CN109010898A - A kind of chitosan-based gauze dressing of loaded metal ion and preparation method thereof - Google Patents
A kind of chitosan-based gauze dressing of loaded metal ion and preparation method thereof Download PDFInfo
- Publication number
- CN109010898A CN109010898A CN201811115484.9A CN201811115484A CN109010898A CN 109010898 A CN109010898 A CN 109010898A CN 201811115484 A CN201811115484 A CN 201811115484A CN 109010898 A CN109010898 A CN 109010898A
- Authority
- CN
- China
- Prior art keywords
- metal ion
- chitosan
- gauze dressing
- loaded
- solution
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/22—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons containing macromolecular materials
- A61L15/28—Polysaccharides or their derivatives
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/18—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons containing inorganic materials
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/42—Use of materials characterised by their function or physical properties
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/42—Use of materials characterised by their function or physical properties
- A61L15/44—Medicaments
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/10—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices containing or releasing inorganic materials
- A61L2300/102—Metals or metal compounds, e.g. salts such as bicarbonates, carbonates, oxides, zeolites, silicates
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
- A61L2300/412—Tissue-regenerating or healing or proliferative agents
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Materials Engineering (AREA)
- Epidemiology (AREA)
- Hematology (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Inorganic Chemistry (AREA)
- Materials For Medical Uses (AREA)
Abstract
It is an object of the invention to provide a kind of chitosan-based gauze dressing of loaded metal ion, the metal ion of gauze dressing load can be added as needed one or more, may be directly applied to the surface of a wound, can also be by applying after stacking layer by layer.The concentration of metal ions of load can be adjusted by changing the metal ion solution concentration prepared.
Description
Technical field
The present invention relates to a kind of chitosan-based gauze dressings and preparation method thereof for loading multi-metal ion, can be used as urgency
The property surface of a wound or infective wound surface dressing.
Background technique
Metal ion is the essential microelement of human body, and the research in the surface of a wound at present and application are increasing.Perhaps
Mostly research shows that Ag+, Ca2+, Cu2+, Zn2+, Mg2+, Fe3+Plasma each contributes to infective wound surface reparation.However, metal ion is general
Reach slow releasing function all over being more toxic for biology, therefore using chitosan and metal ion crosslinked plastic, to reduce
Toxicity and wound healing.Chitosan (CS) is due to its nontoxicity, and biological degradability, biocompatibility can be by bio-absorbable
Etc. characteristics, be widely used in biomedical applications.There is supermolecule complexing between natural CS chain and each metal ion species
Crosslinked action.It only needs under pH value appropriate, at room temperature mixes metal ion solution with chitosan solution, the two is sent out
Raw very quickly complexing, ultimately forms hydrogel.
In addition, chitosan solution is mixed with metal ion solution, add a certain proportion of NaOH solution, can moment at
Glue.However have a disadvantage in that formed glue is softer more broken, it can not be directly made into dressing, if original position must be passed through on the surface of a wound by applying
Plastic method can not save use, and due to plastic excessive velocities, NaOH solution is difficult to be uniformly mixed, and causes pH value that can not control
System.Therefore it is contemplated that being dropped evenly on gauze after chitosan solution is mixed in proportion with metal ion solution, by cold
The chitosan-based gauze dressing of load multi-metal ion is made in jelly method, which may be directly applied to the surface of a wound, or first be made negative
The gauze dressing for carrying substance metal ion, by stacking layer by layer, control metal ion successively discharges, thus wound healing not
Same time plays the role of corresponding.
Summary of the invention
In view of the above problems, it is an object of the invention to provide a kind of chitosan-based gauze dressing of loaded metal ion, the yarn
The metal ion of cloth dressing load can be added as needed one or more, may be directly applied to the surface of a wound, can also pass through layer stackup
It is applied after putting.The concentration of metal ions of load can be adjusted by changing the metal ion solution concentration prepared.
The present invention provides a kind of chitosan gauze dressing for loading multi-metal ion, and the metal ion is selected from Ag+、Ca2 +、Zn2+、Cu2+、Fe3+、Mg2+One or more of, it can be selected and be arranged in pairs or groups as needed, it is found that it passes through freezing
It can plastic.All include a variety of chitosan-metal ions in gauze dressing obtained in this embodiment, can be applied in the surface of a wound,
Facilitate wound repair.
