CN109012770A - Multi-ply paper chip structure, manufacturing equipment and method and fluid interlayer current method - Google Patents
Multi-ply paper chip structure, manufacturing equipment and method and fluid interlayer current method Download PDFInfo
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- CN109012770A CN109012770A CN201810764200.2A CN201810764200A CN109012770A CN 109012770 A CN109012770 A CN 109012770A CN 201810764200 A CN201810764200 A CN 201810764200A CN 109012770 A CN109012770 A CN 109012770A
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502707—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the manufacture of the container or its components
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/50273—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the means or forces applied to move the fluids
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/10—Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0887—Laminated structure
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/12—Specific details about materials
- B01L2300/126—Paper
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
- B01L2400/0415—Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
- B01L2400/0487—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics
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- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Hematology (AREA)
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- Automatic Analysis And Handling Materials Therefor (AREA)
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Abstract
The invention discloses multi-ply paper chip structures, including at least two layers of paper base material, and paper base material is resin layer at least one fluid space formed therewith between layers；The fluid space includes sample introduction zone, sample introduction zone connection fluid channel, at least one detection zone of fluid channel connection；The sample introduction zone of paper base material is on the same straight line perpendicular to paper base material.The invention also provides the manufacturing equipment of multilayer paper chip, manufacturing method and the methods for controlling fluid interflow.The invention has the advantages that the bioanalysis of complicated multistep on paper may be implemented in the multilayered structure of the multilayer paper chip；Fluid channel is not filled by cellulose, realizes the isolation of interlayer, and outside applies electric field or airflow function just realizes the interflow of fluid, and detection process is made to have controllability；The manufacturing method realizes process automation, realizes the automatic bonding between different layers paper and alignment；The fluid interlayer current method has used electric field or air-flow to control, so that control process sequencing and automation.
Description
Technical field
The present invention relates to the structures of paper chip, method of manufacture and use thereof field, more particularly, to multi-ply paper chip structure, system
Manufacturing apparatus and method and fluid interlayer current method.
Background technique
Paper chip microfluidic device is to be put forward for the first time by Whitesides study group of Harvard University in 2007, specifically
It is that traditional micro-fluidic substrates such as glass, silicon, high polymer are replaced with paper, processes flow path on the surface of paper, it is solid by paper
Some capillary forces realize the transport and transfer of liquid, have many advantages, such as that light, at low cost, easy acquisition, bio-compatibility are good.Root
According to flow direction, paper chip microfluid can be divided into two-dimentional paper chip and three-dimensional paper chip, and the preparation method of two-dimentional paper chip includes
Wax printing, inkjet printing, photoetching, plasma etching etc..Compared to two-dimentional paper chip, three-dimensional paper chip is in measurement capacity, equipment
Complexity and functional aspect be significantly improved.The it is proposeds such as Santhiago are prepared with the paper that adhered by double sided plaster is folded
Three-dimensional paper chip electrochemistry detecting apparatus, although equipment is fairly simple, there is still a need for accurately manual in preparation process
Alignment.Whitesides etc. is printed on the paper of different pattern with sticking double faced adhesive tape multilayer, punches then on double-sided adhesive designated position
Cellulose is filled out to allow solution to be penetrated into next layer from upper one layer；Complex manufacturing process and precision requirement height, and load
Cellulose can have suction-operated to molecule.Later, Crooks etc. proposed paper folding method, and the mirror image that paper folding method is related to paper folding is asked
Topic folds precision and requires height, while also needing auxiliary device so that neighbouring two layers of close contact.The it is proposeds such as Lewis are used
Wax printing technology combination glue spraying prepares multilayer paper chip, although the method is efficiently, operates more demanding to experimenter and sprays
Wax printer is sufficiently expensive.Reporting there is presently no research can integration and automation preparation multilayer paper chip microfluidic device
Method.
