CN109031637A - A kind of light-source system suitable for high-velocity scanning - Google Patents
A kind of light-source system suitable for high-velocity scanning Download PDFInfo
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- CN109031637A CN109031637A CN201810896970.2A CN201810896970A CN109031637A CN 109031637 A CN109031637 A CN 109031637A CN 201810896970 A CN201810896970 A CN 201810896970A CN 109031637 A CN109031637 A CN 109031637A
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- 238000003384 imaging method Methods 0.000 claims abstract description 72
- 238000005286 illumination Methods 0.000 claims abstract description 63
- 239000005338 frosted glass Substances 0.000 claims abstract description 40
- 125000006850 spacer group Chemical group 0.000 claims description 12
- 239000005337 ground glass Substances 0.000 claims description 6
- 210000001747 pupil Anatomy 0.000 claims description 5
- 230000015572 biosynthetic process Effects 0.000 claims 2
- 230000003760 hair shine Effects 0.000 claims 1
- 238000000034 method Methods 0.000 abstract description 13
- 230000008569 process Effects 0.000 abstract description 10
- 230000000875 corresponding effect Effects 0.000 description 21
- 230000006872 improvement Effects 0.000 description 12
- 230000003287 optical effect Effects 0.000 description 6
- 239000011521 glass Substances 0.000 description 5
- 230000004075 alteration Effects 0.000 description 3
- 238000010586 diagram Methods 0.000 description 3
- 230000004313 glare Effects 0.000 description 3
- 239000003086 colorant Substances 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000002093 peripheral effect Effects 0.000 description 1
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- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/06—Means for illuminating specimens
- G02B21/08—Condensers
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- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/0004—Microscopes specially adapted for specific applications
- G02B21/002—Scanning microscopes
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- Microscoopes, Condenser (AREA)
Abstract
The invention discloses a kind of light-source systems suitable for high-velocity scanning, belong to medical instruments field, collecting lens, two-sided frosted glass, illumination field lens and condenser are coaxially disposed by corresponding light source, form " double light path " light-source system, it can effectively realize the accurate illumination in sample high-velocity scanning imaging process to sample, and passing through preferred light source is tetra- color LED light of RGBW, so that the achievable fast imaging of light-source system and high quality imaging.Light-source system suitable for high-velocity scanning of the invention, its structure is simple, control is easy, can effectively promote the illumination quality in scan sample imaging process, and then promote the quality of scan sample imaging, and the control form multiplicity of light-source system, it is not only able to achieve fast imaging, high quality imaging is also able to achieve, effectively improves the functionality and application range of light-source system, the cost of scanning imagery is reduced, there is highly important application value.
Description
Technical Field
The invention belongs to the field of medical instruments, and particularly relates to a light source system suitable for high-speed scanning.
Background
In the field of biological medicine, the application of a microscopic scanning imaging device is very wide, in the application process, a sample to be microscopically scanned is often required to be placed on an objective table, the objective table drives the sample to move along an x axis and/or a y axis, so that each part of the sample can sequentially pass through a focusing plane of an objective lens, then each point on the sample is respectively imaged through the objective lens, and finally the imaging of each point is spliced to obtain the image of the whole sample, thereby completing the microscopic scanning of the sample.
At present, the microscopic scanning of a sample is often completed through the cooperation of a microscopic imaging device and a light source system, namely, before scanning, the sample is fixedly placed on an objective table, the positioning and focusing of the sample are realized through the movement of the objective table along an x axis and/or a y axis and the continuous focusing of a camera, and the sample is correspondingly irradiated on the sample through the light source system, so that the clear display of a sample image is correspondingly completed.
