CN210775526U - Be used for a plurality of sample preprocessing devices of continuous flow analysis - Google Patents
Be used for a plurality of sample preprocessing devices of continuous flow analysis Download PDFInfo
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- CN210775526U CN210775526U CN201921170471.1U CN201921170471U CN210775526U CN 210775526 U CN210775526 U CN 210775526U CN 201921170471 U CN201921170471 U CN 201921170471U CN 210775526 U CN210775526 U CN 210775526U
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Abstract
The utility model discloses a be used for a plurality of sample pretreatment device of continuous flow analysis, including following part: a support (2); an extraction tube (a); an extraction pipe rack (3); a filtering pipe (b); a filter pipe frame (4); a lifting handle (5). Use the utility model discloses a device makes sample analysis efficiency can improve several times.
Description
Technical Field
The utility model belongs to tobacco chemistry research field, concretely relates to be used for a plurality of sample pretreatment devices of continuous flow analysis.
Background
With the advance of the industrial breeding scheme of the tobacco genome plan major special item based on the genome editing technology in the tobacco industry, all gene editing materials are taken as objects, the materials are screened through chemical component analysis, and varieties with strong industrial availability are determined through compatibility test of tobacco leaves and verification of cigarette formulas, so that the tobacco variety is the key technical content which is urgently required in the tobacco industry.
The continuous flow analysis technology pumps standard working liquid, a sample and a reagent into a chemical analyzer in proportion, and simultaneously feeds gas at regular time to form a continuous fluid separation system in a pipeline, so that substances participating in reaction are fully mixed in the continuous flow process, then a series of reactions such as mixing, heating, filtering and the like are carried out, finally a corresponding peak value electric signal is obtained through detection of a detector, and then a corresponding concentration is obtained through automatic calculation of a standard curve. The continuous flow analysis technology has the advantages of high detection speed, small using amount of samples and reagents, capability of simultaneously detecting a plurality of components by one-time sample introduction and the like, and is widely applied to the tobacco industry at home and abroad.
① the traditional sample weighing process has the defects of heavy workload, complexity and time consumption, and serious influence on the detection work progress, ② the step of transferring the extractant, if a pipette is used as a measuring instrument, the labor intensity is high, the time consumption is long, and the transfer volume of the extractant is easy to be inconsistent due to unskilled operation personnel, so that the detection result is influenced, if a quantitative device is additionally arranged on a reagent bottle, air bubbles are easy to generate when the liquid is taken by a hand pressing method, so that the transfer volume of the extractant is inaccurate, and the detection result is also influenced, in ③ the pretreatment, the sample needs to be weighed into a ground triangular bottle, the extractant is added for oscillating extraction, the filtrate is filtered by qualitative filter paper, the filtrate is transferred into a sample inlet pipe of a continuous flow analyzer, and then the subsequent measurement can be carried out, in the filtering process, the mixture of solid sample residues and the extracting solution in the extracting bottle needs to be poured into a funnel with a qualitative folding bottle mouth, the filtered extracting solution is collected in a collecting bottle, the sample needs to be transferred for several times in the sample pretreatment process, the detection efficiency is not simple, and the detection efficiency is influenced, and the detection reliability of the extracting solution and impurities possibly caused by carelessness in the filtering process.
At least tens of thousands of edited materials can be generated in gene editing and industrial breeding, the daily sample processing amount of conventional chemical component analysis is only about 60 at present, a long period is needed for completing the conventional chemical component analysis of the tens of thousands of materials, and the lag of an analysis result can influence the progress of the whole industrial breeding, so that the requirement on an accurate and reliable high-throughput rapid analysis method in the gene editing and breeding process is very urgent.
The utility model discloses urgent demand to the quick analysis of conventional chemical composition of gene editing material, the quick analysis method of the high flux of the conventional chemical composition analysis of gene editing material has carried out the research, under the accurate prerequisite of assurance analysis results, through sample pretreatment process's improvement and automation, makes the daily sample analysis volume of conventional chemical composition improve more than 400, has improved work efficiency greatly, provides powerful guarantee for the chemical screening of gene editing batch production breeding material.