The control method that liquid flows in paper chip is broadly divided into three classes.Most straightforward approach is setting length or width
Different flow paths, flowing time extend with the increase of path length or width, and this method flows liquid
Ability of regulation and control is limited, if wanting to keep carryover effects obvious, the volume of equipment can also be increased accordingly.Secondly change paper with chemical reagent
The wettability for opening surface is to control the second class method of liquid flowing comprising sugar, paraffin, surfactant, alkyl ketene two
Aggressiveness, gel etc.；Hydrophilic pathway is engraved on paper with the conventional method for preparing single-part stationery microfluidic device first, then again
Channel is handled with chemical reagent, the flowing velocity of liquid on a passage can subtract with the increase of chemical agent concentration
It is slow.Paper chip this method increase the preparation step of paper chip and by chemical reagent processing may be to certain compounds
Detection have interference.Third class method is the flowing of liquid to be controlled by mechanical mode, such as control the electricity of channel switch
Magnet, the triboelectric effect for accelerating liquid flowing, the transfer limitation dissolvable bridge of liquid, the water imbibition sponge of separating liquid, even
Connect the movable valve etc. of flow path；The above method primarily directed on single-part stationery liquid flow control, equipment preparation process and
Liquid manipulation process is relative complex.So being able to achieve the on-off control of liquid in single layer paper chip at present, multilayer paper chip is to liquid
The on-off of body only has delays time to control, but the liquid in multilayer paper chip can not carry out on-off control.
Summary of the invention
To solve the above-mentioned problems, the invention proposes multi-ply paper chip structure, provide it is a kind of can simple, integration and from
The method of dynamicization preparation multilayer paper chip；The manufacturing equipment and manufacturing method of the multilayer paper chip are proposed simultaneously, the equipment knot
Structure is simple, and multi-ply paper automatic bonding and alignment during printing avoid the use of viscose glue, reduce human error.Together
When also proposed control microfluid in the method for paper chip interflow, biology of this method especially suitable for multistep complicated on paper
Analysis, such as Enzyme-linked Immunosorbent Assay, signal expand immune response, detection of nucleic acids etc.；Using Arduino software by electric field or gas
The control program of stream is finished, and allows one layer of liquid according to the time of setting successively toward flowing down, such multistep complex reaction can
With the progress of automation, reduce artificial participation, the accuracy and repeatability of multistep reaction can be improved.
To achieve the goals above, the present invention adopts the following technical scheme: multi-ply paper chip structure, including at least two layers of paper
Substrate, paper base material are resin layer at least one fluid space formed therewith between layers；The fluid space includes sample introduction
Area, sample introduction zone connection fluid channel, at least one detection zone of fluid channel connection；The sample introduction zone of same fluid space perpendicular to
On the same straight line of paper base material.
Further, the multi-ply paper chip structure further includes, top side paper base material surface there are also basal layer, basal layer by
Resin prints to be formed.
Further, the material of the paper base material is filter paper.
Further, the multi-ply paper chip manufacturing apparatus, including light source, light transmission device, reaction vessel and platform；Wherein
Light transmission device, for realizing the shape with change lit transmissive region；Light transmission device is in the top of light source；Reaction vessel,
Fill resin liquid；Light transmission device is mounted on reaction vessel bottom surface, and light source can be transmitted through light transmission device into reaction vessel；Platform,
Can vertical up-or-down movement, in printing paper chip processes place paper chip semifinished or finished goods；Platform is in setting most
When lower end position, thickness of the distance formed between reaction vessel bottom surface as resin coagulation.
Further, the light source is LED light.
Further, the light transmission device is LCD flat panel, is used to receive and show the transmission region of Computer Design
Shape, light source can only pass through transmission region and enter reaction vessel.
Further, the multi-ply paper manufacturing method of chip, comprising steps of
S1, light transmission device from the shape of the transmission region that downloaded designs, platform drop to itself and reaction vessel bottom surface it
Between distance be setting base layer thickness；Light source is opened, is exposed by setting time, blank substrate layer is printed；Print blank base
After bottom, light source is closed, platform rises, and basal layer is adhered to platform lower surface；
S2, it is put into paper base material in the reaction vessel, platform drops to setting position, opens light source, exposes by setting time, printing
First layer resin layer；After printing this layer of resin layer, light source is closed, platform rises, and paper chip semi-finished product are adhered under platform
Surface；
S3, it is put into paper base material in the reaction vessel, light transmission device is under the shape of the transmission region that downloaded designs, platform
It is down to setting position, opens light source, is exposed by setting time, prints this layer of resin layer；After printing this layer of resin layer, close
Light source, platform rise, and paper chip semi-finished product are still adhered to platform lower surface；
S4, S3 is repeated until completing the printing of all resin layers.
Further, the multi-ply paper chip fluid interflow method, comprising: dripped in the outermost sample introduction zone of paper chip
Enter sample to be tested, apply electric field in the detection zone two sides of paper chip or applies air-flow in the detection zone unilateral side of paper chip.