Preferably, the metal ion includes Ag in the specific embodiment of the present invention+、Ca2+、Zn2+、Cu2 +、Fe3+、Mg2+It is two or more, antibiotic property is substantially better than simple chitosan gauze dressing；
In the present invention, other are preferably carried out in mode, and the present invention provides a kind of chitosan of multilayer loaded metal ion
The metal ion of base gauze dressing, the chitosan-based gauze dressing of every layer of loaded metal ion can be selected from Ag+、Ca2+、Zn2+、Cu2 +、Fe3+、Mg2+One or more of Deng；The gauze dressing is also directly applicable to the surface of a wound, or by stacking load layer by layer not
With the gauze dressing of metal ion, this method controls gauze dressing and discharges corresponding metal ion in the different phase of wound healing
Carry out wound healing.
Preferably, providing a kind of chitosan of three layers of loaded metal ion in a specific embodiment of the invention
Base gauze dressing, loaded Ag+And Ca2+It is one layer, loads Zn2+It is one layer, loaded Cu2+It is one layer, is needed according to different by three layers
Gauze dressing stacks layer by layer, it is made to play respective action in wound repair.
Preferably, above-mentioned three kinds of gauze dressings are folded in the following order in another embodiment of the present invention
It puts: the chitosan-based gauze dressing loaded Ag of first layer loaded metal ion+And Ca2+, it is used for antibacterial and hemostasis；Second layer load
The chitosan-based gauze dressing of metal ion loads Zn2+, immune for stimulating；The chitosan-based yarn of third layer loaded metal ion
Cloth dressing loaded Cu2+, for promoting angiogenesis etc.；Gauze dressing is controlled by this method to release in the different phase of wound healing
It puts corresponding metal ion and carrys out wound healing.
The gauze dressing of load multi-metal ion provided by the invention, ingredient includes the chitosan of loaded metal ion
Gel and water, it is bonded with coordinate bond between chitosan and metal ion；The chitosan gel rubber general structure of loaded metal ion are as follows:
Mn+It in this invention include: Ag for metal ion+, Ca2+, Cu2+, Zn2+, Mg2+, Fe3+Deng；It is as described above negative
Carry the chitosan-based gauze dressing of multi-metal ion, the Viscosity of Chitosan 100-200mpa.s, deacetylation >=95%；It should
Dressing may be directly applied to the surface of a wound, or the gauze dressing of load substance metal ion is first made, and by stacking layer by layer, control metal
Ion successively discharges, thus wound healing different times play the role of it is corresponding.
Another object of the present invention is to provide a kind of preparation method of the chitosan gauze dressing of loaded metal ion, be somebody's turn to do
Method includes the following steps:
It is 0.1~1% chitosan-acetic acid solution solution that step 1), which prepares mass fraction,；
Step 2) prepares at least one set of metal ion solution, and the metal ion in every group of metal ion solution is selected from Ag+, Ca2 +, Cu2+, Zn2+, Mg2+, Fe3+One or more of Deng；
The chitosan solution that step 3) obtains at least one set metal ion solution obtained in step 2) and step 1) is mixed
It closes, chitosan-metal ion solution is made；After solution obtained in step 3) is dispersed on gauze by step 4), at it
Upper covering fixing piece；
The sample that step 5) obtains step 4) be placed under freezing environment freeze after, on gauze uniform fold carried metal from
The hydrogel of son.
In certain preferred embodiments of the present invention, the present invention provides the system of the chitosan gauze dressing of loaded metal ion
Preparation Method, the freezing environment in the step 5) are lower than -20 DEG C；
In certain preferred embodiments of the present invention, the freezing environment in step 5) is -20 DEG C optional, and -80 DEG C, liquid nitrogen；
Preferably -80 DEG C；It is freezed it is furthermore preferred that can be placed in Different hypothermia Gradient, such as: it is respectively put in 4 DEG C, -20 DEG C, -80 DEG C
8h is set, finally saves and places in -80 DEG C.