SUMMERY OF THE UTILITY MODEL
The utility model aims to provide a be used for a plurality of sample pretreatment devices of continuous flow analysis and application method thereof. The specific content is realized as follows:
the utility model discloses a processing apparatus before a plurality of samples for continuous flow analysis is disclosed in first aspect, including following part:
a support 2, substantially L-shaped, having a bottom wall 21 and a side wall 22;
the inside and the outside of the bottom wall of the extraction tube a are flat bottoms, and a plurality of sealing rings a1 are arranged at the tube openings;
the extraction pipe frame 3 is roughly cuboid, is placed on the bottom wall 21 of the bracket 2, and is provided with three parallel transverse plates, wherein a plurality of holes are uniformly distributed on the upper transverse plate 31 and the middle transverse plate 32 and used for placing the extraction pipes a, and the bottom outer walls of the extraction pipes a abut against the upper wall surface of the bottom transverse plate 33 of the extraction pipe frame 3;
the outer diameter of the filtering pipe b is matched with the inner diameter of the extracting pipe a, the upper end face of the filtering pipe b is a plane and protrudes in the radial direction, and the lower end face of the filtering pipe b is a filtering end;
the filter pipe frame 4 is approximately cuboid and is fixed on the side wall 22 of the bracket 2, and a plurality of holes are uniformly distributed on the filter pipe frame for placing the filter pipes b; the filter pipe frame 4 is arranged at the upper part of the extraction pipe frame 3, and the filter pipes b correspond to the extraction pipes a one by one;
and the lifting handle 5 is arranged on the side wall 22 of the bracket 2 and is used for controlling the lifting of the filter pipe frame 4.
Preferably, the following components are also included:
the multi-channel automatic liquid adding device 1 is matched with the plurality of extraction tubes a and corresponds to the extraction tubes a one by one;
the reagent storage bottle 7 is communicated and connected with the multi-channel automatic liquid adding device 1;
and a sample tray 6 having a substantially rectangular parallelepiped shape on which a plurality of sample tubes are placed.
Preferably, the following components are also included:
the liquid guide pipe d is positioned in the filter pipe b, the lower end of the liquid guide pipe d is provided with an expansion port which is fixedly connected with the inner wall of the lower end of the filter pipe b in a sealing way, and the upper end of the liquid guide pipe d extends out of the upper end surface of the filter pipe b;
a filter pipe cover plate e arranged on the upper part of the filter pipe frame 4 and capable of lifting along with the filter pipe frame 4; the filter tube cover plate e is provided with a hole so that the upper end of the liquid guide tube d extends out;
and the sieve plates c are positioned in the lower end openings of the filter pipes b and are one or more.
Preferably, the multi-channel automatic liquid filler 1 can realize the setting of the liquid adding volume, the automatic liquid adding and the accurate positioning of the liquid adding guide pipe.
Preferably, the sample tray 6 is plural.
Preferably, the pore size of the sieve plate c is 0.45 μm.
Preferably, the number of the plurality of extraction tubes a is 60.
The utility model discloses in a second aspect a method for using a processing apparatus before a plurality of samples of continuous flow analysis, including following step:
respectively placing a plurality of extraction tubes a into 3 holes of an extraction tube rack, respectively weighing samples and placing the samples into the extraction tubes a, setting the volume of a reagent to be added into each extraction tube a, respectively adding the reagent in a reagent storage bottle 13 into the extraction tubes a by using a multi-channel automatic liquid adding device 1, and sleeving a sealing ring a 1;
placing the extraction pipe frame 3 on an oscillator, performing oscillation extraction according to oscillation extraction conditions selected by a standard method, and then placing the extraction pipe frame 3 on the bottom wall 21 of the bracket 2;
sequentially placing a plurality of filter tubes b on a filter tube frame 4, inserting the upper ends of liquid guide tubes d into the sample tubes in the sample tray 6 in a one-to-one correspondence manner, covering a filter tube cover plate e, and rotating a lifting handle 5 to enable the filter tube frame 4 to gradually descend until the filter tubes b are inserted into the mouths of extraction tubes a in a one-to-one correspondence manner;
the lifting handle 5 is continuously rotated to press the filter tubes b downwards, so that the sample solution extracted from the plurality of extraction tubes a is filtered through the sieve plates c in the filter tubes b and enters the liquid guide tube d, and flows out of the sample tubes in the sample tray 6 through the liquid guide tube d;
the sample plate 6 is placed in a continuous flow analyzer and assayed according to conditions selected by standard methods. The general standard is a tobacco industry standard or an enterprise standard, such as YC/T159- Paper hexavalent chromium determination continuous flow method), and the like.