Compared with prior art, the beneficial effects of the present invention are:
1. bioanalysis of the multilayered structure of the multilayer paper chip especially suitable for complicated multistep, such as Enzyme-linked Immunosorbent Assay, letter
Number expand immune response, detection of nucleic acids etc.；
2. the fluid channel of the multilayer paper chip is not filled by cellulose, realize the isolation of interlayer, only when outside apply electric field or
Person's airflow function just realizes the interflow of fluid, and detection process is made to have controllability；
3. the manufacturing method realizes process automation, the automatic bonding between different layers paper and alignment are realized, viscose glue is avoided
Use, reduce human error；
4. the manufacturing method carries out photocuring using digitlization exposure mask, simple and convenient, it is easy to standardize production；After the completion of printing,
Uncured resin is cleaned up dry；
5. the fluid interlayer current method has used electric field or air-flow to control, so that control process sequencing and automation,
The control program of electric field or air-flow is finished using Arduino software, allow one layer of liquid according to setting time successively
It is automatic past dirty, reduce artificial participation, the accuracy and repeatability of multistep reaction can be improved.
Detailed description of the invention
Fig. 1 is the paper chip structural schematic diagram of the embodiment of the present invention one；
Fig. 2 is the portion the paper chip A diagrammatic cross-section of the embodiment of the present invention one；
Fig. 3 is the paper chip schematic top plan view of the embodiment of the present invention one；
Fig. 4 is the paper chip manufacturing equipment structural schematic diagram of the embodiment of the present invention one；
Fig. 5 is pictorial diagram of the paper chip melted ink of the embodiment of the present invention one after fluid channel；
Fig. 6 is the experiment effect figure before and after application electric field after the melted ink of the embodiment of the present invention two；
Fig. 7 is the experiment effect figure before and after application air-flow after the melted ink of the embodiment of the present invention three；
Description of symbols: 1- paper base material；2- sample introduction zone；3- resin layer；4- basal layer；5- fluid space；51- detection zone；52-
Fluid channel；6- sample to be tested；7- light source；8- light transmission device；9- reaction vessel；10- resin liquid；11- platform.
Specific embodiment
For a clearer understanding of the technical characteristics, objects and effects of the present invention, this hair of Detailed description of the invention is now compareed
Bright specific embodiment, but protection scope of the present invention be not limited to it is as described below.
Specific embodiment one:
As shown in Figure 1 to Figure 3, the present invention provides multi-ply paper chip structures, including at least two layers of paper base material 1,1 layer of paper base material with
It is at least one fluid space 5 formed therewith of resin layer 3 between layer；The fluid space 5 includes sample introduction zone 2, and sample introduction zone 2 connects
Fluid channel 52 is connect, fluid channel 52 connects at least one detection zone 51；The sample introduction zone 2 of the same fluid space 5 of paper base material 1 exists
Perpendicular on the same straight line of paper base material 1.The multi-ply paper chip structure further includes that there are also bases on top side paper base material 1 surface
Bottom 4, basal layer 4 is printed by resin to be formed.The material of the paper base material 1 is filter paper.Paper base material 1 is cellulose, and fluid is in list
Layer paper base material 1 can diffuse to detection zone 51 along fluid channel 52, but the fluid of the sample introduction zone 2 of certain layer of paper base material 1 cannot
Cross-layer gets over the sample introduction zone 2 to next door layer, so sample to be tested 6 is spread without only will do it single layer in the case of external force.The work of basal layer 4
With being to make up manufacturing device there may be unhorizontal situations, directly paper base material 1 is printed, is inclined if paper base material 1 is in
In the case where tiltedly, 3 uneven thickness of resin layer that may print, it is possible to which most thin place does not just have resin solidification；Substrate
4 essence of layer is also one layer of resin layer, but this resin layer does not do fluid channel use, is only that its lower surface is made to be horizontal, that
Paper base material 1 is placed on the basis of basal layer 4 afterwards is almost just disposed on horizontal plane.Detection zone 51 can be infused in advance in practical applications
Enter the substance reacted with sample to be tested 6, which can trigger the variation that can intuitively watch.The material of paper base material 1 is filter paper, can
With inspiration, the filter paper material is also alternatively at the filter paper, chromatographic paper, filter membrane and ion exchange paper of close effect therewith.
