CN109042765A - A kind of pharmaceutical composition and preparation method thereof of environmentally protective environment disinfectant - Google Patents
A kind of pharmaceutical composition and preparation method thereof of environmentally protective environment disinfectant Download PDFInfo
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- CN109042765A CN109042765A CN201811147632.5A CN201811147632A CN109042765A CN 109042765 A CN109042765 A CN 109042765A CN 201811147632 A CN201811147632 A CN 201811147632A CN 109042765 A CN109042765 A CN 109042765A
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- edta
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- pharmaceutical composition
- protective environment
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/44—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a nitrogen atom attached to the same carbon skeleton by a single or double bond, this nitrogen atom not being a member of a derivative or of a thio analogue of a carboxylic group, e.g. amino-carboxylic acids
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/34—Rosaceae [Rose family], e.g. strawberry, hawthorn, plum, cherry, peach, apricot or almond
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Dentistry (AREA)
- Agronomy & Crop Science (AREA)
- Environmental Sciences (AREA)
- Wood Science & Technology (AREA)
- Plant Pathology (AREA)
- Mycology (AREA)
- Biotechnology (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Microbiology (AREA)
- Pest Control & Pesticides (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention discloses a kind of pharmaceutical compositions of environmentally protective environment disinfectant of environment disinfected technical field, the drug is by with antibacterial and elimination R-plasmid hawthorn, bacterial cell membrane permeability promotor ethylenediamine tetra-acetic acid (EDTA), the two combines by a certain percentage, EDTA is diluted by a., plus medical fluid b., c. it saves, d. Bacteria Culture, plus bacterium solution e., f. culture and g. observation result, it filters out according to Chinese veterinarian's theory to the apparent water extract of TCM of environment disinfected effect, according to the characteristics of the antibacterial and reversing drug resistance of hawthorn and principle, with with certain density EDTA, improve the Disinfection Effect of hawthorn, reach antibacterial and reversing drug resistance double effects.
Description
Technical field
The present invention relates to environment disinfected technical field, the pharmaceutical composition of specially a kind of environmentally protective environment disinfectant and its
Preparation method.
Background technique
Most of microorganism in air is since nutritional deficiency and solar irradiation within a short period of time can be dead, but once
Biomembrane is formed in conjunction with the carriers such as steam, dust, bacterium resists the influence of extraneous poor environment by forming biomembrane, makes
Bacterium can preferably survive.Bacterium can be improved 10-1000 times to the tolerance of antibacterials after forming biomembrane, serious shadow
Ring the therapeutic effect of antibiotic.Research has shown that biomembrane is one of the killer for threatening human health.It is counted according to CDC, Ren Leiyue
65% bacterial infection disease is related with biofilm formation.The eruption and prevalence of bacteriosis is mutually propagated with gas source to be had
It closes, so breeding environment will be sterilized periodically.
The long-time service of Environmental Chemistry disinfectant not only causes the generation of bacterial drug resistance, but also can cause dirt to environment
Dye.We have discovered that environment Escherichia coli are different to povidone iodine, glutaraldehyde, glutaraldehyde Deciquam, bromogeramine, dichloro
There are multidrug resistant phenomenons for the common chemical disinfection medicine such as NaDCC.Moreover, there is corrosion or thorns for these chemical disinfection medicines
There are strict requirements when the disadvantages of swashing property, especially Continuous disinfection.In view of the seriousness and status of problem, finds and research and development more have
Effect and nontoxic new disinfectant become inevitable, for this purpose, it is proposed that a kind of pharmaceutical composition of environmentally protective environment disinfectant and
Preparation method.
Summary of the invention
The purpose of the present invention is to provide a kind of pharmaceutical compositions and preparation method thereof of environmentally protective environment disinfectant, with solution
Certainly the problems mentioned above in the background art.