The beneficial effects of the utility model
1. The utility model discloses a processing apparatus reasonable in design before being used for a plurality of samples of continuous flow analysis, whole operation flow need not shift the sample, and sample pretreatment process has obtained simplifying greatly, shifts the reduction of step moreover and can effectively prevent the mistake in the sample pretreatment operation process, greatly reduced human error's introduction.
2. The utility model discloses a simultaneous processing that is used for a plurality of sample pretreatment device of continuous flow analysis can realize a plurality of samples. In order to reduce and shift, the utility model designs an oscillator with the extraction pipe support matching, directly place the oscillator on with the extraction pipe that extraction pipe support and reagent are equipped with on the frame and carry out the oscillation extraction, the extraction finishes the back and filters through the sieve on the filter tube, then will obtain the whole sample dish of filtrate and put on the continuous flow analysis appearance and carry out the analysis. The whole operation flow does not need to transfer the sample, the sample pretreatment process is greatly simplified, errors in the sample pretreatment operation process can be effectively prevented due to the reduction of the transfer steps, and the introduction of human errors is greatly reduced.
3. The utility model discloses a filter when being used for a plurality of sample pretreatment device of continuous flow analysis can realize a plurality of samples. Filter in order to realize a plurality of samples, the utility model discloses a steerable filter tube support and the ascending lifting handle who descends of filter tube apron, through the rising of filter tube support and filter tube apron and can realize the filtration of a plurality of (60) sample extraction liquid simultaneously with descending, saved sample pretreatment time and manual operation intensity greatly, show improvement work efficiency, sample analysis efficiency compares with present tobacco industry standard method and can improve several times.
4. The utility model discloses a be used for a plurality of samples of continuous flow analysis pretreatment device adopts the automatic liquid feeder of multichannel, and the liquid feeding degree of accuracy is high, and good repeatability only needs to press a button, and reagent can add as required, and is low to the operation technical requirement, and no artificial operation error, no reagent is extravagant, and labour saving and time saving practices thrift the personnel selection cost greatly.
5. The utility model discloses a be used for a plurality of samples of continuous flow analysis processing apparatus design benefit reasonable, whole operation flow need not shift the sample, and sample pretreatment process has obtained simplifying greatly, has shortened the time greatly, is showing and is improving work efficiency. More than two sample disks are used alternately, uninterrupted analysis can be carried out, 8-10 disks of samples can be processed by 2 people every day, more than 400 samples can be analyzed every day according to the conventional sample introduction speed of 60 samples/h, and compared with the current standard method in the tobacco industry, the sample analysis efficiency can be improved by multiple times. The utility model provides a high-flux rapid analysis method for screening massive editing materials generated by gene industrial breeding, and greatly accelerates the analysis process of conventional chemical components in the screening process of the gene editing materials.
Drawings
Fig. 1 is a schematic view of the extraction tube and the filtration tube of the present invention.
Fig. 2 is a schematic view of the device for processing a plurality of samples in a continuous flow analysis according to the present invention.
Fig. 3 is a schematic view of the multi-channel automatic liquid adding device and the reagent storage bottle of the present invention.
Reference numerals: 1. a multi-channel automatic liquid feeder; 2. a support; 21. a bottom wall; 22. a side wall; 3. an extraction pipe frame; 31. an upper transverse plate; 32. a middle horizontal plate; 33. a bottom transverse plate; 4. a filter pipe frame; 5. a lifting handle; 6. A sample tray; 7. a reagent storage bottle;
a. an extraction tube; a1, sealing ring; b. a filter tube; c. a sieve plate; d. a catheter; e. and a filter tube cover plate.
Detailed Description
The present invention is further described by the following specific examples, but the present invention is not limited thereto.