The present inventor has found that should prepare mass fraction is that 0.1~1% chitosan-acetic acid solution is molten after study
Liquid；Acetate concentration is 0.05~0.5%), if acetate concentration is lower than 0.05%, the solubility of chitosan is greatly reduced, thus
Last plastic state is influenced, if acetate concentration is higher than 0.5%, solution PH is too low, is unfavorable for the environment of wound repair.At this
In invention certain preferred embodiments, the solution of the chitosan is that concentration is 0.5%, and is contained in the chitosan solution
0.1% acetic acid；
In the still other preferred embodiment of the present invention, the loading concentrations of the metal ion are 0.0025~0.01mol/
L；Such as when containing multi-metal solion, the loading concentrations of each metal ion are 0.0025mol/L or 0.005mol/
L, when containing a metal ion species, the loading concentrations of metal ion are 0.01mol/L, introduction according to the present invention, load from
The concentration of son can do a variety of replacements.
The present invention it is some be preferably carried out in mode, in the step 2): prepare one group of metal ion solution, the gold
The metal ion belonged in solion is selected from Ag+, Ca2+, Cu2+, Zn2+, Mg2+, Fe3+One or more of Deng；
In other preferred embodiments of the invention, in the step 2), configuration multiple groups metal ion solution, every group
Metal ion in metal ion solution is selected from Ag+, Ca2+, Cu2+, Zn2+, Mg2+, Fe3+One or more of Deng, then will
It is mixed to get the chitosan-acetic acid solution containing multi-metal ion with chitosan solution；In a specific embodiment party of the invention
In formula, compound concentration is the Ag of 0.1mol/L+, Ca2+, Zn2+, Cu2+Solution；Take the above-mentioned each solion and 900ul of 25ul
Chitosan solution mixes, and chitosan-metal ion solution containing multi-metal ion is made, at this time the load of each metal ion
Concentration is 0.0025mol/L；It is mixed after preparing with chitosan solution, finally obtains the chitosan of load multi-metal ion
Gauze dressing；
In the still other more preferably embodiment of the present invention, in the step 2): preparing multiple groups metal ion solution, often
Metal ion in group metal ion solution is selected from Ag+, Ca2+, Cu2+, Zn2+One or more of；By every group of metal ion
Solution and chitosan-acetic acid solution carry out being mixed to get multiple groups metal ion-chitosan mixed solution, pass through step 3)-step 5)
The chitosan gauze dressing of multiple groups loaded metal ion is obtained, is stacked layer by layer after multiple groups gauze dressing is stacked or cut layer by layer
Obtain the chitosan gauze dressing of multilayer loaded metal ion；In a specific embodiment of the invention, in the step 2),
Three groups of metal ion solutions are configured, the metal ion in first group of metal ion solion is Ag+And Ca2+；Second group of metal
The metal ion of solion is Zn2+；The metal ion of third group metal ion solution is Cu2+；Every group of metal ion solution warp
Cross step 3)~5) obtain the gauze dressing of three groups of loaded metal ions, first group of gauze dressing loaded metal ion Ag+And Ca2 +；Second group of gauze dressing loaded metal ion Zn2+；Third group gauze dressing loaded metal ion Cu2+；Layer by layer by three layers of gauze
It stacks, specifically: first group of gauze dressing is placed on first layer for antibacterial and hemostasis；Second group of gauze dressing is placed on second
Layer is immune for stimulating；Third group gauze dressing is placed on third layer for promoting angiogenesis etc.；Yarn is controlled by this method
Cloth dressing discharges corresponding metal ion in the different phase of wound healing and carrys out wound healing；Specifically at one of the invention
In embodiment, above-mentioned Ag+With Ca2+Loading concentrations be 0.005mol/L, Zn2+Loading concentrations be 0.01mol/L；Cu2+It is negative
Load concentration is 0.01mol/L.
In some specifically embodiments of the present invention, the step 4) and specific embodiment 5) are as follows: in glass slide
The polyfluortetraethylene plate of one piece of intermediate hollow out is placed above, hollow part places the gauze cut in advance, by preparation in step 3)
Good solution drops evenly on gauze；Step 5) covers one piece of glass slide above the gauze that step 4) obtains, and uses clip
The device is fixed, respectively places 8h in 4 DEG C, -20 DEG C, -80 DEG C；Device is taken out, at room temperature device for opening after rewarming, it can
See on gauze the uniform fold hydrogel of loaded metal ion.