To achieve the above object, the invention provides the following technical scheme: a kind of medicine group of environmentally protective environment disinfectant
It closes, which is by with antibacterial and elimination R-plasmid hawthorn, bacterial cell membrane permeability promotor ethylenediamine tetra-acetic acid
(EDTA), the two combines by a certain percentage.
Preferably, a kind of preparation method of environmentally protective environment disinfectant, this method comprises the following steps:
A. it dilutes EDTA: selected EDTA is diluted according to multiple proportions；
B. plus medical fluid: the EDTA solution of various concentration after doubling dilution is added separately to 96 hole polystyrene plates of sterilizing
In, the 1st to the 10th hole adds medical fluid, every 10 μ l of hole, and the 11st hole is culture medium, and the 12nd hole not dosing is used as growth control；
C. it saves: after step b, 96 hole polystyrene plates being carried out freezing dried sealing, are saved backup；
D. Bacteria Culture: it will be diluted to certain density Escherichia coli respectively, salmonella type strain is added to 5ml nutrition
In meat soup, constant-temperature table culture then is carried out to it；
Plus bacterium solution e.: adjustment bacterial concentration is 1 × 108, bacteria suspension is added in the MIC plate prepared, every hole is added
100ml；
F. cultivate: after liquid feeding, concussion is mixed, and is placed and is cultivated in incubator；
G. observe result: after step f, check and acceptance result, with the minimum dilution of asepsis growth i.e. be set to MIC value or
MBC value.
Preferably, in step e, in EDTA 1 hole of density control to the 12nd hole be respectively 2048,1024,512,256,128,64,
32、16、8、4、2、1ug/mL。
Preferably, positive control and blank control, and every group of three repetitions are equipped in step e.
Preferably, the temperature of incubator is 37 DEG C in step f, and incubation time is for 24 hours.
Preferably, the temperature of constant-temperature table culture is 37 DEG C in step d, incubation time 4.5h.
Compared with prior art, the beneficial effects of the present invention are: the present invention provides a kind of pharmaceutical composition of environment disinfectant
Object is filtered out according to Chinese veterinarian's theory with antibacterial and elimination R-plasmid hawthorn, bacterial cell membrane permeability promotor
EDTA, the two combine by a certain percentage, filter out to the apparent water extract of TCM of environment disinfected effect, according to the antibacterial of hawthorn and
The characteristics of reversing drug resistance and principle, with and certain density EDTA, improve the Disinfection Effect of hawthorn, reach antibacterial and reversing drug resistance
Double effects.
Specific embodiment
Below in conjunction with the embodiment of the present invention, technical scheme in the embodiment of the invention is clearly and completely described,
Obviously, described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.Based in the present invention
Embodiment, every other embodiment obtained by those of ordinary skill in the art without making creative efforts, all
Belong to the scope of protection of the invention.
Embodiment 1
The present invention provides a kind of technical solution: a kind of pharmaceutical composition of environmentally protective environment disinfectant, which is by having
Have antibacterial and eliminate the hawthorn of R-plasmid, bacterial cell membrane permeability promotor ethylenediamine tetra-acetic acid (EDTA), the two presses one
Certainty ratio combination；
A kind of preparation method of environmentally protective environment disinfectant, this method comprises the following steps:
A. it dilutes EDTA: selected EDTA is diluted according to multiple proportions；
B. plus medical fluid: the EDTA solution of various concentration after doubling dilution is added separately to 96 hole polystyrene plates of sterilizing
In, the 1st to the 10th hole adds medical fluid, every 10 μ l of hole, and the 11st hole is culture medium, and the 12nd hole not dosing is used as growth control；
C. it saves: after step b, 96 hole polystyrene plates being carried out freezing dried sealing, are saved backup；
D. Bacteria Culture: it will be diluted to certain density Escherichia coli respectively, salmonella type strain is added to 5ml nutrition
In meat soup, constant-temperature table culture then is carried out to it；
Plus bacterium solution e.: adjustment bacterial concentration is 1 × 108, bacteria suspension is added in the MIC plate prepared, every hole is added
100ml；
F. cultivate: after liquid feeding, concussion is mixed, and is placed and is cultivated in incubator；
G. observe result: after step f, check and acceptance result, with the minimum dilution of asepsis growth i.e. be set to MIC value or
MBC value；
In step e, in EDTA 1 hole of density control to the 12nd hole be respectively 2048,1024,512,256,128,64,32,16,
8,4,2,1ug/mL, convenient for obtaining minimal inhibitory concentration, EDTA is to Escherichia coli type strain ATCC25922, Salmonella after measured
The minimal inhibitory concentration of bacterium type strain CMCC (B) 50071 is 512ug/ml；
It is equipped with positive control and blank control, and every group of three repetitions in step e, prepares the accurate of medical fluid convenient for raising
Property；
The temperature of incubator is 37 DEG C in step f, and incubation time is for 24 hours；
The temperature of constant-temperature table culture is 37 DEG C, incubation time 4.5h in step d, convenient for being incubated for bacterium.