The utility model discloses a be used for a plurality of sample preprocessing devices of continuous flow analysis as shown in fig. 1-3, including following part:
a support 2, substantially L-shaped, having a bottom wall 21 and a side wall 22; the inside and the outside of the bottom wall of the extraction tube a are flat bottoms, and 60 sealing rings a1 are arranged at the tube openings; the extraction pipe frame 3 is roughly cuboid, is placed on the bottom wall 21 of the bracket 2, and is provided with three parallel transverse plates, wherein a plurality of holes are uniformly distributed on the upper transverse plate 31 and the middle transverse plate 32 and used for placing the extraction pipes a, and the bottom outer walls of the extraction pipes a abut against the upper wall surface of the bottom transverse plate 33 of the extraction pipe frame 3; the outer diameter of the filter tubes b is matched with the inner diameter of the extraction tube a, the number of the filter tubes b is also 60, the upper end surfaces of the filter tubes b are planes and protrude radially, and the lower end surfaces of the filter tubes b are filter ends; the filter pipe frame 4 is approximately cuboid and is fixed on the side wall 22 of the bracket 2, and a plurality of holes are uniformly distributed on the filter pipe frame for placing the filter pipes b; the filter pipe frame 4 is arranged at the upper part of the extraction pipe frame 3, and the filter pipes b correspond to the extraction pipes a one by one; and the lifting handle 5 is arranged on the side wall 22 of the bracket 2 and is used for controlling the lifting of the filter pipe frame 4.
The multi-channel automatic liquid adding device 1 is matched with the plurality of extraction tubes a and corresponds to the extraction tubes a one by one; the reagent storage bottle 7 is communicated and connected with the multi-channel automatic liquid adding device 1; and a plurality of sample trays 6 having a substantially rectangular parallelepiped shape on which 60 sample tubes are placed. The multi-channel automatic liquid feeder 1 can realize the setting of the liquid feeding volume, the automatic liquid feeding and the accurate positioning of the liquid feeding guide pipe.
The liquid guide pipe d is positioned in the filter pipe b, the lower end of the liquid guide pipe d is provided with an expansion port which is fixedly connected with the inner wall of the lower end of the filter pipe b in a sealing way, and the upper end of the liquid guide pipe d extends out of the upper end surface of the filter pipe b;
a filter pipe cover plate e arranged on the upper part of the filter pipe frame 4 and capable of lifting along with the filter pipe frame 4; the filter tube cover plate e is provided with a hole so that the upper end of the liquid guide tube d extends out; and one or more sieve plates c are positioned in the lower end opening of the filter pipe b, and the pore diameter of the sieve plates c is 0.45 mu m.
Example (b): determination of total sugar in flue-cured tobacco sample
Sample preparation: firstly, the tobacco leaves (or tobacco shreds) are dried, the high-speed cyclone mill is used for breaking the cell wall of the tobacco and tobacco products, then the tobacco and tobacco products are screened by a 60-mesh screen, and the tobacco and tobacco products are sealed and stored for preparing samples. The water content of the samples was measured according to YC/T31-1996 standard. The following treatments were carried out:
A. respectively placing 60 extraction tubes into extraction tube rack holes, respectively weighing 0.25g of tobacco leaf (or tobacco shred) samples, placing the tobacco leaf (or tobacco shred) samples into the extraction tubes, setting the volume of each extraction tube to be added with a reagent, respectively adding the reagents in a reagent storage bottle into the extraction tubes by using a multi-channel automatic liquid adding device, and sleeving sealing rings on the extraction tubes;
B. placing the extraction pipe frame on an oscillator, performing oscillation extraction according to oscillation extraction conditions selected by a standard method, and then placing the extraction pipe frame on the bottom wall of the bracket;
C. sequentially placing 60 filter tubes on a filter tube frame, inserting the upper ends of liquid guide tubes into the sample tubes in the sample tray in a one-to-one correspondence manner, covering a filter tube cover plate, and rotating a lifting handle to enable the filter tube frame to gradually descend until the filter tubes are inserted into the openings of the extraction tubes in a one-to-one correspondence manner;
D. the lifting handle is continuously rotated to press the filter pipes downwards, so that the sample solution extracted from the 60 extraction pipes simultaneously enters the liquid guide pipe through the sieve plates in the filter pipes and flows out of the 60 sample pipes in the sample tray through the liquid guide pipe;
E. the sample plate is placed in a continuous flow analyzer and the assay is performed under conditions selected by standard methods.
