CN109010399A - A kind of preparation method of plant cell wall breaking powder - Google Patents
A kind of preparation method of plant cell wall breaking powder Download PDFInfo
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- CN109010399A CN109010399A CN201810788805.5A CN201810788805A CN109010399A CN 109010399 A CN109010399 A CN 109010399A CN 201810788805 A CN201810788805 A CN 201810788805A CN 109010399 A CN109010399 A CN 109010399A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/25—Araliaceae (Ginseng family), e.g. ivy, aralia, schefflera or tetrapanax
- A61K36/258—Panax (ginseng)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/10—Preparation or pretreatment of starting material
- A61K2236/15—Preparation or pretreatment of starting material involving mechanical treatment, e.g. chopping up, cutting or grinding
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/10—Preparation or pretreatment of starting material
- A61K2236/19—Preparation or pretreatment of starting material involving fermentation using yeast, bacteria or both; enzymatic treatment
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- General Health & Medical Sciences (AREA)
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- Coloring Foods And Improving Nutritive Qualities (AREA)
- Preparation Of Fruits And Vegetables (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention discloses a kind of preparation methods of plant cell wall breaking powder, comprising the following steps: (1) takes plant material, clean, clean；(2) crushing material for obtaining step (1) is not more than the particulate matter of 5mm at material specification, and dry；(3) step (2) acquisition particulate matter is pulverized, the ultra tiny powder that particle diameter distribution D90 is 10~20um is made；(4) citric acid solution and Broken kernel enzyme is added in the ultra tiny powder obtained to step (3), stirs evenly, 10~30min is digested at 45~55 DEG C；(5) step (4) are obtained into enzymolysis liquid centrifugation, takes supernatant, be freeze-dried to get the breaking-wall cell powder.The application has found, is suitably digested after ultramicro grinding to ultra tiny powder, can effectively improve sporoderm-broken rate, has low cost, high sporoderm-broken rate and simple operation and other advantages.
Description
Technical field
The invention belongs to plant processing technique fields, and in particular to a kind of preparation method of plant cell wall breaking powder.
Background technique
Plant cell wall is due to being constituted it, it is extremely difficult to be digested by human body gastric acid, so the effective component of medicinal material is thin
After cell wall package be difficult effectively to excite, and after cell wall broken wall, can will make to be wrapped tightly by outer wall in small molecular state effectively at
Divide the utilization that can farthest be absorbed by the body, the excitation drug effect of help improves human body to medicinal material absorptivity.
After broken wall, the effective component in cell wall sufficiently exposes plant medicinal material, and the effective component of plant is released
Putting speed and burst size can increase substantially, so that the infiltration rate of human body returns obvious quickening, uptake can also be obviously increased.Mesh
Before there are various plants cell wall breaking technologies can be divided into Mechanical Method and on-mechanical method according to whether there are applied external forces.According to
Physics and non-physical method can be divided into physical method and chemical method.Physical method mainly has: can be divided into low temperature object according to broken wall temperature
Manage broken wall, ultralow temperature physical wall breaking；High-speed impact broken wall, shearing force broken wall etc., non-physical method can be divided into according to firmly situation
Mainly there are chemical-agent technique and biologic enzymolysis method.But there are still some defects for existing wall breaking technology, and sporoderm-broken rate is not high,
So that drug drug effect excitation or it is very limited.
Summary of the invention
Based on this, it is broken that a kind of plant cell is provided it is an object of the invention to overcome in place of above-mentioned the deficiencies in the prior art
The preparation method of wall powder can effectively improve sporoderm-broken rate, have low cost, high sporoderm-broken rate and simple operation and other advantages.
To achieve the above object, the technical solution adopted by the present invention are as follows: a kind of preparation method of plant cell wall breaking powder,
It is characterized in that, comprising the following steps:
(1) plant material is taken, cleans, clean；
(2) crushing material for obtaining step (1) is not more than the particulate matter of 5mm at material specification, and dry；
(3) particulate matter that step (2) obtain is pulverized, the ultra micro that particle diameter distribution D90 is 10~20um is made
Fine silt；
(4) citric acid solution and Broken kernel enzyme is added in the ultra tiny powder obtained to step (3), stirs evenly, in
10~30min is digested at 45~55 DEG C；
(5) step (4) are obtained into enzymolysis liquid centrifugation, takes supernatant, be freeze-dried to get the breaking-wall cell powder.