In the microscopic scanning imaging process of a sample, the requirement on a light source system is high, and the imaging quality of the sample is directly influenced by the illumination quality of the sample; however, in the prior art, a light source in a micro-scanning imaging system is easily directly imaged on a sample, so that the sample is irradiated unevenly and insufficiently, and glare is easily generated, so that the imaging efficiency and quality of the sample are affected; in addition, most of the existing sample imaging forms are spatial color mixing methods, which can achieve microscopic imaging of a sample to a certain extent, but the scanning imaging of the sample by adopting the spatial color mixing method is easy to reduce the imaging quality of the sample due to the color difference of the objective lens, so that when the resolution requirement of the imaging device is high, the actual requirement is hardly met, high-quality and high-speed scanning of the sample cannot be effectively achieved, and certain limitations are provided.
Disclosure of Invention
Aiming at the defects or improvement requirements of the prior art, the invention provides a light source system suitable for high-speed scanning, which is characterized in that a light collecting lens, double-sided ground glass, a lighting field lens and a light collecting lens are coaxially arranged corresponding to a light source to form a double-light-path light source system, so that accurate illumination on a sample in the high-speed scanning imaging process of the sample can be effectively realized, the conditions of uneven light source illumination and insufficient sample illumination in the scanning imaging system are avoided, the generation of a glare phenomenon is reduced, the quality of sample illumination is improved, the scanning imaging quality of the sample is further improved, and the high-quality and high-speed scanning of the sample is realized.
In order to achieve the above object, the present invention provides 1. a light source system suitable for high-speed scanning, which is used for completing scanning imaging of a sample in cooperation with an imaging device, and is characterized in that the light source system comprises a light source, a light collecting lens, double-sided frosted glass, an illumination field lens and a light collecting lens which are coaxially arranged at intervals in sequence; wherein,
the light source is arranged away from the imaging device, arranged on one side of the light collecting mirror and used for generating light rays to irradiate on the light collecting mirror;
the light collecting lens is correspondingly arranged between the light source and the double-sided frosted glass and is used for collecting light rays generated by the light source and then transmitting the light rays to the double-sided frosted glass and the illumination field lens;
the double-sided frosted glass is arranged between the illumination field lens and the light collecting lens, light rays from the light collecting lens can correspondingly penetrate through the double-sided frosted glass and correspondingly irradiate on the illumination field lens to form a first light path, and the double-sided frosted glass can be illuminated and used as a secondary light source to emit light rays to the illumination field lens to form a second light path;
the illumination field lens is arranged corresponding to the collecting lens, so that the light rays of the first light path can be converged by the illumination field lens and then transmitted to the collecting lens, and the light rays of the second light path can be deflected by the illumination field lens and then correspondingly transmitted to the collecting lens;
the condenser lens is arranged corresponding to the imaging device and can be aligned with the sample, the light rays of the first light path are converted into parallel light to be irradiated on the sample, so that the light source can form images at the entrance pupil of the objective lens of the imaging device, and the light rays of the second light path can be converged at the sample after passing through the condenser lens, so that the images of the double-sided frosted glass at the sample are formed.
As a further improvement of the invention, a light source seat is coaxially arranged corresponding to the light source, and the light source can be correspondingly accommodated on the light source seat.
As a further improvement of the present invention, a light collecting lens barrel is provided corresponding to the light collecting lens, and the light collecting lens is coaxially fixed in the light collecting lens barrel.
As a further improvement of the present invention, the double-sided frosted glass is coaxially disposed in the light collecting lens barrel, and a first spacer is disposed between the double-sided frosted glass and the light collecting lens, and two ends of the first spacer are respectively abutted to the double-sided frosted glass and the light collecting lens.
As a further improvement of the invention, a field lens adjusting cylinder is arranged corresponding to the illumination field lens, and the illumination field lens is coaxially arranged at one end of the field lens adjusting cylinder, which is close to the condenser lens.
As a further improvement of the invention, a combined lens barrel with a hollow interior is arranged between the collecting lens and the illumination field lens, the collecting lens is correspondingly fixed at one end of the combined lens barrel, and the other end of the combined lens barrel is correspondingly connected with the end part of the field lens adjusting barrel.