The sample introduction zone of paper base material 1 is actually to run through the hole of resin layer 3, and the position of this some holes is when designing core chip architecture
It has been determined that this some holes is point-blank, in order to allow sample to be tested 6 in top layer the external world apply electric field force or
It can be penetrated through under person's airflow function and flow to all layers of paper base material 1, once certain layer of paper base material 1 has sample to be tested 6 in sample introduction zone
2, which can be diffused under the effect of the cellulose of paper base material 1 along fluid channel 52, and go directly detection zone 51,
That is it drips in the positive liquid of paper base material 1, can also diffuse to the reverse side of paper base material 1；Conversely, paper base material 1 is stained with resin
Place then diffusion be terminated, fluid only no resin adherency fluid channel 52 spread.The characteristics of paper chip first is that
Possess at least one fluid space 5, each fluid space 5 has respective sample introduction zone 2, is especially suitable for applying in complicated multi-step
Bioanalysis, for example Enzyme-linked Immunosorbent Assay, signal expand immune response, detection of nucleic acids etc.；After sample area 2 instills sample to be tested 6,
The control program of electric field or air-flow is finished using Arduino software, sample to be tested 6 is according to the time of setting successively from sample introduction
Area 2 flows downstream into fluid channel 52, and then flow detection area 51 automatically.
As shown in figure 4, multi-ply paper chip manufacturing apparatus, it is applied to manufacture the multi-ply paper chip structure, including light source 7, thoroughly
Electro-optical device 8, reaction vessel 9 and platform 11；Wherein, light transmission device 8, for realizing the shape with change 7 transmission region of light source；
Light transmission device 8 is in the top of light source 7；Reaction vessel 9 fills resin liquid 10；Light transmission device 8 is mounted on 9 bottom surface of reaction vessel,
Light source 7 can be transmitted through light transmission device 8 into reaction vessel 9；Platform 11, can vertical up-or-down movement, be used for printing paper chip processes
The middle semifinished or finished goods for placing paper chip；When platform 11 is in the lowest positions of setting, between 9 bottom surface of reaction vessel
Thickness of the distance of formation as resin coagulation.The light source 7 is LED light.The light transmission device 8 is LCD flat panel, is used to connect
Receive and show the shape of the transmission region of Computer Design, light source 7 can only pass through transmission region and enter reaction vessel 9.Herein
Resin liquid 10 is light-cured resin liquid, can under the irradiation of the light of special wavelength by the photosensitizer initiated polymerization inside it,
Physical and chemical changes can occur rapidly in a relatively short period of time, and then crosslinking curing generates insoluble film, irradiation time is got over
Its long coating thickness is bigger, and it is the blue light of 405 nm that the present embodiment, which uses light source,.The pattern of light transmission device 8 divides dark and light area,
What the light of dark areas was impermeable, the light in area pellucida domain be it is permeable, dark, the area pellucida domain of light transmission device are shifted to an earlier date according to personnel
It is shown in the pattern of Computer Design.There is masking foil in the lower surface of platform 11, and resin liquid 10 solidifies the film adherency generated
In platform lower surface, irradiation time can determine that the hardness of the resin printed, hardness influence whether resin and last layer
Adhesion strength.
Multi-ply paper manufacturing method of chip manufactures multi-ply paper chip structure applied to multi-ply paper manufacturing method of chip, tree
Total number of plies of rouge layer 3 be three layers (do not include basal layer 4, behind the number of plies counting similarly), comprising steps of
From the shape of the transmission region that downloaded designs, platform 11 drops to itself and 9 bottom of reaction vessel for S1, light transmission device 8
The distance between face is 4 thickness of basal layer of setting；Light source 7 is opened, 80s is exposed, prints blank substrate layer 4；Print blank base
After bottom 4, light source 7 is closed, platform 11 rises, and basal layer 4 is adhered to 11 lower surface of platform；
S2, it is put into paper base material 1 in reaction vessel 9, platform 11 drops to setting position, opens light source 7, exposes 80s, printing the
One layer of resin layer 3；After printing this layer of resin layer 3, light source 7 is closed, platform 11 rises, and paper chip semi-finished product are adhered to platform
11 lower surfaces；
S3, paper base material 1 is put into reaction vessel 9, light transmission device 8 is put down from the shape for the transmission region that downloaded designs
Platform 11 drops to setting position, opens light source 7, exposes 55s, prints this layer of resin layer 3；After printing this layer of resin layer 3, close
Black out source 7, platform 11 rise, and paper chip semi-finished product are still adhered to 11 lower surface of platform；
S4, S3 is repeated until completing the printing of third layer resin layer 3, wherein the time for exposure is 55s.