The preparation method of the gauze dressing of loaded metal ion provided by the invention, it is creative by chitosan solution and metal
Solion drops evenly on gauze after mixing in proportion, and the chitosan-based of load multi-metal ion is made by freezing
Gauze dressing, freezing reinforces metal ion and the coordinate bond of chitosan acts on, and uniform hydrogel is formed on gauze；It solves
It (is difficult to mix, hydrogel poor mechanical property, applied to gel in-situ must be passed through on the surface of a wound using the defect of NaOH solution plastic
The problems such as), gauze dressing preparation method provided by the invention without adjust PH can uniform plastic, can be reserved for place；The present invention
Easy to operate, material is easy to get, and utilization rate is high, by changing the i.e. adjustable load capacity of metal ion solution concentration；The present invention is made
Hydrogel be covered in uniformly and firmly gauze, therefore use can be saved at low temperature.
And the dressing that this method obtains may be directly applied to the surface of a wound, or first be made load substance metal ion gauze apply
Material, by stacking layer by layer, control metal ion successively discharges, thus wound healing different times play the role of it is corresponding.
Detailed description of the invention
Fig. 1 is the process for the gauze dressing that freezing provided by the invention prepares loaded metal ion.
Fig. 2 is the schematic diagram that the gauze dressing that the prioritization scheme in the present invention stacks layer by layer is applied to the surface of a wound.
Fig. 3 is using the wounds in mice size statistical chart after different gauze dressings；Basis is giving the -2nd of infection respectively
It, surface of a wound at the 0th, 7,10,14,17 day of administration carries out counting statistics.
Fig. 4 is the agar coated plate figure of the 2nd, 4,7 day mouse infection wound fluid upon administration.
Specific embodiment
Below by each example embodiment is described, the present invention is more specifically illustrated.Pass through the operating procedure to the invention
Be described, reflect this law it is easy, practical, application is strong the advantages that.
Embodiment 1
This example, which provides, a kind of prepares loaded Ag+And Ca2+Chitosan-based gauze dressing method, include the following steps:
Step 1) weighs 0.25g Chitosan powder and is dissolved in the acetum of 50ml 0.1%, and compound concentration is 0.5%
Chitosan solution is placed in ultrasonic machine ultrasound 6h；
Step 2) compound concentration is the AgNO of 0.1mol/L3, CaSO4Solution is kept in dark place；
Step 3) takes 50ulAg+And 50ulCa2+Solution is uniformly mixed with 900ul chitosan solution, is made containing Ag+With
Ca2+Chitosan-metal ion solution, i.e. Ag+With Ca2+Loading concentrations be 0.005mol/L；
Glass slide is placed on by step 4), in the polyfluortetraethylene plate that one piece of intermediate hollow out is placed above, hollow out
The gauze cut in advance is placed at place, and the solution prepared in step 3) is dropped evenly on gauze；One piece is covered above
Glass slide, and fixed the device with clip；
The sample that step 5) obtains step 4) respectively places freezing 8h in 4 DEG C, -20 DEG C, -80 DEG C, finally in -80 DEG C
It saves and places；Device is taken out, at room temperature device for opening after rewarming, it can be seen that uniform fold loaded metal ion on gauze
Hydrogel.
Embodiment 2
This example provides a kind of prepare and loads Zn2+Chitosan-based gauze dressing method, specifically
Step 1) weighs 0.25g Chitosan powder and is dissolved in 0.1% acetum, and the shell that compound concentration is 0.5% is poly-
Sugar juice is placed in ultrasonic machine ultrasound 6h；
Step 2) compound concentration is the Zn (NO of 0.1mol/L3)2Solution is kept in dark place；
Step 3) takes 100ul Zn2+Solution is uniformly mixed with 900ul chitosan solution, is made containing Zn2+Chitosan-
Metal ion solution, at this time Zn2+Loading concentrations be 0.01mol/L；
Glass slide is placed on by step 4), in the polyfluortetraethylene plate that one piece of intermediate hollow out is placed above, hollow out
The gauze cut in advance is placed at place, and the solution prepared in step 3) is dropped evenly on gauze；One piece is covered above
Glass slide, and fixed the device with clip；
Freezing 8h is respectively placed in 4 DEG C of sample, -20 DEG C, -80 DEG C that step 5) obtains step 4), is finally protected in -80 DEG C
Storage is set；Device is taken out, at room temperature device for opening after rewarming, it can be seen that uniform fold loaded metal ion on gauze
Hydrogel.