As a further improvement of the present invention, one end of the light collecting lens barrel is provided with a first locking nut in a circular ring shape corresponding to the double-sided frosted glass, and the first locking nut can be connected with the end of the light collecting lens barrel in a threaded manner and abuts against the double-sided frosted glass with the end, so as to realize axial position limitation of the double-sided frosted glass.
As a further improvement of the present invention, a protrusion is circumferentially disposed on an inner circumferential wall of the field lens adjusting cylinder, one side of the field lens is correspondingly abutted against the protrusion, and a second spacer and a second lock nut are coaxially disposed on the other side of the field lens, the second lock nut can be correspondingly screwed to an end of the field lens adjusting cylinder, and two ends of the second spacer are respectively abutted against the second lock nut and the field lens.
As a further improvement of the invention, the light source is an RGBW four-color LED lamp.
As a further improvement of the present invention, the light collecting mirror is an aspheric light collecting lens.
As a further improvement of the invention, the condenser is an Abbe condenser.
Generally, compared with the prior art, the above technical solution conceived by the present invention has the following beneficial effects:
(1) according to the light source system suitable for high-speed scanning, the light collecting lens, the double-sided frosted glass, the illumination field lens and the light collecting lens are preferably and coaxially arranged corresponding to the light source to form the 'double-light-path' light source system, so that accurate illumination on a sample in the high-speed scanning imaging process of the sample is effectively realized, the conditions that the light source illumination is uneven and the sample illumination is insufficient in the scanning imaging system are avoided, the generation of a glare phenomenon is reduced, the illumination quality of the sample is improved, and the scanning imaging quality of the sample is further improved;
(2) according to the light source system suitable for high-speed scanning, the light source is provided with the RGBW four-color LED lamp, so that the light source has two illumination imaging modes, a color image can be formed through one-time imaging, the rapid imaging of a sample is effectively met, three monochromatic images can be imaged first, the three monochromatic images are synthesized into one color image, the self chromatic aberration of an objective lens is effectively eliminated, the high-quality imaging of the sample is met, the application range of the light source system is effectively expanded, and the efficiency and the quality of the high-speed scanning imaging of the sample are improved;
(3) according to the light source system suitable for high-speed scanning, the light collecting lens barrel is arranged corresponding to the light collecting lens and the double-sided frosted glass, the field lens adjusting barrel is arranged corresponding to the illumination field lens, and the combined lens barrel is arranged between the light collecting lens and the field lens adjusting barrel, so that stable arrangement of each component of the light source system is effectively realized, the position relation among all the components is ensured to meet the working requirement of the light source system all the time, the stability of the light source system is further improved, the illumination quality of a sample in the scanning imaging process is ensured, and the scanning quality and accuracy of the sample are further improved;
(4) the light source system suitable for high-speed scanning is simple in structure and convenient to control, can effectively improve the illumination quality in the sample scanning imaging process, further improves the quality of sample scanning imaging, is various in control form, can realize quick imaging and high-quality imaging, effectively improves the functionality and application range of the light source system, reduces the cost of scanning imaging, and has very important popularization and application values.
Drawings
FIG. 1 is a schematic diagram of an overall structure of a light source system suitable for high-speed scanning according to an embodiment of the present invention;
FIG. 2 is a schematic diagram of an imaging of an optical path A of a light source system in an embodiment of the present invention;
FIG. 3 is a schematic diagram of an imaging of the optical path B of the light source system in the embodiment of the present invention;
in all the figures, the same reference numerals denote the same features, in particular: 1. the light source, 2, the light collecting lens, 3, the double-sided frosted glass, 4, the illumination field lens, 5, the light collecting lens, 6, the combined lens barrel, 7, the field lens adjusting barrel, 8, the light collecting lens barrel, 9, the light source seat, 10, the glass slide, 11, the imaging device, 12, the first locking nut, 13, the second locking nut, 14, the first space ring and 15, the second space ring.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is described in further detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
In addition, the technical features involved in the embodiments of the present invention described below may be combined with each other as long as they do not conflict with each other.