As shown in figure 5, being pictorial diagram of the paper chip melted ink of the embodiment after fluid channel.
It should be noted that light can penetrate paper chip 1, it is cured in the upper and lower surface two sides solidified resin of paper chip 1
Resin can be adhered to formation resin layer 3 in paper chip.In addition, the resin having cured in print procedure is adhered in the flat of aluminium alloy
11 lower surface of platform, when printed substrates layer 4, the adhesive effect of resin and platform 11 is not very strong, therefore the time for exposure just needs to grow
Some adherency for enhancing the two with this.After basal layer 4 has printed, when printing the second layer, the cured tree in 1 upper surface of paper chip
Rouge layer 3 adheres on basal layer 4, and the two adherency is compared with the former more more easily, therefore the time for exposure of basal layer 4 can be arranged
It obtains slightly more longer than other resin layers 3.
Specific embodiment two:
On the basis of example 1, multi-ply paper manufacturing method of chip is used to manufacture two layers of resin layer 3, that is, has two layers of paper base
Material 1,4 time for exposure of basal layer 80s, first layer and second layer resin layer time for exposure are respectively 80s and 55s.
Multi-ply paper chip fluid interflow method, the paper chip manufactured applied to the present embodiment, comprising: in core
The outermost sample introduction zone 2 of piece instills sample to be tested 6, applies electric field in 51 two sides of detection zone of paper chip.As shown in fig. 6, figure is left
Be embodiment two paper chip melted ink after apply electric field before front picture, figure right side be application electric field after paper chip the back side
Picture；It should be noted that the ink of the sample introduction zone 2 of positive top layer is cured, resin is limited to the region, makees by electric field
With rear, ink extends through second layer paper base material 1, can more clearly from see that ink is through circulation passage from the back side of paper chip
52 diffuse to detection zone 1.
Specific embodiment three:
On the basis of example 2, the fluid interlayer current method for changing control paper chip, instead applies application electric field action
Add airflow function.Apply the front picture before air-flow after scheming the paper chip melted ink that a left side is embodiment two, the figure right side is to apply air-flow
The back side picture of paper chip afterwards；It should be noted that the ink of the sample introduction zone 2 of positive top layer is cured, resin is limited to this
Region, after airflow function, ink extends through second layer paper base material 1, can more clearly from see from the back side of paper chip,
Ink diffuses to detection zone 1 through circulation passage 52.It can inspire, air-flow is the one kind for belonging to mechanical force, and the mechanical force is also
It can be replaced with similar mechanical force, such as directly press sample introduction zone 2, the sample to be tested 6 of sample introduction zone 2 crosses due directly to squeezing
Next layer.
It can be concluded that the multilayered structure of the multilayer paper chip is particularly suitable for the bioanalysis of complicated multistep on paper, than
Such as Enzyme-linked Immunosorbent Assay, signal expand immune response, detection of nucleic acids；The fluid channel of the multilayer paper chip is not filled by fiber
Element, realizes the isolation of interlayer, only applies electric field or airflow function just realizes the interflow of fluid when external, makes to detect
Journey has controllability；The manufacturing method realizes process automation, realizes the automatic bonding between different layers paper and alignment, avoids
The use of viscose glue, reduces human error；The manufacturing method carries out photocuring using digitlization exposure mask, simple and convenient, is easy to
Standardization production；After the completion of printing, uncured resin is cleaned up dry；The fluid interlayer current method uses
Electric field or air-flow control, so that control process sequencing and automation, using Arduino software by electric field or air-flow
Control program is finished, and is made one layer of liquid successively automatic toward flowing down according to the time of setting, is reduced artificial participation, Ke Yiti
The accuracy and repeatability of high multistep reaction.
Disclosed above is only presently preferred embodiments of the present invention, cannot limit the right model of the present invention with this certainly
It encloses, therefore makees ground equivalent variations according to the claims in the present invention, be still within the scope of the present invention.
Claims (8)
1. multi-ply paper chip structure, which is characterized in that including at least two layers of paper base material (1), paper base material (1) is tree between layers
Rouge layer (3) at least one fluid space (5) formed therewith；The fluid space (5) includes sample introduction zone (2), and sample introduction zone (2) is even
It connects fluid channel (52), fluid channel (52) connects at least one detection zone (51)；The sample introduction zone (2) of same fluid space (5)
On the same straight line perpendicular to paper base material (1).