Natural sedimentation: in same chicken farm, 4 henhouses are chosen, every henhouse chooses 3 sampled points, from the ground height
It about 1.5 meters, is evenly distributed, measures clump count according to natural sedimentation operating method.4 groups of progress of test point, I group is glutaraldehyde
Deciquam disinfection, II group is sterilized using povidone iodine, and III group is disappeared using EDTA 512ug/ml, hawthorn water extract 0.04g/ml
Poison, IV group is sterilized using EDTA 512ug/ml, hawthorn water extract 0.08g/ml, and V group is EDTA 512ug/ml, hawthorn water mentions
Object 0.16g/ml disinfection, 3 repetitions of each sampled point.Commercially available disinfectant, which uses, recommends dosage, and hawthorn water extract disinfection concentration is
2MIC compares the clump count of disinfection front and back with same method statistic clump count to henhouse spraying disinfection.
Hawthorn water extract is to environment of chicken house Disinfection Effect
Test result explanation: pharmaceutical composition EDTA 512ug/ml, hawthorn water extract 0.08g/ml and pharmaceutical composition EDTA
512ug/ml, hawthorn water extract 0.16g/ml Disinfection Effect be better than povidone iodine and glutaraldehyde Deciquam.
It although an embodiment of the present invention has been shown and described, for the ordinary skill in the art, can be with
A variety of variations, modification, replacement can be carried out to these embodiments without departing from the principles and spirit of the present invention by understanding
And modification, the scope of the present invention is defined by the appended.
Claims (6)
1. a kind of pharmaceutical composition of environmentally protective environment disinfectant, it is characterised in that: the drug is by resistance to antibacterial and elimination
The hawthorn of medicine plasmid, bacterial cell membrane permeability promotor ethylenediamine tetra-acetic acid (EDTA), the two combines by a certain percentage.
2. a kind of preparation method of environmentally protective environment disinfectant, it is characterised in that: this method comprises the following steps:
A. it dilutes EDTA: selected EDTA is diluted according to multiple proportions；
Plus medical fluid b.: the EDTA solution of various concentration after doubling dilution is added separately in 96 hole polystyrene plates of sterilizing,
1st to the 10th hole adds medical fluid, every 10 μ l of hole, and the 11st hole is culture medium, and the 12nd hole not dosing is used as growth control；
C. it saves: after step b, 96 hole polystyrene plates being carried out freezing dried sealing, are saved backup；
D. Bacteria Culture: it will be diluted to certain density Escherichia coli respectively, salmonella type strain is added to 5ml nutrient broth
In, constant-temperature table culture then is carried out to it；
Plus bacterium solution e.: adjustment bacterial concentration is 1 × 108, bacteria suspension is added in the MIC plate prepared, 100ml is added in every hole；
F. cultivate: after liquid feeding, concussion is mixed, and is placed and is cultivated in incubator；
G. observe result: after step f, check and acceptance result is set to MIC value or MBC value with the minimum dilution of asepsis growth.