A light source system suitable for high-speed scanning in the preferred embodiment of the invention is shown in FIGS. 1-3, wherein the light source system comprises a light source 1, a light collecting lens 2, double-sided frosted glass 3, an illumination field lens 4 and a light collecting lens 5 which are coaxially and sequentially arranged.
Specifically, the light source system in the preferred embodiment is preferably vertically arranged, as shown in fig. 1, the light source 1 is arranged at the lowermost end of the light source system, and the light source base 9 is arranged correspondingly thereto, that is, the light source 1 is correspondingly fixed on the light source base 9; further preferably, the light source 1 in the preferred embodiment is an RGBW four-color LED lamp; of course, the light source 1 may be other lamps, such as RGB three-color LED lamps, etc. according to actual needs.
Further, the light collecting mirror 2 in the preferred embodiment is disposed corresponding to the light source 1, is disposed coaxially with the light source 1, and is preferably disposed corresponding to the light collecting lens barrel 8, the light collecting lens barrel 8 in the preferred embodiment is cylindrical, and is hollow inside and has an annular boss corresponding to the light collecting mirror 2 on the inner peripheral wall, so that the light collecting mirror 2 can be correspondingly positioned on the annular boss by placing the edge thereof; further, one end of the light collecting lens barrel 8 is correspondingly matched and connected with the light source seat 9, the light collecting lens barrel 8 and the light source seat 9 can be further preferably in threaded connection, and after the light collecting lens barrel 8 is correspondingly connected with the light source seat 9, one side, deviating from the light collecting lens 2, of the annular boss just abuts against the light source 1, so that the light source 1 is positioned, and the light source 1 is prevented from fluctuating along the axial direction. Further preferably, the condenser lens 2 in the preferred embodiment is an aspherical condenser lens; of course, the light collecting mirror 2 may also be optimized in other forms according to actual needs, such as a spherical light collecting lens, which may be optimized according to actual needs, and is not described herein.
Furthermore, double-sided ground glass 3 is coaxially arranged in the light collecting lens barrel 8 corresponding to the light collecting lens 2, and a certain distance is correspondingly formed between the double-sided ground glass and the light collecting lens 2; further preferably, a first spacer 14 is correspondingly arranged between the double-sided frosted glass 3 and the light collecting mirror 2, one end of the first spacer is abutted against the bottom surface of the double-sided frosted glass 3, the other end of the first spacer is abutted against the edge of the light collecting mirror 2, so that the distance between the light collecting mirror 2 and the double-sided frosted glass 3 is constant, and the light collecting mirror 2 can be stably arranged in the light collecting lens barrel 8 through the corresponding action of the annular boss and the first spacer 12; furthermore, a first locking nut 12 is arranged at one end of the light collecting lens barrel 8, which is opposite to the light source seat 9, and can be fixedly connected to the end part of the light collecting lens barrel 8 and abut against the upper end surface of the double-sided ground glass 3 through the end surface, so that the positioning of each part in the light collecting lens barrel 8 is realized, and the stability of the arrangement of the parts is ensured; of course, the middle portions of the first retainer ring 14 and the first lock nut 12 along the axial direction are both hollow, so that light can pass through correspondingly, which can be easily implemented according to related technical means in the prior art, and thus, detailed description is omitted.
Further, the illumination field lens 4 in the preferred embodiment is arranged corresponding to the double-sided ground glass 3, and is preferably provided with a field lens adjusting cylinder 7 corresponding thereto, and then the illumination field lens 4 is coaxially fixed in the field lens adjusting cylinder 7; accordingly, one end of the field lens adjusting cylinder 7 is correspondingly connected to the periphery of the light collecting lens barrel 8, as shown in fig. 1, the matching form of the field lens adjusting cylinder 7 and the light collecting lens barrel 8 is preferably a threaded matching connection, a snap connection, an interference fit connection, or the like. Further preferably, correspond illumination field lens 4 in field lens adjustment barrel 7 and be provided with annular boss along the hoop, illumination field lens 4 corresponds places on annular boss, and correspond and be provided with second space ring 15 in one side that illumination field lens 4 deviates from annular boss, and correspond second space ring 15 and be provided with second lock nut 13 at the tip of field lens adjustment barrel 7, then the both ends of second space ring 15 butt illumination field lens 4 and second lock nut 13 respectively, and second lock nut 13 can correspond the tip of accordant connection at field lens adjustment barrel 7, thereby can realize illumination field lens 4's location and fixed.