Embodiment 3
This example, which provides, a kind of prepares loaded Cu2+Chitosan-based gauze dressing,
Step 1) weighs 0.25g Chitosan powder and is dissolved in 0.1% acetum, and the shell that compound concentration is 0.5% is poly-
Sugar juice is placed in ultrasonic machine ultrasound 6h；
Step 2) compound concentration is the CuSO of 0.1mol/L4Solution is kept in dark place；
Step 3) takes 100ul Cu2+Solution is uniformly mixed with 900ul chitosan solution, is made containing Cu2+Chitosan-
Metal ion solution, at this time Cu2+Loading concentrations be 0.01mol/L；
Glass slide is placed on by step 4), in the polyfluortetraethylene plate that one piece of intermediate hollow out is placed above, hollow out
The gauze cut in advance is placed at place, and the solution prepared in step 3) is dropped evenly on gauze；
One piece of glass slide is covered above, and is fixed the device with clip；
Freezing 8h is respectively placed in 4 DEG C of sample, -20 DEG C, -80 DEG C that step 5) obtains step 4), is finally protected in -80 DEG C
Storage is set；Device is taken out, at room temperature device for opening after rewarming, it can be seen that uniform fold loaded metal ion on gauze
Hydrogel.
Embodiment 4
Embodiment 1, embodiment 2 and embodiment 3 are obtained into three kinds of gauze dressings and are cut into 0.8cm x 0.8cm size
Square dressing, by loaded Ag+And Ca2+Gauze be placed on first layer, load Zn2+Gauze be placed on the second layer, loaded Cu2 +Gauze be placed on third layer, form the gauze dressing for discharging different metal ions layer by layer.
Embodiment 5
This example provides a kind of prepare while loaded Ag+, Ca2+, Zn2+, Cu2+Chitosan-based gauze dressing.
Step 1) weighs 0.25g Chitosan powder and is dissolved in 0.1% acetum, and the shell that compound concentration is 0.5% is poly-
Sugar juice is placed in ultrasonic machine ultrasound 6h；
Step 2) compound concentration is the Ag of 0.1mol/L+, Ca2+, Zn2+, Cu2+Solution is kept in dark place；
Step 3) take 25ul above-mentioned each solion and 900ul chitosan solution mix, be made containing multi-metal from
Chitosan-metal ion solution of son, the loading concentrations of each metal ion are 0.0025mol/L at this time；
Glass slide is placed on by step 4), in the polyfluortetraethylene plate that one piece of intermediate hollow out is placed above, hollow out
The gauze cut in advance is placed at place, and the solution prepared in step 3) is dropped evenly on gauze；One piece is covered above
Glass slide, and fixed the device with clip；
Freezing 8h is respectively placed in 4 DEG C of sample, -20 DEG C, -80 DEG C that step 5) obtains step 4), is finally protected in -80 DEG C
Storage is set；Device is taken out, at room temperature device for opening after rewarming, it can be seen that uniform fold loaded metal ion on gauze
Hydrogel.
Gauze dressing in embodiment 5 is cut into the square dressing of 0.8cm x 0.8cm size, which can release simultaneously
Put multi-metal ion.
Embodiment 6
This example provides a kind of prepare and loads Fe3+Chitosan-based gauze dressing method, specifically
Step 1) weighs 0.25g Chitosan powder and is dissolved in 0.1% acetum, and the shell that compound concentration is 0.5% is poly-
Sugar juice is placed in ultrasonic machine ultrasound 6h；
Step 2) compound concentration is the FeCl of 0.1mol/L3Solution is kept in dark place；
Step 3) takes 100ul Fe3+Solution is uniformly mixed with 900ul chitosan solution, is made containing Fe3+Chitosan-
Metal ion solution, at this time Fe3+Loading concentrations be 0.01mol/L；
Glass slide is placed on by step 4), in the polyfluortetraethylene plate that one piece of intermediate hollow out is placed above, hollow out
The gauze cut in advance is placed at place, and the solution prepared in step 3) is dropped evenly on gauze；One piece is covered above
Glass slide, and fixed the device with clip；
Freezing 8h is respectively placed in 4 DEG C of sample, -20 DEG C, -80 DEG C that step 5) obtains step 4), is finally protected in -80 DEG C
Storage is set；Device is taken out, at room temperature device for opening after rewarming, it can be seen that uniform fold loaded metal ion on gauze
Hydrogel.