3. a kind of pharmaceutical composition of environmentally protective environment disinfectant according to claim 2, it is characterised in that: in step e,
1 hole of density control to the 12nd hole is respectively 2048,1024,512,256,128,64,32,16,8,4,2,1ug/mL in EDTA.
4. a kind of pharmaceutical composition of environmentally protective environment disinfectant according to claim 2, it is characterised in that: in step e
Equipped with positive control and blank control, and every group of three repetitions.
5. a kind of pharmaceutical composition of environmentally protective environment disinfectant according to claim 2, it is characterised in that: in step f
The temperature of incubator is 37 DEG C, and incubation time is for 24 hours.
6. a kind of pharmaceutical composition of environmentally protective environment disinfectant according to claim 2, it is characterised in that: in step d
The temperature of constant-temperature table culture is 37 DEG C, incubation time 4.5h.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811147632.5A CN109042765A (en) | 2018-09-29 | 2018-09-29 | A kind of pharmaceutical composition and preparation method thereof of environmentally protective environment disinfectant |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811147632.5A CN109042765A (en) | 2018-09-29 | 2018-09-29 | A kind of pharmaceutical composition and preparation method thereof of environmentally protective environment disinfectant |
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| Publication Number | Publication Date |
|---|---|
| CN109042765A true CN109042765A (en) | 2018-12-21 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
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| CN201811147632.5A Pending CN109042765A (en) | 2018-09-29 | 2018-09-29 | A kind of pharmaceutical composition and preparation method thereof of environmentally protective environment disinfectant |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112616836A (en) * | 2020-12-18 | 2021-04-09 | 湖南幻影三陆零科技有限公司 | Compound disinfectant, disinfection composition and application thereof |
| CN113604535A (en) * | 2021-08-03 | 2021-11-05 | 河北工程大学 | Traditional Chinese medicine formula for eliminating drug resistance of escherichia coli and screening method and application thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100998886A (en) * | 2007-01-08 | 2007-07-18 | 广东省微生物研究所 | Indoor air disinfectant |
| CN103242279A (en) * | 2013-05-16 | 2013-08-14 | 陕西科技大学 | Method for extracting and purifying flavonoid substances in hawthorn |
| CN107969424A (en) * | 2017-11-23 | 2018-05-01 | 赤峰中农大生化科技有限责任公司 | A kind of insecticide containing Radix Stemonae extract |
-
2018
- 2018-09-29 CN CN201811147632.5A patent/CN109042765A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100998886A (en) * | 2007-01-08 | 2007-07-18 | 广东省微生物研究所 | Indoor air disinfectant |
| CN103242279A (en) * | 2013-05-16 | 2013-08-14 | 陕西科技大学 | Method for extracting and purifying flavonoid substances in hawthorn |
| CN107969424A (en) * | 2017-11-23 | 2018-05-01 | 赤峰中农大生化科技有限责任公司 | A kind of insecticide containing Radix Stemonae extract |
Non-Patent Citations (4)
| Title |
|---|
| 张永英 等: "EDTA对气载大肠杆菌消毒剂抗性的影响", 《中国家禽》 * |
| 肖莉春 等: "中药提取物对猪源耐药大肠杆菌分离株的抑制作用", 《江西农业大学学报》 * |
| 鞠洪涛 等: "中草药消除大肠埃希氏菌耐药性及耐药质粒的研究", 《中国兽医科技》 * |
| 高愿军 等: "抗氧化剂对控制山楂加工中Vc损失的效应", 《食品工业科技》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112616836A (en) * | 2020-12-18 | 2021-04-09 | 湖南幻影三陆零科技有限公司 | Compound disinfectant, disinfection composition and application thereof |
| CN113604535A (en) * | 2021-08-03 | 2021-11-05 | 河北工程大学 | Traditional Chinese medicine formula for eliminating drug resistance of escherichia coli and screening method and application thereof |
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Application publication date: 20181221 |