2. multi-ply paper chip structure as described in claim 1, it is characterised in that: the multi-ply paper chip structure further includes, most
There are also basal layer (4), basal layer (4) is printed by resin to be formed on upside paper base material (1) surface.
3. multi-ply paper chip structure as described in claim 1, it is characterised in that: the material of the paper base material (1) is filter paper.
4. multi-ply paper chip manufacturing apparatus, the manufacturing equipment is applied to described in manufacturing claims 1 to any one of claim 3
Multi-ply paper chip structure characterized by comprising
Light source (7), and
Light transmission device (8), for realizing the shape with change light source (7) transmission region；Light transmission device (8) is in light source (7)
Top；
Reaction vessel (9) fills resin liquid (10)；Light transmission device (8) is mounted on reaction vessel (9) bottom surface, and light source (7) can be through
Light transmission device (8) is transmitted into reaction vessel (9)；
Platform (11), can vertical up-or-down movement, in printing paper chip processes place paper chip semifinished or finished goods；It is flat
When platform (11) is in the lowest positions of setting, the distance formed between reaction vessel (9) bottom surface is as resin coagulation
Thickness.
5. multi-ply paper chip manufacturing apparatus as claimed in claim 4, it is characterised in that: the light source (7) is LED light.
6. multi-ply paper chip manufacturing apparatus as claimed in claim 4, it is characterised in that: the light transmission device (8) is LCD flat
Plate, is used to receive and show the shape of the transmission region of Computer Design, and light source (7) can only pass through transmission region and enter reaction
Container (9).
7. multi-ply paper manufacturing method of chip, the manufacturing method is applied to multi-ply paper chip manufacturing apparatus as claimed in claim 4,
Characterized in that it comprises the following steps:
From the shape of the transmission region that downloaded designs, platform (11) drops to itself and reaction vessel for S1, light transmission device (8)
(9) the distance between bottom surface is basal layer (4) thickness of setting；It opens light source (7), is exposed by setting time, print blank base
Bottom (4)；It after printing blank substrate layer (4), closes light source (7), platform (11) rises, and basal layer (4) is adhered to platform
(11) lower surface；
S2, paper base material (1) is put into reaction vessel (9), platform (11) drops to setting position, opens light source (7), by setting
Time Exposure prints first layer resin layer (3)；It after printing this layer of resin layer (3), closes light source (7), on platform (11)
It rises, paper chip semi-finished product are adhered to platform (11) lower surface；
S3, paper base material (1) is put into reaction vessel (9), shape of the light transmission device (8) from the transmission region that downloaded designs
Shape, platform (11) drop to setting position, open light source (7), expose by setting time, print this layer of resin layer (3)；Printing should
It after layer resin layer (3), closes light source (7), platform (11) rises, and paper chip semi-finished product are still adhered to platform (11) following table
Face；
S4, S3 is repeated until completing the printing of all resin layers (3).
8. multi-ply paper chip fluid interflow method, the current method is applied to claim 1 to any one of claim 3
The multi-ply paper chip structure characterized by comprising instill sample to be tested in the outermost sample introduction zone (2) of paper chip
(6), apply electric field in the detection zone of paper chip (51) two sides or apply air-flow in detection zone (51) unilateral side of paper chip.