The above process takes about 2 hours to complete, whereas the prior art test method takes about 1 day and the efficiency is improved by a factor of 4-6.
Claims (7)
1. A pretreatment apparatus for continuous flow analysis of a plurality of samples, comprising:
a bracket (2) which is approximately L-shaped and is provided with a bottom wall (21) and a side wall (22);
the inner and outer bottom walls of the extraction tube (a) are flat, and a plurality of sealing rings (a1) are arranged at the tube openings;
the extraction pipe frame (3) is approximately cuboid, is placed on the bottom wall (21) of the bracket (2) and is provided with three parallel transverse plates, wherein a plurality of holes are uniformly distributed on the upper transverse plate (31) and the middle transverse plate (32) for placing the extraction pipes (a), and the bottom outer walls of the extraction pipes (a) are abutted against the upper wall surface of the bottom transverse plate (33) of the extraction pipe frame (3);
the outer diameter of the filtering pipe (b) is matched with the inner diameter of the extracting pipe (a), the upper end surface of the filtering pipe (b) is a plane and protrudes radially, and the lower end surface of the filtering pipe (b) is a filtering end;
the filter pipe frame (4) is approximately cuboid and is fixed on the side wall (22) of the bracket (2), and a plurality of holes are uniformly distributed on the filter pipe frame for placing the filter pipes (b); the filter pipe frame (4) is arranged at the upper part of the extraction pipe frame (3) and enables the filter pipes (b) and the extraction pipes (a) to correspond one to one;
and the lifting handle (5) is arranged on the side wall (22) of the support (2) and used for controlling the lifting of the filter pipe frame (4).
2. The apparatus of claim 1, further comprising the following components:
the multi-channel automatic liquid adding device (1) is matched with the plurality of extraction tubes (a) and corresponds to the extraction tubes one by one;
the reagent storage bottle (7) is communicated and connected with the multi-channel automatic liquid adding device (1);
a sample tray (6) having a substantially rectangular parallelepiped shape on which a plurality of sample tubes are placed.
3. The apparatus of claim 1, further comprising the following components:
the liquid guide pipe (d) is positioned in the filter pipe (b), the lower end of the liquid guide pipe is provided with an expansion port which is fixedly connected with the inner wall of the lower end of the filter pipe (b) in a sealing way, and the upper end of the liquid guide pipe extends out of the outer part of the upper end surface of the filter pipe (b);
the filter pipe cover plate (e) is arranged at the upper part of the filter pipe frame (4) and can lift along with the filter pipe frame (4); the filter tube cover plate (e) is provided with a hole so that the upper end of the liquid guide tube (d) extends out;
and the sieve plates (c) are positioned in the lower end openings of the filter pipes (b) and are one or more.
4. The device for the pre-treatment of multiple samples for continuous flow analysis according to claim 2, wherein the multi-channel automatic liquid charger (1) can realize the setting of liquid charging volume, automatic liquid charging and the accurate positioning of a liquid charging conduit.
5. The multiple sample preparation device for continuous flow analysis according to claim 2, wherein the sample tray (6) is provided in plurality.
6. The multiple sample pretreatment device for continuous flow analysis according to claim 3, wherein the sieve plate (c) has a pore size of 0.45 μm.
7. The multiple sample pretreatment device for continuous flow analysis according to claim 1, wherein the number of said plurality of extraction tubes (a) is 60.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110398606A (en) * | 2019-07-24 | 2019-11-01 | 云南中烟工业有限责任公司 | One kind being used for the multiple sample pretreatment devices of Continuous Flow Analysis and its application method |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110398606A (en) * | 2019-07-24 | 2019-11-01 | 云南中烟工业有限责任公司 | One kind being used for the multiple sample pretreatment devices of Continuous Flow Analysis and its application method |
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