Preferably, plant is integration of drinking and medicinal herbs class plant in the step (1).
It preferably, is 10% or less by particulate matter drying to moisture content in the step (2).
It is highly preferred that drying particulate matter to moisture content in the step (2) is 7% or less.
Preferably, it is 0~8 DEG C that temperature is pulverized in the step (3), and the time is 0.5~1h.
It is highly preferred that it is 5 DEG C that temperature is pulverized in the step (3), time 0.8h.
Preferably, the w/v of ultra tiny powder and citric acid solution is 1g:(1~3 in the step (4)) mL；
The pH value of the citric acid solution is 5~6.
It is highly preferred that the w/v of ultra tiny powder and citric acid solution is 1g:2mL in the step (4).
Preferably, the usage amount of Broken kernel enzyme is 1200~1800U/g in the step (4).
Preferably, the usage amount of Broken kernel enzyme is 1500U/g in the step (4).
Preferably, the Broken kernel enzyme includes cellulase and hemicellulase, and the cellulase and hemicellulose
The enzyme activity ratio of plain enzyme is (2~5): 1.
Preferably, the enzyme activity of the cellulase and hemicellulase ratio is 3:1.
Preferably, 20min is digested at 50 DEG C in the step (5).
Compared with the existing technology, the invention has the benefit that the application has found, after ultramicro grinding to ultra tiny powder into
The appropriate enzymatic hydrolysis of row, can effectively improve sporoderm-broken rate, have low cost, high sporoderm-broken rate and simple operation and other advantages.
Specific embodiment
To better illustrate the object, technical solutions and advantages of the present invention, below in conjunction with specific embodiment to the present invention
It is described further.
Embodiment 1
A kind of embodiment of the preparation method of plant cell wall breaking powder of the present invention, comprising the following steps:
(1) ginseng, removal impurity and rotten person are taken；
(2) crushing material for obtaining step (1) is not more than the particulate matter of 5mm at material specification, and is put into heat pump drying
Dehumidifier drying to moisture content is 10% or less；
(3) particulate matter that step (2) obtain is pulverized with atomizer, pulverizing temperature is 0 DEG C, the time
For 1h, the ultra tiny powder that particle diameter distribution D90 is 10~20um is made；
(4) it is 5 citric acid solutions and Broken kernel enzyme, stirring that pH value is added in the ultra tiny powder obtained to step (3)
Uniformly, 30min is digested at 45 DEG C；The w/v of the coarse powder and citric acid solution is 1g:1mL；The breaking-wall cell
The usage amount of enzyme is 1300U/g, and the Broken kernel enzyme includes cellulase and hemicellulase, and the cellulase and half
The enzyme activity ratio of cellulase is 4:1, i.e., is separately added into 1040U cellulase and 260U hemicellulase in every g coarse powder；
(5) step (4) are obtained into enzymolysis liquid centrifugation, takes supernatant, be freeze-dried to get the breaking-wall cell powder.
Embodiment 2
A kind of embodiment of the preparation method of plant cell wall breaking powder of the present invention, comprising the following steps:
(1) ginseng, removal impurity and rotten person are taken；
(2) crushing material for obtaining step (1) is not more than the particulate matter of 5mm at material specification, and is put into heat pump drying
Dehumidifier drying to moisture content is 7% or less；
(3) particulate matter that step (2) obtain is pulverized with atomizer, pulverizing temperature is 8 DEG C, the time
For 0.6h, the ultra tiny powder that particle diameter distribution D90 is 10~20um is made；
(4) pH value is added in the ultra tiny powder obtained to step (3) is 5.6 citric acid solutions and Broken kernel enzyme, is stirred
It mixes uniformly, digests 10min at 55 DEG C；The w/v of the coarse powder and citric acid solution is 1g:2mL；The cell is broken
The usage amount of wall enzyme is 1200U/g, and the Broken kernel enzyme includes cellulase and hemicellulase, and the cellulase and
The enzyme activity ratio of hemicellulase is 2:1, i.e., is separately added into 800U cellulase and 400U hemicellulase in every g coarse powder；
(5) step (4) are obtained into enzymolysis liquid centrifugation, takes supernatant, be freeze-dried to get the breaking-wall cell powder.