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| CN201810764200.2A CN109012770B (en) | 2018-07-12 | 2018-07-12 | Multilayer paper chip structure, manufacturing apparatus and method, and fluid interlayer flow method |
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| CN109012770B CN109012770B (en) | 2021-05-14 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN118322551A (en) * | 2024-04-25 | 2024-07-12 | 南京航空航天大学 | Rapid preparation method of multilayer microfluidic chip based on photo-curing molding technology |
Citations (23)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| TW593128B (en) * | 2002-05-17 | 2004-06-21 | Fan-Gen Tzeng | Method for manufacturing three-dimensional microstructure |
| CN102016595A (en) * | 2008-03-27 | 2011-04-13 | 哈佛学院院长等 | Three-dimensional microfluidic devices |
| CN102914536A (en) * | 2012-10-19 | 2013-02-06 | 大连大学 | Patterned multilayer array paper chip, preparation method and application thereof |
| CN102998439A (en) * | 2011-09-14 | 2013-03-27 | 佳木斯大学 | Micro-fluidic paper chip for simultaneously detecting glucose, uric acid, triglycerides and cholesterols, and its manufacturing method |
| CN103055967A (en) * | 2012-12-27 | 2013-04-24 | 济南大学 | A preparation method of a simple, low-cost, multi-channel microfluidic chemiluminescent paper chip and an on-site detection method by using the same |
| CN103722745A (en) * | 2013-12-29 | 2014-04-16 | 北京工业大学 | Quick resin forming method based on LCD (liquid crystal display) selective regional light transmission principle |
| CN104069904A (en) * | 2014-07-16 | 2014-10-01 | 华南师范大学 | Preparation method for ultraviolet photoetching technique-based cloth micro fluidic chip |
| US20140349279A1 (en) * | 2011-12-15 | 2014-11-27 | Commissariat à l'énergie atomique et aux énergies alternatives | 3d microfluidic system having nested areas and a built-in reservoir, method for the preparing same, and uses thereof |
| CN104339653A (en) * | 2013-08-05 | 2015-02-11 | 李家萌 | Light curing 3D printing device and printing method |
| CN104475178A (en) * | 2014-12-01 | 2015-04-01 | 浙江大学 | Micro-fluidic paper chip manufacturing device and micro-fluidic paper chip manufacturing method |
| CN105903502A (en) * | 2016-05-16 | 2016-08-31 | 南京工业大学 | Preparation method of micro-fluidic paper chip based on heat transfer wax hydrophilic and hydrophobic pattern |
| CN104841499B (en) * | 2015-04-24 | 2016-09-28 | 复旦大学 | A kind of paper substrate numeral microfluidic device |
| CN106732840A (en) * | 2017-01-24 | 2017-05-31 | 厦门大学 | The 3D printing method and device of nanofiber paper substrate layered manufacturing micro-fluidic chip |
| WO2017089963A1 (en) * | 2015-11-23 | 2017-06-01 | King Abdullah University Of Science And Technology | Methods of making microfluidic devices |
| US20170173578A1 (en) * | 2014-03-14 | 2017-06-22 | Board Of Regents, The University Of Texas System | Microfluidic devices for the rapid detection of analytes |
| KR20170078057A (en) * | 2015-12-29 | 2017-07-07 | 광주과학기술원 | Chip structure for multiple molecular diagonosis |
| CN106985382A (en) * | 2017-04-25 | 2017-07-28 | 上海联泰科技股份有限公司 | 3D printing method and the printing device being applicable |
| CN107478631A (en) * | 2017-09-19 | 2017-12-15 | 南京工业大学 | 3D folding paper base microfluid fluorescence detection device capable of simultaneously detecting multiple tumor markers |
| CN107734862A (en) * | 2017-11-30 | 2018-02-23 | 济南大学 | A kind of construction method of three-dimensional paper substrate multifunctional circuit |
| CN107899626A (en) * | 2017-12-05 | 2018-04-13 | 哈尔滨工业大学 | A kind of three-dimensional paper chip based on thin double faced adhesive tape and lamination techniques and preparation method thereof |
| CN107991484A (en) * | 2017-11-20 | 2018-05-04 | 中国科学院烟台海岸带研究所 | A kind of rotary electrical chemo-immunity refill card analyzer and its detection method |
| CN108020585A (en) * | 2017-12-05 | 2018-05-11 | 哈尔滨工业大学 | A kind of three-dimensional paper chip of integrated colour developing and Electrochemical Detection and preparation method thereof |
| CN108088841A (en) * | 2017-12-15 | 2018-05-29 | 长春理工大学 | Paper substrate micro-fluidic chip that is a kind of while detecting four kinds of liver function enzymes and preparation method thereof |
-
2018
- 2018-07-12 CN CN201810764200.2A patent/CN109012770B/en active Active
Patent Citations (23)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| TW593128B (en) * | 2002-05-17 | 2004-06-21 | Fan-Gen Tzeng | Method for manufacturing three-dimensional microstructure |