Further, the condenser 5 in the preferred embodiment is preferably an abbe condenser, which is coaxially arranged at intervals corresponding to the illumination field lens 4, and a combined lens barrel 6 is arranged between the condenser 5 and the illumination field lens 4, one end of the combined lens barrel 6 is correspondingly connected to the end of the field lens adjusting cylinder 7, the other end of the combined lens barrel 6 is correspondingly connected with the condenser 5, and the combined lens barrel 6 and the condenser 5 are preferably in threaded connection; further, after the combined lens barrel 6 is correspondingly connected with the field lens adjusting cylinder 7, the illumination field lens 4 is arranged at one end of the field lens adjusting cylinder 7 close to the combined lens barrel 6.
Through the arrangement, a light source system consisting of the light source 1, the light collecting lens 2, the double-sided frosted glass 3, the lighting field lens 4 and the collecting lens 5 which are sequentially and coaxially arranged from bottom to top can be vertically formed, the positioning and the fixing of all the components in the light source system can be realized through the structures such as the corresponding lens barrels or the space rings respectively, the structure of the light source system is simple, the arrangement is simple and convenient, and the arrangement stability of the components is high.
Further, when the light source system in the preferred embodiment of the present invention is operated, the imaging device 11 preferably operates corresponding to the light source system, and the imaging device 11 correspondingly performs the imaging of the specimen on the slide 10. The imaging device 11, the slide 10 and the light source system in the preferred embodiment operate correspondingly as shown in fig. 1-3, wherein the objective lens of the imaging device 11 is disposed against the condenser lens 5, and the slide 10 with the specimen loaded and fixed thereon is disposed correspondingly between the imaging device 11 and the condenser lens 5; further preferably, the imaging device in the preferred embodiment comprises an objective lens and a CMOS light-sensitive element, and color imaging of the sample is performed by the CMOS light-sensitive element.
When the light source system and the imaging device 11 in the preferred embodiment of the present invention are used to scan and image the area of the sample on the slide 10, the light emitted by the light source 1 in the light source system acts on the slide 10 through the light path a and the light path B as shown in fig. 2 and 3; the light path a is as shown in fig. 2, light emitted from the light source 1 passes through the double-sided frosted glass 3 after the aperture of the light is reduced by the condenser 2, passes through the illumination field lens 4 and then converges in front of the condenser 5, i.e. the light is converged in the combined lens barrel 6 in an intersecting manner, and then the converged light can be converted into parallel light by the condenser 5 and irradiated onto the glass slide 10, and finally passes through the glass slide 10 and enters the objective lens of the imaging device 11 to be imaged at the entrance pupil of the objective lens; further, the double-sided frosted glass 3 is used as a secondary light source, a pipeline for generating secondary light source imaging as shown in fig. 3 is used as an entrance pupil of the whole illumination light path, the double-sided frosted glass 3 becomes a secondary light source after being illuminated by the light from the light collecting lens 2, then the light on the double-sided frosted glass 3 is irradiated onto the light collecting lens 5 through the aperture reduced after the illumination field lens 4, is further condensed by the light collecting lens 5, and is finally directly imaged on the glass slide 10.