Embodiment 7
This example provides a kind of prepare and loads Mg2+Chitosan-based gauze dressing method, specifically
Step 1) weighs 0.25g Chitosan powder and is dissolved in 0.1% acetum, and the shell that compound concentration is 0.5% is poly-
Sugar juice is placed in ultrasonic machine ultrasound 6h；
Step 2) compound concentration is the MgCl of 0.1mol/L2Solution is kept in dark place；
Step 3) takes 100ul Mg2+Solution is uniformly mixed with 900ul chitosan solution, is made containing Mg2+Chitosan-
Metal ion solution, at this time Mg2+Loading concentrations be 0.01mol/L；
Glass slide is placed on by step 4), in the polyfluortetraethylene plate that one piece of intermediate hollow out is placed above, hollow out
The gauze cut in advance is placed at place, and the solution prepared in step 3) is dropped evenly on gauze；One piece is covered above
Glass slide, and fixed the device with clip；
Freezing 8h is respectively placed in 4 DEG C of sample, -20 DEG C, -80 DEG C that step 5) obtains step 4), is finally protected in -80 DEG C
Storage is set；Device is taken out, at room temperature device for opening after rewarming, it can be seen that uniform fold loaded metal ion on gauze
Hydrogel.
Test example 1
The gauze of chitosan alone, the manufactured gauze dressing placed layer by layer and reality in above-described embodiment 4 will be added dropwise
The gauze dressing for applying the load multiple ion in example 5 is respectively applied on the wounds in mice of S. aureus L-forms infection.Using S. aureus L-forms
The surface of a wound of each group mouse was assessed in the 7th, 10,14,17 day after infection and after surface of a wound administration, statistics is as shown in Fig. 2, use
What embodiment 4 obtained is discharged the gauze dressing of different metal ions layer by layer and discharges multiple gold while being obtained using embodiment 5
The healing speed for belonging to the gauze dressing of ion is very fast, and the healing speed of chitosan alone is used to be far below preceding two
Group.
Test example 2
The 17th day after surface of a wound administration wound healing situation is described, when mouse is administered the 2nd, 4,7 day, is used respectively
Aseptic cotton carrier, which is wiped, takes juice on every group of wounds in mice, with after normal saline dilution by it in being coated on agar plate, as a result
As shown in figure 3, shown in reference table 1, it is known that the 2.3rd group of the surface of a wound was significantly reduced in second day bacterium amount, and in the 1st group of the surface of a wound
Bacterium amount be always maintained at highest,
Effect of each gauze dressing of table 1 to the surface of a wound
It is tested by the above wound healing it is found that the gauze dressing that embodiment 4 and embodiment 5 obtain, antibiotic property are obviously big
In existing chitosan alone gauze dressing, and as Fig. 3 can be seen that the yarn for the release metal ions layer by layer that embodiment 4 obtains
Cloth dressing antibiotic property is substantially better than embodiment 5 and obtains gauze dressing.
Test example 3
The gauze of chitosan alone will be added dropwise, the gauze dressing in above-described embodiment 6 and embodiment 7 is respectively applied to small
On the mouse surface of a wound.The surface of a wound of each group mouse was assessed in the 17th day after surface of a wound administration.
Effect of each gauze dressing of table 2 to the surface of a wound
It is tested by the above wound healing it is found that the gauze dressing that embodiment 6 and embodiment 7 obtain, wound healing
Effect be significantly greater than existing chitosan alone gauze dressing.
Claims (10)
1. a kind of chitosan-based gauze dressing of loaded metal ion, it is characterised in that: gauze dressing load has metal ion,
The metal ion is selected from Ag+、Ca2+、Zn2+、Cu2+、Fe3+、Mg2+One or more of.
2. the chitosan-based gauze dressing of loaded metal ion according to claim 1, it is characterised in that: the metal from
Attached bag contains Ag+、Ca2+、Zn2+、Cu2+、Fe3+、Mg2+It is two or more.
3. a kind of chitosan-based gauze dressing of multilayer loaded metal ion, wherein the shell of the multilayer loaded metal ion is poly-
Glycosyl gauze dressing is made of the chitosan-based gauze dressing of multiple groups loaded metal ion as described in claim 1, wherein institute
The chitosan-based gauze dressing for stating multiple groups loaded metal ion stacks layer by layer.