Embodiment 3
A kind of embodiment of the preparation method of plant cell wall breaking powder of the present invention, comprising the following steps:
(1) ginseng, removal impurity and rotten person are taken；
(2) crushing material for obtaining step (1) is not more than the particulate matter of 5mm at material specification, and is put into heat pump drying
Dehumidifier drying to moisture content is 7% or less；
(3) particulate matter that step (2) obtain is pulverized with atomizer, pulverizing temperature is 3 DEG C, the time
For 0.5h, the ultra tiny powder that particle diameter distribution D90 is 10~20um is made；
(4) it is 6 citric acid solutions and Broken kernel enzyme, stirring that pH value is added in the ultra tiny powder obtained to step (3)
Uniformly, 15min is digested at 52 DEG C；The w/v of the coarse powder and citric acid solution is 1g:3mL；The breaking-wall cell
The usage amount of enzyme is 1800U/g, and the Broken kernel enzyme includes cellulase and hemicellulase, and the cellulase and half
The enzyme activity ratio of cellulase is 5:1, i.e., is separately added into 1500U cellulase and 300U hemicellulase in every g coarse powder；
(5) step (4) are obtained into enzymolysis liquid centrifugation, takes supernatant, be freeze-dried to get the breaking-wall cell powder.
Embodiment 4
A kind of embodiment of the preparation method of plant cell wall breaking powder of the present invention, comprising the following steps:
(1) ginseng, removal impurity and rotten person are taken；
(2) crushing material for obtaining step (1) is not more than the particulate matter of 5mm at material specification, and is put into heat pump drying
Dehumidifier drying to moisture content is 7% or less；
(3) particulate matter that step (2) obtain is pulverized with atomizer, pulverizing temperature is 5 DEG C, the time
For 0.8h, the ultra tiny powder that particle diameter distribution D90 is 10~20um is made；
(4) pH value is added in the ultra tiny powder obtained to step (3) is 5.5 citric acid solutions and Broken kernel enzyme, is stirred
It mixes uniformly, digests 20min at 50 DEG C；The w/v of the coarse powder and citric acid solution is 1g:2mL；The cell is broken
The usage amount of wall enzyme is 1500U/g, and the Broken kernel enzyme includes cellulase and hemicellulase, and the cellulase and
The enzyme activity ratio of hemicellulase is 3:1, i.e., is separately added into 1125U cellulase and 375U hemicellulase in every g coarse powder；
(5) step (4) are obtained into enzymolysis liquid centrifugation, takes supernatant, be freeze-dried to get the breaking-wall cell powder.
Comparative example 1
Plant cell wall breaking powder is prepared as follows in this comparative example:
(1) ginseng, removal impurity and rotten person are taken；
(2) crushing material for obtaining step (1) is not more than the particulate matter of 5mm at material specification, and is put into heat pump drying
Dehumidifier drying to moisture content is 7% or less；
(3) particulate matter that step (2) obtain is pulverized with atomizer, pulverizing temperature is 5 DEG C, the time
For 0.8h, it is the ultra tiny powder of 10~20um to get the breaking-wall cell powder that particle diameter distribution D90, which is made,
Plant cell wall breaking rate in Examples 1 to 4 and comparative example 1 is detected, the results are shown in Table 1:
1 cell-wall breaking ratio testing result of table
| Group | Sporoderm-broken rate |
| Embodiment 1 | 93.8% |
| Embodiment 2 | 92.5% |
| Embodiment 3 | 95% |
| Embodiment 4 | 98.5% |
| Comparative example 1 | 88.5% |
As shown in Table 1, it can further improve cell to the appropriate enzymatic hydrolysis of ultra tiny powder progress after ultramicro grinding to break
Wall rate, and the preparation method sporoderm-broken rate highest in embodiment 4.
Finally, it should be noted that the above embodiments are merely illustrative of the technical solutions of the present invention rather than protects to the present invention
The limitation of range is protected, although the invention is described in detail with reference to the preferred embodiments, those skilled in the art should
Understand, it can be with modification or equivalent replacement of the technical solution of the present invention are made, without departing from the essence of technical solution of the present invention
And range.