In view of the difference between the optical path a and the optical path B, the final imaging position of the light source 1 is the entrance pupil of the objective lens, and the final imaging position of the double-sided frosted glass 3 is the object plane (i.e. the glass slide 10) of the objective lens, so that an ideal optical energy transfer structure of "pupil-to-pupil and pupil-to-window" is formed between the two optical systems of the illumination light source and the micro-imaging system, thereby fully utilizing the light emitted by the light source 1, improving the illumination brightness of the sample, and avoiding the phenomenon of uneven light source illumination caused by direct imaging of the light source 1 on the sample.
Further preferably, the RGBW four-color LED lamp in the preferred embodiment is used as the light source 1 of the light source system, and the four color lights thereof are not used at the same time in the preferred embodiment, and it preferably works in two forms, the first is: the light source 1 only provides white light, which enables various colors of a sample to be accurately reproduced on the CMOS photosensitive element and forms a color image through one-time imaging; the second method is as follows: the light source 1 provides three monochromatic lights of RGB, and in cooperation with a black-and-white CMOS, the sample is individually focused for each color under the illumination light of the three colors, so as to obtain three monochromatic images, completely eliminate the chromatic aberration of the objective lens itself, improve the imaging quality of the imaging device 11, and then, by giving RGB values to the three monochromatic images, synthesize the three monochromatic images into one color image by adopting a time color mixing manner. The imaging process by the first illumination mode is less in time consumption, and a color image of a sample can be quickly obtained; although the imaging process by the second illumination mode is long, the chromatic aberration of the objective lens can be effectively eliminated, a photo with higher resolution than that of a color CMOS with spatial color mixing is obtained, and the imaging quality of the sample is improved.
The light source system is suitable for high-speed scanning, the light collecting lens 2, the double-sided frosted glass 3, the illumination field lens 4 and the light collecting lens 5 are preferably arranged corresponding to the light source 1 to form a 'double-light-path' light source system, the conditions that the light source in a scanning imaging system is not uniformly irradiated and the sample illumination is insufficient are avoided, the generation of a dazzling phenomenon is reduced, the illumination quality of the sample is improved, and the scanning imaging quality of the sample is further improved.
It will be understood by those skilled in the art that the foregoing is only a preferred embodiment of the present invention, and is not intended to limit the invention, and that any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the scope of the present invention.
Claims (10)
1. A light source system suitable for high-speed scanning is used for completing scanning imaging of a sample by matching with an imaging device (11), and is characterized by comprising a light source (1), a light collecting lens (2), double-sided frosted glass (3), an illumination field lens (4) and a light collecting lens (5) which are coaxially arranged at intervals in sequence; wherein,
the light source (1) is arranged away from the imaging device, arranged on one side of the light collecting mirror (2) and used for generating light rays to irradiate on the light collecting mirror (2);
the light collecting lens (2) is correspondingly arranged between the light source (1) and the double-sided frosted glass (3) and is used for collecting light rays generated by the light source (1) and then transmitting the light rays to the double-sided frosted glass (3) and the illumination field lens (4);
the double-sided frosted glass (3) is arranged between the illumination field lens (4) and the light collecting lens (2), light rays from the light collecting lens (2) can correspondingly penetrate through the double-sided frosted glass (3) and correspondingly irradiate on the illumination field lens (4) to form a first light path, and the double-sided frosted glass (3) can be illuminated and used as a secondary light source (1) to emit light rays onto the illumination field lens (4) to form a second light path;
the illumination field lens (4) is arranged corresponding to the collecting lens (5), so that the light rays of the first light path can be transmitted to the collecting lens (5) after being converged by the illumination field lens (4), and the light rays of the second light path can be correspondingly transmitted to the collecting lens (5) after being deflected by the illumination field lens (4);
condenser lens (5) correspond imaging device (11) sets up, it can with the sample is adjusted well, and will the light of first light path changes the parallel light into and shines on the sample, in order to realize light source (1) is in imaging device's (11) objective entrance pupil department's formation of image, just the light of second light path can pass through converge behind condenser lens (5) sample department, thereby accomplish two-sided ground glass (3) are in the formation of image of sample department.