4. the chitosan gauze dressing of multilayer loaded metal ion according to claim 4, it is characterised in that: the multilayer
The chitosan-based gauze dressing of loaded metal ion is the chitosan-based gauze dressing of three layers of loaded metal ion, first layer is negative
The chitosan-based gauze dressing loaded Ag of carrying metal ion+And Ca2+；The chitosan-based gauze dressing of second layer loaded metal ion
Load Zn2+；The chitosan-based gauze dressing loaded Cu of third layer loaded metal ion2+。
5. the preparation method of the chitosan-based gauze dressing of loaded metal ion according to claim 1, it is characterised in that:
It is 0.1~1% chitosan solution that step 1), which prepares mass fraction,；Step 2) prepares at least one set of metal ion solution, every group of gold
The metal ion belonged in solion is selected from Ag+、Ca2+、Zn2+、Cu2+、Fe3+、Mg2+One or more of；Step 3) will walk
It is rapid 2) obtained at least one set metal ion solution mixed with the chitosan solution that step 1) obtains, chitosan-metal is made
Solion；After solution obtained in step 3) is dispersed on gauze by step 4), fixing piece is covered on it；Step 5)
The sample that step 4) is obtained is placed under freezing environment after freezing, the hydrogel of uniform fold loaded metal ion on gauze.
6. the preparation method of the chitosan-based gauze dressing of loaded metal ion according to claim 5, it is characterised in that:
Freezing environment in the step 5) is lower than -20 DEG C.
7. the chitosan-based gauze dressing preparation method of loaded metal ion according to claim 5, it is characterised in that: institute
It is 0.5% that the solution of chitosan, which is concentration, in the step 1), and in the chitosan solution containing 0.1% acetic acid.
8. the preparation method of the chitosan-based gauze dressing of loaded metal ion according to claim 5, it is characterised in that:
Prepare Ag+, Ca2+, Zn2+, Cu2+Solution；Take above-mentioned each solion and chitosan solution to mix, be made containing multi-metal from
Chitosan-metal ion solution of son finally obtains the chitosan gauze dressing of load multi-metal ion.
9. the preparation method of the chitosan-based gauze dressing of loaded metal ion according to claim 5, it is characterised in that:
Three groups of metal ion solutions are configured, the metal ion in first group of metal ion solion is Ag+And Ca2+；Second group of metal
The metal ion of solion is Zn2+；The metal ion of third group metal ion solution is Cu2+；Every group of metal ion solution warp
Cross step 3)~5) obtain the gauze dressing of three groups of loaded metal ions, first group of gauze dressing loaded metal ion Ag+And Ca2 +；Second group of gauze dressing loaded metal ion Zn2+；Third group gauze dressing loaded metal ion Cu2+；Then by three groups of gauzes
Dressing layer stackup is put.
10. the preparation method of the chitosan-based gauze dressing of loaded metal ion according to claim 5, feature exist
In: the loading concentrations of the metal ion are 0.0025~0.01mol/L.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811115484.9A CN109010898B (en) | 2018-09-25 | 2018-09-25 | Metal ion loaded chitosan-based gauze dressing and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811115484.9A CN109010898B (en) | 2018-09-25 | 2018-09-25 | Metal ion loaded chitosan-based gauze dressing and preparation method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN109010898A true CN109010898A (en) | 2018-12-18 |
| CN109010898B CN109010898B (en) | 2021-07-20 |
Family
ID=64617839
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811115484.9A Active CN109010898B (en) | 2018-09-25 | 2018-09-25 | Metal ion loaded chitosan-based gauze dressing and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109010898B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116355258A (en) * | 2021-12-20 | 2023-06-30 | 北京健康广济生物技术有限公司 | Preparation method of chitosan film and sodium carboxymethyl cellulose hydrogel burn dressing based on double-layer structure |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102120043A (en) * | 2011-02-23 | 2011-07-13 | 中国人民解放军军事医学科学院基础医学研究所 | Absorbent cotton gauze containing nanometer silver and chitosan, preparation device as well as method thereof |
| CN102847193A (en) * | 2012-07-04 | 2013-01-02 | 杭州科洛生物技术有限公司 | Chitosan fluid dressing film |
| CN104080487A (en) * | 2012-01-19 | 2014-10-01 | Cg生物技术有限公司 | Antimicrobial wound-covering material and method for manufacturing same |