| CN102016595A (en) * | 2008-03-27 | 2011-04-13 | 哈佛学院院长等 | Three-dimensional microfluidic devices |
| CN102998439A (en) * | 2011-09-14 | 2013-03-27 | 佳木斯大学 | Micro-fluidic paper chip for simultaneously detecting glucose, uric acid, triglycerides and cholesterols, and its manufacturing method |
| US20140349279A1 (en) * | 2011-12-15 | 2014-11-27 | Commissariat à l'énergie atomique et aux énergies alternatives | 3d microfluidic system having nested areas and a built-in reservoir, method for the preparing same, and uses thereof |
| CN102914536A (en) * | 2012-10-19 | 2013-02-06 | 大连大学 | Patterned multilayer array paper chip, preparation method and application thereof |
| CN103055967A (en) * | 2012-12-27 | 2013-04-24 | 济南大学 | A preparation method of a simple, low-cost, multi-channel microfluidic chemiluminescent paper chip and an on-site detection method by using the same |
| CN104339653A (en) * | 2013-08-05 | 2015-02-11 | 李家萌 | Light curing 3D printing device and printing method |
| CN103722745A (en) * | 2013-12-29 | 2014-04-16 | 北京工业大学 | Quick resin forming method based on LCD (liquid crystal display) selective regional light transmission principle |
| US20170173578A1 (en) * | 2014-03-14 | 2017-06-22 | Board Of Regents, The University Of Texas System | Microfluidic devices for the rapid detection of analytes |
| CN104069904A (en) * | 2014-07-16 | 2014-10-01 | 华南师范大学 | Preparation method for ultraviolet photoetching technique-based cloth micro fluidic chip |
| CN104475178A (en) * | 2014-12-01 | 2015-04-01 | 浙江大学 | Micro-fluidic paper chip manufacturing device and micro-fluidic paper chip manufacturing method |
| CN104841499B (en) * | 2015-04-24 | 2016-09-28 | 复旦大学 | A kind of paper substrate numeral microfluidic device |
| WO2017089963A1 (en) * | 2015-11-23 | 2017-06-01 | King Abdullah University Of Science And Technology | Methods of making microfluidic devices |
| KR20170078057A (en) * | 2015-12-29 | 2017-07-07 | 광주과학기술원 | Chip structure for multiple molecular diagonosis |
| CN105903502A (en) * | 2016-05-16 | 2016-08-31 | 南京工业大学 | Preparation method of micro-fluidic paper chip based on heat transfer wax hydrophilic and hydrophobic pattern |
| CN106732840A (en) * | 2017-01-24 | 2017-05-31 | 厦门大学 | The 3D printing method and device of nanofiber paper substrate layered manufacturing micro-fluidic chip |
| CN106985382A (en) * | 2017-04-25 | 2017-07-28 | 上海联泰科技股份有限公司 | 3D printing method and the printing device being applicable |
| CN107478631A (en) * | 2017-09-19 | 2017-12-15 | 南京工业大学 | 3D folding paper base microfluid fluorescence detection device capable of simultaneously detecting multiple tumor markers |
| CN107991484A (en) * | 2017-11-20 | 2018-05-04 | 中国科学院烟台海岸带研究所 | A kind of rotary electrical chemo-immunity refill card analyzer and its detection method |
| CN107734862A (en) * | 2017-11-30 | 2018-02-23 | 济南大学 | A kind of construction method of three-dimensional paper substrate multifunctional circuit |
| CN107899626A (en) * | 2017-12-05 | 2018-04-13 | 哈尔滨工业大学 | A kind of three-dimensional paper chip based on thin double faced adhesive tape and lamination techniques and preparation method thereof |
| CN108020585A (en) * | 2017-12-05 | 2018-05-11 | 哈尔滨工业大学 | A kind of three-dimensional paper chip of integrated colour developing and Electrochemical Detection and preparation method thereof |
| CN108088841A (en) * | 2017-12-15 | 2018-05-29 | 长春理工大学 | Paper substrate micro-fluidic chip that is a kind of while detecting four kinds of liver function enzymes and preparation method thereof |
Non-Patent Citations (4)
| Title |
|---|
| CHANYONG PARK: "Double-sided 3D printing on paper towards mass production of three-dimensional paper-based microfluidic analytical devices (3D-μPADs)", 《LAB ON A CHIP》 * |
| LEI LIU: "Sensitive colorimetric detection of Cu2+ by simultaneous reaction and electrokinetic stacking on a paper-based analytical device", 《MICROCHEMICAL JOURNAL》 * |
| YONG HE: "Rapid fabrication of paper-based microfluidic analytical devices with desktop stereolithography 3D printer", 《RSC ADVANVES》 * |
| 王虎: "三维纸质微流控芯片的设计制作及其在重金属检测中的应用", 《中国优秀硕士学位论文全文数据库 工程科技Ⅰ辑》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN118322551A (en) * | 2024-04-25 | 2024-07-12 | 南京航空航天大学 | Rapid preparation method of multilayer microfluidic chip based on photo-curing molding technology |
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