Claims (10)
1. a kind of preparation method of plant cell wall breaking powder, which comprises the following steps:
(1) plant material is taken, cleans, clean；
(2) crushing material for obtaining step (1) is not more than the particulate matter of 5mm at material specification, and dry；
(3) particulate matter that step (2) obtain is pulverized, the micropowders that particle diameter distribution D90 is 10~20um is made
Grain；
(4) citric acid solution and Broken kernel enzyme is added in the ultra tiny powder obtained to step (3), stirs evenly, in 45~
10~30min is digested at 55 DEG C；
(5) step (4) are obtained into enzymolysis liquid centrifugation, takes supernatant, be freeze-dried to get the breaking-wall cell powder.
2. the preparation method of plant cell wall breaking powder according to claim 1, which is characterized in that will in the step (2)
Particulate matter drying to moisture content is 10% or less.
3. the preparation method of plant cell wall breaking powder according to claim 2, which is characterized in that will in the step (2)
Particulate matter drying to moisture content is 7% or less.
4. the preparation method of plant cell wall breaking powder according to claim 1, which is characterized in that surpass in the step (3)
Crushing of Ultrafine temperature is 0~8 DEG C, and the time is 0.5~1h.
5. the preparation method of plant cell wall breaking powder according to claim 1, which is characterized in that surpass in the step (4)
The w/v of fine powder and citric acid solution is 1g:(1~3) mL；The pH value of the citric acid solution is 5~6.
6. the preparation method of plant cell wall breaking powder according to claim 1, which is characterized in that thin in the step (4)
The usage amount of born of the same parents' wall breaking enzyme is 1200~1800U/g.
7. the preparation method of plant cell wall breaking powder according to claim 6, which is characterized in that thin in the step (4)
The usage amount of born of the same parents' wall breaking enzyme is 1500U/g.
8. the preparation method of plant cell wall breaking powder according to claim 6 or 7, which is characterized in that the breaking-wall cell
Enzyme includes cellulase and hemicellulase, and the enzyme activity of the cellulase and hemicellulase ratio is (2~5): 1.
9. the preparation method of plant cell wall breaking powder according to claim 8, which is characterized in that the cellulase and half
The enzyme activity ratio of cellulase is 3:1.
10. the preparation method of plant cell wall breaking powder according to claim 1, which is characterized in that in the step (5) in
20min is digested at 50 DEG C.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| TWI794607B (en) * | 2020-05-22 | 2023-03-01 | 鷗媄吉國際生物科技有限公司 | Microspherulite composition composed of orchid germ extract, and its manufacturing method and use for cell detoxification, energy activation, and aging resistance |
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| CN101884650A (en) * | 2010-07-19 | 2010-11-17 | 江苏丘陵地区镇江农业科学研究所 | Method for producing Chinese herbal medicine powder by using superfine grinding and enzymatic wall-breaking combined technology |
| CN106243106A (en) * | 2016-09-23 | 2016-12-21 | 成都合盛生物技术有限公司 | A kind of method extracting tabersonine from the volt health seeds of trees |
| CN106867657A (en) * | 2017-03-17 | 2017-06-20 | 广州康琪莱生物科技有限公司 | A kind of efficient, multi-stage supercritical CO2The method of Ganoderma Lucidum Spores Oil Extracted |
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2018
- 2018-07-17 CN CN201810788805.5A patent/CN109010399A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101884650A (en) * | 2010-07-19 | 2010-11-17 | 江苏丘陵地区镇江农业科学研究所 | Method for producing Chinese herbal medicine powder by using superfine grinding and enzymatic wall-breaking combined technology |
| CN106243106A (en) * | 2016-09-23 | 2016-12-21 | 成都合盛生物技术有限公司 | A kind of method extracting tabersonine from the volt health seeds of trees |
| CN106867657A (en) * | 2017-03-17 | 2017-06-20 | 广州康琪莱生物科技有限公司 | A kind of efficient, multi-stage supercritical CO2The method of Ganoderma Lucidum Spores Oil Extracted |
Non-Patent Citations (1)
| Title |
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| 梁健钦: "酶法提取联用超微粉碎技术制备桑叶不溶超微颗粒剂及其质量标准研究", 《中药材》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| TWI794607B (en) * | 2020-05-22 | 2023-03-01 | 鷗媄吉國際生物科技有限公司 | Microspherulite composition composed of orchid germ extract, and its manufacturing method and use for cell detoxification, energy activation, and aging resistance |
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