to claim 5, which is characterized in that the connection structure includes: adhesion article
(41) and adhesion piece (62);Adhesion article (41) both ends are connected to the outer upper end of the round cuvette (4) of adjacent two;It is described
Adhesion piece (62) both ends are connected to the outer upper end of two neighboring filtering aggregating apparatus (6), more than one monomer filtering
Device (3) is even in a row by adhesion article (41) and adhesion piece (62), collectively constitutes filter device group.
7. solution filtering device according to claim 6, which is characterized in that the adhesion article (41) is preferably two,
Their flush with each other, the adhesion article (41) and round cuvette (4) connecting place are provided with cutting line, the adhesion piece (62)
Filtrate aggregating apparatus (6) connecting place is provided with cutting line.
8. solution filtering device according to claim 5, which is characterized in that the filter device group (2) is outermost
One end top of the round cuvette (4) of two monomer filter devices (3) in the outer part has the rectangle side handle (42) of protrusion.
9. solution filtering device according to claim 5, which is characterized in that the fixed collection device of the filter device group
For multi-well microplate (1), placed hole, each monomer mistake of filter device group (2) are evenly distributed on the multi-well microplate (1)
The placed hole on device (3) one-to-one correspondence multi-well microplate (1) is filtered, filter device group (2) is mounted on multi-well microplate (1)
On.
10. solution filtering device according to claim 9, which is characterized in that the multi-well microplate (1) is preferably 96
Hole microwell plate.
Priority Applications (1)
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CN201811068886.8A CN109046026A (en) | 2018-09-13 | 2018-09-13 | A kind of solution filtering device |
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CN201811068886.8A CN109046026A (en) | 2018-09-13 | 2018-09-13 | A kind of solution filtering device |
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Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN112067412A (en) * | 2020-10-09 | 2020-12-11 | 嘉兴晶铸生物科技有限公司 | Multi-well plate filtration device and method |
CN113751085A (en) * | 2020-06-05 | 2021-12-07 | 帕尔公司 | Porous devices and methods of use |
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CN205874413U (en) * | 2016-08-18 | 2017-01-11 | 李忠杰 | Trace DNA is extraction element in batches |
CN106591107A (en) * | 2017-01-12 | 2017-04-26 | 武汉菲思特生物科技有限公司 | Sample adding device for pyrosequencing |
CN209237726U (en) * | 2018-09-13 | 2019-08-13 | 广州俊通生物科技有限公司 | A kind of solution filtering device |
-
2018
- 2018-09-13 CN CN201811068886.8A patent/CN109046026A/en active Pending
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN205874413U (en) * | 2016-08-18 | 2017-01-11 | 李忠杰 | Trace DNA is extraction element in batches |
CN106591107A (en) * | 2017-01-12 | 2017-04-26 | 武汉菲思特生物科技有限公司 | Sample adding device for pyrosequencing |
CN209237726U (en) * | 2018-09-13 | 2019-08-13 | 广州俊通生物科技有限公司 | A kind of solution filtering device |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN113751085A (en) * | 2020-06-05 | 2021-12-07 | 帕尔公司 | Porous devices and methods of use |
US11504716B2 (en) | 2020-06-05 | 2022-11-22 | Pall Corporation | Multiwell device and method of use |
CN112067412A (en) * | 2020-10-09 | 2020-12-11 | 嘉兴晶铸生物科技有限公司 | Multi-well plate filtration device and method |
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