2. The light source system suitable for high-speed scanning according to claim 1, wherein a light source seat (9) is coaxially arranged corresponding to the light source (1), and the light source (1) can be correspondingly accommodated on the light source seat (9).
3. The light source system suitable for high-speed scanning according to claim 1 or 2, wherein a light collecting barrel (8) is provided corresponding to the light collecting mirror (2), and the light collecting mirror (2) is coaxially fixed in the light collecting barrel (8).
4. The light source system suitable for high-speed scanning according to claim 3, wherein the double-sided frosted glass (3) is coaxially arranged in the light collecting lens barrel (8), a first spacer (14) is arranged between the double-sided frosted glass (3) and the light collecting lens (2), and two ends of the first spacer (14) abut against the double-sided frosted glass (3) and the light collecting lens (2), respectively.
5. The light source system suitable for high-speed scanning according to any one of claims 1 to 4, wherein a field lens adjusting cylinder (7) is arranged corresponding to the illumination field lens (4), and the illumination field lens (4) is coaxially arranged at one end of the field lens adjusting cylinder (7) close to the condenser lens (5).
6. The light source system suitable for high-speed scanning according to claim 5, wherein a combined lens barrel (6) with a hollow interior is arranged between the condenser lens (5) and the illumination field lens (4), the condenser lens (5) is correspondingly fixed at one end of the combined lens barrel (6), and the other end of the combined lens barrel (6) is correspondingly connected with the end of the field lens adjusting barrel (7).
7. The light source system suitable for high-speed scanning according to claim 4, wherein one end of the light collecting lens barrel (8) is provided with a first locking nut (12) in a ring shape corresponding to the double-sided frosted glass (3), the first locking nut can be in threaded connection with the end of the light collecting lens barrel (8), and the end abuts against the double-sided frosted glass (3) so as to realize axial limiting of the double-sided frosted glass (3).
8. The light source system suitable for high-speed scanning according to claim 5, wherein a protrusion is circumferentially arranged on the inner circumferential wall of the field lens adjusting cylinder (7), one side of the field lens (4) is correspondingly abutted to the protrusion, and a second spacer ring (15) and a second lock nut (13) are coaxially arranged on the other side of the field lens (4), the second lock nut (13) can be correspondingly screwed on the end of the field lens adjusting cylinder (7), and two ends of the second spacer ring (15) are respectively abutted to the second lock nut (13) and the field lens (4).
9. A light source system suitable for high speed scanning according to any one of claims 1 to 8, wherein the light source (1) is an RGBW four-color LED lamp.
10. The light source system suitable for high-speed scanning according to any one of claims 1 to 9, wherein the light collecting mirror (2) is an aspherical condenser lens.
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| JP2001154103A (en) * | 1999-11-30 | 2001-06-08 | Mitsutoyo Corp | Illuminator for optical instrument |
| JP2001208977A (en) * | 2000-01-26 | 2001-08-03 | Nikon Corp | Optical microscope |
| CN2833636Y (en) * | 2005-10-18 | 2006-11-01 | 贵阳新天光电科技有限公司 | LED lighting device for measurement microscope |
| JP2008216778A (en) * | 2007-03-06 | 2008-09-18 | Nikon Corp | Structured illuminating microscope |
| CN202631840U (en) * | 2012-06-13 | 2012-12-26 | 南京东利来光电实业有限责任公司 | Small inverted biological microscope |
| CN103197413A (en) * | 2013-04-15 | 2013-07-10 | 厦门大学 | Optics microscopic imaging method capable of generating three-dimensional images |
| CN107076974A (en) * | 2014-10-03 | 2017-08-18 | 奥林巴斯株式会社 | Optical axis direction scan microscope device |
| CN208521058U (en) * | 2018-08-08 | 2019-02-19 | 杭州上池科技有限公司 | A kind of light-source system suitable for high-velocity scanning |
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| CN109031637B (en) | 2024-01-05 |
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