| CN105641733A (en) * | 2015-12-29 | 2016-06-08 | 山东康力医疗器械科技有限公司 | Preparation method for compound antibacterial haemostatic wound dressing |
| BR102015029357A2 (en) * | 2015-11-24 | 2017-12-05 | Polymar Indústria E Comércio Importação E Exportação Ltda | THE USE OF BANDAGES AND MEMBRANES PRODUCED WITH QUITOSAN AND COMBINED DERIVATIVES WITH VARIOUS ADDITIVES IN THE REGENERATION OF INJURED FABRICS AND HEALING |
| CN108434507A (en) * | 2018-04-25 | 2018-08-24 | 南开大学 | Ion mediation dressing and its preparation method and application |
-
2018
- 2018-09-25 CN CN201811115484.9A patent/CN109010898B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102120043A (en) * | 2011-02-23 | 2011-07-13 | 中国人民解放军军事医学科学院基础医学研究所 | Absorbent cotton gauze containing nanometer silver and chitosan, preparation device as well as method thereof |
| CN104080487A (en) * | 2012-01-19 | 2014-10-01 | Cg生物技术有限公司 | Antimicrobial wound-covering material and method for manufacturing same |
| CN102847193A (en) * | 2012-07-04 | 2013-01-02 | 杭州科洛生物技术有限公司 | Chitosan fluid dressing film |
| BR102015029357A2 (en) * | 2015-11-24 | 2017-12-05 | Polymar Indústria E Comércio Importação E Exportação Ltda | THE USE OF BANDAGES AND MEMBRANES PRODUCED WITH QUITOSAN AND COMBINED DERIVATIVES WITH VARIOUS ADDITIVES IN THE REGENERATION OF INJURED FABRICS AND HEALING |
| CN105641733A (en) * | 2015-12-29 | 2016-06-08 | 山东康力医疗器械科技有限公司 | Preparation method for compound antibacterial haemostatic wound dressing |
| CN108434507A (en) * | 2018-04-25 | 2018-08-24 | 南开大学 | Ion mediation dressing and its preparation method and application |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116355258A (en) * | 2021-12-20 | 2023-06-30 | 北京健康广济生物技术有限公司 | Preparation method of chitosan film and sodium carboxymethyl cellulose hydrogel burn dressing based on double-layer structure |
Also Published As
| Publication number | Publication date |
|---|---|
| CN109010898B (en) | 2021-07-20 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105683426B (en) | The method for preparing polymers capable of swelling fiber | |
| US4959341A (en) | Biodegradable superabsorbing sponge | |
| US10624982B2 (en) | Wound care compositions | |
| CA2960039C (en) | Medical dressing | |
| US5073202A (en) | Method of using a biodegradable superabsorbing sponge | |
| CN103961738B (en) | A kind of chitosan-nanometer silver wound dressing and preparation method thereof | |
| CN105268015A (en) | Antibacterial hydrogel composite material and preparation method thereof | |
| DE10330971B4 (en) | Process for the production of skin or wound dressings with encapsulated, wound-healing and / or skin-care substances | |
| CN100427149C (en) | Nano silver bionic dressing and its preparing method | |
| EP3258974A1 (en) | Hemostatic composition and hemostatic device (variants) | |
| CN105709262A (en) | Silver-loaded antibacterial dressing and preparation method thereof | |
| EP2185206A2 (en) | Haemostatic wound care article | |
| JP2008503286A (en) | Wound dressing | |
| Li et al. | Fabrication of Ag–ZnO@ carboxymethyl cellulose/K-carrageenan/graphene oxide/konjac glucomannan hydrogel for effective wound dressing in nursing care for diabetic foot ulcers | |
| DE10330960B4 (en) | Active substance-doped absorbent polymer particles, composition comprising polycondensate matrix and absorbent polymer for release of a wound healing substance | |
| CN109010898A (en) | A kind of chitosan-based gauze dressing of loaded metal ion and preparation method thereof | |
| CN106474526B (en) | A kind of amorphous bearing hydrocolloid dressing and preparation method thereof containing liposome | |
| Nagrath et al. | Porcine liver injury model to assess tantalum-containing bioactive glass powders for hemostasis | |
| WO2021027219A1 (en) | Absorbable bone wax and preparation method therefor | |
| CN105363067B (en) | A kind of hemostasis sterilization wound treatment agent and preparation method thereof | |
| GB2609541A (en) | Antimicrobial wound dressing | |
| CN108124904A (en) | A kind of histone-load silver complex antimicrobials and preparation method | |
| DE102011013616A1 (en) | Wound dressing used in vacuum treatment, comprises wound contact surface with hydrophilic layer formed on support material, which is smooth and mechanically stable, where water film is attached on wound contact surface with high stability |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |